AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0340410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGGGTTCAATATCTTTA+TGG 0.183713 2_4:-61764869 Msa0340410:CDS
TCTCTCGTCGTCACAGCAAA+TGG 0.378944 2_4:-61765687 Msa0340410:CDS
AAGATGTTGATGAAGGTCTA+AGG 0.417135 2_4:-61765789 Msa0340410:CDS
TTCCTTCCTGTTGTCTATTG+TGG 0.433880 2_4:+61765810 None:intergenic
AACAGCGACAAAGCTCTGTT+TGG 0.435533 2_4:-61765745 Msa0340410:CDS
CCACCACCACAATAGACAAC+AGG 0.483309 2_4:-61765816 Msa0340410:CDS
TTGGAGTTGAAAAGGCGATC+TGG 0.488634 2_4:+61765721 None:intergenic
CTCTCGTCGTCACAGCAAAT+GGG 0.488740 2_4:-61765686 Msa0340410:CDS
CAGGAACGAAGATTGCAGAT+TGG 0.496990 2_4:-61764908 Msa0340410:CDS
TGTGGTCGTTGGAGTTGAAA+AGG 0.508406 2_4:+61765713 None:intergenic
CACCGCTGTCTATGCGGCGA+TGG 0.515264 2_4:-61765874 Msa0340410:CDS
CTTCCTGTTGTCTATTGTGG+TGG 0.519411 2_4:+61765813 None:intergenic
CCTGTTGTCTATTGTGGTGG+TGG 0.526419 2_4:+61765816 None:intergenic
TGTCTATGCGGCGATGGTGA+CGG 0.564115 2_4:-61765868 Msa0340410:CDS
GCGATGGTGACGGTGGTGCT+AGG 0.569932 2_4:-61765858 Msa0340410:CDS
TTGTCTATTGTGGTGGTGGT+CGG 0.572841 2_4:+61765820 None:intergenic
ATATTGAACCCTCACTTGTA+CGG 0.574872 2_4:+61764877 None:intergenic
TAAATGCGGAACTAGAAAGA+TGG 0.577341 2_4:+61764830 None:intergenic
AGGAAGGAAGATGTTGATGA+AGG 0.579857 2_4:-61765796 Msa0340410:CDS
TTGATGAAGGTCTAAGGCAG+AGG 0.584118 2_4:-61765783 Msa0340410:CDS
ACGAGAGACAGTGTGGTCGT+TGG 0.590182 2_4:+61765702 None:intergenic
GTTTGCACACCGTACAAGTG+AGG 0.595543 2_4:-61764886 Msa0340410:CDS
TTGTGGTGGTGGTCGGTGAA+AGG 0.598501 2_4:+61765827 None:intergenic
TTTGCACACCGTACAAGTGA+GGG 0.621662 2_4:-61764885 Msa0340410:CDS
ATGAAGGTCTAAGGCAGAGG+TGG 0.630282 2_4:-61765780 Msa0340410:CDS
CACCATCGCCGCATAGACAG+CGG 0.661793 2_4:+61765872 None:intergenic
CACCACAATAGACAACAGGA+AGG 0.682027 2_4:-61765812 Msa0340410:CDS
GGCTAGCACCGCTGTCTATG+CGG 0.701078 2_4:-61765880 Msa0340410:intron
CTATGCGGCGATGGTGACGG+TGG 0.705728 2_4:-61765865 Msa0340410:CDS
CAAGCAGCAAGACATAAATG+CGG 0.729277 2_4:+61764816 None:intergenic
TGTGACGACGAGAGACAGTG+TGG 0.802230 2_4:+61765695 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTAAATTTATTAAGATT+GGG - chr2_4:61765387-61765406 Msa0340410:intron 10.0%
!! TTTGTAAATTTATTAAGATT+GGG - chr2_4:61765387-61765406 Msa0340410:intron 10.0%
!!! TAAACATTTCTATTCAATTT+TGG + chr2_4:61764884-61764903 None:intergenic 15.0%
!! AAAATTGAATAGAAATGTTT+AGG - chr2_4:61764883-61764902 Msa0340410:CDS 15.0%
!!! ATTATATAACCTGTATATTT+TGG - chr2_4:61765361-61765380 Msa0340410:intron 15.0%
!! GTTTGTAAATTTATTAAGAT+TGG - chr2_4:61765386-61765405 Msa0340410:intron 15.0%
!! TTGTAAATTTATTAAGATTG+GGG - chr2_4:61765388-61765407 Msa0340410:intron 15.0%
!!! TAAACATTTCTATTCAATTT+TGG + chr2_4:61764884-61764903 None:intergenic 15.0%
!! AAAATTGAATAGAAATGTTT+AGG - chr2_4:61764883-61764902 Msa0340410:CDS 15.0%
!!! ATTATATAACCTGTATATTT+TGG - chr2_4:61765361-61765380 Msa0340410:intron 15.0%
!! GTTTGTAAATTTATTAAGAT+TGG - chr2_4:61765386-61765405 Msa0340410:intron 15.0%
!! TTGTAAATTTATTAAGATTG+GGG - chr2_4:61765388-61765407 Msa0340410:intron 15.0%
!! TGTAAATTTATTAAGATTGG+GGG - chr2_4:61765389-61765408 Msa0340410:intron 20.0%
!!! TTTGCAATTTGATTTTTTTC+TGG - chr2_4:61765456-61765475 Msa0340410:intron 20.0%
!!! TTGCAATTTGATTTTTTTCT+GGG - chr2_4:61765457-61765476 Msa0340410:intron 20.0%
!! AATGTCTAGAAACTTATAAA+CGG + chr2_4:61765688-61765707 None:intergenic 20.0%
!! TGTAAATTTATTAAGATTGG+GGG - chr2_4:61765389-61765408 Msa0340410:intron 20.0%
!!! TTTGCAATTTGATTTTTTTC+TGG - chr2_4:61765456-61765475 Msa0340410:intron 20.0%
!!! TTGCAATTTGATTTTTTTCT+GGG - chr2_4:61765457-61765476 Msa0340410:intron 20.0%
!! AATGTCTAGAAACTTATAAA+CGG + chr2_4:61765688-61765707 None:intergenic 20.0%
! TACAAACTTCCAAAATATAC+AGG + chr2_4:61765373-61765392 None:intergenic 25.0%
!!! TGCAATTTGATTTTTTTCTG+GGG - chr2_4:61765458-61765477 Msa0340410:intron 25.0%
! AATGGAAATGAAATGATTAC+TGG - chr2_4:61765486-61765505 Msa0340410:intron 25.0%
! AAACTTATAAACGGTTATTG+TGG + chr2_4:61765679-61765698 None:intergenic 25.0%
!! TTTTTCTAGATTGAAGTCTT+TGG - chr2_4:61765716-61765735 Msa0340410:CDS 25.0%
!! TATGAGTTTAAGACATTGAT+AGG + chr2_4:61765792-61765811 None:intergenic 25.0%
! TACAAACTTCCAAAATATAC+AGG + chr2_4:61765373-61765392 None:intergenic 25.0%
!!! TGCAATTTGATTTTTTTCTG+GGG - chr2_4:61765458-61765477 Msa0340410:intron 25.0%
! AATGGAAATGAAATGATTAC+TGG - chr2_4:61765486-61765505 Msa0340410:intron 25.0%
! AAACTTATAAACGGTTATTG+TGG + chr2_4:61765679-61765698 None:intergenic 25.0%
!! TTTTTCTAGATTGAAGTCTT+TGG - chr2_4:61765716-61765735 Msa0340410:CDS 25.0%
!! TATGAGTTTAAGACATTGAT+AGG + chr2_4:61765792-61765811 None:intergenic 25.0%
!! TTTGGCTCTTTATGTCAAAA+TGG + chr2_4:61764866-61764885 None:intergenic 30.0%
CACAAATTAACACGCAAAAA+CGG + chr2_4:61765148-61765167 None:intergenic 30.0%
!!! TTTTGCGTGTTAATTTGTGT+TGG - chr2_4:61765149-61765168 Msa0340410:intron 30.0%
!! TTTGCGTGTTAATTTGTGTT+GGG - chr2_4:61765150-61765169 Msa0340410:intron 30.0%
!!! TTTTTTTCTGGGGTTAGAAA+TGG - chr2_4:61765468-61765487 Msa0340410:intron 30.0%
AAGTTGAACAATCATCAACT+TGG + chr2_4:61765574-61765593 None:intergenic 30.0%
TTAACCAATCTTGTTCTGTT+CGG + chr2_4:61765613-61765632 None:intergenic 30.0%
! AACAGAACAAGATTGGTTAA+TGG - chr2_4:61765613-61765632 Msa0340410:intron 30.0%
! GATTGGTTAATGGAACTTAT+TGG - chr2_4:61765623-61765642 Msa0340410:intron 30.0%
!! TTTGGCTCTTTATGTCAAAA+TGG + chr2_4:61764866-61764885 None:intergenic 30.0%
CACAAATTAACACGCAAAAA+CGG + chr2_4:61765148-61765167 None:intergenic 30.0%
!!! TTTTGCGTGTTAATTTGTGT+TGG - chr2_4:61765149-61765168 Msa0340410:intron 30.0%
!! TTTGCGTGTTAATTTGTGTT+GGG - chr2_4:61765150-61765169 Msa0340410:intron 30.0%
!!! TTTTTTTCTGGGGTTAGAAA+TGG - chr2_4:61765468-61765487 Msa0340410:intron 30.0%
AAGTTGAACAATCATCAACT+TGG + chr2_4:61765574-61765593 None:intergenic 30.0%
TTAACCAATCTTGTTCTGTT+CGG + chr2_4:61765613-61765632 None:intergenic 30.0%
! AACAGAACAAGATTGGTTAA+TGG - chr2_4:61765613-61765632 Msa0340410:intron 30.0%
! GATTGGTTAATGGAACTTAT+TGG - chr2_4:61765623-61765642 Msa0340410:intron 30.0%
GAAATGTTTAGGAAACCTAG+CGG - chr2_4:61764894-61764913 Msa0340410:CDS 35.0%
! AAGATGTTGATGAAGGTCTA+AGG - chr2_4:61765009-61765028 Msa0340410:intron 35.0%
! TAACACGCAAAAACGGTTTT+CGG + chr2_4:61765141-61765160 None:intergenic 35.0%
GGAAATCTCTTTCTATCTCA+AGG + chr2_4:61765553-61765572 None:intergenic 35.0%
TCTAAACTTATGACTGACAG+AGG - chr2_4:61765831-61765850 Msa0340410:CDS 35.0%
! AACTAGATGTTTTCTTTGCC+AGG - chr2_4:61765871-61765890 Msa0340410:CDS 35.0%
ATATTGAACCCTCACTTGTA+CGG + chr2_4:61765924-61765943 None:intergenic 35.0%
GTGAGGGTTCAATATCTTTA+TGG - chr2_4:61765929-61765948 Msa0340410:intron 35.0%
TAAATGCGGAACTAGAAAGA+TGG + chr2_4:61765971-61765990 None:intergenic 35.0%
GAAATGTTTAGGAAACCTAG+CGG - chr2_4:61764894-61764913 Msa0340410:CDS 35.0%
! AAGATGTTGATGAAGGTCTA+AGG - chr2_4:61765009-61765028 Msa0340410:intron 35.0%
! TAACACGCAAAAACGGTTTT+CGG + chr2_4:61765141-61765160 None:intergenic 35.0%
GGAAATCTCTTTCTATCTCA+AGG + chr2_4:61765553-61765572 None:intergenic 35.0%
TCTAAACTTATGACTGACAG+AGG - chr2_4:61765831-61765850 Msa0340410:CDS 35.0%
! AACTAGATGTTTTCTTTGCC+AGG - chr2_4:61765871-61765890 Msa0340410:CDS 35.0%
ATATTGAACCCTCACTTGTA+CGG + chr2_4:61765924-61765943 None:intergenic 35.0%
GTGAGGGTTCAATATCTTTA+TGG - chr2_4:61765929-61765948 Msa0340410:intron 35.0%
TAAATGCGGAACTAGAAAGA+TGG + chr2_4:61765971-61765990 None:intergenic 35.0%
TTCCTTCCTGTTGTCTATTG+TGG + chr2_4:61764991-61765010 None:intergenic 40.0%
! AGGAAGGAAGATGTTGATGA+AGG - chr2_4:61765002-61765021 Msa0340410:intron 40.0%
! AACACGCAAAAACGGTTTTC+GGG + chr2_4:61765140-61765159 None:intergenic 40.0%
CTGCATGTCAAATCTTGCTA+TGG + chr2_4:61765338-61765357 None:intergenic 40.0%
AGGAAAAACTGCTTTGTGTG+AGG + chr2_4:61765533-61765552 None:intergenic 40.0%
CAAGCAGCAAGACATAAATG+CGG + chr2_4:61765985-61766004 None:intergenic 40.0%
TTCCTTCCTGTTGTCTATTG+TGG + chr2_4:61764991-61765010 None:intergenic 40.0%
! AGGAAGGAAGATGTTGATGA+AGG - chr2_4:61765002-61765021 Msa0340410:intron 40.0%
! AACACGCAAAAACGGTTTTC+GGG + chr2_4:61765140-61765159 None:intergenic 40.0%
CTGCATGTCAAATCTTGCTA+TGG + chr2_4:61765338-61765357 None:intergenic 40.0%
AGGAAAAACTGCTTTGTGTG+AGG + chr2_4:61765533-61765552 None:intergenic 40.0%
CAAGCAGCAAGACATAAATG+CGG + chr2_4:61765985-61766004 None:intergenic 40.0%
ATGTTTAGGAAACCTAGCGG+CGG - chr2_4:61764897-61764916 Msa0340410:CDS 45.0%
!! TTGTCTATTGTGGTGGTGGT+CGG + chr2_4:61764981-61765000 None:intergenic 45.0%
CTTCCTGTTGTCTATTGTGG+TGG + chr2_4:61764988-61765007 None:intergenic 45.0%
CACCACAATAGACAACAGGA+AGG - chr2_4:61764986-61765005 Msa0340410:intron 45.0%
! TTGATGAAGGTCTAAGGCAG+AGG - chr2_4:61765015-61765034 Msa0340410:intron 45.0%
AACAGCGACAAAGCTCTGTT+TGG - chr2_4:61765053-61765072 Msa0340410:intron 45.0%
TTGGAGTTGAAAAGGCGATC+TGG + chr2_4:61765080-61765099 None:intergenic 45.0%
TGTGGTCGTTGGAGTTGAAA+AGG + chr2_4:61765088-61765107 None:intergenic 45.0%
CAATCTGCAATCTTCGTTCC+TGG + chr2_4:61765892-61765911 None:intergenic 45.0%
CAGGAACGAAGATTGCAGAT+TGG - chr2_4:61765890-61765909 Msa0340410:intron 45.0%
TTTGCACACCGTACAAGTGA+GGG - chr2_4:61765913-61765932 Msa0340410:intron 45.0%
ATGTTTAGGAAACCTAGCGG+CGG - chr2_4:61764897-61764916 Msa0340410:CDS 45.0%
!! TTGTCTATTGTGGTGGTGGT+CGG + chr2_4:61764981-61765000 None:intergenic 45.0%
CTTCCTGTTGTCTATTGTGG+TGG + chr2_4:61764988-61765007 None:intergenic 45.0%
CACCACAATAGACAACAGGA+AGG - chr2_4:61764986-61765005 Msa0340410:intron 45.0%
! TTGATGAAGGTCTAAGGCAG+AGG - chr2_4:61765015-61765034 Msa0340410:intron 45.0%
AACAGCGACAAAGCTCTGTT+TGG - chr2_4:61765053-61765072 Msa0340410:intron 45.0%
TTGGAGTTGAAAAGGCGATC+TGG + chr2_4:61765080-61765099 None:intergenic 45.0%
TGTGGTCGTTGGAGTTGAAA+AGG + chr2_4:61765088-61765107 None:intergenic 45.0%
CAATCTGCAATCTTCGTTCC+TGG + chr2_4:61765892-61765911 None:intergenic 45.0%
CAGGAACGAAGATTGCAGAT+TGG - chr2_4:61765890-61765909 Msa0340410:intron 45.0%
TTTGCACACCGTACAAGTGA+GGG - chr2_4:61765913-61765932 Msa0340410:intron 45.0%
CCTGTTGTCTATTGTGGTGG+TGG + chr2_4:61764985-61765004 None:intergenic 50.0%
CCACCACCACAATAGACAAC+AGG - chr2_4:61764982-61765001 Msa0340410:intron 50.0%
! ATGAAGGTCTAAGGCAGAGG+TGG - chr2_4:61765018-61765037 Msa0340410:intron 50.0%
TCTCTCGTCGTCACAGCAAA+TGG - chr2_4:61765111-61765130 Msa0340410:intron 50.0%
CTCTCGTCGTCACAGCAAAT+GGG - chr2_4:61765112-61765131 Msa0340410:intron 50.0%
GCGACCGAACAGAACAAGAT+TGG - chr2_4:61765606-61765625 Msa0340410:intron 50.0%
GTTTGCACACCGTACAAGTG+AGG - chr2_4:61765912-61765931 Msa0340410:intron 50.0%
CCTGTTGTCTATTGTGGTGG+TGG + chr2_4:61764985-61765004 None:intergenic 50.0%
CCACCACCACAATAGACAAC+AGG - chr2_4:61764982-61765001 Msa0340410:intron 50.0%
! ATGAAGGTCTAAGGCAGAGG+TGG - chr2_4:61765018-61765037 Msa0340410:intron 50.0%
TCTCTCGTCGTCACAGCAAA+TGG - chr2_4:61765111-61765130 Msa0340410:intron 50.0%
CTCTCGTCGTCACAGCAAAT+GGG - chr2_4:61765112-61765131 Msa0340410:intron 50.0%
GCGACCGAACAGAACAAGAT+TGG - chr2_4:61765606-61765625 Msa0340410:intron 50.0%
GTTTGCACACCGTACAAGTG+AGG - chr2_4:61765912-61765931 Msa0340410:intron 50.0%
TGTCTATGCGGCGATGGTGA+CGG - chr2_4:61764930-61764949 Msa0340410:intron 55.0%
!! TTGTGGTGGTGGTCGGTGAA+AGG + chr2_4:61764974-61764993 None:intergenic 55.0%
ACGAGAGACAGTGTGGTCGT+TGG + chr2_4:61765099-61765118 None:intergenic 55.0%
TGTGACGACGAGAGACAGTG+TGG + chr2_4:61765106-61765125 None:intergenic 55.0%
TGTCTATGCGGCGATGGTGA+CGG - chr2_4:61764930-61764949 Msa0340410:intron 55.0%
!! TTGTGGTGGTGGTCGGTGAA+AGG + chr2_4:61764974-61764993 None:intergenic 55.0%
ACGAGAGACAGTGTGGTCGT+TGG + chr2_4:61765099-61765118 None:intergenic 55.0%
TGTGACGACGAGAGACAGTG+TGG + chr2_4:61765106-61765125 None:intergenic 55.0%
GGCTAGCACCGCTGTCTATG+CGG - chr2_4:61764918-61764937 Msa0340410:intron 60.0%
CACCATCGCCGCATAGACAG+CGG + chr2_4:61764929-61764948 None:intergenic 60.0%
GGCTAGCACCGCTGTCTATG+CGG - chr2_4:61764918-61764937 Msa0340410:intron 60.0%
CACCATCGCCGCATAGACAG+CGG + chr2_4:61764929-61764948 None:intergenic 60.0%
CACCGCTGTCTATGCGGCGA+TGG - chr2_4:61764924-61764943 Msa0340410:intron 65.0%
!! CTATGCGGCGATGGTGACGG+TGG - chr2_4:61764933-61764952 Msa0340410:intron 65.0%
!! GCGATGGTGACGGTGGTGCT+AGG - chr2_4:61764940-61764959 Msa0340410:intron 65.0%
CACCGCTGTCTATGCGGCGA+TGG - chr2_4:61764924-61764943 Msa0340410:intron 65.0%
!! CTATGCGGCGATGGTGACGG+TGG - chr2_4:61764933-61764952 Msa0340410:intron 65.0%
!! GCGATGGTGACGGTGGTGCT+AGG - chr2_4:61764940-61764959 Msa0340410:intron 65.0%
! CAGCGGTGCTAGCCGCCGCT+AGG + chr2_4:61764912-61764931 None:intergenic 75.0%
! CAGCGGTGCTAGCCGCCGCT+AGG + chr2_4:61764912-61764931 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 61764815 61766005 61764815 ID=Msa0340410;Name=Msa0340410
chr2_4 mRNA 61764815 61766005 61764815 ID=Msa0340410-mRNA-1;Parent=Msa0340410;Name=Msa0340410-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0|0|0.66|0.5|0.66|3|0|114
chr2_4 exon 61765997 61766005 61765997 ID=Msa0340410-mRNA-1:exon:19534;Parent=Msa0340410-mRNA-1
chr2_4 exon 61765674 61765896 61765674 ID=Msa0340410-mRNA-1:exon:19533;Parent=Msa0340410-mRNA-1
chr2_4 exon 61764815 61764927 61764815 ID=Msa0340410-mRNA-1:exon:19532;Parent=Msa0340410-mRNA-1
chr2_4 CDS 61765997 61766005 61765997 ID=Msa0340410-mRNA-1:cds;Parent=Msa0340410-mRNA-1
chr2_4 CDS 61765674 61765896 61765674 ID=Msa0340410-mRNA-1:cds;Parent=Msa0340410-mRNA-1
chr2_4 CDS 61764815 61764927 61764815 ID=Msa0340410-mRNA-1:cds;Parent=Msa0340410-mRNA-1
Gene Sequence

>Msa0340410

ATGTTTTTGCACCGCTGTCTATGCGGCGATGGTGACGGTGGTGCTAGGTATTTTTACCTTTCACCGACCACCACCACAATAGACAACAGGAAGGAAGATGTTGATGAAGGTCTAAGGCAGAGGTGGTGTGAATTTGCGAACAGCGACAAAGCTCTGTTTGGACCAGATCGCCTTTTCAACTCCAACGACCACACTGTCTCTCGTCGTCACAGCAAATGGGTACCCGAAAACCGAACGAAGATTGCAGATTGGTTTGCACACCGTACAAGTGAGGGTTCAATATCTTTATGGTTTTGCTACAAATTCACCATCTTTCTAGTTCCGCATTTATGTCTTGCTGCTTGA

Protein sequence

>Msa0340410

MFLHRCLCGDGDGGARYFYLSPTTTTIDNRKEDVDEGLRQRWCEFANSDKALFGPDRLFNSNDHTVSRRHSKWVPENRTKIADWFAHRTSEGSISLWFCYKFTIFLVPHLCLAA*