AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0394290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0394290 MtrunA17_Chr3g0079801 95.652 276 12 0 1 276 1 276 0.0 511
Msa0394290 MtrunA17_Chr3g0079791 95.290 276 13 0 1 276 1 276 0.0 511
Msa0394290 MtrunA17_Chr3g0079831 86.957 276 34 1 1 276 17 290 3.34e-164 466
Msa0394290 MtrunA17_Chr4g0051211 59.615 208 84 0 69 276 8 215 2.06e-94 287
Msa0394290 MtrunA17_Chr2g0292971 52.918 257 116 3 19 272 23 277 3.04e-94 288
Msa0394290 MtrunA17_Chr2g0293121 47.778 270 138 2 4 272 3 270 3.55e-93 285
Msa0394290 MtrunA17_Chr2g0292991 55.072 276 121 3 1 275 8 281 1.92e-91 280
Msa0394290 MtrunA17_Chr4g0051551 52.158 278 127 5 1 275 36 310 2.26e-91 281
Msa0394290 MtrunA17_Chr2g0293021 55.036 278 118 3 1 275 8 281 4.88e-91 279
Msa0394290 MtrunA17_Chr4g0051421 52.518 278 126 3 3 276 7 282 5.72e-89 285
Msa0394290 MtrunA17_Chr4g0051421 53.025 281 122 4 2 276 545 821 7.93e-88 282
Msa0394290 MtrunA17_Chr4g0051411 49.455 275 133 4 2 272 6 278 5.02e-87 269
Msa0394290 MtrunA17_Chr4g0051441 46.326 313 128 4 2 276 58 368 2.93e-85 267
Msa0394290 MtrunA17_Chr4g0051381 48.175 274 139 2 4 276 11 282 6.97e-85 263
Msa0394290 MtrunA17_Chr4g0051541 55.906 254 105 5 23 272 28 278 1.39e-84 263
Msa0394290 MtrunA17_Chr2g0293161 47.445 274 139 4 1 272 5 275 3.43e-84 261
Msa0394290 MtrunA17_Chr2g0293041 49.416 257 126 3 17 272 31 284 7.36e-84 261
Msa0394290 MtrunA17_Chr2g0293191 47.670 279 136 5 1 272 31 306 8.44e-84 261
Msa0394290 MtrunA17_Chr2g0293031 46.209 277 141 5 1 272 9 282 2.95e-83 259
Msa0394290 MtrunA17_Chr4g0051531 48.201 278 136 4 1 272 624 899 1.51e-80 263
Msa0394290 MtrunA17_Chr4g0051531 48.708 271 129 6 11 274 23 290 1.36e-73 244
Msa0394290 MtrunA17_Chr4g0048351 47.482 278 132 4 7 276 22 293 3.13e-79 249
Msa0394290 MtrunA17_Chr4g0028611 45.126 277 146 2 1 276 33 304 2.45e-78 247
Msa0394290 MtrunA17_Chr4g0048311 50.196 255 122 4 24 276 37 288 7.12e-78 245
Msa0394290 MtrunA17_Chr4g0046581 47.308 260 123 3 24 276 31 283 4.71e-74 235
Msa0394290 MtrunA17_Chr6g0457061 46.182 275 136 4 7 276 9 276 4.99e-74 235
Msa0394290 MtrunA17_Chr6g0457031 46.182 275 136 4 7 276 9 276 5.15e-74 235
Msa0394290 MtrunA17_Chr4g0048361 45.487 277 143 3 1 276 7 276 8.22e-74 234
Msa0394290 MtrunA17_Chr2g0288611 51.208 207 101 0 70 276 12 218 1.88e-71 227
Msa0394290 MtrunA17_Chr1g0188271 47.137 227 116 3 54 276 60 286 3.15e-57 192
Msa0394290 MtrunA17_Chr4g0048331 48.101 158 82 0 119 276 2 159 1.08e-54 181
Msa0394290 MtrunA17_Chr2g0320381 76.812 69 12 1 164 232 48 112 5.03e-31 112
Msa0394290 MtrunA17_Chr2g0320371 88.889 45 5 0 122 166 1 45 9.01e-22 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0394290 AT4G20860.1 55.390 269 112 3 14 276 17 283 1.23e-91 281
Msa0394290 AT5G44390.1 52.612 268 121 5 13 275 24 290 1.86e-91 281
Msa0394290 AT5G44360.1 57.303 267 93 7 23 276 30 288 1.20e-90 278
Msa0394290 AT5G44360.2 57.303 267 93 7 23 276 46 304 1.99e-90 278
Msa0394290 AT5G44400.1 52.297 283 123 6 4 275 7 288 2.11e-89 275
Msa0394290 AT2G34790.1 49.291 282 125 5 6 276 12 286 1.33e-87 271
Msa0394290 AT4G20820.1 49.259 270 132 3 8 275 19 285 1.24e-84 263
Msa0394290 AT1G11770.1 46.691 272 142 3 4 274 11 280 4.64e-83 259
Msa0394290 AT1G30760.1 51.908 262 119 5 20 276 29 288 9.25e-83 258
Msa0394290 AT1G30760.2 52.107 261 118 5 20 275 47 305 1.00e-82 258
Msa0394290 AT1G26420.1 49.270 274 128 4 8 275 10 278 1.35e-79 250
Msa0394290 AT4G20840.1 48.819 254 128 2 22 274 34 286 3.28e-79 249
Msa0394290 AT1G26400.1 47.584 269 140 1 8 275 10 278 2.42e-78 246
Msa0394290 AT1G26410.1 55.022 229 99 3 48 275 76 301 6.73e-77 243
Msa0394290 AT1G30740.1 47.191 267 137 3 11 274 14 279 6.88e-77 243
Msa0394290 AT1G30740.2 47.191 267 137 3 11 274 17 282 7.71e-77 243
Msa0394290 AT5G44380.1 51.449 276 124 6 9 275 17 291 5.01e-76 241
Msa0394290 AT5G44380.2 51.449 276 124 6 9 275 57 331 9.54e-76 241
Msa0394290 AT1G01980.1 47.744 266 132 4 15 274 21 285 1.06e-75 240
Msa0394290 AT5G44410.1 47.970 271 139 2 7 275 14 284 1.78e-75 239
Msa0394290 AT1G26380.1 45.714 280 141 4 2 275 4 278 2.19e-75 239
Msa0394290 AT2G34810.1 48.077 260 132 3 15 272 24 282 2.62e-75 239
Msa0394290 AT1G30710.1 45.324 278 146 4 4 276 11 287 4.63e-75 238
Msa0394290 AT1G30700.1 49.804 255 123 3 24 275 26 278 7.11e-75 238
Msa0394290 AT1G34575.1 44.326 282 139 6 4 276 11 283 1.14e-74 237
Msa0394290 AT5G44440.1 45.324 278 146 4 2 275 5 280 3.77e-74 236
Msa0394290 AT1G26390.1 53.917 217 99 1 60 275 62 278 1.13e-73 234
Msa0394290 AT5G44440.2 45.324 278 146 4 2 275 75 350 2.68e-73 235
Msa0394290 AT4G20830.2 47.860 257 132 2 19 274 32 287 3.03e-72 231
Msa0394290 AT4G20830.1 47.860 257 132 2 19 274 32 287 8.44e-72 231
Msa0394290 AT4G20800.1 47.036 253 131 3 24 275 27 277 9.22e-72 229
Msa0394290 AT1G30730.1 45.455 264 141 3 8 269 10 272 1.55e-69 224
Msa0394290 AT1G30720.1 41.603 262 150 3 13 272 16 276 8.96e-62 203

Find 75 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTGGTTTAGAAGGTATT+TGG 0.185112 3_2:+8871589 Msa0394290:three_prime_UTR
GCTCACCTTATAGATGTAAA+TGG 0.201621 3_2:+8871060 Msa0394290:CDS
AATGAGAGTTTCCCTGAATT+AGG 0.214946 3_2:+8871405 Msa0394290:three_prime_UTR
TTGTCTTGCAACCCTAATTC+AGG 0.255332 3_2:-8871417 None:intergenic
TCCATGAACTTTACTTGCTT+TGG 0.270151 3_2:-8870659 None:intergenic
AGAGAGAGTTAAACATTGTT+AGG 0.281253 3_2:-8871347 None:intergenic
GGAGGTAGTGCTACTAGTTT+TGG 0.283804 3_2:+8871135 Msa0394290:CDS
CTGCTTGAATTTCTGATTCA+TGG 0.298655 3_2:-8870435 None:intergenic
CTATTCAGCAAGAGTTCTAT+AGG 0.301114 3_2:-8871504 None:intergenic
CGATGAATCTGCTTGGATTC+AGG 0.307823 3_2:+8870600 Msa0394290:CDS
CTTGATGAATTCAGCCATCT+TGG 0.311842 3_2:-8870385 None:intergenic
CACATCAGTGGAGGAGGATT+TGG 0.323062 3_2:+8870991 Msa0394290:CDS
TCCAAAGCAAGTAAAGTTCA+TGG 0.328871 3_2:+8870658 Msa0394290:CDS
CACATTAGCAATGCTTATAA+TGG 0.340644 3_2:+8871632 Msa0394290:three_prime_UTR
CAATACTTGTGGATACAATA+TGG 0.349912 3_2:-8870344 None:intergenic
ATGAGAGTTTCCCTGAATTA+GGG 0.357011 3_2:+8871406 Msa0394290:three_prime_UTR
TCTAAACCAGCTTCTGGTAT+AGG 0.365053 3_2:-8871579 None:intergenic
ATTCAGGCTGGTGCAACATT+AGG 0.380469 3_2:+8870616 Msa0394290:CDS
ATACCTTCTAAACCAGCTTC+TGG 0.406374 3_2:-8871585 None:intergenic
ATACCAGAAGCTGGTTTAGA+AGG 0.411336 3_2:+8871582 Msa0394290:three_prime_UTR
TTGGTTGCTCCTTGTTCCAA+TGG 0.411604 3_2:-8871228 None:intergenic
CATATTGTATCCACAAGTAT+TGG 0.430537 3_2:+8870345 Msa0394290:CDS
GATGAGATCAATCATGATAA+AGG 0.434881 3_2:-8870548 None:intergenic
CTATGTATTGCCACCTATGA+AGG 0.438010 3_2:-8871254 None:intergenic
ATGTTTAACTCTCTCTTCCT+TGG 0.442396 3_2:+8871354 Msa0394290:three_prime_UTR
TTGTTCCAATGGTCTTTGAA+TGG 0.449098 3_2:-8871217 None:intergenic
AGAATAATTGCTCAAAATAG+TGG 0.449896 3_2:+8871306 Msa0394290:three_prime_UTR
GAGCAACCAAGCTCCTTCAT+AGG 0.458493 3_2:+8871241 Msa0394290:CDS
TCATTCTTGCATGGAAGATA+AGG 0.459271 3_2:+8871160 Msa0394290:CDS
CTCACCTTATAGATGTAAAT+GGG 0.461407 3_2:+8871061 Msa0394290:CDS
AATTCTTGATAGAAAATCAA+TGG 0.470027 3_2:+8871086 Msa0394290:CDS
TGTCTTGCAACCCTAATTCA+GGG 0.475992 3_2:-8871416 None:intergenic
AAGACTGCATTGAAATGAGT+TGG 0.478707 3_2:+8871439 Msa0394290:three_prime_UTR
ATGAGATCAATCATGATAAA+GGG 0.478729 3_2:-8870547 None:intergenic
TATTCAGCAAGAGTTCTATA+GGG 0.484064 3_2:-8871503 None:intergenic
ATATGTCCAAGTGTTGGAAT+AGG 0.487241 3_2:+8870964 Msa0394290:CDS
TTCAGTGATTCCAATACTTG+TGG 0.491607 3_2:-8870355 None:intergenic
TTCAGAAAAGCTACTACCTA+TGG 0.493468 3_2:-8870287 None:intergenic
TGCTTGAATTTCTGATTCAT+GGG 0.498893 3_2:-8870434 None:intergenic
AAGACAGTCCTTCATAATCA+TGG 0.503011 3_2:-8870510 None:intergenic
ATCTCGCCGATGAATCTGCT+TGG 0.506621 3_2:+8870593 Msa0394290:CDS
ATTCTTGATAGAAAATCAAT+GGG 0.522100 3_2:+8871087 Msa0394290:CDS
GAGGATTTGGTACCTTGGTA+AGG 0.527609 3_2:+8871004 Msa0394290:CDS
TGACCACCTATTCCAACACT+TGG 0.531376 3_2:-8870970 None:intergenic
AAAGTTCATGGATTCCCTGC+AGG 0.538278 3_2:+8870670 Msa0394290:CDS
TCTTGCATGGAAGATAAGGT+TGG 0.542256 3_2:+8871164 Msa0394290:CDS
ATGCAGTGCATGTTAACCAT+AGG 0.544066 3_2:+8870271 Msa0394290:CDS
GTTCACCATTCAAAGACCAT+TGG 0.545435 3_2:+8871212 Msa0394290:CDS
CACTGAAACAAAATCCAAGA+TGG 0.547598 3_2:+8870371 Msa0394290:CDS
TGGAGGAGGATTTGGTACCT+TGG 0.552904 3_2:+8870999 Msa0394290:CDS
ACAGGTATATGTCCAAGTGT+TGG 0.558034 3_2:+8870958 Msa0394290:intron
AAAGAGCCTATACCAGAAGC+TGG 0.561825 3_2:+8871573 Msa0394290:three_prime_UTR
TGAATTTCTGATTCATGGGA+AGG 0.564377 3_2:-8870430 None:intergenic
CTGAATCCAAGCAGATTCAT+CGG 0.565211 3_2:-8870599 None:intergenic
GGTGGTCACATCAGTGGAGG+AGG 0.567357 3_2:+8870985 Msa0394290:CDS
GTGCAGATTAGAGTGGTTAG+TGG 0.572020 3_2:+8870484 Msa0394290:CDS
TGTCCAAGTGTTGGAATAGG+TGG 0.575899 3_2:+8870967 Msa0394290:CDS
GAATCTGCTTGGATTCAGGC+TGG 0.577913 3_2:+8870604 Msa0394290:CDS
TTGCCACCTATGAAGGAGCT+TGG 0.579989 3_2:-8871247 None:intergenic
GGTACCTTGGTAAGGAAACA+TGG 0.586233 3_2:+8871012 Msa0394290:CDS
AGTGGTGGCCATGATTATGA+AGG 0.592283 3_2:+8870502 Msa0394290:CDS
AAACTAATTAATTACCTGCA+GGG 0.596070 3_2:-8870684 None:intergenic
GATTGGTGTGCAGATTAGAG+TGG 0.599674 3_2:+8870477 Msa0394290:CDS
GAAGTAGTAACATTAAGAGA+TGG 0.613618 3_2:+8870174 Msa0394290:exon
GGAATAGGTGGTCACATCAG+TGG 0.617717 3_2:+8870979 Msa0394290:CDS
CGAGATTAATGTAGATCGAA+CGG 0.620236 3_2:-8870576 None:intergenic
ATTCAAAGACCATTGGAACA+AGG 0.624781 3_2:+8871219 Msa0394290:CDS
GTGAACACTGTAACAGTAGG+TGG 0.627643 3_2:-8871195 None:intergenic
CAACCAAGCTCCTTCATAGG+TGG 0.630946 3_2:+8871244 Msa0394290:CDS
GAAGGTGTCATAATGAGAAG+AGG 0.648177 3_2:-8870412 None:intergenic
TAGACCATGTTTCCTTACCA+AGG 0.652475 3_2:-8871016 None:intergenic
TAAGGTGAGCATCAACAACA+TGG 0.661883 3_2:-8871047 None:intergenic
ATAGGTGGTCACATCAGTGG+AGG 0.667986 3_2:+8870982 Msa0394290:CDS
CAGATTAGAGTGGTTAGTGG+TGG 0.684127 3_2:+8870487 Msa0394290:CDS
ATGGTGAACACTGTAACAGT+AGG 0.736279 3_2:-8871198 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAACAAGTGATATTTTTT+TGG - chr3_2:8870140-8870159 None:intergenic 15.0%
!!! TATAACAAGTGATATTTTTT+TGG - chr3_2:8870140-8870159 None:intergenic 15.0%
!!! ATTTTTTTGGTTCTAACAAA+TGG - chr3_2:8870127-8870146 None:intergenic 20.0%
!! TTGTTATAACCAAAAAGTTA+GGG + chr3_2:8870152-8870171 Msa0394290:five_prime_UTR 20.0%
!! ATTAAATTACTTATCCAAAC+AGG + chr3_2:8870940-8870959 Msa0394290:intron 20.0%
!! AATTCTTGATAGAAAATCAA+TGG + chr3_2:8871086-8871105 Msa0394290:CDS 20.0%
!! ATTCTTGATAGAAAATCAAT+GGG + chr3_2:8871087-8871106 Msa0394290:CDS 20.0%
!!! ATTTTTTTGGTTCTAACAAA+TGG - chr3_2:8870127-8870146 None:intergenic 20.0%
!! TTGTTATAACCAAAAAGTTA+GGG + chr3_2:8870152-8870171 Msa0394290:five_prime_UTR 20.0%
!! ATTAAATTACTTATCCAAAC+AGG + chr3_2:8870940-8870959 Msa0394290:intron 20.0%
!! AATTCTTGATAGAAAATCAA+TGG + chr3_2:8871086-8871105 Msa0394290:CDS 20.0%
!! ATTCTTGATAGAAAATCAAT+GGG + chr3_2:8871087-8871106 Msa0394290:CDS 20.0%
!! CTTGTTATAACCAAAAAGTT+AGG + chr3_2:8870151-8870170 Msa0394290:five_prime_UTR 25.0%
! ATGAGATCAATCATGATAAA+GGG - chr3_2:8870550-8870569 None:intergenic 25.0%
! AAACTAATTAATTACCTGCA+GGG - chr3_2:8870687-8870706 None:intergenic 25.0%
! GCTTACACAATTATACTAAT+AGG - chr3_2:8870726-8870745 None:intergenic 25.0%
!! TTTTCCCATTTACATCTATA+AGG - chr3_2:8871068-8871087 None:intergenic 25.0%
! AGAATAATTGCTCAAAATAG+TGG + chr3_2:8871306-8871325 Msa0394290:three_prime_UTR 25.0%
!! TTAAGTTTTCTTTTTCTCCA+AGG - chr3_2:8871374-8871393 None:intergenic 25.0%
!! CTTGTTATAACCAAAAAGTT+AGG + chr3_2:8870151-8870170 Msa0394290:five_prime_UTR 25.0%
! ATGAGATCAATCATGATAAA+GGG - chr3_2:8870550-8870569 None:intergenic 25.0%
! AAACTAATTAATTACCTGCA+GGG - chr3_2:8870687-8870706 None:intergenic 25.0%
! GCTTACACAATTATACTAAT+AGG - chr3_2:8870726-8870745 None:intergenic 25.0%
!! TTTTCCCATTTACATCTATA+AGG - chr3_2:8871068-8871087 None:intergenic 25.0%
! AGAATAATTGCTCAAAATAG+TGG + chr3_2:8871306-8871325 Msa0394290:three_prime_UTR 25.0%
!! TTAAGTTTTCTTTTTCTCCA+AGG - chr3_2:8871374-8871393 None:intergenic 25.0%
! TTACTACTTCCCTAACTTTT+TGG - chr3_2:8870164-8870183 None:intergenic 30.0%
GAAGTAGTAACATTAAGAGA+TGG + chr3_2:8870174-8870193 Msa0394290:exon 30.0%
CAATACTTGTGGATACAATA+TGG - chr3_2:8870347-8870366 None:intergenic 30.0%
CATATTGTATCCACAAGTAT+TGG + chr3_2:8870345-8870364 Msa0394290:CDS 30.0%
TGCTTGAATTTCTGATTCAT+GGG - chr3_2:8870437-8870456 None:intergenic 30.0%
! AATCTTTTTGCTGCATAGTA+TGG - chr3_2:8870461-8870480 None:intergenic 30.0%
ATACTATGCAGCAAAAAGAT+TGG + chr3_2:8870460-8870479 Msa0394290:CDS 30.0%
GATGAGATCAATCATGATAA+AGG - chr3_2:8870551-8870570 None:intergenic 30.0%
CAAACTAATTAATTACCTGC+AGG - chr3_2:8870688-8870707 None:intergenic 30.0%
CTCACCTTATAGATGTAAAT+GGG + chr3_2:8871061-8871080 Msa0394290:CDS 30.0%
!!! AATGGGAGAAGATGTTTTTT+GGG + chr3_2:8871104-8871123 Msa0394290:CDS 30.0%
AGAGAGAGTTAAACATTGTT+AGG - chr3_2:8871350-8871369 None:intergenic 30.0%
!! CAATCAGTTTTGTATTTTGC+AGG + chr3_2:8871465-8871484 Msa0394290:three_prime_UTR 30.0%
TATTCAGCAAGAGTTCTATA+GGG - chr3_2:8871506-8871525 None:intergenic 30.0%
! CTCTTTTACATAGTCAGATT+TGG - chr3_2:8871559-8871578 None:intergenic 30.0%
CACATTAGCAATGCTTATAA+TGG + chr3_2:8871632-8871651 Msa0394290:three_prime_UTR 30.0%
! TTACTACTTCCCTAACTTTT+TGG - chr3_2:8870164-8870183 None:intergenic 30.0%
GAAGTAGTAACATTAAGAGA+TGG + chr3_2:8870174-8870193 Msa0394290:exon 30.0%
CAATACTTGTGGATACAATA+TGG - chr3_2:8870347-8870366 None:intergenic 30.0%
CATATTGTATCCACAAGTAT+TGG + chr3_2:8870345-8870364 Msa0394290:CDS 30.0%
TGCTTGAATTTCTGATTCAT+GGG - chr3_2:8870437-8870456 None:intergenic 30.0%
! AATCTTTTTGCTGCATAGTA+TGG - chr3_2:8870461-8870480 None:intergenic 30.0%
ATACTATGCAGCAAAAAGAT+TGG + chr3_2:8870460-8870479 Msa0394290:CDS 30.0%
GATGAGATCAATCATGATAA+AGG - chr3_2:8870551-8870570 None:intergenic 30.0%
CAAACTAATTAATTACCTGC+AGG - chr3_2:8870688-8870707 None:intergenic 30.0%
CTCACCTTATAGATGTAAAT+GGG + chr3_2:8871061-8871080 Msa0394290:CDS 30.0%
!!! AATGGGAGAAGATGTTTTTT+GGG + chr3_2:8871104-8871123 Msa0394290:CDS 30.0%
AGAGAGAGTTAAACATTGTT+AGG - chr3_2:8871350-8871369 None:intergenic 30.0%
!! CAATCAGTTTTGTATTTTGC+AGG + chr3_2:8871465-8871484 Msa0394290:three_prime_UTR 30.0%
TATTCAGCAAGAGTTCTATA+GGG - chr3_2:8871506-8871525 None:intergenic 30.0%
! CTCTTTTACATAGTCAGATT+TGG - chr3_2:8871559-8871578 None:intergenic 30.0%
CACATTAGCAATGCTTATAA+TGG + chr3_2:8871632-8871651 Msa0394290:three_prime_UTR 30.0%
TTCAGAAAAGCTACTACCTA+TGG - chr3_2:8870290-8870309 None:intergenic 35.0%
! GATAAACACTGTGTTTTCAC+TGG - chr3_2:8870314-8870333 None:intergenic 35.0%
TTCAGTGATTCCAATACTTG+TGG - chr3_2:8870358-8870377 None:intergenic 35.0%
CACTGAAACAAAATCCAAGA+TGG + chr3_2:8870371-8870390 Msa0394290:CDS 35.0%
TGAATTTCTGATTCATGGGA+AGG - chr3_2:8870433-8870452 None:intergenic 35.0%
CTGCTTGAATTTCTGATTCA+TGG - chr3_2:8870438-8870457 None:intergenic 35.0%
AAGACAGTCCTTCATAATCA+TGG - chr3_2:8870513-8870532 None:intergenic 35.0%
CGAGATTAATGTAGATCGAA+CGG - chr3_2:8870579-8870598 None:intergenic 35.0%
TCCAAAGCAAGTAAAGTTCA+TGG + chr3_2:8870658-8870677 Msa0394290:CDS 35.0%
TCCATGAACTTTACTTGCTT+TGG - chr3_2:8870662-8870681 None:intergenic 35.0%
!! CTTGTTTCTATAAGCTCTCA+AGG + chr3_2:8870811-8870830 Msa0394290:intron 35.0%
!! ATATGTCCAAGTGTTGGAAT+AGG + chr3_2:8870964-8870983 Msa0394290:CDS 35.0%
GCTCACCTTATAGATGTAAA+TGG + chr3_2:8871060-8871079 Msa0394290:CDS 35.0%
!!! CAATGGGAGAAGATGTTTTT+TGG + chr3_2:8871103-8871122 Msa0394290:CDS 35.0%
!!! GATGTTTTTTGGGCTATTAG+AGG + chr3_2:8871114-8871133 Msa0394290:CDS 35.0%
!!! GTTTTGGAATCATTCTTGCA+TGG + chr3_2:8871151-8871170 Msa0394290:CDS 35.0%
TCATTCTTGCATGGAAGATA+AGG + chr3_2:8871160-8871179 Msa0394290:CDS 35.0%
TTGTTCCAATGGTCTTTGAA+TGG - chr3_2:8871220-8871239 None:intergenic 35.0%
ATTCAAAGACCATTGGAACA+AGG + chr3_2:8871219-8871238 Msa0394290:CDS 35.0%
ATGTTTAACTCTCTCTTCCT+TGG + chr3_2:8871354-8871373 Msa0394290:three_prime_UTR 35.0%
AATGAGAGTTTCCCTGAATT+AGG + chr3_2:8871405-8871424 Msa0394290:three_prime_UTR 35.0%
ATGAGAGTTTCCCTGAATTA+GGG + chr3_2:8871406-8871425 Msa0394290:three_prime_UTR 35.0%
AAGACTGCATTGAAATGAGT+TGG + chr3_2:8871439-8871458 Msa0394290:three_prime_UTR 35.0%
CTATTCAGCAAGAGTTCTAT+AGG - chr3_2:8871507-8871526 None:intergenic 35.0%
!! AAGCTGGTTTAGAAGGTATT+TGG + chr3_2:8871589-8871608 Msa0394290:three_prime_UTR 35.0%
TTCAGAAAAGCTACTACCTA+TGG - chr3_2:8870290-8870309 None:intergenic 35.0%
! GATAAACACTGTGTTTTCAC+TGG - chr3_2:8870314-8870333 None:intergenic 35.0%
TTCAGTGATTCCAATACTTG+TGG - chr3_2:8870358-8870377 None:intergenic 35.0%
CACTGAAACAAAATCCAAGA+TGG + chr3_2:8870371-8870390 Msa0394290:CDS 35.0%
TGAATTTCTGATTCATGGGA+AGG - chr3_2:8870433-8870452 None:intergenic 35.0%
CTGCTTGAATTTCTGATTCA+TGG - chr3_2:8870438-8870457 None:intergenic 35.0%
AAGACAGTCCTTCATAATCA+TGG - chr3_2:8870513-8870532 None:intergenic 35.0%
CGAGATTAATGTAGATCGAA+CGG - chr3_2:8870579-8870598 None:intergenic 35.0%
TCCAAAGCAAGTAAAGTTCA+TGG + chr3_2:8870658-8870677 Msa0394290:CDS 35.0%
TCCATGAACTTTACTTGCTT+TGG - chr3_2:8870662-8870681 None:intergenic 35.0%
!! CTTGTTTCTATAAGCTCTCA+AGG + chr3_2:8870811-8870830 Msa0394290:intron 35.0%
!! ATATGTCCAAGTGTTGGAAT+AGG + chr3_2:8870964-8870983 Msa0394290:CDS 35.0%
GCTCACCTTATAGATGTAAA+TGG + chr3_2:8871060-8871079 Msa0394290:CDS 35.0%
!!! CAATGGGAGAAGATGTTTTT+TGG + chr3_2:8871103-8871122 Msa0394290:CDS 35.0%
!!! GATGTTTTTTGGGCTATTAG+AGG + chr3_2:8871114-8871133 Msa0394290:CDS 35.0%
!!! GTTTTGGAATCATTCTTGCA+TGG + chr3_2:8871151-8871170 Msa0394290:CDS 35.0%
TCATTCTTGCATGGAAGATA+AGG + chr3_2:8871160-8871179 Msa0394290:CDS 35.0%
TTGTTCCAATGGTCTTTGAA+TGG - chr3_2:8871220-8871239 None:intergenic 35.0%
ATTCAAAGACCATTGGAACA+AGG + chr3_2:8871219-8871238 Msa0394290:CDS 35.0%
ATGTTTAACTCTCTCTTCCT+TGG + chr3_2:8871354-8871373 Msa0394290:three_prime_UTR 35.0%
AATGAGAGTTTCCCTGAATT+AGG + chr3_2:8871405-8871424 Msa0394290:three_prime_UTR 35.0%
ATGAGAGTTTCCCTGAATTA+GGG + chr3_2:8871406-8871425 Msa0394290:three_prime_UTR 35.0%
AAGACTGCATTGAAATGAGT+TGG + chr3_2:8871439-8871458 Msa0394290:three_prime_UTR 35.0%
CTATTCAGCAAGAGTTCTAT+AGG - chr3_2:8871507-8871526 None:intergenic 35.0%
!! AAGCTGGTTTAGAAGGTATT+TGG + chr3_2:8871589-8871608 Msa0394290:three_prime_UTR 35.0%
ATGCAGTGCATGTTAACCAT+AGG + chr3_2:8870271-8870290 Msa0394290:CDS 40.0%
CTTGATGAATTCAGCCATCT+TGG - chr3_2:8870388-8870407 None:intergenic 40.0%
!! GAAGGTGTCATAATGAGAAG+AGG - chr3_2:8870415-8870434 None:intergenic 40.0%
CTGAATCCAAGCAGATTCAT+CGG - chr3_2:8870602-8870621 None:intergenic 40.0%
CACTTGGACATATACCTGTT+TGG - chr3_2:8870957-8870976 None:intergenic 40.0%
ACAGGTATATGTCCAAGTGT+TGG + chr3_2:8870958-8870977 Msa0394290:intron 40.0%
TAGACCATGTTTCCTTACCA+AGG - chr3_2:8871019-8871038 None:intergenic 40.0%
TAAGGTGAGCATCAACAACA+TGG - chr3_2:8871050-8871069 None:intergenic 40.0%
!!! GTTTTTTGGGCTATTAGAGG+AGG + chr3_2:8871117-8871136 Msa0394290:CDS 40.0%
TCTTGCATGGAAGATAAGGT+TGG + chr3_2:8871164-8871183 Msa0394290:CDS 40.0%
ATGGTGAACACTGTAACAGT+AGG - chr3_2:8871201-8871220 None:intergenic 40.0%
GTTCACCATTCAAAGACCAT+TGG + chr3_2:8871212-8871231 Msa0394290:CDS 40.0%
CTATGTATTGCCACCTATGA+AGG - chr3_2:8871257-8871276 None:intergenic 40.0%
TGTCTTGCAACCCTAATTCA+GGG - chr3_2:8871419-8871438 None:intergenic 40.0%
TTGTCTTGCAACCCTAATTC+AGG - chr3_2:8871420-8871439 None:intergenic 40.0%
!!! GGCTTTGAATGAGCTTTTGA+AGG - chr3_2:8871538-8871557 None:intergenic 40.0%
TCTAAACCAGCTTCTGGTAT+AGG - chr3_2:8871582-8871601 None:intergenic 40.0%
! ATACCAGAAGCTGGTTTAGA+AGG + chr3_2:8871582-8871601 Msa0394290:three_prime_UTR 40.0%
ATACCTTCTAAACCAGCTTC+TGG - chr3_2:8871588-8871607 None:intergenic 40.0%
ATGCAGTGCATGTTAACCAT+AGG + chr3_2:8870271-8870290 Msa0394290:CDS 40.0%
CTTGATGAATTCAGCCATCT+TGG - chr3_2:8870388-8870407 None:intergenic 40.0%
!! GAAGGTGTCATAATGAGAAG+AGG - chr3_2:8870415-8870434 None:intergenic 40.0%
CTGAATCCAAGCAGATTCAT+CGG - chr3_2:8870602-8870621 None:intergenic 40.0%
CACTTGGACATATACCTGTT+TGG - chr3_2:8870957-8870976 None:intergenic 40.0%
ACAGGTATATGTCCAAGTGT+TGG + chr3_2:8870958-8870977 Msa0394290:intron 40.0%
TAGACCATGTTTCCTTACCA+AGG - chr3_2:8871019-8871038 None:intergenic 40.0%
TAAGGTGAGCATCAACAACA+TGG - chr3_2:8871050-8871069 None:intergenic 40.0%
!!! GTTTTTTGGGCTATTAGAGG+AGG + chr3_2:8871117-8871136 Msa0394290:CDS 40.0%
TCTTGCATGGAAGATAAGGT+TGG + chr3_2:8871164-8871183 Msa0394290:CDS 40.0%
ATGGTGAACACTGTAACAGT+AGG - chr3_2:8871201-8871220 None:intergenic 40.0%
GTTCACCATTCAAAGACCAT+TGG + chr3_2:8871212-8871231 Msa0394290:CDS 40.0%
CTATGTATTGCCACCTATGA+AGG - chr3_2:8871257-8871276 None:intergenic 40.0%
TGTCTTGCAACCCTAATTCA+GGG - chr3_2:8871419-8871438 None:intergenic 40.0%
TTGTCTTGCAACCCTAATTC+AGG - chr3_2:8871420-8871439 None:intergenic 40.0%
!!! GGCTTTGAATGAGCTTTTGA+AGG - chr3_2:8871538-8871557 None:intergenic 40.0%
TCTAAACCAGCTTCTGGTAT+AGG - chr3_2:8871582-8871601 None:intergenic 40.0%
! ATACCAGAAGCTGGTTTAGA+AGG + chr3_2:8871582-8871601 Msa0394290:three_prime_UTR 40.0%
ATACCTTCTAAACCAGCTTC+TGG - chr3_2:8871588-8871607 None:intergenic 40.0%
GATTGGTGTGCAGATTAGAG+TGG + chr3_2:8870477-8870496 Msa0394290:CDS 45.0%
GTGCAGATTAGAGTGGTTAG+TGG + chr3_2:8870484-8870503 Msa0394290:CDS 45.0%
CAGATTAGAGTGGTTAGTGG+TGG + chr3_2:8870487-8870506 Msa0394290:CDS 45.0%
AGTGGTGGCCATGATTATGA+AGG + chr3_2:8870502-8870521 Msa0394290:CDS 45.0%
CGATGAATCTGCTTGGATTC+AGG + chr3_2:8870600-8870619 Msa0394290:CDS 45.0%
ATTCAGGCTGGTGCAACATT+AGG + chr3_2:8870616-8870635 Msa0394290:CDS 45.0%
AAAGTTCATGGATTCCCTGC+AGG + chr3_2:8870670-8870689 Msa0394290:CDS 45.0%
!! TGTCCAAGTGTTGGAATAGG+TGG + chr3_2:8870967-8870986 Msa0394290:CDS 45.0%
TGACCACCTATTCCAACACT+TGG - chr3_2:8870973-8870992 None:intergenic 45.0%
! GAGGATTTGGTACCTTGGTA+AGG + chr3_2:8871004-8871023 Msa0394290:CDS 45.0%
GGTACCTTGGTAAGGAAACA+TGG + chr3_2:8871012-8871031 Msa0394290:CDS 45.0%
!! GGAGGTAGTGCTACTAGTTT+TGG + chr3_2:8871135-8871154 Msa0394290:CDS 45.0%
GTGAACACTGTAACAGTAGG+TGG - chr3_2:8871198-8871217 None:intergenic 45.0%
TTGGTTGCTCCTTGTTCCAA+TGG - chr3_2:8871231-8871250 None:intergenic 45.0%
AAAGAGCCTATACCAGAAGC+TGG + chr3_2:8871573-8871592 Msa0394290:three_prime_UTR 45.0%
GATTGGTGTGCAGATTAGAG+TGG + chr3_2:8870477-8870496 Msa0394290:CDS 45.0%
GTGCAGATTAGAGTGGTTAG+TGG + chr3_2:8870484-8870503 Msa0394290:CDS 45.0%
CAGATTAGAGTGGTTAGTGG+TGG + chr3_2:8870487-8870506 Msa0394290:CDS 45.0%
AGTGGTGGCCATGATTATGA+AGG + chr3_2:8870502-8870521 Msa0394290:CDS 45.0%
CGATGAATCTGCTTGGATTC+AGG + chr3_2:8870600-8870619 Msa0394290:CDS 45.0%
ATTCAGGCTGGTGCAACATT+AGG + chr3_2:8870616-8870635 Msa0394290:CDS 45.0%
AAAGTTCATGGATTCCCTGC+AGG + chr3_2:8870670-8870689 Msa0394290:CDS 45.0%
!! TGTCCAAGTGTTGGAATAGG+TGG + chr3_2:8870967-8870986 Msa0394290:CDS 45.0%
TGACCACCTATTCCAACACT+TGG - chr3_2:8870973-8870992 None:intergenic 45.0%
! GAGGATTTGGTACCTTGGTA+AGG + chr3_2:8871004-8871023 Msa0394290:CDS 45.0%
GGTACCTTGGTAAGGAAACA+TGG + chr3_2:8871012-8871031 Msa0394290:CDS 45.0%
!! GGAGGTAGTGCTACTAGTTT+TGG + chr3_2:8871135-8871154 Msa0394290:CDS 45.0%
GTGAACACTGTAACAGTAGG+TGG - chr3_2:8871198-8871217 None:intergenic 45.0%
TTGGTTGCTCCTTGTTCCAA+TGG - chr3_2:8871231-8871250 None:intergenic 45.0%
AAAGAGCCTATACCAGAAGC+TGG + chr3_2:8871573-8871592 Msa0394290:three_prime_UTR 45.0%
ATCTCGCCGATGAATCTGCT+TGG + chr3_2:8870593-8870612 Msa0394290:CDS 50.0%
GAATCTGCTTGGATTCAGGC+TGG + chr3_2:8870604-8870623 Msa0394290:CDS 50.0%
GGAATAGGTGGTCACATCAG+TGG + chr3_2:8870979-8870998 Msa0394290:CDS 50.0%
ATAGGTGGTCACATCAGTGG+AGG + chr3_2:8870982-8871001 Msa0394290:CDS 50.0%
CACATCAGTGGAGGAGGATT+TGG + chr3_2:8870991-8871010 Msa0394290:CDS 50.0%
! TGGAGGAGGATTTGGTACCT+TGG + chr3_2:8870999-8871018 Msa0394290:CDS 50.0%
GAGCAACCAAGCTCCTTCAT+AGG + chr3_2:8871241-8871260 Msa0394290:CDS 50.0%
CAACCAAGCTCCTTCATAGG+TGG + chr3_2:8871244-8871263 Msa0394290:CDS 50.0%
TTGCCACCTATGAAGGAGCT+TGG - chr3_2:8871250-8871269 None:intergenic 50.0%
ATCTCGCCGATGAATCTGCT+TGG + chr3_2:8870593-8870612 Msa0394290:CDS 50.0%
GAATCTGCTTGGATTCAGGC+TGG + chr3_2:8870604-8870623 Msa0394290:CDS 50.0%
GGAATAGGTGGTCACATCAG+TGG + chr3_2:8870979-8870998 Msa0394290:CDS 50.0%
ATAGGTGGTCACATCAGTGG+AGG + chr3_2:8870982-8871001 Msa0394290:CDS 50.0%
CACATCAGTGGAGGAGGATT+TGG + chr3_2:8870991-8871010 Msa0394290:CDS 50.0%
! TGGAGGAGGATTTGGTACCT+TGG + chr3_2:8870999-8871018 Msa0394290:CDS 50.0%
GAGCAACCAAGCTCCTTCAT+AGG + chr3_2:8871241-8871260 Msa0394290:CDS 50.0%
CAACCAAGCTCCTTCATAGG+TGG + chr3_2:8871244-8871263 Msa0394290:CDS 50.0%
TTGCCACCTATGAAGGAGCT+TGG - chr3_2:8871250-8871269 None:intergenic 50.0%
GGTGGTCACATCAGTGGAGG+AGG + chr3_2:8870985-8871004 Msa0394290:CDS 60.0%
GGTGGTCACATCAGTGGAGG+AGG + chr3_2:8870985-8871004 Msa0394290:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 8870123 8871657 8870123 ID=Msa0394290;Name=Msa0394290
chr3_2 mRNA 8870123 8871657 8870123 ID=Msa0394290-mRNA-1;Parent=Msa0394290;Name=Msa0394290-mRNA-1;_AED=0.31;_eAED=0.31;_QI=70|1|1|1|1|1|2|364|276
chr3_2 exon 8870123 8870691 8870123 ID=Msa0394290-mRNA-1:exon:2293;Parent=Msa0394290-mRNA-1
chr3_2 exon 8870962 8871657 8870962 ID=Msa0394290-mRNA-1:exon:2294;Parent=Msa0394290-mRNA-1
chr3_2 five_prime_UTR 8870123 8870192 8870123 ID=Msa0394290-mRNA-1:five_prime_utr;Parent=Msa0394290-mRNA-1
chr3_2 CDS 8870193 8870691 8870193 ID=Msa0394290-mRNA-1:cds;Parent=Msa0394290-mRNA-1
chr3_2 CDS 8870962 8871293 8870962 ID=Msa0394290-mRNA-1:cds;Parent=Msa0394290-mRNA-1
chr3_2 three_prime_UTR 8871294 8871657 8871294 ID=Msa0394290-mRNA-1:three_prime_utr;Parent=Msa0394290-mRNA-1
Gene Sequence

>Msa0394290

ATGGCTTATCTTGCAGTGTTTCTGATTCTATCTCTTTCAATCTCTTGTGCTTCTTCTACTTCAGTTGAGAAAAGTTTCATGCAGTGCATGTTAACCATAGGTAGTAGCTTTTCTGAATCCAGTGAAAACACAGTGTTTATCAACTCATCATCCATATTGTATCCACAAGTATTGGAATCACTGAAACAAAATCCAAGATGGCTGAATTCATCAAGCAAGCCTCTTCTCATTATGACACCTTCCCATGAATCAGAAATTCAAGCAGCCATACTATGCAGCAAAAAGATTGGTGTGCAGATTAGAGTGGTTAGTGGTGGCCATGATTATGAAGGACTGTCTTATCTTTGCAAGACACCCTTTATCATGATTGATCTCATCAACATCCGTTCGATCTACATTAATCTCGCCGATGAATCTGCTTGGATTCAGGCTGGTGCAACATTAGGTGAACTTTACTACAAAATTTCCAAAGCAAGTAAAGTTCATGGATTCCCTGCAGGTATATGTCCAAGTGTTGGAATAGGTGGTCACATCAGTGGAGGAGGATTTGGTACCTTGGTAAGGAAACATGGTCTAGCAGCAGACCATGTTGTTGATGCTCACCTTATAGATGTAAATGGGAAAATTCTTGATAGAAAATCAATGGGAGAAGATGTTTTTTGGGCTATTAGAGGAGGTAGTGCTACTAGTTTTGGAATCATTCTTGCATGGAAGATAAGGTTGGTTAGAGTTCCACCTACTGTTACAGTGTTCACCATTCAAAGACCATTGGAACAAGGAGCAACCAAGCTCCTTCATAGGTGGCAATACATAGCAGATAAATTGCAATAA
Protein sequence

>Msa0394290

MAYLAVFLILSLSISCASSTSVEKSFMQCMLTIGSSFSESSENTVFINSSSILYPQVLESLKQNPRWLNSSSKPLLIMTPSHESEIQAAILCSKKIGVQIRVVSGGHDYEGLSYLCKTPFIMIDLINIRSIYINLADESAWIQAGATLGELYYKISKASKVHGFPAGICPSVGIGGHISGGGFGTLVRKHGLAADHVVDAHLIDVNGKILDRKSMGEDVFWAIRGGSATSFGIILAWKIRLVRVPPTVTVFTIQRPLEQGATKLLHRWQYIADKLQ*