AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414400 MtrunA17_Chr3g0107381 93.213 221 9 1 1 215 1 221 2.53e-153 424
Msa0414400 MtrunA17_Chr3g0110131 53.361 238 87 4 1 215 1 237 1.83e-74 225
Msa0414400 MtrunA17_Chr8g0351751 48.387 248 90 8 1 215 1 243 1.05e-62 195
Msa0414400 MtrunA17_Chr3g0107461 52.174 161 76 1 1 160 1 161 7.24e-56 175
Msa0414400 MtrunA17_Chr4g0014321 50.641 156 76 1 1 155 1 156 2.11e-53 171
Msa0414400 MtrunA17_Chr3g0107331 48.366 153 79 0 3 155 2 154 2.31e-51 163
Msa0414400 MtrunA17_Chr4g0013041 50.649 154 73 2 1 153 1 152 2.88e-51 164
Msa0414400 MtrunA17_Chr4g0014031 43.172 227 113 5 1 214 1 224 2.31e-50 163
Msa0414400 MtrunA17_Chr4g0014121 52.866 157 73 1 1 156 29 185 8.66e-49 157
Msa0414400 MtrunA17_Chr4g0014131 53.896 154 70 1 1 153 1 154 1.97e-48 158
Msa0414400 MtrunA17_Chr5g0432691 43.125 160 91 0 1 160 1 160 4.78e-47 152
Msa0414400 MtrunA17_Chr2g0296831 59.836 122 49 0 1 122 1 122 1.05e-46 150
Msa0414400 MtrunA17_Chr2g0283941 43.956 182 96 3 1 181 1 177 1.09e-46 154
Msa0414400 MtrunA17_Chr4g0031421 52.414 145 64 3 29 171 1 142 9.26e-44 143
Msa0414400 MtrunA17_Chr3g0107351 42.484 153 88 0 3 155 2 154 9.58e-43 141
Msa0414400 MtrunA17_Chr3g0107361 41.875 160 93 0 3 162 2 161 8.66e-42 139
Msa0414400 MtrunA17_Chr5g0420351 44.805 154 84 1 1 154 1 153 2.86e-41 137
Msa0414400 MtrunA17_Chr3g0105791 49.306 144 70 2 29 171 1 142 5.14e-41 136
Msa0414400 MtrunA17_Chr3g0107341 39.873 158 95 0 3 160 2 159 1.20e-39 133
Msa0414400 MtrunA17_Chr2g0296861 44.937 158 87 0 1 158 1 158 6.10e-37 126
Msa0414400 MtrunA17_Chr7g0218151 47.059 153 80 1 1 153 1 152 9.06e-36 123
Msa0414400 MtrunA17_Chr4g0012761 45.752 153 82 1 1 153 1 152 4.47e-34 119
Msa0414400 MtrunA17_Chr1g0197461 38.816 152 92 1 3 154 2 152 4.47e-34 119
Msa0414400 MtrunA17_Chr1g0188681 42.484 153 87 1 1 153 1 152 8.15e-34 118
Msa0414400 MtrunA17_Chr1g0197261 37.013 154 96 1 1 154 1 153 8.27e-34 118
Msa0414400 MtrunA17_Chr1g0193311 44.805 154 84 1 1 154 1 153 1.40e-33 118
Msa0414400 MtrunA17_Chr3g0106421 39.865 148 86 2 29 173 1 148 1.47e-32 117
Msa0414400 MtrunA17_Chr1g0188661 43.791 153 85 1 1 153 1 152 2.90e-32 114
Msa0414400 MtrunA17_Chr3g0106501 45.669 127 68 1 29 154 1 127 4.59e-31 111
Msa0414400 MtrunA17_Chr1g0197321 35.484 155 98 2 1 154 1 154 1.88e-29 107
Msa0414400 MtrunA17_Chr1g0188651 43.791 153 83 2 1 153 1 150 4.62e-29 106
Msa0414400 MtrunA17_Chr4g0008701 34.146 164 104 2 1 161 1 163 1.05e-23 98.2
Msa0414400 MtrunA17_Chr7g0267601 31.098 164 109 2 1 161 1 163 2.82e-22 94.0
Msa0414400 MtrunA17_Chr3g0128721 32.520 123 83 0 3 125 6 128 1.21e-19 86.7
Msa0414400 MtrunA17_Chr6g0457851 25.625 160 105 5 1 154 11 162 1.19e-15 74.3
Msa0414400 MtrunA17_Chr2g0296851 49.383 81 40 1 74 153 1 81 4.36e-15 68.2
Msa0414400 MtrunA17_Chr1g0212151 28.369 141 87 5 1 135 1 133 9.61e-15 72.0
Msa0414400 MtrunA17_Chr6g0449851 25.778 225 139 9 1 215 1 207 3.78e-14 70.1
Msa0414400 MtrunA17_Chr4g0022641 25.444 169 111 5 1 164 1 159 3.26e-13 67.8
Msa0414400 MtrunA17_Chr6g0449861 29.204 113 73 3 1 107 9 120 1.19e-12 65.9
Msa0414400 MtrunA17_Chr4g0059631 30.147 136 89 4 1 131 1 135 1.21e-12 65.1
Msa0414400 MtrunA17_Chr1g0212471 26.437 174 113 3 1 168 1 165 2.87e-12 63.2
Msa0414400 MtrunA17_Chr4g0061691 30.178 169 100 4 1 165 1 155 1.18e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414400 AT5G48670.1 57.516 153 65 0 1 153 1 153 1.15e-53 175
Msa0414400 AT3G05860.2 40.299 201 112 3 1 195 1 199 8.99e-42 140
Msa0414400 AT3G05860.1 41.361 191 104 3 1 185 1 189 1.08e-40 139
Msa0414400 AT3G05860.3 41.848 184 99 3 1 178 1 182 7.36e-40 137
Msa0414400 AT1G65300.1 41.060 151 89 0 3 153 2 152 1.60e-37 132
Msa0414400 AT5G27810.1 60.215 93 37 0 29 121 1 93 1.72e-35 122
Msa0414400 AT5G26650.1 40.667 150 89 0 4 153 2 151 2.70e-35 128
Msa0414400 AT1G65330.1 39.735 151 91 0 3 153 2 152 7.44e-35 125
Msa0414400 AT1G31630.1 38.816 152 90 3 3 153 2 151 5.73e-34 124
Msa0414400 AT1G31640.1 40.000 150 89 1 3 152 2 150 4.70e-33 124
Msa0414400 AT5G27960.1 38.667 150 92 0 4 153 2 151 7.59e-33 120
Msa0414400 AT5G26630.1 41.176 153 90 0 1 153 1 153 1.41e-32 117
Msa0414400 AT2G28700.1 36.667 150 93 2 1 149 1 149 1.43e-28 109
Msa0414400 AT1G22590.2 36.025 161 94 2 1 158 1 155 2.93e-26 99.8
Msa0414400 AT5G06500.1 29.714 175 119 2 1 175 1 171 4.27e-23 93.6
Msa0414400 AT5G26580.1 33.333 153 88 1 1 153 1 139 1.08e-22 94.4
Msa0414400 AT2G40210.1 42.188 128 74 0 1 128 1 128 1.84e-22 94.0
Msa0414400 AT2G15660.1 29.412 119 77 2 38 153 31 145 5.66e-12 63.5
Msa0414400 AT5G55690.1 35.714 98 56 2 1 91 1 98 1.73e-11 62.4
Msa0414400 AT5G55690.2 35.714 98 56 2 1 91 1 98 1.73e-11 62.4

Find 58 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTGTGCCACATGGTTTC+TGG 0.169034 3_2:-59900449 Msa0414400:CDS
TCCCCTCCTCTTTGTGTCAC+TGG 0.360448 3_2:+59901701 None:intergenic
CTCCAGTCACATTTCCAGCT+TGG 0.368302 3_2:+59900577 None:intergenic
CAGAGGTTTGGCCTTCGCCC+TGG 0.370238 3_2:-59900905 Msa0414400:CDS
TCAATTGTTGAATTTATGTT+TGG 0.370267 3_2:-59900357 Msa0414400:three_prime_UTR
AAATGTGACTGGAGGGGAGC+AGG 0.397749 3_2:-59900568 Msa0414400:CDS
AGAGGTTTGGCCTTCGCCCT+GGG 0.401660 3_2:-59900904 Msa0414400:CDS
TTCAAGAGACTTGTTGATCA+AGG 0.420086 3_2:+59900426 None:intergenic
AACAAAGGAATGACAACAAA+AGG 0.420798 3_2:-59900761 Msa0414400:CDS
GATGTGTTACCATTTGAAAA+TGG 0.433134 3_2:-59900474 Msa0414400:CDS
GGCCAATGCTGCCTTTGCAT+TGG 0.439467 3_2:+59900525 None:intergenic
TTCGCCCTGGGGAGTGCAAA+AGG 0.444082 3_2:-59900892 Msa0414400:CDS
ACGATCCTCAACCAGAGGTT+TGG 0.446002 3_2:-59900917 Msa0414400:CDS
CACATCACCAATGTTGTTGT+TGG 0.473556 3_2:+59900491 None:intergenic
GACTGGAGGGGAGCAGGGAA+TGG 0.473606 3_2:-59900562 Msa0414400:CDS
GCTCTAGTTCGGGCAATCTC+CGG 0.474104 3_2:+59900856 None:intergenic
ATCCAATGCAAAGGCAGCAT+TGG 0.475495 3_2:-59900527 Msa0414400:CDS
AGAGAAAGAAATGAGTAAGA+AGG 0.475985 3_2:+59901773 None:intergenic
CAGGGCGAAGGCCAAACCTC+TGG 0.485317 3_2:+59900906 None:intergenic
CAATCAGTCTCAAGCTGAAG+AGG 0.487013 3_2:-59900610 Msa0414400:CDS
AATGTGTCCATGCTTAGAGT+TGG 0.496808 3_2:-59900310 Msa0414400:three_prime_UTR
AATGTGACTGGAGGGGAGCA+GGG 0.507720 3_2:-59900567 Msa0414400:CDS
AAAAGGTGCTGTCAAGGTTC+CGG 0.507965 3_2:-59900875 Msa0414400:CDS
CTTTGTGTCACTGGTGATGT+AGG 0.508282 3_2:+59901710 None:intergenic
AAGAGAGTTTCTTGAAGCAA+AGG 0.517056 3_2:-59900812 Msa0414400:CDS
ATGTTTCAATGCCTTAATGC+TGG 0.536723 3_2:-59900720 Msa0414400:CDS
CGCTGCCATGACTAGGAAGA+AGG 0.539960 3_2:-59901742 Msa0414400:exon
TTCTCAACGCTGCCATGACT+AGG 0.541469 3_2:-59901749 Msa0414400:exon
GATTTCACCAACAACAACAT+TGG 0.549412 3_2:-59900498 Msa0414400:CDS
ATGAAATCAGCACGCTCTGT+GGG 0.552209 3_2:-59900971 Msa0414400:CDS
AACCAAGCTGGAAATGTGAC+TGG 0.555977 3_2:-59900579 Msa0414400:CDS
AGAACTTGAAACAGATCGAT+AGG 0.558346 3_2:-59900638 Msa0414400:CDS
GAGTGCAAAAGGTGCTGTCA+AGG 0.560319 3_2:-59900881 Msa0414400:CDS
GAGGTTGTTCAAAACCAAGC+TGG 0.560495 3_2:-59900591 Msa0414400:CDS
GCTCACCTTCTTCCTAGTCA+TGG 0.576647 3_2:+59901737 None:intergenic
CACCAGTGACACAAAGAGGA+GGG 0.584600 3_2:-59901703 Msa0414400:CDS
AAGCTGGAAATGTGACTGGA+GGG 0.588063 3_2:-59900575 Msa0414400:CDS
GCAAAGGCAGCATTGGCCAA+TGG 0.595451 3_2:-59900520 Msa0414400:CDS
TTGTTGTTGGTGAAATCCAT+TGG 0.595560 3_2:+59900504 None:intergenic
GAAGGCCAAACCTCTGGTTG+AGG 0.596659 3_2:+59900912 None:intergenic
AAGAACAGCTGAAGAAACAA+AGG 0.602104 3_2:-59900776 Msa0414400:CDS
AAGGAATGACAACAAAAGGA+AGG 0.606288 3_2:-59900757 Msa0414400:CDS
TGTCACTGGTGATGTAGGCA+AGG 0.608234 3_2:+59901715 None:intergenic
AGCTGGAAATGTGACTGGAG+GGG 0.624312 3_2:-59900574 Msa0414400:CDS
GAGGTTTGGCCTTCGCCCTG+GGG 0.626406 3_2:-59900903 Msa0414400:CDS
ACCAGTGACACAAAGAGGAG+GGG 0.628101 3_2:-59901702 Msa0414400:CDS
ACATCACCAGTGACACAAAG+AGG 0.631331 3_2:-59901707 Msa0414400:CDS
CCTGTGTCCAACTCTAAGCA+TGG 0.631681 3_2:+59900303 None:intergenic
CAAGCTGGAAATGTGACTGG+AGG 0.651743 3_2:-59900576 Msa0414400:CDS
ATGATCTCAATGATATCTCA+TGG 0.651968 3_2:-59900671 Msa0414400:CDS
ACATTGCTGATCCAATGCAA+AGG 0.652826 3_2:-59900536 Msa0414400:CDS
TGAGATCATCCATGCCAACA+TGG 0.658598 3_2:+59900685 None:intergenic
AATGGTAACATTGTGCCACA+TGG 0.661986 3_2:-59900456 Msa0414400:CDS
CCATGCTTAGAGTTGGACAC+AGG 0.668027 3_2:-59900303 Msa0414400:three_prime_UTR
TCAAAACGATCCTCAACCAG+AGG 0.673462 3_2:-59900922 Msa0414400:CDS
GATGAAATCAGCACGCTCTG+TGG 0.675321 3_2:-59900972 Msa0414400:CDS
TCACCAGTGACACAAAGAGG+AGG 0.677630 3_2:-59901704 Msa0414400:CDS
GATCAAGGCCAGAAACCATG+TGG 0.733784 3_2:+59900441 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATAAAATAAAAATT+GGG + chr3_2:59900827-59900846 None:intergenic 0.0%
!! TATTTAAATAAAATAAAAAT+TGG + chr3_2:59900828-59900847 None:intergenic 0.0%
!! ATTTAAATAAAATAAAAATT+GGG + chr3_2:59900827-59900846 None:intergenic 0.0%
!! TATTTAAATAAAATAAAAAT+TGG + chr3_2:59900828-59900847 None:intergenic 0.0%
!!! TAGTTTTTATCATTTTTCAA+TGG + chr3_2:59900532-59900551 None:intergenic 15.0%
!!! TGAAATTATTTTTATCTCAT+TGG - chr3_2:59900895-59900914 Msa0414400:CDS 15.0%
!!! TAGTTTTTATCATTTTTCAA+TGG + chr3_2:59900532-59900551 None:intergenic 15.0%
!!! TGAAATTATTTTTATCTCAT+TGG - chr3_2:59900895-59900914 Msa0414400:CDS 15.0%
!! ACATTCAAGAAAAGAAAAAA+TGG - chr3_2:59900391-59900410 Msa0414400:three_prime_UTR 20.0%
!! AAATCTTTCACTTAATTTAG+TGG + chr3_2:59900776-59900795 None:intergenic 20.0%
!!! AAAGATTTCATTTTGTTGTT+TGG - chr3_2:59900788-59900807 Msa0414400:CDS 20.0%
!! TCAATTGTTGAATTTATGTT+TGG - chr3_2:59901712-59901731 Msa0414400:CDS 20.0%
!! ACATTCAAGAAAAGAAAAAA+TGG - chr3_2:59900391-59900410 Msa0414400:three_prime_UTR 20.0%
!! AAATCTTTCACTTAATTTAG+TGG + chr3_2:59900776-59900795 None:intergenic 20.0%
!!! AAAGATTTCATTTTGTTGTT+TGG - chr3_2:59900788-59900807 Msa0414400:CDS 20.0%
!! TCAATTGTTGAATTTATGTT+TGG - chr3_2:59901712-59901731 Msa0414400:CDS 20.0%
! AATGGTAATTACAACATGTA+TGG + chr3_2:59900514-59900533 None:intergenic 25.0%
! AGATCGATAAATTGAATCAA+TGG - chr3_2:59900607-59900626 Msa0414400:CDS 25.0%
! GATCGATAAATTGAATCAAT+GGG - chr3_2:59900608-59900627 Msa0414400:CDS 25.0%
! ATCGATAAATTGAATCAATG+GGG - chr3_2:59900609-59900628 Msa0414400:CDS 25.0%
! TTAGTGGCAAACAAATTTAT+AGG + chr3_2:59900760-59900779 None:intergenic 25.0%
!!! TTTTTATCTCATTGGTGTTT+CGG - chr3_2:59900903-59900922 Msa0414400:CDS 25.0%
! AATGATTGAAACAACAAAGT+AGG + chr3_2:59901029-59901048 None:intergenic 25.0%
!!! CTTGTTGTATTTTTTATACC+AGG - chr3_2:59901058-59901077 Msa0414400:intron 25.0%
!! ACAATGTTACCATTTTCAAA+TGG + chr3_2:59901607-59901626 None:intergenic 25.0%
! AATGGTAATTACAACATGTA+TGG + chr3_2:59900514-59900533 None:intergenic 25.0%
! AGATCGATAAATTGAATCAA+TGG - chr3_2:59900607-59900626 Msa0414400:CDS 25.0%
! GATCGATAAATTGAATCAAT+GGG - chr3_2:59900608-59900627 Msa0414400:CDS 25.0%
! ATCGATAAATTGAATCAATG+GGG - chr3_2:59900609-59900628 Msa0414400:CDS 25.0%
! TTAGTGGCAAACAAATTTAT+AGG + chr3_2:59900760-59900779 None:intergenic 25.0%
!!! TTTTTATCTCATTGGTGTTT+CGG - chr3_2:59900903-59900922 Msa0414400:CDS 25.0%
! AATGATTGAAACAACAAAGT+AGG + chr3_2:59901029-59901048 None:intergenic 25.0%
!!! CTTGTTGTATTTTTTATACC+AGG - chr3_2:59901058-59901077 Msa0414400:intron 25.0%
!! ACAATGTTACCATTTTCAAA+TGG + chr3_2:59901607-59901626 None:intergenic 25.0%
AGAGAAAGAAATGAGTAAGA+AGG + chr3_2:59900299-59900318 None:intergenic 30.0%
ATGCAAGATTGATATAGACA+TGG + chr3_2:59900449-59900468 None:intergenic 30.0%
!!! TTTCATTTTGTTGTTTGGCT+TGG - chr3_2:59900793-59900812 Msa0414400:CDS 30.0%
ATTTAACATGTTTGTCCCTT+TGG - chr3_2:59900849-59900868 Msa0414400:CDS 30.0%
!! TTTTATCTCATTGGTGTTTC+GGG - chr3_2:59900904-59900923 Msa0414400:CDS 30.0%
!!! TTTTTTGCTTTGCTCTAGTT+CGG + chr3_2:59901227-59901246 None:intergenic 30.0%
AACAAAGGAATGACAACAAA+AGG - chr3_2:59901308-59901327 Msa0414400:intron 30.0%
ATGATCTCAATGATATCTCA+TGG - chr3_2:59901398-59901417 Msa0414400:intron 30.0%
GATGTGTTACCATTTGAAAA+TGG - chr3_2:59901595-59901614 Msa0414400:intron 30.0%
!! AATCTGCTTTGTTGTGTTTT+AGG - chr3_2:59901676-59901695 Msa0414400:intron 30.0%
AGAGAAAGAAATGAGTAAGA+AGG + chr3_2:59900299-59900318 None:intergenic 30.0%
ATGCAAGATTGATATAGACA+TGG + chr3_2:59900449-59900468 None:intergenic 30.0%
!!! TTTCATTTTGTTGTTTGGCT+TGG - chr3_2:59900793-59900812 Msa0414400:CDS 30.0%
ATTTAACATGTTTGTCCCTT+TGG - chr3_2:59900849-59900868 Msa0414400:CDS 30.0%
!! TTTTATCTCATTGGTGTTTC+GGG - chr3_2:59900904-59900923 Msa0414400:CDS 30.0%
!!! TTTTTTGCTTTGCTCTAGTT+CGG + chr3_2:59901227-59901246 None:intergenic 30.0%
AACAAAGGAATGACAACAAA+AGG - chr3_2:59901308-59901327 Msa0414400:intron 30.0%
ATGATCTCAATGATATCTCA+TGG - chr3_2:59901398-59901417 Msa0414400:intron 30.0%
GATGTGTTACCATTTGAAAA+TGG - chr3_2:59901595-59901614 Msa0414400:intron 30.0%
!! AATCTGCTTTGTTGTGTTTT+AGG - chr3_2:59901676-59901695 Msa0414400:intron 30.0%
ACTTCAGTTTACGATGTTGT+GGG + chr3_2:59900663-59900682 None:intergenic 35.0%
AACTTCAGTTTACGATGTTG+TGG + chr3_2:59900664-59900683 None:intergenic 35.0%
TCAATCTTCTTGATCAAACC+TGG + chr3_2:59901079-59901098 None:intergenic 35.0%
!! TTTTTGCTTTGCTCTAGTTC+GGG + chr3_2:59901226-59901245 None:intergenic 35.0%
AAGAGAGTTTCTTGAAGCAA+AGG - chr3_2:59901257-59901276 Msa0414400:intron 35.0%
AAGAACAGCTGAAGAAACAA+AGG - chr3_2:59901293-59901312 Msa0414400:intron 35.0%
AAGGAATGACAACAAAAGGA+AGG - chr3_2:59901312-59901331 Msa0414400:intron 35.0%
ATGTTTCAATGCCTTAATGC+TGG - chr3_2:59901349-59901368 Msa0414400:intron 35.0%
CAAAAATCTGACCAGCATTA+AGG + chr3_2:59901363-59901382 None:intergenic 35.0%
AGAACTTGAAACAGATCGAT+AGG - chr3_2:59901431-59901450 Msa0414400:intron 35.0%
! TTGTTGTTGGTGAAATCCAT+TGG + chr3_2:59901568-59901587 None:intergenic 35.0%
GATTTCACCAACAACAACAT+TGG - chr3_2:59901571-59901590 Msa0414400:intron 35.0%
!! TTCAAGAGACTTGTTGATCA+AGG + chr3_2:59901646-59901665 None:intergenic 35.0%
ACTTCAGTTTACGATGTTGT+GGG + chr3_2:59900663-59900682 None:intergenic 35.0%
AACTTCAGTTTACGATGTTG+TGG + chr3_2:59900664-59900683 None:intergenic 35.0%
TCAATCTTCTTGATCAAACC+TGG + chr3_2:59901079-59901098 None:intergenic 35.0%
!! TTTTTGCTTTGCTCTAGTTC+GGG + chr3_2:59901226-59901245 None:intergenic 35.0%
AAGAGAGTTTCTTGAAGCAA+AGG - chr3_2:59901257-59901276 Msa0414400:intron 35.0%
AAGAACAGCTGAAGAAACAA+AGG - chr3_2:59901293-59901312 Msa0414400:intron 35.0%
AAGGAATGACAACAAAAGGA+AGG - chr3_2:59901312-59901331 Msa0414400:intron 35.0%
ATGTTTCAATGCCTTAATGC+TGG - chr3_2:59901349-59901368 Msa0414400:intron 35.0%
CAAAAATCTGACCAGCATTA+AGG + chr3_2:59901363-59901382 None:intergenic 35.0%
AGAACTTGAAACAGATCGAT+AGG - chr3_2:59901431-59901450 Msa0414400:intron 35.0%
! TTGTTGTTGGTGAAATCCAT+TGG + chr3_2:59901568-59901587 None:intergenic 35.0%
GATTTCACCAACAACAACAT+TGG - chr3_2:59901571-59901590 Msa0414400:intron 35.0%
!! TTCAAGAGACTTGTTGATCA+AGG + chr3_2:59901646-59901665 None:intergenic 35.0%
GTTCAAATCCCAGTTGTGAA+AGG - chr3_2:59900681-59900700 Msa0414400:CDS 40.0%
TATGAGCACCTTTCACAACT+GGG + chr3_2:59900692-59900711 None:intergenic 40.0%
ATATGAGCACCTTTCACAAC+TGG + chr3_2:59900693-59900712 None:intergenic 40.0%
! TCAGCTGTTCTTGAGCTTTT+TGG + chr3_2:59901285-59901304 None:intergenic 40.0%
! GGTCAGATTTTTGACCATGT+TGG - chr3_2:59901370-59901389 Msa0414400:intron 40.0%
! GATTTTTGACCATGTTGGCA+TGG - chr3_2:59901375-59901394 Msa0414400:intron 40.0%
ACATTGCTGATCCAATGCAA+AGG - chr3_2:59901533-59901552 Msa0414400:intron 40.0%
CACATCACCAATGTTGTTGT+TGG + chr3_2:59901581-59901600 None:intergenic 40.0%
AATGGTAACATTGTGCCACA+TGG - chr3_2:59901613-59901632 Msa0414400:intron 40.0%
AATGTGTCCATGCTTAGAGT+TGG - chr3_2:59901759-59901778 Msa0414400:five_prime_UTR 40.0%
GTTCAAATCCCAGTTGTGAA+AGG - chr3_2:59900681-59900700 Msa0414400:CDS 40.0%
TATGAGCACCTTTCACAACT+GGG + chr3_2:59900692-59900711 None:intergenic 40.0%
ATATGAGCACCTTTCACAAC+TGG + chr3_2:59900693-59900712 None:intergenic 40.0%
! TCAGCTGTTCTTGAGCTTTT+TGG + chr3_2:59901285-59901304 None:intergenic 40.0%
! GGTCAGATTTTTGACCATGT+TGG - chr3_2:59901370-59901389 Msa0414400:intron 40.0%
! GATTTTTGACCATGTTGGCA+TGG - chr3_2:59901375-59901394 Msa0414400:intron 40.0%
ACATTGCTGATCCAATGCAA+AGG - chr3_2:59901533-59901552 Msa0414400:intron 40.0%
CACATCACCAATGTTGTTGT+TGG + chr3_2:59901581-59901600 None:intergenic 40.0%
AATGGTAACATTGTGCCACA+TGG - chr3_2:59901613-59901632 Msa0414400:intron 40.0%
AATGTGTCCATGCTTAGAGT+TGG - chr3_2:59901759-59901778 Msa0414400:five_prime_UTR 40.0%
! CTTTGTGTCACTGGTGATGT+AGG + chr3_2:59900362-59900381 None:intergenic 45.0%
ACATCACCAGTGACACAAAG+AGG - chr3_2:59900362-59900381 Msa0414400:three_prime_UTR 45.0%
GGGAGTTGAGTCTAGCAAAA+TGG - chr3_2:59900629-59900648 Msa0414400:CDS 45.0%
CACACAGACGCTATTCCAAA+GGG + chr3_2:59900867-59900886 None:intergenic 45.0%
TCACACAGACGCTATTCCAA+AGG + chr3_2:59900868-59900887 None:intergenic 45.0%
ATGAAATCAGCACGCTCTGT+GGG - chr3_2:59901098-59901117 Msa0414400:intron 45.0%
TCAAAACGATCCTCAACCAG+AGG - chr3_2:59901147-59901166 Msa0414400:intron 45.0%
!! AAAAGGTGCTGTCAAGGTTC+CGG - chr3_2:59901194-59901213 Msa0414400:intron 45.0%
TGAGATCATCCATGCCAACA+TGG + chr3_2:59901387-59901406 None:intergenic 45.0%
CAATCAGTCTCAAGCTGAAG+AGG - chr3_2:59901459-59901478 Msa0414400:intron 45.0%
GAGGTTGTTCAAAACCAAGC+TGG - chr3_2:59901478-59901497 Msa0414400:intron 45.0%
AACCAAGCTGGAAATGTGAC+TGG - chr3_2:59901490-59901509 Msa0414400:intron 45.0%
AAGCTGGAAATGTGACTGGA+GGG - chr3_2:59901494-59901513 Msa0414400:intron 45.0%
ATCCAATGCAAAGGCAGCAT+TGG - chr3_2:59901542-59901561 Msa0414400:intron 45.0%
ACATTGTGCCACATGGTTTC+TGG - chr3_2:59901620-59901639 Msa0414400:intron 45.0%
! CTTTGTGTCACTGGTGATGT+AGG + chr3_2:59900362-59900381 None:intergenic 45.0%
ACATCACCAGTGACACAAAG+AGG - chr3_2:59900362-59900381 Msa0414400:three_prime_UTR 45.0%
GGGAGTTGAGTCTAGCAAAA+TGG - chr3_2:59900629-59900648 Msa0414400:CDS 45.0%
CACACAGACGCTATTCCAAA+GGG + chr3_2:59900867-59900886 None:intergenic 45.0%
TCACACAGACGCTATTCCAA+AGG + chr3_2:59900868-59900887 None:intergenic 45.0%
ATGAAATCAGCACGCTCTGT+GGG - chr3_2:59901098-59901117 Msa0414400:intron 45.0%
TCAAAACGATCCTCAACCAG+AGG - chr3_2:59901147-59901166 Msa0414400:intron 45.0%
!! AAAAGGTGCTGTCAAGGTTC+CGG - chr3_2:59901194-59901213 Msa0414400:intron 45.0%
TGAGATCATCCATGCCAACA+TGG + chr3_2:59901387-59901406 None:intergenic 45.0%
CAATCAGTCTCAAGCTGAAG+AGG - chr3_2:59901459-59901478 Msa0414400:intron 45.0%
GAGGTTGTTCAAAACCAAGC+TGG - chr3_2:59901478-59901497 Msa0414400:intron 45.0%
AACCAAGCTGGAAATGTGAC+TGG - chr3_2:59901490-59901509 Msa0414400:intron 45.0%
AAGCTGGAAATGTGACTGGA+GGG - chr3_2:59901494-59901513 Msa0414400:intron 45.0%
ATCCAATGCAAAGGCAGCAT+TGG - chr3_2:59901542-59901561 Msa0414400:intron 45.0%
ACATTGTGCCACATGGTTTC+TGG - chr3_2:59901620-59901639 Msa0414400:intron 45.0%
!! TTTAAATAAAATAAAAATTG+GGG + chr3_2:59900826-59900845 None:intergenic 5.0%
!! TTTAAATAAAATAAAAATTG+GGG + chr3_2:59900826-59900845 None:intergenic 5.0%
TTCTCAACGCTGCCATGACT+AGG - chr3_2:59900320-59900339 Msa0414400:three_prime_UTR 50.0%
GCTCACCTTCTTCCTAGTCA+TGG + chr3_2:59900335-59900354 None:intergenic 50.0%
! TGTCACTGGTGATGTAGGCA+AGG + chr3_2:59900357-59900376 None:intergenic 50.0%
TCACCAGTGACACAAAGAGG+AGG - chr3_2:59900365-59900384 Msa0414400:three_prime_UTR 50.0%
CACCAGTGACACAAAGAGGA+GGG - chr3_2:59900366-59900385 Msa0414400:three_prime_UTR 50.0%
ACCAGTGACACAAAGAGGAG+GGG - chr3_2:59900367-59900386 Msa0414400:three_prime_UTR 50.0%
GATGAAATCAGCACGCTCTG+TGG - chr3_2:59901097-59901116 Msa0414400:intron 50.0%
ACGATCCTCAACCAGAGGTT+TGG - chr3_2:59901152-59901171 Msa0414400:intron 50.0%
!! GAGTGCAAAAGGTGCTGTCA+AGG - chr3_2:59901188-59901207 Msa0414400:intron 50.0%
CTCCAGTCACATTTCCAGCT+TGG + chr3_2:59901495-59901514 None:intergenic 50.0%
CAAGCTGGAAATGTGACTGG+AGG - chr3_2:59901493-59901512 Msa0414400:intron 50.0%
AGCTGGAAATGTGACTGGAG+GGG - chr3_2:59901495-59901514 Msa0414400:intron 50.0%
GATCAAGGCCAGAAACCATG+TGG + chr3_2:59901631-59901650 None:intergenic 50.0%
CCTGTGTCCAACTCTAAGCA+TGG + chr3_2:59901769-59901788 None:intergenic 50.0%
CCATGCTTAGAGTTGGACAC+AGG - chr3_2:59901766-59901785 Msa0414400:five_prime_UTR 50.0%
TTCTCAACGCTGCCATGACT+AGG - chr3_2:59900320-59900339 Msa0414400:three_prime_UTR 50.0%
GCTCACCTTCTTCCTAGTCA+TGG + chr3_2:59900335-59900354 None:intergenic 50.0%
! TGTCACTGGTGATGTAGGCA+AGG + chr3_2:59900357-59900376 None:intergenic 50.0%
TCACCAGTGACACAAAGAGG+AGG - chr3_2:59900365-59900384 Msa0414400:three_prime_UTR 50.0%
CACCAGTGACACAAAGAGGA+GGG - chr3_2:59900366-59900385 Msa0414400:three_prime_UTR 50.0%
ACCAGTGACACAAAGAGGAG+GGG - chr3_2:59900367-59900386 Msa0414400:three_prime_UTR 50.0%
GATGAAATCAGCACGCTCTG+TGG - chr3_2:59901097-59901116 Msa0414400:intron 50.0%
ACGATCCTCAACCAGAGGTT+TGG - chr3_2:59901152-59901171 Msa0414400:intron 50.0%
!! GAGTGCAAAAGGTGCTGTCA+AGG - chr3_2:59901188-59901207 Msa0414400:intron 50.0%
CTCCAGTCACATTTCCAGCT+TGG + chr3_2:59901495-59901514 None:intergenic 50.0%
CAAGCTGGAAATGTGACTGG+AGG - chr3_2:59901493-59901512 Msa0414400:intron 50.0%
AGCTGGAAATGTGACTGGAG+GGG - chr3_2:59901495-59901514 Msa0414400:intron 50.0%
GATCAAGGCCAGAAACCATG+TGG + chr3_2:59901631-59901650 None:intergenic 50.0%
CCTGTGTCCAACTCTAAGCA+TGG + chr3_2:59901769-59901788 None:intergenic 50.0%
CCATGCTTAGAGTTGGACAC+AGG - chr3_2:59901766-59901785 Msa0414400:five_prime_UTR 50.0%
CGCTGCCATGACTAGGAAGA+AGG - chr3_2:59900327-59900346 Msa0414400:three_prime_UTR 55.0%
TCCCCTCCTCTTTGTGTCAC+TGG + chr3_2:59900371-59900390 None:intergenic 55.0%
GAAGGCCAAACCTCTGGTTG+AGG + chr3_2:59901160-59901179 None:intergenic 55.0%
! AGCACCTTTTGCACTCCCCA+GGG + chr3_2:59901184-59901203 None:intergenic 55.0%
! GCTCTAGTTCGGGCAATCTC+CGG + chr3_2:59901216-59901235 None:intergenic 55.0%
AAATGTGACTGGAGGGGAGC+AGG - chr3_2:59901501-59901520 Msa0414400:intron 55.0%
AATGTGACTGGAGGGGAGCA+GGG - chr3_2:59901502-59901521 Msa0414400:intron 55.0%
GGCCAATGCTGCCTTTGCAT+TGG + chr3_2:59901547-59901566 None:intergenic 55.0%
!! GCAAAGGCAGCATTGGCCAA+TGG - chr3_2:59901549-59901568 Msa0414400:intron 55.0%
CGCTGCCATGACTAGGAAGA+AGG - chr3_2:59900327-59900346 Msa0414400:three_prime_UTR 55.0%
TCCCCTCCTCTTTGTGTCAC+TGG + chr3_2:59900371-59900390 None:intergenic 55.0%
GAAGGCCAAACCTCTGGTTG+AGG + chr3_2:59901160-59901179 None:intergenic 55.0%
! AGCACCTTTTGCACTCCCCA+GGG + chr3_2:59901184-59901203 None:intergenic 55.0%
! GCTCTAGTTCGGGCAATCTC+CGG + chr3_2:59901216-59901235 None:intergenic 55.0%
AAATGTGACTGGAGGGGAGC+AGG - chr3_2:59901501-59901520 Msa0414400:intron 55.0%
AATGTGACTGGAGGGGAGCA+GGG - chr3_2:59901502-59901521 Msa0414400:intron 55.0%
GGCCAATGCTGCCTTTGCAT+TGG + chr3_2:59901547-59901566 None:intergenic 55.0%
!! GCAAAGGCAGCATTGGCCAA+TGG - chr3_2:59901549-59901568 Msa0414400:intron 55.0%
! AGAGGTTTGGCCTTCGCCCT+GGG - chr3_2:59901165-59901184 Msa0414400:intron 60.0%
! TTTTGCACTCCCCAGGGCGA+AGG + chr3_2:59901178-59901197 None:intergenic 60.0%
! TTCGCCCTGGGGAGTGCAAA+AGG - chr3_2:59901177-59901196 Msa0414400:intron 60.0%
! CAGCACCTTTTGCACTCCCC+AGG + chr3_2:59901185-59901204 None:intergenic 60.0%
! AGAGGTTTGGCCTTCGCCCT+GGG - chr3_2:59901165-59901184 Msa0414400:intron 60.0%
! TTTTGCACTCCCCAGGGCGA+AGG + chr3_2:59901178-59901197 None:intergenic 60.0%
! TTCGCCCTGGGGAGTGCAAA+AGG - chr3_2:59901177-59901196 Msa0414400:intron 60.0%
! CAGCACCTTTTGCACTCCCC+AGG + chr3_2:59901185-59901204 None:intergenic 60.0%
CAGGGCGAAGGCCAAACCTC+TGG + chr3_2:59901166-59901185 None:intergenic 65.0%
! CAGAGGTTTGGCCTTCGCCC+TGG - chr3_2:59901164-59901183 Msa0414400:intron 65.0%
! GAGGTTTGGCCTTCGCCCTG+GGG - chr3_2:59901166-59901185 Msa0414400:intron 65.0%
GACTGGAGGGGAGCAGGGAA+TGG - chr3_2:59901507-59901526 Msa0414400:intron 65.0%
CAGGGCGAAGGCCAAACCTC+TGG + chr3_2:59901166-59901185 None:intergenic 65.0%
! CAGAGGTTTGGCCTTCGCCC+TGG - chr3_2:59901164-59901183 Msa0414400:intron 65.0%
! GAGGTTTGGCCTTCGCCCTG+GGG - chr3_2:59901166-59901185 Msa0414400:intron 65.0%
GACTGGAGGGGAGCAGGGAA+TGG - chr3_2:59901507-59901526 Msa0414400:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 59900287 59901804 59900287 ID=Msa0414400;Name=Msa0414400
chr3_2 mRNA 59900287 59901804 59900287 ID=Msa0414400-mRNA-1;Parent=Msa0414400;Name=Msa0414400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=47|1|1|1|1|1|2|156|215
chr3_2 exon 59900287 59901011 59900287 ID=Msa0414400-mRNA-1:exon:12469;Parent=Msa0414400-mRNA-1
chr3_2 exon 59901679 59901804 59901679 ID=Msa0414400-mRNA-1:exon:12468;Parent=Msa0414400-mRNA-1
chr3_2 five_prime_UTR 59901758 59901804 59901758 ID=Msa0414400-mRNA-1:five_prime_utr;Parent=Msa0414400-mRNA-1
chr3_2 CDS 59901679 59901757 59901679 ID=Msa0414400-mRNA-1:cds;Parent=Msa0414400-mRNA-1
chr3_2 CDS 59900443 59901011 59900443 ID=Msa0414400-mRNA-1:cds;Parent=Msa0414400-mRNA-1
chr3_2 three_prime_UTR 59900287 59900442 59900287 ID=Msa0414400-mRNA-1:three_prime_utr;Parent=Msa0414400-mRNA-1
Gene Sequence

>Msa0414400

ATGACTAGGAAGAAGGTGAGCCTTGCCTACATCACCAGTGACACAAAGAGGAGGGGAACATTCAAGAAAAGAAAAAATGGTTTGATCAAGAAGATTGATGAAATCAGCACGCTCTGTGGGATCGAAGCGTGTGCAATCATCTTTGATCAAAACGATCCTCAACCAGAGGTTTGGCCTTCGCCCTGGGGAGTGCAAAAGGTGCTGTCAAGGTTCCGGAGATTGCCCGAACTAGAGCAAAGCAAAAAAATGTTCAATCAAGAGAGTTTCTTGAAGCAAAGGATCCAAAAAGCTCAAGAACAGCTGAAGAAACAAAGGAATGACAACAAAAGGAAGGAAATGACTCATCTGATGTTTCAATGCCTTAATGCTGGTCAGATTTTTGACCATGTTGGCATGGATGATCTCAATGATATCTCATGGTTGATTGATCAGAACTTGAAACAGATCGATAGGAAACTCAATCAGTCTCAAGCTGAAGAGGTTGTTCAAAACCAAGCTGGAAATGTGACTGGAGGGGAGCAGGGAATGGAGAACATTGCTGATCCAATGCAAAGGCAGCATTGGCCAATGGATTTCACCAACAACAACATTGGTGATGTGTTACCATTTGAAAATGGTAACATTGTGCCACATGGTTTCTGGCCTTGA
Protein sequence

>Msa0414400

MTRKKVSLAYITSDTKRRGTFKKRKNGLIKKIDEISTLCGIEACAIIFDQNDPQPEVWPSPWGVQKVLSRFRRLPELEQSKKMFNQESFLKQRIQKAQEQLKKQRNDNKRKEMTHLMFQCLNAGQIFDHVGMDDLNDISWLIDQNLKQIDRKLNQSQAEEVVQNQAGNVTGGEQGMENIADPMQRQHWPMDFTNNNIGDVLPFENGNIVPHGFWP*