AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0352410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0352410 MtrunA17_Chr1g0209681 77.372 137 7 2 1 114 1 136 1.30e-60 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0352410 AT1G57600.1 46.479 142 52 2 1 119 1 141 4.04e-35 126
Msa0352410 AT1G57600.2 46.479 142 52 2 1 119 1 141 4.04e-35 126
Msa0352410 AT1G57600.3 46.479 142 52 2 1 119 1 141 4.04e-35 126

Find 100 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAGGATCCTTCCTTTC+TGG 0.164123 3_1:+21220132 Msa0352410:three_prime_UTR
CAAGTAGATATTTATTTAAA+AGG 0.207136 3_1:+21220035 Msa0352410:three_prime_UTR
CTTTGGAATCTTTACACTTC+TGG 0.211962 3_1:+21218185 Msa0352410:CDS
TGGTTGGTCAACAAATTAAA+AGG 0.250958 3_1:+21218972 Msa0352410:three_prime_UTR
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 3_1:-21217760 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 3_1:+21217643 Msa0352410:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 3_1:+21218168 Msa0352410:CDS
GCTTAGTCTGAGATTAGTTT+TGG 0.281512 3_1:+21220555 Msa0352410:three_prime_UTR
TTCTTCTTCCTTCGCGAATT+CGG 0.299784 3_1:-21217087 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 3_1:-21220292 None:intergenic
ATAAAATTATAAGTCTAAAT+AGG 0.331713 3_1:-21220076 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 3_1:+21217019 Msa0352410:five_prime_UTR
AGGATCCTTCCTTTCTGGTT+AGG 0.351041 3_1:+21220137 Msa0352410:three_prime_UTR
AAAATGTAAGTAAAGGAGAT+AGG 0.351747 3_1:+21220503 Msa0352410:three_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 3_1:+21220830 Msa0352410:three_prime_UTR
GCATCTGAGGGGAAACTAAA+TGG 0.355710 3_1:+21218952 Msa0352410:three_prime_UTR
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 3_1:-21218208 None:intergenic
AATCTCAGACTAAGCTGAAT+TGG 0.361937 3_1:-21220547 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 3_1:-21217070 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 3_1:-21217013 None:intergenic
GAGAGATCAGACTTCTTAGT+TGG 0.380941 3_1:-21217567 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 3_1:+21217644 Msa0352410:five_prime_UTR
TACAAAATCATAGAATTATA+AGG 0.389269 3_1:-21220766 None:intergenic
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 3_1:-21217012 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 3_1:-21216842 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 3_1:-21217688 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 3_1:-21216843 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 3_1:-21216879 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 3_1:+21220849 Msa0352410:three_prime_UTR
ACAATTTAATGAGAAGTGAT+AGG 0.441583 3_1:+21220423 Msa0352410:three_prime_UTR
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 3_1:-21216850 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 3_1:+21218279 Msa0352410:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 3_1:-21217204 None:intergenic
ATGCTCCATGCAAATAAGAT+AGG 0.474131 3_1:-21218284 None:intergenic
TTGGGAGATCGAAGGAAGAC+AGG 0.474221 3_1:-21217548 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 3_1:+21218241 Msa0352410:CDS
AGAGATCAGACTTCTTAGTT+GGG 0.483449 3_1:-21217566 None:intergenic
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 3_1:-21216860 None:intergenic
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 3_1:-21216925 None:intergenic
AAATGTAAGTAAAGGAGATA+GGG 0.493237 3_1:+21220504 Msa0352410:three_prime_UTR
AATAGACTAAGTTGTGAGAC+TGG 0.497263 3_1:+21220578 Msa0352410:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 3_1:-21218168 None:intergenic
AGGAGCTAGGCTAAACCATC+AGG 0.501207 3_1:+21220443 Msa0352410:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 3_1:+21217606 Msa0352410:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 3_1:-21217759 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 3_1:-21220390 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.512641 3_1:-21216884 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 3_1:-21216998 None:intergenic
TTCTCATTAAATTGTCGAGA+TGG 0.524264 3_1:-21220415 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 3_1:+21218199 Msa0352410:CDS
TATTGATGAGAATATATGGC+AGG 0.526440 3_1:+21220096 Msa0352410:three_prime_UTR
CTCTTGCGATTCCACGATTG+GGG 0.530968 3_1:+21217645 Msa0352410:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 3_1:+21218225 Msa0352410:CDS
AAGAAATTCCACCTCCATCT+CGG 0.534799 3_1:+21220376 Msa0352410:three_prime_UTR
AAGAAAACGCATGTGAGCGT+TGG 0.536923 3_1:+21219990 Msa0352410:three_prime_UTR
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 3_1:-21216859 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 3_1:-21216849 None:intergenic
TTAAATTGCCGAGATGGAGG+TGG 0.549539 3_1:-21220384 None:intergenic
CTCTTAGCTTGGCAGTGCAT+AGG 0.552112 3_1:+21220724 Msa0352410:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 3_1:-21220458 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 3_1:+21218200 Msa0352410:CDS
ATAAATTGTTCGATTGAGAA+CGG 0.563409 3_1:-21216888 None:intergenic
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 3_1:-21216810 None:intergenic
CTGAGGGGAAACTAAATGGT+TGG 0.568184 3_1:+21218956 Msa0352410:three_prime_UTR
CAGTATCTGACAGCATCTGA+GGG 0.571126 3_1:+21218940 Msa0352410:exon
AATGAGAAGTGATAGGAGCT+AGG 0.574062 3_1:+21220430 Msa0352410:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 3_1:+21218286 Msa0352410:CDS
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 3_1:+21217169 Msa0352410:five_prime_UTR
AACAACCTAACCAGAAAGGA+AGG 0.580432 3_1:-21220142 None:intergenic
TCATTAAATTGCCGAGATGG+AGG 0.583358 3_1:-21220387 None:intergenic
ACAGTATCTGACAGCATCTG+AGG 0.584005 3_1:+21218939 Msa0352410:exon
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 3_1:-21217639 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 3_1:+21217599 Msa0352410:five_prime_UTR
TACTCCCATAGATTGTCTCA+AGG 0.588702 3_1:+21220287 Msa0352410:three_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 3_1:-21217656 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 3_1:-21216858 None:intergenic
ACTCCCATAGATTGTCTCAA+GGG 0.598235 3_1:+21220288 Msa0352410:three_prime_UTR
ATAAAACAACCTAACCAGAA+AGG 0.600525 3_1:-21220146 None:intergenic
CATCATCAGGCGCTCCCTCG+CGG 0.610543 3_1:+21217818 Msa0352410:CDS
CTTCTTAGTTGGGAGATCGA+AGG 0.610786 3_1:-21217556 None:intergenic
TCAAACCTGGAAAACGGACG+CGG 0.612446 3_1:+21217612 Msa0352410:five_prime_UTR
GGACTTTGCTAAGACTTGAG+AGG 0.614168 3_1:+21220117 Msa0352410:three_prime_UTR
ATAGGAGACTTGTGTTTCAG+CGG 0.616380 3_1:-21220058 None:intergenic
CTCCCATAGATTGTCTCAAG+GGG 0.617721 3_1:+21220289 Msa0352410:three_prime_UTR
GGCGAAAAGAACGAAGACAT+AGG 0.621764 3_1:+21220244 Msa0352410:intron
ATCCCCTTGAGACAATCTAT+GGG 0.622438 3_1:-21220291 None:intergenic
CAACAACAAACACAAGCTGT+CGG 0.626988 3_1:+21217144 Msa0352410:five_prime_UTR
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 3_1:+21218127 Msa0352410:CDS
AAAGGTAACCGAATTCGCGA+AGG 0.643245 3_1:+21217079 Msa0352410:five_prime_UTR
TGAATCGAACCAAACAGCAA+AGG 0.643380 3_1:+21217061 Msa0352410:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 3_1:-21216809 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 3_1:-21216934 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 3_1:-21216940 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 3_1:+21217716 Msa0352410:exon
AGAAAACAAAAGCCAGACAA+CGG 0.663004 3_1:-21218253 None:intergenic
AAAGAAGAAACATACCGCGA+GGG 0.665018 3_1:-21217832 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 3_1:-21218280 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 3_1:+21217742 Msa0352410:CDS
AGGATGTCTCAGATGCTCAG+TGG 0.679281 3_1:+21218124 Msa0352410:intron
AGTATCTGACAGCATCTGAG+GGG 0.720630 3_1:+21218941 Msa0352410:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAACTTAAATA+TGG - chr3_1:21219059-21219078 None:intergenic 10.0%
!! ATAAAATTATAAGTCTAAAT+AGG - chr3_1:21220079-21220098 None:intergenic 10.0%
!!! TGAAAAGTGAATTTTTTTTT+AGG - chr3_1:21216785-21216804 None:intergenic 15.0%
!!! ATTTGTTTTTTATTTATCGT+CGG + chr3_1:21217916-21217935 Msa0352410:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG + chr3_1:21218423-21218442 Msa0352410:CDS 15.0%
!! AAACAATGAAAATTAATCTT+TGG - chr3_1:21218787-21218806 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG + chr3_1:21218786-21218805 Msa0352410:intron 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG - chr3_1:21219160-21219179 None:intergenic 15.0%
!! GATAATAGATGAATAAATAA+AGG + chr3_1:21219846-21219865 Msa0352410:intron 15.0%
!! CAAGTAGATATTTATTTAAA+AGG + chr3_1:21220035-21220054 Msa0352410:three_prime_UTR 15.0%
!!! ATTTTATTGATGAGAATATA+TGG + chr3_1:21220092-21220111 Msa0352410:three_prime_UTR 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG + chr3_1:21220223-21220242 Msa0352410:intron 15.0%
!! TACAAAATCATAGAATTATA+AGG - chr3_1:21220769-21220788 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG + chr3_1:21220821-21220840 Msa0352410:three_prime_UTR 15.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG - chr3_1:21217331-21217350 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG + chr3_1:21218481-21218500 Msa0352410:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG + chr3_1:21218580-21218599 Msa0352410:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG - chr3_1:21218868-21218887 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG + chr3_1:21218867-21218886 Msa0352410:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG + chr3_1:21219193-21219212 Msa0352410:intron 20.0%
!!! ATACAATTATTCATTTGTTG+AGG - chr3_1:21219443-21219462 None:intergenic 20.0%
!! ATATGAATAGTATATGATGT+TGG + chr3_1:21219476-21219495 Msa0352410:intron 20.0%
!! TATGAATAGTATATGATGTT+GGG + chr3_1:21219477-21219496 Msa0352410:intron 20.0%
!!! GTTCTTTAATTTTTACAAGT+TGG + chr3_1:21219499-21219518 Msa0352410:intron 20.0%
!! AAAAACAATTATTATGACAC+CGG - chr3_1:21219619-21219638 None:intergenic 20.0%
!!! TTTTTTTTGTTGTGTAATGT+AGG + chr3_1:21219932-21219951 Msa0352410:intron 20.0%
!! AAATAACACACAAATTCATT+AGG - chr3_1:21220219-21220238 None:intergenic 20.0%
!! TGCAAAAAAAATGTAAGTAA+AGG + chr3_1:21220496-21220515 Msa0352410:three_prime_UTR 20.0%
!!! TTTTTTGTTTTGTTTTAGTG+TGG + chr3_1:21220652-21220671 Msa0352410:three_prime_UTR 20.0%
! ATAAATTGTTCGATTGAGAA+CGG - chr3_1:21216891-21216910 None:intergenic 25.0%
!!! TGATTTTGTTTCATAGATCT+AGG + chr3_1:21217238-21217257 Msa0352410:intron 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG + chr3_1:21217346-21217365 Msa0352410:intron 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG + chr3_1:21217512-21217531 Msa0352410:five_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG + chr3_1:21217805-21217824 Msa0352410:CDS 25.0%
! CTGAAATACTTACAAAGAAA+TGG - chr3_1:21218036-21218055 None:intergenic 25.0%
! CTTTACACATAACAAACAAT+AGG - chr3_1:21218106-21218125 None:intergenic 25.0%
!!! CTATTGTTTGTTATGTGTAA+AGG + chr3_1:21218104-21218123 Msa0352410:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG - chr3_1:21218683-21218702 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG - chr3_1:21218775-21218794 None:intergenic 25.0%
!! TTATTGACATGTTCTTCTTT+TGG + chr3_1:21218899-21218918 Msa0352410:intron 25.0%
!! AATATTTATCTTTCAAGTCC+TGG + chr3_1:21219025-21219044 Msa0352410:intron 25.0%
! ATAAAACTTAAATATGGCAC+AGG - chr3_1:21219053-21219072 None:intergenic 25.0%
! AGCATTATATATGCTCTAAT+TGG + chr3_1:21219408-21219427 Msa0352410:intron 25.0%
! TATTTGTGTTATATAGGTGT+CGG - chr3_1:21219673-21219692 None:intergenic 25.0%
! ATATGAAACTCTTGTATCTA+TGG - chr3_1:21219757-21219776 None:intergenic 25.0%
! TTGAGAAATGTTGATCAATT+AGG + chr3_1:21219780-21219799 Msa0352410:intron 25.0%
! TGAGAAATGTTGATCAATTA+GGG + chr3_1:21219781-21219800 Msa0352410:intron 25.0%
!!! TTTTTTACTATTTCAGCAAC+AGG - chr3_1:21219972-21219991 None:intergenic 25.0%
! ACAATTTAATGAGAAGTGAT+AGG + chr3_1:21220423-21220442 Msa0352410:three_prime_UTR 25.0%
! AAAATGTAAGTAAAGGAGAT+AGG + chr3_1:21220503-21220522 Msa0352410:three_prime_UTR 25.0%
! AAATGTAAGTAAAGGAGATA+GGG + chr3_1:21220504-21220523 Msa0352410:three_prime_UTR 25.0%
! AAACAAAAAAACTCAGAACA+AGG - chr3_1:21220642-21220661 None:intergenic 25.0%
!!! GGTTGTAGTTCTTTTATTTT+AGG + chr3_1:21220673-21220692 Msa0352410:three_prime_UTR 25.0%
! TATTGTAACTTTGGATTTGT+TGG + chr3_1:21220830-21220849 Msa0352410:three_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG - chr3_1:21217183-21217202 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG + chr3_1:21217295-21217314 Msa0352410:intron 30.0%
CTCGTTGGATAAATTTACAT+TGG + chr3_1:21217411-21217430 Msa0352410:intron 30.0%
CTACATAAACAGGTTATACA+TGG - chr3_1:21217865-21217884 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG - chr3_1:21217875-21217894 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG + chr3_1:21217944-21217963 Msa0352410:intron 30.0%
AATCATACATTGAGGTGATT+AGG - chr3_1:21217992-21218011 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG + chr3_1:21218225-21218244 Msa0352410:CDS 30.0%
TTATCTCTAACAGCAAGATA+AGG - chr3_1:21218705-21218724 None:intergenic 30.0%
TCTAAATTGAACAAGTGAGA+AGG - chr3_1:21218738-21218757 None:intergenic 30.0%
! TTATTGACACGTTCTTCTTT+TGG + chr3_1:21218818-21218837 Msa0352410:intron 30.0%
! TGGTTGGTCAACAAATTAAA+AGG + chr3_1:21218972-21218991 Msa0352410:three_prime_UTR 30.0%
TTCGAGTGACAAATGAAAAT+AGG - chr3_1:21219007-21219026 None:intergenic 30.0%
! CATGAATGTTTGGAATTACA+AGG - chr3_1:21219086-21219105 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG - chr3_1:21219096-21219115 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG + chr3_1:21219222-21219241 Msa0352410:intron 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG + chr3_1:21219223-21219242 Msa0352410:intron 30.0%
ATGCTAAATACACTTGAACA+CGG + chr3_1:21219273-21219292 Msa0352410:intron 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG + chr3_1:21219297-21219316 Msa0352410:intron 30.0%
CGGTTGTATTTGTGTTATAT+AGG - chr3_1:21219679-21219698 None:intergenic 30.0%
GAGAAATGTTGATCAATTAG+GGG + chr3_1:21219782-21219801 Msa0352410:intron 30.0%
TATTGATGAGAATATATGGC+AGG + chr3_1:21220096-21220115 Msa0352410:three_prime_UTR 30.0%
ATAAAACAACCTAACCAGAA+AGG - chr3_1:21220149-21220168 None:intergenic 30.0%
TTCTCATTAAATTGTCGAGA+TGG - chr3_1:21220418-21220437 None:intergenic 30.0%
! TTTTAGTCTAACTCTTAGCT+TGG + chr3_1:21220713-21220732 Msa0352410:three_prime_UTR 30.0%
TTGGATATTGTAACAATGAC+AGG + chr3_1:21220849-21220868 Msa0352410:three_prime_UTR 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG - chr3_1:21216816-21216835 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG - chr3_1:21216943-21216962 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG - chr3_1:21217001-21217020 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG + chr3_1:21217499-21217518 Msa0352410:intron 35.0%
AGAGATCAGACTTCTTAGTT+GGG - chr3_1:21217569-21217588 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG - chr3_1:21217691-21217710 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG - chr3_1:21218000-21218019 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG + chr3_1:21218185-21218204 Msa0352410:CDS 35.0%
AGAAAACAAAAGCCAGACAA+CGG - chr3_1:21218256-21218275 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG - chr3_1:21218283-21218302 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG - chr3_1:21218287-21218306 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG + chr3_1:21218286-21218305 Msa0352410:CDS 35.0%
! ACACGGATTTTGCATTTTAG+TGG + chr3_1:21219290-21219309 Msa0352410:intron 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG + chr3_1:21219298-21219317 Msa0352410:intron 35.0%
TAACACAAATACAACCGTGT+CGG + chr3_1:21219682-21219701 Msa0352410:intron 35.0%
TTCTCATTAAATTGCCGAGA+TGG - chr3_1:21220393-21220412 None:intergenic 35.0%
AATCTCAGACTAAGCTGAAT+TGG - chr3_1:21220550-21220569 None:intergenic 35.0%
!!! GCTTAGTCTGAGATTAGTTT+TGG + chr3_1:21220555-21220574 Msa0352410:three_prime_UTR 35.0%
AATAGACTAAGTTGTGAGAC+TGG + chr3_1:21220578-21220597 Msa0352410:three_prime_UTR 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG + chr3_1:21220585-21220604 Msa0352410:three_prime_UTR 35.0%
! GCATAGGCTATGTTTTACTT+TGG + chr3_1:21220740-21220759 Msa0352410:three_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG - chr3_1:21216817-21216836 None:intergenic 40.0%
GGGAAAGTGAAAAGGGAAAA+GGG - chr3_1:21216825-21216844 None:intergenic 40.0%
AGGGAAAGTGAAAAGGGAAA+AGG - chr3_1:21216826-21216845 None:intergenic 40.0%
GGGAAAAGGGAAAGTGAAAA+GGG - chr3_1:21216832-21216851 None:intergenic 40.0%
AGGGAAAAGGGAAAGTGAAA+AGG - chr3_1:21216833-21216852 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG - chr3_1:21216887-21216906 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG - chr3_1:21216928-21216947 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG - chr3_1:21216937-21216956 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG + chr3_1:21217061-21217080 Msa0352410:five_prime_UTR 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG - chr3_1:21217073-21217092 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG - chr3_1:21217090-21217109 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG + chr3_1:21217144-21217163 Msa0352410:five_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG + chr3_1:21217193-21217212 Msa0352410:five_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG - chr3_1:21217400-21217419 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG + chr3_1:21217490-21217509 Msa0352410:intron 40.0%
GAGAGATCAGACTTCTTAGT+TGG - chr3_1:21217570-21217589 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG + chr3_1:21217599-21217618 Msa0352410:five_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG + chr3_1:21217606-21217625 Msa0352410:five_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG + chr3_1:21217716-21217735 Msa0352410:exon 40.0%
AAGAACTCTTTCTGCTTCCA+GGG - chr3_1:21217762-21217781 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG - chr3_1:21217763-21217782 None:intergenic 40.0%
AAAGAAGAAACATACCGCGA+GGG - chr3_1:21217835-21217854 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG - chr3_1:21218171-21218190 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG + chr3_1:21218168-21218187 Msa0352410:CDS 40.0%
ACTTCTGGCCAATTTGATGA+GGG + chr3_1:21218200-21218219 Msa0352410:CDS 40.0%
GAAGAAAGCCCTCATCAAAT+TGG - chr3_1:21218211-21218230 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG + chr3_1:21218279-21218298 Msa0352410:CDS 40.0%
TATTTGCATGGAGCATGGTA+TGG + chr3_1:21218291-21218310 Msa0352410:intron 40.0%
TTAAATATGGCACAGGAACC+AGG - chr3_1:21219046-21219065 None:intergenic 40.0%
CGTTCCTTATCTATGAAGCA+CGG + chr3_1:21219527-21219546 Msa0352410:intron 40.0%
GTATCCGTGCTTCATAGATA+AGG - chr3_1:21219534-21219553 None:intergenic 40.0%
TACTTCGACACTCTTACGAT+CGG + chr3_1:21219644-21219663 Msa0352410:intron 40.0%
ATAGGAGACTTGTGTTTCAG+CGG - chr3_1:21220061-21220080 None:intergenic 40.0%
AACAACCTAACCAGAAAGGA+AGG - chr3_1:21220145-21220164 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG + chr3_1:21220287-21220306 Msa0352410:three_prime_UTR 40.0%
ACTCCCATAGATTGTCTCAA+GGG + chr3_1:21220288-21220307 Msa0352410:three_prime_UTR 40.0%
ATCCCCTTGAGACAATCTAT+GGG - chr3_1:21220294-21220313 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG - chr3_1:21220295-21220314 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG + chr3_1:21220376-21220395 Msa0352410:three_prime_UTR 40.0%
TCATTAAATTGCCGAGATGG+AGG - chr3_1:21220390-21220409 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG + chr3_1:21220430-21220449 Msa0352410:three_prime_UTR 40.0%
TCTGATGTATAAGCTCCTGA+TGG - chr3_1:21220461-21220480 None:intergenic 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG - chr3_1:21216845-21216864 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG - chr3_1:21216846-21216865 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG - chr3_1:21216862-21216881 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG - chr3_1:21216863-21216882 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG - chr3_1:21216882-21216901 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG - chr3_1:21217015-21217034 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG - chr3_1:21217016-21217035 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG + chr3_1:21217019-21217038 Msa0352410:five_prime_UTR 45.0%
AAAGGTAACCGAATTCGCGA+AGG + chr3_1:21217079-21217098 Msa0352410:five_prime_UTR 45.0%
CCTCTGTTCTTCCACGAAAA+CGG - chr3_1:21217207-21217226 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG - chr3_1:21217476-21217495 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG - chr3_1:21217488-21217507 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG + chr3_1:21217489-21217508 Msa0352410:intron 45.0%
CTTCTTAGTTGGGAGATCGA+AGG - chr3_1:21217559-21217578 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG + chr3_1:21217644-21217663 Msa0352410:five_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG + chr3_1:21217742-21217761 Msa0352410:CDS 45.0%
GAAAGAAGAAACATACCGCG+AGG - chr3_1:21217836-21217855 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG + chr3_1:21217955-21217974 Msa0352410:intron 45.0%
TGATTAGGTATGAGCCATCC+CGG - chr3_1:21217977-21217996 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG + chr3_1:21218199-21218218 Msa0352410:CDS 45.0%
ACAGTATCTGACAGCATCTG+AGG + chr3_1:21218939-21218958 Msa0352410:exon 45.0%
CAGTATCTGACAGCATCTGA+GGG + chr3_1:21218940-21218959 Msa0352410:exon 45.0%
AGTATCTGACAGCATCTGAG+GGG + chr3_1:21218941-21218960 Msa0352410:exon 45.0%
GCATCTGAGGGGAAACTAAA+TGG + chr3_1:21218952-21218971 Msa0352410:three_prime_UTR 45.0%
CTGAGGGGAAACTAAATGGT+TGG + chr3_1:21218956-21218975 Msa0352410:three_prime_UTR 45.0%
TATGAAGCACGGATACCTCT+AGG + chr3_1:21219538-21219557 Msa0352410:intron 45.0%
! CCGGTTCGACACATATCTTA+CGG - chr3_1:21219600-21219619 None:intergenic 45.0%
CCGTAAGATATGTGTCGAAC+CGG + chr3_1:21219597-21219616 Msa0352410:intron 45.0%
TTTGTCTGAGTTGTCCGACA+CGG - chr3_1:21219699-21219718 None:intergenic 45.0%
ACTCTTGTATCTATGGTGCG+CGG - chr3_1:21219750-21219769 None:intergenic 45.0%
AAGAAAACGCATGTGAGCGT+TGG + chr3_1:21219990-21220009 Msa0352410:three_prime_UTR 45.0%
GGACTTTGCTAAGACTTGAG+AGG + chr3_1:21220117-21220136 Msa0352410:three_prime_UTR 45.0%
TTGAGAGGATCCTTCCTTTC+TGG + chr3_1:21220132-21220151 Msa0352410:three_prime_UTR 45.0%
!! AGGATCCTTCCTTTCTGGTT+AGG + chr3_1:21220137-21220156 Msa0352410:three_prime_UTR 45.0%
GGCGAAAAGAACGAAGACAT+AGG + chr3_1:21220244-21220263 Msa0352410:intron 45.0%
CTCCCATAGATTGTCTCAAG+GGG + chr3_1:21220289-21220308 Msa0352410:three_prime_UTR 45.0%
TTAAATTGCCGAGATGGAGG+TGG - chr3_1:21220387-21220406 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG + chr3_1:21220591-21220610 Msa0352410:three_prime_UTR 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG + chr3_1:21220594-21220613 Msa0352410:three_prime_UTR 45.0%
!!! GGTTTTTGGAGTAGGTGGTT+CGG + chr3_1:21220599-21220618 Msa0352410:three_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG - chr3_1:21216812-21216831 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG - chr3_1:21216813-21216832 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG - chr3_1:21216852-21216871 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG - chr3_1:21216853-21216872 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG - chr3_1:21216861-21216880 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG + chr3_1:21217204-21217223 Msa0352410:five_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG + chr3_1:21217351-21217370 Msa0352410:intron 50.0%
GCCGGAATAGCAAATCTCGT+TGG + chr3_1:21217396-21217415 Msa0352410:intron 50.0%
TTGGGAGATCGAAGGAAGAC+AGG - chr3_1:21217551-21217570 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG + chr3_1:21217612-21217631 Msa0352410:five_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG - chr3_1:21217642-21217661 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG + chr3_1:21217643-21217662 Msa0352410:five_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG + chr3_1:21217645-21217664 Msa0352410:five_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG + chr3_1:21217956-21217975 Msa0352410:intron 50.0%
GATTAGGTATGAGCCATCCC+GGG - chr3_1:21217976-21217995 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG + chr3_1:21218124-21218143 Msa0352410:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG + chr3_1:21218127-21218146 Msa0352410:CDS 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG + chr3_1:21218138-21218157 Msa0352410:CDS 50.0%
TGAGAGGAATGTCCGTTGTC+TGG + chr3_1:21218241-21218260 Msa0352410:CDS 50.0%
!! TTACGGTGTCATACGCGTGT+CGG - chr3_1:21219583-21219602 None:intergenic 50.0%
AGGAGCTAGGCTAAACCATC+AGG + chr3_1:21220443-21220462 Msa0352410:three_prime_UTR 50.0%
! CTCTTAGCTTGGCAGTGCAT+AGG + chr3_1:21220724-21220743 Msa0352410:three_prime_UTR 50.0%
GCAGCTCGAATCCGAAGAAG+AGG + chr3_1:21217169-21217188 Msa0352410:five_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG - chr3_1:21217392-21217411 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG - chr3_1:21217395-21217414 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG - chr3_1:21217659-21217678 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG + chr3_1:21217960-21217979 Msa0352410:intron 55.0%
!! GCACGGATACCTCTAGGACT+AGG + chr3_1:21219544-21219563 Msa0352410:intron 55.0%
!! CTCTAGGACTAGGCATGTCG+CGG + chr3_1:21219554-21219573 Msa0352410:intron 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG + chr3_1:21217354-21217373 Msa0352410:intron 60.0%
! GAACACCGCGTCCGTTTTCC+AGG - chr3_1:21217620-21217639 None:intergenic 60.0%
CGCGACATGCCTAGTCCTAG+AGG - chr3_1:21219556-21219575 None:intergenic 60.0%
TGCTATTCCGGCGAGGTGGC+CGG - chr3_1:21217388-21217407 None:intergenic 65.0%
CATCATCAGGCGCTCCCTCG+CGG + chr3_1:21217818-21217837 Msa0352410:CDS 65.0%
TATTCCGGCGAGGTGGCCGG+TGG - chr3_1:21217385-21217404 None:intergenic 70.0%
!! CGGTGGTGGCACCACCGCGC+CGG - chr3_1:21217368-21217387 None:intergenic 80.0%
!!! CGGCGCGGTGGTGCCACCAC+CGG + chr3_1:21217366-21217385 Msa0352410:intron 80.0%
! GCCACCACCGGCCACCTCGC+CGG + chr3_1:21217378-21217397 Msa0352410:intron 80.0%
! TCCGGCGAGGTGGCCGGTGG+TGG - chr3_1:21217382-21217401 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 21216772 21220916 21216772 ID=Msa0352410;Name=Msa0352410
chr3_1 mRNA 21216772 21220916 21216772 ID=Msa0352410-mRNA-1;Parent=Msa0352410;Name=Msa0352410-mRNA-1;_AED=0.22;_eAED=0.22;_QI=655|1|1|1|0.42|0.25|8|921|120
chr3_1 exon 21216772 21216818 21216772 ID=Msa0352410-mRNA-1:exon:4820;Parent=Msa0352410-mRNA-1
chr3_1 exon 21216839 21217225 21216839 ID=Msa0352410-mRNA-1:exon:4821;Parent=Msa0352410-mRNA-1
chr3_1 exon 21217508 21217839 21217508 ID=Msa0352410-mRNA-1:exon:4822;Parent=Msa0352410-mRNA-1
chr3_1 exon 21218126 21218307 21218126 ID=Msa0352410-mRNA-1:exon:4823;Parent=Msa0352410-mRNA-1
chr3_1 exon 21218396 21218444 21218396 ID=Msa0352410-mRNA-1:exon:4824;Parent=Msa0352410-mRNA-1
chr3_1 exon 21218929 21218993 21218929 ID=Msa0352410-mRNA-1:exon:4825;Parent=Msa0352410-mRNA-1
chr3_1 exon 21219954 21220158 21219954 ID=Msa0352410-mRNA-1:exon:4826;Parent=Msa0352410-mRNA-1
chr3_1 exon 21220245 21220916 21220245 ID=Msa0352410-mRNA-1:exon:4827;Parent=Msa0352410-mRNA-1
chr3_1 five_prime_UTR 21216772 21216818 21216772 ID=Msa0352410-mRNA-1:five_prime_utr;Parent=Msa0352410-mRNA-1
chr3_1 five_prime_UTR 21216839 21217225 21216839 ID=Msa0352410-mRNA-1:five_prime_utr;Parent=Msa0352410-mRNA-1
chr3_1 five_prime_UTR 21217508 21217728 21217508 ID=Msa0352410-mRNA-1:five_prime_utr;Parent=Msa0352410-mRNA-1
chr3_1 CDS 21217729 21217839 21217729 ID=Msa0352410-mRNA-1:cds;Parent=Msa0352410-mRNA-1
chr3_1 CDS 21218126 21218307 21218126 ID=Msa0352410-mRNA-1:cds;Parent=Msa0352410-mRNA-1
chr3_1 CDS 21218396 21218444 21218396 ID=Msa0352410-mRNA-1:cds;Parent=Msa0352410-mRNA-1
chr3_1 CDS 21218929 21218949 21218929 ID=Msa0352410-mRNA-1:cds;Parent=Msa0352410-mRNA-1
chr3_1 three_prime_UTR 21218950 21218993 21218950 ID=Msa0352410-mRNA-1:three_prime_utr;Parent=Msa0352410-mRNA-1
chr3_1 three_prime_UTR 21219954 21220158 21219954 ID=Msa0352410-mRNA-1:three_prime_utr;Parent=Msa0352410-mRNA-1
chr3_1 three_prime_UTR 21220245 21220916 21220245 ID=Msa0352410-mRNA-1:three_prime_utr;Parent=Msa0352410-mRNA-1
Gene Sequence

>Msa0352410

ATGAACAAGGACAACTATAAGAAGAGTGCACCCTGGAAGCAGAAAGAGTTCTTTTTTCTCGTCACTTATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGTCATTGTCGAACAGTATCTGA
Protein sequence

>Msa0352410

MNKDNYKKSAPWKQKEFFFLVTYAFIFYVIIIRRSLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKSLSNSI*