AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0361300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0361300 MtrunA17_Chr1g0200221 92.941 85 2 2 1 81 1 85 5.22e-52 160
Msa0361300 MtrunA17_Chr1g0200231 79.762 84 14 1 1 81 1 84 1.28e-43 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0361300 AT2G35820.1 74.359 78 20 0 4 81 8 85 1.99e-39 129
Msa0361300 AT2G35820.2 74.359 78 20 0 4 81 98 175 2.74e-38 128
Msa0361300 AT2G35810.2 71.795 78 22 0 4 81 22 99 4.53e-37 123
Msa0361300 AT2G35810.1 71.795 78 22 0 4 81 22 99 4.53e-37 123
Msa0361300 AT2G35830.2 70.513 78 23 0 4 81 8 85 1.94e-35 119
Msa0361300 AT2G35830.1 67.949 78 20 1 4 81 8 80 7.85e-31 107

Find 71 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAATATCTTGCATTTAA+TGG 0.192051 3_1:-51484824 None:intergenic
ACGGTTAAGCTTGAGAAATT+TGG 0.260726 3_1:-51484480 None:intergenic
AACTAATCATTGATCAATCT+TGG 0.289862 3_1:-51484868 None:intergenic
TCATCGGACGGTGAGGGGTT+TGG 0.318746 3_1:+51483755 Msa0361300:CDS
ACAGACACACTCTTGATGTT+TGG 0.344903 3_1:-51484926 None:intergenic
ACTAATCATTGATCAATCTT+GGG 0.350050 3_1:-51484867 None:intergenic
TGTGTGTAGGAGTAATTTAT+TGG 0.379976 3_1:+51483496 None:intergenic
GGTGGTAATGTTTGGTAGGT+TGG 0.387894 3_1:+51484323 Msa0361300:exon
TTTATGCTGCGCCTCTTGTT+TGG 0.417140 3_1:-51484973 None:intergenic
GACCTGACCGTACTCTTTGA+AGG 0.444255 3_1:-51483723 None:intergenic
GGTCCATTGGTGGTAATGTT+TGG 0.445016 3_1:+51484315 Msa0361300:CDS
CATTGGTGGTAATGTTTGGT+AGG 0.466118 3_1:+51484319 Msa0361300:CDS
ACTCAGTGCCTTGGGTCCAT+TGG 0.468229 3_1:+51484302 Msa0361300:CDS
AGGTCAAGCTGAGCATCGTT+TGG 0.468746 3_1:-51483779 None:intergenic
CAGTGCCTTGGGTCCATTGG+TGG 0.474720 3_1:+51484305 Msa0361300:CDS
CAAGTGTGACTCAGTGCCTT+GGG 0.479051 3_1:+51484294 Msa0361300:CDS
CATAAAGCTCAAATTACTCA+TGG 0.492947 3_1:-51484760 None:intergenic
GCAAGTGTGACTCAGTGCCT+TGG 0.493326 3_1:+51484293 Msa0361300:CDS
TCTATTTAAGTCTGATACAA+TGG 0.496626 3_1:+51484523 Msa0361300:three_prime_UTR
ACCTTAGCCAAGGAATTCCC+AGG 0.496730 3_1:+51483798 Msa0361300:CDS
AAATGAAATCAAGGATGACT+TGG 0.500047 3_1:+51484373 Msa0361300:three_prime_UTR
GAGGCTTCATCGGACGGTGA+GGG 0.503853 3_1:+51483749 Msa0361300:CDS
ATGAAAACCTGGGAATTCCT+TGG 0.506846 3_1:-51483805 None:intergenic
TCATTTGCATCAACAATGGA+TGG 0.507162 3_1:-51484356 None:intergenic
ACCTGGGAATTCCTTGGCTA+AGG 0.513339 3_1:-51483799 None:intergenic
TGTTGATGCAAATGAAATCA+AGG 0.515537 3_1:+51484364 Msa0361300:three_prime_UTR
CTACACCACGTAATCTGATC+GGG 0.523760 3_1:+51483656 Msa0361300:five_prime_UTR
CCACGTAATCTGATCGGGAA+TGG 0.524517 3_1:+51483661 Msa0361300:exon
GTCATTGAGGCTTCATCGGA+CGG 0.541534 3_1:+51483743 Msa0361300:CDS
ATTTGTCCATCACTACATAA+CGG 0.543346 3_1:+51483535 Msa0361300:five_prime_UTR
CTACCAAACATTACCACCAA+TGG 0.543595 3_1:-51484318 None:intergenic
AACCGTGGGACATGGCATGC+TGG 0.544561 3_1:+51484497 Msa0361300:three_prime_UTR
GGTAGTAGGGTTTGAGCGGA+AGG 0.548566 3_1:-51483573 None:intergenic
GATTTCATTTGCATCAACAA+TGG 0.549245 3_1:-51484360 None:intergenic
AGGCTTCATCGGACGGTGAG+GGG 0.550679 3_1:+51483750 Msa0361300:CDS
GAAAACTTGAACATCCTCAA+TGG 0.552500 3_1:-51484444 None:intergenic
TCAGGTCATTGAGGCTTCAT+CGG 0.558495 3_1:+51483739 Msa0361300:CDS
TCAAGCTTAACCGTGGGACA+TGG 0.558912 3_1:+51484489 Msa0361300:three_prime_UTR
CTAATCATTGATCAATCTTG+GGG 0.561772 3_1:-51484866 None:intergenic
ACTTGAACATCCTCAATGGC+AGG 0.565229 3_1:-51484440 None:intergenic
ACTTGCATGATGTGTGATGT+TGG 0.565925 3_1:-51484276 None:intergenic
GACCTTCAAAGAGTACGGTC+AGG 0.570961 3_1:+51483721 Msa0361300:CDS
TTATGTGCCTCCTGCCATTG+AGG 0.572418 3_1:+51484430 Msa0361300:three_prime_UTR
TGAGGCTTCATCGGACGGTG+AGG 0.573414 3_1:+51483748 Msa0361300:CDS
GTCCTTCTTAAAGTTGTGTG+TGG 0.574527 3_1:-51484717 None:intergenic
TACCACACACAACTTTAAGA+AGG 0.575142 3_1:+51484715 Msa0361300:three_prime_UTR
TAATGTTTGGTAGGTTGGAG+TGG 0.578347 3_1:+51484328 Msa0361300:exon
CCGTACTCTTTGAAGGTCGC+CGG 0.579027 3_1:-51483716 None:intergenic
GCTACACCACGTAATCTGAT+CGG 0.584042 3_1:+51483655 Msa0361300:five_prime_UTR
AATGGAGAGTGTGAAGGTGA+AGG 0.586847 3_1:+51483679 Msa0361300:exon
AAAATGCCGTTATGTAGTGA+TGG 0.587866 3_1:-51483541 None:intergenic
GAAGGCAATTGAAGCAACTC+CGG 0.594897 3_1:+51483697 Msa0361300:CDS
CACAACTTTAAGAAGGACAA+TGG 0.594981 3_1:+51484722 Msa0361300:three_prime_UTR
GACCAGCATGCCATGTCCCA+CGG 0.605299 3_1:-51484499 None:intergenic
CCGGCGACCTTCAAAGAGTA+CGG 0.605471 3_1:+51483716 Msa0361300:CDS
AATGAAATCAAGGATGACTT+GGG 0.606584 3_1:+51484374 Msa0361300:three_prime_UTR
TGCATTATGTACCAAACAAG+AGG 0.606614 3_1:+51484962 Msa0361300:three_prime_UTR
CTTTAAGAAGGACAATGGAG+TGG 0.614786 3_1:+51484727 Msa0361300:three_prime_UTR
AAATTTCTCAAGCTTAACCG+TGG 0.618191 3_1:+51484482 Msa0361300:three_prime_UTR
CATTACCACCAATGGACCCA+AGG 0.621913 3_1:-51484310 None:intergenic
ATTGTATCAGACTTAAATAG+AGG 0.622516 3_1:-51484521 None:intergenic
GCTCAGCTTGACCTTAGCCA+AGG 0.638940 3_1:+51483788 Msa0361300:CDS
AATTTCTCAAGCTTAACCGT+GGG 0.640376 3_1:+51484483 Msa0361300:three_prime_UTR
CCATTCCCGATCAGATTACG+TGG 0.645608 3_1:-51483661 None:intergenic
TCAAGGTAGTAGGGTTTGAG+CGG 0.649250 3_1:-51483577 None:intergenic
AGAGTACGGTCAGGTCATTG+AGG 0.671075 3_1:+51483730 Msa0361300:CDS
ATCGGGAATGGAGAGTGTGA+AGG 0.695912 3_1:+51483673 Msa0361300:exon
GTGATGTTGGAAAACTCAAG+TGG 0.699314 3_1:-51484263 None:intergenic
TGAACATCCTCAATGGCAGG+AGG 0.702078 3_1:-51484437 None:intergenic
ACAACAAGACTTGATATACG+TGG 0.723929 3_1:+51483606 Msa0361300:five_prime_UTR
ACAGTCGTGAAGTCACACAG+CGG 0.840888 3_1:+51484401 Msa0361300:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATATCTTGCATTTAA+TGG - chr3_1:51484827-51484846 None:intergenic 15.0%
!! ATATAATATCTTGCATTTAA+TGG - chr3_1:51484827-51484846 None:intergenic 15.0%
!!! TTTATTGTTTTATCAGATCT+TGG + chr3_1:51484062-51484081 Msa0361300:intron 20.0%
!!! TTTATTGTTTTATCAGATCT+TGG + chr3_1:51484062-51484081 Msa0361300:intron 20.0%
!!! ATATCAAGTCTTGTTGTTTT+TGG - chr3_1:51483603-51483622 None:intergenic 25.0%
! ATTAATTCCATTCAGTTACA+GGG - chr3_1:51484202-51484221 None:intergenic 25.0%
! AATTAATTCCATTCAGTTAC+AGG - chr3_1:51484203-51484222 None:intergenic 25.0%
!!! GGAATTAATTTGTGTTTGTT+AGG + chr3_1:51484213-51484232 Msa0361300:intron 25.0%
! ATTGTATCAGACTTAAATAG+AGG - chr3_1:51484524-51484543 None:intergenic 25.0%
! TCTATTTAAGTCTGATACAA+TGG + chr3_1:51484523-51484542 Msa0361300:three_prime_UTR 25.0%
! ACTAATCATTGATCAATCTT+GGG - chr3_1:51484870-51484889 None:intergenic 25.0%
! AACTAATCATTGATCAATCT+TGG - chr3_1:51484871-51484890 None:intergenic 25.0%
!!! ATATCAAGTCTTGTTGTTTT+TGG - chr3_1:51483603-51483622 None:intergenic 25.0%
! ATTAATTCCATTCAGTTACA+GGG - chr3_1:51484202-51484221 None:intergenic 25.0%
! AATTAATTCCATTCAGTTAC+AGG - chr3_1:51484203-51484222 None:intergenic 25.0%
!!! GGAATTAATTTGTGTTTGTT+AGG + chr3_1:51484213-51484232 Msa0361300:intron 25.0%
! ATTGTATCAGACTTAAATAG+AGG - chr3_1:51484524-51484543 None:intergenic 25.0%
! TCTATTTAAGTCTGATACAA+TGG + chr3_1:51484523-51484542 Msa0361300:three_prime_UTR 25.0%
! ACTAATCATTGATCAATCTT+GGG - chr3_1:51484870-51484889 None:intergenic 25.0%
! AACTAATCATTGATCAATCT+TGG - chr3_1:51484871-51484890 None:intergenic 25.0%
ATTTGTCCATCACTACATAA+CGG + chr3_1:51483535-51483554 Msa0361300:five_prime_UTR 30.0%
! GCATCGTTGATAAAAATTGA+AGG - chr3_1:51483835-51483854 None:intergenic 30.0%
! TGTTGATAAGTGTGATTAAG+AGG - chr3_1:51483861-51483880 None:intergenic 30.0%
ATTGTTTAGATTCACAGAGA+TGG + chr3_1:51484038-51484057 Msa0361300:intron 30.0%
TTCTATCAGCTTAATTTAGC+TGG + chr3_1:51484159-51484178 Msa0361300:intron 30.0%
TCTATCAGCTTAATTTAGCT+GGG + chr3_1:51484160-51484179 Msa0361300:intron 30.0%
GATTTCATTTGCATCAACAA+TGG - chr3_1:51484363-51484382 None:intergenic 30.0%
TGTTGATGCAAATGAAATCA+AGG + chr3_1:51484364-51484383 Msa0361300:three_prime_UTR 30.0%
AAATGAAATCAAGGATGACT+TGG + chr3_1:51484373-51484392 Msa0361300:three_prime_UTR 30.0%
AATGAAATCAAGGATGACTT+GGG + chr3_1:51484374-51484393 Msa0361300:three_prime_UTR 30.0%
CATAAAGCTCAAATTACTCA+TGG - chr3_1:51484763-51484782 None:intergenic 30.0%
CTAATCATTGATCAATCTTG+GGG - chr3_1:51484869-51484888 None:intergenic 30.0%
ATTTGTCCATCACTACATAA+CGG + chr3_1:51483535-51483554 Msa0361300:five_prime_UTR 30.0%
! GCATCGTTGATAAAAATTGA+AGG - chr3_1:51483835-51483854 None:intergenic 30.0%
! TGTTGATAAGTGTGATTAAG+AGG - chr3_1:51483861-51483880 None:intergenic 30.0%
ATTGTTTAGATTCACAGAGA+TGG + chr3_1:51484038-51484057 Msa0361300:intron 30.0%
TTCTATCAGCTTAATTTAGC+TGG + chr3_1:51484159-51484178 Msa0361300:intron 30.0%
TCTATCAGCTTAATTTAGCT+GGG + chr3_1:51484160-51484179 Msa0361300:intron 30.0%
GATTTCATTTGCATCAACAA+TGG - chr3_1:51484363-51484382 None:intergenic 30.0%
TGTTGATGCAAATGAAATCA+AGG + chr3_1:51484364-51484383 Msa0361300:three_prime_UTR 30.0%
AAATGAAATCAAGGATGACT+TGG + chr3_1:51484373-51484392 Msa0361300:three_prime_UTR 30.0%
AATGAAATCAAGGATGACTT+GGG + chr3_1:51484374-51484393 Msa0361300:three_prime_UTR 30.0%
CATAAAGCTCAAATTACTCA+TGG - chr3_1:51484763-51484782 None:intergenic 30.0%
CTAATCATTGATCAATCTTG+GGG - chr3_1:51484869-51484888 None:intergenic 30.0%
AAAATGCCGTTATGTAGTGA+TGG - chr3_1:51483544-51483563 None:intergenic 35.0%
! TACATAACGGCATTTTCTCT+TGG + chr3_1:51483548-51483567 Msa0361300:five_prime_UTR 35.0%
!!! TGTTTTTGGTCAAGGTAGTA+GGG - chr3_1:51483589-51483608 None:intergenic 35.0%
!!! TTGTTTTTGGTCAAGGTAGT+AGG - chr3_1:51483590-51483609 None:intergenic 35.0%
!!! AGTCTTGTTGTTTTTGGTCA+AGG - chr3_1:51483597-51483616 None:intergenic 35.0%
ACAACAAGACTTGATATACG+TGG + chr3_1:51483606-51483625 Msa0361300:five_prime_UTR 35.0%
TGAAGGTTGAATGAAAACCT+GGG - chr3_1:51483818-51483837 None:intergenic 35.0%
TTGAAGGTTGAATGAAAACC+TGG - chr3_1:51483819-51483838 None:intergenic 35.0%
GTAGTTGCAATCATCTTGAT+TGG + chr3_1:51483881-51483900 Msa0361300:intron 35.0%
TGTAGTAATTTCTCGCTGTT+TGG + chr3_1:51484086-51484105 Msa0361300:intron 35.0%
GTAGTAATTTCTCGCTGTTT+GGG + chr3_1:51484087-51484106 Msa0361300:intron 35.0%
! GATTTTGAGCACTTTATCTC+AGG - chr3_1:51484123-51484142 None:intergenic 35.0%
TCATTTGCATCAACAATGGA+TGG - chr3_1:51484359-51484378 None:intergenic 35.0%
GAAAACTTGAACATCCTCAA+TGG - chr3_1:51484447-51484466 None:intergenic 35.0%
!! TGAGGATGTTCAAGTTTTCA+AGG + chr3_1:51484448-51484467 Msa0361300:three_prime_UTR 35.0%
!! GTTCAAGTTTTCAAGGTTTC+AGG + chr3_1:51484455-51484474 Msa0361300:three_prime_UTR 35.0%
ACGGTTAAGCTTGAGAAATT+TGG - chr3_1:51484483-51484502 None:intergenic 35.0%
AAATTTCTCAAGCTTAACCG+TGG + chr3_1:51484482-51484501 Msa0361300:three_prime_UTR 35.0%
AATTTCTCAAGCTTAACCGT+GGG + chr3_1:51484483-51484502 Msa0361300:three_prime_UTR 35.0%
AATGTAACACTACACTGGAA+GGG - chr3_1:51484597-51484616 None:intergenic 35.0%
TAATGTAACACTACACTGGA+AGG - chr3_1:51484598-51484617 None:intergenic 35.0%
CATGTAATGTAACACTACAC+TGG - chr3_1:51484602-51484621 None:intergenic 35.0%
! AGAGCATCACATATGAATTC+AGG - chr3_1:51484627-51484646 None:intergenic 35.0%
!!! TGGTTTTGTATTCTGTGTTC+AGG + chr3_1:51484682-51484701 Msa0361300:intron 35.0%
! TACCACACACAACTTTAAGA+AGG + chr3_1:51484715-51484734 Msa0361300:three_prime_UTR 35.0%
! CACAACTTTAAGAAGGACAA+TGG + chr3_1:51484722-51484741 Msa0361300:three_prime_UTR 35.0%
TGCATTATGTACCAAACAAG+AGG + chr3_1:51484962-51484981 Msa0361300:three_prime_UTR 35.0%
AAAATGCCGTTATGTAGTGA+TGG - chr3_1:51483544-51483563 None:intergenic 35.0%
! TACATAACGGCATTTTCTCT+TGG + chr3_1:51483548-51483567 Msa0361300:five_prime_UTR 35.0%
!!! TGTTTTTGGTCAAGGTAGTA+GGG - chr3_1:51483589-51483608 None:intergenic 35.0%
!!! TTGTTTTTGGTCAAGGTAGT+AGG - chr3_1:51483590-51483609 None:intergenic 35.0%
!!! AGTCTTGTTGTTTTTGGTCA+AGG - chr3_1:51483597-51483616 None:intergenic 35.0%
ACAACAAGACTTGATATACG+TGG + chr3_1:51483606-51483625 Msa0361300:five_prime_UTR 35.0%
TGAAGGTTGAATGAAAACCT+GGG - chr3_1:51483818-51483837 None:intergenic 35.0%
TTGAAGGTTGAATGAAAACC+TGG - chr3_1:51483819-51483838 None:intergenic 35.0%
GTAGTTGCAATCATCTTGAT+TGG + chr3_1:51483881-51483900 Msa0361300:intron 35.0%
TGTAGTAATTTCTCGCTGTT+TGG + chr3_1:51484086-51484105 Msa0361300:intron 35.0%
GTAGTAATTTCTCGCTGTTT+GGG + chr3_1:51484087-51484106 Msa0361300:intron 35.0%
! GATTTTGAGCACTTTATCTC+AGG - chr3_1:51484123-51484142 None:intergenic 35.0%
TCATTTGCATCAACAATGGA+TGG - chr3_1:51484359-51484378 None:intergenic 35.0%
GAAAACTTGAACATCCTCAA+TGG - chr3_1:51484447-51484466 None:intergenic 35.0%
!! TGAGGATGTTCAAGTTTTCA+AGG + chr3_1:51484448-51484467 Msa0361300:three_prime_UTR 35.0%
!! GTTCAAGTTTTCAAGGTTTC+AGG + chr3_1:51484455-51484474 Msa0361300:three_prime_UTR 35.0%
ACGGTTAAGCTTGAGAAATT+TGG - chr3_1:51484483-51484502 None:intergenic 35.0%
AAATTTCTCAAGCTTAACCG+TGG + chr3_1:51484482-51484501 Msa0361300:three_prime_UTR 35.0%
AATTTCTCAAGCTTAACCGT+GGG + chr3_1:51484483-51484502 Msa0361300:three_prime_UTR 35.0%
AATGTAACACTACACTGGAA+GGG - chr3_1:51484597-51484616 None:intergenic 35.0%
TAATGTAACACTACACTGGA+AGG - chr3_1:51484598-51484617 None:intergenic 35.0%
CATGTAATGTAACACTACAC+TGG - chr3_1:51484602-51484621 None:intergenic 35.0%
! AGAGCATCACATATGAATTC+AGG - chr3_1:51484627-51484646 None:intergenic 35.0%
!!! TGGTTTTGTATTCTGTGTTC+AGG + chr3_1:51484682-51484701 Msa0361300:intron 35.0%
! TACCACACACAACTTTAAGA+AGG + chr3_1:51484715-51484734 Msa0361300:three_prime_UTR 35.0%
! CACAACTTTAAGAAGGACAA+TGG + chr3_1:51484722-51484741 Msa0361300:three_prime_UTR 35.0%
TGCATTATGTACCAAACAAG+AGG + chr3_1:51484962-51484981 Msa0361300:three_prime_UTR 35.0%
ATGAAAACCTGGGAATTCCT+TGG - chr3_1:51483808-51483827 None:intergenic 40.0%
! GATTGGTTAACCGCTTGTAT+TGG + chr3_1:51483898-51483917 Msa0361300:intron 40.0%
GTGATGTTGGAAAACTCAAG+TGG - chr3_1:51484266-51484285 None:intergenic 40.0%
ACTTGCATGATGTGTGATGT+TGG - chr3_1:51484279-51484298 None:intergenic 40.0%
CTACCAAACATTACCACCAA+TGG - chr3_1:51484321-51484340 None:intergenic 40.0%
! CATTGGTGGTAATGTTTGGT+AGG + chr3_1:51484319-51484338 Msa0361300:CDS 40.0%
! TAATGTTTGGTAGGTTGGAG+TGG + chr3_1:51484328-51484347 Msa0361300:exon 40.0%
CAGTCGCTGTGATTAATGTA+TGG + chr3_1:51484662-51484681 Msa0361300:intron 40.0%
GTCCTTCTTAAAGTTGTGTG+TGG - chr3_1:51484720-51484739 None:intergenic 40.0%
! CTTTAAGAAGGACAATGGAG+TGG + chr3_1:51484727-51484746 Msa0361300:three_prime_UTR 40.0%
ACAGACACACTCTTGATGTT+TGG - chr3_1:51484929-51484948 None:intergenic 40.0%
ATGAAAACCTGGGAATTCCT+TGG - chr3_1:51483808-51483827 None:intergenic 40.0%
! GATTGGTTAACCGCTTGTAT+TGG + chr3_1:51483898-51483917 Msa0361300:intron 40.0%
GTGATGTTGGAAAACTCAAG+TGG - chr3_1:51484266-51484285 None:intergenic 40.0%
ACTTGCATGATGTGTGATGT+TGG - chr3_1:51484279-51484298 None:intergenic 40.0%
CTACCAAACATTACCACCAA+TGG - chr3_1:51484321-51484340 None:intergenic 40.0%
! CATTGGTGGTAATGTTTGGT+AGG + chr3_1:51484319-51484338 Msa0361300:CDS 40.0%
! TAATGTTTGGTAGGTTGGAG+TGG + chr3_1:51484328-51484347 Msa0361300:exon 40.0%
CAGTCGCTGTGATTAATGTA+TGG + chr3_1:51484662-51484681 Msa0361300:intron 40.0%
GTCCTTCTTAAAGTTGTGTG+TGG - chr3_1:51484720-51484739 None:intergenic 40.0%
! CTTTAAGAAGGACAATGGAG+TGG + chr3_1:51484727-51484746 Msa0361300:three_prime_UTR 40.0%
ACAGACACACTCTTGATGTT+TGG - chr3_1:51484929-51484948 None:intergenic 40.0%
TCAAGGTAGTAGGGTTTGAG+CGG - chr3_1:51483580-51483599 None:intergenic 45.0%
GCTACACCACGTAATCTGAT+CGG + chr3_1:51483655-51483674 Msa0361300:five_prime_UTR 45.0%
CTACACCACGTAATCTGATC+GGG + chr3_1:51483656-51483675 Msa0361300:five_prime_UTR 45.0%
! AATGGAGAGTGTGAAGGTGA+AGG + chr3_1:51483679-51483698 Msa0361300:exon 45.0%
GAAGGCAATTGAAGCAACTC+CGG + chr3_1:51483697-51483716 Msa0361300:CDS 45.0%
TCAGGTCATTGAGGCTTCAT+CGG + chr3_1:51483739-51483758 Msa0361300:CDS 45.0%
! GTTAACCGCTTGTATTGGTC+CGG + chr3_1:51483903-51483922 Msa0361300:intron 45.0%
TTCGATTCTGCAGTCTGAGT+GGG + chr3_1:51483978-51483997 Msa0361300:intron 45.0%
AGCTATGCCCTGTAACTGAA+TGG + chr3_1:51484192-51484211 Msa0361300:intron 45.0%
! GGTCCATTGGTGGTAATGTT+TGG + chr3_1:51484315-51484334 Msa0361300:CDS 45.0%
! GGTGGTAATGTTTGGTAGGT+TGG + chr3_1:51484323-51484342 Msa0361300:exon 45.0%
ACTTGAACATCCTCAATGGC+AGG - chr3_1:51484443-51484462 None:intergenic 45.0%
TAACACTACACTGGAAGGGA+AGG - chr3_1:51484593-51484612 None:intergenic 45.0%
TTTATGCTGCGCCTCTTGTT+TGG - chr3_1:51484976-51484995 None:intergenic 45.0%
TCAAGGTAGTAGGGTTTGAG+CGG - chr3_1:51483580-51483599 None:intergenic 45.0%
GCTACACCACGTAATCTGAT+CGG + chr3_1:51483655-51483674 Msa0361300:five_prime_UTR 45.0%
CTACACCACGTAATCTGATC+GGG + chr3_1:51483656-51483675 Msa0361300:five_prime_UTR 45.0%
! AATGGAGAGTGTGAAGGTGA+AGG + chr3_1:51483679-51483698 Msa0361300:exon 45.0%
GAAGGCAATTGAAGCAACTC+CGG + chr3_1:51483697-51483716 Msa0361300:CDS 45.0%
TCAGGTCATTGAGGCTTCAT+CGG + chr3_1:51483739-51483758 Msa0361300:CDS 45.0%
! GTTAACCGCTTGTATTGGTC+CGG + chr3_1:51483903-51483922 Msa0361300:intron 45.0%
TTCGATTCTGCAGTCTGAGT+GGG + chr3_1:51483978-51483997 Msa0361300:intron 45.0%
AGCTATGCCCTGTAACTGAA+TGG + chr3_1:51484192-51484211 Msa0361300:intron 45.0%
! GGTCCATTGGTGGTAATGTT+TGG + chr3_1:51484315-51484334 Msa0361300:CDS 45.0%
! GGTGGTAATGTTTGGTAGGT+TGG + chr3_1:51484323-51484342 Msa0361300:exon 45.0%
ACTTGAACATCCTCAATGGC+AGG - chr3_1:51484443-51484462 None:intergenic 45.0%
TAACACTACACTGGAAGGGA+AGG - chr3_1:51484593-51484612 None:intergenic 45.0%
TTTATGCTGCGCCTCTTGTT+TGG - chr3_1:51484976-51484995 None:intergenic 45.0%
CCATTCCCGATCAGATTACG+TGG - chr3_1:51483664-51483683 None:intergenic 50.0%
CCACGTAATCTGATCGGGAA+TGG + chr3_1:51483661-51483680 Msa0361300:exon 50.0%
! ATCGGGAATGGAGAGTGTGA+AGG + chr3_1:51483673-51483692 Msa0361300:exon 50.0%
! GACCTTCAAAGAGTACGGTC+AGG + chr3_1:51483721-51483740 Msa0361300:CDS 50.0%
GACCTGACCGTACTCTTTGA+AGG - chr3_1:51483726-51483745 None:intergenic 50.0%
AGAGTACGGTCAGGTCATTG+AGG + chr3_1:51483730-51483749 Msa0361300:CDS 50.0%
GTCATTGAGGCTTCATCGGA+CGG + chr3_1:51483743-51483762 Msa0361300:CDS 50.0%
! AGGTCAAGCTGAGCATCGTT+TGG - chr3_1:51483782-51483801 None:intergenic 50.0%
ACCTTAGCCAAGGAATTCCC+AGG + chr3_1:51483798-51483817 Msa0361300:CDS 50.0%
ACCTGGGAATTCCTTGGCTA+AGG - chr3_1:51483802-51483821 None:intergenic 50.0%
TACCACCGGACCAATACAAG+CGG - chr3_1:51483911-51483930 None:intergenic 50.0%
AGGTTCCACGTCAATACCAC+CGG - chr3_1:51483925-51483944 None:intergenic 50.0%
AGTGAGTGTGCTCCTCTTCA+AGG + chr3_1:51483948-51483967 Msa0361300:intron 50.0%
TCGAACGTGAGACCTTGAAG+AGG - chr3_1:51483963-51483982 None:intergenic 50.0%
GTTCGATTCTGCAGTCTGAG+TGG + chr3_1:51483977-51483996 Msa0361300:intron 50.0%
CAAGTGTGACTCAGTGCCTT+GGG + chr3_1:51484294-51484313 Msa0361300:CDS 50.0%
CATTACCACCAATGGACCCA+AGG - chr3_1:51484313-51484332 None:intergenic 50.0%
ACAGTCGTGAAGTCACACAG+CGG + chr3_1:51484401-51484420 Msa0361300:three_prime_UTR 50.0%
TTATGTGCCTCCTGCCATTG+AGG + chr3_1:51484430-51484449 Msa0361300:three_prime_UTR 50.0%
TGAACATCCTCAATGGCAGG+AGG - chr3_1:51484440-51484459 None:intergenic 50.0%
TCAAGCTTAACCGTGGGACA+TGG + chr3_1:51484489-51484508 Msa0361300:three_prime_UTR 50.0%
CCATTCCCGATCAGATTACG+TGG - chr3_1:51483664-51483683 None:intergenic 50.0%
CCACGTAATCTGATCGGGAA+TGG + chr3_1:51483661-51483680 Msa0361300:exon 50.0%
! ATCGGGAATGGAGAGTGTGA+AGG + chr3_1:51483673-51483692 Msa0361300:exon 50.0%
! GACCTTCAAAGAGTACGGTC+AGG + chr3_1:51483721-51483740 Msa0361300:CDS 50.0%
GACCTGACCGTACTCTTTGA+AGG - chr3_1:51483726-51483745 None:intergenic 50.0%
AGAGTACGGTCAGGTCATTG+AGG + chr3_1:51483730-51483749 Msa0361300:CDS 50.0%
GTCATTGAGGCTTCATCGGA+CGG + chr3_1:51483743-51483762 Msa0361300:CDS 50.0%
! AGGTCAAGCTGAGCATCGTT+TGG - chr3_1:51483782-51483801 None:intergenic 50.0%
ACCTTAGCCAAGGAATTCCC+AGG + chr3_1:51483798-51483817 Msa0361300:CDS 50.0%
ACCTGGGAATTCCTTGGCTA+AGG - chr3_1:51483802-51483821 None:intergenic 50.0%
TACCACCGGACCAATACAAG+CGG - chr3_1:51483911-51483930 None:intergenic 50.0%
AGGTTCCACGTCAATACCAC+CGG - chr3_1:51483925-51483944 None:intergenic 50.0%
AGTGAGTGTGCTCCTCTTCA+AGG + chr3_1:51483948-51483967 Msa0361300:intron 50.0%
TCGAACGTGAGACCTTGAAG+AGG - chr3_1:51483963-51483982 None:intergenic 50.0%
GTTCGATTCTGCAGTCTGAG+TGG + chr3_1:51483977-51483996 Msa0361300:intron 50.0%
CAAGTGTGACTCAGTGCCTT+GGG + chr3_1:51484294-51484313 Msa0361300:CDS 50.0%
CATTACCACCAATGGACCCA+AGG - chr3_1:51484313-51484332 None:intergenic 50.0%
ACAGTCGTGAAGTCACACAG+CGG + chr3_1:51484401-51484420 Msa0361300:three_prime_UTR 50.0%
TTATGTGCCTCCTGCCATTG+AGG + chr3_1:51484430-51484449 Msa0361300:three_prime_UTR 50.0%
TGAACATCCTCAATGGCAGG+AGG - chr3_1:51484440-51484459 None:intergenic 50.0%
TCAAGCTTAACCGTGGGACA+TGG + chr3_1:51484489-51484508 Msa0361300:three_prime_UTR 50.0%
GGTAGTAGGGTTTGAGCGGA+AGG - chr3_1:51483576-51483595 None:intergenic 55.0%
! CCGTACTCTTTGAAGGTCGC+CGG - chr3_1:51483719-51483738 None:intergenic 55.0%
CCGGCGACCTTCAAAGAGTA+CGG + chr3_1:51483716-51483735 Msa0361300:CDS 55.0%
GCTCAGCTTGACCTTAGCCA+AGG + chr3_1:51483788-51483807 Msa0361300:CDS 55.0%
! AACCGCTTGTATTGGTCCGG+TGG + chr3_1:51483906-51483925 Msa0361300:intron 55.0%
!! TTGGTCCGGTGGTATTGACG+TGG + chr3_1:51483917-51483936 Msa0361300:intron 55.0%
! AGAGGAGCACACTCACTCTC+AGG - chr3_1:51483945-51483964 None:intergenic 55.0%
GCAAGTGTGACTCAGTGCCT+TGG + chr3_1:51484293-51484312 Msa0361300:CDS 55.0%
ACTCAGTGCCTTGGGTCCAT+TGG + chr3_1:51484302-51484321 Msa0361300:CDS 55.0%
GGTAGTAGGGTTTGAGCGGA+AGG - chr3_1:51483576-51483595 None:intergenic 55.0%
! CCGTACTCTTTGAAGGTCGC+CGG - chr3_1:51483719-51483738 None:intergenic 55.0%
CCGGCGACCTTCAAAGAGTA+CGG + chr3_1:51483716-51483735 Msa0361300:CDS 55.0%
GCTCAGCTTGACCTTAGCCA+AGG + chr3_1:51483788-51483807 Msa0361300:CDS 55.0%
! AACCGCTTGTATTGGTCCGG+TGG + chr3_1:51483906-51483925 Msa0361300:intron 55.0%
!! TTGGTCCGGTGGTATTGACG+TGG + chr3_1:51483917-51483936 Msa0361300:intron 55.0%
! AGAGGAGCACACTCACTCTC+AGG - chr3_1:51483945-51483964 None:intergenic 55.0%
GCAAGTGTGACTCAGTGCCT+TGG + chr3_1:51484293-51484312 Msa0361300:CDS 55.0%
ACTCAGTGCCTTGGGTCCAT+TGG + chr3_1:51484302-51484321 Msa0361300:CDS 55.0%
TGAGGCTTCATCGGACGGTG+AGG + chr3_1:51483748-51483767 Msa0361300:CDS 60.0%
GAGGCTTCATCGGACGGTGA+GGG + chr3_1:51483749-51483768 Msa0361300:CDS 60.0%
AGGCTTCATCGGACGGTGAG+GGG + chr3_1:51483750-51483769 Msa0361300:CDS 60.0%
TCATCGGACGGTGAGGGGTT+TGG + chr3_1:51483755-51483774 Msa0361300:CDS 60.0%
CAGTGCCTTGGGTCCATTGG+TGG + chr3_1:51484305-51484324 Msa0361300:CDS 60.0%
AACCGTGGGACATGGCATGC+TGG + chr3_1:51484497-51484516 Msa0361300:three_prime_UTR 60.0%
GACCAGCATGCCATGTCCCA+CGG - chr3_1:51484502-51484521 None:intergenic 60.0%
TGAGGCTTCATCGGACGGTG+AGG + chr3_1:51483748-51483767 Msa0361300:CDS 60.0%
GAGGCTTCATCGGACGGTGA+GGG + chr3_1:51483749-51483768 Msa0361300:CDS 60.0%
AGGCTTCATCGGACGGTGAG+GGG + chr3_1:51483750-51483769 Msa0361300:CDS 60.0%
TCATCGGACGGTGAGGGGTT+TGG + chr3_1:51483755-51483774 Msa0361300:CDS 60.0%
CAGTGCCTTGGGTCCATTGG+TGG + chr3_1:51484305-51484324 Msa0361300:CDS 60.0%
AACCGTGGGACATGGCATGC+TGG + chr3_1:51484497-51484516 Msa0361300:three_prime_UTR 60.0%
GACCAGCATGCCATGTCCCA+CGG - chr3_1:51484502-51484521 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 51483500 51485010 51483500 ID=Msa0361300;Name=Msa0361300
chr3_1 mRNA 51483500 51485010 51483500 ID=Msa0361300-mRNA-1;Parent=Msa0361300;Name=Msa0361300-mRNA-1;_AED=0.06;_eAED=0.06;_QI=180|1|1|1|1|0.66|3|544|81
chr3_1 exon 51483500 51483819 51483500 ID=Msa0361300-mRNA-1:exon:9147;Parent=Msa0361300-mRNA-1
chr3_1 exon 51484235 51484577 51484235 ID=Msa0361300-mRNA-1:exon:9148;Parent=Msa0361300-mRNA-1
chr3_1 exon 51484704 51485010 51484704 ID=Msa0361300-mRNA-1:exon:9149;Parent=Msa0361300-mRNA-1
chr3_1 five_prime_UTR 51483500 51483679 51483500 ID=Msa0361300-mRNA-1:five_prime_utr;Parent=Msa0361300-mRNA-1
chr3_1 CDS 51483680 51483819 51483680 ID=Msa0361300-mRNA-1:cds;Parent=Msa0361300-mRNA-1
chr3_1 CDS 51484235 51484340 51484235 ID=Msa0361300-mRNA-1:cds;Parent=Msa0361300-mRNA-1
chr3_1 three_prime_UTR 51484341 51484577 51484341 ID=Msa0361300-mRNA-1:three_prime_utr;Parent=Msa0361300-mRNA-1
chr3_1 three_prime_UTR 51484704 51485010 51484704 ID=Msa0361300-mRNA-1:three_prime_utr;Parent=Msa0361300-mRNA-1
Gene Sequence

>Msa0361300

ATGGAGAGTGTGAAGGTGAAGGCAATTGAAGCAACTCCGGCGACCTTCAAAGAGTACGGTCAGGTCATTGAGGCTTCATCGGACGGTGAGGGGTTTGGTCCAAACGATGCTCAGCTTGACCTTAGCCAAGGAATTCCCAGGTTCTACATTATGCATCTTGAAAATCGTCCACTTGAGTTTTCCAACATCACACATCATGCAAGTGTGACTCAGTGCCTTGGGTCCATTGGTGGTAATGTTTGGTAG
Protein sequence

>Msa0361300

MESVKVKAIEATPATFKEYGQVIEASSDGEGFGPNDAQLDLSQGIPRFYIMHLENRPLEFSNITHHASVTQCLGSIGGNVW*