AlfalfaGEDB | Msa0447040

M. sativa cultivar zhongmu-4 / Msa0447040

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0447040	PNX54969.1	81.250	64	12	0	2	65	5	68	8.94e-27	102

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0447040	A0A2K3JLR2	81.250	64	12	0	2	65	5	68	4.27e-27	102

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0036610	Msa0447040	0.801900	7.238512e-49	-8.615850e-47
Msa0353240	Msa0447040	0.844721	6.343414e-59	-8.615850e-47
Msa0353250	Msa0447040	0.832252	1.051294e-55	-8.615850e-47
Msa0447040	Msa0494560	0.908666	1.331940e-81	-8.615850e-47
Msa0447040	Msa0509020	-0.823440	1.383394e-53	-8.615850e-47
Msa0447040	Msa0691080	-0.800055	1.724570e-48	-8.615850e-47
Msa0447040	Msa0886660	-0.805851	1.093541e-49	-8.615850e-47
Msa0447040	Msa0968320	0.813259	2.801950e-51	-8.615850e-47
Msa0447040	Msa1249370	0.809671	1.686293e-50	-8.615850e-47
Msa0447040	Msa1371080	0.806589	7.643989e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0447040	MtrunA17_Chr3g0088011	81.250	64	12	0	2	65	3	66	9.71e-31	102
Msa0447040	MtrunA17_Chr3g0088251	81.250	64	12	0	2	65	3	66	1.59e-30	101
Msa0447040	MtrunA17_Chr3g0088031	81.967	61	11	0	5	65	1	61	3.51e-30	100
Msa0447040	MtrunA17_Chr3g0087981	76.562	64	15	0	2	65	5	68	5.56e-30	100
Msa0447040	MtrunA17_Chr3g0088021	79.688	64	13	0	2	65	3	66	1.38e-29	99.4
Msa0447040	MtrunA17_Chr3g0087921	82.812	64	9	2	2	65	6	67	4.38e-27	92.8
Msa0447040	MtrunA17_Chr3g0087931	80.952	63	10	2	3	65	7	67	5.70e-27	92.8
Msa0447040	MtrunA17_Chr3g0088071	81.250	64	12	0	2	65	3	66	5.91e-24	85.1
Msa0447040	MtrunA17_Chr3g0088081	93.846	65	4	0	1	65	1	65	7.17e-24	84.7
Msa0447040	MtrunA17_Chr3g0088271	83.077	65	11	0	1	65	1	65	6.37e-23	82.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 18 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAATTCAGTTGCTATGAT+TGG	0.314744	3_3:+24332808	Msa0447040:CDS
ACCTGGTGCCGGAGCCAACG+AGG	0.656634	3_3:-24332758	None:intergenic
AGATCCAGCAGCACCAGCAT+CGG	0.601129	3_3:-24332782	None:intergenic
AGTTGCTATGATTGGAGCTT+CGG	0.447713	3_3:+24332816	Msa0447040:CDS
CCAGCATCGGGACCTGGTGC+CGG	0.481084	3_3:-24332769	None:intergenic
CCCTCGTTGGCTCCGGCACC+AGG	0.346432	3_3:+24332757	Msa0447040:CDS
CCGGAGCCAACGAGGGACTG+AGG	0.619509	3_3:-24332750	None:intergenic
CCGGCACCAGGTCCCGATGC+TGG	0.434291	3_3:+24332769	Msa0447040:CDS
CCTCAGTCCCTCGTTGGCTC+CGG	0.529105	3_3:+24332750	Msa0447040:CDS
CCTGGTGCCGGAGCCAACGA+GGG	0.645258	3_3:-24332757	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAATTCAGTTGCTATGAT+TGG	+	chr3_3:24332808-24332827	Msa0447040:CDS	30.0%
AGTTGCTATGATTGGAGCTT+CGG	+	chr3_3:24332816-24332835	Msa0447040:CDS	40.0%
TCAAGACCTCAGTCCCTCGT+TGG	+	chr3_3:24332744-24332763	Msa0447040:CDS	55.0%
AGATCCAGCAGCACCAGCAT+CGG	-	chr3_3:24332785-24332804	None:intergenic	55.0%
GAGGTCTTGAGCCGAAGCCA+CGG	-	chr3_3:24332734-24332753	None:intergenic	60.0%
GATCCAGCAGCACCAGCATC+GGG	-	chr3_3:24332784-24332803	None:intergenic	60.0%
CGTTGTCTTCGCAGCAGCCG+TGG	+	chr3_3:24332714-24332733	Msa0447040:CDS	65.0%
CTTCGCAGCAGCCGTGGCTT+CGG	+	chr3_3:24332720-24332739	Msa0447040:CDS	65.0%
CCGGAGCCAACGAGGGACTG+AGG	-	chr3_3:24332753-24332772	None:intergenic	70.0%
CCGGCACCAGGTCCCGATGC+TGG	+	chr3_3:24332769-24332788	Msa0447040:CDS	75.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	24332676	24332873	24332676	ID=Msa0447040;Name=Msa0447040
chr3_3	mRNA	24332676	24332873	24332676	ID=Msa0447040-mRNA-1;Parent=Msa0447040;Name=Msa0447040-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|65
chr3_3	exon	24332676	24332873	24332676	ID=Msa0447040-mRNA-1:exon:4840;Parent=Msa0447040-mRNA-1
chr3_3	CDS	24332676	24332873	24332676	ID=Msa0447040-mRNA-1:cds;Parent=Msa0447040-mRNA-1

Gene Sequence	>Msa0447040 ATGGCATCATCAATGAAGTTTTTCGTCTTCTTTTTCGTCGTTGTCTTCGCAGCAGCCGTGGCTTCGGCTCAAGACCTCAGTCCCTCGTTGGCTCCGGCACCAGGTCCCGATGCTGGTGCTGCTGGATCTGTTACAAATTCAGTTGCTATGATTGGAGCTTCGGTAGTTTTGTCTATGCTCGCCATCTTCAAGAACTAA
Protein sequence	>Msa0447040 MASSMKFFVFFFVVVFAAAVASAQDLSPSLAPAPGPDAGAAGSVTNSVAMIGASVVLSMLAIFKN*