AlfalfaGEDB | Msa0411730

M. sativa cultivar zhongmu-4 / Msa0411730

KEGG: K14963
GO: GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0411730	XP_040995868.1	92.308	78	6	0	1	78	31	108	1.25e-42	144

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0411730	sp\|Q10NY2\|TPR3_ORYSJ	91.139	79	7	0	1	79	100	178	6.70e-42	147

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0411730	A0A2I0HSB2	92.308	78	6	0	1	78	100	177	1.31e-42	145

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0411730	Msa0679300	0.808962	2.393698e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0411730	MtrunA17_Chr4g0003011	93.671	79	5	0	1	79	100	178	2.46e-44	151
Msa0411730	MtrunA17_Chr2g0333311	92.405	79	6	0	1	79	100	178	1.48e-43	149
Msa0411730	MtrunA17_Chr4g0064101	86.076	79	11	0	1	79	100	178	8.39e-41	141
Msa0411730	MtrunA17_Chr7g0271991	82.278	79	14	0	1	79	100	178	7.97e-39	135
Msa0411730	MtrunA17_Chr1g0192431	81.013	79	15	0	1	79	100	178	1.91e-38	134
Msa0411730	MtrunA17_Chr4g0067791	82.278	79	14	0	1	79	100	178	2.10e-37	131
Msa0411730	MtrunA17_Chr2g0295261	46.835	79	42	0	1	79	31	109	5.49e-20	79.7
Msa0411730	MtrunA17_Chr1g0149531	46.753	77	41	0	1	77	34	110	5.59e-20	77.0
Msa0411730	MtrunA17_Chr2g0310451	44.304	79	44	0	1	79	76	154	8.37e-20	79.7
Msa0411730	MtrunA17_Chr2g0295291	46.154	78	30	1	1	78	35	100	4.19e-16	66.6
Msa0411730	MtrunA17_Chr1g0153401	39.744	78	47	0	1	78	102	179	5.63e-14	63.2
Msa0411730	MtrunA17_Chr2g0295351	50.943	53	26	0	26	78	93	145	9.45e-12	58.5

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0411730	AT1G15750.3	89.873	79	8	1	79	100	178	9.38e-43	147
Msa0411730	AT1G15750.1	89.873	79	8	1	79	100	178	9.38e-43	147
Msa0411730	AT1G15750.2	89.873	79	8	1	79	100	178	9.38e-43	147
Msa0411730	AT1G15750.4	89.873	79	8	1	79	100	178	9.38e-43	147
Msa0411730	AT1G80490.1	88.608	79	9	1	79	100	178	1.24e-41	144
Msa0411730	AT1G80490.3	88.608	79	9	1	79	100	178	1.24e-41	144
Msa0411730	AT1G80490.2	88.608	79	9	1	79	100	178	1.24e-41	144
Msa0411730	AT3G15880.3	84.810	79	12	1	79	100	178	7.99e-40	139
Msa0411730	AT3G15880.1	84.810	79	12	1	79	100	178	8.07e-40	139
Msa0411730	AT3G15880.4	84.810	79	12	1	79	100	178	8.07e-40	139
Msa0411730	AT3G15880.2	84.810	79	12	1	79	100	178	8.07e-40	139
Msa0411730	AT3G16830.1	82.278	79	14	1	79	100	178	5.05e-38	134
Msa0411730	AT5G27030.1	79.747	79	16	1	79	100	178	7.09e-38	133
Msa0411730	AT5G27030.2	79.747	79	16	1	79	100	178	8.37e-38	133

Find 9 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAATTTGTCAGGAAATAA+TGG	0.201777	3_2:+54213113	None:intergenic
AATGAACAGTTGTCAAAGTA+TGG	0.361823	3_2:-54213197	Msa0411730:CDS
TTAGGAAATTGCAATTTGTC+AGG	0.408882	3_2:+54213104	None:intergenic
GTTTAATGAAGAATTGTTCA+AGG	0.447430	3_2:-54213341	Msa0411730:CDS
TTTGTCAGGAAATAATGGAT+TGG	0.495789	3_2:+54213118	None:intergenic
GTCTGCGCGGGCAGTCATGT+TGG	0.522666	3_2:-54213165	Msa0411730:CDS
AATCGCACAGCTATTGACAT+TGG	0.587279	3_2:-54213317	Msa0411730:CDS
TGGTGATACTAAGTCTGCGC+GGG	0.592019	3_2:-54213177	Msa0411730:CDS
ATGGTGATACTAAGTCTGCG+CGG	0.743006	3_2:-54213178	Msa0411730:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTTAATGAAGAATTGTTCA+AGG	-	chr3_2:54213081-54213100	Msa0411730:CDS	25.0%
!!	TATTGACATTGGAGAATTTT+AGG	-	chr3_2:54213116-54213135	Msa0411730:CDS	25.0%
!	ACATCTTACAAACTGAAAAT+AGG	+	chr3_2:54213175-54213194	None:intergenic	25.0%
!	TAATAATTTCCCAAACTCAA+TGG	+	chr3_2:54213200-54213219	None:intergenic	25.0%
!	ATTGAGTTTGGGAAATTATT+AGG	-	chr3_2:54213199-54213218	Msa0411730:CDS	25.0%
!	TTGAGTTTGGGAAATTATTA+GGG	-	chr3_2:54213200-54213219	Msa0411730:CDS	25.0%
!!!	AACTTTGAGTTTTTTAGGTT+AGG	+	chr3_2:54213339-54213358	None:intergenic	25.0%
!	ACCTAAAAAACTCAAAGTTA+AGG	-	chr3_2:54213340-54213359	Msa0411730:CDS	25.0%
!!!	TCCTTAACTTTGAGTTTTTT+AGG	+	chr3_2:54213344-54213363	None:intergenic	25.0%
	AATGAACAGTTGTCAAAGTA+TGG	-	chr3_2:54213225-54213244	Msa0411730:intron	30.0%
!!	TTTGTCAGGAAATAATGGAT+TGG	+	chr3_2:54213307-54213326	None:intergenic	30.0%
	TGCAATTTGTCAGGAAATAA+TGG	+	chr3_2:54213312-54213331	None:intergenic	30.0%
	TTAGGAAATTGCAATTTGTC+AGG	+	chr3_2:54213321-54213340	None:intergenic	30.0%
	TAAGATGTAGCCATTGAGTT+TGG	-	chr3_2:54213187-54213206	Msa0411730:CDS	35.0%
	AAGATGTAGCCATTGAGTTT+GGG	-	chr3_2:54213188-54213207	Msa0411730:CDS	35.0%
	AATCGCACAGCTATTGACAT+TGG	-	chr3_2:54213105-54213124	Msa0411730:CDS	40.0%
	ATGGTGATACTAAGTCTGCG+CGG	-	chr3_2:54213244-54213263	Msa0411730:intron	45.0%
	TGGTGATACTAAGTCTGCGC+GGG	-	chr3_2:54213245-54213264	Msa0411730:intron	50.0%
	GTCTGCGCGGGCAGTCATGT+TGG	-	chr3_2:54213257-54213276	Msa0411730:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	54213061	54213383	54213061	ID=Msa0411730;Name=Msa0411730
chr3_2	mRNA	54213061	54213383	54213061	ID=Msa0411730-mRNA-1;Parent=Msa0411730;Name=Msa0411730-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|0\|0\|2\|0\|79
chr3_2	exon	54213307	54213383	54213307	ID=Msa0411730-mRNA-1:exon:11002;Parent=Msa0411730-mRNA-1
chr3_2	exon	54213061	54213223	54213061	ID=Msa0411730-mRNA-1:exon:11001;Parent=Msa0411730-mRNA-1
chr3_2	CDS	54213307	54213383	54213307	ID=Msa0411730-mRNA-1:cds;Parent=Msa0411730-mRNA-1
chr3_2	CDS	54213061	54213223	54213061	ID=Msa0411730-mRNA-1:cds;Parent=Msa0411730-mRNA-1

Gene Sequence	>Msa0411730 GATTTGAAAGCGTTTGCTACGTTTAATGAAGAATTGTTCAAGGAAATCGCACAGCTATTGACATTGGAGAATTTTAGGGAAAATGAACAGTTGTCAAAGTATGGTGATACTAAGTCTGCGCGGGCAGTCATGTTGGTTGAGCTTAAAAAGCTCATTGAAGCCAATCCATTATTTCCTGACAAATTGCAATTTCCTAACCTAAAAAACTCAAAGTTAAGGACACTCATCAATCAAAGTTAA
Protein sequence	>Msa0411730 DLKAFATFNEELFKEIAQLLTLENFRENEQLSKYGDTKSARAVMLVELKKLIEANPLFPDKLQFPNLKNSKLRTLINQS*