Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0181790 | Msa0425620 | 0.823095 | 1.665402e-53 | -8.615850e-47 |
| Msa0376960 | Msa0425620 | 0.808743 | 2.666148e-50 | -8.615850e-47 |
| Msa0425620 | Msa1373060 | 0.808859 | 2.517617e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATTATTTGTATCTTATT+TGG | 0.120705 | 3_2:+75986697 | Msa0425620:three_prime_UTR |
| GATGTAGTTATTTGCAAGTT+TGG | 0.186562 | 3_2:-75986095 | None:intergenic |
| ATATATACCATGTTTGAAAT+TGG | 0.194861 | 3_2:-75986241 | None:intergenic |
| TTATGAATTCTTTCAAATTA+AGG | 0.214260 | 3_2:+75986667 | Msa0425620:three_prime_UTR |
| AGCAACAAATGACTTCAATT+TGG | 0.266219 | 3_2:-75986163 | None:intergenic |
| CAATATTCTGCACCCTCTTA+TGG | 0.271660 | 3_2:+75986518 | Msa0425620:CDS |
| GAAGTCATTTGTTGCTCTCT+TGG | 0.321512 | 3_2:+75986170 | Msa0425620:CDS |
| ATTGTACTATTAGAATAAAT+TGG | 0.330696 | 3_2:+75986721 | Msa0425620:three_prime_UTR |
| CCCTCTTATGGTTCATTCCC+TGG | 0.350929 | 3_2:+75986530 | Msa0425620:CDS |
| ATGTAGTTATTTGCAAGTTT+GGG | 0.355745 | 3_2:-75986094 | None:intergenic |
| ACTACTCATGTCATCTATCA+TGG | 0.395752 | 3_2:+75986203 | Msa0425620:CDS |
| TACCATGTTTGAAATTGGAT+TGG | 0.399589 | 3_2:-75986236 | None:intergenic |
| AATATTATCACTTGAAGAAT+AGG | 0.400683 | 3_2:-75986066 | None:intergenic |
| CAGTTTAGTCACAGAGCAGC+TGG | 0.401012 | 3_2:-75986615 | None:intergenic |
| CAGCTGCTCTGTGACTAAAC+TGG | 0.421574 | 3_2:+75986616 | Msa0425620:three_prime_UTR |
| ACCAGGGAATGAACCATAAG+AGG | 0.435500 | 3_2:-75986531 | None:intergenic |
| GGTTCATTCCCTGGTGGTGG+AGG | 0.445646 | 3_2:+75986539 | Msa0425620:CDS |
| TATGGTTCATTCCCTGGTGG+TGG | 0.455953 | 3_2:+75986536 | Msa0425620:CDS |
| ATATATTGAGTACTAATTAA+CGG | 0.460298 | 3_2:-75986641 | None:intergenic |
| ACCATGTTTGAAATTGGATT+GGG | 0.461990 | 3_2:-75986235 | None:intergenic |
| AAGAGGGTGCAGAATATTGT+TGG | 0.468164 | 3_2:-75986514 | None:intergenic |
| CACATTAATAGCCCCACGGT+GGG | 0.549490 | 3_2:+75986576 | Msa0425620:exon |
| TATTTATATCCTCCACCACC+AGG | 0.549536 | 3_2:-75986548 | None:intergenic |
| CCACCGTGGGGCTATTAATG+TGG | 0.550176 | 3_2:-75986575 | None:intergenic |
| CTACTCATGTCATCTATCAT+GGG | 0.563545 | 3_2:+75986204 | Msa0425620:CDS |
| AATGACTTCAATTTGGAGAG+AGG | 0.591876 | 3_2:-75986156 | None:intergenic |
| AGTTTAGTCACAGAGCAGCT+GGG | 0.596374 | 3_2:-75986614 | None:intergenic |
| GCCCAATCCAATTTCAAACA+TGG | 0.609489 | 3_2:+75986234 | Msa0425620:CDS |
| CCGTGGGGCTATTAATGTGG+AGG | 0.630515 | 3_2:-75986572 | None:intergenic |
| TCTTATGGTTCATTCCCTGG+TGG | 0.633770 | 3_2:+75986533 | Msa0425620:CDS |
| CCAGGGAATGAACCATAAGA+GGG | 0.637077 | 3_2:-75986530 | None:intergenic |
| ACATTAATAGCCCCACGGTG+GGG | 0.640090 | 3_2:+75986577 | Msa0425620:exon |
| TTATCACTTGAAGAATAGGT+AGG | 0.640251 | 3_2:-75986062 | None:intergenic |
| CCACATTAATAGCCCCACGG+TGG | 0.681173 | 3_2:+75986575 | Msa0425620:exon |
| CCTCCACATTAATAGCCCCA+CGG | 0.685288 | 3_2:+75986572 | Msa0425620:exon |
| ATTTATATCCTCCACCACCA+GGG | 0.692132 | 3_2:-75986547 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTATTTTTATAAGCAA+TGG | - | chr3_2:75986785-75986804 | None:intergenic | 10.0% |
| !!! | ATTATTATTTTTATAAGCAA+TGG | - | chr3_2:75986785-75986804 | None:intergenic | 10.0% |
| !!! | ATAGTATATATTTTGAATCA+GGG | - | chr3_2:75986323-75986342 | None:intergenic | 15.0% |
| !!! | TATAGTATATATTTTGAATC+AGG | - | chr3_2:75986324-75986343 | None:intergenic | 15.0% |
| !!! | TCACTTATCTTTTTTATATA+TGG | + | chr3_2:75986473-75986492 | Msa0425620:intron | 15.0% |
| !! | ATATATTGAGTACTAATTAA+CGG | - | chr3_2:75986644-75986663 | None:intergenic | 15.0% |
| !! | TTATGAATTCTTTCAAATTA+AGG | + | chr3_2:75986667-75986686 | Msa0425620:three_prime_UTR | 15.0% |
| !! | CAAATTATTTGTATCTTATT+TGG | + | chr3_2:75986697-75986716 | Msa0425620:three_prime_UTR | 15.0% |
| !! | ATTGTACTATTAGAATAAAT+TGG | + | chr3_2:75986721-75986740 | Msa0425620:three_prime_UTR | 15.0% |
| !!! | ATAGTATATATTTTGAATCA+GGG | - | chr3_2:75986323-75986342 | None:intergenic | 15.0% |
| !!! | TATAGTATATATTTTGAATC+AGG | - | chr3_2:75986324-75986343 | None:intergenic | 15.0% |
| !!! | TCACTTATCTTTTTTATATA+TGG | + | chr3_2:75986473-75986492 | Msa0425620:intron | 15.0% |
| !! | ATATATTGAGTACTAATTAA+CGG | - | chr3_2:75986644-75986663 | None:intergenic | 15.0% |
| !! | TTATGAATTCTTTCAAATTA+AGG | + | chr3_2:75986667-75986686 | Msa0425620:three_prime_UTR | 15.0% |
| !! | CAAATTATTTGTATCTTATT+TGG | + | chr3_2:75986697-75986716 | Msa0425620:three_prime_UTR | 15.0% |
| !! | ATTGTACTATTAGAATAAAT+TGG | + | chr3_2:75986721-75986740 | Msa0425620:three_prime_UTR | 15.0% |
| !! | AATATTATCACTTGAAGAAT+AGG | - | chr3_2:75986069-75986088 | None:intergenic | 20.0% |
| !!! | ATATATACCATGTTTGAAAT+TGG | - | chr3_2:75986244-75986263 | None:intergenic | 20.0% |
| !! | AATATTATCACTTGAAGAAT+AGG | - | chr3_2:75986069-75986088 | None:intergenic | 20.0% |
| !!! | ATATATACCATGTTTGAAAT+TGG | - | chr3_2:75986244-75986263 | None:intergenic | 20.0% |
| ! | ATGTAGTTATTTGCAAGTTT+GGG | - | chr3_2:75986097-75986116 | None:intergenic | 25.0% |
| ! | ATAGTAAGAATCATATCACA+TGG | + | chr3_2:75986291-75986310 | Msa0425620:intron | 25.0% |
| ! | TATAATCATATGCTTATTCG+AGG | + | chr3_2:75986340-75986359 | Msa0425620:intron | 25.0% |
| ! | CATTTAACCAATTGGAATTT+GGG | + | chr3_2:75986366-75986385 | Msa0425620:intron | 25.0% |
| !! | AATTTGGGATTGATTATTGA+TGG | + | chr3_2:75986381-75986400 | Msa0425620:intron | 25.0% |
| ! | ATGTAGTTATTTGCAAGTTT+GGG | - | chr3_2:75986097-75986116 | None:intergenic | 25.0% |
| ! | ATAGTAAGAATCATATCACA+TGG | + | chr3_2:75986291-75986310 | Msa0425620:intron | 25.0% |
| ! | TATAATCATATGCTTATTCG+AGG | + | chr3_2:75986340-75986359 | Msa0425620:intron | 25.0% |
| ! | CATTTAACCAATTGGAATTT+GGG | + | chr3_2:75986366-75986385 | Msa0425620:intron | 25.0% |
| !! | AATTTGGGATTGATTATTGA+TGG | + | chr3_2:75986381-75986400 | Msa0425620:intron | 25.0% |
| TTATCACTTGAAGAATAGGT+AGG | - | chr3_2:75986065-75986084 | None:intergenic | 30.0% | |
| GATGTAGTTATTTGCAAGTT+TGG | - | chr3_2:75986098-75986117 | None:intergenic | 30.0% | |
| AGCAACAAATGACTTCAATT+TGG | - | chr3_2:75986166-75986185 | None:intergenic | 30.0% | |
| ! | ACCATGTTTGAAATTGGATT+GGG | - | chr3_2:75986238-75986257 | None:intergenic | 30.0% |
| ! | TACCATGTTTGAAATTGGAT+TGG | - | chr3_2:75986239-75986258 | None:intergenic | 30.0% |
| CCAAATTCCAATTGGTTAAA+TGG | - | chr3_2:75986368-75986387 | None:intergenic | 30.0% | |
| CCATTTAACCAATTGGAATT+TGG | + | chr3_2:75986365-75986384 | Msa0425620:intron | 30.0% | |
| AATCAATCCCAAATTCCAAT+TGG | - | chr3_2:75986376-75986395 | None:intergenic | 30.0% | |
| !!! | ATCTTTTTTATATATGGCGC+AGG | + | chr3_2:75986479-75986498 | Msa0425620:intron | 30.0% |
| !!! | TCTTTTTTATATATGGCGCA+GGG | + | chr3_2:75986480-75986499 | Msa0425620:intron | 30.0% |
| TTATCACTTGAAGAATAGGT+AGG | - | chr3_2:75986065-75986084 | None:intergenic | 30.0% | |
| GATGTAGTTATTTGCAAGTT+TGG | - | chr3_2:75986098-75986117 | None:intergenic | 30.0% | |
| AGCAACAAATGACTTCAATT+TGG | - | chr3_2:75986166-75986185 | None:intergenic | 30.0% | |
| ! | ACCATGTTTGAAATTGGATT+GGG | - | chr3_2:75986238-75986257 | None:intergenic | 30.0% |
| ! | TACCATGTTTGAAATTGGAT+TGG | - | chr3_2:75986239-75986258 | None:intergenic | 30.0% |
| CCAAATTCCAATTGGTTAAA+TGG | - | chr3_2:75986368-75986387 | None:intergenic | 30.0% | |
| CCATTTAACCAATTGGAATT+TGG | + | chr3_2:75986365-75986384 | Msa0425620:intron | 30.0% | |
| AATCAATCCCAAATTCCAAT+TGG | - | chr3_2:75986376-75986395 | None:intergenic | 30.0% | |
| !!! | ATCTTTTTTATATATGGCGC+AGG | + | chr3_2:75986479-75986498 | Msa0425620:intron | 30.0% |
| !!! | TCTTTTTTATATATGGCGCA+GGG | + | chr3_2:75986480-75986499 | Msa0425620:intron | 30.0% |
| AATGACTTCAATTTGGAGAG+AGG | - | chr3_2:75986159-75986178 | None:intergenic | 35.0% | |
| ACTACTCATGTCATCTATCA+TGG | + | chr3_2:75986203-75986222 | Msa0425620:CDS | 35.0% | |
| CTACTCATGTCATCTATCAT+GGG | + | chr3_2:75986204-75986223 | Msa0425620:CDS | 35.0% | |
| AATGACTTCAATTTGGAGAG+AGG | - | chr3_2:75986159-75986178 | None:intergenic | 35.0% | |
| ACTACTCATGTCATCTATCA+TGG | + | chr3_2:75986203-75986222 | Msa0425620:CDS | 35.0% | |
| CTACTCATGTCATCTATCAT+GGG | + | chr3_2:75986204-75986223 | Msa0425620:CDS | 35.0% | |
| ! | GAAGTCATTTGTTGCTCTCT+TGG | + | chr3_2:75986170-75986189 | Msa0425620:CDS | 40.0% |
| GCCCAATCCAATTTCAAACA+TGG | + | chr3_2:75986234-75986253 | Msa0425620:CDS | 40.0% | |
| ! | AAGAGGGTGCAGAATATTGT+TGG | - | chr3_2:75986517-75986536 | None:intergenic | 40.0% |
| CAATATTCTGCACCCTCTTA+TGG | + | chr3_2:75986518-75986537 | Msa0425620:CDS | 40.0% | |
| ATTTATATCCTCCACCACCA+GGG | - | chr3_2:75986550-75986569 | None:intergenic | 40.0% | |
| TATTTATATCCTCCACCACC+AGG | - | chr3_2:75986551-75986570 | None:intergenic | 40.0% | |
| ! | GAAGTCATTTGTTGCTCTCT+TGG | + | chr3_2:75986170-75986189 | Msa0425620:CDS | 40.0% |
| GCCCAATCCAATTTCAAACA+TGG | + | chr3_2:75986234-75986253 | Msa0425620:CDS | 40.0% | |
| ! | AAGAGGGTGCAGAATATTGT+TGG | - | chr3_2:75986517-75986536 | None:intergenic | 40.0% |
| CAATATTCTGCACCCTCTTA+TGG | + | chr3_2:75986518-75986537 | Msa0425620:CDS | 40.0% | |
| ATTTATATCCTCCACCACCA+GGG | - | chr3_2:75986550-75986569 | None:intergenic | 40.0% | |
| TATTTATATCCTCCACCACC+AGG | - | chr3_2:75986551-75986570 | None:intergenic | 40.0% | |
| CGAGGCTCCATTTAACCAAT+TGG | + | chr3_2:75986358-75986377 | Msa0425620:intron | 45.0% | |
| CCAGGGAATGAACCATAAGA+GGG | - | chr3_2:75986533-75986552 | None:intergenic | 45.0% | |
| ACCAGGGAATGAACCATAAG+AGG | - | chr3_2:75986534-75986553 | None:intergenic | 45.0% | |
| TCTTATGGTTCATTCCCTGG+TGG | + | chr3_2:75986533-75986552 | Msa0425620:CDS | 45.0% | |
| ! | TGATTATTTTCCCCACCGTG+GGG | - | chr3_2:75986590-75986609 | None:intergenic | 45.0% |
| ! | GTGATTATTTTCCCCACCGT+GGG | - | chr3_2:75986591-75986610 | None:intergenic | 45.0% |
| ! | AGTGATTATTTTCCCCACCG+TGG | - | chr3_2:75986592-75986611 | None:intergenic | 45.0% |
| AGTTTAGTCACAGAGCAGCT+GGG | - | chr3_2:75986617-75986636 | None:intergenic | 45.0% | |
| CGAGGCTCCATTTAACCAAT+TGG | + | chr3_2:75986358-75986377 | Msa0425620:intron | 45.0% | |
| CCAGGGAATGAACCATAAGA+GGG | - | chr3_2:75986533-75986552 | None:intergenic | 45.0% | |
| ACCAGGGAATGAACCATAAG+AGG | - | chr3_2:75986534-75986553 | None:intergenic | 45.0% | |
| TCTTATGGTTCATTCCCTGG+TGG | + | chr3_2:75986533-75986552 | Msa0425620:CDS | 45.0% | |
| ! | TGATTATTTTCCCCACCGTG+GGG | - | chr3_2:75986590-75986609 | None:intergenic | 45.0% |
| ! | GTGATTATTTTCCCCACCGT+GGG | - | chr3_2:75986591-75986610 | None:intergenic | 45.0% |
| ! | AGTGATTATTTTCCCCACCG+TGG | - | chr3_2:75986592-75986611 | None:intergenic | 45.0% |
| AGTTTAGTCACAGAGCAGCT+GGG | - | chr3_2:75986617-75986636 | None:intergenic | 45.0% | |
| CCCTCTTATGGTTCATTCCC+TGG | + | chr3_2:75986530-75986549 | Msa0425620:CDS | 50.0% | |
| TATGGTTCATTCCCTGGTGG+TGG | + | chr3_2:75986536-75986555 | Msa0425620:CDS | 50.0% | |
| CCTCCACATTAATAGCCCCA+CGG | + | chr3_2:75986572-75986591 | Msa0425620:exon | 50.0% | |
| CACATTAATAGCCCCACGGT+GGG | + | chr3_2:75986576-75986595 | Msa0425620:exon | 50.0% | |
| ACATTAATAGCCCCACGGTG+GGG | + | chr3_2:75986577-75986596 | Msa0425620:exon | 50.0% | |
| CAGTTTAGTCACAGAGCAGC+TGG | - | chr3_2:75986618-75986637 | None:intergenic | 50.0% | |
| CAGCTGCTCTGTGACTAAAC+TGG | + | chr3_2:75986616-75986635 | Msa0425620:three_prime_UTR | 50.0% | |
| CCCTCTTATGGTTCATTCCC+TGG | + | chr3_2:75986530-75986549 | Msa0425620:CDS | 50.0% | |
| TATGGTTCATTCCCTGGTGG+TGG | + | chr3_2:75986536-75986555 | Msa0425620:CDS | 50.0% | |
| CCTCCACATTAATAGCCCCA+CGG | + | chr3_2:75986572-75986591 | Msa0425620:exon | 50.0% | |
| CACATTAATAGCCCCACGGT+GGG | + | chr3_2:75986576-75986595 | Msa0425620:exon | 50.0% | |
| ACATTAATAGCCCCACGGTG+GGG | + | chr3_2:75986577-75986596 | Msa0425620:exon | 50.0% | |
| CAGTTTAGTCACAGAGCAGC+TGG | - | chr3_2:75986618-75986637 | None:intergenic | 50.0% | |
| CAGCTGCTCTGTGACTAAAC+TGG | + | chr3_2:75986616-75986635 | Msa0425620:three_prime_UTR | 50.0% | |
| CCGTGGGGCTATTAATGTGG+AGG | - | chr3_2:75986575-75986594 | None:intergenic | 55.0% | |
| CCACCGTGGGGCTATTAATG+TGG | - | chr3_2:75986578-75986597 | None:intergenic | 55.0% | |
| CCACATTAATAGCCCCACGG+TGG | + | chr3_2:75986575-75986594 | Msa0425620:exon | 55.0% | |
| CCGTGGGGCTATTAATGTGG+AGG | - | chr3_2:75986575-75986594 | None:intergenic | 55.0% | |
| CCACCGTGGGGCTATTAATG+TGG | - | chr3_2:75986578-75986597 | None:intergenic | 55.0% | |
| CCACATTAATAGCCCCACGG+TGG | + | chr3_2:75986575-75986594 | Msa0425620:exon | 55.0% | |
| !! | GGTTCATTCCCTGGTGGTGG+AGG | + | chr3_2:75986539-75986558 | Msa0425620:CDS | 60.0% |
| !! | GGTTCATTCCCTGGTGGTGG+AGG | + | chr3_2:75986539-75986558 | Msa0425620:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 75986037 | 75986837 | 75986037 | ID=Msa0425620;Name=Msa0425620 |
| chr3_2 | mRNA | 75986037 | 75986837 | 75986037 | ID=Msa0425620-mRNA-1;Parent=Msa0425620;Name=Msa0425620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=113|1|1|1|1|1|2|254|62 |
| chr3_2 | exon | 75986037 | 75986255 | 75986037 | ID=Msa0425620-mRNA-1:exon:19522;Parent=Msa0425620-mRNA-1 |
| chr3_2 | exon | 75986501 | 75986837 | 75986501 | ID=Msa0425620-mRNA-1:exon:19523;Parent=Msa0425620-mRNA-1 |
| chr3_2 | five_prime_UTR | 75986037 | 75986149 | 75986037 | ID=Msa0425620-mRNA-1:five_prime_utr;Parent=Msa0425620-mRNA-1 |
| chr3_2 | CDS | 75986150 | 75986255 | 75986150 | ID=Msa0425620-mRNA-1:cds;Parent=Msa0425620-mRNA-1 |
| chr3_2 | CDS | 75986501 | 75986583 | 75986501 | ID=Msa0425620-mRNA-1:cds;Parent=Msa0425620-mRNA-1 |
| chr3_2 | three_prime_UTR | 75986584 | 75986837 | 75986584 | ID=Msa0425620-mRNA-1:three_prime_utr;Parent=Msa0425620-mRNA-1 |
| Gene Sequence |
| Protein sequence |
