Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0355350 | Msa0450570 | 0.802251 | 6.130922e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATAGGTTTGTTGATGT+TGG | 0.328111 | 3_1:-29461008 | Msa0355350:CDS |
| AGAGGCTATTGATCAGTTCT+TGG | 0.371669 | 3_1:-29460382 | Msa0355350:CDS |
| AATGTTCTTTGTTGTTCAAA+AGG | 0.386291 | 3_1:-29460316 | Msa0355350:three_prime_UTR |
| GTGATATTAATGTTCAATTG+AGG | 0.478380 | 3_1:-29460977 | Msa0355350:CDS |
| TCTAACTGAGTCTAAGAGTT+GGG | 0.483948 | 3_1:-29460415 | Msa0355350:CDS |
| TGATATTAATGTTCAATTGA+GGG | 0.490450 | 3_1:-29460976 | Msa0355350:CDS |
| AATTGAACATTAATATCACT+TGG | 0.491523 | 3_1:+29460981 | None:intergenic |
| AGGTGCATACCTAGTGAAGA+TGG | 0.527872 | 3_1:+29460944 | None:intergenic |
| ATCTAACTGAGTCTAAGAGT+TGG | 0.533485 | 3_1:-29460416 | Msa0355350:CDS |
| TGTTACTTTCCATCTTCACT+AGG | 0.550768 | 3_1:-29460953 | Msa0355350:intron |
| ATTGAACATTAATATCACTT+GGG | 0.576242 | 3_1:+29460982 | None:intergenic |
| AGTCTAAGAGTTGGGATGTG+AGG | 0.603673 | 3_1:-29460407 | Msa0355350:CDS |
| TTCAAGAGAAACAATACATG+AGG | 0.638964 | 3_1:+29460337 | None:intergenic |
| GAGTTGGGATGTGAGGAAAG+AGG | 0.654443 | 3_1:-29460400 | Msa0355350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAATTATTATTAATAGGTT+TGG | + | chr3_1:29460763-29460782 | None:intergenic | 15.0% |
| !! | CACAACAAATTATTATTAAT+AGG | + | chr3_1:29460768-29460787 | None:intergenic | 15.0% |
| !! | CAAATTATTATTAATAGGTT+TGG | + | chr3_1:29460763-29460782 | None:intergenic | 15.0% |
| !! | CACAACAAATTATTATTAAT+AGG | + | chr3_1:29460768-29460787 | None:intergenic | 15.0% |
| !! | ATTGAACATTAATATCACTT+GGG | + | chr3_1:29460262-29460281 | None:intergenic | 20.0% |
| !! | AATTGAACATTAATATCACT+TGG | + | chr3_1:29460263-29460282 | None:intergenic | 20.0% |
| !! | TGATATTAATGTTCAATTGA+GGG | - | chr3_1:29460265-29460284 | Msa0355350:three_prime_UTR | 20.0% |
| !! | AAAGTACAAATATGAAATGA+AGG | + | chr3_1:29460320-29460339 | None:intergenic | 20.0% |
| !! | AAATATGTGCACTAAAAATA+TGG | - | chr3_1:29460428-29460447 | Msa0355350:CDS | 20.0% |
| !!! | TGCAATGATTTATGTAATTT+TGG | - | chr3_1:29460682-29460701 | Msa0355350:intron | 20.0% |
| !!! | GCAATGATTTATGTAATTTT+GGG | - | chr3_1:29460683-29460702 | Msa0355350:intron | 20.0% |
| !! | AAACTAATAACGATAATCAA+TGG | + | chr3_1:29460737-29460756 | None:intergenic | 20.0% |
| !! | ATTGAACATTAATATCACTT+GGG | + | chr3_1:29460262-29460281 | None:intergenic | 20.0% |
| !! | AATTGAACATTAATATCACT+TGG | + | chr3_1:29460263-29460282 | None:intergenic | 20.0% |
| !! | TGATATTAATGTTCAATTGA+GGG | - | chr3_1:29460265-29460284 | Msa0355350:three_prime_UTR | 20.0% |
| !! | AAAGTACAAATATGAAATGA+AGG | + | chr3_1:29460320-29460339 | None:intergenic | 20.0% |
| !! | AAATATGTGCACTAAAAATA+TGG | - | chr3_1:29460428-29460447 | Msa0355350:CDS | 20.0% |
| !!! | TGCAATGATTTATGTAATTT+TGG | - | chr3_1:29460682-29460701 | Msa0355350:intron | 20.0% |
| !!! | GCAATGATTTATGTAATTTT+GGG | - | chr3_1:29460683-29460702 | Msa0355350:intron | 20.0% |
| !! | AAACTAATAACGATAATCAA+TGG | + | chr3_1:29460737-29460756 | None:intergenic | 20.0% |
| ! | GTGATATTAATGTTCAATTG+AGG | - | chr3_1:29460264-29460283 | Msa0355350:three_prime_UTR | 25.0% |
| !! | TATGTTTAAGACGTGATATT+CGG | + | chr3_1:29460377-29460396 | None:intergenic | 25.0% |
| ! | TTAAATTGTAGCTAGAACAA+TGG | - | chr3_1:29460517-29460536 | Msa0355350:intron | 25.0% |
| !! | GTTATAAATTTTGCATGTTG+CGG | + | chr3_1:29460638-29460657 | None:intergenic | 25.0% |
| !!! | AATGTTCTTTGTTGTTCAAA+AGG | - | chr3_1:29460925-29460944 | Msa0355350:intron | 25.0% |
| ! | GTGATATTAATGTTCAATTG+AGG | - | chr3_1:29460264-29460283 | Msa0355350:three_prime_UTR | 25.0% |
| !! | TATGTTTAAGACGTGATATT+CGG | + | chr3_1:29460377-29460396 | None:intergenic | 25.0% |
| ! | TTAAATTGTAGCTAGAACAA+TGG | - | chr3_1:29460517-29460536 | Msa0355350:intron | 25.0% |
| !! | GTTATAAATTTTGCATGTTG+CGG | + | chr3_1:29460638-29460657 | None:intergenic | 25.0% |
| !!! | AATGTTCTTTGTTGTTCAAA+AGG | - | chr3_1:29460925-29460944 | Msa0355350:intron | 25.0% |
| !! | TTTCATAGGTTTGTTGATGT+TGG | - | chr3_1:29460233-29460252 | Msa0355350:three_prime_UTR | 30.0% |
| CTATCGATAGATTACTAGTA+GGG | + | chr3_1:29460345-29460364 | None:intergenic | 30.0% | |
| ACTATCGATAGATTACTAGT+AGG | + | chr3_1:29460346-29460365 | None:intergenic | 30.0% | |
| ! | CAAAAGTATAAACATGTCAG+AGG | - | chr3_1:29460397-29460416 | Msa0355350:CDS | 30.0% |
| TGCACTAAAAATATGGTGAA+GGG | - | chr3_1:29460435-29460454 | Msa0355350:CDS | 30.0% | |
| AACAAGAATCACACAACAAA+AGG | + | chr3_1:29460710-29460729 | None:intergenic | 30.0% | |
| TTCAAGAGAAACAATACATG+AGG | + | chr3_1:29460907-29460926 | None:intergenic | 30.0% | |
| !! | TTTCATAGGTTTGTTGATGT+TGG | - | chr3_1:29460233-29460252 | Msa0355350:three_prime_UTR | 30.0% |
| CTATCGATAGATTACTAGTA+GGG | + | chr3_1:29460345-29460364 | None:intergenic | 30.0% | |
| ACTATCGATAGATTACTAGT+AGG | + | chr3_1:29460346-29460365 | None:intergenic | 30.0% | |
| ! | CAAAAGTATAAACATGTCAG+AGG | - | chr3_1:29460397-29460416 | Msa0355350:CDS | 30.0% |
| TGCACTAAAAATATGGTGAA+GGG | - | chr3_1:29460435-29460454 | Msa0355350:CDS | 30.0% | |
| AACAAGAATCACACAACAAA+AGG | + | chr3_1:29460710-29460729 | None:intergenic | 30.0% | |
| TTCAAGAGAAACAATACATG+AGG | + | chr3_1:29460907-29460926 | None:intergenic | 30.0% | |
| !! | TGTTACTTTCCATCTTCACT+AGG | - | chr3_1:29460288-29460307 | Msa0355350:three_prime_UTR | 35.0% |
| GTGCACTAAAAATATGGTGA+AGG | - | chr3_1:29460434-29460453 | Msa0355350:CDS | 35.0% | |
| GCACTAAAAATATGGTGAAG+GGG | - | chr3_1:29460436-29460455 | Msa0355350:CDS | 35.0% | |
| AATTGTAGCTAGAACAATGG+AGG | - | chr3_1:29460520-29460539 | Msa0355350:intron | 35.0% | |
| ! | ATAAATTTTGCATGTTGCGG+CGG | + | chr3_1:29460635-29460654 | None:intergenic | 35.0% |
| ATCTAACTGAGTCTAAGAGT+TGG | - | chr3_1:29460825-29460844 | Msa0355350:intron | 35.0% | |
| TCTAACTGAGTCTAAGAGTT+GGG | - | chr3_1:29460826-29460845 | Msa0355350:intron | 35.0% | |
| !! | TGTTACTTTCCATCTTCACT+AGG | - | chr3_1:29460288-29460307 | Msa0355350:three_prime_UTR | 35.0% |
| GTGCACTAAAAATATGGTGA+AGG | - | chr3_1:29460434-29460453 | Msa0355350:CDS | 35.0% | |
| GCACTAAAAATATGGTGAAG+GGG | - | chr3_1:29460436-29460455 | Msa0355350:CDS | 35.0% | |
| AATTGTAGCTAGAACAATGG+AGG | - | chr3_1:29460520-29460539 | Msa0355350:intron | 35.0% | |
| ! | ATAAATTTTGCATGTTGCGG+CGG | + | chr3_1:29460635-29460654 | None:intergenic | 35.0% |
| ATCTAACTGAGTCTAAGAGT+TGG | - | chr3_1:29460825-29460844 | Msa0355350:intron | 35.0% | |
| TCTAACTGAGTCTAAGAGTT+GGG | - | chr3_1:29460826-29460845 | Msa0355350:intron | 35.0% | |
| GTAAAGTGGCAGTTTACTGT+TGG | + | chr3_1:29460546-29460565 | None:intergenic | 40.0% | |
| !! | AACTGCCACTTTACACATAG+CGG | - | chr3_1:29460552-29460571 | Msa0355350:intron | 40.0% |
| CATAGCGGCAGCAAAAATTA+CGG | - | chr3_1:29460567-29460586 | Msa0355350:intron | 40.0% | |
| ! | TTGTGTGTTGCTAAACTTGC+AGG | - | chr3_1:29460783-29460802 | Msa0355350:intron | 40.0% |
| AGAGGCTATTGATCAGTTCT+TGG | - | chr3_1:29460859-29460878 | Msa0355350:intron | 40.0% | |
| GTAAAGTGGCAGTTTACTGT+TGG | + | chr3_1:29460546-29460565 | None:intergenic | 40.0% | |
| !! | AACTGCCACTTTACACATAG+CGG | - | chr3_1:29460552-29460571 | Msa0355350:intron | 40.0% |
| CATAGCGGCAGCAAAAATTA+CGG | - | chr3_1:29460567-29460586 | Msa0355350:intron | 40.0% | |
| ! | TTGTGTGTTGCTAAACTTGC+AGG | - | chr3_1:29460783-29460802 | Msa0355350:intron | 40.0% |
| AGAGGCTATTGATCAGTTCT+TGG | - | chr3_1:29460859-29460878 | Msa0355350:intron | 40.0% | |
| AGGTGCATACCTAGTGAAGA+TGG | + | chr3_1:29460300-29460319 | None:intergenic | 45.0% | |
| ACCGCCAGTTTGGTATATAG+TGG | - | chr3_1:29460600-29460619 | Msa0355350:intron | 45.0% | |
| GCCACTATATACCAAACTGG+CGG | + | chr3_1:29460604-29460623 | None:intergenic | 45.0% | |
| GCCAGTTTGGTATATAGTGG+CGG | - | chr3_1:29460603-29460622 | Msa0355350:intron | 45.0% | |
| ! | AAGTCAAGTCGCAAGCATTG+CGG | + | chr3_1:29460660-29460679 | None:intergenic | 45.0% |
| AGTCTAAGAGTTGGGATGTG+AGG | - | chr3_1:29460834-29460853 | Msa0355350:intron | 45.0% | |
| AGGTGCATACCTAGTGAAGA+TGG | + | chr3_1:29460300-29460319 | None:intergenic | 45.0% | |
| ACCGCCAGTTTGGTATATAG+TGG | - | chr3_1:29460600-29460619 | Msa0355350:intron | 45.0% | |
| GCCACTATATACCAAACTGG+CGG | + | chr3_1:29460604-29460623 | None:intergenic | 45.0% | |
| GCCAGTTTGGTATATAGTGG+CGG | - | chr3_1:29460603-29460622 | Msa0355350:intron | 45.0% | |
| ! | AAGTCAAGTCGCAAGCATTG+CGG | + | chr3_1:29460660-29460679 | None:intergenic | 45.0% |
| AGTCTAAGAGTTGGGATGTG+AGG | - | chr3_1:29460834-29460853 | Msa0355350:intron | 45.0% | |
| GTGAAGGGGATGAAGCTGTT+TGG | - | chr3_1:29460450-29460469 | Msa0355350:intron | 50.0% | |
| TGCTGCCGCTATGTGTAAAG+TGG | + | chr3_1:29460560-29460579 | None:intergenic | 50.0% | |
| !!! | CAGTTTTGAGACCGCCAGTT+TGG | - | chr3_1:29460590-29460609 | Msa0355350:intron | 50.0% |
| GCCGCCACTATATACCAAAC+TGG | + | chr3_1:29460607-29460626 | None:intergenic | 50.0% | |
| GAGTTGGGATGTGAGGAAAG+AGG | - | chr3_1:29460841-29460860 | Msa0355350:intron | 50.0% | |
| GTGAAGGGGATGAAGCTGTT+TGG | - | chr3_1:29460450-29460469 | Msa0355350:intron | 50.0% | |
| TGCTGCCGCTATGTGTAAAG+TGG | + | chr3_1:29460560-29460579 | None:intergenic | 50.0% | |
| !!! | CAGTTTTGAGACCGCCAGTT+TGG | - | chr3_1:29460590-29460609 | Msa0355350:intron | 50.0% |
| GCCGCCACTATATACCAAAC+TGG | + | chr3_1:29460607-29460626 | None:intergenic | 50.0% | |
| GAGTTGGGATGTGAGGAAAG+AGG | - | chr3_1:29460841-29460860 | Msa0355350:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 29460224 | 29461039 | 29460224 | ID=Msa0355350;Name=Msa0355350 |
| chr3_1 | mRNA | 29460224 | 29461039 | 29460224 | ID=Msa0355350-mRNA-1;Parent=Msa0355350;Name=Msa0355350-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|1|0.5|1|1|1|2|114|68 |
| chr3_1 | exon | 29460224 | 29460458 | 29460224 | ID=Msa0355350-mRNA-1:exon:6141;Parent=Msa0355350-mRNA-1 |
| chr3_1 | exon | 29460954 | 29461039 | 29460954 | ID=Msa0355350-mRNA-1:exon:6140;Parent=Msa0355350-mRNA-1 |
| chr3_1 | CDS | 29460954 | 29461039 | 29460954 | ID=Msa0355350-mRNA-1:cds;Parent=Msa0355350-mRNA-1 |
| chr3_1 | CDS | 29460338 | 29460458 | 29460338 | ID=Msa0355350-mRNA-1:cds;Parent=Msa0355350-mRNA-1 |
| chr3_1 | three_prime_UTR | 29460224 | 29460337 | 29460224 | ID=Msa0355350-mRNA-1:three_prime_utr;Parent=Msa0355350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
