AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0376210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0376210 MtrunA17_Chr3g0126471 98.333 120 2 0 1 120 1 120 2.95e-86 249
Msa0376210 MtrunA17_Chr1g0158871 88.034 117 13 1 4 119 1 117 4.82e-72 213
Msa0376210 MtrunA17_Chr8g0347191 71.429 119 34 0 2 120 54 172 8.96e-59 181
Msa0376210 MtrunA17_Chr7g0243271 58.491 106 43 1 15 119 7 112 1.15e-38 128
Msa0376210 MtrunA17_Chr1g0151661 49.550 111 50 1 16 120 28 138 1.48e-36 123
Msa0376210 MtrunA17_Chr3g0114121 50.926 108 51 2 15 120 60 167 4.45e-34 117
Msa0376210 MtrunA17_Chr4g0005501 46.154 117 58 2 8 119 32 148 1.11e-31 111
Msa0376210 MtrunA17_Chr5g0437611 50.476 105 49 2 19 120 44 148 3.14e-31 109
Msa0376210 MtrunA17_Chr5g0411391 49.515 103 50 1 20 120 43 145 1.95e-30 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0376210 AT5G53160.1 75.214 117 25 1 8 120 2 118 3.42e-61 184
Msa0376210 AT5G53160.2 75.214 117 25 1 8 120 2 118 6.60e-61 185
Msa0376210 AT4G27920.1 68.644 118 32 2 4 120 1 114 3.70e-56 172
Msa0376210 AT1G01360.1 71.552 116 31 1 5 120 7 120 1.36e-55 171
Msa0376210 AT4G01026.1 65.323 124 37 2 1 120 1 122 1.22e-49 157
Msa0376210 AT2G26040.1 50.495 101 49 1 21 120 22 122 8.05e-35 119
Msa0376210 AT2G38310.1 54.545 99 43 1 23 119 41 139 1.50e-34 119
Msa0376210 AT5G05440.1 51.923 104 48 2 19 120 43 146 1.90e-33 115
Msa0376210 AT2G40330.1 48.544 103 51 1 19 119 46 148 5.15e-31 110
Msa0376210 AT5G45860.1 52.427 103 41 2 18 119 1 96 1.15e-29 105
Msa0376210 AT5G45870.1 55.556 90 39 1 31 119 7 96 4.48e-29 103
Msa0376210 AT4G17870.1 51.485 101 47 1 21 119 17 117 6.81e-29 103
Msa0376210 AT1G73000.1 45.714 105 51 1 21 119 37 141 7.27e-28 101
Msa0376210 AT4G18620.1 47.000 100 46 1 27 119 2 101 3.23e-27 99.0
Msa0376210 AT5G46790.2 50.000 102 49 1 21 120 44 145 1.60e-22 88.2
Msa0376210 AT5G46790.1 50.000 102 49 1 21 120 44 145 1.60e-22 88.2

Find 33 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTAGGTATGGTCTT+TGG 0.253746 3_1:+76255282 Msa0376210:intron
ATTCTAAGCATCAGGATTAT+TGG 0.270575 3_1:+76255466 Msa0376210:CDS
GGATTATTGGTGGTGATCAT+AGG 0.376992 3_1:+76255479 Msa0376210:CDS
AGAGAAGTTGATGTTAAGTC+AGG 0.400572 3_1:+76255388 Msa0376210:CDS
AGATTGATTACAAGAGGAAC+AGG 0.418165 3_1:-76254618 None:intergenic
TTGGTGGTGATCATAGGCTT+AGG 0.430017 3_1:+76255485 Msa0376210:CDS
GTGAGGGGAAACCTTGAGAT+TGG 0.441356 3_1:+76255358 Msa0376210:CDS
ATGAGCATATTCTAAGCATC+AGG 0.464677 3_1:+76255458 Msa0376210:CDS
TACAACTAGTACTGAAAGAT+TGG 0.468120 3_1:+76255420 Msa0376210:CDS
AATATAAACCATTCGTGAGC+AGG 0.468294 3_1:+76255329 Msa0376210:CDS
TGGTGGTGATCATAGGCTTA+GGG 0.497834 3_1:+76255486 Msa0376210:CDS
CTTTCAGTACTAGTTGTAGC+AGG 0.511083 3_1:-76255415 None:intergenic
AGGTATGGTCTTTGGTGAGG+AGG 0.517669 3_1:+76255290 Msa0376210:intron
CTCTTAAACTCCCAATCTCA+AGG 0.524215 3_1:-76255369 None:intergenic
TGAGGGGAAACCTTGAGATT+GGG 0.526415 3_1:+76255359 Msa0376210:CDS
CGTGAGCAGGTGTGTGGTGA+GGG 0.527012 3_1:+76255342 Msa0376210:CDS
GAATATGCTCATTGTCATCA+AGG 0.528687 3_1:-76255447 None:intergenic
GAAGAACACTGATTCTGTCC+AGG 0.537074 3_1:-76254570 None:intergenic
TCTAGGTATGGTCTTTGGTG+AGG 0.537536 3_1:+76255287 Msa0376210:intron
AGTGTTTAACACCACGGAGA+TGG 0.545954 3_1:+76254518 Msa0376210:CDS
ACGAATGGTTTATATTTCTG+CGG 0.550997 3_1:-76255322 None:intergenic
AACTAGTACTGAAAGATTGG+AGG 0.551568 3_1:+76255423 Msa0376210:CDS
AGACATCACAATCAACAGCC+TGG 0.570498 3_1:+76254552 Msa0376210:CDS
CTAAGCATCAGGATTATTGG+TGG 0.576552 3_1:+76255469 Msa0376210:CDS
ACCACACACCTGCTCACGAA+TGG 0.579553 3_1:-76255337 None:intergenic
GAGAAGTTGATGTTAAGTCA+GGG 0.583074 3_1:+76255389 Msa0376210:CDS
TCGTGAGCAGGTGTGTGGTG+AGG 0.600369 3_1:+76255341 Msa0376210:CDS
TCGGCGATGGAGAAAATGAA+CGG 0.670990 3_1:+76254480 None:intergenic
ACCATTCGTGAGCAGGTGTG+TGG 0.686863 3_1:+76255336 Msa0376210:CDS
CAACGGAGTGTTTAACACCA+CGG 0.688274 3_1:+76254512 Msa0376210:CDS
GTGAGCAGGTGTGTGGTGAG+GGG 0.713491 3_1:+76255343 Msa0376210:CDS
ATGAACGGAACTGAAAACAA+CGG 0.716623 3_1:+76254495 Msa0376210:CDS
TCTGATGTACTCCATCTCCG+TGG 0.739793 3_1:-76254529 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTAGCTTTT+CGG + chr3_1:76254696-76254715 Msa0376210:intron 10.0%
!!! TTTTTTTTTTTTTAGCTTTT+CGG + chr3_1:76254696-76254715 Msa0376210:intron 10.0%
!! TAATAGAATCATTTAAACTA+TGG - chr3_1:76254942-76254961 None:intergenic 15.0%
!!! TTTTTCAAAAATTTTACTTG+TGG + chr3_1:76255050-76255069 Msa0376210:intron 15.0%
!! TAATAGAATCATTTAAACTA+TGG - chr3_1:76254942-76254961 None:intergenic 15.0%
!!! TTTTTCAAAAATTTTACTTG+TGG + chr3_1:76255050-76255069 Msa0376210:intron 15.0%
! ATAGTAAGATTGATTACAAG+AGG - chr3_1:76254627-76254646 None:intergenic 25.0%
! AGAATCTAGCAAATCAAAAA+CGG - chr3_1:76254669-76254688 None:intergenic 25.0%
!!! GTTTTTTTTTTCCTTTCTCT+AGG + chr3_1:76255270-76255289 Msa0376210:intron 25.0%
! ATAGTAAGATTGATTACAAG+AGG - chr3_1:76254627-76254646 None:intergenic 25.0%
! AGAATCTAGCAAATCAAAAA+CGG - chr3_1:76254669-76254688 None:intergenic 25.0%
!!! GTTTTTTTTTTCCTTTCTCT+AGG + chr3_1:76255270-76255289 Msa0376210:intron 25.0%
TGATATTACGATGTAGAGTT+TGG + chr3_1:76255156-76255175 Msa0376210:intron 30.0%
GATTCTACTATGTTTCTCAA+TGG + chr3_1:76255230-76255249 Msa0376210:intron 30.0%
!!! TTTTTTCCTTTCTCTAGGTA+TGG + chr3_1:76255275-76255294 Msa0376210:intron 30.0%
! ACGAATGGTTTATATTTCTG+CGG - chr3_1:76255325-76255344 None:intergenic 30.0%
! TACAACTAGTACTGAAAGAT+TGG + chr3_1:76255420-76255439 Msa0376210:CDS 30.0%
ATTCTAAGCATCAGGATTAT+TGG + chr3_1:76255466-76255485 Msa0376210:CDS 30.0%
TGATATTACGATGTAGAGTT+TGG + chr3_1:76255156-76255175 Msa0376210:intron 30.0%
GATTCTACTATGTTTCTCAA+TGG + chr3_1:76255230-76255249 Msa0376210:intron 30.0%
!!! TTTTTTCCTTTCTCTAGGTA+TGG + chr3_1:76255275-76255294 Msa0376210:intron 30.0%
! ACGAATGGTTTATATTTCTG+CGG - chr3_1:76255325-76255344 None:intergenic 30.0%
! TACAACTAGTACTGAAAGAT+TGG + chr3_1:76255420-76255439 Msa0376210:CDS 30.0%
ATTCTAAGCATCAGGATTAT+TGG + chr3_1:76255466-76255485 Msa0376210:CDS 30.0%
ATGAACGGAACTGAAAACAA+CGG + chr3_1:76254495-76254514 Msa0376210:CDS 35.0%
AGATTGATTACAAGAGGAAC+AGG - chr3_1:76254621-76254640 None:intergenic 35.0%
AGCAATTCCAATGAGAAACA+TGG - chr3_1:76254898-76254917 None:intergenic 35.0%
AATATAAACCATTCGTGAGC+AGG + chr3_1:76255329-76255348 Msa0376210:CDS 35.0%
!! AGAGAAGTTGATGTTAAGTC+AGG + chr3_1:76255388-76255407 Msa0376210:CDS 35.0%
!! GAGAAGTTGATGTTAAGTCA+GGG + chr3_1:76255389-76255408 Msa0376210:CDS 35.0%
! AACTAGTACTGAAAGATTGG+AGG + chr3_1:76255423-76255442 Msa0376210:CDS 35.0%
GAATATGCTCATTGTCATCA+AGG - chr3_1:76255450-76255469 None:intergenic 35.0%
ATGAGCATATTCTAAGCATC+AGG + chr3_1:76255458-76255477 Msa0376210:CDS 35.0%
ATGAACGGAACTGAAAACAA+CGG + chr3_1:76254495-76254514 Msa0376210:CDS 35.0%
AGATTGATTACAAGAGGAAC+AGG - chr3_1:76254621-76254640 None:intergenic 35.0%
AGCAATTCCAATGAGAAACA+TGG - chr3_1:76254898-76254917 None:intergenic 35.0%
AATATAAACCATTCGTGAGC+AGG + chr3_1:76255329-76255348 Msa0376210:CDS 35.0%
!! AGAGAAGTTGATGTTAAGTC+AGG + chr3_1:76255388-76255407 Msa0376210:CDS 35.0%
!! GAGAAGTTGATGTTAAGTCA+GGG + chr3_1:76255389-76255408 Msa0376210:CDS 35.0%
! AACTAGTACTGAAAGATTGG+AGG + chr3_1:76255423-76255442 Msa0376210:CDS 35.0%
GAATATGCTCATTGTCATCA+AGG - chr3_1:76255450-76255469 None:intergenic 35.0%
ATGAGCATATTCTAAGCATC+AGG + chr3_1:76255458-76255477 Msa0376210:CDS 35.0%
!!! TCCAGCACTGACACTTTTAA+TGG + chr3_1:76254743-76254762 Msa0376210:intron 40.0%
!! TCCATTAAAAGTGTCAGTGC+TGG - chr3_1:76254747-76254766 None:intergenic 40.0%
!! GCACTGACACTTTTAATGGA+AGG + chr3_1:76254747-76254766 Msa0376210:intron 40.0%
GATTCTGCCATGTTTCTCAT+TGG + chr3_1:76254888-76254907 Msa0376210:intron 40.0%
! ACGCATTTCATCAGAAGTGT+CGG - chr3_1:76254965-76254984 None:intergenic 40.0%
ATGTAATTCGCATGTGTCAG+TGG - chr3_1:76255017-76255036 None:intergenic 40.0%
CAAAGACCATACCTAGAGAA+AGG - chr3_1:76255284-76255303 None:intergenic 40.0%
CTTTCTCTAGGTATGGTCTT+TGG + chr3_1:76255282-76255301 Msa0376210:intron 40.0%
CTCTTAAACTCCCAATCTCA+AGG - chr3_1:76255372-76255391 None:intergenic 40.0%
CTTTCAGTACTAGTTGTAGC+AGG - chr3_1:76255418-76255437 None:intergenic 40.0%
CTAAGCATCAGGATTATTGG+TGG + chr3_1:76255469-76255488 Msa0376210:CDS 40.0%
!! GGATTATTGGTGGTGATCAT+AGG + chr3_1:76255479-76255498 Msa0376210:CDS 40.0%
!!! TCCAGCACTGACACTTTTAA+TGG + chr3_1:76254743-76254762 Msa0376210:intron 40.0%
!! TCCATTAAAAGTGTCAGTGC+TGG - chr3_1:76254747-76254766 None:intergenic 40.0%
!! GCACTGACACTTTTAATGGA+AGG + chr3_1:76254747-76254766 Msa0376210:intron 40.0%
GATTCTGCCATGTTTCTCAT+TGG + chr3_1:76254888-76254907 Msa0376210:intron 40.0%
! ACGCATTTCATCAGAAGTGT+CGG - chr3_1:76254965-76254984 None:intergenic 40.0%
ATGTAATTCGCATGTGTCAG+TGG - chr3_1:76255017-76255036 None:intergenic 40.0%
CAAAGACCATACCTAGAGAA+AGG - chr3_1:76255284-76255303 None:intergenic 40.0%
CTTTCTCTAGGTATGGTCTT+TGG + chr3_1:76255282-76255301 Msa0376210:intron 40.0%
CTCTTAAACTCCCAATCTCA+AGG - chr3_1:76255372-76255391 None:intergenic 40.0%
CTTTCAGTACTAGTTGTAGC+AGG - chr3_1:76255418-76255437 None:intergenic 40.0%
CTAAGCATCAGGATTATTGG+TGG + chr3_1:76255469-76255488 Msa0376210:CDS 40.0%
!! GGATTATTGGTGGTGATCAT+AGG + chr3_1:76255479-76255498 Msa0376210:CDS 40.0%
! CAACGGAGTGTTTAACACCA+CGG + chr3_1:76254512-76254531 Msa0376210:CDS 45.0%
AGTGTTTAACACCACGGAGA+TGG + chr3_1:76254518-76254537 Msa0376210:CDS 45.0%
AGACATCACAATCAACAGCC+TGG + chr3_1:76254552-76254571 Msa0376210:CDS 45.0%
!! GAAGAACACTGATTCTGTCC+AGG - chr3_1:76254573-76254592 None:intergenic 45.0%
CTTCTGATGAAATGCGTGTC+TGG + chr3_1:76254968-76254987 Msa0376210:intron 45.0%
ATGAAGCACAGCACATACAC+TGG - chr3_1:76255096-76255115 None:intergenic 45.0%
TACGATGTAGAGTTTGGCTG+TGG + chr3_1:76255162-76255181 Msa0376210:intron 45.0%
TCTAGGTATGGTCTTTGGTG+AGG + chr3_1:76255287-76255306 Msa0376210:intron 45.0%
TGAGGGGAAACCTTGAGATT+GGG + chr3_1:76255359-76255378 Msa0376210:CDS 45.0%
!! TTGGTGGTGATCATAGGCTT+AGG + chr3_1:76255485-76255504 Msa0376210:CDS 45.0%
! CAACGGAGTGTTTAACACCA+CGG + chr3_1:76254512-76254531 Msa0376210:CDS 45.0%
AGTGTTTAACACCACGGAGA+TGG + chr3_1:76254518-76254537 Msa0376210:CDS 45.0%
AGACATCACAATCAACAGCC+TGG + chr3_1:76254552-76254571 Msa0376210:CDS 45.0%
!! GAAGAACACTGATTCTGTCC+AGG - chr3_1:76254573-76254592 None:intergenic 45.0%
CTTCTGATGAAATGCGTGTC+TGG + chr3_1:76254968-76254987 Msa0376210:intron 45.0%
ATGAAGCACAGCACATACAC+TGG - chr3_1:76255096-76255115 None:intergenic 45.0%
TACGATGTAGAGTTTGGCTG+TGG + chr3_1:76255162-76255181 Msa0376210:intron 45.0%
TCTAGGTATGGTCTTTGGTG+AGG + chr3_1:76255287-76255306 Msa0376210:intron 45.0%
TGAGGGGAAACCTTGAGATT+GGG + chr3_1:76255359-76255378 Msa0376210:CDS 45.0%
TCTGATGTACTCCATCTCCG+TGG - chr3_1:76254532-76254551 None:intergenic 50.0%
TGGAAGACACCAACACGTGT+CGG - chr3_1:76254997-76255016 None:intergenic 50.0%
AGGTATGGTCTTTGGTGAGG+AGG + chr3_1:76255290-76255309 Msa0376210:intron 50.0%
GTGAGGGGAAACCTTGAGAT+TGG + chr3_1:76255358-76255377 Msa0376210:CDS 50.0%
TCTGATGTACTCCATCTCCG+TGG - chr3_1:76254532-76254551 None:intergenic 50.0%
TGGAAGACACCAACACGTGT+CGG - chr3_1:76254997-76255016 None:intergenic 50.0%
AGGTATGGTCTTTGGTGAGG+AGG + chr3_1:76255290-76255309 Msa0376210:intron 50.0%
GTGAGGGGAAACCTTGAGAT+TGG + chr3_1:76255358-76255377 Msa0376210:CDS 50.0%
GTCTGGTATCCGACACGTGT+TGG + chr3_1:76254985-76255004 Msa0376210:intron 55.0%
ACCATTCGTGAGCAGGTGTG+TGG + chr3_1:76255336-76255355 Msa0376210:CDS 55.0%
ACCACACACCTGCTCACGAA+TGG - chr3_1:76255340-76255359 None:intergenic 55.0%
GTCTGGTATCCGACACGTGT+TGG + chr3_1:76254985-76255004 Msa0376210:intron 55.0%
ACCATTCGTGAGCAGGTGTG+TGG + chr3_1:76255336-76255355 Msa0376210:CDS 55.0%
ACCACACACCTGCTCACGAA+TGG - chr3_1:76255340-76255359 None:intergenic 55.0%
TCGTGAGCAGGTGTGTGGTG+AGG + chr3_1:76255341-76255360 Msa0376210:CDS 60.0%
CGTGAGCAGGTGTGTGGTGA+GGG + chr3_1:76255342-76255361 Msa0376210:CDS 60.0%
GTGAGCAGGTGTGTGGTGAG+GGG + chr3_1:76255343-76255362 Msa0376210:CDS 60.0%
TCGTGAGCAGGTGTGTGGTG+AGG + chr3_1:76255341-76255360 Msa0376210:CDS 60.0%
CGTGAGCAGGTGTGTGGTGA+GGG + chr3_1:76255342-76255361 Msa0376210:CDS 60.0%
GTGAGCAGGTGTGTGGTGAG+GGG + chr3_1:76255343-76255362 Msa0376210:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 76254486 76255507 76254486 ID=Msa0376210;Name=Msa0376210
chr3_1 mRNA 76254486 76255507 76254486 ID=Msa0376210-mRNA-1;Parent=Msa0376210;Name=Msa0376210-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|120
chr3_1 exon 76254486 76254629 76254486 ID=Msa0376210-mRNA-1:exon:17999;Parent=Msa0376210-mRNA-1
chr3_1 exon 76255292 76255507 76255292 ID=Msa0376210-mRNA-1:exon:18000;Parent=Msa0376210-mRNA-1
chr3_1 CDS 76254486 76254629 76254486 ID=Msa0376210-mRNA-1:cds;Parent=Msa0376210-mRNA-1
chr3_1 CDS 76255292 76255507 76255292 ID=Msa0376210-mRNA-1:cds;Parent=Msa0376210-mRNA-1
Gene Sequence

>Msa0376210

ATGGAGAAAATGAACGGAACTGAAAACAACGGAGTGTTTAACACCACGGAGATGGAGTACATCAGAAGACATCACAATCAACAGCCTGGACAGAATCAGTGTTCTTCTGCACTTGTTAAGCATATCAGAGCTCCTGTTCCTCTTGTATGGTCTTTGGTGAGGAGGTTTGATCAGCCGCAGAAATATAAACCATTCGTGAGCAGGTGTGTGGTGAGGGGAAACCTTGAGATTGGGAGTTTAAGAGAAGTTGATGTTAAGTCAGGGCTTCCTGCTACAACTAGTACTGAAAGATTGGAGGTCCTTGATGACAATGAGCATATTCTAAGCATCAGGATTATTGGTGGTGATCATAGGCTTAGG

Protein sequence

>Msa0376210

MEKMNGTENNGVFNTTEMEYIRRHHNQQPGQNQCSSALVKHIRAPVPLVWSLVRRFDQPQKYKPFVSRCVVRGNLEIGSLREVDVKSGLPATTSTERLEVLDDNEHILSIRIIGGDHRLR