AlfalfaGEDB | Msa0222650

M. sativa cultivar zhongmu-4 / Msa0222650

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009889 GO:0009909 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2001141
PFAM: PF06203.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222650	XP_003597300.2	95.618	251	7	2	1	247	1	251	4.99e-173	486

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222650	Q2HU26	95.618	251	7	2	1	247	1	251	2.38e-173	486

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TFs/TRs:

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Gene ID	Type	Classification
Msa0222650	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0222650	Msa0264190	0.824723	6.914506e-54	-8.615850e-47
Msa0222650	Msa0391000	0.882556	9.436193e-71	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222650	MtrunA17_Chr2g0327621	95.618	251	7	2	1	247	1	251	4.59e-177	486
Msa0222650	MtrunA17_Chr4g0030421	65.909	132	43	2	118	247	1	132	9.32e-50	159
Msa0222650	MtrunA17_Chr8g0387241	48.101	158	62	7	55	202	132	279	8.72e-29	110
Msa0222650	MtrunA17_Chr5g0397361	43.860	114	43	2	87	200	130	222	4.51e-21	89.0
Msa0222650	MtrunA17_Chr1g0186171	46.000	100	47	2	102	196	264	361	3.42e-20	88.6
Msa0222650	MtrunA17_Chr2g0311591	47.059	102	35	2	142	243	161	243	4.84e-20	86.7
Msa0222650	MtrunA17_Chr1g0168401	43.878	98	51	1	103	196	267	364	3.40e-19	85.9
Msa0222650	MtrunA17_Chr4g0030411	51.807	83	36	1	33	115	29	107	1.61e-17	76.3
Msa0222650	MtrunA17_Chr1g0147111	32.867	143	79	4	62	199	150	280	2.37e-16	76.6
Msa0222650	MtrunA17_Chr3g0126721	57.692	52	22	0	147	198	226	277	4.43e-15	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222650	AT5G59990.1	52.899	138	52	2	66	203	91	215	1.70e-32	119
Msa0222650	AT5G41380.1	42.135	178	74	6	72	243	151	305	4.88e-30	114
Msa0222650	AT1G63820.1	47.581	124	56	2	103	221	139	258	8.62e-28	108
Msa0222650	AT1G04500.3	35.632	174	81	5	34	196	171	324	4.69e-24	99.4
Msa0222650	AT1G04500.4	35.632	174	81	5	34	196	180	333	5.06e-24	99.4
Msa0222650	AT1G04500.1	35.632	174	81	5	34	196	180	333	5.06e-24	99.4
Msa0222650	AT2G33350.4	46.535	101	47	2	103	196	244	344	2.56e-23	97.8
Msa0222650	AT2G33350.2	46.535	101	47	2	103	196	255	355	2.79e-23	97.8
Msa0222650	AT2G33350.5	46.535	101	47	2	103	196	264	364	3.65e-23	97.4
Msa0222650	AT2G33350.1	46.078	102	47	3	103	196	255	356	4.56e-22	94.4

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Find 66 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGATTTGCAGCAACTA+TGG	0.316149	2_1:+71933832	None:intergenic
AAGATTGGAGTACTTGGAGA+AGG	0.605160	2_1:+71935703	None:intergenic
AATAACTCGGAGTTGAGCAG+TGG	0.681830	2_1:-71935748	Msa0222650:CDS
AATGAAATGTCAAGTGTGTA+TGG	0.491009	2_1:+71935969	None:intergenic
ACAGAAGAAAGAGGAATCAG+AGG	0.553930	2_1:-71934997	Msa0222650:CDS
ACTCAGACGATGCTCCGGTG+AGG	0.675434	2_1:-71935591	Msa0222650:CDS
AGAAGAAAGGGTGTTGAGAA+TGG	0.469563	2_1:+71935850	None:intergenic
AGAAGGAGATGGAAGAAGAA+AGG	0.460726	2_1:+71935837	None:intergenic
AGCAGTGGCTATAGTAGCTA+TGG	0.535103	2_1:-71935733	Msa0222650:CDS
AGGATGAAGAAGATGAAAAT+TGG	0.295769	2_1:-71933871	Msa0222650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAAATGTCAAGTGTGTA+TGG	+	chr2_1:71933553-71933572	None:intergenic	30.0%
TGTTGAGAATGGAATATTGT+TGG	+	chr2_1:71933661-71933680	None:intergenic	30.0%
ATATGTTCCATAAATGGAGA+AGG	+	chr2_1:71933702-71933721	None:intergenic	30.0%
AGTCTAACTCATTGAAATCA+GGG	+	chr2_1:71933725-71933744	None:intergenic	30.0%
ATTCCCTTCAGTACAATAAT+GGG	-	chr2_1:71933862-71933881	Msa0222650:CDS	30.0%
ATACTACTTTCACTAGACAA+TGG	+	chr2_1:71934434-71934453	None:intergenic	30.0%
GAAAGTAGTATGATCATAGA+AGG	-	chr2_1:71934443-71934462	Msa0222650:intron	30.0%
TTGAAAGATACAGAAGAAAG+AGG	-	chr2_1:71934513-71934532	Msa0222650:intron	30.0%
CAAAGAGGAAAATAAATGCA+TGG	-	chr2_1:71934750-71934769	Msa0222650:intron	30.0%
CTAAAAGACACTTCAAATCA+TGG	+	chr2_1:71934998-71935017	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	71933501	71936040	71933501	ID=Msa0222650;Name=Msa0222650
chr2_1	mRNA	71933501	71936040	71933501	ID=Msa0222650-mRNA-1;Parent=Msa0222650;Name=Msa0222650-mRNA-1;_AED=0.17;_eAED=0.17;_QI=138\|1\|1\|1\|1\|1\|3\|286\|247
chr2_1	exon	71933501	71934014	71933501	ID=Msa0222650-mRNA-1:exon:20761;Parent=Msa0222650-mRNA-1
chr2_1	exon	71934976	71935152	71934976	ID=Msa0222650-mRNA-1:exon:20760;Parent=Msa0222650-mRNA-1
chr2_1	exon	71935564	71936040	71935564	ID=Msa0222650-mRNA-1:exon:20759;Parent=Msa0222650-mRNA-1
chr2_1	five_prime_UTR	71935903	71936040	71935903	ID=Msa0222650-mRNA-1:five_prime_utr;Parent=Msa0222650-mRNA-1
chr2_1	CDS	71935564	71935902	71935564	ID=Msa0222650-mRNA-1:cds;Parent=Msa0222650-mRNA-1
chr2_1	CDS	71934976	71935152	71934976	ID=Msa0222650-mRNA-1:cds;Parent=Msa0222650-mRNA-1
chr2_1	CDS	71933787	71934014	71933787	ID=Msa0222650-mRNA-1:cds;Parent=Msa0222650-mRNA-1
chr2_1	three_prime_UTR	71933501	71933786	71933501	ID=Msa0222650-mRNA-1:three_prime_utr;Parent=Msa0222650-mRNA-1

Gene Sequence

>Msa0222650

ATGTACGCACACTCTAATTCCAACAATATTCCATTCTCAACACCCTTTCTTCTTCCATCTCCTTCTCCATTTATGGAACATATCCCTGATTTCAATGAGTTAGACTCATTCCAAGACACTCTCTCCCAGCTGAATAACTCGGAGTTGAGCAGTGGCTATAGTAGCTATGGTGGATCACCTTCTCCAAGTACTCCAATCTTGATGCAGAGGAGCATTAGCAGTCATTCCCTTCAGTACAATAATGGGACTCACCATTATCCCCTCTCCGCTTTTTTTGCTGAATTGCTCGACTCAGACGATGCTCCGGTGAGGAAAGTTTGCAGTACCGGCGATTTGCAGAGGATTAACGGAATGCAACATAATCATCATCACGTGGATAGTCCATTGTCTAGTGAAAGTAGTATGATCATAGAAGGTATGAGCAGAGTTTGTCCATATAGCCCAGAAGAGAAGAAAGTGAGAATTGAAAGATACAGAAGAAAGAGGAATCAGAGGAACTTTAACAAGAAAATTAAGTATGTTTGCCGAAAGACATTGGCGGATAGGAGGCCACGAATTAGGGGACGTTTTGCGAGAAATGATGAAATTGATAAGAAACCTATAGTCGAGTGGAGCCACATTGGTGGTGGGGAGGAAGAGGATGAAGAAGATGAAAATTGGAATAATATCTTTAATTCCATAGTTGCTGCAAATCTTGTTCATGATGAGTTTCAAGGAAGTTCTTCCTTTGGTCTATTATATTAG

Protein sequence

>Msa0222650

MYAHSNSNNIPFSTPFLLPSPSPFMEHIPDFNELDSFQDTLSQLNNSELSSGYSSYGGSPSPSTPILMQRSISSHSLQYNNGTHHYPLSAFFAELLDSDDAPVRKVCSTGDLQRINGMQHNHHHVDSPLSSESSMIIEGMSRVCPYSPEEKKVRIERYRRKRNQRNFNKKIKYVCRKTLADRRPRIRGRFARNDEIDKKPIVEWSHIGGGEEEDEEDENWNNIFNSIVAANLVHDEFQGSSSFGLLY*