AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0377070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0377070 MtrunA17_Chr3g0131921 98.718 78 1 0 1 78 1 78 9.53e-55 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0377070 AT5G50810.1 67.105 76 25 0 1 76 1 76 2.99e-30 102

Find 37 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTAATATTTAGCAAATTT+TGG 0.095082 3_1:-77450600 Msa0377070:three_prime_UTR
TTGTGCTGTGTTCGTTCATT+AGG 0.201592 3_1:-77451854 Msa0377070:five_prime_UTR
TGTGCTGTGTTCGTTCATTA+GGG 0.329856 3_1:-77451853 Msa0377070:five_prime_UTR
AAGAGCCATGATTAATGAAA+TGG 0.403854 3_1:-77450832 Msa0377070:CDS
TGCCACCATTTCATTAATCA+TGG 0.406262 3_1:+77450827 None:intergenic
AAATGCATCACAGGCACGCC+TGG 0.412006 3_1:-77450780 Msa0377070:CDS
GCGATTGAGAGGAGAGAGAG+AGG 0.454188 3_1:+77451802 None:intergenic
CCTGGGAATAAGTTCAGTTC+CGG 0.455891 3_1:-77450762 Msa0377070:CDS
AAAATAGCAATCAGAACCTT+CGG 0.481003 3_1:+77450291 None:intergenic
GATTGAGAGGAGAGAGAGAG+GGG 0.490740 3_1:+77451804 None:intergenic
CAAAGATGACTAGTCAATGC+TGG 0.492799 3_1:-77450806 Msa0377070:CDS
TCTCAATCGCTCTCTCTCAA+TGG 0.494922 3_1:-77451789 Msa0377070:exon
TCTGCATTTCAGCAGAATTG+AGG 0.501147 3_1:+77451756 None:intergenic
TTACTGCTTGTTTGACTGAA+GGG 0.501469 3_1:-77450484 Msa0377070:three_prime_UTR
CGATTGAGAGGAGAGAGAGA+GGG 0.507573 3_1:+77451803 None:intergenic
ACACTGTGCACAGCGTTACA+TGG 0.508169 3_1:-77450721 Msa0377070:CDS
AACGCTGTGCACAGTGTGTT+AGG 0.520352 3_1:+77450727 None:intergenic
CAAGAACATGGCAAGACCCT+TGG 0.523453 3_1:+77450372 None:intergenic
TGCTGGGACAAATGCATCAC+AGG 0.525844 3_1:-77450789 Msa0377070:CDS
TGAGCATGCTTATCATGAAA+CGG 0.531078 3_1:-77450695 Msa0377070:CDS
CTTACTGCTTGTTTGACTGA+AGG 0.539908 3_1:-77450485 Msa0377070:three_prime_UTR
ATTTCTTGTTCGTGTGCCGA+AGG 0.541962 3_1:-77450307 Msa0377070:three_prime_UTR
TTAATTAAGAAAGCACATCA+AGG 0.547393 3_1:+77450644 None:intergenic
CCGGAACTGAACTTATTCCC+AGG 0.566395 3_1:+77450762 None:intergenic
AAAGATGACTAGTCAATGCT+GGG 0.584821 3_1:-77450805 Msa0377070:CDS
GAAATTTGAAAGCAAGAACA+TGG 0.585640 3_1:+77450360 None:intergenic
AATGCATCACAGGCACGCCT+GGG 0.596603 3_1:-77450779 Msa0377070:CDS
TGTTAGGCAATTAGTCTCAC+CGG 0.596955 3_1:+77450743 None:intergenic
ATTGAGAGAGAGCGATTGAG+AGG 0.597246 3_1:+77451791 None:intergenic
TCAATTCTGCTGAAATGCAG+AGG 0.611780 3_1:-77451754 Msa0377070:CDS
AGCCATGATTAATGAAATGG+TGG 0.612893 3_1:-77450829 Msa0377070:CDS
GATATATTATCAGCTTACCA+AGG 0.625326 3_1:-77450389 Msa0377070:three_prime_UTR
CATTTCAGCAGAATTGAGGT+CGG 0.629707 3_1:+77451760 None:intergenic
GAGTATGCAATGAAGATCAA+CGG 0.630450 3_1:-77450667 Msa0377070:exon
ATTGAGAGGAGAGAGAGAGG+GGG 0.634879 3_1:+77451805 None:intergenic
ATATATTATCAGCTTACCAA+GGG 0.650187 3_1:-77450388 Msa0377070:three_prime_UTR
GATGAAAAGCTCAGTGCATG+TGG 0.677105 3_1:-77450515 Msa0377070:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGATATTTAAAAGTGATT+TGG - chr3_1:77450806-77450825 Msa0377070:CDS 15.0%
!!! ATTTTTTTCCATAAGATAAA+GGG + chr3_1:77450927-77450946 None:intergenic 15.0%
!!! TATTTTTTTCCATAAGATAA+AGG + chr3_1:77450928-77450947 None:intergenic 15.0%
!! AAGAACAAAAATTATTAATG+TGG - chr3_1:77451734-77451753 Msa0377070:intron 15.0%
!! AGAACAAAAATTATTAATGT+GGG - chr3_1:77451735-77451754 Msa0377070:intron 15.0%
!! TTTGATATTTAAAAGTGATT+TGG - chr3_1:77450806-77450825 Msa0377070:CDS 15.0%
!!! ATTTTTTTCCATAAGATAAA+GGG + chr3_1:77450927-77450946 None:intergenic 15.0%
!!! TATTTTTTTCCATAAGATAA+AGG + chr3_1:77450928-77450947 None:intergenic 15.0%
!! AAGAACAAAAATTATTAATG+TGG - chr3_1:77451734-77451753 Msa0377070:intron 15.0%
!! AGAACAAAAATTATTAATGT+GGG - chr3_1:77451735-77451754 Msa0377070:intron 15.0%
!!! TTTTTTTCCATAAGATAAAG+GGG + chr3_1:77450926-77450945 None:intergenic 20.0%
!! TCTAATAATAGTTGTAACTA+TGG - chr3_1:77450976-77450995 Msa0377070:intron 20.0%
!! ATTATCATTATTATGGCTTA+GGG - chr3_1:77451155-77451174 Msa0377070:intron 20.0%
!!! CGTTAATATTTAGCAAATTT+TGG - chr3_1:77451567-77451586 Msa0377070:intron 20.0%
!!! TTTTTTTCCATAAGATAAAG+GGG + chr3_1:77450926-77450945 None:intergenic 20.0%
!! TCTAATAATAGTTGTAACTA+TGG - chr3_1:77450976-77450995 Msa0377070:intron 20.0%
!! ATTATCATTATTATGGCTTA+GGG - chr3_1:77451155-77451174 Msa0377070:intron 20.0%
!!! CGTTAATATTTAGCAAATTT+TGG - chr3_1:77451567-77451586 Msa0377070:intron 20.0%
!!! AATGTAATCGAGTTGAAAAA+GGG + chr3_1:77450489-77450508 None:intergenic 25.0%
!!! AAATGTAATCGAGTTGAAAA+AGG + chr3_1:77450490-77450509 None:intergenic 25.0%
! AATAGTTGTAACTATGGAAT+TGG - chr3_1:77450982-77451001 Msa0377070:intron 25.0%
! CTAAGCCATAATAATGATAA+TGG + chr3_1:77451156-77451175 None:intergenic 25.0%
! CATTATCATTATTATGGCTT+AGG - chr3_1:77451154-77451173 Msa0377070:intron 25.0%
! TTATCATTATTATGGCTTAG+GGG - chr3_1:77451156-77451175 Msa0377070:intron 25.0%
! CACATATAAGTGGTATATAT+CGG + chr3_1:77451196-77451215 None:intergenic 25.0%
! AGATTGAAATACCATATAAG+TGG + chr3_1:77451236-77451255 None:intergenic 25.0%
! GTTGTTATATTTCATTACAG+GGG + chr3_1:77451280-77451299 None:intergenic 25.0%
! GGTTGTTATATTTCATTACA+GGG + chr3_1:77451281-77451300 None:intergenic 25.0%
!! TTAATTAAGAAAGCACATCA+AGG + chr3_1:77451526-77451545 None:intergenic 25.0%
! ATATATTATCAGCTTACCAA+GGG - chr3_1:77451779-77451798 Msa0377070:exon 25.0%
!!! AATGTAATCGAGTTGAAAAA+GGG + chr3_1:77450489-77450508 None:intergenic 25.0%
!!! AAATGTAATCGAGTTGAAAA+AGG + chr3_1:77450490-77450509 None:intergenic 25.0%
! AATAGTTGTAACTATGGAAT+TGG - chr3_1:77450982-77451001 Msa0377070:intron 25.0%
! CTAAGCCATAATAATGATAA+TGG + chr3_1:77451156-77451175 None:intergenic 25.0%
! CATTATCATTATTATGGCTT+AGG - chr3_1:77451154-77451173 Msa0377070:intron 25.0%
! TTATCATTATTATGGCTTAG+GGG - chr3_1:77451156-77451175 Msa0377070:intron 25.0%
! CACATATAAGTGGTATATAT+CGG + chr3_1:77451196-77451215 None:intergenic 25.0%
! AGATTGAAATACCATATAAG+TGG + chr3_1:77451236-77451255 None:intergenic 25.0%
! GTTGTTATATTTCATTACAG+GGG + chr3_1:77451280-77451299 None:intergenic 25.0%
! GGTTGTTATATTTCATTACA+GGG + chr3_1:77451281-77451300 None:intergenic 25.0%
!! TTAATTAAGAAAGCACATCA+AGG + chr3_1:77451526-77451545 None:intergenic 25.0%
! ATATATTATCAGCTTACCAA+GGG - chr3_1:77451779-77451798 Msa0377070:exon 25.0%
AGTATCTGTTAATCGTAAGA+AGG + chr3_1:77450466-77450485 None:intergenic 30.0%
TAACAGTTATCACTAAGACA+AGG + chr3_1:77450625-77450644 None:intergenic 30.0%
!! AGATTTGATTTTTCATGCTG+AGG - chr3_1:77450659-77450678 Msa0377070:exon 30.0%
! TATCAATTTTATGGTGGCTA+AGG - chr3_1:77450683-77450702 Msa0377070:CDS 30.0%
!! ATAGGTTTTTAAGAGAGACA+TGG + chr3_1:77451056-77451075 None:intergenic 30.0%
!! CATAATAATGATAATGGCAG+TGG + chr3_1:77451150-77451169 None:intergenic 30.0%
CACTGCCATTATCATTATTA+TGG - chr3_1:77451148-77451167 Msa0377070:intron 30.0%
CGATATATACCACTTATATG+TGG - chr3_1:77451194-77451213 Msa0377070:intron 30.0%
ACCATATAAGTGGCATATAT+CGG + chr3_1:77451226-77451245 None:intergenic 30.0%
GGGTTGTTATATTTCATTAC+AGG + chr3_1:77451282-77451301 None:intergenic 30.0%
AAGAGCCATGATTAATGAAA+TGG - chr3_1:77451335-77451354 Msa0377070:intron 30.0%
! TTTTATATTTTGTCGAGACG+TGG - chr3_1:77451595-77451614 Msa0377070:intron 30.0%
GATATATTATCAGCTTACCA+AGG - chr3_1:77451778-77451797 Msa0377070:exon 30.0%
GAAATTTGAAAGCAAGAACA+TGG + chr3_1:77451810-77451829 None:intergenic 30.0%
AGTATCTGTTAATCGTAAGA+AGG + chr3_1:77450466-77450485 None:intergenic 30.0%
TAACAGTTATCACTAAGACA+AGG + chr3_1:77450625-77450644 None:intergenic 30.0%
!! AGATTTGATTTTTCATGCTG+AGG - chr3_1:77450659-77450678 Msa0377070:exon 30.0%
! TATCAATTTTATGGTGGCTA+AGG - chr3_1:77450683-77450702 Msa0377070:CDS 30.0%
!! ATAGGTTTTTAAGAGAGACA+TGG + chr3_1:77451056-77451075 None:intergenic 30.0%
!! CATAATAATGATAATGGCAG+TGG + chr3_1:77451150-77451169 None:intergenic 30.0%
CACTGCCATTATCATTATTA+TGG - chr3_1:77451148-77451167 Msa0377070:intron 30.0%
CGATATATACCACTTATATG+TGG - chr3_1:77451194-77451213 Msa0377070:intron 30.0%
ACCATATAAGTGGCATATAT+CGG + chr3_1:77451226-77451245 None:intergenic 30.0%
GGGTTGTTATATTTCATTAC+AGG + chr3_1:77451282-77451301 None:intergenic 30.0%
AAGAGCCATGATTAATGAAA+TGG - chr3_1:77451335-77451354 Msa0377070:intron 30.0%
! TTTTATATTTTGTCGAGACG+TGG - chr3_1:77451595-77451614 Msa0377070:intron 30.0%
GATATATTATCAGCTTACCA+AGG - chr3_1:77451778-77451797 Msa0377070:exon 30.0%
GAAATTTGAAAGCAAGAACA+TGG + chr3_1:77451810-77451829 None:intergenic 30.0%
! TGCTGAGGCTATCAATTTTA+TGG - chr3_1:77450674-77450693 Msa0377070:exon 35.0%
! TGAGGCTATCAATTTTATGG+TGG - chr3_1:77450677-77450696 Msa0377070:CDS 35.0%
ATACTGTTCTGTTAGTGCTA+AGG - chr3_1:77450746-77450765 Msa0377070:CDS 35.0%
ATGTTTGACTACATTTCCGT+TGG - chr3_1:77450872-77450891 Msa0377070:intron 35.0%
! AGATAAAGGGGTCTTCTTTT+TGG + chr3_1:77450914-77450933 None:intergenic 35.0%
AAGAAGACCCCTTTATCTTA+TGG - chr3_1:77450916-77450935 Msa0377070:intron 35.0%
ACAAAGCTACAGTAGACTAT+AGG + chr3_1:77451074-77451093 None:intergenic 35.0%
! TCCGATATATGCCACTTATA+TGG - chr3_1:77451222-77451241 Msa0377070:intron 35.0%
TTTCATTACAGGGGAACAAT+AGG + chr3_1:77451271-77451290 None:intergenic 35.0%
!! TTTGTTGTTCTTCCTGCATT+TGG + chr3_1:77451317-77451336 None:intergenic 35.0%
AGCCATGATTAATGAAATGG+TGG - chr3_1:77451338-77451357 Msa0377070:intron 35.0%
TGCCACCATTTCATTAATCA+TGG + chr3_1:77451343-77451362 None:intergenic 35.0%
! AAAGATGACTAGTCAATGCT+GGG - chr3_1:77451362-77451381 Msa0377070:intron 35.0%
TGAGCATGCTTATCATGAAA+CGG - chr3_1:77451472-77451491 Msa0377070:intron 35.0%
GAGTATGCAATGAAGATCAA+CGG - chr3_1:77451500-77451519 Msa0377070:intron 35.0%
TTACTGCTTGTTTGACTGAA+GGG - chr3_1:77451683-77451702 Msa0377070:intron 35.0%
! TGCTGAGGCTATCAATTTTA+TGG - chr3_1:77450674-77450693 Msa0377070:exon 35.0%
! TGAGGCTATCAATTTTATGG+TGG - chr3_1:77450677-77450696 Msa0377070:CDS 35.0%
ATACTGTTCTGTTAGTGCTA+AGG - chr3_1:77450746-77450765 Msa0377070:CDS 35.0%
ATGTTTGACTACATTTCCGT+TGG - chr3_1:77450872-77450891 Msa0377070:intron 35.0%
! AGATAAAGGGGTCTTCTTTT+TGG + chr3_1:77450914-77450933 None:intergenic 35.0%
AAGAAGACCCCTTTATCTTA+TGG - chr3_1:77450916-77450935 Msa0377070:intron 35.0%
ACAAAGCTACAGTAGACTAT+AGG + chr3_1:77451074-77451093 None:intergenic 35.0%
! TCCGATATATGCCACTTATA+TGG - chr3_1:77451222-77451241 Msa0377070:intron 35.0%
TTTCATTACAGGGGAACAAT+AGG + chr3_1:77451271-77451290 None:intergenic 35.0%
!! TTTGTTGTTCTTCCTGCATT+TGG + chr3_1:77451317-77451336 None:intergenic 35.0%
AGCCATGATTAATGAAATGG+TGG - chr3_1:77451338-77451357 Msa0377070:intron 35.0%
TGCCACCATTTCATTAATCA+TGG + chr3_1:77451343-77451362 None:intergenic 35.0%
! AAAGATGACTAGTCAATGCT+GGG - chr3_1:77451362-77451381 Msa0377070:intron 35.0%
TGAGCATGCTTATCATGAAA+CGG - chr3_1:77451472-77451491 Msa0377070:intron 35.0%
GAGTATGCAATGAAGATCAA+CGG - chr3_1:77451500-77451519 Msa0377070:intron 35.0%
TTACTGCTTGTTTGACTGAA+GGG - chr3_1:77451683-77451702 Msa0377070:intron 35.0%
TTGTGCTGTGTTCGTTCATT+AGG - chr3_1:77450313-77450332 Msa0377070:three_prime_UTR 40.0%
TGTGCTGTGTTCGTTCATTA+GGG - chr3_1:77450314-77450333 Msa0377070:three_prime_UTR 40.0%
CATTTCAGCAGAATTGAGGT+CGG + chr3_1:77450410-77450429 None:intergenic 40.0%
TCTGCATTTCAGCAGAATTG+AGG + chr3_1:77450414-77450433 None:intergenic 40.0%
TCAATTCTGCTGAAATGCAG+AGG - chr3_1:77450413-77450432 Msa0377070:three_prime_UTR 40.0%
! ACATTTCCGTTGGTTAGACT+TGG - chr3_1:77450882-77450901 Msa0377070:intron 40.0%
AATCGACCAAGTCTAACCAA+CGG + chr3_1:77450891-77450910 None:intergenic 40.0%
ATGGAATTGGTACCCAAACA+CGG - chr3_1:77450995-77451014 Msa0377070:intron 40.0%
ATTATCTTCCCTCCGTGTTT+GGG + chr3_1:77451010-77451029 None:intergenic 40.0%
TATTATCTTCCCTCCGTGTT+TGG + chr3_1:77451011-77451030 None:intergenic 40.0%
TATGGCTTAGGGGATCTAAA+TGG - chr3_1:77451166-77451185 Msa0377070:intron 40.0%
CGGAATATGCCACATATAAG+TGG + chr3_1:77451206-77451225 None:intergenic 40.0%
GCATTTGGAGGGCAAAATTA+GGG + chr3_1:77451302-77451321 None:intergenic 40.0%
TGCATTTGGAGGGCAAAATT+AGG + chr3_1:77451303-77451322 None:intergenic 40.0%
! TAATTTTGCCCTCCAAATGC+AGG - chr3_1:77451302-77451321 Msa0377070:intron 40.0%
TTGTTCTTCCTGCATTTGGA+GGG + chr3_1:77451313-77451332 None:intergenic 40.0%
! CAAAGATGACTAGTCAATGC+TGG - chr3_1:77451361-77451380 Msa0377070:intron 40.0%
! TGTTAGGCAATTAGTCTCAC+CGG + chr3_1:77451427-77451446 None:intergenic 40.0%
CTTACTGCTTGTTTGACTGA+AGG - chr3_1:77451682-77451701 Msa0377070:intron 40.0%
TTGTGCTGTGTTCGTTCATT+AGG - chr3_1:77450313-77450332 Msa0377070:three_prime_UTR 40.0%
TGTGCTGTGTTCGTTCATTA+GGG - chr3_1:77450314-77450333 Msa0377070:three_prime_UTR 40.0%
CATTTCAGCAGAATTGAGGT+CGG + chr3_1:77450410-77450429 None:intergenic 40.0%
TCTGCATTTCAGCAGAATTG+AGG + chr3_1:77450414-77450433 None:intergenic 40.0%
TCAATTCTGCTGAAATGCAG+AGG - chr3_1:77450413-77450432 Msa0377070:three_prime_UTR 40.0%
! ACATTTCCGTTGGTTAGACT+TGG - chr3_1:77450882-77450901 Msa0377070:intron 40.0%
AATCGACCAAGTCTAACCAA+CGG + chr3_1:77450891-77450910 None:intergenic 40.0%
ATGGAATTGGTACCCAAACA+CGG - chr3_1:77450995-77451014 Msa0377070:intron 40.0%
ATTATCTTCCCTCCGTGTTT+GGG + chr3_1:77451010-77451029 None:intergenic 40.0%
TATTATCTTCCCTCCGTGTT+TGG + chr3_1:77451011-77451030 None:intergenic 40.0%
TATGGCTTAGGGGATCTAAA+TGG - chr3_1:77451166-77451185 Msa0377070:intron 40.0%
CGGAATATGCCACATATAAG+TGG + chr3_1:77451206-77451225 None:intergenic 40.0%
GCATTTGGAGGGCAAAATTA+GGG + chr3_1:77451302-77451321 None:intergenic 40.0%
TGCATTTGGAGGGCAAAATT+AGG + chr3_1:77451303-77451322 None:intergenic 40.0%
! TAATTTTGCCCTCCAAATGC+AGG - chr3_1:77451302-77451321 Msa0377070:intron 40.0%
TTGTTCTTCCTGCATTTGGA+GGG + chr3_1:77451313-77451332 None:intergenic 40.0%
! CAAAGATGACTAGTCAATGC+TGG - chr3_1:77451361-77451380 Msa0377070:intron 40.0%
! TGTTAGGCAATTAGTCTCAC+CGG + chr3_1:77451427-77451446 None:intergenic 40.0%
CTTACTGCTTGTTTGACTGA+AGG - chr3_1:77451682-77451701 Msa0377070:intron 40.0%
ATTGAGAGAGAGCGATTGAG+AGG + chr3_1:77450379-77450398 None:intergenic 45.0%
TCTCAATCGCTCTCTCTCAA+TGG - chr3_1:77450378-77450397 Msa0377070:three_prime_UTR 45.0%
!!! TGCTAAGGCCAGTTGGTTTT+TGG - chr3_1:77450761-77450780 Msa0377070:CDS 45.0%
!!! TAAGGCCAGTTGGTTTTTGG+AGG - chr3_1:77450764-77450783 Msa0377070:CDS 45.0%
!!! AAGGCCAGTTGGTTTTTGGA+GGG - chr3_1:77450765-77450784 Msa0377070:CDS 45.0%
! ATTTGGTTCACTTCAGCTGC+TGG - chr3_1:77450823-77450842 Msa0377070:CDS 45.0%
AATTGGTACCCAAACACGGA+GGG - chr3_1:77450999-77451018 Msa0377070:intron 45.0%
AAGAGAGACATGGCAAACAG+TGG + chr3_1:77451046-77451065 None:intergenic 45.0%
GTTGTTCTTCCTGCATTTGG+AGG + chr3_1:77451314-77451333 None:intergenic 45.0%
CCTGGGAATAAGTTCAGTTC+CGG - chr3_1:77451405-77451424 Msa0377070:intron 45.0%
GATGAAAAGCTCAGTGCATG+TGG - chr3_1:77451652-77451671 Msa0377070:intron 45.0%
ATTTCTTGTTCGTGTGCCGA+AGG - chr3_1:77451860-77451879 Msa0377070:five_prime_UTR 45.0%
ATTGAGAGAGAGCGATTGAG+AGG + chr3_1:77450379-77450398 None:intergenic 45.0%
TCTCAATCGCTCTCTCTCAA+TGG - chr3_1:77450378-77450397 Msa0377070:three_prime_UTR 45.0%
!!! TGCTAAGGCCAGTTGGTTTT+TGG - chr3_1:77450761-77450780 Msa0377070:CDS 45.0%
!!! TAAGGCCAGTTGGTTTTTGG+AGG - chr3_1:77450764-77450783 Msa0377070:CDS 45.0%
!!! AAGGCCAGTTGGTTTTTGGA+GGG - chr3_1:77450765-77450784 Msa0377070:CDS 45.0%
! ATTTGGTTCACTTCAGCTGC+TGG - chr3_1:77450823-77450842 Msa0377070:CDS 45.0%
AATTGGTACCCAAACACGGA+GGG - chr3_1:77450999-77451018 Msa0377070:intron 45.0%
AAGAGAGACATGGCAAACAG+TGG + chr3_1:77451046-77451065 None:intergenic 45.0%
GTTGTTCTTCCTGCATTTGG+AGG + chr3_1:77451314-77451333 None:intergenic 45.0%
CCTGGGAATAAGTTCAGTTC+CGG - chr3_1:77451405-77451424 Msa0377070:intron 45.0%
GATGAAAAGCTCAGTGCATG+TGG - chr3_1:77451652-77451671 Msa0377070:intron 45.0%
ATTTCTTGTTCGTGTGCCGA+AGG - chr3_1:77451860-77451879 Msa0377070:five_prime_UTR 45.0%
ATTGAGAGGAGAGAGAGAGG+GGG + chr3_1:77450365-77450384 None:intergenic 50.0%
GATTGAGAGGAGAGAGAGAG+GGG + chr3_1:77450366-77450385 None:intergenic 50.0%
CGATTGAGAGGAGAGAGAGA+GGG + chr3_1:77450367-77450386 None:intergenic 50.0%
CTGTTAGTGCTAAGGCCAGT+TGG - chr3_1:77450754-77450773 Msa0377070:CDS 50.0%
!!! AGGCCAGTTGGTTTTTGGAG+GGG - chr3_1:77450766-77450785 Msa0377070:CDS 50.0%
!!! CAGTTGGTTTTTGGAGGGGA+CGG - chr3_1:77450770-77450789 Msa0377070:CDS 50.0%
GAATTGGTACCCAAACACGG+AGG - chr3_1:77450998-77451017 Msa0377070:intron 50.0%
TGCTGGGACAAATGCATCAC+AGG - chr3_1:77451378-77451397 Msa0377070:intron 50.0%
CCGGAACTGAACTTATTCCC+AGG + chr3_1:77451408-77451427 None:intergenic 50.0%
AACGCTGTGCACAGTGTGTT+AGG + chr3_1:77451443-77451462 None:intergenic 50.0%
ACACTGTGCACAGCGTTACA+TGG - chr3_1:77451446-77451465 Msa0377070:intron 50.0%
CAAGAACATGGCAAGACCCT+TGG + chr3_1:77451798-77451817 None:intergenic 50.0%
ATTGAGAGGAGAGAGAGAGG+GGG + chr3_1:77450365-77450384 None:intergenic 50.0%
GATTGAGAGGAGAGAGAGAG+GGG + chr3_1:77450366-77450385 None:intergenic 50.0%
CGATTGAGAGGAGAGAGAGA+GGG + chr3_1:77450367-77450386 None:intergenic 50.0%
CTGTTAGTGCTAAGGCCAGT+TGG - chr3_1:77450754-77450773 Msa0377070:CDS 50.0%
!!! AGGCCAGTTGGTTTTTGGAG+GGG - chr3_1:77450766-77450785 Msa0377070:CDS 50.0%
!!! CAGTTGGTTTTTGGAGGGGA+CGG - chr3_1:77450770-77450789 Msa0377070:CDS 50.0%
GAATTGGTACCCAAACACGG+AGG - chr3_1:77450998-77451017 Msa0377070:intron 50.0%
TGCTGGGACAAATGCATCAC+AGG - chr3_1:77451378-77451397 Msa0377070:intron 50.0%
CCGGAACTGAACTTATTCCC+AGG + chr3_1:77451408-77451427 None:intergenic 50.0%
AACGCTGTGCACAGTGTGTT+AGG + chr3_1:77451443-77451462 None:intergenic 50.0%
ACACTGTGCACAGCGTTACA+TGG - chr3_1:77451446-77451465 Msa0377070:intron 50.0%
CAAGAACATGGCAAGACCCT+TGG + chr3_1:77451798-77451817 None:intergenic 50.0%
GCGATTGAGAGGAGAGAGAG+AGG + chr3_1:77450368-77450387 None:intergenic 55.0%
CGTCCCCTCCAAAAACCAAC+TGG + chr3_1:77450772-77450791 None:intergenic 55.0%
!! GTTTTTGGAGGGGACGGCTA+AGG - chr3_1:77450776-77450795 Msa0377070:CDS 55.0%
AAATGCATCACAGGCACGCC+TGG - chr3_1:77451387-77451406 Msa0377070:intron 55.0%
AATGCATCACAGGCACGCCT+GGG - chr3_1:77451388-77451407 Msa0377070:intron 55.0%
GCGATTGAGAGGAGAGAGAG+AGG + chr3_1:77450368-77450387 None:intergenic 55.0%
CGTCCCCTCCAAAAACCAAC+TGG + chr3_1:77450772-77450791 None:intergenic 55.0%
!! GTTTTTGGAGGGGACGGCTA+AGG - chr3_1:77450776-77450795 Msa0377070:CDS 55.0%
AAATGCATCACAGGCACGCC+TGG - chr3_1:77451387-77451406 Msa0377070:intron 55.0%
AATGCATCACAGGCACGCCT+GGG - chr3_1:77451388-77451407 Msa0377070:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 77450301 77451888 77450301 ID=Msa0377070;Name=Msa0377070
chr3_1 mRNA 77450301 77451888 77450301 ID=Msa0377070-mRNA-1;Parent=Msa0377070;Name=Msa0377070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|1|1|1|1|1|2|376|78
chr3_1 exon 77450301 77450865 77450301 ID=Msa0377070-mRNA-1:exon:18532;Parent=Msa0377070-mRNA-1
chr3_1 exon 77451745 77451888 77451745 ID=Msa0377070-mRNA-1:exon:18531;Parent=Msa0377070-mRNA-1
chr3_1 five_prime_UTR 77451793 77451888 77451793 ID=Msa0377070-mRNA-1:five_prime_utr;Parent=Msa0377070-mRNA-1
chr3_1 CDS 77451745 77451792 77451745 ID=Msa0377070-mRNA-1:cds;Parent=Msa0377070-mRNA-1
chr3_1 CDS 77450677 77450865 77450677 ID=Msa0377070-mRNA-1:cds;Parent=Msa0377070-mRNA-1
chr3_1 three_prime_UTR 77450301 77450676 77450301 ID=Msa0377070-mRNA-1:three_prime_utr;Parent=Msa0377070-mRNA-1
Gene Sequence

>Msa0377070

ATGGATCTTTCCGACCTCAATTCTGCTGAAATGCAGAGGTTTTACTCTGAAGAACAACAAAGAGCCATGATTAATGAAATGGTGGCAAAGATGACTAGTCAATGCTGGGACAAATGCATCACAGGCACGCCTGGGAATAAGTTCAGTTCCGGTGAGACTAATTGCCTAACACACTGTGCACAGCGTTACATGGAGATGAGCATGCTTATCATGAAACGGTTTCAGAGTATGCAATGA
Protein sequence

>Msa0377070

MDLSDLNSAEMQRFYSEEQQRAMINEMVAKMTSQCWDKCITGTPGNKFSSGETNCLTHCAQRYMEMSMLIMKRFQSMQ*