Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0390150 | KEH41883.1 | 96.145 | 441 | 13 | 2 | 1 | 441 | 1 | 437 | 0.0 | 862 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0390150 | sp|Q9SVC6|PUB22_ARATH | 53.829 | 444 | 189 | 6 | 1 | 438 | 1 | 434 | 1.35e-155 | 450 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0390150 | A0A072VKF3 | 96.145 | 441 | 13 | 2 | 1 | 441 | 1 | 437 | 0.0 | 862 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0390150 | Msa0390160 | 0.853086 | 3.014920e-61 | -8.615850e-47 |
| Msa0390150 | Msa0390170 | 0.862600 | 4.518384e-64 | -8.615850e-47 |
| Msa0390150 | Msa0789770 | 0.994157 | 5.221036e-205 | -8.615850e-47 |
| Msa0390150 | Msa0789780 | 0.836047 | 1.176060e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0390150 | MtrunA17_Chr1g0177271 | 95.918 | 441 | 14 | 2 | 1 | 441 | 1 | 437 | 0.0 | 859 |
| Msa0390150 | MtrunA17_Chr1g0177261 | 95.465 | 441 | 16 | 2 | 1 | 441 | 1 | 437 | 0.0 | 858 |
| Msa0390150 | MtrunA17_Chr1g0177281 | 95.692 | 441 | 15 | 2 | 1 | 441 | 1 | 437 | 0.0 | 857 |
| Msa0390150 | MtrunA17_Chr7g0267501 | 64.545 | 440 | 132 | 7 | 1 | 438 | 1 | 418 | 0.0 | 541 |
| Msa0390150 | MtrunA17_Chr2g0322021 | 54.023 | 435 | 166 | 4 | 4 | 438 | 3 | 403 | 4.01e-152 | 437 |
| Msa0390150 | MtrunA17_Chr1g0177301 | 40.972 | 432 | 215 | 8 | 4 | 430 | 3 | 399 | 9.82e-105 | 317 |
| Msa0390150 | MtrunA17_Chr4g0025501 | 36.735 | 441 | 240 | 8 | 4 | 437 | 3 | 411 | 9.16e-90 | 278 |
| Msa0390150 | MtrunA17_Chr3g0110121 | 33.260 | 454 | 235 | 13 | 3 | 437 | 32 | 436 | 2.94e-58 | 197 |
| Msa0390150 | MtrunA17_Chr5g0434171 | 33.028 | 436 | 243 | 15 | 4 | 429 | 32 | 428 | 1.86e-52 | 182 |
| Msa0390150 | MtrunA17_Chr5g0420471 | 31.395 | 430 | 248 | 11 | 2 | 418 | 20 | 415 | 1.41e-51 | 179 |
| Msa0390150 | MtrunA17_Chr6g0479261 | 30.536 | 429 | 253 | 10 | 2 | 418 | 20 | 415 | 2.42e-51 | 179 |
| Msa0390150 | MtrunA17_Chr4g0044631 | 31.368 | 424 | 244 | 11 | 5 | 423 | 12 | 393 | 1.07e-45 | 163 |
| Msa0390150 | MtrunA17_Chr8g0384041 | 28.959 | 442 | 268 | 12 | 5 | 431 | 8 | 418 | 8.12e-35 | 134 |
| Msa0390150 | MtrunA17_Chr3g0129811 | 28.217 | 443 | 268 | 12 | 5 | 431 | 81 | 489 | 2.80e-30 | 122 |
| Msa0390150 | MtrunA17_Chr4g0031431 | 26.097 | 433 | 254 | 15 | 11 | 430 | 259 | 638 | 2.61e-28 | 117 |
| Msa0390150 | MtrunA17_Chr7g0237931 | 26.961 | 408 | 254 | 13 | 11 | 418 | 241 | 604 | 5.65e-27 | 113 |
| Msa0390150 | MtrunA17_Chr1g0199321 | 29.326 | 341 | 180 | 13 | 11 | 339 | 251 | 542 | 1.67e-25 | 109 |
| Msa0390150 | MtrunA17_Chr4g0044411 | 27.246 | 334 | 177 | 9 | 11 | 328 | 277 | 560 | 1.92e-25 | 109 |
| Msa0390150 | MtrunA17_Chr2g0284461 | 28.571 | 322 | 165 | 11 | 11 | 313 | 296 | 571 | 1.81e-24 | 106 |
| Msa0390150 | MtrunA17_Chr7g0248251 | 30.216 | 278 | 167 | 10 | 11 | 274 | 278 | 542 | 5.56e-24 | 104 |
| Msa0390150 | MtrunA17_Chr5g0400971 | 25.416 | 421 | 266 | 9 | 6 | 426 | 66 | 438 | 2.14e-23 | 102 |
| Msa0390150 | MtrunA17_Chr4g0059201 | 28.266 | 421 | 247 | 18 | 11 | 418 | 323 | 701 | 6.16e-23 | 102 |
| Msa0390150 | MtrunA17_Chr5g0437411 | 26.667 | 360 | 187 | 13 | 11 | 342 | 297 | 607 | 1.51e-22 | 100 |
| Msa0390150 | MtrunA17_Chr3g0122721 | 25.427 | 468 | 284 | 18 | 13 | 441 | 286 | 727 | 1.43e-19 | 91.7 |
| Msa0390150 | MtrunA17_Chr5g0413681 | 27.371 | 369 | 233 | 13 | 11 | 375 | 284 | 621 | 8.62e-19 | 89.0 |
| Msa0390150 | MtrunA17_Chr4g0013091 | 24.944 | 445 | 251 | 19 | 2 | 418 | 306 | 695 | 5.19e-18 | 86.7 |
| Msa0390150 | MtrunA17_Chr8g0377151 | 27.273 | 451 | 252 | 23 | 13 | 418 | 288 | 707 | 5.97e-17 | 83.6 |
| Msa0390150 | MtrunA17_Chr7g0234671 | 42.500 | 80 | 39 | 2 | 1 | 80 | 72 | 144 | 5.88e-16 | 79.7 |
| Msa0390150 | MtrunA17_Chr4g0048811 | 27.439 | 328 | 176 | 11 | 11 | 325 | 9 | 287 | 7.14e-16 | 78.6 |
| Msa0390150 | MtrunA17_Chr7g0275081 | 45.745 | 94 | 40 | 4 | 11 | 104 | 82 | 164 | 7.80e-16 | 79.3 |
| Msa0390150 | MtrunA17_Chr7g0261371 | 45.679 | 81 | 37 | 2 | 5 | 85 | 239 | 312 | 1.06e-15 | 79.7 |
| Msa0390150 | MtrunA17_Chr3g0144071 | 26.977 | 215 | 115 | 8 | 11 | 207 | 34 | 224 | 2.27e-15 | 78.6 |
| Msa0390150 | MtrunA17_Chr1g0183781 | 45.455 | 77 | 35 | 2 | 5 | 81 | 239 | 308 | 3.68e-15 | 77.8 |
| Msa0390150 | MtrunA17_Chr1g0152291 | 50.667 | 75 | 30 | 2 | 6 | 80 | 44 | 111 | 4.29e-15 | 77.0 |
| Msa0390150 | MtrunA17_Chr8g0373031 | 44.086 | 93 | 39 | 4 | 5 | 95 | 271 | 352 | 1.38e-14 | 76.3 |
| Msa0390150 | MtrunA17_Chr2g0280671 | 25.581 | 301 | 181 | 7 | 13 | 313 | 21 | 278 | 1.38e-14 | 75.1 |
| Msa0390150 | MtrunA17_Chr3g0117851 | 24.267 | 375 | 240 | 11 | 11 | 374 | 279 | 620 | 1.70e-14 | 75.9 |
| Msa0390150 | MtrunA17_Chr5g0404311 | 42.857 | 98 | 41 | 6 | 1 | 95 | 265 | 350 | 1.43e-13 | 72.8 |
| Msa0390150 | MtrunA17_Chr8g0390311 | 39.326 | 89 | 40 | 3 | 11 | 99 | 399 | 473 | 4.68e-13 | 71.6 |
| Msa0390150 | MtrunA17_Chr3g0106931 | 41.667 | 84 | 42 | 2 | 2 | 85 | 228 | 304 | 7.62e-13 | 70.9 |
| Msa0390150 | MtrunA17_Chr1g0189811 | 39.560 | 91 | 44 | 4 | 5 | 95 | 61 | 140 | 1.05e-12 | 69.7 |
| Msa0390150 | MtrunA17_Chr1g0196911 | 43.243 | 74 | 36 | 2 | 11 | 84 | 510 | 577 | 1.26e-12 | 70.5 |
| Msa0390150 | MtrunA17_Chr7g0275961 | 41.667 | 84 | 42 | 2 | 2 | 85 | 250 | 326 | 5.07e-12 | 68.2 |
| Msa0390150 | MtrunA17_Chr1g0198984 | 45.070 | 71 | 29 | 3 | 20 | 90 | 1 | 61 | 4.41e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0390150 | AT3G52450.1 | 53.829 | 444 | 189 | 6 | 1 | 438 | 1 | 434 | 1.38e-156 | 450 |
| Msa0390150 | AT2G35930.1 | 50.226 | 442 | 179 | 5 | 1 | 438 | 6 | 410 | 1.91e-153 | 441 |
| Msa0390150 | AT3G11840.1 | 34.968 | 469 | 239 | 9 | 3 | 435 | 6 | 444 | 2.96e-78 | 250 |
| Msa0390150 | AT3G19380.1 | 33.643 | 431 | 240 | 9 | 1 | 423 | 8 | 400 | 3.46e-51 | 178 |
| Msa0390150 | AT5G37490.1 | 30.415 | 434 | 255 | 14 | 4 | 429 | 28 | 422 | 7.14e-46 | 164 |
| Msa0390150 | AT1G49780.1 | 31.603 | 443 | 256 | 11 | 1 | 432 | 8 | 414 | 2.01e-44 | 160 |
| Msa0390150 | AT1G66160.1 | 30.211 | 427 | 245 | 15 | 2 | 421 | 26 | 406 | 4.17e-43 | 157 |
| Msa0390150 | AT1G66160.2 | 27.660 | 423 | 239 | 15 | 4 | 421 | 30 | 390 | 1.73e-30 | 122 |
| Msa0390150 | AT2G28830.1 | 28.842 | 423 | 260 | 18 | 2 | 421 | 178 | 562 | 1.08e-29 | 122 |
| Msa0390150 | AT1G23030.1 | 26.794 | 418 | 248 | 14 | 11 | 422 | 245 | 610 | 5.31e-29 | 120 |
| Msa0390150 | AT5G09800.1 | 28.764 | 445 | 256 | 15 | 5 | 431 | 8 | 409 | 3.00e-28 | 115 |
| Msa0390150 | AT3G18710.1 | 27.314 | 443 | 269 | 12 | 5 | 431 | 10 | 415 | 5.49e-27 | 112 |
| Msa0390150 | AT5G42340.1 | 29.392 | 296 | 166 | 8 | 11 | 306 | 294 | 546 | 3.49e-25 | 108 |
| Msa0390150 | AT1G71020.2 | 26.995 | 426 | 246 | 14 | 11 | 422 | 99 | 473 | 6.26e-25 | 107 |
| Msa0390150 | AT1G71020.1 | 26.995 | 426 | 246 | 14 | 11 | 422 | 247 | 621 | 9.93e-25 | 107 |
| Msa0390150 | AT3G46510.1 | 27.166 | 427 | 257 | 18 | 2 | 421 | 250 | 629 | 1.71e-24 | 107 |
| Msa0390150 | AT5G64660.1 | 26.457 | 446 | 276 | 13 | 5 | 431 | 8 | 420 | 2.42e-24 | 104 |
| Msa0390150 | AT3G54850.1 | 30.065 | 306 | 179 | 12 | 11 | 316 | 252 | 522 | 2.80e-24 | 106 |
| Msa0390150 | AT3G49810.1 | 24.764 | 424 | 271 | 9 | 6 | 429 | 63 | 438 | 1.89e-22 | 99.8 |
| Msa0390150 | AT5G65920.1 | 24.292 | 424 | 273 | 11 | 6 | 429 | 59 | 434 | 1.03e-21 | 97.4 |
| Msa0390150 | AT5G65920.2 | 24.292 | 424 | 273 | 11 | 6 | 429 | 59 | 434 | 1.03e-21 | 97.4 |
| Msa0390150 | AT1G67530.2 | 26.770 | 452 | 260 | 14 | 13 | 418 | 278 | 704 | 1.87e-21 | 97.8 |
| Msa0390150 | AT1G67530.1 | 26.770 | 452 | 260 | 14 | 13 | 418 | 278 | 704 | 1.87e-21 | 97.8 |
| Msa0390150 | AT1G67530.4 | 26.770 | 452 | 260 | 14 | 13 | 418 | 278 | 704 | 1.87e-21 | 97.8 |
| Msa0390150 | AT1G29340.1 | 25.806 | 434 | 256 | 15 | 5 | 421 | 303 | 687 | 8.34e-20 | 92.8 |
| Msa0390150 | AT5G01830.1 | 26.837 | 313 | 177 | 10 | 4 | 308 | 271 | 539 | 1.50e-19 | 92.0 |
| Msa0390150 | AT1G24330.1 | 25.450 | 444 | 272 | 17 | 13 | 418 | 281 | 703 | 6.58e-18 | 87.0 |
| Msa0390150 | AT1G24330.2 | 25.450 | 444 | 272 | 17 | 13 | 418 | 281 | 703 | 6.58e-18 | 87.0 |
| Msa0390150 | AT1G10560.1 | 26.582 | 316 | 177 | 11 | 11 | 313 | 292 | 565 | 1.83e-17 | 85.5 |
| Msa0390150 | AT1G67530.3 | 48.352 | 91 | 35 | 4 | 13 | 103 | 278 | 356 | 1.95e-17 | 85.1 |
| Msa0390150 | AT1G27910.1 | 42.857 | 105 | 49 | 4 | 13 | 117 | 285 | 378 | 5.37e-17 | 84.0 |
| Msa0390150 | AT4G21350.1 | 25.000 | 404 | 249 | 11 | 11 | 412 | 9 | 360 | 3.90e-16 | 80.1 |
| Msa0390150 | AT3G07360.1 | 27.425 | 299 | 186 | 11 | 11 | 309 | 78 | 345 | 1.00e-14 | 76.3 |
| Msa0390150 | AT3G54790.2 | 43.750 | 80 | 38 | 2 | 5 | 84 | 200 | 272 | 7.05e-14 | 74.3 |
| Msa0390150 | AT3G54790.3 | 43.750 | 80 | 38 | 2 | 5 | 84 | 236 | 308 | 8.73e-14 | 73.9 |
| Msa0390150 | AT3G54790.1 | 43.750 | 80 | 38 | 2 | 5 | 84 | 236 | 308 | 8.73e-14 | 73.9 |
| Msa0390150 | AT1G60190.1 | 24.930 | 357 | 209 | 13 | 13 | 354 | 284 | 596 | 1.11e-13 | 73.6 |
| Msa0390150 | AT2G23140.1 | 38.776 | 98 | 49 | 3 | 11 | 108 | 237 | 323 | 2.26e-13 | 72.8 |
| Msa0390150 | AT2G23140.2 | 38.776 | 98 | 49 | 3 | 11 | 108 | 234 | 320 | 2.34e-13 | 72.8 |
| Msa0390150 | AT2G23140.4 | 38.776 | 98 | 49 | 3 | 11 | 108 | 234 | 320 | 2.34e-13 | 72.8 |
| Msa0390150 | AT2G23140.3 | 38.776 | 98 | 49 | 3 | 11 | 108 | 45 | 131 | 2.61e-13 | 72.4 |
| Msa0390150 | AT2G23140.5 | 38.776 | 98 | 49 | 3 | 11 | 108 | 45 | 131 | 2.61e-13 | 72.4 |
| Msa0390150 | AT3G02840.1 | 24.425 | 348 | 220 | 11 | 83 | 417 | 34 | 351 | 4.61e-12 | 67.8 |
| Msa0390150 | AT1G01660.2 | 40.000 | 75 | 38 | 2 | 11 | 85 | 502 | 569 | 5.54e-12 | 68.2 |
| Msa0390150 | AT1G01660.1 | 40.000 | 75 | 38 | 2 | 11 | 85 | 501 | 568 | 5.78e-12 | 68.2 |
| Msa0390150 | AT1G01680.1 | 40.260 | 77 | 39 | 2 | 11 | 87 | 237 | 306 | 1.53e-11 | 65.5 |
| Msa0390150 | AT2G45920.1 | 40.244 | 82 | 42 | 2 | 6 | 87 | 324 | 398 | 2.87e-11 | 65.5 |
| Msa0390150 | AT5G67340.1 | 32.283 | 127 | 74 | 4 | 5 | 128 | 238 | 355 | 3.96e-11 | 65.9 |
| Msa0390150 | AT5G67340.2 | 32.283 | 127 | 74 | 4 | 5 | 128 | 238 | 355 | 3.96e-11 | 65.9 |
| Msa0390150 | AT5G18340.1 | 27.240 | 279 | 156 | 12 | 5 | 273 | 72 | 313 | 6.21e-11 | 64.7 |
| Msa0390150 | AT1G68940.1 | 28.188 | 149 | 81 | 3 | 11 | 156 | 303 | 428 | 8.53e-11 | 64.7 |
| Msa0390150 | AT1G68940.2 | 28.188 | 149 | 81 | 3 | 11 | 156 | 303 | 428 | 9.48e-11 | 64.7 |
| Msa0390150 | AT1G68940.3 | 28.188 | 149 | 81 | 3 | 11 | 156 | 250 | 375 | 9.92e-11 | 64.7 |
Find 50 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAATCAGAAGCTTCTTTA+AGG | 0.125434 | 3_2:+787635 | None:intergenic |
| TTAATCCAACTCGTCGAATT+TGG | 0.143799 | 3_2:-787097 | Msa0390150:CDS |
| TTGATGCTTTCTTTGATATC+TGG | 0.244295 | 3_2:+787137 | None:intergenic |
| TCAAGTTGATAGTGGAAATA+AGG | 0.246468 | 3_2:-786756 | Msa0390150:CDS |
| TGAGATGATGTTGGTGCTTT+TGG | 0.256388 | 3_2:-786990 | Msa0390150:CDS |
| TGCACCATGAATTCTTCTTA+TGG | 0.299615 | 3_2:-787715 | Msa0390150:CDS |
| TTCATGAGTTGTAGTGAAAT+AGG | 0.314008 | 3_2:+787916 | None:intergenic |
| ATGCCTGTTGAGACAGTAAC+TGG | 0.320480 | 3_2:+787889 | None:intergenic |
| TATGAAGCAAGCAAATTAAA+AGG | 0.320835 | 3_2:+786668 | None:intergenic |
| TTAATGAGATTATTGCAAAA+AGG | 0.328958 | 3_2:-787283 | Msa0390150:CDS |
| GTGGTGTAACTTCGATATCT+TGG | 0.376999 | 3_2:+787950 | None:intergenic |
| TTAAATTGCATTCAAAGTCT+TGG | 0.383883 | 3_2:-786709 | Msa0390150:CDS |
| GATTCCATAAGAAGAATTCA+TGG | 0.398042 | 3_2:+787711 | None:intergenic |
| AGAGTAAAAGCAATTGAATC+AGG | 0.420580 | 3_2:-787067 | Msa0390150:CDS |
| TTCTTCCAAATTCGACGAGT+TGG | 0.421885 | 3_2:+787092 | None:intergenic |
| TCATGAGTTGTAGTGAAATA+GGG | 0.428583 | 3_2:+787917 | None:intergenic |
| TGTTGTTGCTAAGCTTTGTT+TGG | 0.429305 | 3_2:-786783 | Msa0390150:CDS |
| TTCTTCTTGATTGCAAAGAA+AGG | 0.442698 | 3_2:-787021 | Msa0390150:CDS |
| GACCCAGTTACTGTCTCAAC+AGG | 0.461302 | 3_2:-787892 | Msa0390150:CDS |
| TTTGATGATGATGATGTTGA+AGG | 0.466030 | 3_2:-787430 | Msa0390150:CDS |
| GTTCAAGAAATGTTACGAAT+TGG | 0.471240 | 3_2:-786806 | Msa0390150:CDS |
| AATTCATGGTGCACCATGCT+TGG | 0.475436 | 3_2:+787725 | None:intergenic |
| TTGGTTCTTCAAGTTGATAG+TGG | 0.510674 | 3_2:-786764 | Msa0390150:CDS |
| CTGATAATTGAAGATTGTAA+AGG | 0.519073 | 3_2:+787359 | None:intergenic |
| ATGTTATGTCAATGTGCTGA+TGG | 0.528834 | 3_2:-786965 | Msa0390150:CDS |
| AGGGTTTGGATAAGTAAATG+AGG | 0.542957 | 3_2:+787604 | None:intergenic |
| AAATCGACGGTGTATCGAAT+CGG | 0.545134 | 3_2:-787545 | Msa0390150:CDS |
| AGTCTGCGTAGAGTGTGGTT+TGG | 0.546280 | 3_2:+787751 | None:intergenic |
| GAACTCTTAAGCCATGGATG+TGG | 0.556866 | 3_2:-786935 | Msa0390150:CDS |
| ATAAGGCAAAGGAGAAAGCA+AGG | 0.557452 | 3_2:-786739 | Msa0390150:CDS |
| ATTCTTCTTATGGAATCGAA+AGG | 0.560631 | 3_2:-787705 | Msa0390150:CDS |
| AGATTTGAACTCTTAAGCCA+TGG | 0.566041 | 3_2:-786941 | Msa0390150:CDS |
| TTCGATGCTTTCTCTGTCGT+AGG | 0.572900 | 3_2:+787864 | None:intergenic |
| TGATAGTGGAAATAAGGCAA+AGG | 0.584798 | 3_2:-786750 | Msa0390150:CDS |
| CGACGGTGTATCGAATCGGC+CGG | 0.591346 | 3_2:-787541 | Msa0390150:CDS |
| TATTTCACTACAACTCATGA+AGG | 0.596000 | 3_2:-787914 | Msa0390150:CDS |
| CTTGCTTCATACCCAACAAG+TGG | 0.596722 | 3_2:-786656 | Msa0390150:CDS |
| TGCTAAGAACTCAACAGCGC+CGG | 0.596748 | 3_2:+787522 | None:intergenic |
| AATCTTCAATTATCAGAACA+AGG | 0.603602 | 3_2:-787352 | Msa0390150:CDS |
| CAATAGCTAAACCACATCCA+TGG | 0.618916 | 3_2:+786924 | None:intergenic |
| CCGTCGATTTGACTCACTCT+CGG | 0.622821 | 3_2:+787558 | None:intergenic |
| TAAGGCAAAGGAGAAAGCAA+GGG | 0.629025 | 3_2:-786738 | Msa0390150:CDS |
| TACGCAGACTCATCCAAGCA+TGG | 0.641143 | 3_2:-787738 | Msa0390150:CDS |
| GGATGAGTCTGCGTAGAGTG+TGG | 0.651981 | 3_2:+787746 | None:intergenic |
| TTGCTTCATACCCAACAAGT+GGG | 0.652979 | 3_2:-786655 | Msa0390150:CDS |
| AGCACCAACATCATCTCACA+TGG | 0.660985 | 3_2:+786995 | None:intergenic |
| TGCCTGTTGAGACAGTAACT+GGG | 0.667901 | 3_2:+787890 | None:intergenic |
| GAAGCCATGTGAGATGATGT+TGG | 0.682586 | 3_2:-786999 | Msa0390150:CDS |
| CCGAGAGTGAGTCAAATCGA+CGG | 0.693824 | 3_2:-787558 | Msa0390150:CDS |
| TGGCTAATGATAGAGCTGTG+AGG | 0.767301 | 3_2:-786871 | Msa0390150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGTTTTGAGCTTTTTTA+GGG | + | chr3_2:787025-787044 | None:intergenic | 20.0% |
| !! | TTAATGAGATTATTGCAAAA+AGG | - | chr3_2:787324-787343 | Msa0390150:CDS | 20.0% |
| !!! | AATTGTTTTGAGCTTTTTTA+GGG | + | chr3_2:787025-787044 | None:intergenic | 20.0% |
| !! | TTAATGAGATTATTGCAAAA+AGG | - | chr3_2:787324-787343 | Msa0390150:CDS | 20.0% |
| !!! | CAATTGTTTTGAGCTTTTTT+AGG | + | chr3_2:787026-787045 | None:intergenic | 25.0% |
| !!! | TTTGCTTTTGACTTCAAAAT+AGG | - | chr3_2:787201-787220 | Msa0390150:CDS | 25.0% |
| ! | CTGATAATTGAAGATTGTAA+AGG | + | chr3_2:787251-787270 | None:intergenic | 25.0% |
| ! | AATCTTCAATTATCAGAACA+AGG | - | chr3_2:787255-787274 | Msa0390150:CDS | 25.0% |
| ! | ACTCTCTTAAACTTCAAAAA+TGG | - | chr3_2:787285-787304 | Msa0390150:CDS | 25.0% |
| !! | TTGTTTCAAAGAAGATTTTG+AGG | - | chr3_2:787703-787722 | Msa0390150:CDS | 25.0% |
| !! | TGTTTCAAAGAAGATTTTGA+GGG | - | chr3_2:787704-787723 | Msa0390150:CDS | 25.0% |
| ! | TTAAATTGCATTCAAAGTCT+TGG | - | chr3_2:787898-787917 | Msa0390150:CDS | 25.0% |
| ! | TATGAAGCAAGCAAATTAAA+AGG | + | chr3_2:787942-787961 | None:intergenic | 25.0% |
| !!! | CAATTGTTTTGAGCTTTTTT+AGG | + | chr3_2:787026-787045 | None:intergenic | 25.0% |
| !!! | TTTGCTTTTGACTTCAAAAT+AGG | - | chr3_2:787201-787220 | Msa0390150:CDS | 25.0% |
| ! | CTGATAATTGAAGATTGTAA+AGG | + | chr3_2:787251-787270 | None:intergenic | 25.0% |
| ! | AATCTTCAATTATCAGAACA+AGG | - | chr3_2:787255-787274 | Msa0390150:CDS | 25.0% |
| ! | ACTCTCTTAAACTTCAAAAA+TGG | - | chr3_2:787285-787304 | Msa0390150:CDS | 25.0% |
| !! | TTGTTTCAAAGAAGATTTTG+AGG | - | chr3_2:787703-787722 | Msa0390150:CDS | 25.0% |
| !! | TGTTTCAAAGAAGATTTTGA+GGG | - | chr3_2:787704-787723 | Msa0390150:CDS | 25.0% |
| ! | TTAAATTGCATTCAAAGTCT+TGG | - | chr3_2:787898-787917 | Msa0390150:CDS | 25.0% |
| ! | TATGAAGCAAGCAAATTAAA+AGG | + | chr3_2:787942-787961 | None:intergenic | 25.0% |
| TCATGAGTTGTAGTGAAATA+GGG | + | chr3_2:786693-786712 | None:intergenic | 30.0% | |
| TTCATGAGTTGTAGTGAAAT+AGG | + | chr3_2:786694-786713 | None:intergenic | 30.0% | |
| TATTTCACTACAACTCATGA+AGG | - | chr3_2:786693-786712 | Msa0390150:CDS | 30.0% | |
| GATTCCATAAGAAGAATTCA+TGG | + | chr3_2:786899-786918 | None:intergenic | 30.0% | |
| ATTCTTCTTATGGAATCGAA+AGG | - | chr3_2:786902-786921 | Msa0390150:CDS | 30.0% | |
| !! | TTTAAGGAGTTTTTCGATGA+GGG | + | chr3_2:786959-786978 | None:intergenic | 30.0% |
| AAGAATCAGAAGCTTCTTTA+AGG | + | chr3_2:786975-786994 | None:intergenic | 30.0% | |
| !!! | TTTTGAGCTTTTTTAGGGTT+TGG | + | chr3_2:787020-787039 | None:intergenic | 30.0% |
| !! | TTTGATGATGATGATGTTGA+AGG | - | chr3_2:787177-787196 | Msa0390150:CDS | 30.0% |
| !!! | TTTAGATTCGCTAGTTTTGA+TGG | + | chr3_2:787411-787430 | None:intergenic | 30.0% |
| TTGATGCTTTCTTTGATATC+TGG | + | chr3_2:787473-787492 | None:intergenic | 30.0% | |
| AGAGTAAAAGCAATTGAATC+AGG | - | chr3_2:787540-787559 | Msa0390150:CDS | 30.0% | |
| TTCTTCTTGATTGCAAAGAA+AGG | - | chr3_2:787586-787605 | Msa0390150:CDS | 30.0% | |
| GTTCAAGAAATGTTACGAAT+TGG | - | chr3_2:787801-787820 | Msa0390150:CDS | 30.0% | |
| !! | TCAAGTTGATAGTGGAAATA+AGG | - | chr3_2:787851-787870 | Msa0390150:CDS | 30.0% |
| TCATGAGTTGTAGTGAAATA+GGG | + | chr3_2:786693-786712 | None:intergenic | 30.0% | |
| TTCATGAGTTGTAGTGAAAT+AGG | + | chr3_2:786694-786713 | None:intergenic | 30.0% | |
| TATTTCACTACAACTCATGA+AGG | - | chr3_2:786693-786712 | Msa0390150:CDS | 30.0% | |
| GATTCCATAAGAAGAATTCA+TGG | + | chr3_2:786899-786918 | None:intergenic | 30.0% | |
| ATTCTTCTTATGGAATCGAA+AGG | - | chr3_2:786902-786921 | Msa0390150:CDS | 30.0% | |
| !! | TTTAAGGAGTTTTTCGATGA+GGG | + | chr3_2:786959-786978 | None:intergenic | 30.0% |
| AAGAATCAGAAGCTTCTTTA+AGG | + | chr3_2:786975-786994 | None:intergenic | 30.0% | |
| !!! | TTTTGAGCTTTTTTAGGGTT+TGG | + | chr3_2:787020-787039 | None:intergenic | 30.0% |
| !! | TTTGATGATGATGATGTTGA+AGG | - | chr3_2:787177-787196 | Msa0390150:CDS | 30.0% |
| !!! | TTTAGATTCGCTAGTTTTGA+TGG | + | chr3_2:787411-787430 | None:intergenic | 30.0% |
| TTGATGCTTTCTTTGATATC+TGG | + | chr3_2:787473-787492 | None:intergenic | 30.0% | |
| AGAGTAAAAGCAATTGAATC+AGG | - | chr3_2:787540-787559 | Msa0390150:CDS | 30.0% | |
| TTCTTCTTGATTGCAAAGAA+AGG | - | chr3_2:787586-787605 | Msa0390150:CDS | 30.0% | |
| GTTCAAGAAATGTTACGAAT+TGG | - | chr3_2:787801-787820 | Msa0390150:CDS | 30.0% | |
| !! | TCAAGTTGATAGTGGAAATA+AGG | - | chr3_2:787851-787870 | Msa0390150:CDS | 30.0% |
| !! | ACAGAGAAAGCATCGAAAAA+TGG | - | chr3_2:786749-786768 | Msa0390150:CDS | 35.0% |
| ! | CGAAAAATGGCTCTTTTCAT+CGG | - | chr3_2:786762-786781 | Msa0390150:CDS | 35.0% |
| TGCACCATGAATTCTTCTTA+TGG | - | chr3_2:786892-786911 | Msa0390150:CDS | 35.0% | |
| !! | TTTTTCGATGAGGGTTTTTG+TGG | + | chr3_2:786950-786969 | None:intergenic | 35.0% |
| !! | CTTTAAGGAGTTTTTCGATG+AGG | + | chr3_2:786960-786979 | None:intergenic | 35.0% |
| AGGGTTTGGATAAGTAAATG+AGG | + | chr3_2:787006-787025 | None:intergenic | 35.0% | |
| AACTAACTCGACGAAAAACT+TGG | + | chr3_2:787436-787455 | None:intergenic | 35.0% | |
| TTAATCCAACTCGTCGAATT+TGG | - | chr3_2:787510-787529 | Msa0390150:CDS | 35.0% | |
| ATGTTATGTCAATGTGCTGA+TGG | - | chr3_2:787642-787661 | Msa0390150:CDS | 35.0% | |
| AGATTTGAACTCTTAAGCCA+TGG | - | chr3_2:787666-787685 | Msa0390150:CDS | 35.0% | |
| !! | GATTTTGAGGGTTTCAACAA+TGG | - | chr3_2:787716-787735 | Msa0390150:CDS | 35.0% |
| !! | TGTTGTTGCTAAGCTTTGTT+TGG | - | chr3_2:787824-787843 | Msa0390150:CDS | 35.0% |
| !! | TTGGTTCTTCAAGTTGATAG+TGG | - | chr3_2:787843-787862 | Msa0390150:CDS | 35.0% |
| !! | TGATAGTGGAAATAAGGCAA+AGG | - | chr3_2:787857-787876 | Msa0390150:CDS | 35.0% |
| !! | ACAGAGAAAGCATCGAAAAA+TGG | - | chr3_2:786749-786768 | Msa0390150:CDS | 35.0% |
| ! | CGAAAAATGGCTCTTTTCAT+CGG | - | chr3_2:786762-786781 | Msa0390150:CDS | 35.0% |
| TGCACCATGAATTCTTCTTA+TGG | - | chr3_2:786892-786911 | Msa0390150:CDS | 35.0% | |
| !! | TTTTTCGATGAGGGTTTTTG+TGG | + | chr3_2:786950-786969 | None:intergenic | 35.0% |
| !! | CTTTAAGGAGTTTTTCGATG+AGG | + | chr3_2:786960-786979 | None:intergenic | 35.0% |
| AGGGTTTGGATAAGTAAATG+AGG | + | chr3_2:787006-787025 | None:intergenic | 35.0% | |
| AACTAACTCGACGAAAAACT+TGG | + | chr3_2:787436-787455 | None:intergenic | 35.0% | |
| TTAATCCAACTCGTCGAATT+TGG | - | chr3_2:787510-787529 | Msa0390150:CDS | 35.0% | |
| ATGTTATGTCAATGTGCTGA+TGG | - | chr3_2:787642-787661 | Msa0390150:CDS | 35.0% | |
| AGATTTGAACTCTTAAGCCA+TGG | - | chr3_2:787666-787685 | Msa0390150:CDS | 35.0% | |
| !! | GATTTTGAGGGTTTCAACAA+TGG | - | chr3_2:787716-787735 | Msa0390150:CDS | 35.0% |
| !! | TGTTGTTGCTAAGCTTTGTT+TGG | - | chr3_2:787824-787843 | Msa0390150:CDS | 35.0% |
| !! | TTGGTTCTTCAAGTTGATAG+TGG | - | chr3_2:787843-787862 | Msa0390150:CDS | 35.0% |
| !! | TGATAGTGGAAATAAGGCAA+AGG | - | chr3_2:787857-787876 | Msa0390150:CDS | 35.0% |
| GTGGTGTAACTTCGATATCT+TGG | + | chr3_2:786660-786679 | None:intergenic | 40.0% | |
| GAAATAGGGCACAGAAAAAG+TGG | + | chr3_2:786679-786698 | None:intergenic | 40.0% | |
| !!! | GTGAGTTGTTGTTTTGTGAC+AGG | + | chr3_2:786802-786821 | None:intergenic | 40.0% |
| ! | GTTTTTGTGGTTGGAGGTTT+TGG | + | chr3_2:786937-786956 | None:intergenic | 40.0% |
| AAATCGACGGTGTATCGAAT+CGG | - | chr3_2:787062-787081 | Msa0390150:CDS | 40.0% | |
| TTCTTCCAAATTCGACGAGT+TGG | + | chr3_2:787518-787537 | None:intergenic | 40.0% | |
| !! | TGAGATGATGTTGGTGCTTT+TGG | - | chr3_2:787617-787636 | Msa0390150:CDS | 40.0% |
| CAATAGCTAAACCACATCCA+TGG | + | chr3_2:787686-787705 | None:intergenic | 40.0% | |
| ATAAGGCAAAGGAGAAAGCA+AGG | - | chr3_2:787868-787887 | Msa0390150:CDS | 40.0% | |
| TAAGGCAAAGGAGAAAGCAA+GGG | - | chr3_2:787869-787888 | Msa0390150:CDS | 40.0% | |
| TTGCTTCATACCCAACAAGT+GGG | - | chr3_2:787952-787971 | Msa0390150:CDS | 40.0% | |
| GTGGTGTAACTTCGATATCT+TGG | + | chr3_2:786660-786679 | None:intergenic | 40.0% | |
| GAAATAGGGCACAGAAAAAG+TGG | + | chr3_2:786679-786698 | None:intergenic | 40.0% | |
| !!! | GTGAGTTGTTGTTTTGTGAC+AGG | + | chr3_2:786802-786821 | None:intergenic | 40.0% |
| ! | GTTTTTGTGGTTGGAGGTTT+TGG | + | chr3_2:786937-786956 | None:intergenic | 40.0% |
| AAATCGACGGTGTATCGAAT+CGG | - | chr3_2:787062-787081 | Msa0390150:CDS | 40.0% | |
| TTCTTCCAAATTCGACGAGT+TGG | + | chr3_2:787518-787537 | None:intergenic | 40.0% | |
| !! | TGAGATGATGTTGGTGCTTT+TGG | - | chr3_2:787617-787636 | Msa0390150:CDS | 40.0% |
| CAATAGCTAAACCACATCCA+TGG | + | chr3_2:787686-787705 | None:intergenic | 40.0% | |
| ATAAGGCAAAGGAGAAAGCA+AGG | - | chr3_2:787868-787887 | Msa0390150:CDS | 40.0% | |
| TAAGGCAAAGGAGAAAGCAA+GGG | - | chr3_2:787869-787888 | Msa0390150:CDS | 40.0% | |
| TTGCTTCATACCCAACAAGT+GGG | - | chr3_2:787952-787971 | Msa0390150:CDS | 40.0% | |
| TGCCTGTTGAGACAGTAACT+GGG | + | chr3_2:786720-786739 | None:intergenic | 45.0% | |
| ATGCCTGTTGAGACAGTAAC+TGG | + | chr3_2:786721-786740 | None:intergenic | 45.0% | |
| TTCGATGCTTTCTCTGTCGT+AGG | + | chr3_2:786746-786765 | None:intergenic | 45.0% | |
| AATTCATGGTGCACCATGCT+TGG | + | chr3_2:786885-786904 | None:intergenic | 45.0% | |
| ! | ATGAGGGTTTTTGTGGTTGG+AGG | + | chr3_2:786943-786962 | None:intergenic | 45.0% |
| ! | TCGATGAGGGTTTTTGTGGT+TGG | + | chr3_2:786946-786965 | None:intergenic | 45.0% |
| GAAGCCATGTGAGATGATGT+TGG | - | chr3_2:787608-787627 | Msa0390150:CDS | 45.0% | |
| AGCACCAACATCATCTCACA+TGG | + | chr3_2:787615-787634 | None:intergenic | 45.0% | |
| GAACTCTTAAGCCATGGATG+TGG | - | chr3_2:787672-787691 | Msa0390150:CDS | 45.0% | |
| TGGCTAATGATAGAGCTGTG+AGG | - | chr3_2:787736-787755 | Msa0390150:CDS | 45.0% | |
| CTTGCTTCATACCCAACAAG+TGG | - | chr3_2:787951-787970 | Msa0390150:CDS | 45.0% | |
| TGCCTGTTGAGACAGTAACT+GGG | + | chr3_2:786720-786739 | None:intergenic | 45.0% | |
| ATGCCTGTTGAGACAGTAAC+TGG | + | chr3_2:786721-786740 | None:intergenic | 45.0% | |
| TTCGATGCTTTCTCTGTCGT+AGG | + | chr3_2:786746-786765 | None:intergenic | 45.0% | |
| AATTCATGGTGCACCATGCT+TGG | + | chr3_2:786885-786904 | None:intergenic | 45.0% | |
| ! | ATGAGGGTTTTTGTGGTTGG+AGG | + | chr3_2:786943-786962 | None:intergenic | 45.0% |
| ! | TCGATGAGGGTTTTTGTGGT+TGG | + | chr3_2:786946-786965 | None:intergenic | 45.0% |
| GAAGCCATGTGAGATGATGT+TGG | - | chr3_2:787608-787627 | Msa0390150:CDS | 45.0% | |
| AGCACCAACATCATCTCACA+TGG | + | chr3_2:787615-787634 | None:intergenic | 45.0% | |
| GAACTCTTAAGCCATGGATG+TGG | - | chr3_2:787672-787691 | Msa0390150:CDS | 45.0% | |
| TGGCTAATGATAGAGCTGTG+AGG | - | chr3_2:787736-787755 | Msa0390150:CDS | 45.0% | |
| CTTGCTTCATACCCAACAAG+TGG | - | chr3_2:787951-787970 | Msa0390150:CDS | 45.0% | |
| GACCCAGTTACTGTCTCAAC+AGG | - | chr3_2:786715-786734 | Msa0390150:CDS | 50.0% | |
| AGTCTGCGTAGAGTGTGGTT+TGG | + | chr3_2:786859-786878 | None:intergenic | 50.0% | |
| TACGCAGACTCATCCAAGCA+TGG | - | chr3_2:786869-786888 | Msa0390150:CDS | 50.0% | |
| !!! | GTGGTTGGAGGTTTTGGAGT+TGG | + | chr3_2:786931-786950 | None:intergenic | 50.0% |
| !! | CCGTCGATTTGACTCACTCT+CGG | + | chr3_2:787052-787071 | None:intergenic | 50.0% |
| CCGAGAGTGAGTCAAATCGA+CGG | - | chr3_2:787049-787068 | Msa0390150:CDS | 50.0% | |
| TGCTAAGAACTCAACAGCGC+CGG | + | chr3_2:787088-787107 | None:intergenic | 50.0% | |
| GACCCAGTTACTGTCTCAAC+AGG | - | chr3_2:786715-786734 | Msa0390150:CDS | 50.0% | |
| AGTCTGCGTAGAGTGTGGTT+TGG | + | chr3_2:786859-786878 | None:intergenic | 50.0% | |
| TACGCAGACTCATCCAAGCA+TGG | - | chr3_2:786869-786888 | Msa0390150:CDS | 50.0% | |
| !!! | GTGGTTGGAGGTTTTGGAGT+TGG | + | chr3_2:786931-786950 | None:intergenic | 50.0% |
| !! | CCGTCGATTTGACTCACTCT+CGG | + | chr3_2:787052-787071 | None:intergenic | 50.0% |
| CCGAGAGTGAGTCAAATCGA+CGG | - | chr3_2:787049-787068 | Msa0390150:CDS | 50.0% | |
| TGCTAAGAACTCAACAGCGC+CGG | + | chr3_2:787088-787107 | None:intergenic | 50.0% | |
| GGATGAGTCTGCGTAGAGTG+TGG | + | chr3_2:786864-786883 | None:intergenic | 55.0% | |
| GGATGAGTCTGCGTAGAGTG+TGG | + | chr3_2:786864-786883 | None:intergenic | 55.0% | |
| CGACGGTGTATCGAATCGGC+CGG | - | chr3_2:787066-787085 | Msa0390150:CDS | 60.0% | |
| CGACGGTGTATCGAATCGGC+CGG | - | chr3_2:787066-787085 | Msa0390150:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 786652 | 787977 | 786652 | ID=Msa0390150;Name=Msa0390150 |
| chr3_2 | mRNA | 786652 | 787977 | 786652 | ID=Msa0390150-mRNA-1;Parent=Msa0390150;Name=Msa0390150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|441 |
| chr3_2 | exon | 786652 | 787977 | 786652 | ID=Msa0390150-mRNA-1:exon:232;Parent=Msa0390150-mRNA-1 |
| chr3_2 | CDS | 786652 | 787977 | 786652 | ID=Msa0390150-mRNA-1:cds;Parent=Msa0390150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
