AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0375240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0375240 MtrunA17_Chr3g0140331 92.331 326 14 4 1 323 47 364 0.0 544
Msa0375240 MtrunA17_Chr1g0146231 52.857 350 120 10 6 323 8 344 6.14e-107 315
Msa0375240 MtrunA17_Chr8g0340401 45.082 366 134 14 1 323 1 342 3.33e-80 247
Msa0375240 MtrunA17_Chr4g0035891 36.824 296 144 11 1 281 13 280 1.92e-43 151
Msa0375240 MtrunA17_Chr8g0372291 39.362 282 128 11 19 282 32 288 3.78e-42 147
Msa0375240 MtrunA17_Chr3g0145121 39.852 271 142 10 19 282 29 285 6.44e-40 142
Msa0375240 MtrunA17_Chr1g0199671 37.597 258 97 8 83 276 49 306 1.61e-37 136
Msa0375240 MtrunA17_Chr6g0461591 37.778 225 89 6 104 278 35 258 3.10e-36 131
Msa0375240 MtrunA17_Chr5g0404981 40.708 226 96 7 90 282 150 370 5.03e-35 131
Msa0375240 MtrunA17_Chr7g0253101 41.038 212 82 7 104 276 45 252 6.61e-35 127
Msa0375240 MtrunA17_Chr1g0169431 78.873 71 15 0 211 281 171 241 1.37e-33 124
Msa0375240 MtrunA17_Chr5g0395391 53.211 109 51 0 173 281 231 339 5.19e-33 124
Msa0375240 MtrunA17_Chr5g0395391 49.206 126 49 2 151 275 120 231 5.18e-30 116
Msa0375240 MtrunA17_Chr1g0213161 60.674 89 28 2 209 294 149 233 6.50e-30 114
Msa0375240 MtrunA17_Chr4g0028961 60.465 86 34 0 212 297 232 317 7.30e-30 115
Msa0375240 MtrunA17_Chr2g0326771 75.385 65 16 0 212 276 216 280 2.65e-29 114
Msa0375240 MtrunA17_Chr7g0232671 55.696 79 32 2 201 278 175 251 1.82e-22 94.4
Msa0375240 MtrunA17_Chr7g0232681 34.783 184 102 6 91 272 25 192 2.62e-21 89.7
Msa0375240 MtrunA17_Chr7g0232701 53.947 76 31 1 197 272 62 133 1.67e-20 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0375240 AT4G32890.1 49.684 316 114 10 30 323 16 308 5.29e-87 264
Msa0375240 AT5G25830.1 47.175 354 129 11 2 323 4 331 1.63e-85 261
Msa0375240 AT3G60530.1 48.039 204 74 5 89 292 63 234 1.71e-51 171
Msa0375240 AT2G45050.1 47.907 215 76 7 89 289 65 257 1.22e-48 164
Msa0375240 AT4G36240.1 39.300 257 111 8 32 279 11 231 3.12e-38 136
Msa0375240 AT5G66320.2 39.768 259 98 7 76 292 87 329 6.48e-38 138
Msa0375240 AT5G66320.1 39.768 259 98 7 76 292 87 329 6.48e-38 138
Msa0375240 AT3G24050.1 77.941 68 15 0 211 278 193 260 4.57e-33 123
Msa0375240 AT1G08010.2 67.073 82 21 1 209 284 217 298 4.65e-32 121
Msa0375240 AT1G08010.1 67.073 82 21 1 209 284 217 298 4.65e-32 121
Msa0375240 AT1G08010.3 67.073 82 21 1 209 284 217 298 4.65e-32 121
Msa0375240 AT4G34680.1 43.478 184 73 7 99 281 96 249 8.52e-32 120
Msa0375240 AT4G34680.2 43.478 184 73 7 99 281 96 249 8.52e-32 120
Msa0375240 AT3G54810.1 75.000 68 17 0 212 279 229 296 9.79e-32 121
Msa0375240 AT3G54810.2 75.000 68 17 0 212 279 229 296 9.79e-32 121
Msa0375240 AT3G51080.1 73.913 69 18 0 212 280 221 289 4.13e-30 116
Msa0375240 AT1G08000.2 60.227 88 30 1 209 291 215 302 4.32e-30 116
Msa0375240 AT1G08000.1 60.227 88 30 1 209 291 215 302 4.32e-30 116
Msa0375240 AT3G45170.1 62.319 69 26 0 212 280 115 183 5.61e-26 102
Msa0375240 AT2G28340.1 68.254 63 20 0 214 276 193 255 1.23e-24 101

Find 105 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGATTCCTCAGCAAATT+TGG 0.139979 3_1:+74886231 None:intergenic
CTACATGAGGTGCGTGAAAT+CGG 0.267854 3_1:+74885604 None:intergenic
CCATGGGTTAGGAGGTTGTT+AGG 0.285431 3_1:+74888083 None:intergenic
CACGCATTGCATAGTGTCTT+TGG 0.290696 3_1:+74885860 None:intergenic
ACAGTTGATATCTGGATTAA+AGG 0.306992 3_1:-74886185 Msa0375240:CDS
CATAAGCTACAGTTGATATC+TGG 0.309428 3_1:-74886193 Msa0375240:CDS
AAGGGCCGTCGGCAGAATTC+TGG 0.309787 3_1:+74887822 None:intergenic
CAGTGGCGCACCGGGCCATT+AGG 0.332794 3_1:-74885884 Msa0375240:CDS
CATAGTGTCTTTGGACCTAA+TGG 0.339675 3_1:+74885869 None:intergenic
GTGAGAGAGAAAAGCATAAG+AGG 0.370517 3_1:+74888197 None:intergenic
CACGTGTGCCTCCATGCAAC+TGG 0.370780 3_1:-74886069 Msa0375240:CDS
GGGCCCTCATCATCTCCTTC+TGG 0.374473 3_1:+74885723 None:intergenic
ATTGTGGACAGAAGCTGGTT+TGG 0.397002 3_1:+74888044 None:intergenic
GGTTAGGAGGTTGTTAGGTT+TGG 0.397903 3_1:+74888088 None:intergenic
TTGCAACAATTGGTGTTGTT+GGG 0.408578 3_1:+74885703 None:intergenic
GTTGCAACAATTGGTGTTGT+TGG 0.409369 3_1:+74885702 None:intergenic
GACACTTGAGGGTGTATTGT+TGG 0.425894 3_1:+74886121 None:intergenic
TGCTTAGTCAACACAAATGT+TGG 0.431309 3_1:+74885782 None:intergenic
CATCACAGCATTATGTTTGA+AGG 0.433831 3_1:-74885671 Msa0375240:CDS
ACAAAGTGTGACAAAGTTTG+TGG 0.433866 3_1:+74888165 None:intergenic
TCTCTTTGATTTCTCTAATG+AGG 0.434233 3_1:-74887957 Msa0375240:CDS
CGGCAAAAGCCCGTAGTAAA+CGG 0.443499 3_1:-74886093 Msa0375240:CDS
TTCGAAAGTGGGGTCTTCAA+TGG 0.445894 3_1:+74887928 None:intergenic
TACATGAGGTGCGTGAAATC+GGG 0.448275 3_1:+74885605 None:intergenic
TTGCACCTTTCGATGTGAGT+TGG 0.456005 3_1:+74885757 None:intergenic
CTCCGGCGAACTCTCCGTTC+CGG 0.462198 3_1:-74887798 Msa0375240:intron
ACGGAGAGTTCGCCGGAGAA+AGG 0.464710 3_1:+74887803 None:intergenic
AGATGTGGTGGTGGTGGTCA+TGG 0.465812 3_1:+74886018 None:intergenic
GTTTGTGGTGGAATTGTTGT+CGG 0.468374 3_1:+74888021 None:intergenic
ACAAATGTTGGGCTTGCTGC+AGG 0.469450 3_1:+74885794 None:intergenic
GTGGTGGTGGTGGTCATGGT+TGG 0.470501 3_1:+74886022 None:intergenic
TTGCCGGGACAGACACTTGA+GGG 0.471708 3_1:+74886110 None:intergenic
ATGATCTTGCGCAGCTAGAA+TGG 0.476849 3_1:-74886258 Msa0375240:CDS
CCTCATGTAGTACGGTGCTT+TGG 0.478377 3_1:-74885591 Msa0375240:exon
GTGTGACAAAGTTTGTGGTT+TGG 0.486157 3_1:+74888170 None:intergenic
TCATCATCTCCTTCTGGCGT+CGG 0.486176 3_1:+74885729 None:intergenic
CGGAGAGTTCGCCGGAGAAA+GGG 0.487872 3_1:+74887804 None:intergenic
ATTAATATTCAAAGCTGCGT+TGG 0.490776 3_1:+74885553 None:intergenic
CTCTTGAGCTTCCATGGGTT+AGG 0.491426 3_1:+74888072 None:intergenic
AATATTCAAAGCTGCGTTGG+AGG 0.492539 3_1:+74885556 None:intergenic
GCTTAGTCAACACAAATGTT+GGG 0.498811 3_1:+74885783 None:intergenic
AGCAGTGTTTACATTGTTTG+TGG 0.499828 3_1:+74888006 None:intergenic
TCTGCCGACGGCCCTTTCTC+CGG 0.501462 3_1:-74887815 Msa0375240:CDS
GTGGGATTGGTTTCAAGAGG+AGG 0.506926 3_1:+74887875 None:intergenic
GTGGAATTGTTGTCGGATTG+TGG 0.509048 3_1:+74888028 None:intergenic
AGGAGGAGATTCTTCGAAAG+TGG 0.514544 3_1:+74887916 None:intergenic
GATGTTGTAGTTGCAACAAT+TGG 0.517809 3_1:+74885693 None:intergenic
TTGAGCTTCCATGGGTTAGG+AGG 0.518418 3_1:+74888075 None:intergenic
ACACTTGAGGGTGTATTGTT+GGG 0.518688 3_1:+74886122 None:intergenic
AGACTCCACAGTGGCGCACC+GGG 0.518894 3_1:-74885892 Msa0375240:CDS
AAGACTCCACAGTGGCGCAC+CGG 0.525177 3_1:-74885893 Msa0375240:CDS
GAATCATTAGAGTTGGTAGG+AGG 0.531077 3_1:+74887899 None:intergenic
GCAAGAGCTCCGACGCCAGA+AGG 0.533988 3_1:-74885738 Msa0375240:CDS
GTCGGATTGTGGACAGAAGC+TGG 0.535564 3_1:+74888039 None:intergenic
GGAGAATCATTAGAGTTGGT+AGG 0.541668 3_1:+74887896 None:intergenic
ATATCAACTGTAGCTTATGA+AGG 0.545098 3_1:+74886197 None:intergenic
AGGAGGAGAATCATTAGAGT+TGG 0.548174 3_1:+74887892 None:intergenic
ATAATGAAGTGAGAAAGTAT+AGG 0.561648 3_1:+74888128 None:intergenic
CACACGTGACCGTTTACTAC+GGG 0.563431 3_1:+74886084 None:intergenic
AATGGCCCGGTGCGCCACTG+TGG 0.564107 3_1:+74885887 None:intergenic
GGAGGAGATTCTTCGAAAGT+GGG 0.565310 3_1:+74887917 None:intergenic
TGTGGAGTCAGATACAAGTC+AGG 0.567755 3_1:-74885839 Msa0375240:CDS
TACCGGAACGGAGAGTTCGC+CGG 0.569597 3_1:+74887796 None:intergenic
GACGCCAGAAGGAGATGATG+AGG 0.570929 3_1:-74885727 Msa0375240:CDS
GAAGTGGGATTGGTTTCAAG+AGG 0.577002 3_1:+74887872 None:intergenic
AGAGATCTTCAACGATGAAG+TGG 0.579783 3_1:+74887975 None:intergenic
GTGAGAAGATGTGGTGGTGG+TGG 0.580310 3_1:+74886012 None:intergenic
GCACACGTGACCGTTTACTA+CGG 0.581455 3_1:+74886083 None:intergenic
AGGCGCGAAGTCCAGTTGCA+TGG 0.582876 3_1:+74886058 None:intergenic
TGTCTTTGGACCTAATGGCC+CGG 0.587867 3_1:+74885874 None:intergenic
GACCTCGGTGATGGAGGAGA+GGG 0.591558 3_1:-74885944 Msa0375240:CDS
TTTGCCGGGACAGACACTTG+AGG 0.592935 3_1:+74886109 None:intergenic
ACACCCTCAAGTGTCTGTCC+CGG 0.593472 3_1:-74886113 Msa0375240:CDS
AAAAGAGACCTCGGTGATGG+AGG 0.595010 3_1:-74885950 Msa0375240:CDS
AATCAAGTAATCCTCACCGT+TGG 0.595692 3_1:+74885643 None:intergenic
AGAGTGAGAAGATGTGGTGG+TGG 0.596239 3_1:+74886009 None:intergenic
AAGTAATCCTCACCGTTGGA+TGG 0.600786 3_1:+74885647 None:intergenic
TATAATGGCACGTACCGGAA+CGG 0.606350 3_1:+74887784 None:intergenic
ACGCCAGAAGGAGATGATGA+GGG 0.609091 3_1:-74885726 Msa0375240:CDS
AGACCTCGGTGATGGAGGAG+AGG 0.609530 3_1:-74885945 Msa0375240:CDS
CTTGCTGCAGGTCTATACTC+AGG 0.609954 3_1:+74885806 None:intergenic
TTCGCCGGAGAAAGGGCCGT+CGG 0.611125 3_1:+74887811 None:intergenic
GCACTCCAACTCACATCGAA+AGG 0.612333 3_1:-74885762 Msa0375240:CDS
TTCACGCACCTCATGTAGTA+CGG 0.615224 3_1:-74885599 Msa0375240:exon
CCTAACAACCTCCTAACCCA+TGG 0.616294 3_1:-74888083 Msa0375240:exon
AGTGTTTACATTGTTTGTGG+TGG 0.618078 3_1:+74888009 None:intergenic
ATCTTCAACGATGAAGTGGT+CGG 0.624703 3_1:+74887979 None:intergenic
ATCTTCTCACTCTGACACCA+TGG 0.629511 3_1:-74885999 Msa0375240:CDS
TCGTGCCAGAATTCTGCCGA+CGG 0.642132 3_1:-74887827 Msa0375240:CDS
GTCAGAGTGAGAAGATGTGG+TGG 0.644152 3_1:+74886006 None:intergenic
ATGTTTGAAGGACCATCCAA+CGG 0.648434 3_1:-74885659 Msa0375240:CDS
CCAAAGCACCGTACTACATG+AGG 0.651273 3_1:+74885591 None:intergenic
CCACTGTGGAGTCTTATCAG+TGG 0.652363 3_1:+74885901 None:intergenic
ATGGATGTCCAAATTTGCTG+AGG 0.653086 3_1:-74886239 Msa0375240:CDS
TGGTTGGAGAGAGAACTAGA+AGG 0.657111 3_1:+74886038 None:intergenic
CGCGAAGTCCAGTTGCATGG+AGG 0.662375 3_1:+74886061 None:intergenic
TACTTGATTCATCAACACGT+GGG 0.670141 3_1:-74885629 Msa0375240:CDS
GGTGTCAGAGTGAGAAGATG+TGG 0.671117 3_1:+74886003 None:intergenic
TGAAGGACCATCCAACGGTG+AGG 0.675304 3_1:-74885654 Msa0375240:CDS
AAGACACTATGCAATGCGTG+TGG 0.676419 3_1:-74885857 Msa0375240:CDS
GAGGAGATTCTTCGAAAGTG+GGG 0.692052 3_1:+74887918 None:intergenic
TTACTTGATTCATCAACACG+TGG 0.700709 3_1:-74885630 Msa0375240:CDS
GACCCTCTCCTCCATCACCG+AGG 0.717630 3_1:+74885942 None:intergenic
ATTCTGGCACGAATTGACGT+CGG 0.752925 3_1:+74887838 None:intergenic
CCACTGATAAGACTCCACAG+TGG 0.837860 3_1:-74885901 Msa0375240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTTATATACAACAAT+TGG - chr3_1:74885926-74885945 Msa0375240:CDS 10.0%
!! TATAGTTAAAAAAAATATAG+GGG + chr3_1:74886787-74886806 None:intergenic 10.0%
!! CTATAGTTAAAAAAAATATA+GGG + chr3_1:74886788-74886807 None:intergenic 10.0%
!! ACTATAGTTAAAAAAAATAT+AGG + chr3_1:74886789-74886808 None:intergenic 10.0%
!!! AAATAAAGAGAGTATTATTT+AGG + chr3_1:74886003-74886022 None:intergenic 15.0%
!! ATGTGACAAAATTAAAAATA+GGG + chr3_1:74886399-74886418 None:intergenic 15.0%
!! TATGTGACAAAATTAAAAAT+AGG + chr3_1:74886400-74886419 None:intergenic 15.0%
!! ATAGTTAAAAAAAATATAGG+GGG + chr3_1:74886786-74886805 None:intergenic 15.0%
!! ATATTTGTAATAAAAGCTAA+GGG + chr3_1:74886834-74886853 None:intergenic 15.0%
!! TATATTTGTAATAAAAGCTA+AGG + chr3_1:74886835-74886854 None:intergenic 15.0%
!!! TATTATTTTTGTTTAGAGAA+GGG + chr3_1:74886873-74886892 None:intergenic 15.0%
!!! TTATTATTTTTGTTTAGAGA+AGG + chr3_1:74886874-74886893 None:intergenic 15.0%
!!! TCTTAAGATATACTTTTTTT+AGG + chr3_1:74887024-74887043 None:intergenic 15.0%
!!! TCATTTTTAAGATACTATAT+CGG + chr3_1:74887064-74887083 None:intergenic 15.0%
!! ATATAAAGTAAGAATTCTAA+TGG - chr3_1:74887091-74887110 Msa0375240:intron 15.0%
!! TTTCATAAGTATGACAATTA+TGG + chr3_1:74886192-74886211 None:intergenic 20.0%
!! CAAAAGTAAAAAAATTGTCT+TGG + chr3_1:74886353-74886372 None:intergenic 20.0%
!!! CAAGACAATTTTTTTACTTT+TGG - chr3_1:74886351-74886370 Msa0375240:intron 20.0%
!! TCTTAAGACAACAATTAAAA+AGG - chr3_1:74887036-74887055 Msa0375240:intron 20.0%
!!! ATTAGGTTATGTATTTTCTA+AGG + chr3_1:74887242-74887261 None:intergenic 20.0%
!! AGAAAATACATAACCTAATA+TGG - chr3_1:74887243-74887262 Msa0375240:intron 20.0%
!!! TTCTTTTTATACTCCATATT+AGG + chr3_1:74887259-74887278 None:intergenic 20.0%
!! ATAATGAAGTGAGAAAGTAT+AGG + chr3_1:74885489-74885508 None:intergenic 25.0%
! AATGGAACAGATAAAATTGT+AGG + chr3_1:74885966-74885985 None:intergenic 25.0%
!!! TATAGTCTTTTATTTAGTCG+TGG - chr3_1:74886469-74886488 Msa0375240:intron 25.0%
!!! ATAGTCTTTTATTTAGTCGT+GGG - chr3_1:74886470-74886489 Msa0375240:intron 25.0%
! ATAAGAAAATGTGAGGTATA+TGG - chr3_1:74886495-74886514 Msa0375240:intron 25.0%
! TAAGAAAATGTGAGGTATAT+GGG - chr3_1:74886496-74886515 Msa0375240:intron 25.0%
!! GAGTGAATTTTGAATTTCAA+GGG - chr3_1:74886526-74886545 Msa0375240:intron 25.0%
!!! GTTTTTGATTCAACGTTATT+TGG + chr3_1:74887457-74887476 None:intergenic 25.0%
! TCTCTTTGATTTCTCTAATG+AGG - chr3_1:74885657-74885676 Msa0375240:CDS 30.0%
ATATATATAATGGCACGTAC+CGG + chr3_1:74885838-74885857 None:intergenic 30.0%
TATCTGTTCCATTGAATTTG+AGG - chr3_1:74885973-74885992 Msa0375240:CDS 30.0%
ATCTGTTCCATTGAATTTGA+GGG - chr3_1:74885974-74885993 Msa0375240:CDS 30.0%
CACACATATTAAAGAAAAGC+AGG + chr3_1:74886114-74886133 None:intergenic 30.0%
! GGAGTGAATTTTGAATTTCA+AGG - chr3_1:74886525-74886544 Msa0375240:intron 30.0%
AAATGATTCCTCAGCAAATT+TGG + chr3_1:74887386-74887405 None:intergenic 30.0%
ATATCAACTGTAGCTTATGA+AGG + chr3_1:74887420-74887439 None:intergenic 30.0%
! ACAGTTGATATCTGGATTAA+AGG - chr3_1:74887429-74887448 Msa0375240:intron 30.0%
ATTAATATTCAAAGCTGCGT+TGG + chr3_1:74888064-74888083 None:intergenic 30.0%
CGTCATAAACTAAAAATGGT+AGG + chr3_1:74888120-74888139 None:intergenic 30.0%
CTGTCGTCATAAACTAAAAA+TGG + chr3_1:74888124-74888143 None:intergenic 30.0%
!!! CATTTTTAGTTTATGACGAC+AGG - chr3_1:74888122-74888141 Msa0375240:five_prime_UTR 30.0%
ACAAAGTGTGACAAAGTTTG+TGG + chr3_1:74885452-74885471 None:intergenic 35.0%
AAAAACTCTTGAGCTTCCAT+GGG + chr3_1:74885550-74885569 None:intergenic 35.0%
AGTGTTTACATTGTTTGTGG+TGG + chr3_1:74885608-74885627 None:intergenic 35.0%
AGCAGTGTTTACATTGTTTG+TGG + chr3_1:74885611-74885630 None:intergenic 35.0%
GAAAAAATTGGAAGTGGGAT+TGG + chr3_1:74885755-74885774 None:intergenic 35.0%
TCGGTGAAAAAATTGGAAGT+GGG + chr3_1:74885760-74885779 None:intergenic 35.0%
ATTGACGTCGGTGAAAAAAT+TGG + chr3_1:74885767-74885786 None:intergenic 35.0%
TTGACTCAACATGTGTAGTT+AGG - chr3_1:74885892-74885911 Msa0375240:CDS 35.0%
TAGGTTTCCCTCAAATTCAA+TGG + chr3_1:74885984-74886003 None:intergenic 35.0%
ATTGGTTAAGCAGCTAAAAG+AGG - chr3_1:74886268-74886287 Msa0375240:CDS 35.0%
AAAATTAAAAATAGGGCCCC+GGG + chr3_1:74886392-74886411 None:intergenic 35.0%
CAAAATTAAAAATAGGGCCC+CGG + chr3_1:74886393-74886412 None:intergenic 35.0%
GTGGGAAATAAGAAAATGTG+AGG - chr3_1:74886488-74886507 Msa0375240:intron 35.0%
!!! TGAGGAATCATTTTCAAGTG+AGG - chr3_1:74887393-74887412 Msa0375240:intron 35.0%
! CATAAGCTACAGTTGATATC+TGG - chr3_1:74887421-74887440 Msa0375240:intron 35.0%
GCTTAGTCAACACAAATGTT+GGG + chr3_1:74887834-74887853 None:intergenic 35.0%
TGCTTAGTCAACACAAATGT+TGG + chr3_1:74887835-74887854 None:intergenic 35.0%
!! TTGCAACAATTGGTGTTGTT+GGG + chr3_1:74887914-74887933 None:intergenic 35.0%
GATGTTGTAGTTGCAACAAT+TGG + chr3_1:74887924-74887943 None:intergenic 35.0%
CATCACAGCATTATGTTTGA+AGG - chr3_1:74887943-74887962 Msa0375240:CDS 35.0%
TTACTTGATTCATCAACACG+TGG - chr3_1:74887984-74888003 Msa0375240:CDS 35.0%
TACTTGATTCATCAACACGT+GGG - chr3_1:74887985-74888004 Msa0375240:CDS 35.0%
GTGTGACAAAGTTTGTGGTT+TGG + chr3_1:74885447-74885466 None:intergenic 40.0%
GAAAAACTCTTGAGCTTCCA+TGG + chr3_1:74885551-74885570 None:intergenic 40.0%
! GTTTGTGGTGGAATTGTTGT+CGG + chr3_1:74885596-74885615 None:intergenic 40.0%
ATCTTCAACGATGAAGTGGT+CGG + chr3_1:74885638-74885657 None:intergenic 40.0%
AGAGATCTTCAACGATGAAG+TGG + chr3_1:74885642-74885661 None:intergenic 40.0%
!! GAATCATTAGAGTTGGTAGG+AGG + chr3_1:74885718-74885737 None:intergenic 40.0%
! GGAGAATCATTAGAGTTGGT+AGG + chr3_1:74885721-74885740 None:intergenic 40.0%
AGGAGGAGAATCATTAGAGT+TGG + chr3_1:74885725-74885744 None:intergenic 40.0%
GTCGGTGAAAAAATTGGAAG+TGG + chr3_1:74885761-74885780 None:intergenic 40.0%
AAATTAAAAATAGGGCCCCG+GGG + chr3_1:74886391-74886410 None:intergenic 40.0%
ATGTGAGGTATATGGGATAG+AGG - chr3_1:74886503-74886522 Msa0375240:intron 40.0%
TGTGAGGTATATGGGATAGA+GGG - chr3_1:74886504-74886523 Msa0375240:intron 40.0%
CCTTATGATACACACCTACA+CGG - chr3_1:74887184-74887203 Msa0375240:intron 40.0%
ATATCTTATGTTCGCCGTGT+AGG + chr3_1:74887201-74887220 None:intergenic 40.0%
!! ATGGATGTCCAAATTTGCTG+AGG - chr3_1:74887375-74887394 Msa0375240:intron 40.0%
! ACACTTGAGGGTGTATTGTT+GGG + chr3_1:74887495-74887514 None:intergenic 40.0%
!!! TTTCTTGGAGAGGGCTTTTT+TGG + chr3_1:74887646-74887665 None:intergenic 40.0%
! AGGTCTCTTTTTCTTGGAGA+GGG + chr3_1:74887655-74887674 None:intergenic 40.0%
TCTCCAAGAAAAAGAGACCT+CGG - chr3_1:74887655-74887674 Msa0375240:intron 40.0%
CATAGTGTCTTTGGACCTAA+TGG + chr3_1:74887748-74887767 None:intergenic 40.0%
!! GTTGCAACAATTGGTGTTGT+TGG + chr3_1:74887915-74887934 None:intergenic 40.0%
ATGTTTGAAGGACCATCCAA+CGG - chr3_1:74887955-74887974 Msa0375240:CDS 40.0%
AATCAAGTAATCCTCACCGT+TGG + chr3_1:74887974-74887993 None:intergenic 40.0%
AATATTCAAAGCTGCGTTGG+AGG + chr3_1:74888061-74888080 None:intergenic 40.0%
GGTTAGGAGGTTGTTAGGTT+TGG + chr3_1:74885529-74885548 None:intergenic 45.0%
ATTGTGGACAGAAGCTGGTT+TGG + chr3_1:74885573-74885592 None:intergenic 45.0%
GTGGAATTGTTGTCGGATTG+TGG + chr3_1:74885589-74885608 None:intergenic 45.0%
TTCGAAAGTGGGGTCTTCAA+TGG + chr3_1:74885689-74885708 None:intergenic 45.0%
GAGGAGATTCTTCGAAAGTG+GGG + chr3_1:74885699-74885718 None:intergenic 45.0%
GGAGGAGATTCTTCGAAAGT+GGG + chr3_1:74885700-74885719 None:intergenic 45.0%
AGGAGGAGATTCTTCGAAAG+TGG + chr3_1:74885701-74885720 None:intergenic 45.0%
!! GAAGTGGGATTGGTTTCAAG+AGG + chr3_1:74885745-74885764 None:intergenic 45.0%
ATTCTGGCACGAATTGACGT+CGG + chr3_1:74885779-74885798 None:intergenic 45.0%
TATAATGGCACGTACCGGAA+CGG + chr3_1:74885833-74885852 None:intergenic 45.0%
TGGATTCTTAACAAGTGCCC+CGG - chr3_1:74886371-74886390 Msa0375240:intron 45.0%
CCGTGTAGGTGTGTATCATA+AGG + chr3_1:74887187-74887206 None:intergenic 45.0%
ATGATCTTGCGCAGCTAGAA+TGG - chr3_1:74887356-74887375 Msa0375240:intron 45.0%
! GACACTTGAGGGTGTATTGT+TGG + chr3_1:74887496-74887515 None:intergenic 45.0%
TGGTTGGAGAGAGAACTAGA+AGG + chr3_1:74887579-74887598 None:intergenic 45.0%
ATCTTCTCACTCTGACACCA+TGG - chr3_1:74887615-74887634 Msa0375240:intron 45.0%
! GAGGTCTCTTTTTCTTGGAG+AGG + chr3_1:74887656-74887675 None:intergenic 45.0%
! TCACCGAGGTCTCTTTTTCT+TGG + chr3_1:74887661-74887680 None:intergenic 45.0%
AGAAAAAGAGACCTCGGTGA+TGG - chr3_1:74887661-74887680 Msa0375240:intron 45.0%
CACGCATTGCATAGTGTCTT+TGG + chr3_1:74887757-74887776 None:intergenic 45.0%
AAGACACTATGCAATGCGTG+TGG - chr3_1:74887757-74887776 Msa0375240:intron 45.0%
TGTGGAGTCAGATACAAGTC+AGG - chr3_1:74887775-74887794 Msa0375240:intron 45.0%
TTGCACCTTTCGATGTGAGT+TGG + chr3_1:74887860-74887879 None:intergenic 45.0%
AAGTAATCCTCACCGTTGGA+TGG + chr3_1:74887970-74887989 None:intergenic 45.0%
TACATGAGGTGCGTGAAATC+GGG + chr3_1:74888012-74888031 None:intergenic 45.0%
CTACATGAGGTGCGTGAAAT+CGG + chr3_1:74888013-74888032 None:intergenic 45.0%
TTCACGCACCTCATGTAGTA+CGG - chr3_1:74888015-74888034 Msa0375240:CDS 45.0%
!! AATAATTACTATATATATAA+TGG + chr3_1:74885848-74885867 None:intergenic 5.0%
!!! TAAAATGAATATTATTTTTA+AGG - chr3_1:74886219-74886238 Msa0375240:CDS 5.0%
!! TATAAATATATGATAAAAAA+AGG + chr3_1:74886453-74886472 None:intergenic 5.0%
!!! ATAAATTTTATTATATTTGA+TGG + chr3_1:74886958-74886977 None:intergenic 5.0%
!! CCATGGGTTAGGAGGTTGTT+AGG + chr3_1:74885534-74885553 None:intergenic 50.0%
CCTAACAACCTCCTAACCCA+TGG - chr3_1:74885531-74885550 Msa0375240:three_prime_UTR 50.0%
!! TTGAGCTTCCATGGGTTAGG+AGG + chr3_1:74885542-74885561 None:intergenic 50.0%
CTCTTGAGCTTCCATGGGTT+AGG + chr3_1:74885545-74885564 None:intergenic 50.0%
!! GTGGGATTGGTTTCAAGAGG+AGG + chr3_1:74885742-74885761 None:intergenic 50.0%
AGAATGTTAACCAGTGCCCC+CGG - chr3_1:74886242-74886261 Msa0375240:CDS 50.0%
TAGCTGCTTAACCAATGCCC+CGG + chr3_1:74886264-74886283 None:intergenic 50.0%
GGATTCTTAACAAGTGCCCC+GGG - chr3_1:74886372-74886391 Msa0375240:intron 50.0%
GATTCTTAACAAGTGCCCCG+GGG - chr3_1:74886373-74886392 Msa0375240:intron 50.0%
! GTTTACTACGGGCTTTTGCC+GGG + chr3_1:74887522-74887541 None:intergenic 50.0%
! CGTTTACTACGGGCTTTTGC+CGG + chr3_1:74887523-74887542 None:intergenic 50.0%
CGGCAAAAGCCCGTAGTAAA+CGG - chr3_1:74887521-74887540 Msa0375240:intron 50.0%
CACACGTGACCGTTTACTAC+GGG + chr3_1:74887533-74887552 None:intergenic 50.0%
GCACACGTGACCGTTTACTA+CGG + chr3_1:74887534-74887553 None:intergenic 50.0%
AGAGTGAGAAGATGTGGTGG+TGG + chr3_1:74887608-74887627 None:intergenic 50.0%
GTCAGAGTGAGAAGATGTGG+TGG + chr3_1:74887611-74887630 None:intergenic 50.0%
GGTGTCAGAGTGAGAAGATG+TGG + chr3_1:74887614-74887633 None:intergenic 50.0%
!!! CTTGGAGAGGGCTTTTTTGG+TGG + chr3_1:74887643-74887662 None:intergenic 50.0%
! AAAAGAGACCTCGGTGATGG+AGG - chr3_1:74887664-74887683 Msa0375240:intron 50.0%
! CCACTGTGGAGTCTTATCAG+TGG + chr3_1:74887716-74887735 None:intergenic 50.0%
CCACTGATAAGACTCCACAG+TGG - chr3_1:74887713-74887732 Msa0375240:intron 50.0%
TGTCTTTGGACCTAATGGCC+CGG + chr3_1:74887743-74887762 None:intergenic 50.0%
CTTGCTGCAGGTCTATACTC+AGG + chr3_1:74887811-74887830 None:intergenic 50.0%
!! ACAAATGTTGGGCTTGCTGC+AGG + chr3_1:74887823-74887842 None:intergenic 50.0%
GCACTCCAACTCACATCGAA+AGG - chr3_1:74887852-74887871 Msa0375240:CDS 50.0%
TCATCATCTCCTTCTGGCGT+CGG + chr3_1:74887888-74887907 None:intergenic 50.0%
ACGCCAGAAGGAGATGATGA+GGG - chr3_1:74887888-74887907 Msa0375240:CDS 50.0%
!! CCAAAGCACCGTACTACATG+AGG + chr3_1:74888026-74888045 None:intergenic 50.0%
CCTCATGTAGTACGGTGCTT+TGG - chr3_1:74888023-74888042 Msa0375240:CDS 50.0%
GTCGGATTGTGGACAGAAGC+TGG + chr3_1:74885578-74885597 None:intergenic 55.0%
TCGTGCCAGAATTCTGCCGA+CGG - chr3_1:74885787-74885806 Msa0375240:CDS 55.0%
GAATGTTAACCAGTGCCCCC+GGG - chr3_1:74886243-74886262 Msa0375240:CDS 55.0%
AATGTTAACCAGTGCCCCCG+GGG - chr3_1:74886244-74886263 Msa0375240:CDS 55.0%
AGCTGCTTAACCAATGCCCC+GGG + chr3_1:74886263-74886282 None:intergenic 55.0%
ACACCCTCAAGTGTCTGTCC+CGG - chr3_1:74887501-74887520 Msa0375240:intron 55.0%
TTGCCGGGACAGACACTTGA+GGG + chr3_1:74887507-74887526 None:intergenic 55.0%
TTTGCCGGGACAGACACTTG+AGG + chr3_1:74887508-74887527 None:intergenic 55.0%
!! AGATGTGGTGGTGGTGGTCA+TGG + chr3_1:74887599-74887618 None:intergenic 55.0%
!! GTGAGAAGATGTGGTGGTGG+TGG + chr3_1:74887605-74887624 None:intergenic 55.0%
!!! GGGCTTTTTTGGTGGAGCCA+TGG + chr3_1:74887635-74887654 None:intergenic 55.0%
GACGCCAGAAGGAGATGATG+AGG - chr3_1:74887887-74887906 Msa0375240:CDS 55.0%
TGAAGGACCATCCAACGGTG+AGG - chr3_1:74887960-74887979 Msa0375240:CDS 55.0%
AAGGGCCGTCGGCAGAATTC+TGG + chr3_1:74885795-74885814 None:intergenic 60.0%
CGGAGAGTTCGCCGGAGAAA+GGG + chr3_1:74885813-74885832 None:intergenic 60.0%
ACGGAGAGTTCGCCGGAGAA+AGG + chr3_1:74885814-74885833 None:intergenic 60.0%
TACCGGAACGGAGAGTTCGC+CGG + chr3_1:74885821-74885840 None:intergenic 60.0%
CTGCTTAACCAATGCCCCGG+GGG + chr3_1:74886261-74886280 None:intergenic 60.0%
GCTGCTTAACCAATGCCCCG+GGG + chr3_1:74886262-74886281 None:intergenic 60.0%
CACGTGTGCCTCCATGCAAC+TGG - chr3_1:74887545-74887564 Msa0375240:intron 60.0%
CGCGAAGTCCAGTTGCATGG+AGG + chr3_1:74887556-74887575 None:intergenic 60.0%
AGGCGCGAAGTCCAGTTGCA+TGG + chr3_1:74887559-74887578 None:intergenic 60.0%
!! GTGGTGGTGGTGGTCATGGT+TGG + chr3_1:74887595-74887614 None:intergenic 60.0%
!! AGACCTCGGTGATGGAGGAG+AGG - chr3_1:74887669-74887688 Msa0375240:intron 60.0%
!! GACCTCGGTGATGGAGGAGA+GGG - chr3_1:74887670-74887689 Msa0375240:intron 60.0%
AAGACTCCACAGTGGCGCAC+CGG - chr3_1:74887721-74887740 Msa0375240:intron 60.0%
GGGCCCTCATCATCTCCTTC+TGG + chr3_1:74887894-74887913 None:intergenic 60.0%
TCTGCCGACGGCCCTTTCTC+CGG - chr3_1:74885799-74885818 Msa0375240:CDS 65.0%
TTCGCCGGAGAAAGGGCCGT+CGG + chr3_1:74885806-74885825 None:intergenic 65.0%
CTCCGGCGAACTCTCCGTTC+CGG - chr3_1:74885816-74885835 Msa0375240:CDS 65.0%
GACCCTCTCCTCCATCACCG+AGG + chr3_1:74887675-74887694 None:intergenic 65.0%
AGACTCCACAGTGGCGCACC+GGG - chr3_1:74887722-74887741 Msa0375240:intron 65.0%
GCAAGAGCTCCGACGCCAGA+AGG - chr3_1:74887876-74887895 Msa0375240:CDS 65.0%
AACCAGTGCCCCCGGGGCAT+TGG - chr3_1:74886250-74886269 Msa0375240:CDS 70.0%
! AACCAATGCCCCGGGGGCAC+TGG + chr3_1:74886255-74886274 None:intergenic 70.0%
AATGGCCCGGTGCGCCACTG+TGG + chr3_1:74887730-74887749 None:intergenic 70.0%
CAGTGGCGCACCGGGCCATT+AGG - chr3_1:74887730-74887749 Msa0375240:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 74885418 74888218 74885418 ID=Msa0375240;Name=Msa0375240
chr3_1 mRNA 74885418 74888218 74885418 ID=Msa0375240-mRNA-1;Parent=Msa0375240;Name=Msa0375240-mRNA-1;_AED=0.01;_eAED=0.01;_QI=132|1|1|1|1|1|2|186|323
chr3_1 exon 74885418 74886287 74885418 ID=Msa0375240-mRNA-1:exon:17469;Parent=Msa0375240-mRNA-1
chr3_1 exon 74887799 74888218 74887799 ID=Msa0375240-mRNA-1:exon:17468;Parent=Msa0375240-mRNA-1
chr3_1 five_prime_UTR 74888087 74888218 74888087 ID=Msa0375240-mRNA-1:five_prime_utr;Parent=Msa0375240-mRNA-1
chr3_1 CDS 74887799 74888086 74887799 ID=Msa0375240-mRNA-1:cds;Parent=Msa0375240-mRNA-1
chr3_1 CDS 74885604 74886287 74885604 ID=Msa0375240-mRNA-1:cds;Parent=Msa0375240-mRNA-1
chr3_1 three_prime_UTR 74885418 74885603 74885418 ID=Msa0375240-mRNA-1:three_prime_utr;Parent=Msa0375240-mRNA-1
Gene Sequence

>Msa0375240

ATGGAAGCTCAAGAGTTTTTCCAAACCAGCTTCTGTCCACAATCCGACAACAATTCCACCACAAACAATGTAAACACTGCTGCTTCCGACCACTTCATCGTTGAAGATCTCTTTGATTTCTCTAATGAGGATGTTGCCATTGAAGACCCCACTTTCGAAGAATCTCCTCCTACCAACTCTAATGATTCTCCTCCTCTTGAAACCAATCCCACTTCCAATTTTTTCACCGACGTCAATTCGTGCCAGAATTCTGCCGACGGCCCTTTCTCCGGCGAACTCTCCGTTCCGCAATATGATGATCTTGCGCAGCTAGAATGGATGTCCAAATTTGCTGAGGAATCATTTTCAAGTGAGGACCTTCATAAGCTACAGTTGATATCTGGATTAAAGGCACCAAATAACGTTGAATCAAAAACTCATGAAGAACGCAACCCAACAATACACCCTCAAGTGTCTGTCCCGGCAAAAGCCCGTAGTAAACGGTCACGTGTGCCTCCATGCAACTGGACTTCGCGCCTTCTAGTTCTCTCTCCAACCATGACCACCACCACCACATCTTCTCACTCTGACACCATGGCTCCACCAAAAAAGCCCTCTCCAAGAAAAAGAGACCTCGGTGATGGAGGAGAGGGTCGCAAGTGTCTTCATTGCGCCACTGATAAGACTCCACAGTGGCGCACCGGGCCATTAGGTCCAAAGACACTATGCAATGCGTGTGGAGTCAGATACAAGTCAGGTCGACTCGTACCTGAGTATAGACCTGCAGCAAGCCCAACATTTGTGTTGACTAAGCACTCCAACTCACATCGAAAGGTGCAAGAGCTCCGACGCCAGAAGGAGATGATGAGGGCCCAACAACACCAATTGTTGCAACTACAACATCATCACAGCATTATGTTTGAAGGACCATCCAACGGTGAGGATTACTTGATTCATCAACACGTGGGACCCGATTTCACGCACCTCATGTAG
Protein sequence

>Msa0375240

MEAQEFFQTSFCPQSDNNSTTNNVNTAASDHFIVEDLFDFSNEDVAIEDPTFEESPPTNSNDSPPLETNPTSNFFTDVNSCQNSADGPFSGELSVPQYDDLAQLEWMSKFAEESFSSEDLHKLQLISGLKAPNNVESKTHEERNPTIHPQVSVPAKARSKRSRVPPCNWTSRLLVLSPTMTTTTTSSHSDTMAPPKKPSPRKRDLGDGGEGRKCLHCATDKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVQELRRQKEMMRAQQHQLLQLQHHHSIMFEGPSNGEDYLIHQHVGPDFTHLM*