AlfalfaGEDB | Msa0364480

M. sativa cultivar zhongmu-4 / Msa0364480

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0364480	XP_003600455.1	74.016	127	32	1	10	136	156	281	5.21e-51	184
Msa0364480	XP_003600455.1	84.859	284	39	2	163	445	1	281	1.80e-172	494

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0364480	sp\|Q8L3W1\|VRN1_ARATH	29.032	341	171	13	170	445	1	335	2.48e-28	117
Msa0364480	sp\|Q8L3W1\|VRN1_ARATH	31.008	129	82	4	12	137	212	336	3.90e-12	70.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0364480	G8A2Z2	74.016	127	32	1	10	136	156	281	2.49e-51	184
Msa0364480	G8A2Z2	84.859	284	39	2	163	445	1	281	8.59e-173	494

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TFs/TRs:

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Gene ID	Type	Classification
Msa0364480	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0364480	Msa0456120	0.834461	2.957943e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0364480	MtrunA17_Chr3g0103181	75.352	284	64	4	163	445	1	279	6.99e-149	424
Msa0364480	MtrunA17_Chr3g0103181	67.544	114	36	1	23	136	167	279	3.24e-42	150
Msa0364480	MtrunA17_Chr3g0103191	80.478	251	48	1	163	412	1	251	1.19e-144	412
Msa0364480	MtrunA17_Chr3g0103191	72.340	94	26	0	10	103	158	251	4.27e-39	141
Msa0364480	MtrunA17_Chr3g0103201	82.273	220	38	1	227	445	1	220	3.13e-129	372
Msa0364480	MtrunA17_Chr3g0103201	74.016	127	32	1	10	136	95	220	4.22e-56	185
Msa0364480	MtrunA17_Chr3g0103171	70.611	262	73	3	187	445	5	265	2.06e-127	369
Msa0364480	MtrunA17_Chr3g0103171	58.462	130	53	1	8	136	136	265	5.74e-42	149
Msa0364480	MtrunA17_Chr3g0103151	76.543	162	36	2	163	323	1	161	5.86e-84	254
Msa0364480	MtrunA17_Chr3g0103161	68.595	121	38	0	324	444	2	122	1.64e-52	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0364480	AT3G18990.1	29.032	341	171	13	170	445	1	335	2.52e-29	117
Msa0364480	AT3G18990.1	31.008	129	82	4	12	137	212	336	3.96e-13	70.9
Msa0364480	AT3G18990.2	29.032	341	171	13	170	445	1	335	9.62e-29	116
Msa0364480	AT3G18990.2	31.008	129	82	4	12	137	212	336	4.83e-13	70.9
Msa0364480	AT1G49475.1	37.162	148	79	3	173	307	33	179	2.29e-18	83.2
Msa0364480	AT4G01580.1	34.058	138	83	3	173	308	29	160	1.24e-15	75.5
Msa0364480	AT3G18960.2	39.000	100	59	1	173	270	29	128	5.32e-15	73.9
Msa0364480	AT3G18960.1	39.000	100	59	1	173	270	29	128	6.21e-15	73.9
Msa0364480	AT1G49480.3	30.233	129	83	4	12	137	97	221	7.42e-14	71.2
Msa0364480	AT1G49480.3	31.538	130	77	5	322	445	97	220	3.09e-12	66.6

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Find 74 sgRNAs with CRISPR-Local

Find 379 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTCTAACTTTCTTGTG+TGG	0.625711	3_1:+57548111	None:intergenic
AAAGAACTTCATCAATGAAT+CGG	0.459762	3_1:-57547932	Msa0364480:CDS
AAAGAACTTCATCAATGAAT+TGG	0.387908	3_1:-57554200	Msa0364480:CDS
AAATGGCCGTCGCCTTTCCG+CGG	0.508666	3_1:-57547565	Msa0364480:CDS
AAATGGTTTCTATCTATCGG+CGG	0.576380	3_1:-57553847	Msa0364480:CDS
AAGAATGTGATGCTGCTGAT+TGG	0.482287	3_1:-57547633	Msa0364480:CDS
AAGTATGTGTACATCAAACT+TGG	0.555627	3_1:+57548290	None:intergenic
AAGTTTGATGTACACATACT+TGG	0.471543	3_1:-57548288	Msa0364480:CDS
AATCTATTTCTACCGCATTC+TGG	0.299695	3_1:+57548265	None:intergenic
AATGGCCGTCGCCTTTCCGC+GGG	0.545483	3_1:-57547564	Msa0364480:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATACATAGAATTGCAGGAA+TGG	+	chr3_1:57547667-57547686	None:intergenic	30.0%
TCGTAAAACAATTGAAGACA+TGG	-	chr3_1:57548931-57548950	Msa0364480:intron	30.0%
AAAACAATTGAAGACATGGA+AGG	-	chr3_1:57548935-57548954	Msa0364480:intron	30.0%
AAACAATTGAAGACATGGAA+GGG	-	chr3_1:57548936-57548955	Msa0364480:intron	30.0%
GAAATGGGAATTGAGTATTA+AGG	-	chr3_1:57548958-57548977	Msa0364480:intron	30.0%
ATACAATATCTCGTATACCT+AGG	+	chr3_1:57549006-57549025	None:intergenic	30.0%
AGGGTTTGGAAAATCAAAAT+TGG	-	chr3_1:57549522-57549541	Msa0364480:intron	30.0%
GGGTTTGGAAAATCAAAATT+GGG	-	chr3_1:57549523-57549542	Msa0364480:intron	30.0%
GGTTTGGAAAATCAAAATTG+GGG	-	chr3_1:57549524-57549543	Msa0364480:intron	30.0%
TTTAAGTAGGAATGAGCTAT+TGG	-	chr3_1:57549914-57549933	Msa0364480:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	57547461	57554271	57547461	ID=Msa0364480;Name=Msa0364480
chr3_1	mRNA	57547461	57554271	57547461	ID=Msa0364480-mRNA-1;Parent=Msa0364480;Name=Msa0364480-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0.2\|0\|1\|0.8\|0.66\|6\|0\|445
chr3_1	exon	57554098	57554271	57554098	ID=Msa0364480-mRNA-1:exon:10815;Parent=Msa0364480-mRNA-1
chr3_1	exon	57553728	57553979	57553728	ID=Msa0364480-mRNA-1:exon:10814;Parent=Msa0364480-mRNA-1
chr3_1	exon	57548799	57548930	57548799	ID=Msa0364480-mRNA-1:exon:10813;Parent=Msa0364480-mRNA-1
chr3_1	exon	57548115	57548514	57548115	ID=Msa0364480-mRNA-1:exon:10812;Parent=Msa0364480-mRNA-1
chr3_1	exon	57547830	57547975	57547830	ID=Msa0364480-mRNA-1:exon:10811;Parent=Msa0364480-mRNA-1
chr3_1	exon	57547461	57547694	57547461	ID=Msa0364480-mRNA-1:exon:10810;Parent=Msa0364480-mRNA-1
chr3_1	CDS	57554098	57554271	57554098	ID=Msa0364480-mRNA-1:cds;Parent=Msa0364480-mRNA-1
chr3_1	CDS	57553728	57553979	57553728	ID=Msa0364480-mRNA-1:cds;Parent=Msa0364480-mRNA-1
chr3_1	CDS	57548799	57548930	57548799	ID=Msa0364480-mRNA-1:cds;Parent=Msa0364480-mRNA-1
chr3_1	CDS	57548115	57548514	57548115	ID=Msa0364480-mRNA-1:cds;Parent=Msa0364480-mRNA-1
chr3_1	CDS	57547830	57547975	57547830	ID=Msa0364480-mRNA-1:cds;Parent=Msa0364480-mRNA-1
chr3_1	CDS	57547461	57547694	57547461	ID=Msa0364480-mRNA-1:cds;Parent=Msa0364480-mRNA-1

Gene Sequence

>Msa0364480

ATGTTTTATATTGCACACATATACGCAGGTGATCATGAGAAGACTACTAAAAGAACTTCATCAATGAATTGGCCAAAAGATGCTAGAGCTCAGGAAGTTGCTCAAAATTTCATCTCAACCAATCCTTTTTTCACAATTCTTGTAAAACCTCACCATACAGTAGATAGTAAGCTGTATGTAACTGCAGATTTAAAAGGAGTTATTGAGAACAAAGAGGAGTATGTGATTCTGCAGATTGGGAAAAGATCATGGAATGTGAAATTGATCTGTTCTAACGAGGCTAAAAATGGTTTCTATCTATCGGCGGGTTGGTACTTGTTTGCAAGCGAAAGTGAACTGCAACCAGGAGATGTTTGTGTCTTTGAACTGATAAAAAAGAAAGATTTCGTTTTTAAAGTTCATGTCTTCAAAAAAGGGACCGTTAATTATAGAAGAGTGACACACAATCAAAACATCTCATTATCTCCACTTCACACTCTTTCATCCATGGCTAGAGGAAATTCTATGCTTCCCATCCGTTTCTTCAAAATCATCCTTCAAACTAATCTTCATAGGATTCAAATACCAAGAAAGTTTACAAAGAGACATGGAGTTGGTCTATCAAATCCAGTGGTGATAAAGACTCCTGATGACACTAAATGGAAAATTTATTGGAAAAAAATCAATGGTGAGATTTGGTTTGAAAGGGGTTGGAAATGTTTTACTGAAAATTACTCTCTTGGACATGGCTGTTTGGTGGTGTTTGAATATAAAGGAACTTCCAAGTTTGATGTACACATACTTGGCCAGAATGCGGTAGAAATAGATTATTCATCAAGCGACACTGATGATGAAAATGACAATGTTGATCATAGTGATGATGATGAATCAGTTGAGTTTTTGGATGAATGGCTGAATCGGAAGAAGGCTTCAAAAAGATTACCTTTTGTTTCTCCTCGACCACACAAGAAAGTTAGAGGTGATCATGAGAAGAGTGCTAAAAGAACTTCATCAATGAATCGGCCAAAGGATGTTAGAGCTCAGGAAGTAGCTCAAAATTTCACCTCATGCAATCCTTTCTTCACAGTTCTCATACAGCCCAATCATCTGGAAGCAAATCAACTGAATGTACCAAATTTTAAAGGGGTTATTGAGAACAAGGAGAAGAATGTGATGCTGCTGATTGGGAAAAGATCATGGAATGTGAAACTACTTCGTTCTTATGAGAGCAAAAATGGCCGTCGCCTTTCCGCGGGCTGGTACTTATTTGCAAGAGAAAGTGGACTGAAATCAGGAGATGTTTGTGTCTTTGAATTGATAAACAAGAAAGATTTGGTTTTTAAAGTTCATGTTTTCTAA

Protein sequence

>Msa0364480

MFYIAHIYAGDHEKTTKRTSSMNWPKDARAQEVAQNFISTNPFFTILVKPHHTVDSKLYVTADLKGVIENKEEYVILQIGKRSWNVKLICSNEAKNGFYLSAGWYLFASESELQPGDVCVFELIKKKDFVFKVHVFKKGTVNYRRVTHNQNISLSPLHTLSSMARGNSMLPIRFFKIILQTNLHRIQIPRKFTKRHGVGLSNPVVIKTPDDTKWKIYWKKINGEIWFERGWKCFTENYSLGHGCLVVFEYKGTSKFDVHILGQNAVEIDYSSSDTDDENDNVDHSDDDESVEFLDEWLNRKKASKRLPFVSPRPHKKVRGDHEKSAKRTSSMNRPKDVRAQEVAQNFTSCNPFFTVLIQPNHLEANQLNVPNFKGVIENKEKNVMLLIGKRSWNVKLLRSYESKNGRRLSAGWYLFARESGLKSGDVCVFELINKKDLVFKVHVF*