AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1112290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1112290 MtrunA17_Chr1g0209681 77.372 137 7 2 1 114 1 136 2.90e-60 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1112290 AT1G57600.1 46.479 142 52 2 1 119 1 141 7.83e-35 126
Msa1112290 AT1G57600.2 46.479 142 52 2 1 119 1 141 7.83e-35 126
Msa1112290 AT1G57600.3 46.479 142 52 2 1 119 1 141 7.83e-35 126

Find 103 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTGTGTGTTATTTATTT+AGG 0.200548 7_3:-58248469 Msa1112290:three_prime_UTR
CTTTGGAATCTTTACACTTC+TGG 0.211962 7_3:-58249774 Msa1112290:CDS
TGGTTGGTCAACAAATTAAA+AGG 0.250958 7_3:-58248987 Msa1112290:intron
AAAGAACTCTTTCTGCTTCC+AGG 0.261759 7_3:+58250201 None:intergenic
CTCTCTTGCGATTCCACGAT+TGG 0.265352 7_3:-58250318 Msa1112290:five_prime_UTR
CCTGTTCTTACACTTGTCTT+TGG 0.273288 7_3:-58249791 Msa1112290:CDS
GCTTAGTCTGAGATTAGTTT+TGG 0.281512 7_3:-58248137 Msa1112290:three_prime_UTR
TTCTTCTTCCTTCGCGAATT+CGG 0.299784 7_3:+58250874 None:intergenic
TATCCCCTTGAGACAATCTA+TGG 0.313160 7_3:+58248400 None:intergenic
CAGAAGCACAAAGCTGAGTT+TGG 0.350409 7_3:-58250942 Msa1112290:five_prime_UTR
AAAATGTAAGTAAAGGAGAT+AGG 0.351747 7_3:-58248189 Msa1112290:three_prime_UTR
TATTGTAACTTTGGATTTGT+TGG 0.353906 7_3:-58247862 Msa1112290:three_prime_UTR
GCATCTGAGGGGAAACTAAA+TGG 0.355710 7_3:-58249007 Msa1112290:exon
GAAGAAAGCCCTCATCAAAT+TGG 0.359772 7_3:+58249751 None:intergenic
AATCTCAGACTAAGCTGAAT+TGG 0.361937 7_3:+58248145 None:intergenic
ATTCGGTTACCTTTGCTGTT+TGG 0.369402 7_3:+58250891 None:intergenic
TCAGCTTTGTGCTTCTGACT+TGG 0.372127 7_3:+58250948 None:intergenic
GAAAAGGGAAAAGGGAAAAG+TGG 0.376759 7_3:+58251147 None:intergenic
GAGAGATCAGACTTCTTAGT+TGG 0.380941 7_3:+58250394 None:intergenic
TCTCTTGCGATTCCACGATT+GGG 0.383180 7_3:-58250317 Msa1112290:five_prime_UTR
TACAAAATCATAGAATTATA+AGG 0.389269 7_3:+58247926 None:intergenic
CAGCTTTGTGCTTCTGACTT+GGG 0.392786 7_3:+58250949 None:intergenic
GAAGGGGAGAAAAGGGAAAA+GGG 0.395097 7_3:+58251119 None:intergenic
AAATAACACACAAATTCATT+AGG 0.412866 7_3:+58248476 None:intergenic
CTAATCAGCAACTCAATTCA+AGG 0.416062 7_3:+58250273 None:intergenic
TGAAGGGGAGAAAAGGGAAA+AGG 0.420446 7_3:+58251118 None:intergenic
TCGATTGAGAACGGTTGGTT+TGG 0.423465 7_3:+58251082 None:intergenic
AGGGAAAGTGAAAAGGGAAA+AGG 0.433908 7_3:+58251138 None:intergenic
TTGGATATTGTAACAATGAC+AGG 0.433916 7_3:-58247843 Msa1112290:three_prime_UTR
ACAATTTAATGAGAAGTGAT+AGG 0.441583 7_3:-58248269 Msa1112290:three_prime_UTR
AGGGAAAAGGGAAAGTGAAA+AGG 0.448159 7_3:+58251131 None:intergenic
GGGAAAGTGAAAAGGGAAAA+GGG 0.449050 7_3:+58251139 None:intergenic
TCACGAGTGAAGGGGAGAAA+AGG 0.466417 7_3:+58251111 None:intergenic
GGGAAAAGGGAAAGTGAAAA+GGG 0.467752 7_3:+58251132 None:intergenic
GCCTACCTATCTTATTTGCA+TGG 0.471564 7_3:-58249680 Msa1112290:CDS
CCTCTGTTCTTCCACGAAAA+CGG 0.471630 7_3:+58250757 None:intergenic
AAGTATCTGAACTTTGAAGT+AGG 0.473094 7_3:-58248737 Msa1112290:three_prime_UTR
ATGCTCCATGCAAATAAGAT+AGG 0.474131 7_3:+58249675 None:intergenic
TTGGGAGATCGAAGGAAGAC+AGG 0.474221 7_3:+58250413 None:intergenic
TGAGAGGAATGTCCGTTGTC+TGG 0.481624 7_3:-58249718 Msa1112290:CDS
AGAGATCAGACTTCTTAGTT+GGG 0.483449 7_3:+58250395 None:intergenic
TTGGAGAGTTTCACGAGTGA+AGG 0.487075 7_3:+58251101 None:intergenic
AGACTTGGTGAAGGTTGAAT+CGG 0.487863 7_3:+58251036 None:intergenic
AAATGTAAGTAAAGGAGATA+GGG 0.493237 7_3:-58248188 Msa1112290:three_prime_UTR
AATAGACTAAGTTGTGAGAC+TGG 0.497263 7_3:-58248114 Msa1112290:three_prime_UTR
CCAAAGACAAGTGTAAGAAC+AGG 0.498674 7_3:+58249791 None:intergenic
AGTATCTGAACTTTGAAGTA+GGG 0.499224 7_3:-58248736 Msa1112290:three_prime_UTR
AGGAGCTAGGCTAAACCATC+AGG 0.501207 7_3:-58248249 Msa1112290:three_prime_UTR
CTGTACTCAAACCTGGAAAA+CGG 0.505341 7_3:-58250355 Msa1112290:five_prime_UTR
AAGAACTCTTTCTGCTTCCA+GGG 0.506003 7_3:+58250202 None:intergenic
TTCTCATTAAATTGCCGAGA+TGG 0.510011 7_3:+58248302 None:intergenic
ATTGTTCGATTGAGAACGGT+TGG 0.512641 7_3:+58251077 None:intergenic
AAAAGGGAAAAGGGAAAAGT+GGG 0.520337 7_3:+58251148 None:intergenic
TGACTTGGGTTTGATATGAA+CGG 0.521217 7_3:+58250963 None:intergenic
TTCTCATTAAATTGTCGAGA+TGG 0.524264 7_3:+58248277 None:intergenic
CACTTCTGGCCAATTTGATG+AGG 0.526184 7_3:-58249760 Msa1112290:CDS
CTCTTGCGATTCCACGATTG+GGG 0.530968 7_3:-58250316 Msa1112290:five_prime_UTR
TTCTTCAATTTCAATGTGAG+AGG 0.534160 7_3:-58249734 Msa1112290:CDS
AACATAGCCTATGCACTGCC+AGG 0.534676 7_3:+58247961 None:intergenic
AAGAAATTCCACCTCCATCT+CGG 0.534799 7_3:-58248316 Msa1112290:three_prime_UTR
TGGAGAGTTTCACGAGTGAA+GGG 0.546915 7_3:+58251102 None:intergenic
CACGAGTGAAGGGGAGAAAA+GGG 0.548165 7_3:+58251112 None:intergenic
TTAAATTGCCGAGATGGAGG+TGG 0.549539 7_3:+58248308 None:intergenic
CTCTTAGCCTGGCAGTGCAT+AGG 0.552112 7_3:-58247968 Msa1112290:three_prime_UTR
TCTGATGTATAAGCTCCTGA+TGG 0.558906 7_3:+58248234 None:intergenic
ACTTCTGGCCAATTTGATGA+GGG 0.561165 7_3:-58249759 Msa1112290:CDS
ATAAATTGTTCGATTGAGAA+CGG 0.563409 7_3:+58251073 None:intergenic
AGGGAAAAGGGAAAAGTGGG+AGG 0.567293 7_3:+58251151 None:intergenic
CTGAGGGGAAACTAAATGGT+TGG 0.568184 7_3:-58249003 Msa1112290:exon
ACAGTATCCGACAGCATCTG+AGG 0.568896 7_3:-58249020 Msa1112290:CDS
AATGAGAAGTGATAGGAGCT+AGG 0.574062 7_3:-58248262 Msa1112290:three_prime_UTR
TATCTTATTTGCATGGAGCA+TGG 0.574992 7_3:-58249673 Msa1112290:intron
GCAGCTCGAATCCGAAGAAG+AGG 0.577668 7_3:-58250792 Msa1112290:five_prime_UTR
TCATTAAATTGCCGAGATGG+AGG 0.583358 7_3:+58248305 None:intergenic
TCGTGGAATCGCAAGAGAGA+AGG 0.584250 7_3:+58250322 None:intergenic
TCAACAACTGTACTCAAACC+TGG 0.585004 7_3:-58250362 Msa1112290:five_prime_UTR
TACTCCCATAGATTGTCTCA+AGG 0.588702 7_3:-58248405 Msa1112290:three_prime_UTR
TGTGAATAGCGCCCCAATCG+TGG 0.589285 7_3:+58250305 None:intergenic
CAGTATCCGACAGCATCTGA+GGG 0.590060 7_3:-58249019 Msa1112290:CDS
AGTTTCCCCTCAGATGCTGT+CGG 0.590938 7_3:+58249013 None:intergenic
GGAGAGTTTCACGAGTGAAG+GGG 0.598071 7_3:+58251103 None:intergenic
ACTCCCATAGATTGTCTCAA+GGG 0.598235 7_3:-58248404 Msa1112290:three_prime_UTR
CATCATCAGGCGCTCCCTCG+CGG 0.610543 7_3:-58250143 Msa1112290:intron
CTTCTTAGTTGGGAGATCGA+AGG 0.610786 7_3:+58250405 None:intergenic
TCAAACCTGGAAAACGGACG+CGG 0.612446 7_3:-58250349 Msa1112290:five_prime_UTR
CTCCCATAGATTGTCTCAAG+GGG 0.617721 7_3:-58248403 Msa1112290:three_prime_UTR
GGCGAAAAGAACGAAGACAT+AGG 0.621764 7_3:-58248448 Msa1112290:three_prime_UTR
ATCCCCTTGAGACAATCTAT+GGG 0.622438 7_3:+58248401 None:intergenic
CAACAACAAACACAAGCTGT+CGG 0.626988 7_3:-58250817 Msa1112290:five_prime_UTR
ATGTCTCAGATGCTCAGTGG+AGG 0.637896 7_3:-58249832 Msa1112290:CDS
AAAGGTAACCGAATTCGCGA+AGG 0.643245 7_3:-58250882 Msa1112290:five_prime_UTR
TGAATCGAACCAAACAGCAA+AGG 0.643380 7_3:-58250900 Msa1112290:five_prime_UTR
GGGAAAAGGGAAAAGTGGGA+GGG 0.644660 7_3:+58251152 None:intergenic
GTTGAAATCAGACTTGGTGA+AGG 0.644985 7_3:+58251027 None:intergenic
GATGTTGTTGAAATCAGACT+TGG 0.654774 7_3:+58251021 None:intergenic
ACAGTACATTGCGATGAACA+AGG 0.655075 7_3:-58250245 Msa1112290:exon
AGAAAACAAAAGCCAGACAA+CGG 0.663004 7_3:+58249706 None:intergenic
AAAGAAGAAACATACCGCGA+GGG 0.665018 7_3:+58250129 None:intergenic
TCCATGCAAATAAGATAGGT+AGG 0.665647 7_3:+58249679 None:intergenic
ACTATAAGAAGAGTGCACCC+TGG 0.671406 7_3:-58250219 Msa1112290:CDS
AGGATGTCTCAGATGCTCAG+TGG 0.679281 7_3:-58249835 Msa1112290:CDS
ATGCTAAATACACTTGAACA+CGG 0.697896 7_3:-58248686 Msa1112290:three_prime_UTR
AGTATCCGACAGCATCTGAG+GGG 0.723113 7_3:-58249018 Msa1112290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAACTTAAATA+TGG + chr7_3:58250098-58250117 None:intergenic 10.0%
!!! ATTTGTTTTTTATTTATCGT+CGG - chr7_3:58248953-58248972 Msa1112290:intron 15.0%
!!! TATTAATTTTCTTCTTGTTA+AGG - chr7_3:58249462-58249481 Msa1112290:intron 15.0%
!! AAACAATGAAAATTAATCTT+TGG + chr7_3:58249826-58249845 None:intergenic 15.0%
!!! AAAGATTAATTTTCATTGTT+TGG - chr7_3:58249825-58249844 Msa1112290:CDS 15.0%
!!! ATTTTAAAACAAGTTTTAAG+TGG + chr7_3:58250199-58250218 None:intergenic 15.0%
!!! AATTTGTGTGTTATTTATTT+AGG - chr7_3:58250529-58250548 Msa1112290:intron 15.0%
!! TACAAAATCATAGAATTATA+AGG + chr7_3:58251075-58251094 None:intergenic 15.0%
!!! ATTTTATGTTATTGTAACTT+TGG - chr7_3:58251127-58251146 Msa1112290:five_prime_UTR 15.0%
!!! TGATTTTGTTTCATAGATTT+AGG - chr7_3:58248275-58248294 Msa1112290:three_prime_UTR 20.0%
!!! ATTTTCTTCAAAGTTTTTCA+CGG + chr7_3:58248368-58248387 None:intergenic 20.0%
!!! AATATTTCTATGTAATGTGT+TGG - chr7_3:58249520-58249539 Msa1112290:intron 20.0%
!! GATATAGAAATCTAGTTAAA+AGG - chr7_3:58249619-58249638 Msa1112290:intron 20.0%
!! AAACAATGAAAATTCAACTA+TGG + chr7_3:58249907-58249926 None:intergenic 20.0%
!!! ATAGTTGAATTTTCATTGTT+TGG - chr7_3:58249906-58249925 Msa1112290:intron 20.0%
!!! ATTAGAAACTTATTTTGTCA+TGG - chr7_3:58250232-58250251 Msa1112290:CDS 20.0%
!! AAATAACACACAAATTCATT+AGG + chr7_3:58250525-58250544 None:intergenic 20.0%
!! TGCAAAAAAAATGTAAGTAA+AGG - chr7_3:58250802-58250821 Msa1112290:five_prime_UTR 20.0%
! ATAAATTGTTCGATTGAGAA+CGG + chr7_3:58247928-58247947 None:intergenic 25.0%
!! AAAATAAGAGTTTGAAACTC+CGG - chr7_3:58248383-58248402 Msa1112290:three_prime_UTR 25.0%
! GCAAAAAAGGAAGAAAAAAA+TGG - chr7_3:58248549-58248568 Msa1112290:three_prime_UTR 25.0%
!! TTTTCTATGTTATCATCATC+AGG - chr7_3:58248842-58248861 Msa1112290:intron 25.0%
! CTGAAATACTTACAAAGAAA+TGG + chr7_3:58249073-58249092 None:intergenic 25.0%
! TTACACATAACAAACAGAAT+AGG + chr7_3:58249143-58249162 None:intergenic 25.0%
!!! ATTCTGTTTGTTATGTGTAA+AGG - chr7_3:58249143-58249162 Msa1112290:intron 25.0%
! GTTTGTGACAAAAATAACAT+TGG + chr7_3:58249722-58249741 None:intergenic 25.0%
! TTAATCTTTGGCATTTGTTT+CGG + chr7_3:58249814-58249833 None:intergenic 25.0%
!! TTATTGACATGTTCTTCTTT+TGG - chr7_3:58249938-58249957 Msa1112290:intron 25.0%
!! AATATTTATCTTTCAAGTCC+TGG - chr7_3:58250064-58250083 Msa1112290:intron 25.0%
! ATAAAACTTAAATATGGCAC+AGG + chr7_3:58250092-58250111 None:intergenic 25.0%
! ACAATTTAATGAGAAGTGAT+AGG - chr7_3:58250729-58250748 Msa1112290:intron 25.0%
! AAAATGTAAGTAAAGGAGAT+AGG - chr7_3:58250809-58250828 Msa1112290:five_prime_UTR 25.0%
! AAATGTAAGTAAAGGAGATA+GGG - chr7_3:58250810-58250829 Msa1112290:five_prime_UTR 25.0%
! AAACAAAAAAACTCAGAACA+AGG + chr7_3:58250948-58250967 None:intergenic 25.0%
!!! GCTTGTAGTTCTTTTATTTT+AGG - chr7_3:58250979-58250998 Msa1112290:five_prime_UTR 25.0%
! TATTGTAACTTTGGATTTGT+TGG - chr7_3:58251136-58251155 Msa1112290:five_prime_UTR 25.0%
! TCTATTCTTTTCCTCTTCTT+CGG + chr7_3:58248220-58248239 None:intergenic 30.0%
GAAATGAGTTTAGAAAGTGA+AGG - chr7_3:58248332-58248351 Msa1112290:three_prime_UTR 30.0%
CTCGTTGGATAAATTTACAT+TGG - chr7_3:58248448-58248467 Msa1112290:three_prime_UTR 30.0%
CTACATAAACAGGTTATACA+TGG + chr7_3:58248902-58248921 None:intergenic 30.0%
GTCATATCATCTACATAAAC+AGG + chr7_3:58248912-58248931 None:intergenic 30.0%
GATCACTACAAACAACTATT+CGG - chr7_3:58248981-58249000 Msa1112290:intron 30.0%
AATCATACATTGAGGTGATT+AGG + chr7_3:58249029-58249048 None:intergenic 30.0%
TTCTTCAATTTCAATGTGAG+AGG - chr7_3:58249264-58249283 Msa1112290:intron 30.0%
TTATCTCTAACAGCAAGATA+AGG + chr7_3:58249744-58249763 None:intergenic 30.0%
TCTAAATTGAACAAGTGAGA+AGG + chr7_3:58249777-58249796 None:intergenic 30.0%
! TTATTGACACGTTCTTCTTT+TGG - chr7_3:58249857-58249876 Msa1112290:intron 30.0%
! TGGTTGGTCAACAAATTAAA+AGG - chr7_3:58250011-58250030 Msa1112290:intron 30.0%
TTCGAGTGACAAATGAAAAT+AGG + chr7_3:58250046-58250065 None:intergenic 30.0%
! CATGAATGTTTGGAATTACA+AGG + chr7_3:58250125-58250144 None:intergenic 30.0%
TGACAACTTTCATGAATGTT+TGG + chr7_3:58250135-58250154 None:intergenic 30.0%
!! AAGTATCTGAACTTTGAAGT+AGG - chr7_3:58250261-58250280 Msa1112290:five_prime_UTR 30.0%
!! AGTATCTGAACTTTGAAGTA+GGG - chr7_3:58250262-58250281 Msa1112290:five_prime_UTR 30.0%
ATGCTAAATACACTTGAACA+CGG - chr7_3:58250312-58250331 Msa1112290:five_prime_UTR 30.0%
!! TTTTGCATTTTAGTGGAACA+CGG - chr7_3:58250336-58250355 Msa1112290:five_prime_UTR 30.0%
TTCTCATTAAATTGTCGAGA+TGG + chr7_3:58250724-58250743 None:intergenic 30.0%
TTGGATATTGTAACAATGAC+AGG - chr7_3:58251155-58251174 Msa1112290:five_prime_UTR 30.0%
AAAAGGGAAAAGGGAAAAGT+GGG + chr7_3:58247853-58247872 None:intergenic 35.0%
GATGTTGTTGAAATCAGACT+TGG + chr7_3:58247980-58247999 None:intergenic 35.0%
! TGACTTGGGTTTGATATGAA+CGG + chr7_3:58248038-58248057 None:intergenic 35.0%
AAACAACAGAAGGGCAAAAA+AGG - chr7_3:58248536-58248555 Msa1112290:three_prime_UTR 35.0%
AGAGATCAGACTTCTTAGTT+GGG + chr7_3:58248606-58248625 None:intergenic 35.0%
CTAATCAGCAACTCAATTCA+AGG + chr7_3:58248728-58248747 None:intergenic 35.0%
AAGAGCAGAATCATACATTG+AGG + chr7_3:58249037-58249056 None:intergenic 35.0%
!! CTTTGGAATCTTTACACTTC+TGG - chr7_3:58249224-58249243 Msa1112290:intron 35.0%
AGAAAACAAAAGCCAGACAA+CGG + chr7_3:58249295-58249314 None:intergenic 35.0%
TCCATGCAAATAAGATAGGT+AGG + chr7_3:58249322-58249341 None:intergenic 35.0%
ATGCTCCATGCAAATAAGAT+AGG + chr7_3:58249326-58249345 None:intergenic 35.0%
! TATCTTATTTGCATGGAGCA+TGG - chr7_3:58249325-58249344 Msa1112290:intron 35.0%
! ACACGGATTTTGCATTTTAG+TGG - chr7_3:58250329-58250348 Msa1112290:five_prime_UTR 35.0%
!! TTTGCATTTTAGTGGAACAC+GGG - chr7_3:58250337-58250356 Msa1112290:five_prime_UTR 35.0%
TTCTCATTAAATTGCCGAGA+TGG + chr7_3:58250699-58250718 None:intergenic 35.0%
AATCTCAGACTAAGCTGAAT+TGG + chr7_3:58250856-58250875 None:intergenic 35.0%
!!! GCTTAGTCTGAGATTAGTTT+TGG - chr7_3:58250861-58250880 Msa1112290:five_prime_UTR 35.0%
AATAGACTAAGTTGTGAGAC+TGG - chr7_3:58250884-58250903 Msa1112290:five_prime_UTR 35.0%
!!! TAAGTTGTGAGACTGGTTTT+TGG - chr7_3:58250891-58250910 Msa1112290:five_prime_UTR 35.0%
! TTTTAGTCTAACTCTTAGCC+TGG - chr7_3:58251019-58251038 Msa1112290:five_prime_UTR 35.0%
! GCATAGGCTATGTTTTACTT+TGG - chr7_3:58251046-58251065 Msa1112290:five_prime_UTR 35.0%
GAAAAGGGAAAAGGGAAAAG+TGG + chr7_3:58247854-58247873 None:intergenic 40.0%
GGGAAAGTGAAAAGGGAAAA+GGG + chr7_3:58247862-58247881 None:intergenic 40.0%
AGGGAAAGTGAAAAGGGAAA+AGG + chr7_3:58247863-58247882 None:intergenic 40.0%
GGGAAAAGGGAAAGTGAAAA+GGG + chr7_3:58247869-58247888 None:intergenic 40.0%
AGGGAAAAGGGAAAGTGAAA+AGG + chr7_3:58247870-58247889 None:intergenic 40.0%
ATTGTTCGATTGAGAACGGT+TGG + chr7_3:58247924-58247943 None:intergenic 40.0%
!! AGACTTGGTGAAGGTTGAAT+CGG + chr7_3:58247965-58247984 None:intergenic 40.0%
!! GTTGAAATCAGACTTGGTGA+AGG + chr7_3:58247974-58247993 None:intergenic 40.0%
TGAATCGAACCAAACAGCAA+AGG - chr7_3:58248098-58248117 Msa1112290:three_prime_UTR 40.0%
!! ATTCGGTTACCTTTGCTGTT+TGG + chr7_3:58248110-58248129 None:intergenic 40.0%
TTCTTCTTCCTTCGCGAATT+CGG + chr7_3:58248127-58248146 None:intergenic 40.0%
CAACAACAAACACAAGCTGT+CGG - chr7_3:58248181-58248200 Msa1112290:three_prime_UTR 40.0%
!! AAAGAATAGAGCCGTTTTCG+TGG - chr7_3:58248230-58248249 Msa1112290:three_prime_UTR 40.0%
!! TCCAACGAGATTTGCTATTC+CGG + chr7_3:58248437-58248456 None:intergenic 40.0%
GCTGCTTACAAACAACAGAA+GGG - chr7_3:58248527-58248546 Msa1112290:three_prime_UTR 40.0%
GAGAGATCAGACTTCTTAGT+TGG + chr7_3:58248607-58248626 None:intergenic 40.0%
TCAACAACTGTACTCAAACC+TGG - chr7_3:58248636-58248655 Msa1112290:three_prime_UTR 40.0%
CTGTACTCAAACCTGGAAAA+CGG - chr7_3:58248643-58248662 Msa1112290:three_prime_UTR 40.0%
ACAGTACATTGCGATGAACA+AGG - chr7_3:58248753-58248772 Msa1112290:intron 40.0%
AAGAACTCTTTCTGCTTCCA+GGG + chr7_3:58248799-58248818 None:intergenic 40.0%
AAAGAACTCTTTCTGCTTCC+AGG + chr7_3:58248800-58248819 None:intergenic 40.0%
AAAGAAGAAACATACCGCGA+GGG + chr7_3:58248872-58248891 None:intergenic 40.0%
CCAAAGACAAGTGTAAGAAC+AGG + chr7_3:58249210-58249229 None:intergenic 40.0%
CCTGTTCTTACACTTGTCTT+TGG - chr7_3:58249207-58249226 Msa1112290:intron 40.0%
ACTTCTGGCCAATTTGATGA+GGG - chr7_3:58249239-58249258 Msa1112290:intron 40.0%
GAAGAAAGCCCTCATCAAAT+TGG + chr7_3:58249250-58249269 None:intergenic 40.0%
! GCCTACCTATCTTATTTGCA+TGG - chr7_3:58249318-58249337 Msa1112290:intron 40.0%
TATTTGCATGGAGCATGGTA+TGG - chr7_3:58249330-58249349 Msa1112290:intron 40.0%
TTAAATATGGCACAGGAACC+AGG + chr7_3:58250085-58250104 None:intergenic 40.0%
TACTCCCATAGATTGTCTCA+AGG - chr7_3:58250593-58250612 Msa1112290:intron 40.0%
ACTCCCATAGATTGTCTCAA+GGG - chr7_3:58250594-58250613 Msa1112290:intron 40.0%
ATCCCCTTGAGACAATCTAT+GGG + chr7_3:58250600-58250619 None:intergenic 40.0%
TATCCCCTTGAGACAATCTA+TGG + chr7_3:58250601-58250620 None:intergenic 40.0%
AAGAAATTCCACCTCCATCT+CGG - chr7_3:58250682-58250701 Msa1112290:intron 40.0%
TCATTAAATTGCCGAGATGG+AGG + chr7_3:58250696-58250715 None:intergenic 40.0%
AATGAGAAGTGATAGGAGCT+AGG - chr7_3:58250736-58250755 Msa1112290:intron 40.0%
TCTGATGTATAAGCTCCTGA+TGG + chr7_3:58250767-58250786 None:intergenic 40.0%
GAAGGGGAGAAAAGGGAAAA+GGG + chr7_3:58247882-58247901 None:intergenic 45.0%
TGAAGGGGAGAAAAGGGAAA+AGG + chr7_3:58247883-58247902 None:intergenic 45.0%
TGGAGAGTTTCACGAGTGAA+GGG + chr7_3:58247899-58247918 None:intergenic 45.0%
TTGGAGAGTTTCACGAGTGA+AGG + chr7_3:58247900-58247919 None:intergenic 45.0%
! TCGATTGAGAACGGTTGGTT+TGG + chr7_3:58247919-58247938 None:intergenic 45.0%
! CAGCTTTGTGCTTCTGACTT+GGG + chr7_3:58248052-58248071 None:intergenic 45.0%
! TCAGCTTTGTGCTTCTGACT+TGG + chr7_3:58248053-58248072 None:intergenic 45.0%
! CAGAAGCACAAAGCTGAGTT+TGG - chr7_3:58248056-58248075 Msa1112290:three_prime_UTR 45.0%
AAAGGTAACCGAATTCGCGA+AGG - chr7_3:58248116-58248135 Msa1112290:three_prime_UTR 45.0%
CCTCTGTTCTTCCACGAAAA+CGG + chr7_3:58248244-58248263 None:intergenic 45.0%
AGCAGCGATGGTTTCATAAG+AGG + chr7_3:58248513-58248532 None:intergenic 45.0%
! CTGTTGTTTGTAAGCAGCGA+TGG + chr7_3:58248525-58248544 None:intergenic 45.0%
CGCTGCTTACAAACAACAGA+AGG - chr7_3:58248526-58248545 Msa1112290:three_prime_UTR 45.0%
CTTCTTAGTTGGGAGATCGA+AGG + chr7_3:58248596-58248615 None:intergenic 45.0%
TCTCTTGCGATTCCACGATT+GGG - chr7_3:58248681-58248700 Msa1112290:three_prime_UTR 45.0%
! ACTATAAGAAGAGTGCACCC+TGG - chr7_3:58248779-58248798 Msa1112290:intron 45.0%
GAAAGAAGAAACATACCGCG+AGG + chr7_3:58248873-58248892 None:intergenic 45.0%
ACAACTATTCGGTTTACGCC+CGG - chr7_3:58248992-58249011 Msa1112290:exon 45.0%
TGATTAGGTATGAGCCATCC+CGG + chr7_3:58249014-58249033 None:intergenic 45.0%
CACTTCTGGCCAATTTGATG+AGG - chr7_3:58249238-58249257 Msa1112290:intron 45.0%
GCATCTGAGGGGAAACTAAA+TGG - chr7_3:58249991-58250010 Msa1112290:intron 45.0%
CTGAGGGGAAACTAAATGGT+TGG - chr7_3:58249995-58250014 Msa1112290:intron 45.0%
GGCGAAAAGAACGAAGACAT+AGG - chr7_3:58250550-58250569 Msa1112290:intron 45.0%
CTCCCATAGATTGTCTCAAG+GGG - chr7_3:58250595-58250614 Msa1112290:intron 45.0%
TTAAATTGCCGAGATGGAGG+TGG + chr7_3:58250693-58250712 None:intergenic 45.0%
!!! GTGAGACTGGTTTTTGGAGT+AGG - chr7_3:58250897-58250916 Msa1112290:five_prime_UTR 45.0%
!!! AGACTGGTTTTTGGAGTAGG+TGG - chr7_3:58250900-58250919 Msa1112290:five_prime_UTR 45.0%
!!! GGTTTTTGGAGTAGGTGGTT+CGG - chr7_3:58250905-58250924 Msa1112290:five_prime_UTR 45.0%
GGGAAAAGGGAAAAGTGGGA+GGG + chr7_3:58247849-58247868 None:intergenic 50.0%
AGGGAAAAGGGAAAAGTGGG+AGG + chr7_3:58247850-58247869 None:intergenic 50.0%
CACGAGTGAAGGGGAGAAAA+GGG + chr7_3:58247889-58247908 None:intergenic 50.0%
TCACGAGTGAAGGGGAGAAA+AGG + chr7_3:58247890-58247909 None:intergenic 50.0%
GGAGAGTTTCACGAGTGAAG+GGG + chr7_3:58247898-58247917 None:intergenic 50.0%
! CCGTTTTCGTGGAAGAACAG+AGG - chr7_3:58248241-58248260 Msa1112290:three_prime_UTR 50.0%
! AAGAGTTTGAAACTCCGGCG+CGG - chr7_3:58248388-58248407 Msa1112290:three_prime_UTR 50.0%
GCCGGAATAGCAAATCTCGT+TGG - chr7_3:58248433-58248452 Msa1112290:three_prime_UTR 50.0%
TTGGGAGATCGAAGGAAGAC+AGG + chr7_3:58248588-58248607 None:intergenic 50.0%
TCAAACCTGGAAAACGGACG+CGG - chr7_3:58248649-58248668 Msa1112290:three_prime_UTR 50.0%
TCGTGGAATCGCAAGAGAGA+AGG + chr7_3:58248679-58248698 None:intergenic 50.0%
CTCTCTTGCGATTCCACGAT+TGG - chr7_3:58248680-58248699 Msa1112290:three_prime_UTR 50.0%
CTCTTGCGATTCCACGATTG+GGG - chr7_3:58248682-58248701 Msa1112290:three_prime_UTR 50.0%
CAACTATTCGGTTTACGCCC+GGG - chr7_3:58248993-58249012 Msa1112290:exon 50.0%
GATTAGGTATGAGCCATCCC+GGG + chr7_3:58249013-58249032 None:intergenic 50.0%
AGGATGTCTCAGATGCTCAG+TGG - chr7_3:58249163-58249182 Msa1112290:intron 50.0%
ATGTCTCAGATGCTCAGTGG+AGG - chr7_3:58249166-58249185 Msa1112290:intron 50.0%
! GCTCAGTGGAGGAATTTTCG+AGG - chr7_3:58249177-58249196 Msa1112290:intron 50.0%
TGAGAGGAATGTCCGTTGTC+TGG - chr7_3:58249280-58249299 Msa1112290:intron 50.0%
ACAGTATCCGACAGCATCTG+AGG - chr7_3:58249978-58249997 Msa1112290:intron 50.0%
CAGTATCCGACAGCATCTGA+GGG - chr7_3:58249979-58249998 Msa1112290:intron 50.0%
AGTATCCGACAGCATCTGAG+GGG - chr7_3:58249980-58249999 Msa1112290:intron 50.0%
AGTTTCCCCTCAGATGCTGT+CGG + chr7_3:58249988-58250007 None:intergenic 50.0%
AGGAGCTAGGCTAAACCATC+AGG - chr7_3:58250749-58250768 Msa1112290:intron 50.0%
AACATAGCCTATGCACTGCC+AGG + chr7_3:58251040-58251059 None:intergenic 50.0%
GCAGCTCGAATCCGAAGAAG+AGG - chr7_3:58248206-58248225 Msa1112290:three_prime_UTR 55.0%
!! GATTTGCTATTCCGGCGAGG+TGG + chr7_3:58248429-58248448 None:intergenic 55.0%
!! CGAGATTTGCTATTCCGGCG+AGG + chr7_3:58248432-58248451 None:intergenic 55.0%
TGTGAATAGCGCCCCAATCG+TGG + chr7_3:58248696-58248715 None:intergenic 55.0%
TATTCGGTTTACGCCCGGGA+TGG - chr7_3:58248997-58249016 Msa1112290:exon 55.0%
CTCTTAGCCTGGCAGTGCAT+AGG - chr7_3:58251030-58251049 Msa1112290:five_prime_UTR 55.0%
! AGTTTGAAACTCCGGCGCGG+TGG - chr7_3:58248391-58248410 Msa1112290:three_prime_UTR 60.0%
! GAACACCGCGTCCGTTTTCC+AGG + chr7_3:58248657-58248676 None:intergenic 60.0%
TGCTATTCCGGCGAGGTGGC+CGG + chr7_3:58248425-58248444 None:intergenic 65.0%
CATCATCAGGCGCTCCCTCG+CGG - chr7_3:58248855-58248874 Msa1112290:intron 65.0%
! CGGTGATGGCACCACCGCGC+CGG + chr7_3:58248405-58248424 None:intergenic 75.0%
!! CGGCGCGGTGGTGCCATCAC+CGG - chr7_3:58248403-58248422 Msa1112290:three_prime_UTR 75.0%
GCCATCACCGGCCACCTCGC+CGG - chr7_3:58248415-58248434 Msa1112290:three_prime_UTR 75.0%
TCCGGCGAGGTGGCCGGTGA+TGG + chr7_3:58248419-58248438 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 58247825 58251195 58247825 ID=Msa1112290;Name=Msa1112290
chr7_3 mRNA 58247825 58251195 58247825 ID=Msa1112290-mRNA-1;Parent=Msa1112290;Name=Msa1112290-mRNA-1;_AED=0.14;_eAED=0.14;_QI=659|1|1|1|0.6|0.5|6|966|127
chr7_3 exon 58247825 58248767 58247825 ID=Msa1112290-mRNA-1:exon:12748;Parent=Msa1112290-mRNA-1
chr7_3 exon 58248988 58249052 58248988 ID=Msa1112290-mRNA-1:exon:12747;Parent=Msa1112290-mRNA-1
chr7_3 exon 58249537 58249585 58249537 ID=Msa1112290-mRNA-1:exon:12746;Parent=Msa1112290-mRNA-1
chr7_3 exon 58249674 58249855 58249674 ID=Msa1112290-mRNA-1:exon:12745;Parent=Msa1112290-mRNA-1
chr7_3 exon 58250144 58250475 58250144 ID=Msa1112290-mRNA-1:exon:12744;Parent=Msa1112290-mRNA-1
chr7_3 exon 58250758 58251195 58250758 ID=Msa1112290-mRNA-1:exon:12743;Parent=Msa1112290-mRNA-1
chr7_3 five_prime_UTR 58250758 58251195 58250758 ID=Msa1112290-mRNA-1:five_prime_utr;Parent=Msa1112290-mRNA-1
chr7_3 five_prime_UTR 58250255 58250475 58250255 ID=Msa1112290-mRNA-1:five_prime_utr;Parent=Msa1112290-mRNA-1
chr7_3 CDS 58250144 58250254 58250144 ID=Msa1112290-mRNA-1:cds;Parent=Msa1112290-mRNA-1
chr7_3 CDS 58249674 58249855 58249674 ID=Msa1112290-mRNA-1:cds;Parent=Msa1112290-mRNA-1
chr7_3 CDS 58249537 58249585 58249537 ID=Msa1112290-mRNA-1:cds;Parent=Msa1112290-mRNA-1
chr7_3 CDS 58249011 58249052 58249011 ID=Msa1112290-mRNA-1:cds;Parent=Msa1112290-mRNA-1
chr7_3 three_prime_UTR 58248988 58249010 58248988 ID=Msa1112290-mRNA-1:three_prime_utr;Parent=Msa1112290-mRNA-1
chr7_3 three_prime_UTR 58247825 58248767 58247825 ID=Msa1112290-mRNA-1:three_prime_utr;Parent=Msa1112290-mRNA-1
Gene Sequence

>Msa1112290

ATGAACAAGGACAACTATAAGAAGAGTGCACCCTGGAAGCAGAAAGAGTTCTTTTTTCTCGTCACTTATGCATTCATTTTCTATGTTATCATCATCAGGCGCTCCCTCGCGGATGTCTCAGATGCTCAGTGGAGGAATTTTCGAGGAAATTTACCTGTTCTTACACTTGTCTTTGGAATCTTTACACTTCTGGCCAATTTGATGAGGGCTTTCTTCAATTTCAATGTGAGAGGAATGTCCGTTGTCTGGCTTTTGTTTTCTTTCGCCTACCTATCTTATTTGCATGGAGCATGCGTCATATTTGTGCTCTCAATAGCTACTATTAATTTTCTTCTTGTTAAGTCATTGTCGAACAGTATCCGACAGCATCTGAGGGGAAACTAA
Protein sequence

>Msa1112290

MNKDNYKKSAPWKQKEFFFLVTYAFIFYVIIIRRSLADVSDAQWRNFRGNLPVLTLVFGIFTLLANLMRAFFNFNVRGMSVVWLLFSFAYLSYLHGACVIFVLSIATINFLLVKSLSNSIRQHLRGN*