AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0450570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATAGGTTTGTTGATGT+TGG 0.328111 3_3:-36166307 Msa0450570:CDS
AGAGGCTATTGATCAGTTCT+TGG 0.371669 3_3:-36165681 Msa0450570:CDS
AATGTTCTTTGTTGTTCAAA+AGG 0.386291 3_3:-36165615 Msa0450570:three_prime_UTR
GTGATATTAATGTTCAATTG+AGG 0.478380 3_3:-36166276 Msa0450570:CDS
TGATATTAATGTTCAATTGA+GGG 0.490450 3_3:-36166275 Msa0450570:CDS
AATTGAACATTAATATCACT+TGG 0.491523 3_3:+36166280 None:intergenic
TCTGACTGAGTCTAAGAGTT+GGG 0.492505 3_3:-36165714 Msa0450570:CDS
AGGTGCATACCTAGTGAAGA+TGG 0.527872 3_3:+36166243 None:intergenic
ATCTGACTGAGTCTAAGAGT+TGG 0.540629 3_3:-36165715 Msa0450570:CDS
TGTTACTTTCCATCTTCACT+AGG 0.550768 3_3:-36166252 Msa0450570:intron
ATTGAACATTAATATCACTT+GGG 0.576242 3_3:+36166281 None:intergenic
AGTCTAAGAGTTGGGATGTG+AGG 0.603673 3_3:-36165706 Msa0450570:CDS
TTCAAGAGAAACAATACATG+AGG 0.638964 3_3:+36165636 None:intergenic
GAGTTGGGATGTGAGGAAAG+AGG 0.654443 3_3:-36165699 Msa0450570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATTATTATTAATAGGTT+TGG + chr3_3:36166062-36166081 None:intergenic 15.0%
!! CACAACAAATTATTATTAAT+AGG + chr3_3:36166067-36166086 None:intergenic 15.0%
!! CAAATTATTATTAATAGGTT+TGG + chr3_3:36166062-36166081 None:intergenic 15.0%
!! CACAACAAATTATTATTAAT+AGG + chr3_3:36166067-36166086 None:intergenic 15.0%
!! ATTGAACATTAATATCACTT+GGG + chr3_3:36165561-36165580 None:intergenic 20.0%
!! AATTGAACATTAATATCACT+TGG + chr3_3:36165562-36165581 None:intergenic 20.0%
!! TGATATTAATGTTCAATTGA+GGG - chr3_3:36165564-36165583 Msa0450570:three_prime_UTR 20.0%
!! AAAGTACAAATATGAAATGA+AGG + chr3_3:36165619-36165638 None:intergenic 20.0%
!! AAATATGTGCACTAAAAATA+TGG - chr3_3:36165727-36165746 Msa0450570:CDS 20.0%
!!! TGCAATGATTTATGTAATTT+TGG - chr3_3:36165981-36166000 Msa0450570:intron 20.0%
!!! GCAATGATTTATGTAATTTT+GGG - chr3_3:36165982-36166001 Msa0450570:intron 20.0%
!! AAACTAATAACGATAATCAA+TGG + chr3_3:36166036-36166055 None:intergenic 20.0%
!! ATTGAACATTAATATCACTT+GGG + chr3_3:36165561-36165580 None:intergenic 20.0%
!! AATTGAACATTAATATCACT+TGG + chr3_3:36165562-36165581 None:intergenic 20.0%
!! TGATATTAATGTTCAATTGA+GGG - chr3_3:36165564-36165583 Msa0450570:three_prime_UTR 20.0%
!! AAAGTACAAATATGAAATGA+AGG + chr3_3:36165619-36165638 None:intergenic 20.0%
!! AAATATGTGCACTAAAAATA+TGG - chr3_3:36165727-36165746 Msa0450570:CDS 20.0%
!!! TGCAATGATTTATGTAATTT+TGG - chr3_3:36165981-36166000 Msa0450570:intron 20.0%
!!! GCAATGATTTATGTAATTTT+GGG - chr3_3:36165982-36166001 Msa0450570:intron 20.0%
!! AAACTAATAACGATAATCAA+TGG + chr3_3:36166036-36166055 None:intergenic 20.0%
! GTGATATTAATGTTCAATTG+AGG - chr3_3:36165563-36165582 Msa0450570:three_prime_UTR 25.0%
!! TATGTTTAAGACGTGATATT+CGG + chr3_3:36165676-36165695 None:intergenic 25.0%
! TTAAATTGTAGCTAGAACAA+TGG - chr3_3:36165816-36165835 Msa0450570:intron 25.0%
!!! AATGTTCTTTGTTGTTCAAA+AGG - chr3_3:36166224-36166243 Msa0450570:intron 25.0%
! GTGATATTAATGTTCAATTG+AGG - chr3_3:36165563-36165582 Msa0450570:three_prime_UTR 25.0%
!! TATGTTTAAGACGTGATATT+CGG + chr3_3:36165676-36165695 None:intergenic 25.0%
! TTAAATTGTAGCTAGAACAA+TGG - chr3_3:36165816-36165835 Msa0450570:intron 25.0%
!!! AATGTTCTTTGTTGTTCAAA+AGG - chr3_3:36166224-36166243 Msa0450570:intron 25.0%
!! TTTCATAGGTTTGTTGATGT+TGG - chr3_3:36165532-36165551 Msa0450570:three_prime_UTR 30.0%
CTATCGATAGATTACTAGTA+GGG + chr3_3:36165644-36165663 None:intergenic 30.0%
ACTATCGATAGATTACTAGT+AGG + chr3_3:36165645-36165664 None:intergenic 30.0%
! CAAAAGTATAAACATGTCAG+AGG - chr3_3:36165696-36165715 Msa0450570:CDS 30.0%
TGCACTAAAAATATGGTGAA+GGG - chr3_3:36165734-36165753 Msa0450570:CDS 30.0%
! GGTATAAATTTTGCATGTTG+CGG + chr3_3:36165937-36165956 None:intergenic 30.0%
AACAAGAATCACACAACAAA+AGG + chr3_3:36166009-36166028 None:intergenic 30.0%
TTCAAGAGAAACAATACATG+AGG + chr3_3:36166206-36166225 None:intergenic 30.0%
!! TTTCATAGGTTTGTTGATGT+TGG - chr3_3:36165532-36165551 Msa0450570:three_prime_UTR 30.0%
CTATCGATAGATTACTAGTA+GGG + chr3_3:36165644-36165663 None:intergenic 30.0%
ACTATCGATAGATTACTAGT+AGG + chr3_3:36165645-36165664 None:intergenic 30.0%
! CAAAAGTATAAACATGTCAG+AGG - chr3_3:36165696-36165715 Msa0450570:CDS 30.0%
TGCACTAAAAATATGGTGAA+GGG - chr3_3:36165734-36165753 Msa0450570:CDS 30.0%
! GGTATAAATTTTGCATGTTG+CGG + chr3_3:36165937-36165956 None:intergenic 30.0%
AACAAGAATCACACAACAAA+AGG + chr3_3:36166009-36166028 None:intergenic 30.0%
TTCAAGAGAAACAATACATG+AGG + chr3_3:36166206-36166225 None:intergenic 30.0%
!! TGTTACTTTCCATCTTCACT+AGG - chr3_3:36165587-36165606 Msa0450570:three_prime_UTR 35.0%
GTGCACTAAAAATATGGTGA+AGG - chr3_3:36165733-36165752 Msa0450570:CDS 35.0%
GCACTAAAAATATGGTGAAG+GGG - chr3_3:36165735-36165754 Msa0450570:CDS 35.0%
AATTGTAGCTAGAACAATGG+AGG - chr3_3:36165819-36165838 Msa0450570:intron 35.0%
! ATAAATTTTGCATGTTGCGG+CGG + chr3_3:36165934-36165953 None:intergenic 35.0%
!! TGTTACTTTCCATCTTCACT+AGG - chr3_3:36165587-36165606 Msa0450570:three_prime_UTR 35.0%
GTGCACTAAAAATATGGTGA+AGG - chr3_3:36165733-36165752 Msa0450570:CDS 35.0%
GCACTAAAAATATGGTGAAG+GGG - chr3_3:36165735-36165754 Msa0450570:CDS 35.0%
AATTGTAGCTAGAACAATGG+AGG - chr3_3:36165819-36165838 Msa0450570:intron 35.0%
! ATAAATTTTGCATGTTGCGG+CGG + chr3_3:36165934-36165953 None:intergenic 35.0%
GTAAAGTGGCAGTTTACTGT+TGG + chr3_3:36165845-36165864 None:intergenic 40.0%
!! AACTGCCACTTTACACATAG+CGG - chr3_3:36165851-36165870 Msa0450570:intron 40.0%
CATAGCGGCAGCAAAAATTA+CGG - chr3_3:36165866-36165885 Msa0450570:intron 40.0%
! TTGTGTGTTGCTAAACTTGC+AGG - chr3_3:36166082-36166101 Msa0450570:intron 40.0%
ATCTGACTGAGTCTAAGAGT+TGG - chr3_3:36166124-36166143 Msa0450570:intron 40.0%
TCTGACTGAGTCTAAGAGTT+GGG - chr3_3:36166125-36166144 Msa0450570:intron 40.0%
AGAGGCTATTGATCAGTTCT+TGG - chr3_3:36166158-36166177 Msa0450570:intron 40.0%
GTAAAGTGGCAGTTTACTGT+TGG + chr3_3:36165845-36165864 None:intergenic 40.0%
!! AACTGCCACTTTACACATAG+CGG - chr3_3:36165851-36165870 Msa0450570:intron 40.0%
CATAGCGGCAGCAAAAATTA+CGG - chr3_3:36165866-36165885 Msa0450570:intron 40.0%
! TTGTGTGTTGCTAAACTTGC+AGG - chr3_3:36166082-36166101 Msa0450570:intron 40.0%
ATCTGACTGAGTCTAAGAGT+TGG - chr3_3:36166124-36166143 Msa0450570:intron 40.0%
TCTGACTGAGTCTAAGAGTT+GGG - chr3_3:36166125-36166144 Msa0450570:intron 40.0%
AGAGGCTATTGATCAGTTCT+TGG - chr3_3:36166158-36166177 Msa0450570:intron 40.0%
AGGTGCATACCTAGTGAAGA+TGG + chr3_3:36165599-36165618 None:intergenic 45.0%
ACCGCCAGTTTGGTATATAG+TGG - chr3_3:36165899-36165918 Msa0450570:intron 45.0%
GCCACTATATACCAAACTGG+CGG + chr3_3:36165903-36165922 None:intergenic 45.0%
GCCAGTTTGGTATATAGTGG+CGG - chr3_3:36165902-36165921 Msa0450570:intron 45.0%
! AAGTCAAGTCGCAAGCATTG+CGG + chr3_3:36165959-36165978 None:intergenic 45.0%
AGTCTAAGAGTTGGGATGTG+AGG - chr3_3:36166133-36166152 Msa0450570:intron 45.0%
AGGTGCATACCTAGTGAAGA+TGG + chr3_3:36165599-36165618 None:intergenic 45.0%
ACCGCCAGTTTGGTATATAG+TGG - chr3_3:36165899-36165918 Msa0450570:intron 45.0%
GCCACTATATACCAAACTGG+CGG + chr3_3:36165903-36165922 None:intergenic 45.0%
GCCAGTTTGGTATATAGTGG+CGG - chr3_3:36165902-36165921 Msa0450570:intron 45.0%
! AAGTCAAGTCGCAAGCATTG+CGG + chr3_3:36165959-36165978 None:intergenic 45.0%
AGTCTAAGAGTTGGGATGTG+AGG - chr3_3:36166133-36166152 Msa0450570:intron 45.0%
GTGAAGGGGATGAAGCTGTT+TGG - chr3_3:36165749-36165768 Msa0450570:intron 50.0%
TGCTGCCGCTATGTGTAAAG+TGG + chr3_3:36165859-36165878 None:intergenic 50.0%
!!! CAGTTTTGAGACCGCCAGTT+TGG - chr3_3:36165889-36165908 Msa0450570:intron 50.0%
GCCGCCACTATATACCAAAC+TGG + chr3_3:36165906-36165925 None:intergenic 50.0%
! AGTCAAGTCGCAAGCATTGC+GGG + chr3_3:36165958-36165977 None:intergenic 50.0%
GAGTTGGGATGTGAGGAAAG+AGG - chr3_3:36166140-36166159 Msa0450570:intron 50.0%
GTGAAGGGGATGAAGCTGTT+TGG - chr3_3:36165749-36165768 Msa0450570:intron 50.0%
TGCTGCCGCTATGTGTAAAG+TGG + chr3_3:36165859-36165878 None:intergenic 50.0%
!!! CAGTTTTGAGACCGCCAGTT+TGG - chr3_3:36165889-36165908 Msa0450570:intron 50.0%
GCCGCCACTATATACCAAAC+TGG + chr3_3:36165906-36165925 None:intergenic 50.0%
! AGTCAAGTCGCAAGCATTGC+GGG + chr3_3:36165958-36165977 None:intergenic 50.0%
GAGTTGGGATGTGAGGAAAG+AGG - chr3_3:36166140-36166159 Msa0450570:intron 50.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 36165523 36166338 36165523 ID=Msa0450570;Name=Msa0450570
chr3_3 mRNA 36165523 36166338 36165523 ID=Msa0450570-mRNA-1;Parent=Msa0450570;Name=Msa0450570-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|1|0.5|1|1|1|2|114|68
chr3_3 exon 36165523 36165757 36165523 ID=Msa0450570-mRNA-1:exon:6626;Parent=Msa0450570-mRNA-1
chr3_3 exon 36166253 36166338 36166253 ID=Msa0450570-mRNA-1:exon:6625;Parent=Msa0450570-mRNA-1
chr3_3 CDS 36166253 36166338 36166253 ID=Msa0450570-mRNA-1:cds;Parent=Msa0450570-mRNA-1
chr3_3 CDS 36165637 36165757 36165637 ID=Msa0450570-mRNA-1:cds;Parent=Msa0450570-mRNA-1
chr3_3 three_prime_UTR 36165523 36165636 36165523 ID=Msa0450570-mRNA-1:three_prime_utr;Parent=Msa0450570-mRNA-1
Gene Sequence

>Msa0450570

ATGTTTTATTTTCATAGGTTTGTTGATGTTGGATTCCCAAGTGATATTAATGTTCAATTGAGGGTGTTACTTTCCATCTTCACTAGGATATGTGACTATTACTCTTATCTGACTGAGTCTAAGAGTTGGGATGTGAGGAAAGAGGCTATTGATCAGTTCTTGGAAGCATATCAGTTATGCACACACCTCATGTATTGTTTCTCTTGA

Protein sequence

>Msa0450570

MFYFHRFVDVGFPSDINVQLRVLLSIFTRICDYYSYLTESKSWDVRKEAIDQFLEAYQLCTHLMYCFS*