Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399930 | XP_024633996.1 | 86.765 | 68 | 9 | 0 | 1 | 68 | 1 | 68 | 4.70e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399930 | G7IZF3 | 86.765 | 68 | 9 | 0 | 1 | 68 | 1 | 68 | 2.24e-33 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0209360 | Msa0399930 | 0.816979 | 4.181743e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCGTTGGCTCCGGCACC+AGG | 0.351170 | 3_2:-23743489 | None:intergenic |
| ATGATGCCATTGTAAAACTA+TGG | 0.384022 | 3_2:+23743586 | Msa0399930:CDS |
| CCGGCACCAGGTCCTGATGC+TGG | 0.402695 | 3_2:-23743477 | None:intergenic |
| GATCCGGCAGCTCCAGCATC+AGG | 0.425147 | 3_2:+23743465 | Msa0399930:CDS |
| GGTCCTGATGCTGGAGCTGC+CGG | 0.466366 | 3_2:-23743468 | None:intergenic |
| CCGGAGCCAACGAGGGACTA+AGG | 0.496351 | 3_2:+23743496 | Msa0399930:CDS |
| CCAGCATCAGGACCTGGTGC+CGG | 0.497543 | 3_2:+23743477 | Msa0399930:CDS |
| CACTGAACTTGTAACAGATC+CGG | 0.503283 | 3_2:+23743449 | Msa0399930:CDS |
| AAGGTCTTGCGCAGAGGCCA+CGG | 0.519487 | 3_2:+23743515 | Msa0399930:CDS |
| CCTTAGTCCCTCGTTGGCTC+CGG | 0.543980 | 3_2:-23743496 | None:intergenic |
| GCAGCTCCAGCATCAGGACC+TGG | 0.550326 | 3_2:+23743471 | Msa0399930:CDS |
| ACAAGTTCAGTGGCTATGAT+CGG | 0.577020 | 3_2:-23743438 | None:intergenic |
| GCAAGACCTTAGTCCCTCGT+TGG | 0.605286 | 3_2:-23743502 | None:intergenic |
| CGGATCTGTTACAAGTTCAG+TGG | 0.630466 | 3_2:-23743448 | None:intergenic |
| CCTGGTGCCGGAGCCAACGA+GGG | 0.637311 | 3_2:+23743489 | Msa0399930:CDS |
| GGGACTAAGGTCTTGCGCAG+AGG | 0.651443 | 3_2:+23743509 | Msa0399930:CDS |
| ACCTGGTGCCGGAGCCAACG+AGG | 0.655600 | 3_2:+23743488 | Msa0399930:CDS |
| GTCTTGCGCAGAGGCCACGG+TGG | 0.665340 | 3_2:+23743518 | Msa0399930:CDS |
| CGTTATCTTCGTCACCACCG+TGG | 0.725562 | 3_2:-23743532 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTATTCCATAGTTTTACAA+TGG | - | chr3_2:23743595-23743614 | None:intergenic | 25.0% |
| ATGATGCCATTGTAAAACTA+TGG | + | chr3_2:23743586-23743605 | Msa0399930:CDS | 30.0% | |
| GAAGATAACGACGAAAAAGA+AGG | + | chr3_2:23743545-23743564 | Msa0399930:CDS | 35.0% | |
| ACAAGTTCAGTGGCTATGAT+CGG | - | chr3_2:23743441-23743460 | None:intergenic | 40.0% | |
| CACTGAACTTGTAACAGATC+CGG | + | chr3_2:23743449-23743468 | Msa0399930:CDS | 40.0% | |
| CGGATCTGTTACAAGTTCAG+TGG | - | chr3_2:23743451-23743470 | None:intergenic | 45.0% | |
| GCAAGACCTTAGTCCCTCGT+TGG | - | chr3_2:23743505-23743524 | None:intergenic | 55.0% | |
| CGTTATCTTCGTCACCACCG+TGG | - | chr3_2:23743535-23743554 | None:intergenic | 55.0% | |
| !! | CCTTAGTCCCTCGTTGGCTC+CGG | - | chr3_2:23743499-23743518 | None:intergenic | 60.0% |
| GGGACTAAGGTCTTGCGCAG+AGG | + | chr3_2:23743509-23743528 | Msa0399930:CDS | 60.0% | |
| AAGGTCTTGCGCAGAGGCCA+CGG | + | chr3_2:23743515-23743534 | Msa0399930:CDS | 60.0% | |
| GATCCGGCAGCTCCAGCATC+AGG | + | chr3_2:23743465-23743484 | Msa0399930:CDS | 65.0% | |
| GGTCCTGATGCTGGAGCTGC+CGG | - | chr3_2:23743471-23743490 | None:intergenic | 65.0% | |
| GCAGCTCCAGCATCAGGACC+TGG | + | chr3_2:23743471-23743490 | Msa0399930:CDS | 65.0% | |
| CCAGCATCAGGACCTGGTGC+CGG | + | chr3_2:23743477-23743496 | Msa0399930:CDS | 65.0% | |
| CCGGAGCCAACGAGGGACTA+AGG | + | chr3_2:23743496-23743515 | Msa0399930:CDS | 65.0% | |
| CCGGCACCAGGTCCTGATGC+TGG | - | chr3_2:23743480-23743499 | None:intergenic | 70.0% | |
| ! | ACCTGGTGCCGGAGCCAACG+AGG | + | chr3_2:23743488-23743507 | Msa0399930:CDS | 70.0% |
| ! | CCTGGTGCCGGAGCCAACGA+GGG | + | chr3_2:23743489-23743508 | Msa0399930:CDS | 70.0% |
| GTCTTGCGCAGAGGCCACGG+TGG | + | chr3_2:23743518-23743537 | Msa0399930:CDS | 70.0% | |
| !! | CCCTCGTTGGCTCCGGCACC+AGG | - | chr3_2:23743492-23743511 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 23743408 | 23743620 | 23743408 | ID=Msa0399930;Name=Msa0399930 |
| chr3_2 | mRNA | 23743408 | 23743620 | 23743408 | ID=Msa0399930-mRNA-1;Parent=Msa0399930;Name=Msa0399930-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|70 |
| chr3_2 | exon | 23743408 | 23743620 | 23743408 | ID=Msa0399930-mRNA-1:exon:4998;Parent=Msa0399930-mRNA-1 |
| chr3_2 | CDS | 23743408 | 23743620 | 23743408 | ID=Msa0399930-mRNA-1:cds;Parent=Msa0399930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
