AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0418640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418640 MtrunA17_Chr3g0116431 96.689 151 5 0 1 151 361 511 1.60e-106 313
Msa0418640 MtrunA17_Chr3g0116431 33.636 110 69 1 29 138 207 312 8.49e-17 76.3
Msa0418640 MtrunA17_Chr3g0116431 39.130 92 55 1 24 114 155 246 5.70e-16 73.9
Msa0418640 MtrunA17_Chr4g0058011 70.395 152 43 2 1 151 419 569 4.41e-72 226
Msa0418640 MtrunA17_Chr4g0058011 37.500 112 65 2 31 142 271 377 3.10e-16 74.7
Msa0418640 MtrunA17_Chr4g0058011 41.860 86 47 2 39 121 230 315 1.11e-14 70.1
Msa0418640 MtrunA17_Chr7g0249221 45.833 120 57 1 40 151 275 394 3.25e-33 120
Msa0418640 MtrunA17_Chr7g0249221 51.948 77 37 0 39 115 71 147 6.01e-22 90.5
Msa0418640 MtrunA17_Chr7g0249221 42.105 76 43 1 40 115 27 101 9.64e-17 75.9
Msa0418640 MtrunA17_Chr5g0438891 43.478 115 52 3 16 123 242 350 8.83e-27 103
Msa0418640 MtrunA17_Chr5g0438891 52.564 78 36 1 38 115 76 152 1.29e-21 89.4
Msa0418640 MtrunA17_Chr5g0438891 42.105 76 40 1 40 115 36 107 4.48e-16 73.9
Msa0418640 MtrunA17_Chr4g0066941 55.696 79 33 1 39 117 331 407 2.70e-24 97.4
Msa0418640 MtrunA17_Chr4g0066941 46.753 77 41 0 39 115 87 163 8.04e-21 87.8
Msa0418640 MtrunA17_Chr4g0066941 43.590 78 43 1 38 115 41 117 1.49e-17 78.2
Msa0418640 MtrunA17_Chr1g0195891 41.880 117 63 2 39 151 90 205 1.71e-22 91.3
Msa0418640 MtrunA17_Chr1g0195891 38.272 81 49 1 37 117 43 122 2.26e-13 66.2
Msa0418640 MtrunA17_Chr1g0195891 54.902 51 21 1 40 90 295 343 3.42e-13 65.5
Msa0418640 MtrunA17_Chr2g0286151 46.914 81 42 1 37 116 87 167 1.19e-21 90.1
Msa0418640 MtrunA17_Chr2g0286151 42.308 78 45 0 38 115 42 119 2.26e-17 77.8
Msa0418640 MtrunA17_Chr2g0286151 45.570 79 43 0 38 116 301 379 4.33e-17 77.0
Msa0418640 MtrunA17_Chr8g0356241 39.252 107 54 2 40 142 395 494 1.45e-20 87.0
Msa0418640 MtrunA17_Chr8g0356241 43.119 109 55 2 35 141 185 288 1.16e-19 84.3
Msa0418640 MtrunA17_Chr8g0356241 38.372 86 46 2 40 119 138 222 9.26e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418640 AT3G48440.1 59.677 124 48 2 1 122 302 425 9.00e-48 160
Msa0418640 AT3G48440.1 33.600 125 76 3 3 125 118 237 5.39e-14 68.6
Msa0418640 AT3G48440.1 41.975 81 41 2 40 116 107 185 2.32e-13 66.6
Msa0418640 AT5G63260.1 51.389 144 62 2 7 150 298 433 2.49e-44 150
Msa0418640 AT5G63260.1 39.080 87 53 0 29 115 134 220 3.94e-14 68.9
Msa0418640 AT5G63260.2 51.389 144 62 2 7 150 314 449 2.69e-44 151
Msa0418640 AT5G63260.2 39.773 88 53 0 28 115 149 236 5.00e-15 71.6
Msa0418640 AT1G48195.1 64.935 77 27 0 38 114 4 80 1.99e-34 115
Msa0418640 AT5G16540.3 65.823 79 27 0 40 118 219 297 2.67e-34 123
Msa0418640 AT5G16540.3 49.351 77 39 0 39 115 62 138 2.91e-21 88.2
Msa0418640 AT5G16540.3 46.835 79 39 2 40 117 18 94 1.35e-15 72.8
Msa0418640 AT5G16540.1 65.823 79 27 0 40 118 240 318 2.88e-34 123
Msa0418640 AT5G16540.1 49.351 77 39 0 39 115 83 159 2.53e-21 88.6
Msa0418640 AT5G16540.1 46.835 79 39 2 40 117 39 115 1.16e-15 73.2
Msa0418640 AT3G02830.2 64.557 79 28 0 40 118 251 329 1.70e-33 121
Msa0418640 AT3G02830.2 45.545 101 54 1 39 138 61 161 1.20e-20 87.0
Msa0418640 AT3G02830.2 44.872 78 42 1 40 117 17 93 4.26e-18 79.7
Msa0418640 AT3G02830.1 64.557 79 28 0 40 118 271 349 1.72e-33 121
Msa0418640 AT3G02830.1 45.545 101 54 1 39 138 81 181 1.15e-20 87.0
Msa0418640 AT3G02830.1 44.872 78 42 1 40 117 37 113 4.25e-18 80.1
Msa0418640 AT2G47850.4 60.000 80 32 0 38 117 162 241 3.25e-30 112
Msa0418640 AT2G47850.2 60.000 80 32 0 38 117 258 337 1.16e-29 112
Msa0418640 AT2G47850.2 36.250 80 50 1 38 117 40 118 7.43e-15 70.9
Msa0418640 AT2G47850.2 52.941 51 24 0 39 89 86 136 5.45e-11 59.7
Msa0418640 AT2G47850.1 60.000 80 32 0 38 117 284 363 2.39e-29 111
Msa0418640 AT2G47850.1 49.425 87 40 1 39 121 86 172 3.29e-21 89.0
Msa0418640 AT2G47850.3 60.000 80 32 0 38 117 284 363 2.39e-29 111
Msa0418640 AT2G47850.3 49.425 87 40 1 39 121 86 172 3.29e-21 89.0
Msa0418640 AT3G06410.1 43.860 114 59 1 6 114 265 378 1.89e-27 106
Msa0418640 AT3G06410.1 39.394 99 60 0 17 115 28 126 1.20e-19 84.7
Msa0418640 AT3G06410.1 46.914 81 41 1 40 118 97 177 1.43e-19 84.3
Msa0418640 AT5G16540.2 58.228 79 26 1 40 118 240 311 3.68e-27 104
Msa0418640 AT5G16540.2 49.351 77 39 0 39 115 83 159 2.38e-21 88.6
Msa0418640 AT5G16540.2 46.835 79 39 2 40 117 39 115 1.11e-15 73.2
Msa0418640 AT5G18550.1 55.844 77 33 1 38 114 295 370 3.72e-25 100
Msa0418640 AT5G18550.1 46.429 84 43 1 35 116 89 172 2.79e-21 89.4
Msa0418640 AT5G18550.1 50.000 78 39 0 38 115 46 123 6.49e-21 88.2
Msa0418640 AT5G18550.4 55.844 77 33 1 38 114 295 370 4.16e-25 99.8
Msa0418640 AT5G18550.4 46.429 84 43 1 35 116 89 172 3.28e-21 89.0
Msa0418640 AT5G18550.4 50.000 78 39 0 38 115 46 123 7.44e-21 87.8
Msa0418640 AT5G18550.3 55.844 77 33 1 38 114 263 338 4.31e-25 99.8
Msa0418640 AT5G18550.3 46.429 84 43 1 35 116 57 140 4.08e-21 88.6
Msa0418640 AT5G18550.3 50.000 78 39 0 38 115 14 91 8.24e-21 87.8
Msa0418640 AT5G16540.4 49.351 77 39 0 39 115 62 138 1.63e-21 87.8
Msa0418640 AT5G16540.4 68.627 51 16 0 40 90 219 269 1.99e-18 79.7
Msa0418640 AT5G16540.4 46.835 79 39 2 40 117 18 94 1.15e-15 72.0
Msa0418640 AT5G18550.2 46.429 84 43 1 35 116 89 172 3.18e-21 89.0
Msa0418640 AT5G18550.2 50.000 78 39 0 38 115 46 123 7.43e-21 87.8
Msa0418640 AT5G18550.2 45.455 77 28 1 38 114 295 357 2.23e-18 80.9
Msa0418640 AT1G04990.3 48.780 82 41 1 42 123 179 259 1.07e-20 86.7
Msa0418640 AT1G04990.3 49.231 65 32 1 51 115 17 80 4.29e-14 68.6
Msa0418640 AT1G04990.5 51.282 78 37 1 38 115 85 161 1.61e-20 86.7
Msa0418640 AT1G04990.5 44.186 86 40 3 34 115 35 116 1.52e-14 70.1
Msa0418640 AT1G04990.5 39.024 82 29 2 42 123 260 320 5.24e-12 62.8
Msa0418640 AT1G04990.1 51.282 78 37 1 38 115 85 161 1.66e-20 86.7
Msa0418640 AT1G04990.1 48.780 82 41 1 42 123 260 340 2.53e-20 86.3
Msa0418640 AT1G04990.1 44.186 86 40 3 34 115 35 116 1.84e-14 69.7
Msa0418640 AT1G04990.6 51.282 78 37 1 38 115 85 161 1.66e-20 86.7
Msa0418640 AT1G04990.6 48.780 82 41 1 42 123 260 340 2.53e-20 86.3
Msa0418640 AT1G04990.6 44.186 86 40 3 34 115 35 116 1.84e-14 69.7
Msa0418640 AT1G04990.2 51.282 78 37 1 38 115 85 161 1.66e-20 86.7
Msa0418640 AT1G04990.2 48.780 82 41 1 42 123 260 340 2.53e-20 86.3
Msa0418640 AT1G04990.2 44.186 86 40 3 34 115 35 116 1.84e-14 69.7
Msa0418640 AT3G12680.1 44.578 83 39 1 40 115 417 499 2.70e-20 86.7
Msa0418640 AT3G12680.1 43.182 88 47 1 35 122 217 301 1.05e-18 82.0
Msa0418640 AT3G12680.1 32.143 140 64 2 2 115 232 366 4.20e-17 77.4
Msa0418640 AT2G32930.2 47.436 78 40 1 38 115 83 159 1.66e-19 84.3
Msa0418640 AT2G32930.2 49.315 73 37 0 48 120 270 342 9.01e-19 82.0
Msa0418640 AT2G32930.2 44.156 77 39 2 40 115 41 114 2.71e-15 72.4
Msa0418640 AT2G32930.3 47.436 78 40 1 38 115 56 132 1.82e-19 84.0
Msa0418640 AT2G32930.3 49.315 73 37 0 48 120 243 315 1.09e-18 81.6
Msa0418640 AT2G32930.3 44.156 77 39 2 40 115 14 87 3.53e-15 72.0
Msa0418640 AT2G32930.1 49.315 73 37 0 48 120 266 338 7.71e-19 82.4
Msa0418640 AT2G32930.1 43.590 78 39 2 38 115 83 155 4.42e-15 71.6
Msa0418640 AT2G32930.1 44.156 77 35 3 40 115 41 110 5.87e-13 65.5
Msa0418640 AT1G04990.4 49.231 65 32 1 51 115 17 80 4.15e-14 68.2
Msa0418640 AT1G04990.4 39.024 82 29 2 42 123 179 239 3.52e-12 62.8

Find 54 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGGTTCACCAGGTCTTTC+TGG 0.127881 3_2:-66428205 None:intergenic
CGATACGGAATTTGCAAATT+TGG 0.131640 3_2:+66428470 Msa0418640:CDS
CAAAAGAACTGCTCAATTAA+TGG 0.266115 3_2:+66428956 Msa0418640:three_prime_UTR
TTCCAGTTTGTTATCTCTCT+TGG 0.278370 3_2:+66428828 Msa0418640:three_prime_UTR
AAGATGAAAATCACAAAATA+TGG 0.299144 3_2:-66428690 None:intergenic
AGCTGCTGTAGATTATCTTT+TGG 0.303041 3_2:+66428805 Msa0418640:three_prime_UTR
TGTAATGAGTGCACACATTC+TGG 0.330211 3_2:-66428445 None:intergenic
TTTAAGAAGAAACTGCATTC+AGG 0.330498 3_2:-66428223 None:intergenic
TAAGCATTCGTTTCCATAGC+TGG 0.369326 3_2:-66428145 None:intergenic
ACTAAAGTTCAACATTTCAT+TGG 0.377225 3_2:-66428928 None:intergenic
TATCTCTCTTGGATTAGTAT+TGG 0.397975 3_2:+66428839 Msa0418640:three_prime_UTR
GATAACGTCCAGGCATAGTT+GGG 0.405460 3_2:-66428532 None:intergenic
ATTACTTTATTTAACTTCAC+TGG 0.413927 3_2:+66428720 Msa0418640:three_prime_UTR
CTTTCTGGAAACTCTTCAAC+TGG 0.426995 3_2:-66428190 None:intergenic
GCTGGATTGTTCACGGCATA+TGG 0.429292 3_2:-66428127 None:intergenic
TTCCATAGCTGGATTGTTCA+CGG 0.431515 3_2:-66428134 None:intergenic
CAAAAGGCTTGTGCCACATC+AGG 0.446756 3_2:-66428748 None:intergenic
AATTTCATCATCCAAAGAAC+CGG 0.447322 3_2:+66428278 Msa0418640:CDS
AAGGCAGACCCTTGTCACTT+AGG 0.458239 3_2:-66428324 None:intergenic
ATATATAGCTATTCCGCAAA+AGG 0.460385 3_2:-66428764 None:intergenic
CCCTTGTCACTTAGGTTGCA+TGG 0.470112 3_2:-66428316 None:intergenic
AGTATTGAGGAGGCTGGAAG+TGG 0.494418 3_2:+66428581 Msa0418640:CDS
ATCCAAGAGAGATAACAAAC+TGG 0.502672 3_2:-66428830 None:intergenic
GGATTTAAATTTGCAATCCC+CGG 0.510053 3_2:-66428248 None:intergenic
CAAATTTGCAAATTCCGTAT+CGG 0.515996 3_2:-66428469 None:intergenic
TGATAACGTCCAGGCATAGT+TGG 0.516040 3_2:-66428533 None:intergenic
CACAAACTCGGCTAGTATTG+AGG 0.521153 3_2:+66428568 Msa0418640:CDS
TGGATGATGAAATTTGCAAT+GGG 0.532458 3_2:-66428269 None:intergenic
TCGGCTAGTATTGAGGAGGC+TGG 0.532916 3_2:+66428575 Msa0418640:CDS
ATTGGATTATATAGTTGTGC+TGG 0.534752 3_2:+66428857 Msa0418640:three_prime_UTR
TTGGATGATGAAATTTGCAA+TGG 0.544786 3_2:-66428270 None:intergenic
TGCCGTGAACAATCCAGCTA+TGG 0.554540 3_2:+66428132 Msa0418640:CDS
TGCACTCATTACAACCGATA+CGG 0.555974 3_2:+66428455 Msa0418640:CDS
GGGGCGGTAGGTTTATCGGG+TGG 0.565245 3_2:-66428511 None:intergenic
ATTGAGGAGGCTGGAAGTGG+AGG 0.565408 3_2:+66428584 Msa0418640:CDS
ACAATCCTCTCACACAAACT+CGG 0.577005 3_2:+66428556 Msa0418640:CDS
ACTAGCCGAGTTTGTGTGAG+AGG 0.582786 3_2:-66428561 None:intergenic
TGAAAATCACAAAATATGGA+TGG 0.582977 3_2:-66428686 None:intergenic
AAGATAATCTACAGCAGCTG+AGG 0.586171 3_2:-66428801 None:intergenic
AAATAACTTACAGGTCTCAA+AGG 0.588071 3_2:-66428343 None:intergenic
AAACTGCATTCAGGTTCACC+AGG 0.589001 3_2:-66428214 None:intergenic
GAGGATTGTTGATAACGTCC+AGG 0.597566 3_2:-66428542 None:intergenic
GGGTGGTCATACTTACAAGC+TGG 0.604093 3_2:-66428494 None:intergenic
ATAACGTCCAGGCATAGTTG+GGG 0.618044 3_2:-66428531 None:intergenic
TTCACTGGTTATGCCTGATG+TGG 0.629637 3_2:+66428735 Msa0418640:three_prime_UTR
TTCACGGCATATGGTGAAGG+TGG 0.632618 3_2:-66428118 None:intergenic
TCCATGCAACCTAAGTGACA+AGG 0.634773 3_2:+66428315 Msa0418640:CDS
CAACTGGTGTATGCTTATGA+CGG 0.648143 3_2:-66428174 None:intergenic
TAACGTCCAGGCATAGTTGG+GGG 0.648794 3_2:-66428530 None:intergenic
TTGTTCACGGCATATGGTGA+AGG 0.648890 3_2:-66428121 None:intergenic
GAAGAGTTTCCAGAAAGACC+TGG 0.657339 3_2:+66428196 Msa0418640:CDS
CCATGCAACCTAAGTGACAA+GGG 0.676957 3_2:+66428316 Msa0418640:CDS
AAACTCGGCTAGTATTGAGG+AGG 0.710931 3_2:+66428571 Msa0418640:CDS
TTGTCACTTAGGTTGCATGG+AGG 0.747188 3_2:-66428313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCTTGTTTATGTATTA+TGG + chr3_2:66428902-66428921 Msa0418640:three_prime_UTR 15.0%
!!! ATTTTCTTGTTTATGTATTA+TGG + chr3_2:66428902-66428921 Msa0418640:three_prime_UTR 15.0%
!! TTTAATATCTTGTGAAATTC+AGG + chr3_2:66428421-66428440 Msa0418640:intron 20.0%
!! TTAATATCTTGTGAAATTCA+GGG + chr3_2:66428422-66428441 Msa0418640:intron 20.0%
!! AAGATGAAAATCACAAAATA+TGG - chr3_2:66428693-66428712 None:intergenic 20.0%
!!! ATTACTTTATTTAACTTCAC+TGG + chr3_2:66428720-66428739 Msa0418640:three_prime_UTR 20.0%
!! TTTAATATCTTGTGAAATTC+AGG + chr3_2:66428421-66428440 Msa0418640:intron 20.0%
!! TTAATATCTTGTGAAATTCA+GGG + chr3_2:66428422-66428441 Msa0418640:intron 20.0%
!! AAGATGAAAATCACAAAATA+TGG - chr3_2:66428693-66428712 None:intergenic 20.0%
!!! ATTACTTTATTTAACTTCAC+TGG + chr3_2:66428720-66428739 Msa0418640:three_prime_UTR 20.0%
! ATCAGAGTAAAATAACTTAC+AGG - chr3_2:66428355-66428374 None:intergenic 25.0%
! AAATGTTAGATCAAAGAAAC+TGG - chr3_2:66428404-66428423 None:intergenic 25.0%
!! GATTGAAACATTTTCTATCA+AGG + chr3_2:66428639-66428658 Msa0418640:three_prime_UTR 25.0%
!! ATTTTCTATCAAGGATCTTA+TGG + chr3_2:66428648-66428667 Msa0418640:three_prime_UTR 25.0%
! TGAAAATCACAAAATATGGA+TGG - chr3_2:66428689-66428708 None:intergenic 25.0%
! ACTAAAGTTCAACATTTCAT+TGG - chr3_2:66428931-66428950 None:intergenic 25.0%
!! TTTTCCTAAAATCTCTTAAC+AGG - chr3_2:66428994-66429013 None:intergenic 25.0%
!!! AAGAGATTTTAGGAAAAATC+AGG + chr3_2:66428997-66429016 Msa0418640:three_prime_UTR 25.0%
! ATCAGAGTAAAATAACTTAC+AGG - chr3_2:66428355-66428374 None:intergenic 25.0%
! AAATGTTAGATCAAAGAAAC+TGG - chr3_2:66428404-66428423 None:intergenic 25.0%
!! GATTGAAACATTTTCTATCA+AGG + chr3_2:66428639-66428658 Msa0418640:three_prime_UTR 25.0%
!! ATTTTCTATCAAGGATCTTA+TGG + chr3_2:66428648-66428667 Msa0418640:three_prime_UTR 25.0%
! TGAAAATCACAAAATATGGA+TGG - chr3_2:66428689-66428708 None:intergenic 25.0%
! ACTAAAGTTCAACATTTCAT+TGG - chr3_2:66428931-66428950 None:intergenic 25.0%
!! TTTTCCTAAAATCTCTTAAC+AGG - chr3_2:66428994-66429013 None:intergenic 25.0%
!!! AAGAGATTTTAGGAAAAATC+AGG + chr3_2:66428997-66429016 Msa0418640:three_prime_UTR 25.0%
TTTAAGAAGAAACTGCATTC+AGG - chr3_2:66428226-66428245 None:intergenic 30.0%
TGCAGTTTCTTCTTAAAAAC+CGG + chr3_2:66428229-66428248 Msa0418640:CDS 30.0%
TGGATGATGAAATTTGCAAT+GGG - chr3_2:66428272-66428291 None:intergenic 30.0%
TTGGATGATGAAATTTGCAA+TGG - chr3_2:66428273-66428292 None:intergenic 30.0%
AATTTCATCATCCAAAGAAC+CGG + chr3_2:66428278-66428297 Msa0418640:CDS 30.0%
! AATTTTGTAATCCGGTTCTT+TGG - chr3_2:66428292-66428311 None:intergenic 30.0%
! ATGGAGGTAATTTTGTAATC+CGG - chr3_2:66428300-66428319 None:intergenic 30.0%
AAATAACTTACAGGTCTCAA+AGG - chr3_2:66428346-66428365 None:intergenic 30.0%
CAAATTTGCAAATTCCGTAT+CGG - chr3_2:66428472-66428491 None:intergenic 30.0%
!! GCAACAATTTTATAGCTTTG+AGG + chr3_2:66428617-66428636 Msa0418640:exon 30.0%
ATATATAGCTATTCCGCAAA+AGG - chr3_2:66428767-66428786 None:intergenic 30.0%
!! TATCTCTCTTGGATTAGTAT+TGG + chr3_2:66428839-66428858 Msa0418640:three_prime_UTR 30.0%
ATTGGATTATATAGTTGTGC+TGG + chr3_2:66428857-66428876 Msa0418640:three_prime_UTR 30.0%
CAAAAGAACTGCTCAATTAA+TGG + chr3_2:66428956-66428975 Msa0418640:three_prime_UTR 30.0%
ACAAGTTATACATGACTTAC+TGG + chr3_2:66429043-66429062 Msa0418640:three_prime_UTR 30.0%
TTTAAGAAGAAACTGCATTC+AGG - chr3_2:66428226-66428245 None:intergenic 30.0%
TGCAGTTTCTTCTTAAAAAC+CGG + chr3_2:66428229-66428248 Msa0418640:CDS 30.0%
TGGATGATGAAATTTGCAAT+GGG - chr3_2:66428272-66428291 None:intergenic 30.0%
TTGGATGATGAAATTTGCAA+TGG - chr3_2:66428273-66428292 None:intergenic 30.0%
AATTTCATCATCCAAAGAAC+CGG + chr3_2:66428278-66428297 Msa0418640:CDS 30.0%
! AATTTTGTAATCCGGTTCTT+TGG - chr3_2:66428292-66428311 None:intergenic 30.0%
! ATGGAGGTAATTTTGTAATC+CGG - chr3_2:66428300-66428319 None:intergenic 30.0%
AAATAACTTACAGGTCTCAA+AGG - chr3_2:66428346-66428365 None:intergenic 30.0%
CAAATTTGCAAATTCCGTAT+CGG - chr3_2:66428472-66428491 None:intergenic 30.0%
!! GCAACAATTTTATAGCTTTG+AGG + chr3_2:66428617-66428636 Msa0418640:exon 30.0%
ATATATAGCTATTCCGCAAA+AGG - chr3_2:66428767-66428786 None:intergenic 30.0%
!! TATCTCTCTTGGATTAGTAT+TGG + chr3_2:66428839-66428858 Msa0418640:three_prime_UTR 30.0%
ATTGGATTATATAGTTGTGC+TGG + chr3_2:66428857-66428876 Msa0418640:three_prime_UTR 30.0%
CAAAAGAACTGCTCAATTAA+TGG + chr3_2:66428956-66428975 Msa0418640:three_prime_UTR 30.0%
!! CACCTTTCTATTTTTCTGAG+AGG + chr3_2:66428086-66428105 Msa0418640:CDS 35.0%
CTCCTCTCAGAAAAATAGAA+AGG - chr3_2:66428091-66428110 None:intergenic 35.0%
GCAGTTTCTTCTTAAAAACC+GGG + chr3_2:66428230-66428249 Msa0418640:CDS 35.0%
CAGTTTCTTCTTAAAAACCG+GGG + chr3_2:66428231-66428250 Msa0418640:CDS 35.0%
GGATTTAAATTTGCAATCCC+CGG - chr3_2:66428251-66428270 None:intergenic 35.0%
AACTGGTGACATGTACATTA+GGG - chr3_2:66428387-66428406 None:intergenic 35.0%
AAACTGGTGACATGTACATT+AGG - chr3_2:66428388-66428407 None:intergenic 35.0%
CGATACGGAATTTGCAAATT+TGG + chr3_2:66428470-66428489 Msa0418640:CDS 35.0%
! AGCTGCTGTAGATTATCTTT+TGG + chr3_2:66428805-66428824 Msa0418640:three_prime_UTR 35.0%
!! TTCCAGTTTGTTATCTCTCT+TGG + chr3_2:66428828-66428847 Msa0418640:three_prime_UTR 35.0%
ATCCAAGAGAGATAACAAAC+TGG - chr3_2:66428833-66428852 None:intergenic 35.0%
! GTAGCCTGTTAAGAGATTTT+AGG + chr3_2:66428987-66429006 Msa0418640:three_prime_UTR 35.0%
!! CACCTTTCTATTTTTCTGAG+AGG + chr3_2:66428086-66428105 Msa0418640:CDS 35.0%
CTCCTCTCAGAAAAATAGAA+AGG - chr3_2:66428091-66428110 None:intergenic 35.0%
GCAGTTTCTTCTTAAAAACC+GGG + chr3_2:66428230-66428249 Msa0418640:CDS 35.0%
CAGTTTCTTCTTAAAAACCG+GGG + chr3_2:66428231-66428250 Msa0418640:CDS 35.0%
GGATTTAAATTTGCAATCCC+CGG - chr3_2:66428251-66428270 None:intergenic 35.0%
AACTGGTGACATGTACATTA+GGG - chr3_2:66428387-66428406 None:intergenic 35.0%
AAACTGGTGACATGTACATT+AGG - chr3_2:66428388-66428407 None:intergenic 35.0%
CGATACGGAATTTGCAAATT+TGG + chr3_2:66428470-66428489 Msa0418640:CDS 35.0%
! AGCTGCTGTAGATTATCTTT+TGG + chr3_2:66428805-66428824 Msa0418640:three_prime_UTR 35.0%
!! TTCCAGTTTGTTATCTCTCT+TGG + chr3_2:66428828-66428847 Msa0418640:three_prime_UTR 35.0%
ATCCAAGAGAGATAACAAAC+TGG - chr3_2:66428833-66428852 None:intergenic 35.0%
! GTAGCCTGTTAAGAGATTTT+AGG + chr3_2:66428987-66429006 Msa0418640:three_prime_UTR 35.0%
TTCCATAGCTGGATTGTTCA+CGG - chr3_2:66428137-66428156 None:intergenic 40.0%
TAAGCATTCGTTTCCATAGC+TGG - chr3_2:66428148-66428167 None:intergenic 40.0%
CAACTGGTGTATGCTTATGA+CGG - chr3_2:66428177-66428196 None:intergenic 40.0%
! CTTTCTGGAAACTCTTCAAC+TGG - chr3_2:66428193-66428212 None:intergenic 40.0%
TGTAATGAGTGCACACATTC+TGG - chr3_2:66428448-66428467 None:intergenic 40.0%
TGCACTCATTACAACCGATA+CGG + chr3_2:66428455-66428474 Msa0418640:CDS 40.0%
ACAATCCTCTCACACAAACT+CGG + chr3_2:66428556-66428575 Msa0418640:CDS 40.0%
AAGATAATCTACAGCAGCTG+AGG - chr3_2:66428804-66428823 None:intergenic 40.0%
TTCCATAGCTGGATTGTTCA+CGG - chr3_2:66428137-66428156 None:intergenic 40.0%
TAAGCATTCGTTTCCATAGC+TGG - chr3_2:66428148-66428167 None:intergenic 40.0%
CAACTGGTGTATGCTTATGA+CGG - chr3_2:66428177-66428196 None:intergenic 40.0%
! CTTTCTGGAAACTCTTCAAC+TGG - chr3_2:66428193-66428212 None:intergenic 40.0%
TGTAATGAGTGCACACATTC+TGG - chr3_2:66428448-66428467 None:intergenic 40.0%
TGCACTCATTACAACCGATA+CGG + chr3_2:66428455-66428474 Msa0418640:CDS 40.0%
ACAATCCTCTCACACAAACT+CGG + chr3_2:66428556-66428575 Msa0418640:CDS 40.0%
AAGATAATCTACAGCAGCTG+AGG - chr3_2:66428804-66428823 None:intergenic 40.0%
TTGTTCACGGCATATGGTGA+AGG - chr3_2:66428124-66428143 None:intergenic 45.0%
GAAGAGTTTCCAGAAAGACC+TGG + chr3_2:66428196-66428215 Msa0418640:CDS 45.0%
! AAACTGCATTCAGGTTCACC+AGG - chr3_2:66428217-66428236 None:intergenic 45.0%
! TTGTCACTTAGGTTGCATGG+AGG - chr3_2:66428316-66428335 None:intergenic 45.0%
TCCATGCAACCTAAGTGACA+AGG + chr3_2:66428315-66428334 Msa0418640:CDS 45.0%
CCATGCAACCTAAGTGACAA+GGG + chr3_2:66428316-66428335 Msa0418640:CDS 45.0%
ATAACGTCCAGGCATAGTTG+GGG - chr3_2:66428534-66428553 None:intergenic 45.0%
GATAACGTCCAGGCATAGTT+GGG - chr3_2:66428535-66428554 None:intergenic 45.0%
! TGATAACGTCCAGGCATAGT+TGG - chr3_2:66428536-66428555 None:intergenic 45.0%
!! GAGGATTGTTGATAACGTCC+AGG - chr3_2:66428545-66428564 None:intergenic 45.0%
CACAAACTCGGCTAGTATTG+AGG + chr3_2:66428568-66428587 Msa0418640:CDS 45.0%
! AAACTCGGCTAGTATTGAGG+AGG + chr3_2:66428571-66428590 Msa0418640:CDS 45.0%
! TTCACTGGTTATGCCTGATG+TGG + chr3_2:66428735-66428754 Msa0418640:three_prime_UTR 45.0%
TTGTTCACGGCATATGGTGA+AGG - chr3_2:66428124-66428143 None:intergenic 45.0%
GAAGAGTTTCCAGAAAGACC+TGG + chr3_2:66428196-66428215 Msa0418640:CDS 45.0%
! AAACTGCATTCAGGTTCACC+AGG - chr3_2:66428217-66428236 None:intergenic 45.0%
! TTGTCACTTAGGTTGCATGG+AGG - chr3_2:66428316-66428335 None:intergenic 45.0%
TCCATGCAACCTAAGTGACA+AGG + chr3_2:66428315-66428334 Msa0418640:CDS 45.0%
CCATGCAACCTAAGTGACAA+GGG + chr3_2:66428316-66428335 Msa0418640:CDS 45.0%
ATAACGTCCAGGCATAGTTG+GGG - chr3_2:66428534-66428553 None:intergenic 45.0%
GATAACGTCCAGGCATAGTT+GGG - chr3_2:66428535-66428554 None:intergenic 45.0%
! TGATAACGTCCAGGCATAGT+TGG - chr3_2:66428536-66428555 None:intergenic 45.0%
!! GAGGATTGTTGATAACGTCC+AGG - chr3_2:66428545-66428564 None:intergenic 45.0%
CACAAACTCGGCTAGTATTG+AGG + chr3_2:66428568-66428587 Msa0418640:CDS 45.0%
! AAACTCGGCTAGTATTGAGG+AGG + chr3_2:66428571-66428590 Msa0418640:CDS 45.0%
! TTCACTGGTTATGCCTGATG+TGG + chr3_2:66428735-66428754 Msa0418640:three_prime_UTR 45.0%
TTCACGGCATATGGTGAAGG+TGG - chr3_2:66428121-66428140 None:intergenic 50.0%
GCTGGATTGTTCACGGCATA+TGG - chr3_2:66428130-66428149 None:intergenic 50.0%
TGCCGTGAACAATCCAGCTA+TGG + chr3_2:66428132-66428151 Msa0418640:CDS 50.0%
TCAGGTTCACCAGGTCTTTC+TGG - chr3_2:66428208-66428227 None:intergenic 50.0%
! CCCTTGTCACTTAGGTTGCA+TGG - chr3_2:66428319-66428338 None:intergenic 50.0%
! AAGGCAGACCCTTGTCACTT+AGG - chr3_2:66428327-66428346 None:intergenic 50.0%
GGGTGGTCATACTTACAAGC+TGG - chr3_2:66428497-66428516 None:intergenic 50.0%
TAACGTCCAGGCATAGTTGG+GGG - chr3_2:66428533-66428552 None:intergenic 50.0%
ACTAGCCGAGTTTGTGTGAG+AGG - chr3_2:66428564-66428583 None:intergenic 50.0%
! AGTATTGAGGAGGCTGGAAG+TGG + chr3_2:66428581-66428600 Msa0418640:CDS 50.0%
CAAAAGGCTTGTGCCACATC+AGG - chr3_2:66428751-66428770 None:intergenic 50.0%
! GATGTGGCACAAGCCTTTTG+CGG + chr3_2:66428751-66428770 Msa0418640:three_prime_UTR 50.0%
TTCACGGCATATGGTGAAGG+TGG - chr3_2:66428121-66428140 None:intergenic 50.0%
GCTGGATTGTTCACGGCATA+TGG - chr3_2:66428130-66428149 None:intergenic 50.0%
TGCCGTGAACAATCCAGCTA+TGG + chr3_2:66428132-66428151 Msa0418640:CDS 50.0%
TCAGGTTCACCAGGTCTTTC+TGG - chr3_2:66428208-66428227 None:intergenic 50.0%
! CCCTTGTCACTTAGGTTGCA+TGG - chr3_2:66428319-66428338 None:intergenic 50.0%
! AAGGCAGACCCTTGTCACTT+AGG - chr3_2:66428327-66428346 None:intergenic 50.0%
GGGTGGTCATACTTACAAGC+TGG - chr3_2:66428497-66428516 None:intergenic 50.0%
TAACGTCCAGGCATAGTTGG+GGG - chr3_2:66428533-66428552 None:intergenic 50.0%
ACTAGCCGAGTTTGTGTGAG+AGG - chr3_2:66428564-66428583 None:intergenic 50.0%
! AGTATTGAGGAGGCTGGAAG+TGG + chr3_2:66428581-66428600 Msa0418640:CDS 50.0%
CAAAAGGCTTGTGCCACATC+AGG - chr3_2:66428751-66428770 None:intergenic 50.0%
! GATGTGGCACAAGCCTTTTG+CGG + chr3_2:66428751-66428770 Msa0418640:three_prime_UTR 50.0%
TTGGGGGCGGTAGGTTTATC+GGG - chr3_2:66428517-66428536 None:intergenic 55.0%
GTTGGGGGCGGTAGGTTTAT+CGG - chr3_2:66428518-66428537 None:intergenic 55.0%
! TCGGCTAGTATTGAGGAGGC+TGG + chr3_2:66428575-66428594 Msa0418640:CDS 55.0%
ATTGAGGAGGCTGGAAGTGG+AGG + chr3_2:66428584-66428603 Msa0418640:CDS 55.0%
TTGGGGGCGGTAGGTTTATC+GGG - chr3_2:66428517-66428536 None:intergenic 55.0%
GTTGGGGGCGGTAGGTTTAT+CGG - chr3_2:66428518-66428537 None:intergenic 55.0%
! TCGGCTAGTATTGAGGAGGC+TGG + chr3_2:66428575-66428594 Msa0418640:CDS 55.0%
ATTGAGGAGGCTGGAAGTGG+AGG + chr3_2:66428584-66428603 Msa0418640:CDS 55.0%
GGGGCGGTAGGTTTATCGGG+TGG - chr3_2:66428514-66428533 None:intergenic 65.0%
CAGGCATAGTTGGGGGCGGT+AGG - chr3_2:66428526-66428545 None:intergenic 65.0%
CTACCGCCCCCAACTATGCC+TGG + chr3_2:66428524-66428543 Msa0418640:CDS 65.0%
CGTCCAGGCATAGTTGGGGG+CGG - chr3_2:66428530-66428549 None:intergenic 65.0%
GGGGCGGTAGGTTTATCGGG+TGG - chr3_2:66428514-66428533 None:intergenic 65.0%
CAGGCATAGTTGGGGGCGGT+AGG - chr3_2:66428526-66428545 None:intergenic 65.0%
CTACCGCCCCCAACTATGCC+TGG + chr3_2:66428524-66428543 Msa0418640:CDS 65.0%
CGTCCAGGCATAGTTGGGGG+CGG - chr3_2:66428530-66428549 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 66428085 66429064 66428085 ID=Msa0418640;Name=Msa0418640
chr3_2 mRNA 66428085 66429064 66428085 ID=Msa0418640-mRNA-1;Parent=Msa0418640;Name=Msa0418640-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|1|1|1|1|2|433|152
chr3_2 exon 66428085 66428354 66428085 ID=Msa0418640-mRNA-1:exon:15174;Parent=Msa0418640-mRNA-1
chr3_2 exon 66428443 66429064 66428443 ID=Msa0418640-mRNA-1:exon:15175;Parent=Msa0418640-mRNA-1
chr3_2 CDS 66428085 66428354 66428085 ID=Msa0418640-mRNA-1:cds;Parent=Msa0418640-mRNA-1
chr3_2 CDS 66428443 66428631 66428443 ID=Msa0418640-mRNA-1:cds;Parent=Msa0418640-mRNA-1
chr3_2 three_prime_UTR 66428632 66429064 66428632 ID=Msa0418640-mRNA-1:three_prime_utr;Parent=Msa0418640-mRNA-1
Gene Sequence

>Msa0418640

GCACCTTTCTATTTTTCTGAGAGGAGTATGCATCCACCTTCACCATATGCCGTGAACAATCCAGCTATGGAAACGAATGCTTATATACACCGTCATAAGCATACACCAGTTGAAGAGTTTCCAGAAAGACCTGGTGAACCTGAATGCAGTTTCTTCTTAAAAACCGGGGATTGCAAATTTAAATCCCATTGCAAATTTCATCATCCAAAGAACCGGATTACAAAATTACCTCCATGCAACCTAAGTGACAAGGGTCTGCCTTTGAGACCTGGCCAGAATGTGTGCACTCATTACAACCGATACGGAATTTGCAAATTTGGACCAGCTTGTAAGTATGACCACCCGATAAACCTACCGCCCCCAACTATGCCTGGACGTTATCAACAATCCTCTCACACAAACTCGGCTAGTATTGAGGAGGCTGGAAGTGGAGGAGCTAGTGATGCAACAATTTTATAG
Protein sequence

>Msa0418640

APFYFSERSMHPPSPYAVNNPAMETNAYIHRHKHTPVEEFPERPGEPECSFFLKTGDCKFKSHCKFHHPKNRITKLPPCNLSDKGLPLRPGQNVCTHYNRYGICKFGPACKYDHPINLPPPTMPGRYQQSSHTNSASIEEAGSGGASDATIL*