AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0388880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAACTTAGAACAGAATT+TGG 0.242473 3_1:-93351022 None:intergenic
AAATGATATTAGCAATCATT+TGG 0.401830 3_1:+93351149 None:intergenic
GAGTGGCAACATAAGATTCA+TGG 0.443820 3_1:-93351275 Msa0388880:CDS
AAGCTTCTTTGGAACTCTGA+AGG 0.525980 3_1:-93353464 Msa0388880:intron
CATTTGGAGTGCAATCAGAA+AGG 0.530740 3_1:+93351165 None:intergenic
TCAATCACAACCTTGGTGGC+AGG 0.533781 3_1:+93351115 None:intergenic
CATGAGCTGAGCAAAACTAC+TGG 0.538533 3_1:-93352857 Msa0388880:CDS
AGCAAAACTACTGGAAATGC+AGG 0.541389 3_1:-93352848 Msa0388880:CDS
AGTGGCAACATAAGATTCAT+GGG 0.563520 3_1:-93351274 Msa0388880:CDS
ATCAACAACGACCTTGGTGG+CGG 0.572454 3_1:-93351238 Msa0388880:CDS
CATATCAATCACAACCTTGG+TGG 0.578162 3_1:+93351111 None:intergenic
CATATCAACAACGACCTTGG+TGG 0.587061 3_1:-93351241 Msa0388880:CDS
AGACATATCAACAACGACCT+TGG 0.591658 3_1:-93351244 Msa0388880:CDS
TCTTGTAGCAAGATCATCAA+AGG 0.594386 3_1:-93351472 Msa0388880:intron
TGGCGGTTACATCATCATGA+AGG 0.595175 3_1:-93351221 Msa0388880:CDS
AGACATATCAATCACAACCT+TGG 0.611500 3_1:+93351108 None:intergenic
TGATAATGTACCTGCCACCA+AGG 0.660264 3_1:-93351125 Msa0388880:CDS
TGATGATGTAACCGCCACCA+AGG 0.665434 3_1:+93351227 None:intergenic
ACGGACAACTACATCAAGAG+TGG 0.672547 3_1:-93351292 Msa0388880:CDS
GCAGGTGCAAGAGTAGCATG+CGG 0.736736 3_1:-93352830 Msa0388880:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTATTTATAATTTAATAC+CGG - chr3_1:93351271-93351290 Msa0388880:CDS 10.0%
!! CGGTATTAAATTATAAATAA+AGG + chr3_1:93351273-93351292 None:intergenic 15.0%
!! ATAGAAAAAATAAGAATGAA+AGG - chr3_1:93351387-93351406 Msa0388880:intron 15.0%
!! TAAAAAAAAAGCTCAAAAAT+TGG + chr3_1:93351610-93351629 None:intergenic 15.0%
!!! TTTGAGCTTTTTTTTTATAA+TGG - chr3_1:93351613-93351632 Msa0388880:intron 15.0%
!! TTCATCAATAATATGAATAA+TGG + chr3_1:93351707-93351726 None:intergenic 15.0%
!! TTCTTATCAAAATATTAGAT+TGG - chr3_1:93351818-93351837 Msa0388880:intron 15.0%
!!! TTTATAATTTAATACCGGTT+AGG - chr3_1:93351276-93351295 Msa0388880:CDS 20.0%
!! ATTTAAGAAGTTTGTATGAA+AGG - chr3_1:93351423-93351442 Msa0388880:intron 20.0%
!! TTTATTGAATGATTGAGAAT+AGG + chr3_1:93351492-93351511 None:intergenic 20.0%
!!! CTTTTTTTTTATAATGGAAC+AGG - chr3_1:93351619-93351638 Msa0388880:intron 20.0%
!! AGTAGATTATGATTTATTAC+TGG + chr3_1:93351739-93351758 None:intergenic 20.0%
!! TGAATTATTGAAATGTTGTA+TGG + chr3_1:93351904-93351923 None:intergenic 20.0%
!!! GTTGGATTAATATTTTATCT+CGG - chr3_1:93351984-93352003 Msa0388880:intron 20.0%
!!! ACATGATTGTAATTTTCATA+GGG + chr3_1:93352353-93352372 None:intergenic 20.0%
!!! AACATGATTGTAATTTTCAT+AGG + chr3_1:93352354-93352373 None:intergenic 20.0%
!! TTTGATCTTATACAAATTTC+AGG - chr3_1:93352495-93352514 Msa0388880:intron 20.0%
!! ACGAAGAAAATAAAAGTTAA+TGG - chr3_1:93352874-93352893 Msa0388880:intron 20.0%
!! AAAGTTAATGGAAATCTAAA+AGG - chr3_1:93352886-93352905 Msa0388880:intron 20.0%
!! AAGTTAATGGAAATCTAAAA+GGG - chr3_1:93352887-93352906 Msa0388880:intron 20.0%
!! AAATGATATTAGCAATCATT+TGG + chr3_1:93353357-93353376 None:intergenic 20.0%
!!! TTTTTTTATAATGGAACAGG+TGG - chr3_1:93351622-93351641 Msa0388880:intron 25.0%
! ATTCAGTAAGTAAATGAAGT+AGG - chr3_1:93351919-93351938 Msa0388880:intron 25.0%
! TAATCTTAATATGAGAGGAA+TGG - chr3_1:93352073-93352092 Msa0388880:intron 25.0%
!! CTTTGAAGATCTATTATTTG+TGG + chr3_1:93352304-93352323 None:intergenic 25.0%
! TTTGGGTCGTAATTAAATAT+TGG - chr3_1:93352736-93352755 Msa0388880:intron 25.0%
!! TTCTTCTTTTGATATGAAGA+CGG - chr3_1:93353192-93353211 Msa0388880:intron 25.0%
!!! GAAGGATTTTAACAATCATT+TGG - chr3_1:93353300-93353319 Msa0388880:intron 25.0%
!! TTCTTCTTTTGATATGAAGA+CGG + chr3_1:93353192-93353211 None:intergenic 25.0%
AACACAACTAACATGTCAAT+GGG + chr3_1:93351097-93351116 None:intergenic 30.0%
TTGAATATAATCACACGTGT+TGG + chr3_1:93351161-93351180 None:intergenic 30.0%
AGAGAAGCTATATAGTTAGA+AGG - chr3_1:93351358-93351377 Msa0388880:intron 30.0%
GAGAAGCTATATAGTTAGAA+GGG - chr3_1:93351359-93351378 Msa0388880:intron 30.0%
AAAGGAAAAATCCTTTGTGA+AGG - chr3_1:93351441-93351460 Msa0388880:intron 30.0%
AATTTACTACTTCCTCTCTT+TGG - chr3_1:93351541-93351560 Msa0388880:intron 30.0%
CATCATGACATAAACTCAAA+CGG + chr3_1:93351860-93351879 None:intergenic 30.0%
CAGTACCATGATTAATCTAA+AGG + chr3_1:93352010-93352029 None:intergenic 30.0%
TTAGATTAATCATGGTACTG+AGG - chr3_1:93352010-93352029 Msa0388880:intron 30.0%
CCTCTCATATTAAGATTATC+CGG + chr3_1:93352071-93352090 None:intergenic 30.0%
ACATTTCTGTCTTATGCTAA+CGG + chr3_1:93352173-93352192 None:intergenic 30.0%
CAAATGTTGAAACTACTCAA+GGG + chr3_1:93352479-93352498 None:intergenic 30.0%
TCAAATGTTGAAACTACTCA+AGG + chr3_1:93352480-93352499 None:intergenic 30.0%
TACAATACACCAAAAATGGT+GGG + chr3_1:93352613-93352632 None:intergenic 30.0%
TGTGTACAATACACCAAAAA+TGG + chr3_1:93352617-93352636 None:intergenic 30.0%
GATGATCGAAACATGTATTT+CGG - chr3_1:93352837-93352856 Msa0388880:CDS 30.0%
TGAAAGACTTTCTTCTTCAA+AGG + chr3_1:93353123-93353142 None:intergenic 30.0%
! GCTTTTCTTCTAAGCTTCTT+TGG - chr3_1:93351028-93351047 Msa0388880:CDS 35.0%
CAACACAACTAACATGTCAA+TGG + chr3_1:93351098-93351117 None:intergenic 35.0%
ACATCTTCAATGTGAAGTGT+CGG + chr3_1:93351123-93351142 None:intergenic 35.0%
ATTGAAGATGTATCCAATGC+CGG - chr3_1:93351132-93351151 Msa0388880:CDS 35.0%
! TTTAATACCGGTTAGGATAG+AGG - chr3_1:93351283-93351302 Msa0388880:CDS 35.0%
! TTAATACCGGTTAGGATAGA+GGG - chr3_1:93351284-93351303 Msa0388880:CDS 35.0%
CAATAATTCCACCTTCACAA+AGG + chr3_1:93351455-93351474 None:intergenic 35.0%
TGTGAAGGTGGAATTATTGA+AGG - chr3_1:93351456-93351475 Msa0388880:intron 35.0%
TGAATGATTGAGAATAGGAG+AGG + chr3_1:93351487-93351506 None:intergenic 35.0%
GTTGTTATGAATCCAAAGAG+AGG + chr3_1:93351556-93351575 None:intergenic 35.0%
ATGTTGTATGGTATGAGTCA+AGG + chr3_1:93351892-93351911 None:intergenic 35.0%
! GTAAATGAAGTAGGTTGACT+TGG - chr3_1:93351928-93351947 Msa0388880:intron 35.0%
CCGGATAATCTTAATATGAG+AGG - chr3_1:93352068-93352087 Msa0388880:intron 35.0%
CAGCTATAGAGAAATCAACT+TGG + chr3_1:93352134-93352153 None:intergenic 35.0%
AACTATCCTAAACAGATGAC+TGG + chr3_1:93352278-93352297 None:intergenic 35.0%
! AGTCATCTGTTTAGGATAGT+TGG - chr3_1:93352277-93352296 Msa0388880:intron 35.0%
! TCAAGTTGTTCTATGCGTAA+TGG - chr3_1:93352424-93352443 Msa0388880:intron 35.0%
TGTTCTATGCGTAATGGTTT+CGG - chr3_1:93352430-93352449 Msa0388880:intron 35.0%
! AAGAATAGAAAAGTGCAACC+CGG - chr3_1:93352547-93352566 Msa0388880:intron 35.0%
GTACAATACACCAAAAATGG+TGG + chr3_1:93352614-93352633 None:intergenic 35.0%
!!! TACCCTGTTTTTGACATAAG+AGG - chr3_1:93352641-93352660 Msa0388880:intron 35.0%
AGCCTCTTATGTCAAAAACA+GGG + chr3_1:93352646-93352665 None:intergenic 35.0%
AAATTTAACCTTGGCACAAC+TGG + chr3_1:93352719-93352738 None:intergenic 35.0%
AGTTGTGCCAAGGTTAAATT+TGG - chr3_1:93352718-93352737 Msa0388880:intron 35.0%
GTTGTGCCAAGGTTAAATTT+GGG - chr3_1:93352719-93352738 Msa0388880:intron 35.0%
TTACGACCCAAATTTAACCT+TGG + chr3_1:93352728-93352747 None:intergenic 35.0%
GTTTCTCAAATCTCACTTGT+CGG + chr3_1:93352762-93352781 None:intergenic 35.0%
AGAATACTTGAGACAAGCAT+GGG + chr3_1:93352925-93352944 None:intergenic 35.0%
AAGAATACTTGAGACAAGCA+TGG + chr3_1:93352926-93352945 None:intergenic 35.0%
TCTTGTAGCAAGATCATCAA+AGG - chr3_1:93353031-93353050 Msa0388880:intron 35.0%
! TCAAAGGAGAGTTTTCAACA+TGG + chr3_1:93353107-93353126 None:intergenic 35.0%
AGTGGCAACATAAGATTCAT+GGG - chr3_1:93353229-93353248 Msa0388880:intron 35.0%
AGACATATCAATCACAACCT+TGG + chr3_1:93353398-93353417 None:intergenic 35.0%
!! AAGCTTCTTTGGAACTCTGA+AGG - chr3_1:93351039-93351058 Msa0388880:CDS 40.0%
CGACATTGACACATTAACAC+TGG + chr3_1:93351207-93351226 None:intergenic 40.0%
GGAAAAATCCTTTGTGAAGG+TGG - chr3_1:93351444-93351463 Msa0388880:intron 40.0%
AGCAAAACTACTGGAAATGC+AGG - chr3_1:93351655-93351674 Msa0388880:intron 40.0%
TTGTATGGTATGAGTCAAGG+AGG + chr3_1:93351889-93351908 None:intergenic 40.0%
CAGCCTCTCAAGTCAAATTT+CGG - chr3_1:93351959-93351978 Msa0388880:intron 40.0%
TCAAGTCAAATTTCGGCTGT+TGG - chr3_1:93351966-93351985 Msa0388880:intron 40.0%
CTCGGCCTTTAGATTAATCA+TGG - chr3_1:93352002-93352021 Msa0388880:intron 40.0%
! TTAATCATGGTACTGAGGTG+TGG - chr3_1:93352015-93352034 Msa0388880:intron 40.0%
ACAATTGCTAATTGCTGACC+AGG - chr3_1:93352240-93352259 Msa0388880:intron 40.0%
AATTGCTGACCAGGTATCAT+TGG - chr3_1:93352249-93352268 Msa0388880:intron 40.0%
TGTTGAAACTACTCAAGGGA+AGG + chr3_1:93352475-93352494 None:intergenic 40.0%
CAGCCTCTTATGTCAAAAAC+AGG + chr3_1:93352647-93352666 None:intergenic 40.0%
GTTGTTGTCATGTGACTGAA+AGG + chr3_1:93352692-93352711 None:intergenic 40.0%
CTAGCAAATGGAAGATTTGG+AGG + chr3_1:93352964-93352983 None:intergenic 40.0%
CGACTAGCAAATGGAAGATT+TGG + chr3_1:93352967-93352986 None:intergenic 40.0%
TTCTGATCTCGACTAGCAAA+TGG + chr3_1:93352976-93352995 None:intergenic 40.0%
TCAACATGGTAGGACATAGA+AGG + chr3_1:93353093-93353112 None:intergenic 40.0%
! AGGAGAGTTTTCAACATGGT+AGG + chr3_1:93353103-93353122 None:intergenic 40.0%
GAGTGGCAACATAAGATTCA+TGG - chr3_1:93353228-93353247 Msa0388880:intron 40.0%
AGACATATCAACAACGACCT+TGG - chr3_1:93353259-93353278 Msa0388880:intron 40.0%
! CATTTGGAGTGCAATCAGAA+AGG + chr3_1:93353341-93353360 None:intergenic 40.0%
CATATCAATCACAACCTTGG+TGG + chr3_1:93353395-93353414 None:intergenic 40.0%
! TAATCACACGTGTTGGTGTC+CGG + chr3_1:93351154-93351173 None:intergenic 45.0%
GATTCTCCCTCTATCCTAAC+CGG + chr3_1:93351293-93351312 None:intergenic 45.0%
CATGAGCTGAGCAAAACTAC+TGG - chr3_1:93351646-93351665 Msa0388880:intron 45.0%
! TTGACTTGAGAGGCTGCATA+GGG + chr3_1:93351955-93351974 None:intergenic 45.0%
!! TTTGACTTGAGAGGCTGCAT+AGG + chr3_1:93351956-93351975 None:intergenic 45.0%
!! CAGCCGAAATTTGACTTGAG+AGG + chr3_1:93351965-93351984 None:intergenic 45.0%
CTTATGCTAACGGTCTACAG+CGG + chr3_1:93352163-93352182 None:intergenic 45.0%
TGGACTCCAGTCATCTGTTT+AGG - chr3_1:93352269-93352288 Msa0388880:intron 45.0%
AAGGCAAACTAAGGTTGCCT+TGG + chr3_1:93352456-93352475 None:intergenic 45.0%
ACTCAAGGGAAGGCAAACTA+AGG + chr3_1:93352465-93352484 None:intergenic 45.0%
! TGACATAAGAGGCTGTTTCC+AGG - chr3_1:93352652-93352671 Msa0388880:intron 45.0%
TCATGTGACTGAAAGGTCAC+GGG + chr3_1:93352685-93352704 None:intergenic 45.0%
GTCATGTGACTGAAAGGTCA+CGG + chr3_1:93352686-93352705 None:intergenic 45.0%
CAACTTTACCAGTTGTGCCA+AGG - chr3_1:93352708-93352727 Msa0388880:intron 45.0%
ACGGACAACTACATCAAGAG+TGG - chr3_1:93353211-93353230 Msa0388880:intron 45.0%
CATATCAACAACGACCTTGG+TGG - chr3_1:93353262-93353281 Msa0388880:intron 45.0%
TGGCGGTTACATCATCATGA+AGG - chr3_1:93353282-93353301 Msa0388880:intron 45.0%
! TGATAATGTACCTGCCACCA+AGG - chr3_1:93353378-93353397 Msa0388880:intron 45.0%
!! CTTTGGAACTCTGAAGGTCC+AGG - chr3_1:93351045-93351064 Msa0388880:CDS 50.0%
AACAGAGCAGTAGTCCATCC+TGG + chr3_1:93351066-93351085 None:intergenic 50.0%
TCCGATGTCCATGTCTGCAT+CGG - chr3_1:93351229-93351248 Msa0388880:CDS 50.0%
ACCGATGCAGACATGGACAT+CGG + chr3_1:93351233-93351252 None:intergenic 50.0%
AGATTATCCGGCTTCCACAC+TGG + chr3_1:93352059-93352078 None:intergenic 50.0%
GACTGGAGTCCAATGATACC+TGG + chr3_1:93352261-93352280 None:intergenic 50.0%
ATGCGTAATGGTTTCGGCCA+AGG - chr3_1:93352436-93352455 Msa0388880:intron 50.0%
ATCAACAACGACCTTGGTGG+CGG - chr3_1:93353265-93353284 Msa0388880:intron 50.0%
! TGATGATGTAACCGCCACCA+AGG + chr3_1:93353279-93353298 None:intergenic 50.0%
! TCAATCACAACCTTGGTGGC+AGG + chr3_1:93353391-93353410 None:intergenic 50.0%
! GGAACTCTGAAGGTCCAGGA+TGG - chr3_1:93351049-93351068 Msa0388880:CDS 55.0%
!! ACGAAGCACCGATGCAGACA+TGG + chr3_1:93351240-93351259 None:intergenic 55.0%
GCAGGTGCAAGAGTAGCATG+CGG - chr3_1:93351673-93351692 Msa0388880:intron 55.0%
TTCCACACTGGATCCTCTGC+AGG + chr3_1:93352047-93352066 None:intergenic 55.0%
AGAGGATCCAGTGTGGAAGC+CGG - chr3_1:93352049-93352068 Msa0388880:intron 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr3_1:93352569-93352588 None:intergenic 55.0%
ACTAAAGCTCCCGCATACGC+AGG - chr3_1:93352573-93352592 Msa0388880:intron 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr3_1:93352574-93352593 Msa0388880:intron 55.0%
! AAAATGGTGGGACCCCTTCC+CGG + chr3_1:93352601-93352620 None:intergenic 55.0%
! GGAAGGGGTCCCACCATTTT+TGG - chr3_1:93352601-93352620 Msa0388880:intron 55.0%
AAGGTCACGGGTTCAAGTCC+TGG + chr3_1:93352673-93352692 None:intergenic 55.0%
! CACGTGTTGGTGTCCGGCAT+TGG + chr3_1:93351148-93351167 None:intergenic 60.0%
AGCCTGCAGAGGATCCAGTG+TGG - chr3_1:93352042-93352061 Msa0388880:intron 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr3_1:93352568-93352587 None:intergenic 60.0%
GGTGTGGCTAGAGCCTGCAG+AGG - chr3_1:93352031-93352050 Msa0388880:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG + chr3_1:93352585-93352604 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG + chr3_1:93352586-93352605 None:intergenic 65.0%
GCATACGCAGGGTCCGGGAA+GGG - chr3_1:93352585-93352604 Msa0388880:intron 65.0%
CATACGCAGGGTCCGGGAAG+GGG - chr3_1:93352586-93352605 Msa0388880:intron 65.0%
GCTCCCGCATACGCAGGGTC+CGG - chr3_1:93352579-93352598 Msa0388880:intron 70.0%
CTCCCGCATACGCAGGGTCC+GGG - chr3_1:93352580-93352599 Msa0388880:intron 70.0%
CGCATACGCAGGGTCCGGGA+AGG - chr3_1:93352584-93352603 Msa0388880:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 93351024 93353501 93351024 ID=Msa0388880;Name=Msa0388880
chr3_1 mRNA 93351024 93353501 93351024 ID=Msa0388880-mRNA-1;Parent=Msa0388880;Name=Msa0388880-mRNA-1;_AED=0.91;_eAED=0.91;_QI=0|0|0.25|0.25|0.66|0.5|4|0|132
chr3_1 exon 93353465 93353501 93353465 ID=Msa0388880-mRNA-1:exon:26430;Parent=Msa0388880-mRNA-1
chr3_1 exon 93352831 93352884 93352831 ID=Msa0388880-mRNA-1:exon:26429;Parent=Msa0388880-mRNA-1
chr3_1 exon 93351473 93351486 93351473 ID=Msa0388880-mRNA-1:exon:26428;Parent=Msa0388880-mRNA-1
chr3_1 exon 93351024 93351314 93351024 ID=Msa0388880-mRNA-1:exon:26427;Parent=Msa0388880-mRNA-1
chr3_1 CDS 93353465 93353501 93353465 ID=Msa0388880-mRNA-1:cds;Parent=Msa0388880-mRNA-1
chr3_1 CDS 93352831 93352884 93352831 ID=Msa0388880-mRNA-1:cds;Parent=Msa0388880-mRNA-1
chr3_1 CDS 93351473 93351486 93351473 ID=Msa0388880-mRNA-1:cds;Parent=Msa0388880-mRNA-1
chr3_1 CDS 93351024 93351314 93351024 ID=Msa0388880-mRNA-1:cds;Parent=Msa0388880-mRNA-1
Gene Sequence

>Msa0388880

ATGTGCTTTTCTTCTAAGCTTCTTTGGAACTCTGAAGGTGGACATGAGCTGAGCAAAACTACTGGAAATGCAGGTGCAAGAGTAGCATGCGCAAGATCATCAAAGACGGACAACTACATCAAGAGTGGCAACATAAGATTCATGGGTTACATCAGACATATCAACAACGACCTTGGTGGCGGTTACATCATCATGAAGGATTTTAACAATCATTTGGAGTACAATCAGCAAGCCTTTCTGATTGCACTCCAAATGATTGCTAATATCATTTATGATAATGTACCTGCCACCAAGGTTGTGATTGATATGTCTGATGAAACTCATAAATCTTATGTTGTCGCTCTTGAAGTAGTGAACCGTCTTCATATCAAAAGAAGAAAACTTAGAACAGAATTT

Protein sequence

>Msa0388880

MCFSSKLLWNSEGGHELSKTTGNAGARVACARSSKTDNYIKSGNIRFMGYIRHINNDLGGGYIIMKDFNNHLEYNQQAFLIALQMIANIIYDNVPATKVVIDMSDETHKSYVVALEVVNRLHIKRRKLRTEF