AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0383160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383160 MtrunA17_Chr3g0138541 87.658 316 4 2 1 281 1 316 0.0 526
Msa0383160 MtrunA17_Chr3g0138531 80.782 307 33 3 1 281 1 307 9.22e-94 279
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383160 AT2G25670.2 56.426 319 100 8 1 281 1 318 1.29e-37 135
Msa0383160 AT2G25670.1 56.426 319 100 8 1 281 1 318 1.29e-37 135
Msa0383160 AT4G32610.1 56.508 315 103 7 1 281 1 315 6.75e-32 120

Find 90 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTCTTCTTGGCATTCT+TGG 0.196635 3_1:+85932433 None:intergenic
AATCGGAATCGCAGGCGTTT+TGG 0.237603 3_1:-85934753 Msa0383160:CDS
CGATTCCGATTTGCTCGATT+TGG 0.247149 3_1:+85934771 None:intergenic
TTCACAGCATGCTCTGGTTC+TGG 0.268354 3_1:+85933033 None:intergenic
TCGAATAAATTGTTGGCTTT+AGG 0.283201 3_1:-85932055 Msa0383160:three_prime_UTR
CTTCTTGGCATTCTTGGACT+CGG 0.319126 3_1:+85932439 None:intergenic
GCTTGCTGCAGCAAAGAAAA+AGG 0.330235 3_1:-85932174 Msa0383160:CDS
ATTAAATTAATTCATACAAC+AGG 0.346248 3_1:-85935091 Msa0383160:five_prime_UTR
ATGCTCCGGTTTGTCCTCAC+TGG 0.348989 3_1:+85934630 None:intergenic
GCACTGGTTGTTGGTTATAA+TGG 0.350920 3_1:+85932144 None:intergenic
TTCTTGGCATTCTTGGACTC+GGG 0.367598 3_1:+85932440 None:intergenic
AGACAATATATCAAGTCTAT+TGG 0.367743 3_1:+85932010 None:intergenic
GAAGAAATCAAGTAAAGAAA+TGG 0.370591 3_1:-85932246 Msa0383160:CDS
AGTTGTCGCTCTGCCTCCTT+TGG 0.375124 3_1:+85932967 None:intergenic
GGAAAGTGTTGTCGCTTCTA+TGG 0.376593 3_1:-85931827 Msa0383160:three_prime_UTR
TATAATGTCGAATAAATTGT+TGG 0.377100 3_1:-85932062 Msa0383160:three_prime_UTR
GACAAGGACAAGAAGAATAA+AGG 0.383986 3_1:-85934799 Msa0383160:CDS
AATTTAATCAATTTGAGATC+TGG 0.398927 3_1:+85935135 None:intergenic
CTGTAATTAAACACTAAAAT+CGG 0.399136 3_1:-85934951 Msa0383160:five_prime_UTR
AGAGCGACAACTCTCCAAAA+AGG 0.404514 3_1:-85932956 Msa0383160:CDS
GTTTGTCCTCACTGGGTTGA+GGG 0.409258 3_1:+85934638 None:intergenic
GGTTTGTCCTCACTGGGTTG+AGG 0.417767 3_1:+85934637 None:intergenic
TCAGTCTTCACAGCATGCTC+TGG 0.423791 3_1:+85933027 None:intergenic
ATTAGATTCTTTCACCTCCT+TGG 0.431741 3_1:+85932394 None:intergenic
GGTGTTGAAGGGGATGTTGA+TGG 0.444637 3_1:-85932482 Msa0383160:CDS
CAAATCGAGCAAATCGGAAT+CGG 0.447655 3_1:-85934770 Msa0383160:CDS
AAATTGTTGGCTTTAGGCTC+CGG 0.456776 3_1:-85932049 Msa0383160:three_prime_UTR
CGCCGTTGAATGCAACCTCA+TGG 0.467633 3_1:-85934723 Msa0383160:CDS
CAGGATAAGAAAGGTGTTGA+AGG 0.477670 3_1:-85932494 Msa0383160:CDS
TCTGCCTCCTTTGGCACCAC+AGG 0.481597 3_1:+85932976 None:intergenic
TGGTGGAAGCCGAAGGGATG+AGG 0.482889 3_1:-85934890 Msa0383160:CDS
CTGCTCAAGAAGCTGCTGCT+AGG 0.485825 3_1:-85932205 Msa0383160:CDS
TCAGCGTGCTTCTTAACCTC+AGG 0.486662 3_1:+85933003 None:intergenic
TGCTCCGGTTTGTCCTCACT+GGG 0.505009 3_1:+85934631 None:intergenic
TTCTTCCAAATCGAGCAAAT+CGG 0.505042 3_1:-85934776 Msa0383160:CDS
AGATGACGGTGATGAAGAGG+AGG 0.506605 3_1:-85933073 Msa0383160:CDS
TCAACCCAGTGAGGACAAAC+CGG 0.510598 3_1:-85934635 Msa0383160:CDS
GCTCTCCTTTACATCAGTAC+AGG 0.513204 3_1:-85932107 Msa0383160:three_prime_UTR
ACTCAGAAAGAAAGTAATGA+TGG 0.515814 3_1:-85932877 Msa0383160:intron
GTAAACTTCAACTACTCTCA+TGG 0.517149 3_1:+85931853 None:intergenic
GAACTATGTTATTGTACACC+GGG 0.518020 3_1:-85931906 Msa0383160:three_prime_UTR
AGAGTAGTTGAAGTTTACTC+TGG 0.522458 3_1:-85931848 Msa0383160:three_prime_UTR
AAATTCCTGTACTGATGTAA+AGG 0.523777 3_1:+85932102 None:intergenic
GCCCATGAGGTTGCATTCAA+CGG 0.525909 3_1:+85934721 None:intergenic
AAAGAAGGATAAAGCTTCCA+AGG 0.528000 3_1:-85932411 Msa0383160:CDS
AATTTGTGATTAACAATGGT+TGG 0.528702 3_1:-85934913 Msa0383160:exon
CAAATCGGAATCGGAATCGC+AGG 0.529120 3_1:-85934761 Msa0383160:CDS
TGTATTAGCAGGAGTACCTC+AGG 0.534708 3_1:-85932513 Msa0383160:intron
TGATTAACAATGGTTGGTGG+TGG 0.548951 3_1:-85934907 Msa0383160:exon
GCCGTTGAATGCAACCTCAT+GGG 0.549582 3_1:-85934722 Msa0383160:CDS
GGTGGAAGCCGAAGGGATGA+GGG 0.549738 3_1:-85934889 Msa0383160:CDS
TTTAGATGACGGTGATGAAG+AGG 0.551847 3_1:-85933076 Msa0383160:CDS
GAGCAAACCTCAAAATGCTC+CGG 0.553859 3_1:+85934616 None:intergenic
CCGAAGGGATGAGGGTTCGT+TGG 0.558950 3_1:-85934881 Msa0383160:CDS
GAGGTTGCATTCAACGGCGC+CGG 0.563482 3_1:+85934727 None:intergenic
GGAGTACCTCAGGATAAGAA+AGG 0.568562 3_1:-85932503 Msa0383160:CDS
TGAACTATGTTATTGTACAC+CGG 0.569386 3_1:-85931907 Msa0383160:three_prime_UTR
AAAGGAACTTGAAGAGCTTG+AGG 0.570478 3_1:-85932923 Msa0383160:CDS
AAGCTGCTGCTAGGAATGCA+AGG 0.570860 3_1:-85932196 Msa0383160:CDS
TACTGATGTAAAGGAGAGCA+AGG 0.571309 3_1:+85932111 None:intergenic
GGTGAATTTGTGATTAACAA+TGG 0.572570 3_1:-85934917 Msa0383160:exon
TCAACACCTTTCTTATCCTG+AGG 0.575733 3_1:+85932497 None:intergenic
TGAAGTTCCTGTGGTGCCAA+AGG 0.576188 3_1:-85932983 Msa0383160:CDS
AGAAAATTGAGGCGGTGCGG+TGG 0.576432 3_1:+85934663 None:intergenic
AGGATAAGAAAGGTGTTGAA+GGG 0.576801 3_1:-85932493 Msa0383160:CDS
CCAACGAACCCTCATCCCTT+CGG 0.577226 3_1:+85934881 None:intergenic
TTGTGATTAACAATGGTTGG+TGG 0.584390 3_1:-85934910 Msa0383160:exon
TAAACTTCAACTACTCTCAT+GGG 0.586268 3_1:+85931854 None:intergenic
TTAACAGGATACTGAGAGCG+AGG 0.605757 3_1:-85933106 Msa0383160:intron
TGGTTGGTGGTGGAAGCCGA+AGG 0.610857 3_1:-85934897 Msa0383160:CDS
GGTTGAGGGTCAGAAAATTG+AGG 0.611108 3_1:+85934652 None:intergenic
TGGAATACGACTAGAGTCAC+CGG 0.614862 3_1:+85932030 None:intergenic
AGTTCCTGTGGTGCCAAAGG+AGG 0.616505 3_1:-85932980 Msa0383160:CDS
GGATAAGAAAGGTGTTGAAG+GGG 0.623648 3_1:-85932492 Msa0383160:CDS
GTCAGAAAATTGAGGCGGTG+CGG 0.648535 3_1:+85934660 None:intergenic
TTCTGACCCTCAACCCAGTG+AGG 0.661638 3_1:-85934644 Msa0383160:CDS
GTCATCAACATCAGCCCATG+AGG 0.663150 3_1:+85934708 None:intergenic
GGAGATAAACAAAACAGAGG+AGG 0.663490 3_1:+85935194 None:intergenic
GAAGCACGCTGAAGTTCCTG+TGG 0.664946 3_1:-85932992 Msa0383160:CDS
TGCTGTGAAGACTGAACCTG+AGG 0.675373 3_1:-85933019 Msa0383160:CDS
GGTTGGTGGTGGAAGCCGAA+GGG 0.685517 3_1:-85934896 Msa0383160:CDS
TTAAATTAATTCATACAACA+GGG 0.685908 3_1:-85935090 Msa0383160:five_prime_UTR
AACTATGTTATTGTACACCG+GGG 0.694693 3_1:-85931905 Msa0383160:three_prime_UTR
GAAGAAGAAGAAATCAGACA+AGG 0.694695 3_1:-85934815 Msa0383160:CDS
GAACCGACAATTTAGCGGCG+AGG 0.707051 3_1:-85935003 Msa0383160:intron
TGAGGGTCAGAAAATTGAGG+CGG 0.709234 3_1:+85934655 None:intergenic
CATACACGACATAAGAACCC+CGG 0.711851 3_1:+85931888 None:intergenic
TTTGGAGATAAACAAAACAG+AGG 0.720148 3_1:+85935191 None:intergenic
GAAGGATAAAGCTTCCAAGG+AGG 0.726948 3_1:-85932408 Msa0383160:intron
ATTATAACCAACAACCAGTG+CGG 0.740729 3_1:-85932142 Msa0383160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATTTAAATATAAGAAAAT+AGG + chr3_1:85933754-85933773 None:intergenic 10.0%
!! ATTAAATTAATTCATACAAC+AGG - chr3_1:85931906-85931925 Msa0383160:three_prime_UTR 15.0%
!! TTAAATTAATTCATACAACA+GGG - chr3_1:85931907-85931926 Msa0383160:three_prime_UTR 15.0%
!!! ATTTTAAATCTGCTAATATA+TGG + chr3_1:85933285-85933304 None:intergenic 15.0%
!!! AATTTAATCAATTTGAGATC+TGG + chr3_1:85931865-85931884 None:intergenic 20.0%
!! CTGTAATTAAACACTAAAAT+CGG - chr3_1:85932046-85932065 Msa0383160:three_prime_UTR 20.0%
!! TGTAAAATAGAAGAAATGTT+TGG + chr3_1:85933109-85933128 None:intergenic 20.0%
!! GTCTTATGATATTTATGTTT+AGG - chr3_1:85933421-85933440 Msa0383160:intron 20.0%
!!! ATTTTCTGTAATTTGGTATT+TGG - chr3_1:85933680-85933699 Msa0383160:intron 20.0%
!! TATAATGTCGAATAAATTGT+TGG - chr3_1:85934935-85934954 Msa0383160:five_prime_UTR 20.0%
!!! TTTTATAGACTATGACATTT+TGG - chr3_1:85935040-85935059 Msa0383160:five_prime_UTR 20.0%
! AGACAGAAAAATAGAGATTA+GGG + chr3_1:85931832-85931851 None:intergenic 25.0%
! AAGACAGAAAAATAGAGATT+AGG + chr3_1:85931833-85931852 None:intergenic 25.0%
! AATTTGTGATTAACAATGGT+TGG - chr3_1:85932084-85932103 Msa0383160:three_prime_UTR 25.0%
! TTTATACCTTTCTGTTTGTT+TGG - chr3_1:85932436-85932455 Msa0383160:CDS 25.0%
!! TTCAATTGCTTTATTGTGTT+AGG - chr3_1:85932460-85932479 Msa0383160:CDS 25.0%
! TTGACATATTCTTTGTGTAT+TGG - chr3_1:85932540-85932559 Msa0383160:intron 25.0%
! AACGTTACATCATATCATAA+TGG - chr3_1:85932754-85932773 Msa0383160:intron 25.0%
! ACGTTACATCATATCATAAT+GGG - chr3_1:85932755-85932774 Msa0383160:intron 25.0%
!!! ATGGGTTTTATGACAAAAAT+AGG + chr3_1:85933169-85933188 None:intergenic 25.0%
! TTTGTCATAAAACCCATATT+TGG - chr3_1:85933172-85933191 Msa0383160:intron 25.0%
!! CAGATTTAAAATCCTTTGAA+TGG - chr3_1:85933293-85933312 Msa0383160:intron 25.0%
! AAGTTACTATTACCATTCAA+AGG + chr3_1:85933308-85933327 None:intergenic 25.0%
! TTGAATGGTAATAGTAACTT+AGG - chr3_1:85933308-85933327 Msa0383160:intron 25.0%
! TAATAGCAGAATTGTGTAAT+AGG - chr3_1:85933447-85933466 Msa0383160:intron 25.0%
!! TTGCTGTATTTTCTGTAATT+TGG - chr3_1:85933673-85933692 Msa0383160:intron 25.0%
! ACACTAATTTGAACAGAAAT+AGG + chr3_1:85933715-85933734 None:intergenic 25.0%
! CTCTATATTACATTATTTGC+AGG - chr3_1:85934180-85934199 Msa0383160:intron 25.0%
! TTAAATAGAAAAACCAGCTA+GGG + chr3_1:85934341-85934360 None:intergenic 25.0%
! AATTGCAAAATTGTATTAGC+AGG - chr3_1:85934473-85934492 Msa0383160:intron 25.0%
! GAAGAAATCAAGTAAAGAAA+TGG - chr3_1:85934751-85934770 Msa0383160:CDS 25.0%
! AGACAATATATCAAGTCTAT+TGG + chr3_1:85934990-85935009 None:intergenic 25.0%
!!! TTGTCTTATGCTTTTTTTTC+TGG - chr3_1:85935004-85935023 Msa0383160:intron 25.0%
!!! TGTCTTATGCTTTTTTTTCT+GGG - chr3_1:85935005-85935024 Msa0383160:intron 25.0%
TTTGGAGATAAACAAAACAG+AGG + chr3_1:85931809-85931828 None:intergenic 30.0%
GAAAAATAGAGATTAGGGTT+TGG + chr3_1:85931827-85931846 None:intergenic 30.0%
! TTTTTGAACCGACAATTTAG+CGG - chr3_1:85931989-85932008 Msa0383160:three_prime_UTR 30.0%
!! ACACTAAAATCGGTTTTTGT+TGG - chr3_1:85932056-85932075 Msa0383160:three_prime_UTR 30.0%
GGTGAATTTGTGATTAACAA+TGG - chr3_1:85932080-85932099 Msa0383160:three_prime_UTR 30.0%
TTGAATCCAAACAAACAGAA+AGG + chr3_1:85932445-85932464 None:intergenic 30.0%
CTTTATTGTGTTAGGAAACA+TGG - chr3_1:85932468-85932487 Msa0383160:CDS 30.0%
!!! TATAGCTGTCCTTTTTTTGT+TGG - chr3_1:85932577-85932596 Msa0383160:intron 30.0%
ACTTAATTTCATAAACTCCC+AGG + chr3_1:85932731-85932750 None:intergenic 30.0%
GAAATTCAAAAAGTTACGAC+AGG - chr3_1:85932777-85932796 Msa0383160:intron 30.0%
CTGTCGTAACTATGTAATAA+CGG + chr3_1:85932840-85932859 None:intergenic 30.0%
GGAAAATAGGAAATAAATGG+AGG + chr3_1:85932920-85932939 None:intergenic 30.0%
GTGGGAAAATAGGAAATAAA+TGG + chr3_1:85932923-85932942 None:intergenic 30.0%
TGTATACACTTACCATACAA+GGG + chr3_1:85932950-85932969 None:intergenic 30.0%
TTGTATACACTTACCATACA+AGG + chr3_1:85932951-85932970 None:intergenic 30.0%
! GTTGCTTTGGATTTATTTCA+TGG + chr3_1:85933082-85933101 None:intergenic 30.0%
TTACTAGCATCACCAAATAT+GGG + chr3_1:85933187-85933206 None:intergenic 30.0%
ATTACTAGCATCACCAAATA+TGG + chr3_1:85933188-85933207 None:intergenic 30.0%
GAAAGGAATAATAGTGATTG+TGG - chr3_1:85933531-85933550 Msa0383160:intron 30.0%
! TGTGTTACTGTAATTTCTGT+AGG - chr3_1:85933616-85933635 Msa0383160:intron 30.0%
AAACTGAAACGTATCAAATC+TGG + chr3_1:85933872-85933891 None:intergenic 30.0%
!! TGATACGTTTCAGTTTTAAC+AGG - chr3_1:85933876-85933895 Msa0383160:intron 30.0%
!! GAGGAAGATATTTTAGATGA+CGG - chr3_1:85933910-85933929 Msa0383160:intron 30.0%
! CTTTTTCTTCCTTTCCTTTT+TGG + chr3_1:85934058-85934077 None:intergenic 30.0%
CAAAAAGGAAAGGAAGAAAA+AGG - chr3_1:85934056-85934075 Msa0383160:intron 30.0%
ACTCAGAAAGAAAGTAATGA+TGG - chr3_1:85934120-85934139 Msa0383160:intron 30.0%
!!! TCTACACTATTGGCATTTTT+TGG - chr3_1:85934262-85934281 Msa0383160:intron 30.0%
GTTAAATAGAAAAACCAGCT+AGG + chr3_1:85934342-85934361 None:intergenic 30.0%
!!! AGCTGGTTTTTCTATTTAAC+TGG - chr3_1:85934342-85934361 Msa0383160:intron 30.0%
!!! GCTGGTTTTTCTATTTAACT+GGG - chr3_1:85934343-85934362 Msa0383160:intron 30.0%
ATATGTAGTACAATCACTTG+AGG - chr3_1:85934425-85934444 Msa0383160:intron 30.0%
! ATCCTTCTTTTTCTTCTTCT+TGG + chr3_1:85934576-85934595 None:intergenic 30.0%
! AAATTCCTGTACTGATGTAA+AGG + chr3_1:85934898-85934917 None:intergenic 30.0%
!! TCGAATAAATTGTTGGCTTT+AGG - chr3_1:85934942-85934961 Msa0383160:five_prime_UTR 30.0%
TGAACTATGTTATTGTACAC+CGG - chr3_1:85935090-85935109 Msa0383160:five_prime_UTR 30.0%
TAAACTTCAACTACTCTCAT+GGG + chr3_1:85935146-85935165 None:intergenic 30.0%
!! CTAAAATCGGTTTTTGTTGG+TGG - chr3_1:85932059-85932078 Msa0383160:three_prime_UTR 35.0%
TTGTGATTAACAATGGTTGG+TGG - chr3_1:85932087-85932106 Msa0383160:three_prime_UTR 35.0%
GAAGAAGAAGAAATCAGACA+AGG - chr3_1:85932182-85932201 Msa0383160:CDS 35.0%
GACAAGGACAAGAAGAATAA+AGG - chr3_1:85932198-85932217 Msa0383160:CDS 35.0%
TTCTTCCAAATCGAGCAAAT+CGG - chr3_1:85932221-85932240 Msa0383160:CDS 35.0%
TATTGTGTTAGGAAACATGG+TGG - chr3_1:85932471-85932490 Msa0383160:CDS 35.0%
TCTGCAGTACCAACAAAAAA+AGG + chr3_1:85932589-85932608 None:intergenic 35.0%
CTGTTTGTATAGACTAACCT+GGG - chr3_1:85932711-85932730 Msa0383160:intron 35.0%
CGTTATTACATAGTTACGAC+AGG - chr3_1:85932838-85932857 Msa0383160:intron 35.0%
! TGCATTTTGTAGCTTTTCGA+TGG + chr3_1:85932868-85932887 None:intergenic 35.0%
AAACTGTCGTCAAATTTCGT+CGG - chr3_1:85932969-85932988 Msa0383160:CDS 35.0%
AATGTTTGGATGAGTTGCTT+TGG + chr3_1:85933095-85933114 None:intergenic 35.0%
!! GTAGTCTTGTAAAGAATAGC+TGG - chr3_1:85933226-85933245 Msa0383160:intron 35.0%
! TCTGTCACATGATTTTTGTG+TGG - chr3_1:85933335-85933354 Msa0383160:intron 35.0%
! CTGTCACATGATTTTTGTGT+GGG - chr3_1:85933336-85933355 Msa0383160:intron 35.0%
TGTGGGATATTTGTAAGTGA+TGG - chr3_1:85933353-85933372 Msa0383160:intron 35.0%
! TATTTGTAAGTGATGGAACC+AGG - chr3_1:85933360-85933379 Msa0383160:intron 35.0%
GCCATTGAACTTCCTAATAA+CGG + chr3_1:85933476-85933495 None:intergenic 35.0%
AAGTTCAATGGCAGTTATGT+TGG - chr3_1:85933484-85933503 Msa0383160:intron 35.0%
! GTGGTTGTTATTCTGTATGT+TGG - chr3_1:85933550-85933569 Msa0383160:intron 35.0%
GTTACTGTAATTTCTGTAGG+AGG - chr3_1:85933619-85933638 Msa0383160:intron 35.0%
TAGGAGGATAACTACTATGT+GGG - chr3_1:85933635-85933654 Msa0383160:intron 35.0%
CGTGAGTTCATGTTATTGTT+TGG - chr3_1:85934220-85934239 Msa0383160:intron 35.0%
!!! CACTATTGGCATTTTTTGGT+TGG - chr3_1:85934266-85934285 Msa0383160:intron 35.0%
!! AGGATAAGAAAGGTGTTGAA+GGG - chr3_1:85934504-85934523 Msa0383160:intron 35.0%
TTTCTTCTTCTTGGCATTCT+TGG + chr3_1:85934567-85934586 None:intergenic 35.0%
TGCCAAGAAGAAGAAAAAGA+AGG - chr3_1:85934571-85934590 Msa0383160:intron 35.0%
AAAGAAGGATAAAGCTTCCA+AGG - chr3_1:85934586-85934605 Msa0383160:intron 35.0%
ATTAGATTCTTTCACCTCCT+TGG + chr3_1:85934606-85934625 None:intergenic 35.0%
AAGGAGGTGAAAGAATCTAA+TGG - chr3_1:85934605-85934624 Msa0383160:intron 35.0%
!! TTGTTTGTTTCTGAGCTGTT+GGG + chr3_1:85934635-85934654 None:intergenic 35.0%
!! ATTGTTTGTTTCTGAGCTGT+TGG + chr3_1:85934636-85934655 None:intergenic 35.0%
AACAGCTCAGAAACAAACAA+TGG - chr3_1:85934635-85934654 Msa0383160:CDS 35.0%
ACAGCTCAGAAACAAACAAT+GGG - chr3_1:85934636-85934655 Msa0383160:CDS 35.0%
AACAAACAATGGGACTGATA+TGG - chr3_1:85934646-85934665 Msa0383160:CDS 35.0%
ATTATAACCAACAACCAGTG+CGG - chr3_1:85934855-85934874 Msa0383160:CDS 35.0%
GAACTATGTTATTGTACACC+GGG - chr3_1:85935091-85935110 Msa0383160:five_prime_UTR 35.0%
AACTATGTTATTGTACACCG+GGG - chr3_1:85935092-85935111 Msa0383160:five_prime_UTR 35.0%
GTAAACTTCAACTACTCTCA+TGG + chr3_1:85935147-85935166 None:intergenic 35.0%
AGAGTAGTTGAAGTTTACTC+TGG - chr3_1:85935149-85935168 Msa0383160:five_prime_UTR 35.0%
GGAGATAAACAAAACAGAGG+AGG + chr3_1:85931806-85931825 None:intergenic 40.0%
!! TGATTAACAATGGTTGGTGG+TGG - chr3_1:85932090-85932109 Msa0383160:three_prime_UTR 40.0%
CAAATCGAGCAAATCGGAAT+CGG - chr3_1:85932227-85932246 Msa0383160:CDS 40.0%
! TTAGGAAACATGGTGGAATC+AGG - chr3_1:85932478-85932497 Msa0383160:CDS 40.0%
TGGTGGAATCAGGATATTAG+TGG - chr3_1:85932488-85932507 Msa0383160:CDS 40.0%
GCTGTTTGTATAGACTAACC+TGG - chr3_1:85932710-85932729 Msa0383160:intron 40.0%
ATACACTTACCATACAAGGG+TGG + chr3_1:85932947-85932966 None:intergenic 40.0%
TATGATAGAGCCATGCTATG+CGG + chr3_1:85933007-85933026 None:intergenic 40.0%
TGTAATAGGCAGCCGTTATT+AGG - chr3_1:85933461-85933480 Msa0383160:intron 40.0%
GCCGTTATTAGGAAGTTCAA+TGG - chr3_1:85933472-85933491 Msa0383160:intron 40.0%
GTAGGAGGATAACTACTATG+TGG - chr3_1:85933634-85933653 Msa0383160:intron 40.0%
GTGATTTACCTCCGTTTGTT+TGG - chr3_1:85933805-85933824 Msa0383160:intron 40.0%
! TTTAGATGACGGTGATGAAG+AGG - chr3_1:85933921-85933940 Msa0383160:intron 40.0%
GACAACTCTCCAAAAAGGAA+AGG - chr3_1:85934046-85934065 Msa0383160:intron 40.0%
AAAGGAACTTGAAGAGCTTG+AGG - chr3_1:85934074-85934093 Msa0383160:intron 40.0%
!! GAGGCTCTTTTAGCTGATTT+TGG - chr3_1:85934093-85934112 Msa0383160:intron 40.0%
GTACCTTTGCTCTACACTAT+TGG - chr3_1:85934252-85934271 Msa0383160:intron 40.0%
ATGCCAATAGTGTAGAGCAA+AGG + chr3_1:85934258-85934277 None:intergenic 40.0%
GTAAATCTGTGAGCAAAAGC+AGG + chr3_1:85934299-85934318 None:intergenic 40.0%
TCAACACCTTTCTTATCCTG+AGG + chr3_1:85934503-85934522 None:intergenic 40.0%
!! CAGGATAAGAAAGGTGTTGA+AGG - chr3_1:85934503-85934522 Msa0383160:intron 40.0%
!! GGATAAGAAAGGTGTTGAAG+GGG - chr3_1:85934505-85934524 Msa0383160:intron 40.0%
! AAGATCCTCCTCCGAATTTT+CGG + chr3_1:85934681-85934700 None:intergenic 40.0%
!! GCACTGGTTGTTGGTTATAA+TGG + chr3_1:85934856-85934875 None:intergenic 40.0%
TACTGATGTAAAGGAGAGCA+AGG + chr3_1:85934889-85934908 None:intergenic 40.0%
!! AAATTGTTGGCTTTAGGCTC+CGG - chr3_1:85934948-85934967 Msa0383160:five_prime_UTR 40.0%
TAACCTCGCCGCTAAATTGT+CGG + chr3_1:85932000-85932019 None:intergenic 45.0%
! CGATTCCGATTTGCTCGATT+TGG + chr3_1:85932229-85932248 None:intergenic 45.0%
TGAGGGTCAGAAAATTGAGG+CGG + chr3_1:85932345-85932364 None:intergenic 45.0%
GGTTGAGGGTCAGAAAATTG+AGG + chr3_1:85932348-85932367 None:intergenic 45.0%
GAGCAAACCTCAAAATGCTC+CGG + chr3_1:85932384-85932403 None:intergenic 45.0%
!!! GTGCATTTTGTCGCTTTCGA+TGG + chr3_1:85932807-85932826 None:intergenic 45.0%
TTACCATACAAGGGTGGTGT+GGG + chr3_1:85932941-85932960 None:intergenic 45.0%
! TCGTCAAATTTCGTCGGTTG+CGG - chr3_1:85932975-85932994 Msa0383160:CDS 45.0%
CAAGTATGCACATGCACAAC+AGG + chr3_1:85933140-85933159 None:intergenic 45.0%
CTGTTGTGCATGTGCATACT+TGG - chr3_1:85933138-85933157 Msa0383160:intron 45.0%
GTGCAGTATGCATGTCAGAA+AGG - chr3_1:85933514-85933533 Msa0383160:intron 45.0%
GGACAGAGAATACCATCTGT+AGG - chr3_1:85933571-85933590 Msa0383160:intron 45.0%
CAGAGACTGTTTCCTACAGA+TGG + chr3_1:85933586-85933605 None:intergenic 45.0%
CATCTGTAGGAAACAGTCTC+TGG - chr3_1:85933584-85933603 Msa0383160:intron 45.0%
! TTACCTCCGTTTGTTTGGTG+TGG - chr3_1:85933810-85933829 Msa0383160:intron 45.0%
CGATAGCCACACCAAACAAA+CGG + chr3_1:85933819-85933838 None:intergenic 45.0%
!! CGTTTGTTTGGTGTGGCTAT+CGG - chr3_1:85933817-85933836 Msa0383160:intron 45.0%
TTAACAGGATACTGAGAGCG+AGG - chr3_1:85933891-85933910 Msa0383160:intron 45.0%
AGAGCGACAACTCTCCAAAA+AGG - chr3_1:85934041-85934060 Msa0383160:intron 45.0%
! TAGAGCAAAGGTACGAGTGA+CGG + chr3_1:85934246-85934265 None:intergenic 45.0%
GGGCAAAAAGAGGGGAAAAT+GGG + chr3_1:85934321-85934340 None:intergenic 45.0%
AGGGCAAAAAGAGGGGAAAA+TGG + chr3_1:85934322-85934341 None:intergenic 45.0%
AACCAGCTAGGGCAAAAAGA+GGG + chr3_1:85934330-85934349 None:intergenic 45.0%
AAACCAGCTAGGGCAAAAAG+AGG + chr3_1:85934331-85934350 None:intergenic 45.0%
!! TGTATTAGCAGGAGTACCTC+AGG - chr3_1:85934484-85934503 Msa0383160:intron 45.0%
! GGAGTACCTCAGGATAAGAA+AGG - chr3_1:85934494-85934513 Msa0383160:intron 45.0%
TTCTTGGCATTCTTGGACTC+GGG + chr3_1:85934560-85934579 None:intergenic 45.0%
CTTCTTGGCATTCTTGGACT+CGG + chr3_1:85934561-85934580 None:intergenic 45.0%
GAAGGATAAAGCTTCCAAGG+AGG - chr3_1:85934589-85934608 Msa0383160:intron 45.0%
!! TTGTTTCTGAGCTGTTGGGA+TGG + chr3_1:85934631-85934650 None:intergenic 45.0%
AATGGGACTGATATGGCTAC+AGG - chr3_1:85934653-85934672 Msa0383160:CDS 45.0%
! GCTTGCTGCAGCAAAGAAAA+AGG - chr3_1:85934823-85934842 Msa0383160:CDS 45.0%
!!! TCTTTTACCGCACTGGTTGT+TGG + chr3_1:85934865-85934884 None:intergenic 45.0%
GCTCTCCTTTACATCAGTAC+AGG - chr3_1:85934890-85934909 Msa0383160:CDS 45.0%
TGGAATACGACTAGAGTCAC+CGG + chr3_1:85934970-85934989 None:intergenic 45.0%
CATACACGACATAAGAACCC+CGG + chr3_1:85935112-85935131 None:intergenic 45.0%
!! GGAAAGTGTTGTCGCTTCTA+TGG - chr3_1:85935170-85935189 Msa0383160:five_prime_UTR 45.0%
!!! TTGATTAAATTAATTTTTTT+TGG - chr3_1:85931874-85931893 Msa0383160:three_prime_UTR 5.0%
CGCTGCAAAAACGTTGGTGT+TGG + chr3_1:85932148-85932167 None:intergenic 50.0%
! ATCGAGCGCTGCAAAAACGT+TGG + chr3_1:85932154-85932173 None:intergenic 50.0%
CAAATCGGAATCGGAATCGC+AGG - chr3_1:85932236-85932255 Msa0383160:CDS 50.0%
! AATCGGAATCGCAGGCGTTT+TGG - chr3_1:85932244-85932263 Msa0383160:CDS 50.0%
! ATCGGAATCGCAGGCGTTTT+GGG - chr3_1:85932245-85932264 Msa0383160:CDS 50.0%
GCCGTTGAATGCAACCTCAT+GGG - chr3_1:85932275-85932294 Msa0383160:CDS 50.0%
GCCCATGAGGTTGCATTCAA+CGG + chr3_1:85932279-85932298 None:intergenic 50.0%
GTCATCAACATCAGCCCATG+AGG + chr3_1:85932292-85932311 None:intergenic 50.0%
GTCAGAAAATTGAGGCGGTG+CGG + chr3_1:85932340-85932359 None:intergenic 50.0%
! GTTTGTCCTCACTGGGTTGA+GGG + chr3_1:85932362-85932381 None:intergenic 50.0%
TCAACCCAGTGAGGACAAAC+CGG - chr3_1:85932362-85932381 Msa0383160:intron 50.0%
!! GGACAAACCGGAGCATTTTG+AGG - chr3_1:85932374-85932393 Msa0383160:intron 50.0%
CAAGGGTGGTGTGGGAAAAT+AGG + chr3_1:85932933-85932952 None:intergenic 50.0%
TTTCCCACACCACCCTTGTA+TGG - chr3_1:85932935-85932954 Msa0383160:CDS 50.0%
CTTACCATACAAGGGTGGTG+TGG + chr3_1:85932942-85932961 None:intergenic 50.0%
!! TGTTTGCTATGCTGTCTGCC+TGG + chr3_1:85933381-85933400 None:intergenic 50.0%
TAGCCACACCAAACAAACGG+AGG + chr3_1:85933816-85933835 None:intergenic 50.0%
! TGGTGTGGCTATCGGTTGAA+TGG - chr3_1:85933825-85933844 Msa0383160:intron 50.0%
! AGATGACGGTGATGAAGAGG+AGG - chr3_1:85933924-85933943 Msa0383160:intron 50.0%
! TTCACAGCATGCTCTGGTTC+TGG + chr3_1:85933967-85933986 None:intergenic 50.0%
TCAGTCTTCACAGCATGCTC+TGG + chr3_1:85933973-85933992 None:intergenic 50.0%
TGCTGTGAAGACTGAACCTG+AGG - chr3_1:85933978-85933997 Msa0383160:intron 50.0%
TCAGCGTGCTTCTTAACCTC+AGG + chr3_1:85933997-85934016 None:intergenic 50.0%
! TGAAGTTCCTGTGGTGCCAA+AGG - chr3_1:85934014-85934033 Msa0383160:intron 50.0%
CAAGCTCAAGATGAGTCGCA+AGG - chr3_1:85934144-85934163 Msa0383160:intron 50.0%
ACCAGCTAGGGCAAAAAGAG+GGG + chr3_1:85934329-85934348 None:intergenic 50.0%
! GGTGTTGAAGGGGATGTTGA+TGG - chr3_1:85934515-85934534 Msa0383160:intron 50.0%
!! GGCTACAGGCACCGAAAATT+CGG - chr3_1:85934667-85934686 Msa0383160:CDS 50.0%
!! TACAGGCACCGAAAATTCGG+AGG - chr3_1:85934670-85934689 Msa0383160:CDS 50.0%
AAGCTGCTGCTAGGAATGCA+AGG - chr3_1:85934801-85934820 Msa0383160:CDS 50.0%
! GCAAGGTTCTTTTACCGCAC+TGG + chr3_1:85934872-85934891 None:intergenic 50.0%
GAACCGACAATTTAGCGGCG+AGG - chr3_1:85931994-85932013 Msa0383160:three_prime_UTR 55.0%
CCAACGAACCCTCATCCCTT+CGG + chr3_1:85932119-85932138 None:intergenic 55.0%
CGCCGTTGAATGCAACCTCA+TGG - chr3_1:85932274-85932293 Msa0383160:CDS 55.0%
AGAAAATTGAGGCGGTGCGG+TGG + chr3_1:85932337-85932356 None:intergenic 55.0%
TTCTGACCCTCAACCCAGTG+AGG - chr3_1:85932353-85932372 Msa0383160:intron 55.0%
GGTTTGTCCTCACTGGGTTG+AGG + chr3_1:85932363-85932382 None:intergenic 55.0%
! TGCTCCGGTTTGTCCTCACT+GGG + chr3_1:85932369-85932388 None:intergenic 55.0%
! ATGCTCCGGTTTGTCCTCAC+TGG + chr3_1:85932370-85932389 None:intergenic 55.0%
GAAGCACGCTGAAGTTCCTG+TGG - chr3_1:85934005-85934024 Msa0383160:intron 55.0%
! AGTTCCTGTGGTGCCAAAGG+AGG - chr3_1:85934017-85934036 Msa0383160:intron 55.0%
AGTTGTCGCTCTGCCTCCTT+TGG + chr3_1:85934033-85934052 None:intergenic 55.0%
! TCCCCTCTTTTTGCCCTAGC+TGG - chr3_1:85934325-85934344 Msa0383160:intron 55.0%
!! AGGCACCGAAAATTCGGAGG+AGG - chr3_1:85934673-85934692 Msa0383160:CDS 55.0%
CTGCTCAAGAAGCTGCTGCT+AGG - chr3_1:85934792-85934811 Msa0383160:CDS 55.0%
!! TGGTTGGTGGTGGAAGCCGA+AGG - chr3_1:85932100-85932119 Msa0383160:three_prime_UTR 60.0%
!! GGTTGGTGGTGGAAGCCGAA+GGG - chr3_1:85932101-85932120 Msa0383160:three_prime_UTR 60.0%
TGGTGGAAGCCGAAGGGATG+AGG - chr3_1:85932107-85932126 Msa0383160:three_prime_UTR 60.0%
GGTGGAAGCCGAAGGGATGA+GGG - chr3_1:85932108-85932127 Msa0383160:three_prime_UTR 60.0%
CCGAAGGGATGAGGGTTCGT+TGG - chr3_1:85932116-85932135 Msa0383160:three_prime_UTR 60.0%
!! ATCGCAGGCGTTTTGGGCTC+CGG - chr3_1:85932251-85932270 Msa0383160:CDS 60.0%
GAGGTTGCATTCAACGGCGC+CGG + chr3_1:85932273-85932292 None:intergenic 60.0%
GCGGTTCGTACCGCATAGCA+TGG - chr3_1:85932994-85933013 Msa0383160:CDS 60.0%
TCTGCCTCCTTTGGCACCAC+AGG + chr3_1:85934024-85934043 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 85931795 85935224 85931795 ID=Msa0383160;Name=Msa0383160
chr3_1 mRNA 85931795 85935224 85931795 ID=Msa0383160-mRNA-1;Parent=Msa0383160;Name=Msa0383160-mRNA-1;_AED=0.04;_eAED=0.04;_QI=276|0.5|0.6|1|0|0|5|344|281
chr3_1 exon 85931795 85932327 85931795 ID=Msa0383160-mRNA-1:exon:22671;Parent=Msa0383160-mRNA-1
chr3_1 exon 85932409 85932524 85932409 ID=Msa0383160-mRNA-1:exon:22670;Parent=Msa0383160-mRNA-1
chr3_1 exon 85932878 85933121 85932878 ID=Msa0383160-mRNA-1:exon:22669;Parent=Msa0383160-mRNA-1
chr3_1 exon 85934624 85935010 85934624 ID=Msa0383160-mRNA-1:exon:22668;Parent=Msa0383160-mRNA-1
chr3_1 exon 85935039 85935224 85935039 ID=Msa0383160-mRNA-1:exon:22667;Parent=Msa0383160-mRNA-1
chr3_1 five_prime_UTR 85935039 85935224 85935039 ID=Msa0383160-mRNA-1:five_prime_utr;Parent=Msa0383160-mRNA-1
chr3_1 five_prime_UTR 85934921 85935010 85934921 ID=Msa0383160-mRNA-1:five_prime_utr;Parent=Msa0383160-mRNA-1
chr3_1 CDS 85934624 85934920 85934624 ID=Msa0383160-mRNA-1:cds;Parent=Msa0383160-mRNA-1
chr3_1 CDS 85932878 85933121 85932878 ID=Msa0383160-mRNA-1:cds;Parent=Msa0383160-mRNA-1
chr3_1 CDS 85932409 85932524 85932409 ID=Msa0383160-mRNA-1:cds;Parent=Msa0383160-mRNA-1
chr3_1 CDS 85932139 85932327 85932139 ID=Msa0383160-mRNA-1:cds;Parent=Msa0383160-mRNA-1
chr3_1 three_prime_UTR 85931795 85932138 85931795 ID=Msa0383160-mRNA-1:three_prime_utr;Parent=Msa0383160-mRNA-1
Gene Sequence

>Msa0383160

ATGGTTGGTGGTGGAAGCCGAAGGGATGAGGGTTCGTTGGTGATTTCCAACACCAACGTTTTTGCAGCGCTCGATACTCTGAAGAAGAAGAAGAAATCAGACAAGGACAAGAAGAATAAAGGTTCTTCCAAATCGAGCAAATCGGAATCGGAATCGCAGGCGTTTTGGGCTCCGGCGCCGTTGAATGCAACCTCATGGGCTGATGTTGATGACGATGATGATGATTATTATCTGACCACCGCACCGCCTCAATTTTCTGACCCTCAACCCAGTGAGGACAAACCGGAGCATTTTGAGGATACTGAGAGCGAGGAAGATATTTTAGATGACGGTGATGAAGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGCATGCTGTGAAGACTGAACCTGAGGTTAAGAAGCACGCTGAAGTTCCTGTGGTGCCAAAGGAGGCAGAGCGACAACTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTCACTCAGAAAGAAAGTAATGATGGAGTACCTCAGGATAAGAAAGGTGTTGAAGGGGATGTTGATGGAGAAAAGAAAGAAATTGCTCCCGAGTCCAAGAATGCCAAGAAGAAGAAAAAGAAGGATAAAGCTTCCAAGGAGGAGGATCTTTCTGTTGTTGACATGAAAGAACGTCTTAAGAAAGTTGCTTCTATGAAGAAGAAGAAATCAAGTAAAGAAATGGATGCTGCAGCTAGAGCTGCTGCTCAAGAAGCTGCTGCTAGGAATGCAAGGCTTGCTGCAGCAAAGAAAAAGGAGAAGAACCATTATAACCAACAACCAGTGCGGTAA
Protein sequence

>Msa0383160

MVGGGSRRDEGSLVISNTNVFAALDTLKKKKKSDKDKKNKGSSKSSKSESESQAFWAPAPLNATSWADVDDDDDDYYLTTAPPQFSDPQPSEDKPEHFEDTESEEDILDDGDEEEEEQDHEPEPEHAVKTEPEVKKHAEVPVVPKEAERQLSKKERKKKELEELEALLADFGVTQKESNDGVPQDKKGVEGDVDGEKKEIAPESKNAKKKKKKDKASKEEDLSVVDMKERLKKVASMKKKKSSKEMDAAARAAAQEAAARNARLAAAKKKEKNHYNQQPVR*