AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414630 MtrunA17_Chr3g0107671 76.106 565 127 4 1 563 16 574 0.0 884
Msa0414630 MtrunA17_Chr6g0451531 65.785 567 182 6 1 563 18 576 0.0 747
Msa0414630 MtrunA17_Chr6g0451541 62.898 566 201 4 1 563 18 577 0.0 711
Msa0414630 MtrunA17_Chr6g0451561 61.498 574 195 10 1 563 16 574 0.0 672
Msa0414630 MtrunA17_Chr6g0451511 54.101 573 241 12 1 563 16 576 0.0 567
Msa0414630 MtrunA17_Chr3g0107661 71.585 183 47 3 2 183 17 195 4.89e-82 257
Msa0414630 MtrunA17_Chr3g0107651 72.464 138 36 1 267 404 1 136 1.42e-65 210
Msa0414630 MtrunA17_Chr8g0384621 27.108 498 263 17 120 535 132 611 2.17e-38 149
Msa0414630 MtrunA17_Chr3g0118251 25.414 362 206 16 197 529 274 600 2.57e-21 97.8
Msa0414630 MtrunA17_Chr3g0118541 23.729 354 215 12 199 529 287 608 3.05e-16 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414630 AT5G14930.2 35.924 579 290 20 1 557 15 534 2.43e-96 304
Msa0414630 AT3G52430.1 27.594 453 281 14 103 535 95 520 3.89e-39 150
Msa0414630 AT5G14930.3 37.946 224 115 7 1 213 15 225 5.35e-33 126
Msa0414630 AT3G48080.1 23.562 365 218 12 199 528 265 603 6.15e-13 72.0

Find 106 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGACCATTATAGCATTTA+AGG 0.089703 3_2:+60248410 Msa0414630:CDS
CTTCATATGTTTCCTTTCTT+TGG 0.160648 3_2:+60250790 Msa0414630:three_prime_UTR
CCTTCAACTCTTTGCATAAA+AGG 0.176393 3_2:-60250527 None:intergenic
GAAGGAGTATAAAGAGATTA+AGG 0.184976 3_2:+60250677 Msa0414630:CDS
GAATTTGAGGTGTATGTTTA+TGG 0.187587 3_2:+60250588 Msa0414630:CDS
CCATCTCACGTAGTTCTATT+TGG 0.208119 3_2:+60249243 Msa0414630:CDS
TGACTATAGATTCTTGCTTT+TGG 0.226808 3_2:+60250484 Msa0414630:CDS
GGACCTGAAACTATTGATTA+TGG 0.274388 3_2:+60249452 Msa0414630:CDS
CGGACGCCGTTGATTGTTAC+TGG 0.281265 3_2:+60249089 Msa0414630:CDS
ATTTCATTCAATTTCCTTGT+TGG 0.284935 3_2:-60250057 None:intergenic
AAGACTATGTTAATAATAAA+AGG 0.286162 3_2:+60250361 Msa0414630:CDS
ACCAGTACCTATATGCCTTT+TGG 0.305241 3_2:+60249332 Msa0414630:CDS
TTTCCATAATCAATAGTTTC+AGG 0.315617 3_2:-60249455 None:intergenic
TTTCGTACTCGATGGCTATA+TGG 0.317770 3_2:+60250273 Msa0414630:CDS
CAGTGTCTCTTCTCTCTCAT+TGG 0.318579 3_2:-60250553 None:intergenic
CTCCAACTGCTTGAAGTGTT+TGG 0.324055 3_2:-60250385 None:intergenic
GATTTCTTCTTCTGAACTTA+AGG 0.324062 3_2:+60248467 Msa0414630:CDS
GATAGATTAGGATGATTATA+TGG 0.335612 3_2:+60250816 Msa0414630:three_prime_UTR
AGTAACTGGTGAGCTTCTTA+TGG 0.336614 3_2:-60250197 None:intergenic
ACGATGGTGTTGGACTTTCT+TGG 0.337988 3_2:+60248361 Msa0414630:CDS
ATAAGAAGCTCACCAGTTAC+TGG 0.352381 3_2:+60250199 Msa0414630:CDS
AATTTGAGGTGTATGTTTAT+GGG 0.353338 3_2:+60250589 Msa0414630:CDS
ATTGTTACTGGGCATGGTCT+TGG 0.355599 3_2:+60249101 Msa0414630:CDS
CCAACTTGAATGGTACAAAA+AGG 0.362345 3_2:+60250095 Msa0414630:CDS
CGTCCACTCTGCATCACCTT+TGG 0.363380 3_2:+60249185 Msa0414630:CDS
ACCTTTGGTTCACCCCTTGT+TGG 0.372203 3_2:+60249200 Msa0414630:CDS
TTTCTTTGGTTTGATAGATT+AGG 0.374725 3_2:+60250804 Msa0414630:three_prime_UTR
TATATGGCGGGACTACTTAC+AGG 0.375910 3_2:+60250289 Msa0414630:CDS
TGAGAATGCAGAATCTATCT+TGG 0.379413 3_2:+60249388 Msa0414630:CDS
GAAGAAATTGTTTGAATTTG+AGG 0.385810 3_2:+60250575 Msa0414630:CDS
AACCATCTTCTCCCAGTAAC+TGG 0.386972 3_2:-60250211 None:intergenic
TATTCACTTACCTGCATTTG+TGG 0.395203 3_2:-60249602 None:intergenic
TGCAGCCTTAAATGCTATAA+TGG 0.407236 3_2:-60248415 None:intergenic
GTTCACCCCTTGTTGGAGAT+AGG 0.416824 3_2:+60249207 Msa0414630:CDS
GAATGAAATGAAGGTACATA+TGG 0.423812 3_2:+60250071 Msa0414630:CDS
TCAGAAGAAGAAATCAAATC+AGG 0.424430 3_2:-60248459 None:intergenic
GTTACTGGGCATGGTCTTGG+AGG 0.436822 3_2:+60249104 Msa0414630:CDS
AAATGTTCCAAAAGGCATAT+AGG 0.444177 3_2:-60249339 None:intergenic
AAGGAGTATAAAGAGATTAA+GGG 0.444783 3_2:+60250678 Msa0414630:CDS
GCAGTTGGAGGAGTGGTTGA+TGG 0.445794 3_2:+60250398 Msa0414630:CDS
CTAGTATACTTTGTAAGTCA+TGG 0.476826 3_2:+60248295 Msa0414630:CDS
AGATTCTGCATTCTCAAAAG+AGG 0.481140 3_2:-60249381 None:intergenic
CCGTTGATTGTTACTGGGCA+TGG 0.487392 3_2:+60249095 Msa0414630:CDS
GGTCAAGATTCACTAGCAAC+TGG 0.490730 3_2:+60249545 Msa0414630:CDS
GTAATGAAGCGCCTTGGAAG+TGG 0.498921 3_2:-60249302 None:intergenic
AGTTGAAATGAAGCCTCAAA+AGG 0.503138 3_2:+60250239 Msa0414630:CDS
GTCCAAACACTTCAAGCAGT+TGG 0.503403 3_2:+60250383 Msa0414630:CDS
AAGGAAATTGAATGAAATGA+AGG 0.505485 3_2:+60250062 Msa0414630:CDS
AACTGGCATTAGTTTGCAGT+TGG 0.510157 3_2:+60249562 Msa0414630:CDS
TCCAAAAGGCATATAGGTAC+TGG 0.510716 3_2:-60249333 None:intergenic
CACCAGTTACTGGGAGAAGA+TGG 0.512473 3_2:+60250209 Msa0414630:CDS
GAATCTAAGAATCAGCAAGC+AGG 0.512587 3_2:+60250117 Msa0414630:CDS
TACATATGGCCCAACTTGAA+TGG 0.515555 3_2:+60250085 Msa0414630:CDS
GGACGCCGTTGATTGTTACT+GGG 0.515744 3_2:+60249090 Msa0414630:CDS
CACAAAGCAGCTATATTCGT+TGG 0.519667 3_2:+60250652 Msa0414630:CDS
GAGCAATTCATAGATTGAAG+TGG 0.520110 3_2:+60250845 Msa0414630:three_prime_UTR
AAAATTGAGAAGTATGCTAC+AGG 0.520879 3_2:+60250747 Msa0414630:CDS
AGAATTGACACCACAAATGC+AGG 0.527465 3_2:+60249592 Msa0414630:CDS
TGTACTGCTTATATCACTTG+TGG 0.529588 3_2:-60250436 None:intergenic
GAACAGTGAAATCAATACTC+TGG 0.532043 3_2:+60249975 Msa0414630:CDS
ATTGTTTCAAACGATGGTGT+TGG 0.533442 3_2:+60248351 Msa0414630:CDS
TAATCTATCAAACCAAAGAA+AGG 0.534196 3_2:-60250802 None:intergenic
CACATGATAAATGTTCCAAA+AGG 0.540984 3_2:-60249347 None:intergenic
ATATCACTATTAAATAGCAT+TGG 0.545231 3_2:+60249149 Msa0414630:CDS
TTCTTCTTCTGAACTTAAGG+AGG 0.546105 3_2:+60248470 Msa0414630:CDS
TAGTAGTCAGCAATAGCTAA+AGG 0.551669 3_2:-60250324 None:intergenic
CAGCTATATTCGTTGGTGGA+AGG 0.560039 3_2:+60250659 Msa0414630:CDS
CAGATTTCAATATATCAAGT+TGG 0.560140 3_2:-60248587 None:intergenic
TAAGAAGCTCACCAGTTACT+GGG 0.561023 3_2:+60250200 Msa0414630:CDS
CCAAATAGAACTACGTGAGA+TGG 0.563081 3_2:-60249243 None:intergenic
TCCAACAAGGGGTGAACCAA+AGG 0.564114 3_2:-60249201 None:intergenic
GGACTTGTAATGAAGCGCCT+TGG 0.566613 3_2:-60249308 None:intergenic
GTATGTTTATGGGTTGCTCA+AGG 0.567939 3_2:+60250599 Msa0414630:CDS
AAAGATTCATCCTACAGAAG+AGG 0.568943 3_2:+60250022 Msa0414630:CDS
ACTTCAAGCAGTTGGAGGAG+TGG 0.569280 3_2:+60250391 Msa0414630:CDS
GTACTCGATGGCTATATGGC+GGG 0.571976 3_2:+60250277 Msa0414630:CDS
CAAATGACATCCTCTTCTGT+AGG 0.574147 3_2:-60250032 None:intergenic
AATCTAAGAATCAGCAAGCA+GGG 0.582487 3_2:+60250118 Msa0414630:CDS
AACACAGAAAGTCAAAGTCA+TGG 0.584990 3_2:-60248500 None:intergenic
GTAACAATCAACGGCGTCCG+TGG 0.585114 3_2:-60249086 None:intergenic
TAATGAAGCGCCTTGGAAGT+GGG 0.585676 3_2:-60249301 None:intergenic
GCAATCTCCTATCTCCAACA+AGG 0.587511 3_2:-60249214 None:intergenic
CAATCTCCTATCTCCAACAA+GGG 0.590929 3_2:-60249213 None:intergenic
CACACAGAGACCCACTTCCA+AGG 0.593153 3_2:+60249291 Msa0414630:CDS
ATTCAGGCTGAAAACATGAC+TGG 0.593607 3_2:+60249524 Msa0414630:CDS
AAAGCAGCTATATTCGTTGG+TGG 0.594023 3_2:+60250655 Msa0414630:CDS
CAGTACAAGTACAAAGAATG+TGG 0.595619 3_2:+60250452 Msa0414630:CDS
AAAGGCATATAGGTACTGGT+TGG 0.596578 3_2:-60249329 None:intergenic
GAAATGAAGCCTCAAAAGGA+AGG 0.600514 3_2:+60250243 Msa0414630:CDS
GAAGACATTGTTTCAAACGA+TGG 0.612365 3_2:+60248345 Msa0414630:CDS
CGGGACTACTTACAGGCGAA+TGG 0.614446 3_2:+60250296 Msa0414630:CDS
CAAACACTTCAAGCAGTTGG+AGG 0.616012 3_2:+60250386 Msa0414630:CDS
ATTGCTGACTACTATAGAGA+AGG 0.617607 3_2:+60250333 Msa0414630:CDS
GCTGACTACTATAGAGAAGG+TGG 0.619318 3_2:+60250336 Msa0414630:CDS
AGGAAACATATGAAGTTCAG+AGG 0.620711 3_2:-60250782 None:intergenic
CCATGCCCAGTAACAATCAA+CGG 0.629569 3_2:-60249095 None:intergenic
ATTCTCAAAAGAGGTTGCAT+CGG 0.629928 3_2:-60249372 None:intergenic
CGTACTCGATGGCTATATGG+CGG 0.638966 3_2:+60250276 Msa0414630:CDS
TGGAAAGTTGATAAAGAACG+AGG 0.646078 3_2:+60248381 Msa0414630:CDS
GGATGTCATTTGATCCAACA+AGG 0.647593 3_2:+60250043 Msa0414630:CDS
ACTTGATATATTGAAATCTG+AGG 0.651550 3_2:+60248590 Msa0414630:CDS
GAACCAAAGGTGATGCAGAG+TGG 0.654270 3_2:-60249188 None:intergenic
AGATCGTTAGTTCAAATCCA+CGG 0.689699 3_2:+60249069 Msa0414630:intron
AATCTCCTATCTCCAACAAG+GGG 0.702048 3_2:-60249212 None:intergenic
TTGTGCCAAGAAAATATCTG+CGG 0.707071 3_2:-60250634 None:intergenic
TATGGAATTGAACAACATCG+TGG 0.722755 3_2:-60250179 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGACTATGTTAATAATAAA+AGG + chr3_2:60250361-60250380 Msa0414630:CDS 15.0%
!! TTGCATTTATTTCATTATTC+TGG + chr3_2:60248632-60248651 Msa0414630:intron 20.0%
!!! CATTTTCTCAAATTATTATC+CGG + chr3_2:60248893-60248912 Msa0414630:intron 20.0%
!! ATATCACTATTAAATAGCAT+TGG + chr3_2:60249149-60249168 Msa0414630:CDS 20.0%
! TTTGACCATTATAGCATTTA+AGG + chr3_2:60248410-60248429 Msa0414630:CDS 25.0%
! CAGATTTCAATATATCAAGT+TGG - chr3_2:60248590-60248609 None:intergenic 25.0%
! ACTTGATATATTGAAATCTG+AGG + chr3_2:60248590-60248609 Msa0414630:CDS 25.0%
! GCATTAAGATTGTTACATAA+GGG - chr3_2:60249049-60249068 None:intergenic 25.0%
! TGCATTAAGATTGTTACATA+AGG - chr3_2:60249050-60249069 None:intergenic 25.0%
!!! TTGATTTTTATCATGGACTA+AGG - chr3_2:60249432-60249451 None:intergenic 25.0%
! TTAGTCCATGATAAAAATCA+AGG + chr3_2:60249431-60249450 Msa0414630:CDS 25.0%
! TTTCCATAATCAATAGTTTC+AGG - chr3_2:60249458-60249477 None:intergenic 25.0%
!! TTGCATAAATTTTTCCATTC+AGG + chr3_2:60249508-60249527 Msa0414630:CDS 25.0%
! TTTGGAAATAATCATCAGTT+TGG - chr3_2:60249780-60249799 None:intergenic 25.0%
! GCTAAATGTTATAATTACAG+TGG + chr3_2:60249851-60249870 Msa0414630:intron 25.0%
!!! TTACAGTGGATTAAATAAAG+AGG + chr3_2:60249865-60249884 Msa0414630:intron 25.0%
!! CATGAAAAATTAGAGTTTTC+AGG - chr3_2:60249917-60249936 None:intergenic 25.0%
! ATTTCATTCAATTTCCTTGT+TGG - chr3_2:60250060-60250079 None:intergenic 25.0%
! AAGGAAATTGAATGAAATGA+AGG + chr3_2:60250062-60250081 Msa0414630:CDS 25.0%
! GAAGAAATTGTTTGAATTTG+AGG + chr3_2:60250575-60250594 Msa0414630:CDS 25.0%
!! AATTTGAGGTGTATGTTTAT+GGG + chr3_2:60250589-60250608 Msa0414630:CDS 25.0%
!! AAGGAGTATAAAGAGATTAA+GGG + chr3_2:60250678-60250697 Msa0414630:CDS 25.0%
! TAATCTATCAAACCAAAGAA+AGG - chr3_2:60250805-60250824 None:intergenic 25.0%
!!! TTTCTTTGGTTTGATAGATT+AGG + chr3_2:60250804-60250823 Msa0414630:three_prime_UTR 25.0%
! GATAGATTAGGATGATTATA+TGG + chr3_2:60250816-60250835 Msa0414630:three_prime_UTR 25.0%
CTAGTATACTTTGTAAGTCA+TGG + chr3_2:60248295-60248314 Msa0414630:CDS 30.0%
TCAGAAGAAGAAATCAAATC+AGG - chr3_2:60248462-60248481 None:intergenic 30.0%
GATTTCTTCTTCTGAACTTA+AGG + chr3_2:60248467-60248486 Msa0414630:CDS 30.0%
TTGCATTGTAACACTGAATT+AGG + chr3_2:60248812-60248831 Msa0414630:intron 30.0%
! AAATGTTCCAAAAGGCATAT+AGG - chr3_2:60249342-60249361 None:intergenic 30.0%
CACATGATAAATGTTCCAAA+AGG - chr3_2:60249350-60249369 None:intergenic 30.0%
! ATCTATCTTGGCAATTTTTG+TGG + chr3_2:60249400-60249419 Msa0414630:CDS 30.0%
CCTATTGAGTATGATTCAAT+CGG - chr3_2:60249647-60249666 None:intergenic 30.0%
! GAATGAAATGAAGGTACATA+TGG + chr3_2:60250071-60250090 Msa0414630:CDS 30.0%
!! CTTTTTGTACCATTCAAGTT+GGG - chr3_2:60250097-60250116 None:intergenic 30.0%
! TGACTATAGATTCTTGCTTT+TGG + chr3_2:60250484-60250503 Msa0414630:CDS 30.0%
!! GACTATAGATTCTTGCTTTT+GGG + chr3_2:60250485-60250504 Msa0414630:CDS 30.0%
! GAATTTGAGGTGTATGTTTA+TGG + chr3_2:60250588-60250607 Msa0414630:CDS 30.0%
! GAAGGAGTATAAAGAGATTA+AGG + chr3_2:60250677-60250696 Msa0414630:CDS 30.0%
! AAAATTGAGAAGTATGCTAC+AGG + chr3_2:60250747-60250766 Msa0414630:CDS 30.0%
CTTCATATGTTTCCTTTCTT+TGG + chr3_2:60250790-60250809 Msa0414630:three_prime_UTR 30.0%
!!! CTATTTCTGTTTTAGAACTC+TGG + chr3_2:60250914-60250933 Msa0414630:three_prime_UTR 30.0%
GAAGACATTGTTTCAAACGA+TGG + chr3_2:60248345-60248364 Msa0414630:CDS 35.0%
ATTGTTTCAAACGATGGTGT+TGG + chr3_2:60248351-60248370 Msa0414630:CDS 35.0%
!! TGGAAAGTTGATAAAGAACG+AGG + chr3_2:60248381-60248400 Msa0414630:CDS 35.0%
TGCAGCCTTAAATGCTATAA+TGG - chr3_2:60248418-60248437 None:intergenic 35.0%
TTCTTCTTCTGAACTTAAGG+AGG + chr3_2:60248470-60248489 Msa0414630:CDS 35.0%
AACACAGAAAGTCAAAGTCA+TGG - chr3_2:60248503-60248522 None:intergenic 35.0%
!! GTTTTGTTAAGAGAGAAGGT+TGG - chr3_2:60248537-60248556 None:intergenic 35.0%
!!! TGCAGTTTTGTTAAGAGAGA+AGG - chr3_2:60248541-60248560 None:intergenic 35.0%
ACAAATTTCCTGTAGTTGTG+TGG + chr3_2:60248658-60248677 Msa0414630:intron 35.0%
TGTGTATCTGCTTCATTCTT+TGG + chr3_2:60248731-60248750 Msa0414630:intron 35.0%
GTATCTAGACTCATCTTTCT+AGG + chr3_2:60249001-60249020 Msa0414630:intron 35.0%
CATAAGGGATTGTAGTGAAA+AGG - chr3_2:60249034-60249053 None:intergenic 35.0%
AGATCGTTAGTTCAAATCCA+CGG + chr3_2:60249069-60249088 Msa0414630:intron 35.0%
ATTCTCAAAAGAGGTTGCAT+CGG - chr3_2:60249375-60249394 None:intergenic 35.0%
AGATTCTGCATTCTCAAAAG+AGG - chr3_2:60249384-60249403 None:intergenic 35.0%
TGAGAATGCAGAATCTATCT+TGG + chr3_2:60249388-60249407 Msa0414630:CDS 35.0%
! CAGGTCCTTGATTTTTATCA+TGG - chr3_2:60249439-60249458 None:intergenic 35.0%
! GGACCTGAAACTATTGATTA+TGG + chr3_2:60249452-60249471 Msa0414630:CDS 35.0%
TATTCACTTACCTGCATTTG+TGG - chr3_2:60249605-60249624 None:intergenic 35.0%
CCGATTGAATCATACTCAAT+AGG + chr3_2:60249644-60249663 Msa0414630:intron 35.0%
TGAATCATACTCAATAGGTG+TGG + chr3_2:60249649-60249668 Msa0414630:intron 35.0%
TGAACTTGTGAGAGTGATTA+AGG + chr3_2:60249692-60249711 Msa0414630:intron 35.0%
TCACAAAGTCTAAGCTTGTA+GGG + chr3_2:60249806-60249825 Msa0414630:intron 35.0%
TAAGCTTGTAGGGATAATCT+GGG + chr3_2:60249816-60249835 Msa0414630:intron 35.0%
CCAAGTCAATTTCAGAAATG+AGG - chr3_2:60249941-60249960 None:intergenic 35.0%
!! CCTCATTTCTGAAATTGACT+TGG + chr3_2:60249938-60249957 Msa0414630:intron 35.0%
GAACAGTGAAATCAATACTC+TGG + chr3_2:60249975-60249994 Msa0414630:CDS 35.0%
AAAGATTCATCCTACAGAAG+AGG + chr3_2:60250022-60250041 Msa0414630:CDS 35.0%
! CCTTTTTGTACCATTCAAGT+TGG - chr3_2:60250098-60250117 None:intergenic 35.0%
CCAACTTGAATGGTACAAAA+AGG + chr3_2:60250095-60250114 Msa0414630:CDS 35.0%
AATCTAAGAATCAGCAAGCA+GGG + chr3_2:60250118-60250137 Msa0414630:CDS 35.0%
TATGGAATTGAACAACATCG+TGG - chr3_2:60250182-60250201 None:intergenic 35.0%
AGTTGAAATGAAGCCTCAAA+AGG + chr3_2:60250239-60250258 Msa0414630:CDS 35.0%
TAGTAGTCAGCAATAGCTAA+AGG - chr3_2:60250327-60250346 None:intergenic 35.0%
! ATTGCTGACTACTATAGAGA+AGG + chr3_2:60250333-60250352 Msa0414630:CDS 35.0%
! GTTGATGGAAGAGTCAAAAA+AGG + chr3_2:60250413-60250432 Msa0414630:CDS 35.0%
TGTACTGCTTATATCACTTG+TGG - chr3_2:60250439-60250458 None:intergenic 35.0%
CAGTACAAGTACAAAGAATG+TGG + chr3_2:60250452-60250471 Msa0414630:CDS 35.0%
CCTTCAACTCTTTGCATAAA+AGG - chr3_2:60250530-60250549 None:intergenic 35.0%
! CCTTTTATGCAAAGAGTTGA+AGG + chr3_2:60250527-60250546 Msa0414630:CDS 35.0%
TTGTGCCAAGAAAATATCTG+CGG - chr3_2:60250637-60250656 None:intergenic 35.0%
AGGAAACATATGAAGTTCAG+AGG - chr3_2:60250785-60250804 None:intergenic 35.0%
GAGCAATTCATAGATTGAAG+TGG + chr3_2:60250845-60250864 Msa0414630:three_prime_UTR 35.0%
!! ATGAAGCACTGACACAAAGA+CGG - chr3_2:60248922-60248941 None:intergenic 40.0%
TCTTTGTGTCAGTGCTTCAT+AGG + chr3_2:60248922-60248941 Msa0414630:intron 40.0%
AATCTCCTATCTCCAACAAG+GGG - chr3_2:60249215-60249234 None:intergenic 40.0%
CAATCTCCTATCTCCAACAA+GGG - chr3_2:60249216-60249235 None:intergenic 40.0%
CCAAATAGAACTACGTGAGA+TGG - chr3_2:60249246-60249265 None:intergenic 40.0%
! CCATCTCACGTAGTTCTATT+TGG + chr3_2:60249243-60249262 Msa0414630:CDS 40.0%
!! AAAGGCATATAGGTACTGGT+TGG - chr3_2:60249332-60249351 None:intergenic 40.0%
! ACCAGTACCTATATGCCTTT+TGG + chr3_2:60249332-60249351 Msa0414630:CDS 40.0%
! TCCAAAAGGCATATAGGTAC+TGG - chr3_2:60249336-60249355 None:intergenic 40.0%
! CTTGGCAATTTTTGTGGCAT+TGG + chr3_2:60249406-60249425 Msa0414630:CDS 40.0%
! AGTCATGTTTTCAGCCTGAA+TGG - chr3_2:60249525-60249544 None:intergenic 40.0%
ATTCAGGCTGAAAACATGAC+TGG + chr3_2:60249524-60249543 Msa0414630:CDS 40.0%
!! AACTGGCATTAGTTTGCAGT+TGG + chr3_2:60249562-60249581 Msa0414630:CDS 40.0%
AGAATTGACACCACAAATGC+AGG + chr3_2:60249592-60249611 Msa0414630:CDS 40.0%
CTCAAAAGAGGGTTGTTCTT+TGG - chr3_2:60249726-60249745 None:intergenic 40.0%
! AAGAACAACCCTCTTTTGAG+AGG + chr3_2:60249726-60249745 Msa0414630:intron 40.0%
!! AGAACAACCCTCTTTTGAGA+GGG + chr3_2:60249727-60249746 Msa0414630:intron 40.0%
TCTTCATCCCTCTCAAAAGA+GGG - chr3_2:60249737-60249756 None:intergenic 40.0%
! TAGACTTTGTGAGTTGGCTT+TGG - chr3_2:60249798-60249817 None:intergenic 40.0%
CAAGCTTAGACTTTGTGAGT+TGG - chr3_2:60249804-60249823 None:intergenic 40.0%
CTCACAAAGTCTAAGCTTGT+AGG + chr3_2:60249805-60249824 Msa0414630:intron 40.0%
CTAAGCTTGTAGGGATAATC+TGG + chr3_2:60249815-60249834 Msa0414630:intron 40.0%
!! TCTGAAATTGACTTGGCAAC+AGG + chr3_2:60249945-60249964 Msa0414630:intron 40.0%
CAAATGACATCCTCTTCTGT+AGG - chr3_2:60250035-60250054 None:intergenic 40.0%
! GGATGTCATTTGATCCAACA+AGG + chr3_2:60250043-60250062 Msa0414630:CDS 40.0%
TACATATGGCCCAACTTGAA+TGG + chr3_2:60250085-60250104 Msa0414630:CDS 40.0%
GAATCTAAGAATCAGCAAGC+AGG + chr3_2:60250117-60250136 Msa0414630:CDS 40.0%
AGTAACTGGTGAGCTTCTTA+TGG - chr3_2:60250200-60250219 None:intergenic 40.0%
ATAAGAAGCTCACCAGTTAC+TGG + chr3_2:60250199-60250218 Msa0414630:CDS 40.0%
TAAGAAGCTCACCAGTTACT+GGG + chr3_2:60250200-60250219 Msa0414630:CDS 40.0%
GAAATGAAGCCTCAAAAGGA+AGG + chr3_2:60250243-60250262 Msa0414630:CDS 40.0%
!!! AAAGAAGCACCTTCCTTTTG+AGG - chr3_2:60250255-60250274 None:intergenic 40.0%
TTTCGTACTCGATGGCTATA+TGG + chr3_2:60250273-60250292 Msa0414630:CDS 40.0%
! GTATGTTTATGGGTTGCTCA+AGG + chr3_2:60250599-60250618 Msa0414630:CDS 40.0%
! AGTGTCCGCAGATATTTTCT+TGG + chr3_2:60250629-60250648 Msa0414630:CDS 40.0%
CACAAAGCAGCTATATTCGT+TGG + chr3_2:60250652-60250671 Msa0414630:CDS 40.0%
AAAGCAGCTATATTCGTTGG+TGG + chr3_2:60250655-60250674 Msa0414630:CDS 40.0%
! ACGATGGTGTTGGACTTTCT+TGG + chr3_2:60248361-60248380 Msa0414630:CDS 45.0%
TTCCTGTAGTTGTGTGGCTA+TGG + chr3_2:60248664-60248683 Msa0414630:intron 45.0%
CCATGCCCAGTAACAATCAA+CGG - chr3_2:60249098-60249117 None:intergenic 45.0%
ATTGTTACTGGGCATGGTCT+TGG + chr3_2:60249101-60249120 Msa0414630:CDS 45.0%
GCAATCTCCTATCTCCAACA+AGG - chr3_2:60249217-60249236 None:intergenic 45.0%
! TAATGAAGCGCCTTGGAAGT+GGG - chr3_2:60249304-60249323 None:intergenic 45.0%
GGTCAAGATTCACTAGCAAC+TGG + chr3_2:60249545-60249564 Msa0414630:CDS 45.0%
CTCTTCATCCCTCTCAAAAG+AGG - chr3_2:60249738-60249757 None:intergenic 45.0%
!! ATTGACTTGGCAACAGGAAC+AGG + chr3_2:60249951-60249970 Msa0414630:intron 45.0%
AACCATCTTCTCCCAGTAAC+TGG - chr3_2:60250214-60250233 None:intergenic 45.0%
! GTGCTTCTTTTCGTACTCGA+TGG + chr3_2:60250265-60250284 Msa0414630:CDS 45.0%
! TATATGGCGGGACTACTTAC+AGG + chr3_2:60250289-60250308 Msa0414630:CDS 45.0%
GCTGACTACTATAGAGAAGG+TGG + chr3_2:60250336-60250355 Msa0414630:CDS 45.0%
GTCCAAACACTTCAAGCAGT+TGG + chr3_2:60250383-60250402 Msa0414630:CDS 45.0%
! CTCCAACTGCTTGAAGTGTT+TGG - chr3_2:60250388-60250407 None:intergenic 45.0%
CAAACACTTCAAGCAGTTGG+AGG + chr3_2:60250386-60250405 Msa0414630:CDS 45.0%
! CTTTTGGGCACATGTTGAAG+AGG + chr3_2:60250500-60250519 Msa0414630:CDS 45.0%
CAGTGTCTCTTCTCTCTCAT+TGG - chr3_2:60250556-60250575 None:intergenic 45.0%
! CAGCTATATTCGTTGGTGGA+AGG + chr3_2:60250659-60250678 Msa0414630:CDS 45.0%
AGCCATAGCCACACAACTAC+AGG - chr3_2:60248669-60248688 None:intergenic 50.0%
ACACTGAATTAGGTGTGCCC+CGG + chr3_2:60248822-60248841 Msa0414630:intron 50.0%
ACTGACACAAAGACGGACAC+CGG - chr3_2:60248915-60248934 None:intergenic 50.0%
! GGACGCCGTTGATTGTTACT+GGG + chr3_2:60249090-60249109 Msa0414630:CDS 50.0%
! CCGTTGATTGTTACTGGGCA+TGG + chr3_2:60249095-60249114 Msa0414630:CDS 50.0%
GAACCAAAGGTGATGCAGAG+TGG - chr3_2:60249191-60249210 None:intergenic 50.0%
! ACCTTTGGTTCACCCCTTGT+TGG + chr3_2:60249200-60249219 Msa0414630:CDS 50.0%
TCCAACAAGGGGTGAACCAA+AGG - chr3_2:60249204-60249223 None:intergenic 50.0%
GTTCACCCCTTGTTGGAGAT+AGG + chr3_2:60249207-60249226 Msa0414630:CDS 50.0%
! GTAATGAAGCGCCTTGGAAG+TGG - chr3_2:60249305-60249324 None:intergenic 50.0%
! GGACTTGTAATGAAGCGCCT+TGG - chr3_2:60249311-60249330 None:intergenic 50.0%
CACCAGTTACTGGGAGAAGA+TGG + chr3_2:60250209-60250228 Msa0414630:CDS 50.0%
CGTACTCGATGGCTATATGG+CGG + chr3_2:60250276-60250295 Msa0414630:CDS 50.0%
GTACTCGATGGCTATATGGC+GGG + chr3_2:60250277-60250296 Msa0414630:CDS 50.0%
ACTTCAAGCAGTTGGAGGAG+TGG + chr3_2:60250391-60250410 Msa0414630:CDS 50.0%
TCGTGTCAGTGTCTGACACC+GGG - chr3_2:60248843-60248862 None:intergenic 55.0%
GTCGTGTCAGTGTCTGACAC+CGG - chr3_2:60248844-60248863 None:intergenic 55.0%
GTAACAATCAACGGCGTCCG+TGG - chr3_2:60249089-60249108 None:intergenic 55.0%
! CGGACGCCGTTGATTGTTAC+TGG + chr3_2:60249089-60249108 Msa0414630:CDS 55.0%
! GTTACTGGGCATGGTCTTGG+AGG + chr3_2:60249104-60249123 Msa0414630:CDS 55.0%
CGTCCACTCTGCATCACCTT+TGG + chr3_2:60249185-60249204 Msa0414630:CDS 55.0%
CACACAGAGACCCACTTCCA+AGG + chr3_2:60249291-60249310 Msa0414630:CDS 55.0%
CGGGACTACTTACAGGCGAA+TGG + chr3_2:60250296-60250315 Msa0414630:CDS 55.0%
GCAGTTGGAGGAGTGGTTGA+TGG + chr3_2:60250398-60250417 Msa0414630:CDS 55.0%
CGTGTCAGTGTCTGACACCG+GGG - chr3_2:60248842-60248861 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 60248282 60250957 60248282 ID=Msa0414630;Name=Msa0414630
chr3_2 mRNA 60248282 60250957 60248282 ID=Msa0414630-mRNA-1;Parent=Msa0414630;Name=Msa0414630-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|1|0.66|1|1|1|3|169|564
chr3_2 exon 60248282 60248611 60248282 ID=Msa0414630-mRNA-1:exon:12544;Parent=Msa0414630-mRNA-1
chr3_2 exon 60249071 60249613 60249071 ID=Msa0414630-mRNA-1:exon:12545;Parent=Msa0414630-mRNA-1
chr3_2 exon 60249967 60250957 60249967 ID=Msa0414630-mRNA-1:exon:12546;Parent=Msa0414630-mRNA-1
chr3_2 CDS 60248282 60248611 60248282 ID=Msa0414630-mRNA-1:cds;Parent=Msa0414630-mRNA-1
chr3_2 CDS 60249071 60249613 60249071 ID=Msa0414630-mRNA-1:cds;Parent=Msa0414630-mRNA-1
chr3_2 CDS 60249967 60250788 60249967 ID=Msa0414630-mRNA-1:cds;Parent=Msa0414630-mRNA-1
chr3_2 three_prime_UTR 60250789 60250957 60250789 ID=Msa0414630-mRNA-1:three_prime_utr;Parent=Msa0414630-mRNA-1
Gene Sequence

>Msa0414630

ATGGTGGCAAGTTCTAGTATACTTTGTAAGTCATGGAGTACTATCTCATCTGAATCTGATAATGAAGACATTGTTTCAAACGATGGTGTTGGACTTTCTTGGAAAGTTGATAAAGAACGAGGTTCAGATTTGACCATTATAGCATTTAAGGCTGCATTAGATTCTTCTAATGTTCAACCTGATTTGATTTCTTCTTCTGAACTTAAGGAGGACAATTTCCATGACTTTGACTTTCTGTGTTCTACAAGTATTCCAACCTTCTCTCTTAACAAAACTGCACTTTCACTCTTTCGTGACAATCATCACCAACTTGATATATTGAAATCTGAGATCGTTAGTTCAAATCCACGGACGCCGTTGATTGTTACTGGGCATGGTCTTGGAGGATCAATTGCTTCTCTCTTTATAATATCACTATTAAATAGCATTGGTTCAACAAAGAATCGTCCACTCTGCATCACCTTTGGTTCACCCCTTGTTGGAGATAGGAGATTGCAACAAGCCATCTCACGTAGTTCTATTTGGAATTCTTGCTTCATTCACGTTGTGTCACACAGAGACCCACTTCCAAGGCGCTTCATTACAAGTCCAACCAGTACCTATATGCCTTTTGGAACATTTATCATGTGTTCCGATGCAACCTCTTTTGAGAATGCAGAATCTATCTTGGCAATTTTTGTGGCATTGGCCTTAGTCCATGATAAAAATCAAGGACCTGAAACTATTGATTATGGAAATGTAGTAAAAAATATCTATCTTAAAGCAACTTGCATAAATTTTTCCATTCAGGCTGAAAACATGACTGGTCAAGATTCACTAGCAACTGGCATTAGTTTGCAGTTGGCAGCTCTAGAATTGACACCACAAATGCAGGAACAGGAGAACAGTGAAATCAATACTCTGGACACAAAGTTAAAAGAACTAGAAGAAAGATTCATCCTACAGAAGAGGATGTCATTTGATCCAACAAGGAAATTGAATGAAATGAAGGTACATATGGCCCAACTTGAATGGTACAAAAAGGAATCTAAGAATCAGCAAGCAGGGTACTATGACAGCTACAAAAACTTGAACTCAACATTTGACCACGATGTTGTTCAATTCCATAAGAAGCTCACCAGTTACTGGGAGAAGATGGTTGAAGAAGTTGAAATGAAGCCTCAAAAGGAAGGTGCTTCTTTTCGTACTCGATGGCTATATGGCGGGACTACTTACAGGCGAATGGTTGAACCTTTAGCTATTGCTGACTACTATAGAGAAGGTGGCAAAGACTATGTTAATAATAAAAGGTCCAAACACTTCAAGCAGTTGGAGGAGTGGTTGATGGAAGAGTCAAAAAAGGCCACAAGTGATATAAGCAGTACAAGTACAAAGAATGTGGAAGCTATTTTGACTATAGATTCTTGCTTTTGGGCACATGTTGAAGAGGCTATCCTTTTATGCAAAGAGTTGAAGGTTGCCAATGAGAGAGAAGAGACACTGAAGAAATTGTTTGAATTTGAGGTGTATGTTTATGGGTTGCTCAAGGATTATGCAGTGTCCGCAGATATTTTCTTGGCACAAAGCAGCTATATTCGTTGGTGGAAGGAGTATAAAGAGATTAAGGGATCATTATACACTTCTGCACTCGTGAACTTCATGAACGATGATACAAAAATTGAGAAGTATGCTACAGGAGCTTATGATTTCCTCTGA
Protein sequence

>Msa0414630

MVASSSILCKSWSTISSESDNEDIVSNDGVGLSWKVDKERGSDLTIIAFKAALDSSNVQPDLISSSELKEDNFHDFDFLCSTSIPTFSLNKTALSLFRDNHHQLDILKSEIVSSNPRTPLIVTGHGLGGSIASLFIISLLNSIGSTKNRPLCITFGSPLVGDRRLQQAISRSSIWNSCFIHVVSHRDPLPRRFITSPTSTYMPFGTFIMCSDATSFENAESILAIFVALALVHDKNQGPETIDYGNVVKNIYLKATCINFSIQAENMTGQDSLATGISLQLAALELTPQMQEQENSEINTLDTKLKELEERFILQKRMSFDPTRKLNEMKVHMAQLEWYKKESKNQQAGYYDSYKNLNSTFDHDVVQFHKKLTSYWEKMVEEVEMKPQKEGASFRTRWLYGGTTYRRMVEPLAIADYYREGGKDYVNNKRSKHFKQLEEWLMEESKKATSDISSTSTKNVEAILTIDSCFWAHVEEAILLCKELKVANEREETLKKLFEFEVYVYGLLKDYAVSADIFLAQSSYIRWWKEYKEIKGSLYTSALVNFMNDDTKIEKYATGAYDFL*