Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0353240 | XP_024633996.1 | 91.176 | 68 | 6 | 0 | 1 | 68 | 1 | 68 | 4.06e-34 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0353240 | G7IZF3 | 91.176 | 68 | 6 | 0 | 1 | 68 | 1 | 68 | 1.94e-34 | 121 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0353240 | Msa0447040 | 0.844721 | 6.343414e-59 | -8.615850e-47 |
| Msa0353240 | Msa0494560 | 0.818415 | 1.983654e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCGTTGGCTCCGGCACC+AGG | 0.362809 | 3_1:-23042178 | None:intergenic |
| GGTCCTGATGCTGGAGCTGC+TGG | 0.374025 | 3_1:-23042157 | None:intergenic |
| ATGATGCCATTGCAAAACTA+TGG | 0.400617 | 3_1:+23042275 | Msa0353240:CDS |
| CCGGCACCAGGTCCTGATGC+TGG | 0.402695 | 3_1:-23042166 | None:intergenic |
| CCAACGAGGAACTGAGGTCT+TGG | 0.404931 | 3_1:+23042191 | Msa0353240:CDS |
| GATCCAGCAGCTCCAGCATC+AGG | 0.425147 | 3_1:+23042154 | Msa0353240:CDS |
| GGCCATGGTGGCAGCGAAGA+TGG | 0.449950 | 3_1:+23042219 | Msa0353240:CDS |
| GAGGTCTTGGGCAGAGGCCA+TGG | 0.488985 | 3_1:+23042204 | Msa0353240:CDS |
| CCAGCATCAGGACCTGGTGC+CGG | 0.497543 | 3_1:+23042166 | Msa0353240:CDS |
| CAACGAGGAACTGAGGTCTT+GGG | 0.499775 | 3_1:+23042192 | Msa0353240:CDS |
| GGAACTGAGGTCTTGGGCAG+AGG | 0.510456 | 3_1:+23042198 | Msa0353240:CDS |
| GCAGCTCCAGCATCAGGACC+TGG | 0.528586 | 3_1:+23042160 | Msa0353240:CDS |
| CCTCAGTTCCTCGTTGGCTC+CGG | 0.539242 | 3_1:-23042185 | None:intergenic |
| CGCCATCTTCGCTGCCACCA+TGG | 0.550043 | 3_1:-23042221 | None:intergenic |
| CCAAGACCTCAGTTCCTCGT+TGG | 0.560587 | 3_1:-23042191 | None:intergenic |
| GTCTTGGGCAGAGGCCATGG+TGG | 0.571711 | 3_1:+23042207 | Msa0353240:CDS |
| ACAAGTTCAGTGGCTATGAT+CGG | 0.577020 | 3_1:-23042127 | None:intergenic |
| CCGGAGCCAACGAGGAACTG+AGG | 0.644289 | 3_1:+23042185 | Msa0353240:CDS |
| TGGATCTGTTACAAGTTCAG+TGG | 0.658686 | 3_1:-23042137 | None:intergenic |
| ACCTGGTGCCGGAGCCAACG+AGG | 0.667046 | 3_1:+23042177 | Msa0353240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTATTCCATAGTTTTGCAA+TGG | - | chr3_1:23042284-23042303 | None:intergenic | 30.0% |
| ATGATGCCATTGCAAAACTA+TGG | + | chr3_1:23042275-23042294 | Msa0353240:CDS | 35.0% | |
| ACAAGTTCAGTGGCTATGAT+CGG | - | chr3_1:23042130-23042149 | None:intergenic | 40.0% | |
| TGGATCTGTTACAAGTTCAG+TGG | - | chr3_1:23042140-23042159 | None:intergenic | 40.0% | |
| GAAGATGGCGACGAAAAAGA+AGG | + | chr3_1:23042234-23042253 | Msa0353240:CDS | 45.0% | |
| CAACGAGGAACTGAGGTCTT+GGG | + | chr3_1:23042192-23042211 | Msa0353240:CDS | 50.0% | |
| CCAAGACCTCAGTTCCTCGT+TGG | - | chr3_1:23042194-23042213 | None:intergenic | 55.0% | |
| CCAACGAGGAACTGAGGTCT+TGG | + | chr3_1:23042191-23042210 | Msa0353240:CDS | 55.0% | |
| GATCCAGCAGCTCCAGCATC+AGG | + | chr3_1:23042154-23042173 | Msa0353240:CDS | 60.0% | |
| !! | CCTCAGTTCCTCGTTGGCTC+CGG | - | chr3_1:23042188-23042207 | None:intergenic | 60.0% |
| GGAACTGAGGTCTTGGGCAG+AGG | + | chr3_1:23042198-23042217 | Msa0353240:CDS | 60.0% | |
| GGTCCTGATGCTGGAGCTGC+TGG | - | chr3_1:23042160-23042179 | None:intergenic | 65.0% | |
| GCAGCTCCAGCATCAGGACC+TGG | + | chr3_1:23042160-23042179 | Msa0353240:CDS | 65.0% | |
| CCAGCATCAGGACCTGGTGC+CGG | + | chr3_1:23042166-23042185 | Msa0353240:CDS | 65.0% | |
| CCGGAGCCAACGAGGAACTG+AGG | + | chr3_1:23042185-23042204 | Msa0353240:CDS | 65.0% | |
| GAGGTCTTGGGCAGAGGCCA+TGG | + | chr3_1:23042204-23042223 | Msa0353240:CDS | 65.0% | |
| GTCTTGGGCAGAGGCCATGG+TGG | + | chr3_1:23042207-23042226 | Msa0353240:CDS | 65.0% | |
| GGCCATGGTGGCAGCGAAGA+TGG | + | chr3_1:23042219-23042238 | Msa0353240:CDS | 65.0% | |
| CGCCATCTTCGCTGCCACCA+TGG | - | chr3_1:23042224-23042243 | None:intergenic | 65.0% | |
| CCGGCACCAGGTCCTGATGC+TGG | - | chr3_1:23042169-23042188 | None:intergenic | 70.0% | |
| ! | ACCTGGTGCCGGAGCCAACG+AGG | + | chr3_1:23042177-23042196 | Msa0353240:CDS | 70.0% |
| !! | TCCTCGTTGGCTCCGGCACC+AGG | - | chr3_1:23042181-23042200 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 23042097 | 23042309 | 23042097 | ID=Msa0353240;Name=Msa0353240 |
| chr3_1 | mRNA | 23042097 | 23042309 | 23042097 | ID=Msa0353240-mRNA-1;Parent=Msa0353240;Name=Msa0353240-mRNA-1;_AED=0.35;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|70 |
| chr3_1 | exon | 23042097 | 23042309 | 23042097 | ID=Msa0353240-mRNA-1:exon:5183;Parent=Msa0353240-mRNA-1 |
| chr3_1 | CDS | 23042097 | 23042309 | 23042097 | ID=Msa0353240-mRNA-1:cds;Parent=Msa0353240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
