AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0400620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0400620 MtrunA17_Chr1g0167311 91.667 144 12 0 1 144 192 335 1.43e-92 277
Msa0400620 MtrunA17_Chr1g0167351 87.143 140 18 0 1 140 187 326 6.59e-85 258
Msa0400620 MtrunA17_Chr1g0187581 78.571 140 29 1 1 140 194 332 3.45e-78 241
Msa0400620 MtrunA17_Chr1g0183651 75.000 140 33 2 1 140 197 334 1.08e-71 224
Msa0400620 MtrunA17_Chr7g0261491 75.714 140 32 2 1 140 197 334 3.53e-71 223
Msa0400620 MtrunA17_Chr2g0299781 61.688 154 46 4 1 144 190 340 1.15e-55 182
Msa0400620 MtrunA17_Chr3g0135951 58.389 149 51 3 1 140 171 317 7.52e-54 177
Msa0400620 MtrunA17_Chr1g0153321 58.741 143 53 3 1 140 185 324 7.21e-50 166
Msa0400620 MtrunA17_Chr6g0478601 43.056 144 67 4 1 142 188 318 1.73e-27 106
Msa0400620 MtrunA17_Chr4g0018201 44.444 144 60 5 1 140 836 963 9.13e-27 104
Msa0400620 MtrunA17_Chr5g0428981 43.750 144 61 5 1 140 840 967 1.40e-26 103
Msa0400620 MtrunA17_Chr1g0197721 35.294 136 56 2 1 136 128 231 1.08e-19 84.3
Msa0400620 MtrunA17_Chr4g0030361 35.374 147 73 3 2 144 387 515 1.59e-18 80.9
Msa0400620 MtrunA17_Chr2g0304111 32.857 140 54 2 1 140 100 199 4.99e-18 79.3
Msa0400620 MtrunA17_Chr4g0074111 32.857 140 53 4 1 140 95 193 3.40e-17 76.6
Msa0400620 MtrunA17_Chr8g0369091 34.307 137 73 2 2 136 677 798 5.32e-17 76.6
Msa0400620 MtrunA17_Chr2g0309421 30.899 178 89 6 1 144 254 431 6.51e-17 76.3
Msa0400620 MtrunA17_Chr4g0059751 29.167 144 59 2 1 144 173 273 6.52e-17 76.3
Msa0400620 MtrunA17_Chr4g0013131 32.857 140 53 3 1 140 89 187 1.55e-16 75.1
Msa0400620 MtrunA17_Chr2g0327561 34.211 152 79 5 2 144 706 845 4.93e-16 73.9
Msa0400620 MtrunA17_Chr1g0188031 30.496 141 57 3 1 141 99 198 1.43e-15 72.4
Msa0400620 MtrunA17_Chr2g0312641 30.405 148 90 3 1 140 173 315 1.70e-15 72.0
Msa0400620 MtrunA17_Chr2g0284011 32.143 140 54 4 1 140 89 187 2.48e-15 71.6
Msa0400620 MtrunA17_Chr3g0080101 30.682 176 84 6 1 140 494 667 3.67e-15 71.2
Msa0400620 MtrunA17_Chr5g0432491 30.714 140 57 3 1 140 100 199 3.77e-15 71.2
Msa0400620 MtrunA17_Chr7g0266031 30.857 175 89 7 1 143 571 745 4.72e-15 70.9
Msa0400620 MtrunA17_Chr1g0179101 28.571 175 93 4 1 143 549 723 5.49e-15 70.9
Msa0400620 MtrunA17_Chr7g0227331 30.108 186 86 7 1 144 311 494 6.50e-15 70.5
Msa0400620 MtrunA17_Chr8g0335351 31.111 180 86 6 1 144 449 626 1.20e-14 69.7
Msa0400620 MtrunA17_Chr7g0245031 30.137 146 57 3 1 144 225 327 1.23e-14 69.7
Msa0400620 MtrunA17_Chr4g0063881 31.293 147 56 4 1 144 88 192 1.28e-14 69.7
Msa0400620 MtrunA17_Chr8g0346071 31.429 140 54 2 1 140 94 191 1.72e-14 69.3
Msa0400620 MtrunA17_Chr7g0244521 32.143 140 54 3 1 140 89 187 1.85e-14 69.3
Msa0400620 MtrunA17_Chr8g0346081 31.429 140 54 2 1 140 94 191 2.03e-14 68.9
Msa0400620 MtrunA17_Chr7g0250631 45.714 70 36 1 1 68 443 512 2.88e-14 68.9
Msa0400620 MtrunA17_Chr1g0205421 27.857 140 60 2 1 140 89 187 2.90e-14 68.6
Msa0400620 MtrunA17_Chr3g0107051 30.000 140 57 3 1 140 98 196 2.90e-14 68.6
Msa0400620 MtrunA17_Chr1g0150321 32.143 140 54 4 1 140 90 188 4.65e-14 68.2
Msa0400620 MtrunA17_Chr5g0402661 32.886 149 52 4 1 144 91 196 5.14e-14 67.8
Msa0400620 MtrunA17_Chr4g0032761 28.402 169 87 5 1 136 99 266 7.32e-14 67.4
Msa0400620 MtrunA17_Chr5g0441011 30.714 140 57 3 1 140 90 189 8.23e-14 67.4
Msa0400620 MtrunA17_Chr4g0063311 28.729 181 92 4 1 144 175 355 8.26e-14 67.4
Msa0400620 MtrunA17_Chr8g0379091 32.143 140 54 5 1 140 103 201 1.03e-13 67.0
Msa0400620 MtrunA17_Chr7g0272841 31.429 140 56 2 1 140 150 249 1.12e-13 67.0
Msa0400620 MtrunA17_Chr6g0453681 41.176 68 40 0 1 68 96 163 1.29e-13 66.6
Msa0400620 MtrunA17_Chr6g0468751 41.176 68 40 0 1 68 96 163 1.37e-13 66.6
Msa0400620 MtrunA17_Chr4g0001331 31.973 147 56 5 1 144 97 202 1.74e-13 66.2
Msa0400620 MtrunA17_Chr2g0284031 31.429 140 55 4 1 140 108 206 1.76e-13 66.6
Msa0400620 MtrunA17_Chr3g0107011 30.000 140 57 3 1 140 91 189 1.89e-13 66.2
Msa0400620 MtrunA17_Chr2g0290691 42.857 70 38 1 1 68 283 352 2.35e-13 66.2
Msa0400620 MtrunA17_Chr5g0432471 30.000 140 57 3 1 140 89 187 2.49e-13 65.9
Msa0400620 MtrunA17_Chr5g0428031 28.571 140 58 2 1 140 86 183 3.70e-13 65.5
Msa0400620 MtrunA17_Chr8g0338741 28.313 166 87 6 1 140 107 266 5.92e-13 64.7
Msa0400620 MtrunA17_Chr3g0132671 29.412 136 53 2 1 136 126 218 8.78e-13 64.3
Msa0400620 MtrunA17_Chr2g0329011 28.000 150 86 4 3 140 95 234 1.01e-12 64.3
Msa0400620 MtrunA17_Chr8g0380661 30.612 147 55 4 1 144 77 179 1.41e-12 63.5
Msa0400620 MtrunA17_Chr8g0356291 29.444 180 89 6 1 144 206 383 1.99e-12 63.5
Msa0400620 MtrunA17_Chr2g0333821 30.612 147 58 5 1 144 91 196 2.84e-12 62.8
Msa0400620 MtrunA17_Chr2g0286501 28.676 136 60 1 1 136 120 218 6.75e-12 62.0
Msa0400620 MtrunA17_Chr7g0234641 26.087 161 98 3 1 140 97 257 8.67e-12 61.6
Msa0400620 MtrunA17_Chr1g0166101 30.612 147 58 4 1 144 102 207 1.04e-11 61.2
Msa0400620 MtrunA17_Chr2g0321101 37.681 69 41 1 2 68 172 240 1.13e-11 61.2
Msa0400620 MtrunA17_Chr3g0114511 29.167 144 59 5 1 144 109 209 1.42e-11 60.8
Msa0400620 MtrunA17_Chr2g0326531 36.842 76 48 0 1 76 204 279 1.58e-11 60.8
Msa0400620 MtrunA17_Chr3g0130151 36.842 76 48 0 1 76 323 398 2.72e-11 60.1
Msa0400620 MtrunA17_Chr4g0072831 41.176 68 40 0 1 68 66 133 3.06e-11 59.7
Msa0400620 MtrunA17_Chr2g0285711 40.000 70 40 1 1 68 166 235 3.71e-11 59.7
Msa0400620 MtrunA17_Chr3g0125381 29.054 148 61 4 1 146 29 134 4.03e-11 58.5
Msa0400620 MtrunA17_Chr6g0453671 35.294 68 44 0 1 68 96 163 7.20e-11 58.9
Msa0400620 MtrunA17_Chr1g0188991 39.437 71 41 1 1 69 90 160 8.70e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0400620 AT4G14350.2 77.143 140 31 1 1 140 196 334 3.86e-74 231
Msa0400620 AT4G14350.1 77.143 140 31 1 1 140 196 334 3.86e-74 231
Msa0400620 AT4G14350.4 77.143 140 31 1 1 140 196 334 3.86e-74 231
Msa0400620 AT4G14350.3 77.143 140 31 1 1 140 196 334 3.89e-74 231
Msa0400620 AT3G23310.1 76.056 142 31 2 1 140 197 337 6.90e-72 225
Msa0400620 AT2G20470.3 72.143 140 36 2 1 140 201 337 4.48e-69 217
Msa0400620 AT2G20470.2 72.143 140 36 2 1 140 201 337 1.54e-68 217
Msa0400620 AT2G20470.1 72.143 140 36 2 1 140 201 337 1.54e-68 217
Msa0400620 AT1G03920.2 72.143 140 37 2 1 140 101 238 1.61e-65 206
Msa0400620 AT1G03920.1 72.143 140 37 2 1 140 214 351 1.13e-64 206
Msa0400620 AT1G03920.3 72.143 140 37 2 1 140 214 351 1.13e-64 206
Msa0400620 AT4G33080.2 62.162 148 47 3 1 140 171 317 3.03e-56 183
Msa0400620 AT4G33080.1 62.162 148 47 3 1 140 171 317 3.41e-56 183
Msa0400620 AT2G19400.1 59.868 152 52 3 1 144 182 332 1.10e-54 179
Msa0400620 AT1G30640.3 61.111 144 51 3 1 140 104 246 1.72e-54 178
Msa0400620 AT1G30640.2 61.111 144 51 3 1 140 197 339 3.64e-54 179
Msa0400620 AT1G30640.1 61.111 144 51 3 1 140 197 339 3.64e-54 179
Msa0400620 AT5G09890.2 60.274 146 48 4 1 140 179 320 3.70e-51 170
Msa0400620 AT5G09890.1 60.274 146 48 4 1 140 179 320 4.09e-51 170
Msa0400620 AT5G62310.1 42.143 140 70 2 1 140 831 959 2.50e-27 106
Msa0400620 AT1G45160.1 42.857 147 65 5 1 144 747 877 5.48e-27 105
Msa0400620 AT3G17850.1 44.138 145 66 5 1 143 959 1090 5.53e-27 105
Msa0400620 AT1G45160.2 42.857 147 65 5 1 144 747 877 6.05e-27 105
Msa0400620 AT1G48490.4 41.958 143 66 3 1 143 906 1031 4.17e-26 103
Msa0400620 AT1G48490.3 41.958 143 66 3 1 143 905 1030 4.37e-26 103
Msa0400620 AT1G48490.2 41.958 143 66 3 1 143 905 1030 4.37e-26 103
Msa0400620 AT1G48490.1 41.958 143 66 3 1 143 905 1030 4.37e-26 103
Msa0400620 AT5G04510.3 35.294 136 56 2 1 136 121 224 1.09e-20 85.9
Msa0400620 AT5G04510.2 35.294 136 56 2 1 136 121 224 2.99e-20 85.9
Msa0400620 AT5G04510.1 35.294 136 56 2 1 136 121 224 3.88e-20 85.9
Msa0400620 AT3G10540.1 35.294 136 56 2 1 136 122 225 1.67e-19 84.0
Msa0400620 AT5G58140.3 34.043 141 76 3 2 140 655 780 3.28e-17 77.8
Msa0400620 AT5G55910.2 32.973 185 81 7 1 144 186 368 3.29e-17 77.4
Msa0400620 AT5G55910.1 32.973 185 81 7 1 144 186 368 3.29e-17 77.4
Msa0400620 AT5G58140.6 34.043 141 76 3 2 140 655 780 3.45e-17 77.4
Msa0400620 AT5G58140.7 34.043 141 76 3 2 140 655 780 3.45e-17 77.4
Msa0400620 AT5G58140.5 34.043 141 76 3 2 140 655 780 3.45e-17 77.4
Msa0400620 AT5G58140.2 34.043 141 76 3 2 140 655 780 3.45e-17 77.4
Msa0400620 AT5G58140.1 34.043 141 76 3 2 140 655 780 3.45e-17 77.4
Msa0400620 AT1G30270.3 32.857 140 54 3 1 140 11 110 9.61e-17 75.9
Msa0400620 AT4G14580.1 33.571 140 53 2 1 140 99 198 1.46e-16 75.5
Msa0400620 AT1G30270.2 31.429 140 56 2 1 140 108 207 2.12e-16 75.1
Msa0400620 AT1G30270.1 31.429 140 56 2 1 140 108 207 2.15e-16 75.1
Msa0400620 AT5G25110.1 32.143 140 54 3 1 140 120 218 3.00e-16 74.7
Msa0400620 AT3G52890.2 30.645 186 84 7 1 143 615 798 5.22e-16 74.3
Msa0400620 AT3G52890.4 30.645 186 84 7 1 143 615 798 5.22e-16 74.3
Msa0400620 AT3G52890.1 30.645 186 84 7 1 143 615 798 5.22e-16 74.3
Msa0400620 AT3G52890.3 30.645 186 84 7 1 143 615 798 5.22e-16 74.3
Msa0400620 AT5G45820.1 33.571 140 52 3 1 140 89 187 8.45e-16 73.6
Msa0400620 AT5G10930.1 32.143 140 54 3 1 140 90 188 9.57e-16 73.2
Msa0400620 AT2G26980.3 32.143 140 55 3 1 140 91 190 1.18e-15 73.2
Msa0400620 AT3G27580.2 30.769 182 86 6 1 144 259 438 1.24e-15 73.2
Msa0400620 AT3G27580.1 30.769 182 86 6 1 144 259 438 1.24e-15 73.2
Msa0400620 AT2G26980.1 32.143 140 55 3 1 140 91 190 1.27e-15 72.8
Msa0400620 AT2G26980.2 32.143 140 55 3 1 140 91 190 1.34e-15 71.6
Msa0400620 AT2G26980.4 32.143 140 55 3 1 140 101 200 1.35e-15 72.8
Msa0400620 AT1G16440.1 31.361 169 89 6 1 144 192 358 1.38e-15 72.8
Msa0400620 AT4G30960.1 31.429 140 55 3 1 140 101 199 1.39e-15 72.8
Msa0400620 AT2G25090.1 31.429 140 63 2 1 140 93 199 1.39e-15 72.8
Msa0400620 AT2G26980.5 32.143 140 55 3 1 140 91 190 1.44e-15 72.8
Msa0400620 AT4G26610.1 48.571 70 34 1 1 68 200 269 2.02e-15 72.4
Msa0400620 AT5G35410.2 33.333 147 53 4 1 144 88 192 2.73e-15 71.6
Msa0400620 AT5G35410.1 31.973 147 55 3 1 144 88 192 4.00e-15 71.6
Msa0400620 AT1G01140.3 30.612 147 59 3 1 144 96 202 6.59e-15 70.9
Msa0400620 AT5G47750.1 45.714 70 36 1 1 68 268 337 6.98e-15 70.9
Msa0400620 AT1G01140.2 30.612 147 59 3 1 144 96 202 7.10e-15 70.9
Msa0400620 AT1G01140.1 30.612 147 59 3 1 144 96 202 7.37e-15 70.9
Msa0400620 AT3G45780.2 44.156 77 36 2 2 71 741 817 9.07e-15 70.5
Msa0400620 AT3G45780.1 44.156 77 36 2 2 71 741 817 9.07e-15 70.5
Msa0400620 AT3G20830.1 32.919 161 89 5 1 144 104 262 9.25e-15 70.5
Msa0400620 AT4G13000.1 31.818 154 85 5 1 140 98 245 1.23e-14 70.1
Msa0400620 AT4G24400.2 31.429 140 54 3 1 140 86 183 1.45e-14 69.7
Msa0400620 AT5G01810.1 32.143 140 54 3 1 140 89 187 1.51e-14 69.7
Msa0400620 AT5G01810.2 32.143 140 54 3 1 140 89 187 1.51e-14 69.7
Msa0400620 AT4G24400.1 31.429 140 54 3 1 140 86 183 1.73e-14 69.7
Msa0400620 AT2G36350.1 29.032 186 85 7 1 143 636 817 1.83e-14 69.7
Msa0400620 AT5G01810.3 32.143 140 54 3 1 140 89 187 2.20e-14 69.3
Msa0400620 AT3G23000.1 32.857 140 53 3 1 140 103 201 2.27e-14 69.3
Msa0400620 AT2G30360.1 27.857 140 61 2 1 140 99 198 2.47e-14 69.3
Msa0400620 AT5G58380.1 31.429 140 55 2 1 140 89 187 2.63e-14 69.3
Msa0400620 AT1G79250.1 30.769 182 86 6 1 144 223 402 3.00e-14 68.9
Msa0400620 AT1G79250.3 30.769 182 86 6 1 144 223 402 3.00e-14 68.9
Msa0400620 AT1G79250.2 30.769 182 86 6 1 144 223 402 3.00e-14 68.9
Msa0400620 AT1G79250.4 30.769 182 86 6 1 144 223 402 3.00e-14 68.9
Msa0400620 AT1G51170.1 32.075 159 91 5 1 144 105 261 3.29e-14 68.9
Msa0400620 AT5G45810.1 31.429 140 55 4 1 140 105 203 4.63e-14 68.6
Msa0400620 AT1G54960.1 30.000 140 58 2 1 140 150 249 6.93e-14 68.2
Msa0400620 AT1G54960.2 30.000 140 58 2 1 140 150 249 6.93e-14 68.2
Msa0400620 AT2G44830.2 45.714 70 36 1 1 68 440 509 7.09e-14 68.2
Msa0400620 AT2G44830.3 45.714 70 36 1 1 68 440 509 7.09e-14 68.2
Msa0400620 AT2G44830.1 45.714 70 36 1 1 68 440 509 7.09e-14 68.2
Msa0400620 AT3G08730.1 28.767 146 59 3 1 144 211 313 8.58e-14 67.8
Msa0400620 AT3G08730.2 28.767 146 59 3 1 144 211 313 8.58e-14 67.8
Msa0400620 AT3G08720.1 28.767 146 59 3 1 144 217 319 1.11e-13 67.4
Msa0400620 AT3G08720.3 28.767 146 59 3 1 144 217 319 1.11e-13 67.4
Msa0400620 AT3G08720.4 28.767 146 59 3 1 144 217 319 1.11e-13 67.4
Msa0400620 AT3G08720.2 28.767 146 59 3 1 144 217 319 1.11e-13 67.4
Msa0400620 AT2G26700.1 42.857 70 38 1 1 68 166 235 1.23e-13 67.4
Msa0400620 AT4G18700.1 31.469 143 57 4 1 143 103 204 1.26e-13 67.4
Msa0400620 AT3G12690.1 29.070 172 92 6 1 144 262 431 1.82e-13 67.0
Msa0400620 AT3G12690.3 29.070 172 92 6 1 144 262 431 1.82e-13 67.0
Msa0400620 AT3G12690.2 29.070 172 92 6 1 144 262 431 1.82e-13 67.0
Msa0400620 AT5G18700.1 43.836 73 41 0 1 73 75 147 1.95e-13 67.0
Msa0400620 AT3G44610.1 27.374 179 94 5 2 144 157 335 2.05e-13 66.6
Msa0400620 AT1G09000.2 30.714 140 57 2 1 140 151 250 2.95e-13 66.2
Msa0400620 AT1G09000.1 30.714 140 57 2 1 140 151 250 2.96e-13 66.2
Msa0400620 AT1G29230.1 29.371 143 60 3 1 143 151 252 3.18e-13 66.2
Msa0400620 AT3G01090.3 41.176 68 40 0 1 68 96 163 3.20e-13 66.2
Msa0400620 AT3G01090.1 41.176 68 40 0 1 68 96 163 3.20e-13 66.2
Msa0400620 AT3G29160.3 39.706 68 41 0 1 68 97 164 3.28e-13 65.9
Msa0400620 AT3G29160.1 39.706 68 41 0 1 68 97 164 3.30e-13 66.2
Msa0400620 AT3G29160.2 39.706 68 41 0 1 68 97 164 3.30e-13 66.2
Msa0400620 AT3G01090.2 41.176 68 40 0 1 68 119 186 3.36e-13 65.9
Msa0400620 AT5G21326.1 29.286 140 59 2 1 140 90 189 1.84e-12 63.9
Msa0400620 AT5G03640.2 27.222 180 94 5 1 143 618 797 2.23e-12 63.9
Msa0400620 AT5G03640.1 27.222 180 94 5 1 143 618 797 2.23e-12 63.9
Msa0400620 AT2G34650.1 31.373 153 80 4 1 135 157 302 2.36e-12 63.5
Msa0400620 AT5G39440.1 38.235 68 42 0 1 68 96 163 7.03e-12 62.4
Msa0400620 AT3G14370.1 27.333 150 102 2 1 143 165 314 7.69e-12 62.0
Msa0400620 AT3G06030.1 29.286 140 59 2 1 140 150 249 9.22e-12 62.0
Msa0400620 AT2G45490.2 44.118 68 38 0 1 68 99 166 1.07e-11 60.8
Msa0400620 AT2G45490.1 44.118 68 38 0 1 68 99 166 1.34e-11 60.8
Msa0400620 AT1G53700.1 36.000 75 46 1 1 73 171 245 1.36e-11 61.2
Msa0400620 AT3G25250.1 29.487 156 90 4 1 140 101 252 1.82e-11 60.8
Msa0400620 AT5G21222.1 28.571 140 60 3 1 140 90 189 2.77e-11 60.5
Msa0400620 AT5G21222.2 28.571 140 60 3 1 140 90 189 2.77e-11 60.5
Msa0400620 AT5G21222.4 28.571 140 60 3 1 140 90 189 2.77e-11 60.5
Msa0400620 AT5G21222.3 28.571 140 60 3 1 140 90 189 2.77e-11 60.5
Msa0400620 AT5G40030.1 38.571 70 41 1 1 68 191 260 3.33e-11 60.5
Msa0400620 AT5G57630.3 30.000 140 54 4 1 140 1 96 3.56e-11 60.1
Msa0400620 AT5G57630.1 30.000 140 54 4 1 140 89 184 5.61e-11 59.7
Msa0400620 AT5G57565.2 39.706 68 40 1 1 68 36 102 5.85e-11 57.0
Msa0400620 AT5G57565.6 39.706 68 40 1 1 68 36 102 5.85e-11 57.0
Msa0400620 AT2G37840.1 34.146 82 51 1 1 79 88 169 6.02e-11 59.7
Msa0400620 AT5G57630.2 30.000 140 54 4 1 140 89 184 7.29e-11 59.3
Msa0400620 AT2G34180.1 28.571 140 59 3 1 140 134 232 7.85e-11 59.3
Msa0400620 AT5G57565.5 39.706 68 40 1 1 68 36 102 8.29e-11 57.0
Msa0400620 AT5G57565.1 39.706 68 40 1 1 68 36 102 8.29e-11 57.0

Find 34 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACGTGAAATTATCAGATTT+TGG 0.253070 3_2:+25073511 Msa0400620:CDS
CCAGTTGCTGCTGTTGTGAT+TGG 0.310492 3_2:-25073639 None:intergenic
ATGTTTATGAATAGACTCTA+TGG 0.331837 3_2:-25073201 None:intergenic
CAAGCCCGACAACTTGCTTC+TGG 0.332235 3_2:+25073477 Msa0400620:CDS
AGTATTTATATCTCATATAA+TGG 0.365878 3_2:+25073082 Msa0400620:exon
AACTTCTGGAGCAATATAAT+CGG 0.385695 3_2:-25074113 None:intergenic
GCTAGACTGCAGTAACCTTC+AGG 0.390289 3_2:+25073546 Msa0400620:CDS
ATAAACATAATTATATTCAT+AGG 0.410728 3_2:+25073216 Msa0400620:CDS
CCTCTCTTCAGAATAACTTC+TGG 0.412241 3_2:-25074127 None:intergenic
AAGCAAGTTCATCACTTTGA+AGG 0.415965 3_2:-25073606 None:intergenic
CCAATCACAACAGCAGCAAC+TGG 0.428613 3_2:+25073639 Msa0400620:CDS
AGCAGCAACTGGAGCATTTG+CGG 0.457627 3_2:+25073650 Msa0400620:CDS
GATATTGCAGGCCTATTCTA+CGG 0.462226 3_2:+25074081 Msa0400620:intron
CTATCCAGAAGCAAGTTGTC+GGG 0.469273 3_2:-25073481 None:intergenic
CTTTATTGTTCCTTTCAAGA+TGG 0.472271 3_2:+25073057 Msa0400620:five_prime_UTR
TGCTATCAACTTCTGCAAGA+AGG 0.478344 3_2:-25073020 None:intergenic
GGGTGTTCCAACCGTAGAAT+AGG 0.479245 3_2:-25074092 None:intergenic
TCTAATGGAGTTAACATAAG+TGG 0.486303 3_2:+25073580 Msa0400620:CDS
TGCTGCTGTTGTGATTGGTT+AGG 0.492962 3_2:-25073634 None:intergenic
TAAATACTCGCCATCTTGAA+AGG 0.513200 3_2:-25073067 None:intergenic
TCTATCCAGAAGCAAGTTGT+CGG 0.513902 3_2:-25073482 None:intergenic
TTGCAGGCCTATTCTACGGT+TGG 0.530006 3_2:+25074085 Msa0400620:intron
CAACGTCATCATATCTCCAC+CGG 0.544529 3_2:-25073114 None:intergenic
ACTTCTGGAGCAATATAATC+GGG 0.545377 3_2:-25074112 None:intergenic
AACTTGCTTCTGGATAGAAA+TGG 0.545939 3_2:+25073487 Msa0400620:CDS
TTGAATATGTCAAATCTGAA+AGG 0.568344 3_2:+25072995 None:intergenic
CATATAATGGAGTATCTACC+CGG 0.572784 3_2:+25073095 Msa0400620:exon
CCAGAAGTTATTCTGAAGAG+AGG 0.595321 3_2:+25074127 Msa0400620:CDS
CAGAAGTTATTCTGAAGAGA+GGG 0.602815 3_2:+25074128 Msa0400620:CDS
TGAAGGTTACTGCAGTCTAG+CGG 0.630065 3_2:-25073544 None:intergenic
ATATGATGACGTTGTTGATG+CGG 0.636502 3_2:+25073123 Msa0400620:CDS
TGAAGCTAAGTTCTATATCG+AGG 0.653871 3_2:+25073166 Msa0400620:CDS
AACGTCATCATATCTCCACC+GGG 0.670602 3_2:-25073113 None:intergenic
ATAATGGAGTATCTACCCGG+TGG 0.735358 3_2:+25073098 Msa0400620:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAACATAATTATATTCAT+AGG + chr3_2:25073216-25073235 Msa0400620:CDS 10.0%
!!! TTTTTATTATAACTGAAAAA+AGG - chr3_2:25073848-25073867 None:intergenic 10.0%
!! ATAAACATAATTATATTCAT+AGG + chr3_2:25073216-25073235 Msa0400620:CDS 10.0%
!!! TTTTTATTATAACTGAAAAA+AGG - chr3_2:25073848-25073867 None:intergenic 10.0%
!! AGTATTTATATCTCATATAA+TGG + chr3_2:25073082-25073101 Msa0400620:exon 15.0%
!! TAGCAAACTTTATTATAAAA+CGG + chr3_2:25073748-25073767 Msa0400620:intron 15.0%
!!! TCTTTTGTTATATTTTTGTT+TGG + chr3_2:25073905-25073924 Msa0400620:intron 15.0%
!! ATTTACATCATATATCAATT+TGG + chr3_2:25073951-25073970 Msa0400620:intron 15.0%
!! TTTACATCATATATCAATTT+GGG + chr3_2:25073952-25073971 Msa0400620:intron 15.0%
!! AGTATTTATATCTCATATAA+TGG + chr3_2:25073082-25073101 Msa0400620:exon 15.0%
!! TAGCAAACTTTATTATAAAA+CGG + chr3_2:25073748-25073767 Msa0400620:intron 15.0%
!!! TCTTTTGTTATATTTTTGTT+TGG + chr3_2:25073905-25073924 Msa0400620:intron 15.0%
!! ATTTACATCATATATCAATT+TGG + chr3_2:25073951-25073970 Msa0400620:intron 15.0%
!! TTTACATCATATATCAATTT+GGG + chr3_2:25073952-25073971 Msa0400620:intron 15.0%
!! AAAGAGAGAATAATCTTTAA+AGG - chr3_2:25073997-25074016 None:intergenic 20.0%
!! AAAGAGAGAATAATCTTTAA+AGG - chr3_2:25073997-25074016 None:intergenic 20.0%
! ATGTTTATGAATAGACTCTA+TGG - chr3_2:25073204-25073223 None:intergenic 25.0%
! AATAGTGATTACAGATTAAG+CGG - chr3_2:25073243-25073262 None:intergenic 25.0%
! AGTGATTTCGTTGAAAATAA+CGG - chr3_2:25073320-25073339 None:intergenic 25.0%
!! AATTTTCAGTGTTAGATTGT+AGG + chr3_2:25073350-25073369 Msa0400620:intron 25.0%
!! ATTTTCAGTGTTAGATTGTA+GGG + chr3_2:25073351-25073370 Msa0400620:intron 25.0%
! TATGATTACTTTGTTCAAAG+CGG + chr3_2:25073816-25073835 Msa0400620:intron 25.0%
!! TTATAACTGAAAAAAGGTGT+AGG - chr3_2:25073842-25073861 None:intergenic 25.0%
! CATTATTCTTTCGTTATGTT+TGG + chr3_2:25073874-25073893 Msa0400620:intron 25.0%
!!! TATTCTCTCTTTTGATTGTA+TGG + chr3_2:25074005-25074024 Msa0400620:intron 25.0%
!!! TCTATCTTTTTTGATATTGC+AGG + chr3_2:25074069-25074088 Msa0400620:intron 25.0%
! GTGATTGGTATAATAGTATA+TGG + chr3_2:25074164-25074183 Msa0400620:intron 25.0%
! ATGTTTATGAATAGACTCTA+TGG - chr3_2:25073204-25073223 None:intergenic 25.0%
! AATAGTGATTACAGATTAAG+CGG - chr3_2:25073243-25073262 None:intergenic 25.0%
! AGTGATTTCGTTGAAAATAA+CGG - chr3_2:25073320-25073339 None:intergenic 25.0%
!! AATTTTCAGTGTTAGATTGT+AGG + chr3_2:25073350-25073369 Msa0400620:intron 25.0%
!! ATTTTCAGTGTTAGATTGTA+GGG + chr3_2:25073351-25073370 Msa0400620:intron 25.0%
! TATGATTACTTTGTTCAAAG+CGG + chr3_2:25073816-25073835 Msa0400620:intron 25.0%
!! TTATAACTGAAAAAAGGTGT+AGG - chr3_2:25073842-25073861 None:intergenic 25.0%
! CATTATTCTTTCGTTATGTT+TGG + chr3_2:25073874-25073893 Msa0400620:intron 25.0%
!!! TATTCTCTCTTTTGATTGTA+TGG + chr3_2:25074005-25074024 Msa0400620:intron 25.0%
!!! TCTATCTTTTTTGATATTGC+AGG + chr3_2:25074069-25074088 Msa0400620:intron 25.0%
! GTGATTGGTATAATAGTATA+TGG + chr3_2:25074164-25074183 Msa0400620:intron 25.0%
CTTTATTGTTCCTTTCAAGA+TGG + chr3_2:25073057-25073076 Msa0400620:five_prime_UTR 30.0%
! CAACGAAATCACTATTGTTT+TGG + chr3_2:25073327-25073346 Msa0400620:intron 30.0%
! CACGTGAAATTATCAGATTT+TGG + chr3_2:25073511-25073530 Msa0400620:CDS 30.0%
! TAGATAAGTCTTTTTCCTGA+AGG - chr3_2:25073564-25073583 None:intergenic 30.0%
CAGGAAAAAGACTTATCTAA+TGG + chr3_2:25073565-25073584 Msa0400620:CDS 30.0%
TCTAATGGAGTTAACATAAG+TGG + chr3_2:25073580-25073599 Msa0400620:CDS 30.0%
!! AACTTCTGGAGCAATATAAT+CGG - chr3_2:25074116-25074135 None:intergenic 30.0%
GAATAAGTGAATAACTTCTC+AGG + chr3_2:25074218-25074237 Msa0400620:intron 30.0%
CTTTATTGTTCCTTTCAAGA+TGG + chr3_2:25073057-25073076 Msa0400620:five_prime_UTR 30.0%
! CAACGAAATCACTATTGTTT+TGG + chr3_2:25073327-25073346 Msa0400620:intron 30.0%
! CACGTGAAATTATCAGATTT+TGG + chr3_2:25073511-25073530 Msa0400620:CDS 30.0%
! TAGATAAGTCTTTTTCCTGA+AGG - chr3_2:25073564-25073583 None:intergenic 30.0%
CAGGAAAAAGACTTATCTAA+TGG + chr3_2:25073565-25073584 Msa0400620:CDS 30.0%
TCTAATGGAGTTAACATAAG+TGG + chr3_2:25073580-25073599 Msa0400620:CDS 30.0%
!! AACTTCTGGAGCAATATAAT+CGG - chr3_2:25074116-25074135 None:intergenic 30.0%
GAATAAGTGAATAACTTCTC+AGG + chr3_2:25074218-25074237 Msa0400620:intron 30.0%
TAAATACTCGCCATCTTGAA+AGG - chr3_2:25073070-25073089 None:intergenic 35.0%
! CATATAATGGAGTATCTACC+CGG + chr3_2:25073095-25073114 Msa0400620:exon 35.0%
! ATATGATGACGTTGTTGATG+CGG + chr3_2:25073123-25073142 Msa0400620:CDS 35.0%
TGAAGCTAAGTTCTATATCG+AGG + chr3_2:25073166-25073185 Msa0400620:CDS 35.0%
GATCTTGAATACCGATCATT+CGG + chr3_2:25073393-25073412 Msa0400620:intron 35.0%
AATGAATACCTCCGAATGAT+CGG - chr3_2:25073407-25073426 None:intergenic 35.0%
! AACTTGCTTCTGGATAGAAA+TGG + chr3_2:25073487-25073506 Msa0400620:CDS 35.0%
AAGCAAGTTCATCACTTTGA+AGG - chr3_2:25073609-25073628 None:intergenic 35.0%
CTTTCGTTATGTTTGGACTT+CGG + chr3_2:25073881-25073900 Msa0400620:intron 35.0%
!! ACTTCTGGAGCAATATAATC+GGG - chr3_2:25074115-25074134 None:intergenic 35.0%
! CAGAAGTTATTCTGAAGAGA+GGG + chr3_2:25074128-25074147 Msa0400620:CDS 35.0%
TAAATACTCGCCATCTTGAA+AGG - chr3_2:25073070-25073089 None:intergenic 35.0%
! CATATAATGGAGTATCTACC+CGG + chr3_2:25073095-25073114 Msa0400620:exon 35.0%
! ATATGATGACGTTGTTGATG+CGG + chr3_2:25073123-25073142 Msa0400620:CDS 35.0%
TGAAGCTAAGTTCTATATCG+AGG + chr3_2:25073166-25073185 Msa0400620:CDS 35.0%
GATCTTGAATACCGATCATT+CGG + chr3_2:25073393-25073412 Msa0400620:intron 35.0%
AATGAATACCTCCGAATGAT+CGG - chr3_2:25073407-25073426 None:intergenic 35.0%
! AACTTGCTTCTGGATAGAAA+TGG + chr3_2:25073487-25073506 Msa0400620:CDS 35.0%
AAGCAAGTTCATCACTTTGA+AGG - chr3_2:25073609-25073628 None:intergenic 35.0%
CTTTCGTTATGTTTGGACTT+CGG + chr3_2:25073881-25073900 Msa0400620:intron 35.0%
!! ACTTCTGGAGCAATATAATC+GGG - chr3_2:25074115-25074134 None:intergenic 35.0%
! CAGAAGTTATTCTGAAGAGA+GGG + chr3_2:25074128-25074147 Msa0400620:CDS 35.0%
TGCTATCAACTTCTGCAAGA+AGG - chr3_2:25073023-25073042 None:intergenic 40.0%
GGGTACTTAACTTTACGACA+TGG + chr3_2:25073371-25073390 Msa0400620:intron 40.0%
TCTATCCAGAAGCAAGTTGT+CGG - chr3_2:25073485-25073504 None:intergenic 40.0%
GATATTGCAGGCCTATTCTA+CGG + chr3_2:25074081-25074100 Msa0400620:intron 40.0%
CCTCTCTTCAGAATAACTTC+TGG - chr3_2:25074130-25074149 None:intergenic 40.0%
! CCAGAAGTTATTCTGAAGAG+AGG + chr3_2:25074127-25074146 Msa0400620:CDS 40.0%
! GTTATTCTGAAGAGAGGGTA+TGG + chr3_2:25074133-25074152 Msa0400620:intron 40.0%
! AGTATATGGCGTTGAAAGCT+TGG + chr3_2:25074178-25074197 Msa0400620:intron 40.0%
TGCTATCAACTTCTGCAAGA+AGG - chr3_2:25073023-25073042 None:intergenic 40.0%
GGGTACTTAACTTTACGACA+TGG + chr3_2:25073371-25073390 Msa0400620:intron 40.0%
TCTATCCAGAAGCAAGTTGT+CGG - chr3_2:25073485-25073504 None:intergenic 40.0%
GATATTGCAGGCCTATTCTA+CGG + chr3_2:25074081-25074100 Msa0400620:intron 40.0%
CCTCTCTTCAGAATAACTTC+TGG - chr3_2:25074130-25074149 None:intergenic 40.0%
! CCAGAAGTTATTCTGAAGAG+AGG + chr3_2:25074127-25074146 Msa0400620:CDS 40.0%
! GTTATTCTGAAGAGAGGGTA+TGG + chr3_2:25074133-25074152 Msa0400620:intron 40.0%
! AGTATATGGCGTTGAAAGCT+TGG + chr3_2:25074178-25074197 Msa0400620:intron 40.0%
! ATAATGGAGTATCTACCCGG+TGG + chr3_2:25073098-25073117 Msa0400620:exon 45.0%
AACGTCATCATATCTCCACC+GGG - chr3_2:25073116-25073135 None:intergenic 45.0%
CAACGTCATCATATCTCCAC+CGG - chr3_2:25073117-25073136 None:intergenic 45.0%
CTTGAATACCGATCATTCGG+AGG + chr3_2:25073396-25073415 Msa0400620:intron 45.0%
CTATCCAGAAGCAAGTTGTC+GGG - chr3_2:25073484-25073503 None:intergenic 45.0%
TGAAGGTTACTGCAGTCTAG+CGG - chr3_2:25073547-25073566 None:intergenic 45.0%
! TGCTGCTGTTGTGATTGGTT+AGG - chr3_2:25073637-25073656 None:intergenic 45.0%
GAAAAATCGCAGCAGAATGC+TGG + chr3_2:25073672-25073691 Msa0400620:CDS 45.0%
! ATAATGGAGTATCTACCCGG+TGG + chr3_2:25073098-25073117 Msa0400620:exon 45.0%
AACGTCATCATATCTCCACC+GGG - chr3_2:25073116-25073135 None:intergenic 45.0%
CAACGTCATCATATCTCCAC+CGG - chr3_2:25073117-25073136 None:intergenic 45.0%
CTTGAATACCGATCATTCGG+AGG + chr3_2:25073396-25073415 Msa0400620:intron 45.0%
CTATCCAGAAGCAAGTTGTC+GGG - chr3_2:25073484-25073503 None:intergenic 45.0%
TGAAGGTTACTGCAGTCTAG+CGG - chr3_2:25073547-25073566 None:intergenic 45.0%
! TGCTGCTGTTGTGATTGGTT+AGG - chr3_2:25073637-25073656 None:intergenic 45.0%
GAAAAATCGCAGCAGAATGC+TGG + chr3_2:25073672-25073691 Msa0400620:CDS 45.0%
GCTAGACTGCAGTAACCTTC+AGG + chr3_2:25073546-25073565 Msa0400620:CDS 50.0%
CCAGTTGCTGCTGTTGTGAT+TGG - chr3_2:25073642-25073661 None:intergenic 50.0%
CCAATCACAACAGCAGCAAC+TGG + chr3_2:25073639-25073658 Msa0400620:CDS 50.0%
! AGCAGCAACTGGAGCATTTG+CGG + chr3_2:25073650-25073669 Msa0400620:CDS 50.0%
TTGCAGGCCTATTCTACGGT+TGG + chr3_2:25074085-25074104 Msa0400620:intron 50.0%
GGGTGTTCCAACCGTAGAAT+AGG - chr3_2:25074095-25074114 None:intergenic 50.0%
GGTATGGCGTGGAATGTGAT+TGG + chr3_2:25074149-25074168 Msa0400620:intron 50.0%
GCTAGACTGCAGTAACCTTC+AGG + chr3_2:25073546-25073565 Msa0400620:CDS 50.0%
CCAGTTGCTGCTGTTGTGAT+TGG - chr3_2:25073642-25073661 None:intergenic 50.0%
CCAATCACAACAGCAGCAAC+TGG + chr3_2:25073639-25073658 Msa0400620:CDS 50.0%
! AGCAGCAACTGGAGCATTTG+CGG + chr3_2:25073650-25073669 Msa0400620:CDS 50.0%
TTGCAGGCCTATTCTACGGT+TGG + chr3_2:25074085-25074104 Msa0400620:intron 50.0%
GGGTGTTCCAACCGTAGAAT+AGG - chr3_2:25074095-25074114 None:intergenic 50.0%
GGTATGGCGTGGAATGTGAT+TGG + chr3_2:25074149-25074168 Msa0400620:intron 50.0%
! CAAGCCCGACAACTTGCTTC+TGG + chr3_2:25073477-25073496 Msa0400620:CDS 55.0%
! TCTGAAGAGAGGGTATGGCG+TGG + chr3_2:25074138-25074157 Msa0400620:intron 55.0%
! CAAGCCCGACAACTTGCTTC+TGG + chr3_2:25073477-25073496 Msa0400620:CDS 55.0%
! TCTGAAGAGAGGGTATGGCG+TGG + chr3_2:25074138-25074157 Msa0400620:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 25073004 25074261 25073004 ID=Msa0400620;Name=Msa0400620
chr3_2 mRNA 25073004 25074261 25073004 ID=Msa0400620-mRNA-1;Parent=Msa0400620;Name=Msa0400620-mRNA-1;_AED=0.26;_eAED=0.26;_QI=97|0.33|0.25|0.5|1|1|4|0|146
chr3_2 exon 25073004 25073237 25073004 ID=Msa0400620-mRNA-1:exon:5217;Parent=Msa0400620-mRNA-1
chr3_2 exon 25073471 25073693 25073471 ID=Msa0400620-mRNA-1:exon:5218;Parent=Msa0400620-mRNA-1
chr3_2 exon 25074091 25074149 25074091 ID=Msa0400620-mRNA-1:exon:5219;Parent=Msa0400620-mRNA-1
chr3_2 exon 25074240 25074261 25074240 ID=Msa0400620-mRNA-1:exon:5220;Parent=Msa0400620-mRNA-1
chr3_2 five_prime_UTR 25073004 25073100 25073004 ID=Msa0400620-mRNA-1:five_prime_utr;Parent=Msa0400620-mRNA-1
chr3_2 CDS 25073101 25073237 25073101 ID=Msa0400620-mRNA-1:cds;Parent=Msa0400620-mRNA-1
chr3_2 CDS 25073471 25073693 25073471 ID=Msa0400620-mRNA-1:cds;Parent=Msa0400620-mRNA-1
chr3_2 CDS 25074091 25074149 25074091 ID=Msa0400620-mRNA-1:cds;Parent=Msa0400620-mRNA-1
chr3_2 CDS 25074240 25074261 25074240 ID=Msa0400620-mRNA-1:cds;Parent=Msa0400620-mRNA-1
Gene Sequence

>Msa0400620

ATGGAGTATCTACCCGGTGGAGATATGATGACGTTGTTGATGCGGAAAGAAGTACTGACTGAAGATGAAGCTAAGTTCTATATCGAGGAGACTGTGCTAGCCATAGAGTCTATTCATAAACATAATTATATTCATAGAGATATCAAGCCCGACAACTTGCTTCTGGATAGAAATGGTCACGTGAAATTATCAGATTTTGGATTATGTAAGCCGCTAGACTGCAGTAACCTTCAGGAAAAAGACTTATCTAATGGAGTTAACATAAGTGGAGTCCTTCAAAGTGATGAACTTGCTTTATCTCCTAACCAATCACAACAGCAGCAACTGGAGCATTTGCGGAAAAATCGCAGCAGAATGCTGGCCTATTCTACGGTTGGAACACCCGATTATATTGCTCCAGAAGTTATTCTGAAGAGAGGGTCTGCTGCTGATAATCTCTAA
Protein sequence

>Msa0400620

MEYLPGGDMMTLLMRKEVLTEDEAKFYIEETVLAIESIHKHNYIHRDIKPDNLLLDRNGHVKLSDFGLCKPLDCSNLQEKDLSNGVNISGVLQSDELALSPNQSQQQQLEHLRKNRSRMLAYSTVGTPDYIAPEVILKRGSAADNL*