AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0418310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418310 MtrunA17_Chr3g0116021 92.308 182 14 0 1 182 1 182 6.35e-122 347
Msa0418310 MtrunA17_Chr5g0426491 61.176 170 66 0 1 170 1 170 9.72e-74 224
Msa0418310 MtrunA17_Chr5g0448101 58.036 112 47 0 71 182 1 112 2.76e-39 132
Msa0418310 MtrunA17_Chr1g0168481 32.919 161 100 3 9 161 22 182 2.61e-15 72.0
Msa0418310 MtrunA17_Chr1g0168491 29.814 161 105 3 9 161 22 182 3.14e-11 60.5
Msa0418310 MtrunA17_Chr3g0111381 27.632 152 107 3 9 158 21 171 9.63e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418310 AT4G08980.5 57.923 183 76 1 1 182 1 183 3.49e-72 221
Msa0418310 AT4G08980.4 57.923 183 76 1 1 182 1 183 3.49e-72 221
Msa0418310 AT4G08980.1 57.923 183 76 1 1 182 1 183 3.49e-72 221
Msa0418310 AT4G08980.2 57.923 183 76 1 1 182 1 183 3.49e-72 221
Msa0418310 AT4G08980.3 57.923 183 76 1 1 182 1 183 3.49e-72 221
Msa0418310 AT4G05460.1 27.976 168 115 4 3 167 12 176 6.17e-12 63.2
Msa0418310 AT5G57900.1 30.114 176 109 5 1 165 1 173 1.31e-11 62.0

Find 64 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGAAAGCTGTGTGTTTC+TGG 0.141960 3_2:+66012876 Msa0418310:CDS
CTTTACTCTTGTTCTTGTTT+TGG 0.240556 3_2:+66012088 Msa0418310:five_prime_UTR
TCATGGTGTAAAGCAGTTTC+TGG 0.263962 3_2:+66012747 Msa0418310:CDS
TTGTTCGAGGAGGTGAATTA+TGG 0.266030 3_2:-66012269 None:intergenic
TTCAGCAATGAAAGTGAAAA+TGG 0.303416 3_2:-66012913 None:intergenic
GAATTGATTCCTGATGCATT+AGG 0.312469 3_2:+66012663 Msa0418310:CDS
AGGTATAATTCCTAGCTAAA+TGG 0.323030 3_2:-66013659 None:intergenic
GAAGAAGGTGAAGATGAAAA+AGG 0.330310 3_2:-66012197 None:intergenic
GTGAGCTATTGTATCAAAAT+TGG 0.365727 3_2:+66013543 Msa0418310:CDS
TCTATCTCTTGCCAACAATT+AGG 0.369302 3_2:-66012771 None:intergenic
AATTGATTCCTGATGCATTA+GGG 0.374472 3_2:+66012664 Msa0418310:CDS
TTGTTATAGATTGGATTTGA+TGG 0.392983 3_2:+66012611 Msa0418310:intron
GACCATGCTTAGCTAGGAAA+TGG 0.407990 3_2:+66013754 Msa0418310:three_prime_UTR
CACCATGATTTACATACCCT+TGG 0.409233 3_2:-66012732 None:intergenic
TCTTTCCCCGTTGCATGTGC+CGG 0.418425 3_2:+66012228 Msa0418310:five_prime_UTR
AAGGAGCAATATGAGCGATT+TGG 0.420691 3_2:+66013473 Msa0418310:CDS
TTGGATTTGATGGGAGAAGC+AGG 0.430121 3_2:+66012621 Msa0418310:exon
TGTATTGAAGCAAAAGTGTT+AGG 0.433058 3_2:+66012119 Msa0418310:five_prime_UTR
TTGGTTGTGGAACAGATTGC+TGG 0.436111 3_2:+66013492 Msa0418310:CDS
AGCAGGTGAATTACGAAGTT+GGG 0.441077 3_2:+66012638 Msa0418310:CDS
AGCATCTGATCAAGTTTGTC+AGG 0.441867 3_2:-66012822 None:intergenic
TTTCAAGTGCTGGTTCCCTT+CGG 0.448249 3_2:+66013436 Msa0418310:intron
AAGATCACCCCTAATGCATC+AGG 0.454304 3_2:-66012672 None:intergenic
CAGTTTCTGGACCTAATTGT+TGG 0.459386 3_2:+66012760 Msa0418310:CDS
CATACCGAGGAAAGTTTCCT+AGG 0.474553 3_2:+66013712 Msa0418310:three_prime_UTR
CAATATGAGCGATTTGGTTG+TGG 0.479679 3_2:+66013479 Msa0418310:CDS
CCGTTTGACCATGCTTAGCT+AGG 0.484522 3_2:+66013748 Msa0418310:three_prime_UTR
AGAGAGTGACAGTGATTCCA+AGG 0.502069 3_2:+66012715 Msa0418310:CDS
TGTTATAGATTGGATTTGAT+GGG 0.503271 3_2:+66012612 Msa0418310:intron
TACAAGAGAGTGTTTGTTCG+AGG 0.503287 3_2:-66012282 None:intergenic
AAGCAGGTGAATTACGAAGT+TGG 0.504117 3_2:+66012637 Msa0418310:CDS
GCGTGTGCAATTGAGATGAT+AGG 0.513987 3_2:+66013567 Msa0418310:CDS
GGAATTATACCTAGGAATCC+AGG 0.516374 3_2:+66013670 Msa0418310:exon
TCTGATCAAGTTTGTCAGGT+TGG 0.524395 3_2:-66012818 None:intergenic
AAGAAATCATCCATTTAGCT+AGG 0.529152 3_2:+66013649 Msa0418310:CDS
AAATCGCTCATATTGCTCCT+TGG 0.539756 3_2:-66013471 None:intergenic
GGAAAGAAGAGATAAGAAGA+AGG 0.540882 3_2:-66012212 None:intergenic
CCTAGCTAAGCATGGTCAAA+CGG 0.547872 3_2:-66013748 None:intergenic
AGCGAGGAACCTGGATTCCT+AGG 0.549205 3_2:-66013679 None:intergenic
TTCCAAGGGTATGTAAATCA+TGG 0.555605 3_2:+66012730 Msa0418310:CDS
TTGGTAGTCTCAAACTCCGA+AGG 0.580644 3_2:-66013452 None:intergenic
ATTATCTGTAGCGAGGAACC+TGG 0.581119 3_2:-66013688 None:intergenic
ATGCTTATCAGTAAAAGTTG+TGG 0.582915 3_2:+66012849 Msa0418310:CDS
AAGAGATAGATATTGAAGAA+TGG 0.583086 3_2:+66012784 Msa0418310:CDS
TTTAGCTAGGAATTATACCT+AGG 0.584695 3_2:+66013662 Msa0418310:CDS
GTGTAGGAATATGCATCCAT+TGG 0.590372 3_2:+66013617 Msa0418310:CDS
ATTGAAGCAAAAGTGTTAGG+TGG 0.593296 3_2:+66012122 Msa0418310:five_prime_UTR
CTTACCTAGGAAACTTTCCT+CGG 0.595373 3_2:-66013716 None:intergenic
CTCCATTTCCTAGCTAAGCA+TGG 0.596689 3_2:-66013756 None:intergenic
AACTGCTAGAAGAGTTGTGT+AGG 0.601293 3_2:+66013601 Msa0418310:CDS
TTACCTAGGAAATATTTCCT+CGG 0.607165 3_2:-66013786 None:intergenic
ATTTCTTGTAGTGTATCCAA+TGG 0.608133 3_2:-66013633 None:intergenic
ACGGTCAAATATGCTTACCT+AGG 0.608359 3_2:-66013729 None:intergenic
AAGAGAGTGTTTGTTCGAGG+AGG 0.614233 3_2:-66012279 None:intergenic
TGGTAGTCTCAAACTCCGAA+GGG 0.622847 3_2:-66013451 None:intergenic
CATGCTTAGCTAGGAAATGG+AGG 0.628361 3_2:+66013757 Msa0418310:three_prime_UTR
GAGAGTGACAGTGATTCCAA+GGG 0.645348 3_2:+66012716 Msa0418310:CDS
GCTTAGCTAGGAAATGGAGG+TGG 0.664209 3_2:+66013760 Msa0418310:three_prime_UTR
GGAAATGGAGGTGGTTACCG+AGG 0.664257 3_2:+66013769 Msa0418310:three_prime_UTR
ATTGATTCCTGATGCATTAG+GGG 0.669573 3_2:+66012665 Msa0418310:CDS
TTCGGAGTTTGAGACTACCA+AGG 0.691268 3_2:+66013454 Msa0418310:CDS
GGTATGCATTATCTGTAGCG+AGG 0.697058 3_2:-66013695 None:intergenic
GCTACAGATAATGCATACCG+AGG 0.713831 3_2:+66013699 Msa0418310:three_prime_UTR
AAAATCCGGCACATGCAACG+GGG 0.735285 3_2:-66012233 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACAAAAATCTTTCT+GGG + chr3_2:66012398-66012417 Msa0418310:intron 15.0%
!! AACTTTATGTTCATTTAATA+TGG + chr3_2:66012517-66012536 Msa0418310:intron 15.0%
!!! TTTTACTTTTATATAGTTTG+TGG - chr3_2:66012577-66012596 None:intergenic 15.0%
!! AGATGAAAAAACATAAAATT+TGG + chr3_2:66013298-66013317 Msa0418310:intron 15.0%
!! AAAAAAACAAAAATCTTTCT+GGG + chr3_2:66012398-66012417 Msa0418310:intron 15.0%
!! AACTTTATGTTCATTTAATA+TGG + chr3_2:66012517-66012536 Msa0418310:intron 15.0%
!!! TTTTACTTTTATATAGTTTG+TGG - chr3_2:66012577-66012596 None:intergenic 15.0%
!! AGATGAAAAAACATAAAATT+TGG + chr3_2:66013298-66013317 Msa0418310:intron 15.0%
!! CAAAAAAACAAAAATCTTTC+TGG + chr3_2:66012397-66012416 Msa0418310:intron 20.0%
!! TATCAGAAAAATAACACATA+AGG - chr3_2:66012472-66012491 None:intergenic 20.0%
!! TAAAGTTGAGAGAATAAAAA+TGG - chr3_2:66012504-66012523 None:intergenic 20.0%
!!! GATTTTTCTTTGTTATAGAT+TGG + chr3_2:66012602-66012621 Msa0418310:intron 20.0%
!! TTGTTAAAATGTTTAACACA+AGG + chr3_2:66013029-66013048 Msa0418310:intron 20.0%
!!! TTTTTCTATGATAACTTTCT+TGG + chr3_2:66013518-66013537 Msa0418310:CDS 20.0%
!! CAAAAAAACAAAAATCTTTC+TGG + chr3_2:66012397-66012416 Msa0418310:intron 20.0%
!! TATCAGAAAAATAACACATA+AGG - chr3_2:66012472-66012491 None:intergenic 20.0%
!! TAAAGTTGAGAGAATAAAAA+TGG - chr3_2:66012504-66012523 None:intergenic 20.0%
!!! GATTTTTCTTTGTTATAGAT+TGG + chr3_2:66012602-66012621 Msa0418310:intron 20.0%
!! TTGTTAAAATGTTTAACACA+AGG + chr3_2:66013029-66013048 Msa0418310:intron 20.0%
!!! TTTTTCTATGATAACTTTCT+TGG + chr3_2:66013518-66013537 Msa0418310:CDS 20.0%
! AGTACTAAGTTAGAACAAAA+AGG - chr3_2:66012321-66012340 None:intergenic 25.0%
! TTGTTATAGATTGGATTTGA+TGG + chr3_2:66012611-66012630 Msa0418310:intron 25.0%
! TGTTATAGATTGGATTTGAT+GGG + chr3_2:66012612-66012631 Msa0418310:intron 25.0%
! AAGAGATAGATATTGAAGAA+TGG + chr3_2:66012784-66012803 Msa0418310:CDS 25.0%
!! TCTAAAATGAAGTAAGACTA+TGG - chr3_2:66012957-66012976 None:intergenic 25.0%
!!! ATAGTCTTACTTCATTTTAG+AGG + chr3_2:66012956-66012975 Msa0418310:intron 25.0%
!! AATTAGTCTCAATTCAGTTA+AGG + chr3_2:66013101-66013120 Msa0418310:intron 25.0%
! ACACAAATTTGATCCATTAT+TGG - chr3_2:66013176-66013195 None:intergenic 25.0%
! AGTACTAAGTTAGAACAAAA+AGG - chr3_2:66012321-66012340 None:intergenic 25.0%
! TTGTTATAGATTGGATTTGA+TGG + chr3_2:66012611-66012630 Msa0418310:intron 25.0%
! TGTTATAGATTGGATTTGAT+GGG + chr3_2:66012612-66012631 Msa0418310:intron 25.0%
! AAGAGATAGATATTGAAGAA+TGG + chr3_2:66012784-66012803 Msa0418310:CDS 25.0%
!! TCTAAAATGAAGTAAGACTA+TGG - chr3_2:66012957-66012976 None:intergenic 25.0%
!!! ATAGTCTTACTTCATTTTAG+AGG + chr3_2:66012956-66012975 Msa0418310:intron 25.0%
!! AATTAGTCTCAATTCAGTTA+AGG + chr3_2:66013101-66013120 Msa0418310:intron 25.0%
! ACACAAATTTGATCCATTAT+TGG - chr3_2:66013176-66013195 None:intergenic 25.0%
!! CTTTACTCTTGTTCTTGTTT+TGG + chr3_2:66012088-66012107 Msa0418310:five_prime_UTR 30.0%
!! TGTATTGAAGCAAAAGTGTT+AGG + chr3_2:66012119-66012138 Msa0418310:five_prime_UTR 30.0%
! AATTGATTCCTGATGCATTA+GGG + chr3_2:66012664-66012683 Msa0418310:CDS 30.0%
ATGCTTATCAGTAAAAGTTG+TGG + chr3_2:66012849-66012868 Msa0418310:CDS 30.0%
TTCAGCAATGAAAGTGAAAA+TGG - chr3_2:66012916-66012935 None:intergenic 30.0%
! ACTTGATTTTTGAATGCTGA+TGG + chr3_2:66013065-66013084 Msa0418310:intron 30.0%
AATGCTGATGGTTCATATTA+AGG + chr3_2:66013077-66013096 Msa0418310:intron 30.0%
ACATGTATCTAGTCCAATAA+TGG + chr3_2:66013160-66013179 Msa0418310:intron 30.0%
AGTGTTAAAGATGCGAAAAT+TGG + chr3_2:66013338-66013357 Msa0418310:intron 30.0%
!!! TTATACTCTTTTTCAAGTGC+TGG + chr3_2:66013426-66013445 Msa0418310:intron 30.0%
GTGAGCTATTGTATCAAAAT+TGG + chr3_2:66013543-66013562 Msa0418310:CDS 30.0%
ATTTCTTGTAGTGTATCCAA+TGG - chr3_2:66013636-66013655 None:intergenic 30.0%
AAGAAATCATCCATTTAGCT+AGG + chr3_2:66013649-66013668 Msa0418310:CDS 30.0%
AGGTATAATTCCTAGCTAAA+TGG - chr3_2:66013662-66013681 None:intergenic 30.0%
TTTAGCTAGGAATTATACCT+AGG + chr3_2:66013662-66013681 Msa0418310:CDS 30.0%
!! CTTTACTCTTGTTCTTGTTT+TGG + chr3_2:66012088-66012107 Msa0418310:five_prime_UTR 30.0%
!! TGTATTGAAGCAAAAGTGTT+AGG + chr3_2:66012119-66012138 Msa0418310:five_prime_UTR 30.0%
! AATTGATTCCTGATGCATTA+GGG + chr3_2:66012664-66012683 Msa0418310:CDS 30.0%
ATGCTTATCAGTAAAAGTTG+TGG + chr3_2:66012849-66012868 Msa0418310:CDS 30.0%
TTCAGCAATGAAAGTGAAAA+TGG - chr3_2:66012916-66012935 None:intergenic 30.0%
! ACTTGATTTTTGAATGCTGA+TGG + chr3_2:66013065-66013084 Msa0418310:intron 30.0%
AATGCTGATGGTTCATATTA+AGG + chr3_2:66013077-66013096 Msa0418310:intron 30.0%
ACATGTATCTAGTCCAATAA+TGG + chr3_2:66013160-66013179 Msa0418310:intron 30.0%
AGTGTTAAAGATGCGAAAAT+TGG + chr3_2:66013338-66013357 Msa0418310:intron 30.0%
!!! TTATACTCTTTTTCAAGTGC+TGG + chr3_2:66013426-66013445 Msa0418310:intron 30.0%
GTGAGCTATTGTATCAAAAT+TGG + chr3_2:66013543-66013562 Msa0418310:CDS 30.0%
ATTTCTTGTAGTGTATCCAA+TGG - chr3_2:66013636-66013655 None:intergenic 30.0%
AAGAAATCATCCATTTAGCT+AGG + chr3_2:66013649-66013668 Msa0418310:CDS 30.0%
AGGTATAATTCCTAGCTAAA+TGG - chr3_2:66013662-66013681 None:intergenic 30.0%
TTTAGCTAGGAATTATACCT+AGG + chr3_2:66013662-66013681 Msa0418310:CDS 30.0%
!!! TACTCTTGTTCTTGTTTTGG+TGG + chr3_2:66012091-66012110 Msa0418310:five_prime_UTR 35.0%
!! ATTGAAGCAAAAGTGTTAGG+TGG + chr3_2:66012122-66012141 Msa0418310:five_prime_UTR 35.0%
GAAGAAGGTGAAGATGAAAA+AGG - chr3_2:66012200-66012219 None:intergenic 35.0%
GGAAAGAAGAGATAAGAAGA+AGG - chr3_2:66012215-66012234 None:intergenic 35.0%
GAAACAAGAACCGAAAAATC+CGG - chr3_2:66012250-66012269 None:intergenic 35.0%
! GAATTGATTCCTGATGCATT+AGG + chr3_2:66012663-66012682 Msa0418310:CDS 35.0%
! ATTGATTCCTGATGCATTAG+GGG + chr3_2:66012665-66012684 Msa0418310:CDS 35.0%
! TTCCAAGGGTATGTAAATCA+TGG + chr3_2:66012730-66012749 Msa0418310:CDS 35.0%
TCTATCTCTTGCCAACAATT+AGG - chr3_2:66012774-66012793 None:intergenic 35.0%
CAGTTAAGGTGATACTACTA+TGG + chr3_2:66013115-66013134 Msa0418310:intron 35.0%
!!! TACTCTTGTTCTTGTTTTGG+TGG + chr3_2:66012091-66012110 Msa0418310:five_prime_UTR 35.0%
!! ATTGAAGCAAAAGTGTTAGG+TGG + chr3_2:66012122-66012141 Msa0418310:five_prime_UTR 35.0%
GAAGAAGGTGAAGATGAAAA+AGG - chr3_2:66012200-66012219 None:intergenic 35.0%
GGAAAGAAGAGATAAGAAGA+AGG - chr3_2:66012215-66012234 None:intergenic 35.0%
GAAACAAGAACCGAAAAATC+CGG - chr3_2:66012250-66012269 None:intergenic 35.0%
! GAATTGATTCCTGATGCATT+AGG + chr3_2:66012663-66012682 Msa0418310:CDS 35.0%
! ATTGATTCCTGATGCATTAG+GGG + chr3_2:66012665-66012684 Msa0418310:CDS 35.0%
! TTCCAAGGGTATGTAAATCA+TGG + chr3_2:66012730-66012749 Msa0418310:CDS 35.0%
TCTATCTCTTGCCAACAATT+AGG - chr3_2:66012774-66012793 None:intergenic 35.0%
CAGTTAAGGTGATACTACTA+TGG + chr3_2:66013115-66013134 Msa0418310:intron 35.0%
!!! CTTGTTCTTGTTTTGGTGGT+TGG + chr3_2:66012095-66012114 Msa0418310:five_prime_UTR 40.0%
TTGTTCGAGGAGGTGAATTA+TGG - chr3_2:66012272-66012291 None:intergenic 40.0%
!! TACAAGAGAGTGTTTGTTCG+AGG - chr3_2:66012285-66012304 None:intergenic 40.0%
AAGCAGGTGAATTACGAAGT+TGG + chr3_2:66012637-66012656 Msa0418310:CDS 40.0%
AGCAGGTGAATTACGAAGTT+GGG + chr3_2:66012638-66012657 Msa0418310:CDS 40.0%
CACCATGATTTACATACCCT+TGG - chr3_2:66012735-66012754 None:intergenic 40.0%
TCATGGTGTAAAGCAGTTTC+TGG + chr3_2:66012747-66012766 Msa0418310:CDS 40.0%
! CAGTTTCTGGACCTAATTGT+TGG + chr3_2:66012760-66012779 Msa0418310:CDS 40.0%
TCTGATCAAGTTTGTCAGGT+TGG - chr3_2:66012821-66012840 None:intergenic 40.0%
AGCATCTGATCAAGTTTGTC+AGG - chr3_2:66012825-66012844 None:intergenic 40.0%
AAATCGCTCATATTGCTCCT+TGG - chr3_2:66013474-66013493 None:intergenic 40.0%
AAGGAGCAATATGAGCGATT+TGG + chr3_2:66013473-66013492 Msa0418310:CDS 40.0%
!! CAATATGAGCGATTTGGTTG+TGG + chr3_2:66013479-66013498 Msa0418310:CDS 40.0%
AACTGCTAGAAGAGTTGTGT+AGG + chr3_2:66013601-66013620 Msa0418310:CDS 40.0%
GTGTAGGAATATGCATCCAT+TGG + chr3_2:66013617-66013636 Msa0418310:CDS 40.0%
GGAATTATACCTAGGAATCC+AGG + chr3_2:66013670-66013689 Msa0418310:exon 40.0%
CTTACCTAGGAAACTTTCCT+CGG - chr3_2:66013719-66013738 None:intergenic 40.0%
ACGGTCAAATATGCTTACCT+AGG - chr3_2:66013732-66013751 None:intergenic 40.0%
!!! CTTGTTCTTGTTTTGGTGGT+TGG + chr3_2:66012095-66012114 Msa0418310:five_prime_UTR 40.0%
TTGTTCGAGGAGGTGAATTA+TGG - chr3_2:66012272-66012291 None:intergenic 40.0%
!! TACAAGAGAGTGTTTGTTCG+AGG - chr3_2:66012285-66012304 None:intergenic 40.0%
AAGCAGGTGAATTACGAAGT+TGG + chr3_2:66012637-66012656 Msa0418310:CDS 40.0%
AGCAGGTGAATTACGAAGTT+GGG + chr3_2:66012638-66012657 Msa0418310:CDS 40.0%
CACCATGATTTACATACCCT+TGG - chr3_2:66012735-66012754 None:intergenic 40.0%
TCATGGTGTAAAGCAGTTTC+TGG + chr3_2:66012747-66012766 Msa0418310:CDS 40.0%
! CAGTTTCTGGACCTAATTGT+TGG + chr3_2:66012760-66012779 Msa0418310:CDS 40.0%
TCTGATCAAGTTTGTCAGGT+TGG - chr3_2:66012821-66012840 None:intergenic 40.0%
AGCATCTGATCAAGTTTGTC+AGG - chr3_2:66012825-66012844 None:intergenic 40.0%
AAATCGCTCATATTGCTCCT+TGG - chr3_2:66013474-66013493 None:intergenic 40.0%
AAGGAGCAATATGAGCGATT+TGG + chr3_2:66013473-66013492 Msa0418310:CDS 40.0%
!! CAATATGAGCGATTTGGTTG+TGG + chr3_2:66013479-66013498 Msa0418310:CDS 40.0%
AACTGCTAGAAGAGTTGTGT+AGG + chr3_2:66013601-66013620 Msa0418310:CDS 40.0%
GTGTAGGAATATGCATCCAT+TGG + chr3_2:66013617-66013636 Msa0418310:CDS 40.0%
GGAATTATACCTAGGAATCC+AGG + chr3_2:66013670-66013689 Msa0418310:exon 40.0%
CTTACCTAGGAAACTTTCCT+CGG - chr3_2:66013719-66013738 None:intergenic 40.0%
ACGGTCAAATATGCTTACCT+AGG - chr3_2:66013732-66013751 None:intergenic 40.0%
AAAAATCCGGCACATGCAAC+GGG - chr3_2:66012237-66012256 None:intergenic 45.0%
GAAAAATCCGGCACATGCAA+CGG - chr3_2:66012238-66012257 None:intergenic 45.0%
! GTTGCATGTGCCGGATTTTT+CGG + chr3_2:66012237-66012256 Msa0418310:five_prime_UTR 45.0%
!! AAGAGAGTGTTTGTTCGAGG+AGG - chr3_2:66012282-66012301 None:intergenic 45.0%
TTGGATTTGATGGGAGAAGC+AGG + chr3_2:66012621-66012640 Msa0418310:exon 45.0%
AAGATCACCCCTAATGCATC+AGG - chr3_2:66012675-66012694 None:intergenic 45.0%
AGAGAGTGACAGTGATTCCA+AGG + chr3_2:66012715-66012734 Msa0418310:CDS 45.0%
GAGAGTGACAGTGATTCCAA+GGG + chr3_2:66012716-66012735 Msa0418310:CDS 45.0%
CTCAGAAAGCTGTGTGTTTC+TGG + chr3_2:66012876-66012895 Msa0418310:CDS 45.0%
!! AGTCCAAAGAGAGCATCTCT+TGG + chr3_2:66013215-66013234 Msa0418310:intron 45.0%
AGTCCAAGAGATGCTCTCTT+TGG - chr3_2:66013221-66013240 None:intergenic 45.0%
! AGAGCATCTCTTGGACTAGA+AGG + chr3_2:66013224-66013243 Msa0418310:intron 45.0%
!! TTTCAAGTGCTGGTTCCCTT+CGG + chr3_2:66013436-66013455 Msa0418310:intron 45.0%
TGGTAGTCTCAAACTCCGAA+GGG - chr3_2:66013454-66013473 None:intergenic 45.0%
! TTGGTAGTCTCAAACTCCGA+AGG - chr3_2:66013455-66013474 None:intergenic 45.0%
! TTCGGAGTTTGAGACTACCA+AGG + chr3_2:66013454-66013473 Msa0418310:CDS 45.0%
TTGGTTGTGGAACAGATTGC+TGG + chr3_2:66013492-66013511 Msa0418310:CDS 45.0%
GCGTGTGCAATTGAGATGAT+AGG + chr3_2:66013567-66013586 Msa0418310:CDS 45.0%
ATTATCTGTAGCGAGGAACC+TGG - chr3_2:66013691-66013710 None:intergenic 45.0%
GGTATGCATTATCTGTAGCG+AGG - chr3_2:66013698-66013717 None:intergenic 45.0%
GCTACAGATAATGCATACCG+AGG + chr3_2:66013699-66013718 Msa0418310:three_prime_UTR 45.0%
CATACCGAGGAAAGTTTCCT+AGG + chr3_2:66013712-66013731 Msa0418310:three_prime_UTR 45.0%
CCTAGCTAAGCATGGTCAAA+CGG - chr3_2:66013751-66013770 None:intergenic 45.0%
GACCATGCTTAGCTAGGAAA+TGG + chr3_2:66013754-66013773 Msa0418310:three_prime_UTR 45.0%
CTCCATTTCCTAGCTAAGCA+TGG - chr3_2:66013759-66013778 None:intergenic 45.0%
CATGCTTAGCTAGGAAATGG+AGG + chr3_2:66013757-66013776 Msa0418310:three_prime_UTR 45.0%
AAAAATCCGGCACATGCAAC+GGG - chr3_2:66012237-66012256 None:intergenic 45.0%
GAAAAATCCGGCACATGCAA+CGG - chr3_2:66012238-66012257 None:intergenic 45.0%
! GTTGCATGTGCCGGATTTTT+CGG + chr3_2:66012237-66012256 Msa0418310:five_prime_UTR 45.0%
!! AAGAGAGTGTTTGTTCGAGG+AGG - chr3_2:66012282-66012301 None:intergenic 45.0%
TTGGATTTGATGGGAGAAGC+AGG + chr3_2:66012621-66012640 Msa0418310:exon 45.0%
AAGATCACCCCTAATGCATC+AGG - chr3_2:66012675-66012694 None:intergenic 45.0%
AGAGAGTGACAGTGATTCCA+AGG + chr3_2:66012715-66012734 Msa0418310:CDS 45.0%
GAGAGTGACAGTGATTCCAA+GGG + chr3_2:66012716-66012735 Msa0418310:CDS 45.0%
CTCAGAAAGCTGTGTGTTTC+TGG + chr3_2:66012876-66012895 Msa0418310:CDS 45.0%
!! AGTCCAAAGAGAGCATCTCT+TGG + chr3_2:66013215-66013234 Msa0418310:intron 45.0%
AGTCCAAGAGATGCTCTCTT+TGG - chr3_2:66013221-66013240 None:intergenic 45.0%
! AGAGCATCTCTTGGACTAGA+AGG + chr3_2:66013224-66013243 Msa0418310:intron 45.0%
!! TTTCAAGTGCTGGTTCCCTT+CGG + chr3_2:66013436-66013455 Msa0418310:intron 45.0%
TGGTAGTCTCAAACTCCGAA+GGG - chr3_2:66013454-66013473 None:intergenic 45.0%
! TTGGTAGTCTCAAACTCCGA+AGG - chr3_2:66013455-66013474 None:intergenic 45.0%
! TTCGGAGTTTGAGACTACCA+AGG + chr3_2:66013454-66013473 Msa0418310:CDS 45.0%
TTGGTTGTGGAACAGATTGC+TGG + chr3_2:66013492-66013511 Msa0418310:CDS 45.0%
GCGTGTGCAATTGAGATGAT+AGG + chr3_2:66013567-66013586 Msa0418310:CDS 45.0%
ATTATCTGTAGCGAGGAACC+TGG - chr3_2:66013691-66013710 None:intergenic 45.0%
GGTATGCATTATCTGTAGCG+AGG - chr3_2:66013698-66013717 None:intergenic 45.0%
GCTACAGATAATGCATACCG+AGG + chr3_2:66013699-66013718 Msa0418310:three_prime_UTR 45.0%
CATACCGAGGAAAGTTTCCT+AGG + chr3_2:66013712-66013731 Msa0418310:three_prime_UTR 45.0%
CCTAGCTAAGCATGGTCAAA+CGG - chr3_2:66013751-66013770 None:intergenic 45.0%
GACCATGCTTAGCTAGGAAA+TGG + chr3_2:66013754-66013773 Msa0418310:three_prime_UTR 45.0%
CTCCATTTCCTAGCTAAGCA+TGG - chr3_2:66013759-66013778 None:intergenic 45.0%
CATGCTTAGCTAGGAAATGG+AGG + chr3_2:66013757-66013776 Msa0418310:three_prime_UTR 45.0%
AAAATCCGGCACATGCAACG+GGG - chr3_2:66012236-66012255 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr3_2:66012363-66012382 None:intergenic 50.0%
CCGTTTGACCATGCTTAGCT+AGG + chr3_2:66013748-66013767 Msa0418310:three_prime_UTR 50.0%
GCTTAGCTAGGAAATGGAGG+TGG + chr3_2:66013760-66013779 Msa0418310:three_prime_UTR 50.0%
AAAATCCGGCACATGCAACG+GGG - chr3_2:66012236-66012255 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr3_2:66012363-66012382 None:intergenic 50.0%
CCGTTTGACCATGCTTAGCT+AGG + chr3_2:66013748-66013767 Msa0418310:three_prime_UTR 50.0%
GCTTAGCTAGGAAATGGAGG+TGG + chr3_2:66013760-66013779 Msa0418310:three_prime_UTR 50.0%
TCTTTCCCCGTTGCATGTGC+CGG + chr3_2:66012228-66012247 Msa0418310:five_prime_UTR 55.0%
AGCGAGGAACCTGGATTCCT+AGG - chr3_2:66013682-66013701 None:intergenic 55.0%
GGAAATGGAGGTGGTTACCG+AGG + chr3_2:66013769-66013788 Msa0418310:three_prime_UTR 55.0%
TCTTTCCCCGTTGCATGTGC+CGG + chr3_2:66012228-66012247 Msa0418310:five_prime_UTR 55.0%
AGCGAGGAACCTGGATTCCT+AGG - chr3_2:66013682-66013701 None:intergenic 55.0%
GGAAATGGAGGTGGTTACCG+AGG + chr3_2:66013769-66013788 Msa0418310:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 66012079 66013799 66012079 ID=Msa0418310;Name=Msa0418310
chr3_2 mRNA 66012079 66013799 66012079 ID=Msa0418310-mRNA-1;Parent=Msa0418310;Name=Msa0418310-mRNA-1;_AED=0.37;_eAED=0.37;_QI=233|1|1|1|1|1|3|116|182
chr3_2 exon 66012079 66012301 66012079 ID=Msa0418310-mRNA-1:exon:14939;Parent=Msa0418310-mRNA-1
chr3_2 exon 66012620 66012937 66012620 ID=Msa0418310-mRNA-1:exon:14940;Parent=Msa0418310-mRNA-1
chr3_2 exon 66013443 66013799 66013443 ID=Msa0418310-mRNA-1:exon:14941;Parent=Msa0418310-mRNA-1
chr3_2 five_prime_UTR 66012079 66012301 66012079 ID=Msa0418310-mRNA-1:five_prime_utr;Parent=Msa0418310-mRNA-1
chr3_2 five_prime_UTR 66012620 66012629 66012620 ID=Msa0418310-mRNA-1:five_prime_utr;Parent=Msa0418310-mRNA-1
chr3_2 CDS 66012630 66012937 66012630 ID=Msa0418310-mRNA-1:cds;Parent=Msa0418310-mRNA-1
chr3_2 CDS 66013443 66013683 66013443 ID=Msa0418310-mRNA-1:cds;Parent=Msa0418310-mRNA-1
chr3_2 three_prime_UTR 66013684 66013799 66013684 ID=Msa0418310-mRNA-1:three_prime_utr;Parent=Msa0418310-mRNA-1
Gene Sequence

>Msa0418310

ATGGGAGAAGCAGGTGAATTACGAAGTTGGGATGAATTGATTCCTGATGCATTAGGGGTGATCTTTACAAATCTTTCTCTGAAAGAGAGAGTGACAGTGATTCCAAGGGTATGTAAATCATGGTGTAAAGCAGTTTCTGGACCTAATTGTTGGCAAGAGATAGATATTGAAGAATGGAGCAACTATTGCCAACCTGACAAACTTGATCAGATGCTTCATATGCTTATCAGTAAAAGTTGTGGATCTCTCAGAAAGCTGTGTGTTTCTGGTCTTCAAACTGAGACCATTTTCACTTTCATTGCTGAAAATGCTGGTTCCCTTCGGAGTTTGAGACTACCAAGGAGCAATATGAGCGATTTGGTTGTGGAACAGATTGCTGGAAATTTTTCTATGATAACTTTCTTGGATGTGAGCTATTGTATCAAAATTGGTGCGTGTGCAATTGAGATGATAGGCAAGAACTGCAAACTGCTAGAAGAGTTGTGTAGGAATATGCATCCATTGGATACACTACAAGAAATCATCCATTTAGCTAGGAATTATACCTAG
Protein sequence

>Msa0418310

MGEAGELRSWDELIPDALGVIFTNLSLKERVTVIPRVCKSWCKAVSGPNCWQEIDIEEWSNYCQPDKLDQMLHMLISKSCGSLRKLCVSGLQTETIFTFIAENAGSLRSLRLPRSNMSDLVVEQIAGNFSMITFLDVSYCIKIGACAIEMIGKNCKLLEELCRNMHPLDTLQEIIHLARNYT*