Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0350010 | Msa0409960 | 0.800761 | 1.238355e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGACCTGTTGTGGATTCA+TGG | 0.312516 | 3_2:+51282465 | None:intergenic |
| AGAGATCTTTGCTTGATATC+AGG | 0.323362 | 3_2:+51284782 | None:intergenic |
| GTTATGGTTGTAGTTAAGAT+TGG | 0.340238 | 3_2:-51284833 | Msa0409960:CDS |
| CTCCGTTCTAGTTTCTTAAG+AGG | 0.374795 | 3_2:-51282333 | Msa0409960:three_prime_UTR |
| TATTGTAGGTTTGCTTTACT+AGG | 0.380164 | 3_2:-51282541 | Msa0409960:intron |
| TTATGGTTGTAGTTAAGATT+GGG | 0.434818 | 3_2:-51284832 | Msa0409960:CDS |
| AACTATTGATGACTTAAGCT+TGG | 0.453768 | 3_2:+51282285 | None:intergenic |
| GTTTGCTTTACTAGGATGAC+AGG | 0.456534 | 3_2:-51282533 | Msa0409960:CDS |
| CAATCTTTGAATCATATTTG+AGG | 0.477529 | 3_2:+51282368 | None:intergenic |
| AAGCTTAAGTCATCAATAGT+TGG | 0.526133 | 3_2:-51282283 | Msa0409960:three_prime_UTR |
| CTAACATCTTACAAACTACA+TGG | 0.534008 | 3_2:+51282225 | None:intergenic |
| GAGCACCATGAATCCACAAC+AGG | 0.538023 | 3_2:-51282470 | Msa0409960:CDS |
| AAATTTCAGAAGAATAAGAA+TGG | 0.538781 | 3_2:-51282494 | Msa0409960:CDS |
| CTTTGAATCATATTTGAGGC+CGG | 0.545057 | 3_2:+51282372 | None:intergenic |
| TGGTTGTAGTTAAGATTGGG+AGG | 0.582462 | 3_2:-51284829 | Msa0409960:CDS |
| TTAAGAAACTAGAACGGAGA+AGG | 0.582678 | 3_2:+51282337 | None:intergenic |
| TTTGCTTTACTAGGATGACA+GGG | 0.623686 | 3_2:-51282532 | Msa0409960:CDS |
| CTCCTCTTAAGAAACTAGAA+CGG | 0.632518 | 3_2:+51282331 | None:intergenic |
| ACAACCGCTGAAGTGTGTAC+CGG | 0.651271 | 3_2:-51282391 | Msa0409960:three_prime_UTR |
| ATCTTGTAGACGAGTGACAG+TGG | 0.658091 | 3_2:-51282439 | Msa0409960:exon |
| GAGGCCGGTACACACTTCAG+CGG | 0.680988 | 3_2:+51282387 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGTCAAAATATTTT+TGG | - | chr3_2:51282551-51282570 | Msa0409960:intron | 10.0% |
| !! | AAGTATATTATAATTCTTAA+TGG | + | chr3_2:51284411-51284430 | None:intergenic | 10.0% |
| !! | ATTATAATGAATTAGTGATT+AGG | - | chr3_2:51282411-51282430 | Msa0409960:three_prime_UTR | 15.0% |
| !! | TTATAATGAATTAGTGATTA+GGG | - | chr3_2:51282412-51282431 | Msa0409960:three_prime_UTR | 15.0% |
| !!! | TTTATCATTTTTTTTTCTTG+AGG | - | chr3_2:51283304-51283323 | Msa0409960:intron | 15.0% |
| !! | GAAATTGTAATTATTATTGT+AGG | - | chr3_2:51284468-51284487 | Msa0409960:intron | 15.0% |
| !!! | GGTATTAATTAAGTTTTATA+AGG | - | chr3_2:51284761-51284780 | Msa0409960:intron | 15.0% |
| !!! | ATCTTCTTTAAAGTTTGAAA+TGG | - | chr3_2:51282285-51282304 | Msa0409960:three_prime_UTR | 20.0% |
| !! | AATAATTTAACATATACCTC+AGG | + | chr3_2:51282353-51282372 | None:intergenic | 20.0% |
| !!! | TATATGTGAAATTGTTTTTG+TGG | - | chr3_2:51282372-51282391 | Msa0409960:three_prime_UTR | 20.0% |
| !!! | TTTGACTTAATTTTATCTTG+TGG | + | chr3_2:51282544-51282563 | None:intergenic | 20.0% |
| !! | TGCAATCAATTTGTAAAATT+AGG | - | chr3_2:51282830-51282849 | Msa0409960:intron | 20.0% |
| !! | AAATATAGAAAATAAGCATG+TGG | + | chr3_2:51283149-51283168 | None:intergenic | 20.0% |
| !! | ATTTCAGCATAAATATAGAA+GGG | + | chr3_2:51283370-51283389 | None:intergenic | 20.0% |
| !! | AATAAAATATGAAAGCAACA+AGG | + | chr3_2:51283627-51283646 | None:intergenic | 20.0% |
| !!! | GAGGTATTAAAGATATAAAT+TGG | - | chr3_2:51283868-51283887 | Msa0409960:intron | 20.0% |
| !!! | CAATTGGAAGTATTTTAATT+AGG | + | chr3_2:51284440-51284459 | None:intergenic | 20.0% |
| !! | ACAATTTCAAACATATCAAT+TGG | + | chr3_2:51284456-51284475 | None:intergenic | 20.0% |
| !! | AAATTTCAGAAGAATAAGAA+TGG | - | chr3_2:51284529-51284548 | Msa0409960:intron | 20.0% |
| ! | TTAGTGATTAGGGATTTAAT+TGG | - | chr3_2:51282422-51282441 | Msa0409960:three_prime_UTR | 25.0% |
| ! | ATTTAATTGGACTGAAACTT+AGG | - | chr3_2:51282435-51282454 | Msa0409960:exon | 25.0% |
| !!! | ATTAATGTAGACCTACTTTT+CGG | - | chr3_2:51282937-51282956 | Msa0409960:intron | 25.0% |
| ! | AATCATTCAAAACAAAAGTG+AGG | + | chr3_2:51283046-51283065 | None:intergenic | 25.0% |
| !!! | TAATTGATATTGGTTCTTAC+TGG | - | chr3_2:51283195-51283214 | Msa0409960:intron | 25.0% |
| ! | GATTTCAGCATAAATATAGA+AGG | + | chr3_2:51283371-51283390 | None:intergenic | 25.0% |
| ! | CACTATAAACTTTGGATTAT+AGG | - | chr3_2:51283458-51283477 | Msa0409960:intron | 25.0% |
| ! | AAACTTTGGATTATAGGATA+CGG | - | chr3_2:51283464-51283483 | Msa0409960:intron | 25.0% |
| !!! | AGACATGATAACTCTTTTTT+TGG | - | chr3_2:51283750-51283769 | Msa0409960:intron | 25.0% |
| !!! | GGTTCTTTTATTTACACAAT+TGG | - | chr3_2:51283771-51283790 | Msa0409960:intron | 25.0% |
| ! | ATTTACACAATTGGATTTCA+CGG | - | chr3_2:51283780-51283799 | Msa0409960:intron | 25.0% |
| !!! | ACTTTTAAAGAGTTTCAAAG+AGG | - | chr3_2:51283849-51283868 | Msa0409960:intron | 25.0% |
| ! | ATTAAAATATGACCTGATAG+CGG | - | chr3_2:51284178-51284197 | Msa0409960:intron | 25.0% |
| !!! | TTGTTGATGAGATTTCTTTT+AGG | - | chr3_2:51284242-51284261 | Msa0409960:intron | 25.0% |
| ! | CAATCTTTGAATCATATTTG+AGG | + | chr3_2:51284658-51284677 | None:intergenic | 25.0% |
| !! | GTTTTATAAGGATAAGAGTT+TGG | - | chr3_2:51284773-51284792 | Msa0409960:CDS | 25.0% |
| ATACAAATAAATGTGTCCTG+AGG | - | chr3_2:51282334-51282353 | Msa0409960:three_prime_UTR | 30.0% | |
| !!! | AGAGCTTATTTTGTAGCTTA+TGG | - | chr3_2:51282472-51282491 | Msa0409960:CDS | 30.0% |
| ! | ATTTATTTGTTGCAAGAGTG+AGG | - | chr3_2:51282676-51282695 | Msa0409960:intron | 30.0% |
| ! | ATAAGCTTTTCACTGAAACA+AGG | + | chr3_2:51282708-51282727 | None:intergenic | 30.0% |
| ! | TTTGTTGCTTATAGCAGAAA+AGG | - | chr3_2:51282728-51282747 | Msa0409960:intron | 30.0% |
| ! | TTGTTGCTTATAGCAGAAAA+GGG | - | chr3_2:51282729-51282748 | Msa0409960:intron | 30.0% |
| TTGCAGATATTCATTTCACT+TGG | + | chr3_2:51282815-51282834 | None:intergenic | 30.0% | |
| TGAAGCTTGCATTAACTTTA+TGG | - | chr3_2:51283105-51283124 | Msa0409960:intron | 30.0% | |
| TGTCACTCACTAATTGATAT+TGG | - | chr3_2:51283185-51283204 | Msa0409960:intron | 30.0% | |
| ACTGGATAAATGAACACTAA+AGG | - | chr3_2:51283213-51283232 | Msa0409960:intron | 30.0% | |
| TGCTTGACTTAGATAATGAT+TGG | - | chr3_2:51283251-51283270 | Msa0409960:intron | 30.0% | |
| ACTACACCATATAAGCAAAT+CGG | + | chr3_2:51283343-51283362 | None:intergenic | 30.0% | |
| AATCATCCATAACAAGATTG+CGG | - | chr3_2:51283387-51283406 | Msa0409960:intron | 30.0% | |
| !! | TTTCTGCGCAAATTTTGTTT+CGG | - | chr3_2:51283428-51283447 | Msa0409960:intron | 30.0% |
| !! | TTCTGCTTCTTGTTTGATTT+TGG | - | chr3_2:51283519-51283538 | Msa0409960:intron | 30.0% |
| AACATGTCAGAAAGATTAAC+AGG | - | chr3_2:51283564-51283583 | Msa0409960:intron | 30.0% | |
| ACATAATCATCTGGACTATT+AGG | + | chr3_2:51283651-51283670 | None:intergenic | 30.0% | |
| !! | AAAAGTGCCTTAAAAGACAA+AGG | + | chr3_2:51283835-51283854 | None:intergenic | 30.0% |
| !! | TCAAATGATAATGGTATCAC+AGG | + | chr3_2:51284077-51284096 | None:intergenic | 30.0% |
| TGTGATACCATTATCATTTG+AGG | - | chr3_2:51284076-51284095 | Msa0409960:intron | 30.0% | |
| GTGATACCATTATCATTTGA+GGG | - | chr3_2:51284077-51284096 | Msa0409960:intron | 30.0% | |
| ATTACTCCCTCAAATGATAA+TGG | + | chr3_2:51284086-51284105 | None:intergenic | 30.0% | |
| TTAAAATATGACCTGATAGC+GGG | - | chr3_2:51284179-51284198 | Msa0409960:intron | 30.0% | |
| ! | TATTGTAGGTTTGCTTTACT+AGG | - | chr3_2:51284482-51284501 | Msa0409960:intron | 30.0% |
| ! | AACTATTGATGACTTAAGCT+TGG | + | chr3_2:51284741-51284760 | None:intergenic | 30.0% |
| AAGCTTAAGTCATCAATAGT+TGG | - | chr3_2:51284740-51284759 | Msa0409960:intron | 30.0% | |
| CTAACATCTTACAAACTACA+TGG | + | chr3_2:51284801-51284820 | None:intergenic | 30.0% | |
| AGAGATCTTTGCTTGATATC+AGG | + | chr3_2:51282244-51282263 | None:intergenic | 35.0% | |
| AGTTGTTACATAAGCTCTCT+AGG | + | chr3_2:51282606-51282625 | None:intergenic | 35.0% | |
| CTCAAGTGTGGTTAAATTTG+AGG | - | chr3_2:51282870-51282889 | Msa0409960:intron | 35.0% | |
| !!! | TGTCTTGCTGACTTACTTTT+AGG | - | chr3_2:51282911-51282930 | Msa0409960:intron | 35.0% |
| ! | TCTGTTCCGATTTGCTTATA+TGG | - | chr3_2:51283334-51283353 | Msa0409960:intron | 35.0% |
| ATCATCCATAACAAGATTGC+GGG | - | chr3_2:51283388-51283407 | Msa0409960:intron | 35.0% | |
| ! | TTTTTCCCGCAATCTTGTTA+TGG | + | chr3_2:51283396-51283415 | None:intergenic | 35.0% |
| !! | GATGGAAGCACTATAAACTT+TGG | - | chr3_2:51283450-51283469 | Msa0409960:intron | 35.0% |
| TTTGGATTATAGGATACGGT+TGG | - | chr3_2:51283468-51283487 | Msa0409960:intron | 35.0% | |
| !! | TGCTTCTTGTTTGATTTTGG+TGG | - | chr3_2:51283522-51283541 | Msa0409960:intron | 35.0% |
| GCAATTGGAACATAATCATC+TGG | + | chr3_2:51283660-51283679 | None:intergenic | 35.0% | |
| ! | TCCATGTCCTTTGTCTTTTA+AGG | - | chr3_2:51283825-51283844 | Msa0409960:intron | 35.0% |
| TTGTTAAGTGAGTCATTAGC+AGG | - | chr3_2:51283904-51283923 | Msa0409960:intron | 35.0% | |
| ! | TTAACATTTTTCCCGCTATC+AGG | + | chr3_2:51284193-51284212 | None:intergenic | 35.0% |
| !!! | CTTTTTTGTACAACACAGTC+AGG | + | chr3_2:51284317-51284336 | None:intergenic | 35.0% |
| TTTGCTTTACTAGGATGACA+GGG | - | chr3_2:51284491-51284510 | Msa0409960:intron | 35.0% | |
| !! | AAGATTTTGATGACCTGTTG+TGG | + | chr3_2:51284569-51284588 | None:intergenic | 35.0% |
| ! | CTTTGAATCATATTTGAGGC+CGG | + | chr3_2:51284654-51284673 | None:intergenic | 35.0% |
| TTAAGAAACTAGAACGGAGA+AGG | + | chr3_2:51284689-51284708 | None:intergenic | 35.0% | |
| CTCCTCTTAAGAAACTAGAA+CGG | + | chr3_2:51284695-51284714 | None:intergenic | 35.0% | |
| TGGTTGTAGTTAAGATTGGG+AGG | - | chr3_2:51282194-51282213 | Msa0409960:three_prime_UTR | 40.0% | |
| !!! | GCAAAGATCTCTGCTTTTTC+AGG | - | chr3_2:51282252-51282271 | Msa0409960:three_prime_UTR | 40.0% |
| ACTGAAACTTAGGCTGTGTT+TGG | - | chr3_2:51282445-51282464 | Msa0409960:CDS | 40.0% | |
| TTCACTTGGACATGTCACAA+TGG | + | chr3_2:51282801-51282820 | None:intergenic | 40.0% | |
| ACTTCTCTTACTCTCAAGTG+TGG | - | chr3_2:51282858-51282877 | Msa0409960:intron | 40.0% | |
| !! | TGTTTTGAATGATTCAGCGC+AGG | - | chr3_2:51283052-51283071 | Msa0409960:intron | 40.0% |
| !! | GTTTTGAATGATTCAGCGCA+GGG | - | chr3_2:51283053-51283072 | Msa0409960:intron | 40.0% |
| ! | TTTTGAATGATTCAGCGCAG+GGG | - | chr3_2:51283054-51283073 | Msa0409960:intron | 40.0% |
| !! | TGCGCAAATTTTGTTTCGGA+TGG | - | chr3_2:51283432-51283451 | Msa0409960:intron | 40.0% |
| TCCAATTGCATGCTTAGCTT+AGG | - | chr3_2:51283671-51283690 | Msa0409960:intron | 40.0% | |
| CATGCTTAGCTTAGGCTATT+TGG | - | chr3_2:51283679-51283698 | Msa0409960:intron | 40.0% | |
| GCCTTAAAAGACAAAGGACA+TGG | + | chr3_2:51283829-51283848 | None:intergenic | 40.0% | |
| TAAGTGAGTCATTAGCAGGA+AGG | - | chr3_2:51283908-51283927 | Msa0409960:intron | 40.0% | |
| AAGTGAGTCATTAGCAGGAA+GGG | - | chr3_2:51283909-51283928 | Msa0409960:intron | 40.0% | |
| ! | CACATGAAGTGCTTGAAACT+TGG | + | chr3_2:51284006-51284025 | None:intergenic | 40.0% |
| CGCTTACGCTATGTAAAAGT+AGG | + | chr3_2:51284217-51284236 | None:intergenic | 40.0% | |
| TGTAGTGTGTGTTTCCTGTT+GGG | - | chr3_2:51284271-51284290 | Msa0409960:intron | 40.0% | |
| ! | TTGGGTGTTGTGTAAACCAA+AGG | - | chr3_2:51284289-51284308 | Msa0409960:intron | 40.0% |
| GTTTGCTTTACTAGGATGAC+AGG | - | chr3_2:51284490-51284509 | Msa0409960:intron | 40.0% | |
| !! | CTCCGTTCTAGTTTCTTAAG+AGG | - | chr3_2:51284690-51284709 | Msa0409960:intron | 40.0% |
| TTGTTGCAAGAGTGAGGCTT+AGG | - | chr3_2:51282682-51282701 | Msa0409960:intron | 45.0% | |
| !! | GTAGACCTACTTTTCGGTTG+AGG | - | chr3_2:51282943-51282962 | Msa0409960:intron | 45.0% |
| CACATCCTCAACCGAAAAGT+AGG | + | chr3_2:51282951-51282970 | None:intergenic | 45.0% | |
| GCCTAAGCTAAGCATGCAAT+TGG | + | chr3_2:51283675-51283694 | None:intergenic | 45.0% | |
| GCTTAGCTTAGGCTATTTGG+AGG | - | chr3_2:51283682-51283701 | Msa0409960:intron | 45.0% | |
| TTGCAAGGTCTGAAGAGAAG+TGG | + | chr3_2:51284047-51284066 | None:intergenic | 45.0% | |
| ACTTCTCTTCAGACCTTGCA+AGG | - | chr3_2:51284046-51284065 | Msa0409960:intron | 45.0% | |
| !! | ATCACAGGAAGTACCTTGCA+AGG | + | chr3_2:51284062-51284081 | None:intergenic | 45.0% |
| GTGTAGTGTGTGTTTCCTGT+TGG | - | chr3_2:51284270-51284289 | Msa0409960:intron | 45.0% | |
| TGGTTTACACAACACCCAAC+AGG | + | chr3_2:51284288-51284307 | None:intergenic | 45.0% | |
| ACAACACAGTCAGGATCCTT+TGG | + | chr3_2:51284308-51284327 | None:intergenic | 45.0% | |
| GATGACCTGTTGTGGATTCA+TGG | + | chr3_2:51284561-51284580 | None:intergenic | 45.0% | |
| ATCTTGTAGACGAGTGACAG+TGG | - | chr3_2:51284584-51284603 | Msa0409960:intron | 45.0% | |
| GAGCACCATGAATCCACAAC+AGG | - | chr3_2:51284553-51284572 | Msa0409960:intron | 50.0% | |
| ACAACCGCTGAAGTGTGTAC+CGG | - | chr3_2:51284632-51284651 | Msa0409960:intron | 50.0% | |
| GAGGCCGGTACACACTTCAG+CGG | + | chr3_2:51284639-51284658 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 51282193 | 51284852 | 51282193 | ID=Msa0409960;Name=Msa0409960 |
| chr3_2 | mRNA | 51282193 | 51284852 | 51282193 | ID=Msa0409960-mRNA-1;Parent=Msa0409960;Name=Msa0409960-mRNA-1;_AED=0.24;_eAED=0.25;_QI=0|0|0.33|0.66|0|0.66|3|252|65 |
| chr3_2 | exon | 51282193 | 51282555 | 51282193 | ID=Msa0409960-mRNA-1:exon:10117;Parent=Msa0409960-mRNA-1 |
| chr3_2 | exon | 51283059 | 51283064 | 51283059 | ID=Msa0409960-mRNA-1:exon:10116;Parent=Msa0409960-mRNA-1 |
| chr3_2 | exon | 51284772 | 51284852 | 51284772 | ID=Msa0409960-mRNA-1:exon:10115;Parent=Msa0409960-mRNA-1 |
| chr3_2 | CDS | 51284772 | 51284852 | 51284772 | ID=Msa0409960-mRNA-1:cds;Parent=Msa0409960-mRNA-1 |
| chr3_2 | CDS | 51283059 | 51283064 | 51283059 | ID=Msa0409960-mRNA-1:cds;Parent=Msa0409960-mRNA-1 |
| chr3_2 | CDS | 51282445 | 51282555 | 51282445 | ID=Msa0409960-mRNA-1:cds;Parent=Msa0409960-mRNA-1 |
| chr3_2 | three_prime_UTR | 51282193 | 51282444 | 51282193 | ID=Msa0409960-mRNA-1:three_prime_utr;Parent=Msa0409960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
