AlfalfaGEDB | Msa0391000

M. sativa cultivar zhongmu-4 / Msa0391000

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009889 GO:0009909 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2001141
PFAM: PF06203.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0391000	XP_003597300.2	95.219	251	8	2	1	247	1	251	5.89e-172	484

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0391000	Q2HU26	95.219	251	8	2	1	247	1	251	2.81e-172	484

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TFs/TRs:

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Gene ID	Type	Classification
Msa0391000	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0222650	Msa0391000	0.882556	9.436193e-71	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0391000	MtrunA17_Chr2g0327621	95.219	251	8	2	1	247	1	251	5.42e-176	484
Msa0391000	MtrunA17_Chr4g0030421	65.909	132	43	2	118	247	1	132	1.12e-49	159
Msa0391000	MtrunA17_Chr8g0387241	48.101	158	62	7	55	202	132	279	8.72e-29	110
Msa0391000	MtrunA17_Chr5g0397361	43.860	114	43	2	87	200	130	222	4.24e-21	89.0
Msa0391000	MtrunA17_Chr1g0186171	46.000	100	47	2	102	196	264	361	3.70e-20	88.6
Msa0391000	MtrunA17_Chr2g0311591	74.074	54	14	0	142	195	161	214	4.70e-20	86.7
Msa0391000	MtrunA17_Chr1g0168401	43.878	98	51	1	103	196	267	364	3.30e-19	85.9
Msa0391000	MtrunA17_Chr4g0030411	51.807	83	36	1	33	115	29	107	1.66e-17	76.3
Msa0391000	MtrunA17_Chr1g0147111	32.867	143	79	4	62	199	150	280	2.28e-16	76.6
Msa0391000	MtrunA17_Chr3g0126721	57.692	52	22	0	147	198	226	277	4.30e-15	73.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0391000	AT5G59990.1	46.951	164	69	3	66	229	91	236	1.09e-32	119
Msa0391000	AT5G41380.1	42.697	178	73	7	72	243	151	305	1.53e-30	115
Msa0391000	AT1G63820.1	47.581	124	56	2	103	221	139	258	7.61e-28	108
Msa0391000	AT1G04500.3	35.632	174	81	5	34	196	171	324	4.20e-24	99.8
Msa0391000	AT1G04500.4	35.632	174	81	5	34	196	180	333	4.26e-24	99.8
Msa0391000	AT1G04500.1	35.632	174	81	5	34	196	180	333	4.26e-24	99.8
Msa0391000	AT2G33350.4	46.535	101	47	2	103	196	244	344	2.20e-23	97.8
Msa0391000	AT2G33350.2	46.535	101	47	2	103	196	255	355	2.42e-23	97.8
Msa0391000	AT2G33350.5	46.535	101	47	2	103	196	264	364	3.24e-23	97.4
Msa0391000	AT2G33350.1	46.078	102	47	3	103	196	255	356	3.97e-22	94.4

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Find 62 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGATTTGCAGCAACTA+TGG	0.316149	3_2:-2242319	None:intergenic
AAGATTGGAGTACTTGGAGA+AGG	0.605160	3_2:-2240440	None:intergenic
AATAACTCGGAGTTGAGCAG+TGG	0.681830	3_2:+2240395	Msa0391000:CDS
AATGAAATGTCAAGTGTGTA+TGG	0.491009	3_2:-2240174	None:intergenic
ACAGAAGAAAGAGGAATCAG+AGG	0.553930	3_2:+2241145	Msa0391000:CDS
ACTCAGACGATGCTCCGGTG+AGG	0.675434	3_2:+2240552	Msa0391000:CDS
AGAAGAAAGGGTGTTGAGAA+TGG	0.469563	3_2:-2240293	None:intergenic
AGAAGGAGATGGAAGAAGAA+AGG	0.460726	3_2:-2240306	None:intergenic
AGCAGTGGCTATAGTAGCTA+TGG	0.535103	3_2:+2240410	Msa0391000:CDS
AGGATGAAGAAGATGAAAAT+TGG	0.295769	3_2:+2242280	Msa0391000:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAAATGTCAAGTGTGTA+TGG	-	chr3_2:2240177-2240196	None:intergenic	30.0%
TGTTGAGAATGGAATATTGT+TGG	-	chr3_2:2240285-2240304	None:intergenic	30.0%
ATATGTTCCATAAATGGAGA+AGG	-	chr3_2:2240326-2240345	None:intergenic	30.0%
AGTCTAACTCATTGAAATCA+GGG	-	chr3_2:2240349-2240368	None:intergenic	30.0%
ATTCCCTTCAGTACAATAAT+GGG	+	chr3_2:2240486-2240505	Msa0391000:CDS	30.0%
ATACTACTTTCACTAGACAA+TGG	-	chr3_2:2241057-2241076	None:intergenic	30.0%
GAAAGTAGTATGATCATAGA+AGG	+	chr3_2:2241066-2241085	Msa0391000:CDS	30.0%
TTGAAAGATACAGAAGAAAG+AGG	+	chr3_2:2241136-2241155	Msa0391000:CDS	30.0%
CAAAGAGGAAAATAAATGCA+TGG	+	chr3_2:2241373-2241392	Msa0391000:intron	30.0%
CTAAAAGACACTTCAAATCA+TGG	-	chr3_2:2241621-2241640	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	2240125	2242673	2240125	ID=Msa0391000;Name=Msa0391000
chr3_2	mRNA	2240125	2242673	2240125	ID=Msa0391000-mRNA-1;Parent=Msa0391000;Name=Msa0391000-mRNA-1;_AED=0.17;_eAED=0.17;_QI=138\|1\|1\|1\|1\|1\|3\|287\|247
chr3_2	exon	2240125	2240601	2240125	ID=Msa0391000-mRNA-1:exon:715;Parent=Msa0391000-mRNA-1
chr3_2	exon	2241012	2241188	2241012	ID=Msa0391000-mRNA-1:exon:716;Parent=Msa0391000-mRNA-1
chr3_2	exon	2242159	2242673	2242159	ID=Msa0391000-mRNA-1:exon:717;Parent=Msa0391000-mRNA-1
chr3_2	five_prime_UTR	2240125	2240262	2240125	ID=Msa0391000-mRNA-1:five_prime_utr;Parent=Msa0391000-mRNA-1
chr3_2	CDS	2240263	2240601	2240263	ID=Msa0391000-mRNA-1:cds;Parent=Msa0391000-mRNA-1
chr3_2	CDS	2241012	2241188	2241012	ID=Msa0391000-mRNA-1:cds;Parent=Msa0391000-mRNA-1
chr3_2	CDS	2242159	2242386	2242159	ID=Msa0391000-mRNA-1:cds;Parent=Msa0391000-mRNA-1
chr3_2	three_prime_UTR	2242387	2242673	2242387	ID=Msa0391000-mRNA-1:three_prime_utr;Parent=Msa0391000-mRNA-1

Gene Sequence

>Msa0391000

ATGTACGCACACTCTAATTCCAACAATATTCCATTCTCAACACCCTTTCTTCTTCCATCTCCTTCTCCATTTATGGAACATATCCCTGATTTCAATGAGTTAGACTCATTCCAAGACACTCTCTCCCAGCTGAATAACTCGGAGTTGAGCAGTGGCTATAGTAGCTATGGTGGATCACCTTCTCCAAGTACTCCAATCTTGATGCAGAGGAGCATTAGCAGTCATTCCCTTCAGTACAATAATGGGACTCACCATTATCCCCTCTCCGCTTTTTTTGCTGAATTGCTCGACTCAGACGATGCTCCGGTGAGGAAAGTTTGCAGTACCGGCGATTTGCAGAGGATTAACGGAATGCAACATAATCATCATCACGTGGATAGTCCATTGTCTAGTGAAAGTAGTATGATCATAGAAGGTATGAGCAGAGTTTGTCCATATAGCCCAGAAGAAAAGAAAGTGAGAATTGAAAGATACAGAAGAAAGAGGAATCAGAGGAACTTTAACAAGAAAATTAAGTATGTTTGCCGAAAGACATTGGCGGATAGGAGGCCACGAATTAGGGGACGTTTTGCGAGAAATGATGAAATTGATAAGAAACCTATAGTCGAGTGGAGCCACATTGATGGTGGGGAGGAAGAGGATGAAGAAGATGAAAATTGGAATAATATCTTTAATTCCATAGTTGCTGCAAATCTTGTTCATGATGAGTTTCAAGGAAGTTCTTCCTTTGGTCTATTATATTAG

Protein sequence

>Msa0391000

MYAHSNSNNIPFSTPFLLPSPSPFMEHIPDFNELDSFQDTLSQLNNSELSSGYSSYGGSPSPSTPILMQRSISSHSLQYNNGTHHYPLSAFFAELLDSDDAPVRKVCSTGDLQRINGMQHNHHHVDSPLSSESSMIIEGMSRVCPYSPEEKKVRIERYRRKRNQRNFNKKIKYVCRKTLADRRPRIRGRFARNDEIDKKPIVEWSHIDGGEEEDEEDENWNNIFNSIVAANLVHDEFQGSSSFGLLY*