AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0377750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0377750 MtrunA17_Chr3g0128761 92.746 193 14 0 17 209 10 202 5.82e-118 333
Msa0377750 MtrunA17_Chr1g0213011 31.604 212 133 5 6 209 8 215 1.03e-20 86.3
Msa0377750 MtrunA17_Chr1g0213031 27.536 207 140 4 6 205 11 214 1.47e-19 83.2
Msa0377750 MtrunA17_Chr1g0213021 27.273 209 142 4 6 207 11 216 1.00e-18 80.9
Msa0377750 MtrunA17_Chr1g0213051 27.907 215 144 6 1 205 1 214 2.06e-18 80.1
Msa0377750 MtrunA17_Chr1g0213041 29.767 215 140 6 1 205 1 214 2.15e-17 77.4
Msa0377750 MtrunA17_Chr2g0313901 28.000 200 133 6 10 207 86 276 2.89e-16 75.1
Msa0377750 MtrunA17_Chr7g0224381 29.412 153 101 3 1 146 1 153 3.52e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0377750 AT5G05270.1 62.679 209 77 1 1 208 1 209 4.56e-83 246
Msa0377750 AT5G05270.2 62.679 209 77 1 1 208 1 209 4.56e-83 246
Msa0377750 AT3G55120.1 30.841 214 128 8 8 209 21 226 2.92e-17 77.8
Msa0377750 AT1G53520.1 26.882 186 130 4 23 207 106 286 6.97e-17 77.4
Msa0377750 AT5G66230.2 29.048 210 129 7 8 209 11 208 5.79e-15 73.2
Msa0377750 AT5G66220.1 29.032 155 102 3 59 209 9 159 2.38e-13 65.9

Find 77 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTCAATTGGGGTTTATT+TGG 0.127513 3_1:-78377826 Msa0377750:CDS
AGAATTTCACTTGGAGAAAA+TGG 0.134571 3_1:+78377847 None:intergenic
TTCTTTGACGCTCTCATTTC+TGG 0.168815 3_1:-78377732 Msa0377750:intron
TGTTGTGATTAAAGAGATTA+AGG 0.213746 3_1:-78377483 Msa0377750:CDS
TCCTTCAACCATTACATCTT+TGG 0.255062 3_1:-78376814 Msa0377750:CDS
ATTGCTTAGGTTGTTATATA+AGG 0.261912 3_1:+78378456 None:intergenic
AATAAATTGTTATTCTTTGT+TGG 0.283400 3_1:-78376606 Msa0377750:three_prime_UTR
CAAGTGAAATTCTATTCAAT+TGG 0.286514 3_1:-78377837 Msa0377750:CDS
AATACAGATTGTGGTATCTT+TGG 0.300414 3_1:-78376928 Msa0377750:intron
TGCAAGTGGCTTACTACTTC+AGG 0.306819 3_1:+78377801 None:intergenic
CACACTCCGTAGCTAAATTA+AGG 0.334675 3_1:+78378491 None:intergenic
AAGTGAAATTCTATTCAATT+GGG 0.340514 3_1:-78377836 Msa0377750:CDS
AGTGGCTAGTGAAACTGTTT+TGG 0.373529 3_1:-78378214 Msa0377750:CDS
CATTGCACGTGTGGAATCTA+TGG 0.382464 3_1:+78376577 None:intergenic
GAGATTAAGGGTGCACAATA+TGG 0.383753 3_1:-78377470 Msa0377750:CDS
GGATGAAGAAGAAGAAGCTT+TGG 0.390087 3_1:-78377393 Msa0377750:CDS
GACTTTCTCCACTTTGTTTG+CGG 0.390887 3_1:-78376695 Msa0377750:three_prime_UTR
AGCTCTTACTAGTTAGTTGC+TGG 0.395872 3_1:+78378341 None:intergenic
TTGTCTTCCAACTCCTTGGC+AGG 0.403272 3_1:+78377762 None:intergenic
ATTAAGAAATGGTATCTTGC+TGG 0.410454 3_1:-78376852 Msa0377750:CDS
ACCAAGGAGAGAAAGGGTTT+TGG 0.417350 3_1:+78378155 None:intergenic
TCAGCAGTTGGAGAGTTTGC+TGG 0.419031 3_1:+78377305 None:intergenic
GACCGCTGTGAGGGACCGTT+TGG 0.422176 3_1:-78377435 Msa0377750:CDS
ACCAAAACCCTTTCTCTCCT+TGG 0.424712 3_1:-78378156 Msa0377750:CDS
TTGGTAGTAGTGATCTTTGA+AGG 0.439710 3_1:+78378174 None:intergenic
GTTGTGATTAAAGAGATTAA+GGG 0.453545 3_1:-78377482 Msa0377750:CDS
TAGAGAGAATTGTGGTGAGA+TGG 0.457933 3_1:+78378392 None:intergenic
TTCATCCTCATATTTGTCAT+CGG 0.460901 3_1:+78377409 None:intergenic
ATCATTGTCTTCCAACTCCT+TGG 0.461782 3_1:+78377758 None:intergenic
TAGTAGAGACTATTAAGAAA+TGG 0.472191 3_1:-78376863 Msa0377750:CDS
GTTTCTTGAAGTACTTAGAC+TGG 0.476418 3_1:+78377351 None:intergenic
TACCATGACCAAGGAGAGAA+AGG 0.488761 3_1:+78378148 None:intergenic
TCAAAGTATGTAATAGAGAA+TGG 0.503420 3_1:-78376891 Msa0377750:CDS
GCCAAAGATGTAATGGTTGA+AGG 0.506201 3_1:+78376813 None:intergenic
CTTAAGATGAGATGTAAGAA+TGG 0.508372 3_1:+78378428 None:intergenic
CAGCAGTTGGAGAGTTTGCT+GGG 0.511362 3_1:+78377306 None:intergenic
TGGATAATTCCTCTGAGAGA+TGG 0.511898 3_1:+78376787 None:intergenic
CAATTGAATAGAATTTCACT+TGG 0.511986 3_1:+78377838 None:intergenic
AAGAATGGTAGATATTGCTT+AGG 0.517256 3_1:+78378443 None:intergenic
AGCTACTTTACCTCAGCAGT+TGG 0.518480 3_1:+78377293 None:intergenic
AGTCTAATTGCCTTCTCCAC+AGG 0.518956 3_1:+78377506 None:intergenic
ACTTGTGACTTTCTTTCAAG+TGG 0.521102 3_1:+78378537 None:intergenic
CAGATTGTGGTATCTTTGGA+AGG 0.521938 3_1:-78376924 Msa0377750:CDS
TACCTTTCCACTGTTGCAAG+TGG 0.522349 3_1:+78377787 None:intergenic
ATGTGCAGCTCCTGTGGAGA+AGG 0.525875 3_1:-78377516 Msa0377750:intron
AGAGGAAGTAGAGAGAATTG+TGG 0.528018 3_1:+78378384 None:intergenic
ATGGGTAGCCAAAGATGTAA+TGG 0.528630 3_1:+78376806 None:intergenic
ATAGTTTGCCATTGCACGTG+TGG 0.537970 3_1:+78376568 None:intergenic
GGATAATTCCTCTGAGAGAT+GGG 0.538139 3_1:+78376788 None:intergenic
ACCATGACCAAGGAGAGAAA+GGG 0.540135 3_1:+78378149 None:intergenic
ACCCTTTCTCTCCTTGGTCA+TGG 0.540415 3_1:-78378150 Msa0377750:intron
GTGAATATGTGCAGCTCCTG+TGG 0.541590 3_1:-78377522 Msa0377750:intron
AATGGTATCTTGCTGGCTCA+AGG 0.546466 3_1:-78376845 Msa0377750:CDS
CTACCACCTTAATTTAGCTA+CGG 0.556695 3_1:-78378497 Msa0377750:five_prime_UTR
CATAGATTCCACACGTGCAA+TGG 0.557143 3_1:-78376576 Msa0377750:three_prime_UTR
ATTTGTCATCGGCTGCCAAA+CGG 0.557673 3_1:+78377420 None:intergenic
AGTGAAATTCTATTCAATTG+GGG 0.558547 3_1:-78377835 Msa0377750:CDS
TGGTAGTAGTGATCTTTGAA+GGG 0.566326 3_1:+78378175 None:intergenic
ATGGTATCTTGCTGGCTCAA+GGG 0.567607 3_1:-78376844 Msa0377750:CDS
AGGTAAGCCTGCCAAGGAGT+TGG 0.569479 3_1:-78377769 Msa0377750:CDS
TAGTAAGCCACTTGCAACAG+TGG 0.573061 3_1:-78377794 Msa0377750:CDS
GGCAGCCGATGACAAATATG+AGG 0.581635 3_1:-78377414 Msa0377750:CDS
CGTAGCTAAATTAAGGTGGT+AGG 0.585339 3_1:+78378498 None:intergenic
GCAGAATTCCGCAAACAAAG+TGG 0.588511 3_1:+78376687 None:intergenic
TTTGATAGGGATTACCGACA+TGG 0.591441 3_1:-78377874 Msa0377750:intron
ATCGATGTAGATAGAGACAG+AGG 0.592514 3_1:+78378366 None:intergenic
AGCCACTTGCAACAGTGGAA+AGG 0.600159 3_1:-78377789 Msa0377750:CDS
AGATTGTGGTATCTTTGGAA+GGG 0.601655 3_1:-78376923 Msa0377750:CDS
GTGGAAAGGTAAGCCTGCCA+AGG 0.623835 3_1:-78377775 Msa0377750:CDS
GAGAAAATGGATCTCCATGT+CGG 0.629435 3_1:+78377860 None:intergenic
TCAGATTGAGACCGCTGTGA+GGG 0.629678 3_1:-78377444 Msa0377750:CDS
AGCAAACTCTCCAACTGCTG+AGG 0.645259 3_1:-78377303 Msa0377750:intron
TGCCAAACGGTCCCTCACAG+CGG 0.689378 3_1:+78377433 None:intergenic
TTTGGCTACCCATCTCTCAG+AGG 0.689828 3_1:-78376796 Msa0377750:CDS
TTCAGATTGAGACCGCTGTG+AGG 0.696200 3_1:-78377445 Msa0377750:CDS
ACTCCGTAGCTAAATTAAGG+TGG 0.699822 3_1:+78378494 None:intergenic
GTAGCAACTTACCATGACCA+AGG 0.712424 3_1:+78378139 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGTTTAATAATAATGT+TGG + chr3_1:78376596-78376615 None:intergenic 10.0%
!!! TTTTTGTTTAATAATAATGT+TGG + chr3_1:78376596-78376615 None:intergenic 10.0%
!! CAACATTTATGATATAATTA+AGG + chr3_1:78377585-78377604 None:intergenic 15.0%
!!! AATTAGTTTAAGTTGATTTT+AGG - chr3_1:78377938-78377957 Msa0377750:intron 15.0%
!! TTTAAAGAAAAAGTTTAAGT+TGG + chr3_1:78378007-78378026 None:intergenic 15.0%
!! AAAAATAAATAAATTGAGGA+AGG - chr3_1:78378025-78378044 Msa0377750:intron 15.0%
!!! AATTCTGCTTTTTAATTTAT+TGG - chr3_1:78378489-78378508 Msa0377750:five_prime_UTR 15.0%
!!! AATAAATTGTTATTCTTTGT+TGG - chr3_1:78378555-78378574 Msa0377750:five_prime_UTR 15.0%
!! CAACATTTATGATATAATTA+AGG + chr3_1:78377585-78377604 None:intergenic 15.0%
!!! AATTAGTTTAAGTTGATTTT+AGG - chr3_1:78377938-78377957 Msa0377750:intron 15.0%
!! TTTAAAGAAAAAGTTTAAGT+TGG + chr3_1:78378007-78378026 None:intergenic 15.0%
!! AAAAATAAATAAATTGAGGA+AGG - chr3_1:78378025-78378044 Msa0377750:intron 15.0%
!!! AATTCTGCTTTTTAATTTAT+TGG - chr3_1:78378489-78378508 Msa0377750:five_prime_UTR 15.0%
!!! AATAAATTGTTATTCTTTGT+TGG - chr3_1:78378555-78378574 Msa0377750:five_prime_UTR 15.0%
!! TCAAATCAAGAAAAAGAATT+TGG + chr3_1:78377076-78377095 None:intergenic 20.0%
!!! TCTTGATTTGATTTTGAATA+TGG - chr3_1:78377085-78377104 Msa0377750:intron 20.0%
!!! CTTGATTTGATTTTGAATAT+GGG - chr3_1:78377086-78377105 Msa0377750:intron 20.0%
!!! TTGATTTGATTTTGAATATG+GGG - chr3_1:78377087-78377106 Msa0377750:intron 20.0%
!! AAGTGAAATTCTATTCAATT+GGG - chr3_1:78377325-78377344 Msa0377750:CDS 20.0%
!! AAAGAAGTTGGATTATAAAA+GGG + chr3_1:78377466-78377485 None:intergenic 20.0%
!!! AACTAATTCTAAGAATGAAT+AGG + chr3_1:78377926-78377945 None:intergenic 20.0%
!!! AAAAAGTTTAAGTTGGTTAT+AGG + chr3_1:78378000-78378019 None:intergenic 20.0%
!! TTGTTAATATTAGTTGAAGT+AGG + chr3_1:78378172-78378191 None:intergenic 20.0%
!! TCAAATCAAGAAAAAGAATT+TGG + chr3_1:78377076-78377095 None:intergenic 20.0%
!!! TCTTGATTTGATTTTGAATA+TGG - chr3_1:78377085-78377104 Msa0377750:intron 20.0%
!!! CTTGATTTGATTTTGAATAT+GGG - chr3_1:78377086-78377105 Msa0377750:intron 20.0%
!!! TTGATTTGATTTTGAATATG+GGG - chr3_1:78377087-78377106 Msa0377750:intron 20.0%
!! AAGTGAAATTCTATTCAATT+GGG - chr3_1:78377325-78377344 Msa0377750:CDS 20.0%
!! AAAGAAGTTGGATTATAAAA+GGG + chr3_1:78377466-78377485 None:intergenic 20.0%
!!! AACTAATTCTAAGAATGAAT+AGG + chr3_1:78377926-78377945 None:intergenic 20.0%
!!! AAAAAGTTTAAGTTGGTTAT+AGG + chr3_1:78378000-78378019 None:intergenic 20.0%
!! TTGTTAATATTAGTTGAAGT+AGG + chr3_1:78378172-78378191 None:intergenic 20.0%
! CATTTGTAAAAAAAGAACGT+AGG - chr3_1:78376550-78376569 Msa0377750:three_prime_UTR 25.0%
!! ATTGCTTAGGTTGTTATATA+AGG + chr3_1:78376708-78376727 None:intergenic 25.0%
! GAATTTGGAAGAATTTCAAA+AGG + chr3_1:78377061-78377080 None:intergenic 25.0%
!! GGAAAATCAAGTTATTGATT+TGG + chr3_1:78377264-78377283 None:intergenic 25.0%
!! TTGATTTTCCTCTTTTGATA+GGG - chr3_1:78377274-78377293 Msa0377750:intron 25.0%
! CAATTGAATAGAATTTCACT+TGG + chr3_1:78377326-78377345 None:intergenic 25.0%
! CAAGTGAAATTCTATTCAAT+TGG - chr3_1:78377324-78377343 Msa0377750:CDS 25.0%
! AGTGAAATTCTATTCAATTG+GGG - chr3_1:78377326-78377345 Msa0377750:CDS 25.0%
! GAAAGAAGTTGGATTATAAA+AGG + chr3_1:78377467-78377486 None:intergenic 25.0%
! AAGATATTATCGAAAGAAGT+TGG + chr3_1:78377478-78377497 None:intergenic 25.0%
! TGTTGTGATTAAAGAGATTA+AGG - chr3_1:78377678-78377697 Msa0377750:intron 25.0%
! GTTGTGATTAAAGAGATTAA+GGG - chr3_1:78377679-78377698 Msa0377750:intron 25.0%
! AGAATGAATAGGATCAATTA+AGG + chr3_1:78377915-78377934 None:intergenic 25.0%
! TATGAGATTTGAATTTCATC+CGG - chr3_1:78378101-78378120 Msa0377750:intron 25.0%
!!! TAAGAGTTTGATTTTGAAAC+CGG + chr3_1:78378123-78378142 None:intergenic 25.0%
! GTTTCAAAATCAAACTCTTA+CGG - chr3_1:78378123-78378142 Msa0377750:intron 25.0%
! TCAAAGTATGTAATAGAGAA+TGG - chr3_1:78378270-78378289 Msa0377750:intron 25.0%
! TAGTAGAGACTATTAAGAAA+TGG - chr3_1:78378298-78378317 Msa0377750:intron 25.0%
!!! TGTTGCTATTTTGTACTAAT+TGG - chr3_1:78378443-78378462 Msa0377750:five_prime_UTR 25.0%
!!! GTTGCTATTTTGTACTAATT+GGG - chr3_1:78378444-78378463 Msa0377750:five_prime_UTR 25.0%
! CATTTGTAAAAAAAGAACGT+AGG - chr3_1:78376550-78376569 Msa0377750:three_prime_UTR 25.0%
!! ATTGCTTAGGTTGTTATATA+AGG + chr3_1:78376708-78376727 None:intergenic 25.0%
! GAATTTGGAAGAATTTCAAA+AGG + chr3_1:78377061-78377080 None:intergenic 25.0%
!! GGAAAATCAAGTTATTGATT+TGG + chr3_1:78377264-78377283 None:intergenic 25.0%
!! TTGATTTTCCTCTTTTGATA+GGG - chr3_1:78377274-78377293 Msa0377750:intron 25.0%
! CAATTGAATAGAATTTCACT+TGG + chr3_1:78377326-78377345 None:intergenic 25.0%
! CAAGTGAAATTCTATTCAAT+TGG - chr3_1:78377324-78377343 Msa0377750:CDS 25.0%
! AGTGAAATTCTATTCAATTG+GGG - chr3_1:78377326-78377345 Msa0377750:CDS 25.0%
! GAAAGAAGTTGGATTATAAA+AGG + chr3_1:78377467-78377486 None:intergenic 25.0%
! AAGATATTATCGAAAGAAGT+TGG + chr3_1:78377478-78377497 None:intergenic 25.0%
! TGTTGTGATTAAAGAGATTA+AGG - chr3_1:78377678-78377697 Msa0377750:intron 25.0%
! GTTGTGATTAAAGAGATTAA+GGG - chr3_1:78377679-78377698 Msa0377750:intron 25.0%
! AGAATGAATAGGATCAATTA+AGG + chr3_1:78377915-78377934 None:intergenic 25.0%
! TATGAGATTTGAATTTCATC+CGG - chr3_1:78378101-78378120 Msa0377750:intron 25.0%
!!! TAAGAGTTTGATTTTGAAAC+CGG + chr3_1:78378123-78378142 None:intergenic 25.0%
! GTTTCAAAATCAAACTCTTA+CGG - chr3_1:78378123-78378142 Msa0377750:intron 25.0%
! TCAAAGTATGTAATAGAGAA+TGG - chr3_1:78378270-78378289 Msa0377750:intron 25.0%
! TAGTAGAGACTATTAAGAAA+TGG - chr3_1:78378298-78378317 Msa0377750:intron 25.0%
!!! TGTTGCTATTTTGTACTAAT+TGG - chr3_1:78378443-78378462 Msa0377750:five_prime_UTR 25.0%
!!! GTTGCTATTTTGTACTAATT+GGG - chr3_1:78378444-78378463 Msa0377750:five_prime_UTR 25.0%
AAAAAAGAACGTAGGAGTTT+AGG - chr3_1:78376558-78376577 Msa0377750:three_prime_UTR 30.0%
AAGAATGGTAGATATTGCTT+AGG + chr3_1:78376721-78376740 None:intergenic 30.0%
CTTAAGATGAGATGTAAGAA+TGG + chr3_1:78376736-78376755 None:intergenic 30.0%
GTTTGTGAATTTGATTACAG+TGG - chr3_1:78376929-78376948 Msa0377750:intron 30.0%
! CTTGATTTTCCTCTTTTGAT+AGG - chr3_1:78377273-78377292 Msa0377750:intron 30.0%
AGAATTTCACTTGGAGAAAA+TGG + chr3_1:78377317-78377336 None:intergenic 30.0%
CTATTCAATTGGGGTTTATT+TGG - chr3_1:78377335-78377354 Msa0377750:CDS 30.0%
GAGCTCGTCTATTTATTATT+TGG - chr3_1:78377533-78377552 Msa0377750:intron 30.0%
TTCATCCTCATATTTGTCAT+CGG + chr3_1:78377755-78377774 None:intergenic 30.0%
!! AGTTGATTTTAGGTACAAGT+TGG - chr3_1:78377948-78377967 Msa0377750:intron 30.0%
!! GTTTGTTGTAATACAGATTG+TGG - chr3_1:78378224-78378243 Msa0377750:intron 30.0%
AATACAGATTGTGGTATCTT+TGG - chr3_1:78378233-78378252 Msa0377750:intron 30.0%
ATTAAGAAATGGTATCTTGC+TGG - chr3_1:78378309-78378328 Msa0377750:intron 30.0%
! AACACAAAGTTTTCTTCACT+TGG + chr3_1:78378397-78378416 None:intergenic 30.0%
AAAAAAGAACGTAGGAGTTT+AGG - chr3_1:78376558-78376577 Msa0377750:three_prime_UTR 30.0%
AAGAATGGTAGATATTGCTT+AGG + chr3_1:78376721-78376740 None:intergenic 30.0%
CTTAAGATGAGATGTAAGAA+TGG + chr3_1:78376736-78376755 None:intergenic 30.0%
GTTTGTGAATTTGATTACAG+TGG - chr3_1:78376929-78376948 Msa0377750:intron 30.0%
! CTTGATTTTCCTCTTTTGAT+AGG - chr3_1:78377273-78377292 Msa0377750:intron 30.0%
AGAATTTCACTTGGAGAAAA+TGG + chr3_1:78377317-78377336 None:intergenic 30.0%
CTATTCAATTGGGGTTTATT+TGG - chr3_1:78377335-78377354 Msa0377750:CDS 30.0%
GAGCTCGTCTATTTATTATT+TGG - chr3_1:78377533-78377552 Msa0377750:intron 30.0%
TTCATCCTCATATTTGTCAT+CGG + chr3_1:78377755-78377774 None:intergenic 30.0%
!! AGTTGATTTTAGGTACAAGT+TGG - chr3_1:78377948-78377967 Msa0377750:intron 30.0%
!! GTTTGTTGTAATACAGATTG+TGG - chr3_1:78378224-78378243 Msa0377750:intron 30.0%
AATACAGATTGTGGTATCTT+TGG - chr3_1:78378233-78378252 Msa0377750:intron 30.0%
ATTAAGAAATGGTATCTTGC+TGG - chr3_1:78378309-78378328 Msa0377750:intron 30.0%
! AACACAAAGTTTTCTTCACT+TGG + chr3_1:78378397-78378416 None:intergenic 30.0%
ACTTGTGACTTTCTTTCAAG+TGG + chr3_1:78376627-78376646 None:intergenic 35.0%
CTACCACCTTAATTTAGCTA+CGG - chr3_1:78376664-78376683 Msa0377750:three_prime_UTR 35.0%
TGGTAGTAGTGATCTTTGAA+GGG + chr3_1:78376989-78377008 None:intergenic 35.0%
! TTGGTAGTAGTGATCTTTGA+AGG + chr3_1:78376990-78377009 None:intergenic 35.0%
CTAAAAGTAGCAAGAGATGT+AGG - chr3_1:78377204-78377223 Msa0377750:intron 35.0%
! GTTTCTTGAAGTACTTAGAC+TGG + chr3_1:78377813-78377832 None:intergenic 35.0%
!!! GATTTTAGGTACAAGTTGGT+TGG - chr3_1:78377952-78377971 Msa0377750:intron 35.0%
! AGATTGTGGTATCTTTGGAA+GGG - chr3_1:78378238-78378257 Msa0377750:intron 35.0%
TCCTTCAACCATTACATCTT+TGG - chr3_1:78378347-78378366 Msa0377750:intron 35.0%
ACTTGTGACTTTCTTTCAAG+TGG + chr3_1:78376627-78376646 None:intergenic 35.0%
CTACCACCTTAATTTAGCTA+CGG - chr3_1:78376664-78376683 Msa0377750:three_prime_UTR 35.0%
TGGTAGTAGTGATCTTTGAA+GGG + chr3_1:78376989-78377008 None:intergenic 35.0%
! TTGGTAGTAGTGATCTTTGA+AGG + chr3_1:78376990-78377009 None:intergenic 35.0%
CTAAAAGTAGCAAGAGATGT+AGG - chr3_1:78377204-78377223 Msa0377750:intron 35.0%
! GTTTCTTGAAGTACTTAGAC+TGG + chr3_1:78377813-78377832 None:intergenic 35.0%
!!! GATTTTAGGTACAAGTTGGT+TGG - chr3_1:78377952-78377971 Msa0377750:intron 35.0%
! AGATTGTGGTATCTTTGGAA+GGG - chr3_1:78378238-78378257 Msa0377750:intron 35.0%
TCCTTCAACCATTACATCTT+TGG - chr3_1:78378347-78378366 Msa0377750:intron 35.0%
CGTAGCTAAATTAAGGTGGT+AGG + chr3_1:78376666-78376685 None:intergenic 40.0%
ACTCCGTAGCTAAATTAAGG+TGG + chr3_1:78376670-78376689 None:intergenic 40.0%
CACACTCCGTAGCTAAATTA+AGG + chr3_1:78376673-78376692 None:intergenic 40.0%
TAGAGAGAATTGTGGTGAGA+TGG + chr3_1:78376772-78376791 None:intergenic 40.0%
AGAGGAAGTAGAGAGAATTG+TGG + chr3_1:78376780-78376799 None:intergenic 40.0%
ATCGATGTAGATAGAGACAG+AGG + chr3_1:78376798-78376817 None:intergenic 40.0%
AGCTCTTACTAGTTAGTTGC+TGG + chr3_1:78376823-78376842 None:intergenic 40.0%
! AGTGGCTAGTGAAACTGTTT+TGG - chr3_1:78376947-78376966 Msa0377750:intron 40.0%
TCGGTAATCCCTATCAAAAG+AGG + chr3_1:78377285-78377304 None:intergenic 40.0%
TTTGATAGGGATTACCGACA+TGG - chr3_1:78377287-78377306 Msa0377750:intron 40.0%
GAGAAAATGGATCTCCATGT+CGG + chr3_1:78377304-78377323 None:intergenic 40.0%
ATCATTGTCTTCCAACTCCT+TGG + chr3_1:78377406-78377425 None:intergenic 40.0%
TTCTTTGACGCTCTCATTTC+TGG - chr3_1:78377429-78377448 Msa0377750:CDS 40.0%
! GAGATTAAGGGTGCACAATA+TGG - chr3_1:78377691-78377710 Msa0377750:intron 40.0%
GGATGAAGAAGAAGAAGCTT+TGG - chr3_1:78377768-78377787 Msa0377750:CDS 40.0%
GTAAAGTAGCTAGCAGCATT+AGG - chr3_1:78377880-78377899 Msa0377750:intron 40.0%
CAGATTGTGGTATCTTTGGA+AGG - chr3_1:78378237-78378256 Msa0377750:intron 40.0%
GCCAAAGATGTAATGGTTGA+AGG + chr3_1:78378351-78378370 None:intergenic 40.0%
ATGGGTAGCCAAAGATGTAA+TGG + chr3_1:78378358-78378377 None:intergenic 40.0%
! GGATAATTCCTCTGAGAGAT+GGG + chr3_1:78378376-78378395 None:intergenic 40.0%
! TGGATAATTCCTCTGAGAGA+TGG + chr3_1:78378377-78378396 None:intergenic 40.0%
! GACTTTCTCCACTTTGTTTG+CGG - chr3_1:78378466-78378485 Msa0377750:five_prime_UTR 40.0%
CGTAGCTAAATTAAGGTGGT+AGG + chr3_1:78376666-78376685 None:intergenic 40.0%
ACTCCGTAGCTAAATTAAGG+TGG + chr3_1:78376670-78376689 None:intergenic 40.0%
CACACTCCGTAGCTAAATTA+AGG + chr3_1:78376673-78376692 None:intergenic 40.0%
TAGAGAGAATTGTGGTGAGA+TGG + chr3_1:78376772-78376791 None:intergenic 40.0%
AGAGGAAGTAGAGAGAATTG+TGG + chr3_1:78376780-78376799 None:intergenic 40.0%
ATCGATGTAGATAGAGACAG+AGG + chr3_1:78376798-78376817 None:intergenic 40.0%
AGCTCTTACTAGTTAGTTGC+TGG + chr3_1:78376823-78376842 None:intergenic 40.0%
! AGTGGCTAGTGAAACTGTTT+TGG - chr3_1:78376947-78376966 Msa0377750:intron 40.0%
TCGGTAATCCCTATCAAAAG+AGG + chr3_1:78377285-78377304 None:intergenic 40.0%
TTTGATAGGGATTACCGACA+TGG - chr3_1:78377287-78377306 Msa0377750:intron 40.0%
GAGAAAATGGATCTCCATGT+CGG + chr3_1:78377304-78377323 None:intergenic 40.0%
ATCATTGTCTTCCAACTCCT+TGG + chr3_1:78377406-78377425 None:intergenic 40.0%
TTCTTTGACGCTCTCATTTC+TGG - chr3_1:78377429-78377448 Msa0377750:CDS 40.0%
! GAGATTAAGGGTGCACAATA+TGG - chr3_1:78377691-78377710 Msa0377750:intron 40.0%
GGATGAAGAAGAAGAAGCTT+TGG - chr3_1:78377768-78377787 Msa0377750:CDS 40.0%
GTAAAGTAGCTAGCAGCATT+AGG - chr3_1:78377880-78377899 Msa0377750:intron 40.0%
CAGATTGTGGTATCTTTGGA+AGG - chr3_1:78378237-78378256 Msa0377750:intron 40.0%
GCCAAAGATGTAATGGTTGA+AGG + chr3_1:78378351-78378370 None:intergenic 40.0%
ATGGGTAGCCAAAGATGTAA+TGG + chr3_1:78378358-78378377 None:intergenic 40.0%
! GGATAATTCCTCTGAGAGAT+GGG + chr3_1:78378376-78378395 None:intergenic 40.0%
! TGGATAATTCCTCTGAGAGA+TGG + chr3_1:78378377-78378396 None:intergenic 40.0%
! GACTTTCTCCACTTTGTTTG+CGG - chr3_1:78378466-78378485 Msa0377750:five_prime_UTR 40.0%
ACCAAAACCCTTTCTCTCCT+TGG - chr3_1:78377005-78377024 Msa0377750:intron 45.0%
! ACCAAGGAGAGAAAGGGTTT+TGG + chr3_1:78377009-78377028 None:intergenic 45.0%
ACCATGACCAAGGAGAGAAA+GGG + chr3_1:78377015-78377034 None:intergenic 45.0%
TACCATGACCAAGGAGAGAA+AGG + chr3_1:78377016-78377035 None:intergenic 45.0%
GTAGCAACTTACCATGACCA+AGG + chr3_1:78377025-78377044 None:intergenic 45.0%
TGCAAGTGGCTTACTACTTC+AGG + chr3_1:78377363-78377382 None:intergenic 45.0%
TAGTAAGCCACTTGCAACAG+TGG - chr3_1:78377367-78377386 Msa0377750:CDS 45.0%
TACCTTTCCACTGTTGCAAG+TGG + chr3_1:78377377-78377396 None:intergenic 45.0%
AGTCTAATTGCCTTCTCCAC+AGG + chr3_1:78377658-78377677 None:intergenic 45.0%
! ATTTGTCATCGGCTGCCAAA+CGG + chr3_1:78377744-78377763 None:intergenic 45.0%
! AGCTACTTTACCTCAGCAGT+TGG + chr3_1:78377871-78377890 None:intergenic 45.0%
!! AATGGTATCTTGCTGGCTCA+AGG - chr3_1:78378316-78378335 Msa0377750:intron 45.0%
!! ATGGTATCTTGCTGGCTCAA+GGG - chr3_1:78378317-78378336 Msa0377750:intron 45.0%
GCAGAATTCCGCAAACAAAG+TGG + chr3_1:78378477-78378496 None:intergenic 45.0%
CATTGCACGTGTGGAATCTA+TGG + chr3_1:78378587-78378606 None:intergenic 45.0%
CATAGATTCCACACGTGCAA+TGG - chr3_1:78378585-78378604 Msa0377750:five_prime_UTR 45.0%
! ATAGTTTGCCATTGCACGTG+TGG + chr3_1:78378596-78378615 None:intergenic 45.0%
ACCAAAACCCTTTCTCTCCT+TGG - chr3_1:78377005-78377024 Msa0377750:intron 45.0%
! ACCAAGGAGAGAAAGGGTTT+TGG + chr3_1:78377009-78377028 None:intergenic 45.0%
ACCATGACCAAGGAGAGAAA+GGG + chr3_1:78377015-78377034 None:intergenic 45.0%
TACCATGACCAAGGAGAGAA+AGG + chr3_1:78377016-78377035 None:intergenic 45.0%
GTAGCAACTTACCATGACCA+AGG + chr3_1:78377025-78377044 None:intergenic 45.0%
TGCAAGTGGCTTACTACTTC+AGG + chr3_1:78377363-78377382 None:intergenic 45.0%
TAGTAAGCCACTTGCAACAG+TGG - chr3_1:78377367-78377386 Msa0377750:CDS 45.0%
TACCTTTCCACTGTTGCAAG+TGG + chr3_1:78377377-78377396 None:intergenic 45.0%
AGTCTAATTGCCTTCTCCAC+AGG + chr3_1:78377658-78377677 None:intergenic 45.0%
! ATTTGTCATCGGCTGCCAAA+CGG + chr3_1:78377744-78377763 None:intergenic 45.0%
! AGCTACTTTACCTCAGCAGT+TGG + chr3_1:78377871-78377890 None:intergenic 45.0%
!! AATGGTATCTTGCTGGCTCA+AGG - chr3_1:78378316-78378335 Msa0377750:intron 45.0%
!! ATGGTATCTTGCTGGCTCAA+GGG - chr3_1:78378317-78378336 Msa0377750:intron 45.0%
GCAGAATTCCGCAAACAAAG+TGG + chr3_1:78378477-78378496 None:intergenic 45.0%
CATTGCACGTGTGGAATCTA+TGG + chr3_1:78378587-78378606 None:intergenic 45.0%
CATAGATTCCACACGTGCAA+TGG - chr3_1:78378585-78378604 Msa0377750:five_prime_UTR 45.0%
! ATAGTTTGCCATTGCACGTG+TGG + chr3_1:78378596-78378615 None:intergenic 45.0%
!! TTTAAAAAATAAATAAATTG+AGG - chr3_1:78378021-78378040 Msa0377750:intron 5.0%
!! TTTAAAAAATAAATAAATTG+AGG - chr3_1:78378021-78378040 Msa0377750:intron 5.0%
ACCCTTTCTCTCCTTGGTCA+TGG - chr3_1:78377011-78377030 Msa0377750:intron 50.0%
AGCCACTTGCAACAGTGGAA+AGG - chr3_1:78377372-78377391 Msa0377750:CDS 50.0%
TTGTCTTCCAACTCCTTGGC+AGG + chr3_1:78377402-78377421 None:intergenic 50.0%
GTGAATATGTGCAGCTCCTG+TGG - chr3_1:78377639-78377658 Msa0377750:intron 50.0%
TTCAGATTGAGACCGCTGTG+AGG - chr3_1:78377716-78377735 Msa0377750:intron 50.0%
TCAGATTGAGACCGCTGTGA+GGG - chr3_1:78377717-78377736 Msa0377750:intron 50.0%
GGCAGCCGATGACAAATATG+AGG - chr3_1:78377747-78377766 Msa0377750:CDS 50.0%
! CAGCAGTTGGAGAGTTTGCT+GGG + chr3_1:78377858-78377877 None:intergenic 50.0%
TCAGCAGTTGGAGAGTTTGC+TGG + chr3_1:78377859-78377878 None:intergenic 50.0%
AGCAAACTCTCCAACTGCTG+AGG - chr3_1:78377858-78377877 Msa0377750:CDS 50.0%
TTTGGCTACCCATCTCTCAG+AGG - chr3_1:78378365-78378384 Msa0377750:five_prime_UTR 50.0%
ACCCTTTCTCTCCTTGGTCA+TGG - chr3_1:78377011-78377030 Msa0377750:intron 50.0%
AGCCACTTGCAACAGTGGAA+AGG - chr3_1:78377372-78377391 Msa0377750:CDS 50.0%
TTGTCTTCCAACTCCTTGGC+AGG + chr3_1:78377402-78377421 None:intergenic 50.0%
GTGAATATGTGCAGCTCCTG+TGG - chr3_1:78377639-78377658 Msa0377750:intron 50.0%
TTCAGATTGAGACCGCTGTG+AGG - chr3_1:78377716-78377735 Msa0377750:intron 50.0%
TCAGATTGAGACCGCTGTGA+GGG - chr3_1:78377717-78377736 Msa0377750:intron 50.0%
GGCAGCCGATGACAAATATG+AGG - chr3_1:78377747-78377766 Msa0377750:CDS 50.0%
! CAGCAGTTGGAGAGTTTGCT+GGG + chr3_1:78377858-78377877 None:intergenic 50.0%
TCAGCAGTTGGAGAGTTTGC+TGG + chr3_1:78377859-78377878 None:intergenic 50.0%
AGCAAACTCTCCAACTGCTG+AGG - chr3_1:78377858-78377877 Msa0377750:CDS 50.0%
TTTGGCTACCCATCTCTCAG+AGG - chr3_1:78378365-78378384 Msa0377750:five_prime_UTR 50.0%
GTGGAAAGGTAAGCCTGCCA+AGG - chr3_1:78377386-78377405 Msa0377750:CDS 55.0%
AGGTAAGCCTGCCAAGGAGT+TGG - chr3_1:78377392-78377411 Msa0377750:CDS 55.0%
ATGTGCAGCTCCTGTGGAGA+AGG - chr3_1:78377645-78377664 Msa0377750:intron 55.0%
GTGGAAAGGTAAGCCTGCCA+AGG - chr3_1:78377386-78377405 Msa0377750:CDS 55.0%
AGGTAAGCCTGCCAAGGAGT+TGG - chr3_1:78377392-78377411 Msa0377750:CDS 55.0%
ATGTGCAGCTCCTGTGGAGA+AGG - chr3_1:78377645-78377664 Msa0377750:intron 55.0%
TGCCAAACGGTCCCTCACAG+CGG + chr3_1:78377731-78377750 None:intergenic 60.0%
TGCCAAACGGTCCCTCACAG+CGG + chr3_1:78377731-78377750 None:intergenic 60.0%
GACCGCTGTGAGGGACCGTT+TGG - chr3_1:78377726-78377745 Msa0377750:intron 65.0%
GACCGCTGTGAGGGACCGTT+TGG - chr3_1:78377726-78377745 Msa0377750:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 78376549 78378634 78376549 ID=Msa0377750;Name=Msa0377750
chr3_1 mRNA 78376549 78378634 78376549 ID=Msa0377750-mRNA-1;Parent=Msa0377750;Name=Msa0377750-mRNA-1;_AED=0.03;_eAED=0.03;_QI=284|1|1|1|0.75|0.8|5|233|209
chr3_1 exon 78376549 78376943 78376549 ID=Msa0377750-mRNA-1:exon:18864;Parent=Msa0377750-mRNA-1
chr3_1 exon 78377304 78377530 78377304 ID=Msa0377750-mRNA-1:exon:18863;Parent=Msa0377750-mRNA-1
chr3_1 exon 78377733 78377888 78377733 ID=Msa0377750-mRNA-1:exon:18862;Parent=Msa0377750-mRNA-1
chr3_1 exon 78378151 78378234 78378151 ID=Msa0377750-mRNA-1:exon:18861;Parent=Msa0377750-mRNA-1
chr3_1 exon 78378350 78378634 78378350 ID=Msa0377750-mRNA-1:exon:18860;Parent=Msa0377750-mRNA-1
chr3_1 five_prime_UTR 78378351 78378634 78378351 ID=Msa0377750-mRNA-1:five_prime_utr;Parent=Msa0377750-mRNA-1
chr3_1 CDS 78378350 78378350 78378350 ID=Msa0377750-mRNA-1:cds;Parent=Msa0377750-mRNA-1
chr3_1 CDS 78378151 78378234 78378151 ID=Msa0377750-mRNA-1:cds;Parent=Msa0377750-mRNA-1
chr3_1 CDS 78377733 78377888 78377733 ID=Msa0377750-mRNA-1:cds;Parent=Msa0377750-mRNA-1
chr3_1 CDS 78377304 78377530 78377304 ID=Msa0377750-mRNA-1:cds;Parent=Msa0377750-mRNA-1
chr3_1 CDS 78376782 78376943 78376782 ID=Msa0377750-mRNA-1:cds;Parent=Msa0377750-mRNA-1
chr3_1 three_prime_UTR 78376549 78376781 78376549 ID=Msa0377750-mRNA-1:three_prime_utr;Parent=Msa0377750-mRNA-1
Gene Sequence

>Msa0377750

ATGGCTAGTGAAACTGTTTTGGTCGATGAAATCTCTTACCCTTCAAAGATCACTACTACCAAAACCCTTTCTCTCCTTGGTCATGGGATTACCGACATGGAGATCCATTTTCTCCAAGTGAAATTCTATTCAATTGGGGTTTATTTGGAACCTGAAGTAGTAAGCCACTTGCAACAGTGGAAAGGTAAGCCTGCCAAGGAGTTGGAAGACAATGATGACTTCTTTGACGCTCTCATTTCTGCTCCTGTGGAGAAGGCAATTAGACTTGTTGTGATTAAAGAGATTAAGGGTGCACAATATGGTGTTCAGATTGAGACCGCTGTGAGGGACCGTTTGGCAGCCGATGACAAATATGAGGATGAAGAAGAAGAAGCTTTGGAAAAAGTAATTGAATTTTTCCAGTCTAAGTACTTCAAGAAACACTCAGTCATCACATATCATTTCCCAGCAAACTCTCCAACTGCTGAGATTGTGGTATCTTTGGAAGGGAAAGAAGACTCAAAGTATGTAATAGAGAATGGCAATGTAGTAGAGACTATTAAGAAATGGTATCTTGCTGGCTCAAGGGCTATATCTCCTTCAACCATTACATCTTTGGCTACCCATCTCTCAGAGGAATTATCCAAGTGA
Protein sequence

>Msa0377750

MASETVLVDEISYPSKITTTKTLSLLGHGITDMEIHFLQVKFYSIGVYLEPEVVSHLQQWKGKPAKELEDNDDFFDALISAPVEKAIRLVVIKEIKGAQYGVQIETAVRDRLAADDKYEDEEEEALEKVIEFFQSKYFKKHSVITYHFPANSPTAEIVVSLEGKEDSKYVIENGNVVETIKKWYLAGSRAISPSTITSLATHLSEELSK*