AlfalfaGEDB | Msa0456120

M. sativa cultivar zhongmu-4 / Msa0456120

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456120	XP_003600455.1	79.310	261	52	2	1	260	22	281	9.57e-145	417

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456120	sp\|Q8L3W1\|VRN1_ARATH	26.250	320	173	8	1	261	21	336	2.35e-30	119

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456120	G8A2Z2	79.310	261	52	2	1	260	22	281	4.57e-145	417

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TFs/TRs:

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Gene ID	Type	Classification
Msa0456120	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0364480	Msa0456120	0.834461	2.957943e-56	-8.615850e-47
Msa0456120	Msa0503770	0.883030	6.333305e-71	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456120	MtrunA17_Chr3g0103191	81.579	228	40	2	1	227	25	251	2.06e-134	379
Msa0456120	MtrunA17_Chr3g0103181	71.923	260	68	3	1	260	25	279	2.66e-131	372
Msa0456120	MtrunA17_Chr3g0103171	70.115	261	77	1	1	260	5	265	1.01e-130	370
Msa0456120	MtrunA17_Chr3g0103201	76.923	221	49	2	41	260	1	220	9.88e-119	338
Msa0456120	MtrunA17_Chr3g0103151	80.292	137	27	0	1	137	25	161	1.25e-77	232
Msa0456120	MtrunA17_Chr3g0103161	66.935	124	40	1	138	261	2	124	1.84e-52	167
Msa0456120	MtrunA17_Chr1g0163071	36.177	293	147	9	2	261	2	287	8.87e-51	169
Msa0456120	MtrunA17_Chr8g0390841	33.220	295	150	7	1	263	54	333	4.85e-46	161
Msa0456120	MtrunA17_Chr8g0390841	48.438	64	30	2	199	261	457	518	2.62e-11	63.5
Msa0456120	MtrunA17_Chr1g0208771	32.313	294	151	7	1	261	12	290	3.87e-45	157

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0456120	AT3G18990.1	26.250	320	173	8	1	261	21	336	2.38e-31	119
Msa0456120	AT3G18990.2	26.250	320	173	8	1	261	21	336	4.10e-31	119
Msa0456120	AT1G49475.1	40.000	105	61	2	1	105	49	151	5.89e-17	77.4
Msa0456120	AT4G01580.1	34.677	124	71	2	1	122	45	160	1.73e-15	73.2
Msa0456120	AT4G33280.1	25.087	287	182	10	2	261	39	319	1.37e-14	73.2
Msa0456120	AT3G18960.2	40.476	84	50	0	1	84	45	128	1.53e-14	70.9
Msa0456120	AT3G18960.3	40.476	84	50	0	1	84	8	91	1.66e-14	70.1
Msa0456120	AT3G18960.1	40.476	84	50	0	1	84	45	128	1.72e-14	70.9
Msa0456120	AT1G49480.3	29.457	129	84	4	136	261	97	221	1.46e-13	68.6
Msa0456120	AT1G49480.1	29.457	129	84	4	136	261	97	221	1.46e-13	68.6

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Find 47 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTTTAACTTTCTTATG+TGG	0.464229	3_3:+49515977	None:intergenic
AAAGAACTTCATCAATGAAT+TGG	0.387908	3_3:-49515317	Msa0456120:CDS
AAATACCAAATAAGTTTACT+AGG	0.488511	3_3:-49516357	Msa0456120:CDS
AAATATGTGTACATCAAACT+TGG	0.487598	3_3:+49516156	None:intergenic
AAATGGTTTCTATCTATCGG+CGG	0.576380	3_3:-49514964	Msa0456120:CDS
AATAAGTTTACTAGGAGACA+TGG	0.640204	3_3:-49516349	Msa0456120:CDS
AATCTATTTCCACTACATTC+CGG	0.489114	3_3:+49516131	None:intergenic
AATGGTTTCTATCTATCGGC+GGG	0.522564	3_3:-49514963	Msa0456120:CDS
ACTTCAAACAGATACATTAA+CGG	0.476013	3_3:+49514830	None:intergenic
ACTTTCCATTTAGTGCCATC+AGG	0.440549	3_3:+49516292	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTCTCCTAGTAAACTTATT+TGG	+	chr3_3:49514842-49514861	None:intergenic	30.0%
AATAAGTTTACTAGGAGACA+TGG	-	chr3_3:49514842-49514861	Msa0456120:CDS	30.0%
GCACTAAATGGAAAGTTTAT+TGG	-	chr3_3:49514906-49514925	Msa0456120:CDS	30.0%
TTGATGTACACATATTTGAC+CGG	-	chr3_3:49515041-49515060	Msa0456120:CDS	30.0%
AATCTATTTCCACTACATTC+CGG	+	chr3_3:49515063-49515082	None:intergenic	30.0%
TCTTAGATGAATGGCTTAAT+CGG	-	chr3_3:49515152-49515171	Msa0456120:intron	30.0%
CAAAGACTCAACCAATAATT+TGG	-	chr3_3:49515439-49515458	Msa0456120:intron	30.0%
ACATCAATTACCATCAAAGT+AGG	+	chr3_3:49515573-49515592	None:intergenic	30.0%
AGATGAAACTGCTTGTAATT+TGG	-	chr3_3:49515600-49515619	Msa0456120:intron	30.0%
TATACATAGAATTGCAGGAA+TGG	+	chr3_3:49516031-49516050	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	49514833	49516380	49514833	ID=Msa0456120;Name=Msa0456120
chr3_3	mRNA	49514833	49516380	49514833	ID=Msa0456120-mRNA-1;Parent=Msa0456120;Name=Msa0456120-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0\|0\|1\|1\|1\|3\|0\|269
chr3_3	exon	49515981	49516380	49515981	ID=Msa0456120-mRNA-1:exon:9385;Parent=Msa0456120-mRNA-1
chr3_3	exon	49515215	49515360	49515215	ID=Msa0456120-mRNA-1:exon:9384;Parent=Msa0456120-mRNA-1
chr3_3	exon	49514833	49515096	49514833	ID=Msa0456120-mRNA-1:exon:9383;Parent=Msa0456120-mRNA-1
chr3_3	CDS	49515981	49516380	49515981	ID=Msa0456120-mRNA-1:cds;Parent=Msa0456120-mRNA-1
chr3_3	CDS	49515215	49515360	49515215	ID=Msa0456120-mRNA-1:cds;Parent=Msa0456120-mRNA-1
chr3_3	CDS	49514833	49515096	49514833	ID=Msa0456120-mRNA-1:cds;Parent=Msa0456120-mRNA-1

Gene Sequence

>Msa0456120

CAAATACCAAATAAGTTTACTAGGAGACATGGAGTTCATCTATCAAATCCAGTGTTGATCAAGACTCCTGATGGCACTAAATGGAAAGTTTATTGGAAAAAAATCAATGGTGAGATTTGGTTTGAAAAGGGTTGGAAAACTTTTACTGAAAATTACTCTCTTGGACATGGCTGTTTGGTGGTGTTTGAATATAAAGGAACTTCCAAGTTTGATGTACACATATTTGACCGGAATGTAGTGGAAATAGATTATGATTCTTCATGTGACACTGATGATGAAAATGACAATGTTGATCATAGTGATGATGAATCACTTGAGATCTTAGATGAATGGCTTAATCGGAAGAAGGCTAGACAAAGGTCACCATTAGCTTCTCCTCGACCACATAAGAAAGTTAAAGGTGATCATGAGAAGACTACTAAAAGAACTTCATCAATGAATTGGCCAAAAGATGCTAGAGCTCAGGAAGTTGCTCAAAATTTCATCTCAACCAATCCTTTTTTCACAATTCTTGTAAAACCTCACCATACAGTAGATAGTAAGCTGTATGTAACTGCAGATTTAAAAGGAGTTATTGAGAACAAAGAGGAGTATGTGATTCTGCAGATTGGGAAAAGATCATGGAATGTGAAATTGATCTGTTCTAACGAGGCTAAAAATGGTTTCTATCTATCGGCGGGTTGGTCCTTGTTTGCAAGCGAAAGTGAACTGCAACCAGGAGATGTTTGTGTCTTTGAACTGATAAAAAAGAAAGATTTCGTTTTTAAAGTTCATGTCTTCAAAAAAGGGACCGTTAATGTATCTGTTTGA

Protein sequence

>Msa0456120

QIPNKFTRRHGVHLSNPVLIKTPDGTKWKVYWKKINGEIWFEKGWKTFTENYSLGHGCLVVFEYKGTSKFDVHIFDRNVVEIDYDSSCDTDDENDNVDHSDDESLEILDEWLNRKKARQRSPLASPRPHKKVKGDHEKTTKRTSSMNWPKDARAQEVAQNFISTNPFFTILVKPHHTVDSKLYVTADLKGVIENKEEYVILQIGKRSWNVKLICSNEAKNGFYLSAGWSLFASESELQPGDVCVFELIKKKDFVFKVHVFKKGTVNVSV*