AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0428220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0428220 MtrunA17_Chr3g0131441 98.521 541 8 0 1 541 1 541 0.0 1077
Msa0428220 MtrunA17_Chr8g0385501 77.273 550 106 6 1 541 1 540 0.0 795
Msa0428220 MtrunA17_Chr4g0033731 33.995 403 198 13 8 402 8 350 1.05e-58 201
Msa0428220 MtrunA17_Chr1g0192661 38.421 380 175 11 33 405 26 353 2.86e-58 199
Msa0428220 MtrunA17_Chr7g0249551 34.230 409 196 14 1 398 1 347 1.01e-56 195
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0428220 AT5G61790.1 74.953 531 126 3 11 541 7 530 0.0 801
Msa0428220 AT5G07340.1 75.147 511 117 3 31 539 27 529 0.0 771
Msa0428220 AT5G07340.2 75.394 508 115 3 34 539 38 537 0.0 769
Msa0428220 AT1G56340.2 38.702 416 189 14 5 413 3 359 1.23e-61 209
Msa0428220 AT1G56340.1 38.702 416 189 14 5 413 3 359 3.06e-61 208
Msa0428220 AT1G09210.1 38.861 404 183 13 9 405 5 351 8.04e-59 201
Msa0428220 AT1G08450.1 33.333 429 217 13 11 423 8 383 1.28e-56 196
Msa0428220 AT1G08450.3 30.023 433 201 13 11 423 8 358 4.94e-43 158
Msa0428220 AT1G08450.2 34.454 238 106 6 194 423 134 329 1.93e-25 108

Find 211 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTGGCTTTACAGCTTC+TGG 0.065396 3_2:+79062235 None:intergenic
TTACAATAATCTTATCTTTC+TGG 0.129339 3_2:+79061524 None:intergenic
TCATCATGAGTGGTTAATTT+TGG 0.134496 3_2:-79061010 Msa0428220:three_prime_UTR
CTGCCATTGGTATTGAAATT+TGG 0.163969 3_2:-79061865 Msa0428220:CDS
CATTGATAACCCAGCTTATA+AGG 0.184554 3_2:-79061960 Msa0428220:CDS
AAATATATCTCTTTCCTTTC+TGG 0.190272 3_2:-79060747 Msa0428220:three_prime_UTR
AGTGAGGGGCACGATGATTT+TGG 0.222943 3_2:-79062784 Msa0428220:CDS
AGTATATAATCTTTCGAAAA+AGG 0.224841 3_2:+79060938 None:intergenic
CCACTCATGATGATACTTAA+TGG 0.227766 3_2:+79061020 None:intergenic
AGAAAGGAAAGAGATATATT+TGG 0.240188 3_2:+79060749 None:intergenic
GAAAAGGAACCCAGCTTATA+AGG 0.242385 3_2:-79062002 Msa0428220:CDS
TCAACATACTTTCCAGTCTT+TGG 0.247163 3_2:+79062490 None:intergenic
TGGGTTTCCAACCACTCTCC+TGG 0.263681 3_2:+79062608 None:intergenic
CTCTTTCTCAATGTTAAACT+TGG 0.276239 3_2:+79061754 None:intergenic
TTCAAAGAGAGAGTGATAAA+TGG 0.284592 3_2:+79064105 None:intergenic
GCTCTCTCGTCCCAGTCTTC+TGG 0.293148 3_2:+79062271 None:intergenic
GAGATATATTTGGGAACATT+AGG 0.299898 3_2:+79060759 None:intergenic
TTCGTCAAGTTCTTTAACTA+TGG 0.306137 3_2:+79062729 None:intergenic
TTCGAAAGATTATATACTTA+AGG 0.316968 3_2:-79060933 Msa0428220:three_prime_UTR
AGAATTCTCACCTCATTATC+AGG 0.319935 3_2:+79062397 None:intergenic
TTTGTTGCCCCGCACCTGTC+TGG 0.320390 3_2:+79062544 None:intergenic
CTCCACTTTCCCTTATAAGC+TGG 0.323256 3_2:+79061992 None:intergenic
ACCCAGCTTATAAGGGTATA+TGG 0.329615 3_2:-79061952 Msa0428220:CDS
AGCTTCTGGGTCAGGAATCT+TGG 0.330240 3_2:+79062249 None:intergenic
AGAATTCCAATAGTCAGATT+TGG 0.331821 3_2:+79061369 None:intergenic
GCTAGAGACAGGCCCGTATT+TGG 0.332467 3_2:+79060805 None:intergenic
CAGGGGCTGATTCACACTTT+GGG 0.332470 3_2:+79062050 None:intergenic
GGCTTTACAGCTTCTGGGTC+AGG 0.336142 3_2:+79062241 None:intergenic
TTCATTCTCTCCACCAGCTT+GGG 0.340196 3_2:+79061131 None:intergenic
TCCTTTCAATTGTCTCGCTT+TGG 0.350249 3_2:+79061063 None:intergenic
CCTCTGGCTTTACAGCTTCT+GGG 0.362016 3_2:+79062236 None:intergenic
ACTATGGCGTACTTCCTTGC+TGG 0.364602 3_2:+79062745 None:intergenic
AGTTCAAAGTACTCTGGGTT+TGG 0.371725 3_2:+79061914 None:intergenic
GGTGGATTTAAGTTTGGTAA+TGG 0.373109 3_2:-79060699 Msa0428220:three_prime_UTR
GGACCAAACATGACAGAATA+TGG 0.377257 3_2:+79062565 None:intergenic
CTCTTCCACTCACCACAGCC+AGG 0.377474 3_2:+79062031 None:intergenic
TCTTCTGGTTTGTTATCATC+AGG 0.380136 3_2:+79062286 None:intergenic
CCAGGGGCTGATTCACACTT+TGG 0.380770 3_2:+79062049 None:intergenic
AAGCAACCAAATCTGACTAT+TGG 0.383408 3_2:-79061375 Msa0428220:CDS
GGGTTTCCAACCACTCTCCT+GGG 0.383983 3_2:+79062609 None:intergenic
GAAAGGAAAGAGATATATTT+GGG 0.385452 3_2:+79060750 None:intergenic
CTTCATTCTCTCCACCAGCT+TGG 0.390458 3_2:+79061130 None:intergenic
CACTCATGATGATACTTAAT+GGG 0.390619 3_2:+79061021 None:intergenic
TCTCCATATTCTGTCATGTT+TGG 0.391928 3_2:-79062568 Msa0428220:CDS
TGACGAACCTGTCATTCTTA+AGG 0.393509 3_2:-79062713 Msa0428220:CDS
TCGTCATCCAACCATCCTTC+AGG 0.394777 3_2:+79062163 None:intergenic
GGTTTGTTATCATCAGGATC+AGG 0.396865 3_2:+79062292 None:intergenic
GCTGAGTCCGAGGGTATTGC+TGG 0.398364 3_2:-79061704 Msa0428220:CDS
TTCTCAAGTTCAAAGTACTC+TGG 0.413427 3_2:+79061908 None:intergenic
TTCCATATACCCTTATAAGC+TGG 0.413553 3_2:+79061950 None:intergenic
GGCAACAAAACCTGAGGATT+GGG 0.417078 3_2:-79062116 Msa0428220:CDS
TCCACTTTCCCTTATAAGCT+GGG 0.417460 3_2:+79061993 None:intergenic
CCAACGTGGCTCAAAACAAT+TGG 0.417883 3_2:-79064195 Msa0428220:five_prime_UTR
GCAATATGATTGGTTGAAAC+AGG 0.418120 3_2:-79064155 Msa0428220:five_prime_UTR
AGCTGTAAAGCCAGAGGACT+GGG 0.421453 3_2:-79062230 Msa0428220:CDS
CTGGCTGTGGTGAGTGGAAG+AGG 0.424277 3_2:-79062030 Msa0428220:CDS
TCAATACCAATGGCAGCAAT+AGG 0.424596 3_2:+79061872 None:intergenic
TGACGGCGAGGAGAAGAAAA+AGG 0.425936 3_2:-79062371 Msa0428220:CDS
TGATGAGGAGGATGGTGAAT+GGG 0.428154 3_2:-79062092 Msa0428220:CDS
ACAAGGTGCACTTCATCTTC+AGG 0.433871 3_2:-79062522 Msa0428220:CDS
TGTTTGGTCCAGACAGGTGC+GGG 0.435806 3_2:-79062552 Msa0428220:CDS
TCTTATCTTTCTGGCCACTT+AGG 0.437920 3_2:+79061533 None:intergenic
CTCATTATCAGGCTTTAGAA+TGG 0.437946 3_2:+79062408 None:intergenic
TACCGCATCGTCAGCTTCAT+CGG 0.439150 3_2:+79063905 None:intergenic
TCTGCAGGAAATCATTGAAA+AGG 0.446463 3_2:-79061403 Msa0428220:intron
ATAATTGTACAGCTACAATT+AGG 0.447220 3_2:+79060873 None:intergenic
ATGATGAGGAGGATGGTGAA+TGG 0.451613 3_2:-79062093 Msa0428220:CDS
CTGTCATGTTTGGTCCAGAC+AGG 0.452777 3_2:-79062558 Msa0428220:CDS
CTGCAGGAAATCATTGAAAA+GGG 0.453156 3_2:-79061402 Msa0428220:intron
TTTGAGCCTATTGCTGCCAT+TGG 0.460151 3_2:-79061878 Msa0428220:CDS
ATCTTTCTGGCCACTTAGGA+AGG 0.460977 3_2:+79061537 None:intergenic
TCCATATACCCTTATAAGCT+GGG 0.462245 3_2:+79061951 None:intergenic
GAATGAAGACAACAAAGAAA+AGG 0.463345 3_2:-79061115 Msa0428220:CDS
CTGGGATGAGGATGCACCTA+TGG 0.465153 3_2:-79062212 Msa0428220:CDS
AGTTTCTGTGCGTTCTGTGT+TGG 0.466104 3_2:+79061155 None:intergenic
TCTCAAGTTCAAAGTACTCT+GGG 0.467035 3_2:+79061909 None:intergenic
AAAAGGAACCCAGCTTATAA+GGG 0.467436 3_2:-79062001 Msa0428220:CDS
ATAAGCTGGGTTATCAATGT+AGG 0.471071 3_2:+79061964 None:intergenic
TCTGGGTCATCTATTTCCTC+TGG 0.474079 3_2:+79062139 None:intergenic
AAGAGGAAGCAATGAAGAAA+AGG 0.475362 3_2:+79063943 None:intergenic
TCTTCCACTCACCACAGCCA+GGG 0.476497 3_2:+79062032 None:intergenic
GCATCCTCATCCCAGTCCTC+TGG 0.476556 3_2:+79062220 None:intergenic
TCAAAGAGAGAGTGATAAAT+GGG 0.476872 3_2:+79064106 None:intergenic
TCCTCATCATCCCAATCCTC+AGG 0.477110 3_2:+79062106 None:intergenic
GATTCGTTGTCAAATCCCTT+GGG 0.478126 3_2:+79062589 None:intergenic
GAAAGGATTGAAACTTATAG+TGG 0.479137 3_2:-79061047 Msa0428220:three_prime_UTR
AGATTCGTTGTCAAATCCCT+TGG 0.479561 3_2:+79062588 None:intergenic
AGGCAACAAAACCTGAGGAT+TGG 0.480179 3_2:-79062117 Msa0428220:CDS
TCTTTCTCAATGTTAAACTT+GGG 0.480481 3_2:+79061755 None:intergenic
TGGCCTCCTACGTGGAGCAT+TGG 0.487308 3_2:+79061083 None:intergenic
AAGAAACAGTGGCTAGAGAC+AGG 0.488905 3_2:+79060794 None:intergenic
TATATGCACCACCACATTCA+AGG 0.490647 3_2:+79062647 None:intergenic
CACGTGGCACGCAATATGAT+TGG 0.490679 3_2:-79064165 Msa0428220:five_prime_UTR
AAGCTGTAAAGCCAGAGGAC+TGG 0.491241 3_2:-79062231 Msa0428220:CDS
ATTGATAACCCAGCTTATAA+GGG 0.496616 3_2:-79061959 Msa0428220:CDS
ATGATAACAAACCAGAAGAC+TGG 0.496997 3_2:-79062282 Msa0428220:CDS
CCGTTGGATTGTATCTGAAA+AGG 0.499726 3_2:-79063770 Msa0428220:CDS
CGAGGGTATTGCTGGCTACC+AGG 0.502715 3_2:-79061696 Msa0428220:intron
TATTGAAATTTGGACAATGC+AGG 0.503304 3_2:-79061855 Msa0428220:CDS
TAAGCTGGGTTATCAATGTA+GGG 0.504397 3_2:+79061965 None:intergenic
TCTTCTGCAGGCTAAGGTGG+AGG 0.505920 3_2:-79061196 Msa0428220:intron
AATCCTATCGTGAAACTACA+TGG 0.508933 3_2:-79061781 Msa0428220:CDS
CACAGAGACATTAGCAATGG+TGG 0.513766 3_2:-79064000 Msa0428220:exon
GCTCAAAATCCTCCTCGGAC+AGG 0.517116 3_2:+79062341 None:intergenic
ATAAGGGTATATGGAAGCCT+CGG 0.523060 3_2:-79061943 Msa0428220:CDS
AGGAGCACCGCGATAGCGAT+AGG 0.523693 3_2:+79063963 None:intergenic
CAGCCCCTGGCTGTGGTGAG+TGG 0.526056 3_2:-79062036 Msa0428220:CDS
TTTCCATGTAGTTTCACGAT+AGG 0.527990 3_2:+79061778 None:intergenic
TTCAACCCCAGGAGAGTGGT+TGG 0.532251 3_2:-79062615 Msa0428220:CDS
GGAGGCAGCAGCTGAGTCCG+AGG 0.532327 3_2:-79061714 Msa0428220:CDS
GTTTGTTATCATCAGGATCA+GGG 0.534077 3_2:+79062293 None:intergenic
AGAGAAACAGAAGGCTGAAG+AGG 0.535396 3_2:-79061735 Msa0428220:CDS
GATCCATCCTTAAGAATGAC+AGG 0.536585 3_2:+79062706 None:intergenic
ATCAGGATCAGGGATTGTCT+TGG 0.536695 3_2:+79062303 None:intergenic
TGTCCAAATTTCAATACCAA+TGG 0.537978 3_2:+79061862 None:intergenic
TAAGGGTATATGGAAGCCTC+GGG 0.538372 3_2:-79061942 Msa0428220:CDS
ACCCAGCTTATAAGGGAAAG+TGG 0.538755 3_2:-79061994 Msa0428220:CDS
CATTCTAAAGCCTGATAATG+AGG 0.539931 3_2:-79062407 Msa0428220:CDS
TCTTCATCAATAATTTCCAT+AGG 0.540356 3_2:+79062196 None:intergenic
ACCTGAGGATTGGGATGATG+AGG 0.540446 3_2:-79062107 Msa0428220:CDS
CTTCCACTCACCACAGCCAG+GGG 0.542397 3_2:+79062033 None:intergenic
TTTGAGACTCGCCTTCAGAA+TGG 0.542633 3_2:-79062670 Msa0428220:CDS
GCAGCAATAGGCTCAAAGTC+AGG 0.545429 3_2:+79061884 None:intergenic
CGCACAGAAACTTCCCAAGC+TGG 0.545555 3_2:-79061144 Msa0428220:CDS
GAAACAGAAGGCTGAAGAGG+AGG 0.547348 3_2:-79061732 Msa0428220:CDS
AGTTTGTCAGATGGGACAGA+AGG 0.547484 3_2:+79062448 None:intergenic
CATGAGATAGTTTGTCAGAT+GGG 0.548220 3_2:+79062440 None:intergenic
GTTTGTCAGATGGGACAGAA+GGG 0.550204 3_2:+79062449 None:intergenic
CCAAAGTGTGAATCAGCCCC+TGG 0.551044 3_2:-79062049 Msa0428220:CDS
TTGTCAGATGGGACAGAAGG+GGG 0.552914 3_2:+79062451 None:intergenic
GCAGGCTAAGGTGGAGGCGA+AGG 0.553851 3_2:-79061190 Msa0428220:intron
AGTGGAAGAGGCCAACGAAA+AGG 0.554259 3_2:-79062018 Msa0428220:CDS
GGTTTCCAACCACTCTCCTG+GGG 0.554990 3_2:+79062610 None:intergenic
ATTAGCAATGGTGGAACGAA+AGG 0.558203 3_2:-79063991 Msa0428220:exon
CACATTCAAGGCCATTCTGA+AGG 0.558745 3_2:+79062659 None:intergenic
CAGGTGCGGGGCAACAAACA+AGG 0.564763 3_2:-79062539 Msa0428220:CDS
AAAGCCAGAGGACTGGGATG+AGG 0.564770 3_2:-79062224 Msa0428220:CDS
AAACACTCGGAGTATCCAAA+AGG 0.566204 3_2:+79060898 None:intergenic
GGAGCACCGCGATAGCGATA+GGG 0.567430 3_2:+79063964 None:intergenic
TGATAATATTCTTATAGCGA+AGG 0.569068 3_2:-79061819 Msa0428220:CDS
ATGTTTGGTCCAGACAGGTG+CGG 0.569118 3_2:-79062553 Msa0428220:CDS
GGATCAGGGATTGTCTTGGA+AGG 0.571851 3_2:+79062307 None:intergenic
TCTTTCTGGCCACTTAGGAA+GGG 0.574034 3_2:+79061538 None:intergenic
GATTGGGATGATGAGGAGGA+TGG 0.574150 3_2:-79062100 Msa0428220:CDS
TGTGAATCAGCCCCTGGCTG+TGG 0.575974 3_2:-79062043 Msa0428220:CDS
GAGGTGAGAATTCTAGTTGA+CGG 0.579771 3_2:-79062388 Msa0428220:CDS
CAGCCAATGCTCCACGTAGG+AGG 0.580449 3_2:-79061086 Msa0428220:CDS
ATAGGCTAGATCGGCCAGAA+AGG 0.582929 3_2:+79060733 None:intergenic
TCTTTAGGCTCATATTCGGC+GGG 0.582978 3_2:-79061314 Msa0428220:CDS
TTCTTTAGGCTCATATTCGG+CGG 0.583806 3_2:-79061315 Msa0428220:CDS
TGGAAGCATTCTAAGAGTGA+GGG 0.585471 3_2:-79062799 Msa0428220:CDS
GGAAGCGTGAACGAAGGAAG+AGG 0.587228 3_2:+79063926 None:intergenic
CTGGAAGCATTCTAAGAGTG+AGG 0.587589 3_2:-79062800 Msa0428220:CDS
GGAGAGTGGTTGGAAACCCA+AGG 0.591216 3_2:-79062605 Msa0428220:CDS
GCAGACATTCCCTTCCTAAG+TGG 0.593740 3_2:-79061547 Msa0428220:CDS
GAACCTGTCATTCTTAAGGA+TGG 0.594415 3_2:-79062709 Msa0428220:CDS
AGAAGGCTCAAAATCCTCCT+CGG 0.597536 3_2:+79062336 None:intergenic
TATTAAATATCTTCAACCCC+AGG 0.601083 3_2:-79062626 Msa0428220:CDS
TGAGAAACCTGAAGGATGGT+TGG 0.601270 3_2:-79062170 Msa0428220:CDS
TCTGGGTTTGGAATCTCCCG+AGG 0.605370 3_2:+79061926 None:intergenic
ACATGAGATAGTTTGTCAGA+TGG 0.606072 3_2:+79062439 None:intergenic
TCTCGCTTTGGCCTCCTACG+TGG 0.607453 3_2:+79061075 None:intergenic
GTCTTGGAAGGGATAAGAGA+AGG 0.612453 3_2:+79062319 None:intergenic
GAGGCAGCAGCTGAGTCCGA+GGG 0.612757 3_2:-79061713 Msa0428220:CDS
CTTCAGAATGGCCTTGAATG+TGG 0.614341 3_2:-79062658 Msa0428220:CDS
TATCTTCAACCCCAGGAGAG+TGG 0.614389 3_2:-79062619 Msa0428220:CDS
TTTGTCAGATGGGACAGAAG+GGG 0.617670 3_2:+79062450 None:intergenic
TGGAATTCTTGCATCCGTTG+TGG 0.618008 3_2:-79061355 Msa0428220:CDS
TGTCTGCGATCTTGTACAAG+AGG 0.619339 3_2:+79061563 None:intergenic
TTCTTGTTAGTGAACCAGCA+AGG 0.619414 3_2:-79062759 Msa0428220:CDS
GAGCACCGCGATAGCGATAG+GGG 0.620301 3_2:+79063965 None:intergenic
AAGCTGAGAAACCTGAAGGA+TGG 0.623492 3_2:-79062174 Msa0428220:CDS
AACAATTGGTTTAAGTCACG+TGG 0.623758 3_2:-79064181 Msa0428220:five_prime_UTR
TGCAGGAAATCATTGAAAAG+GGG 0.624295 3_2:-79061401 Msa0428220:intron
CAGAATGGCCTTGAATGTGG+TGG 0.626980 3_2:-79062655 Msa0428220:CDS
GCAGGAAATCATTGAAAAGG+GGG 0.627203 3_2:-79061400 Msa0428220:intron
GAAGGCGAGTCTCAAACTGA+AGG 0.630110 3_2:+79062677 None:intergenic
GCCAAAGCGAGACAATTGAA+AGG 0.630177 3_2:-79061064 Msa0428220:exon
TGTCAGATGGGACAGAAGGG+GGG 0.631248 3_2:+79062452 None:intergenic
TTCATCGGAAGCGTGAACGA+AGG 0.632521 3_2:+79063920 None:intergenic
GATCAGGGATTGTCTTGGAA+GGG 0.635602 3_2:+79062308 None:intergenic
TGATAACAAACCAGAAGACT+GGG 0.636071 3_2:-79062281 Msa0428220:CDS
TTGGATTGTATCTGAAAAGG+AGG 0.638329 3_2:-79063767 Msa0428220:CDS
CTGGTAGCCAGCAATACCCT+CGG 0.639757 3_2:+79061697 None:intergenic
ATGGTTGGATGACGAGCCAG+AGG 0.645028 3_2:-79062155 Msa0428220:CDS
GGCTAACTTCCTGTCCGAGG+AGG 0.645087 3_2:-79062350 Msa0428220:CDS
ATAAGAAAAGCAAGAAACAG+TGG 0.651157 3_2:+79060783 None:intergenic
GAGAATTCTAGTTGACGGCG+AGG 0.652459 3_2:-79062383 Msa0428220:CDS
CTTCTACGAATCGTTCGATG+AGG 0.652723 3_2:-79063803 Msa0428220:CDS
AAAGGCTAACTTCCTGTCCG+AGG 0.652948 3_2:-79062353 Msa0428220:CDS
AGCTGGGTTATCAATGTAGG+GGG 0.658344 3_2:+79061967 None:intergenic
CCATTAAGTATCATCATGAG+TGG 0.658399 3_2:-79061020 Msa0428220:three_prime_UTR
CTACAATTAGGTCAAACACT+CGG 0.659090 3_2:+79060885 None:intergenic
GGAAGCATTCTAAGAGTGAG+GGG 0.661084 3_2:-79062798 Msa0428220:CDS
AAACACAGAGACATTAGCAA+TGG 0.662420 3_2:-79064003 Msa0428220:exon
AAGCTGGGTTATCAATGTAG+GGG 0.662575 3_2:+79061966 None:intergenic
TGAGGATTGGGATGATGAGG+AGG 0.663099 3_2:-79062104 Msa0428220:CDS
CATTGAGAAAGAGAAACAGA+AGG 0.664396 3_2:-79061744 Msa0428220:CDS
GAAATTTGGACAATGCAGGA+CGG 0.665071 3_2:-79061851 Msa0428220:CDS
ACAGAAACTTCCCAAGCTGG+TGG 0.666572 3_2:-79061141 Msa0428220:CDS
GAGAGTGGTTGGAAACCCAA+GGG 0.671705 3_2:-79062604 Msa0428220:CDS
TCTGAAAAGGAGGAATACAA+TGG 0.671724 3_2:-79063757 Msa0428220:intron
CCCAGAAGCTGTAAAGCCAG+AGG 0.676697 3_2:-79062236 Msa0428220:CDS
TTCCGATGAAGCTGACGATG+CGG 0.679909 3_2:-79063907 Msa0428220:intron
CCCAGAGGCAACAAAACCTG+AGG 0.683397 3_2:-79062122 Msa0428220:CDS
GGTTACCCCTATCGCTATCG+CGG 0.684117 3_2:-79063970 Msa0428220:CDS
GTTTGGTCCAGACAGGTGCG+GGG 0.694516 3_2:-79062551 Msa0428220:CDS
GAAGAAGCTGAGAAACCTGA+AGG 0.695392 3_2:-79062178 Msa0428220:CDS
AGAGGAAATAGATGACCCAG+AGG 0.718864 3_2:-79062137 Msa0428220:CDS
AAACAGCCAATGCTCCACGT+AGG 0.734567 3_2:-79061089 Msa0428220:CDS
TGTCACGAAAACAACCACAA+CGG 0.747223 3_2:+79061341 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATAATGAATAATG+AGG + chr3_2:79061025-79061044 None:intergenic 10.0%
!! ATGAAATTAAATAGAAATTA+AGG - chr3_2:79061806-79061825 Msa0428220:CDS 10.0%
!!! TTATTTTTTTTTGTTTGTGA+TGG - chr3_2:79061034-79061053 Msa0428220:three_prime_UTR 15.0%
!!! CTTATTAATTAATTGTGTTT+TGG - chr3_2:79061218-79061237 Msa0428220:intron 15.0%
!!! GATAACTTTTTAAAAGAAAT+CGG - chr3_2:79061264-79061283 Msa0428220:intron 15.0%
!! ATTTCAAAAAATATTCCTAT+TGG + chr3_2:79061672-79061691 None:intergenic 15.0%
!!! AAATATAAGAATGATTTTTG+TGG - chr3_2:79061688-79061707 Msa0428220:intron 15.0%
!!! TACATTTTGATTTTTGTTTT+GGG - chr3_2:79062060-79062079 Msa0428220:CDS 15.0%
!!! AGATTTTTATATCAAAGCTA+GGG - chr3_2:79060849-79060868 Msa0428220:three_prime_UTR 20.0%
!!! AATTTTAAAAGGAAATCAAG+TGG + chr3_2:79061628-79061647 None:intergenic 20.0%
!!! TGATTTCCTTTTAAAATTAC+AGG - chr3_2:79061630-79061649 Msa0428220:intron 20.0%
!!! GATTTCCTTTTAAAATTACA+GGG - chr3_2:79061631-79061650 Msa0428220:intron 20.0%
!! TAACAGAAATAAAATGAAAG+TGG + chr3_2:79061852-79061871 None:intergenic 20.0%
!!! TTTATTTCTGTTATTCATTC+TGG - chr3_2:79061859-79061878 Msa0428220:CDS 20.0%
!!! TTATTTCTGTTATTCATTCT+GGG - chr3_2:79061860-79061879 Msa0428220:CDS 20.0%
!! AAATGTACATCAAAATTTGT+TGG + chr3_2:79062047-79062066 None:intergenic 20.0%
!!! GTACATTTTGATTTTTGTTT+TGG - chr3_2:79062059-79062078 Msa0428220:CDS 20.0%
!! TTACAATAATCTTATCTTTC+TGG + chr3_2:79063372-79063391 None:intergenic 20.0%
!! AGTATATAATCTTTCGAAAA+AGG + chr3_2:79063958-79063977 None:intergenic 20.0%
!! TTCGAAAGATTATATACTTA+AGG - chr3_2:79063960-79063979 Msa0428220:CDS 20.0%
!! AATTCATTACTAGAAAAAAG+AGG + chr3_2:79064053-79064072 None:intergenic 20.0%
!! CAGATTTTTATATCAAAGCT+AGG - chr3_2:79060848-79060867 Msa0428220:three_prime_UTR 25.0%
! AATGGTAAGTAAATTCGAAT+CGG - chr3_2:79061154-79061173 Msa0428220:CDS 25.0%
! GATTTAAGTAAAACCTAATC+TGG - chr3_2:79061359-79061378 Msa0428220:CDS 25.0%
! ATACATGTGTATAATATTGC+AGG - chr3_2:79061589-79061608 Msa0428220:intron 25.0%
!!! TCCTATTGGTTTTTATAGTT+GGG + chr3_2:79061658-79061677 None:intergenic 25.0%
!!! TTCCTATTGGTTTTTATAGT+TGG + chr3_2:79061659-79061678 None:intergenic 25.0%
! ACGGAATAATAATGAAAGTA+AGG - chr3_2:79061745-79061764 Msa0428220:CDS 25.0%
! AGAAATAAAATGAAAGTGGT+TGG + chr3_2:79061848-79061867 None:intergenic 25.0%
!! TATTTCTGTTATTCATTCTG+GGG - chr3_2:79061861-79061880 Msa0428220:CDS 25.0%
!! GCAAGTTCAATAAATTTTGA+AGG - chr3_2:79061945-79061964 Msa0428220:CDS 25.0%
! TCTTCATCAATAATTTCCAT+AGG + chr3_2:79062700-79062719 None:intergenic 25.0%
! TGATAATATTCTTATAGCGA+AGG - chr3_2:79063074-79063093 Msa0428220:intron 25.0%
! TCTTTCTCAATGTTAAACTT+GGG + chr3_2:79063141-79063160 None:intergenic 25.0%
!!! TTTTTTTACCTTACAGAAGA+AGG - chr3_2:79063300-79063319 Msa0428220:intron 25.0%
!! TTTAAATTTTCCTCCTCTTT+TGG - chr3_2:79063415-79063434 Msa0428220:intron 25.0%
!! TTTTCGTGACAATTTTCTTT+AGG - chr3_2:79063564-79063583 Msa0428220:intron 25.0%
!! ATTTTCTTTAGGCTCATATT+CGG - chr3_2:79063575-79063594 Msa0428220:intron 25.0%
! GATAAAATTTCACAACTTAC+AGG + chr3_2:79063611-79063630 None:intergenic 25.0%
! CTCGTTCATATTAATTTCAT+GGG - chr3_2:79063634-79063653 Msa0428220:intron 25.0%
! TCGTTCATATTAATTTCATG+GGG - chr3_2:79063635-79063654 Msa0428220:intron 25.0%
!! GTATTTTTCATTCACATTCA+TGG - chr3_2:79063658-79063677 Msa0428220:intron 25.0%
! AATCTTTCGAAAAAGGTAAA+AGG + chr3_2:79063951-79063970 None:intergenic 25.0%
! ATAATTGTACAGCTACAATT+AGG + chr3_2:79064023-79064042 None:intergenic 25.0%
! GAAAGGAAAGAGATATATTT+GGG + chr3_2:79064146-79064165 None:intergenic 25.0%
! AGAAAGGAAAGAGATATATT+TGG + chr3_2:79064147-79064166 None:intergenic 25.0%
! AAATATATCTCTTTCCTTTC+TGG - chr3_2:79064146-79064165 Msa0428220:five_prime_UTR 25.0%
! AAAAAAAACATAGGCTAGAT+CGG + chr3_2:79064172-79064191 None:intergenic 25.0%
TCAAAGAGAGAGTGATAAAT+GGG + chr3_2:79060790-79060809 None:intergenic 30.0%
TTCAAAGAGAGAGTGATAAA+TGG + chr3_2:79060791-79060810 None:intergenic 30.0%
GTGAATCAATAATCCAGATT+AGG + chr3_2:79061375-79061394 None:intergenic 30.0%
AAACAAACTAACTGCTATTC+TGG - chr3_2:79061457-79061476 Msa0428220:intron 30.0%
ATAATATTGCAGGCTAGATT+TGG - chr3_2:79061599-79061618 Msa0428220:intron 30.0%
ACCCAACTATAAAAACCAAT+AGG - chr3_2:79061654-79061673 Msa0428220:intron 30.0%
! ATTTTTGTGGCAATGTTGAA+GGG - chr3_2:79061701-79061720 Msa0428220:CDS 30.0%
! AAAGTAAGGATGAAAGCATA+GGG - chr3_2:79061759-79061778 Msa0428220:CDS 30.0%
!!! TGATTTTTGTTTTGGGTATC+AGG - chr3_2:79062067-79062086 Msa0428220:CDS 30.0%
TTCGTCAAGTTCTTTAACTA+TGG + chr3_2:79062167-79062186 None:intergenic 30.0%
TATTAAATATCTTCAACCCC+AGG - chr3_2:79062267-79062286 Msa0428220:CDS 30.0%
!! CACTTTGGGTTTTCAATTTT+GGG + chr3_2:79062832-79062851 None:intergenic 30.0%
!! ACACTTTGGGTTTTCAATTT+TGG + chr3_2:79062833-79062852 None:intergenic 30.0%
! ATTGATAACCCAGCTTATAA+GGG - chr3_2:79062934-79062953 Msa0428220:intron 30.0%
TGTCCAAATTTCAATACCAA+TGG + chr3_2:79063034-79063053 None:intergenic 30.0%
TATTGAAATTTGGACAATGC+AGG - chr3_2:79063038-79063057 Msa0428220:intron 30.0%
CTCTTTCTCAATGTTAAACT+TGG + chr3_2:79063142-79063161 None:intergenic 30.0%
AAGAGAGAAGATAGAGTAAA+TGG + chr3_2:79063237-79063256 None:intergenic 30.0%
ATGCATAAGAAAGAAAAGGA+AGG + chr3_2:79063271-79063290 None:intergenic 30.0%
CATCATGCATAAGAAAGAAA+AGG + chr3_2:79063275-79063294 None:intergenic 30.0%
!! ATCATAATGGATACCAAAAG+AGG + chr3_2:79063431-79063450 None:intergenic 30.0%
ATCCATTATGATTCACTTAC+TGG - chr3_2:79063439-79063458 Msa0428220:intron 30.0%
ATCCAGTAAGTGAATCATAA+TGG + chr3_2:79063444-79063463 None:intergenic 30.0%
ATTATGATTCACTTACTGGA+TGG - chr3_2:79063443-79063462 Msa0428220:intron 30.0%
AGAATTCCAATAGTCAGATT+TGG + chr3_2:79063527-79063546 None:intergenic 30.0%
GCTCGTTCATATTAATTTCA+TGG - chr3_2:79063633-79063652 Msa0428220:intron 30.0%
CGTTCATATTAATTTCATGG+GGG - chr3_2:79063636-79063655 Msa0428220:intron 30.0%
GAATGAAGACAACAAAGAAA+AGG - chr3_2:79063778-79063797 Msa0428220:CDS 30.0%
GAAAGGATTGAAACTTATAG+TGG - chr3_2:79063846-79063865 Msa0428220:intron 30.0%
CACTCATGATGATACTTAAT+GGG + chr3_2:79063875-79063894 None:intergenic 30.0%
! TCATCATGAGTGGTTAATTT+TGG - chr3_2:79063883-79063902 Msa0428220:intron 30.0%
! CATCATGAGTGGTTAATTTT+GGG - chr3_2:79063884-79063903 Msa0428220:intron 30.0%
! ATCATGAGTGGTTAATTTTG+GGG - chr3_2:79063885-79063904 Msa0428220:intron 30.0%
!! AGGAGTATATTTTCTCCTTT+TGG - chr3_2:79063980-79063999 Msa0428220:CDS 30.0%
! GTTTTTCACATAGCCAAATA+CGG - chr3_2:79064075-79064094 Msa0428220:five_prime_UTR 30.0%
! TTTTTCACATAGCCAAATAC+GGG - chr3_2:79064076-79064095 Msa0428220:five_prime_UTR 30.0%
ATAAGAAAAGCAAGAAACAG+TGG + chr3_2:79064113-79064132 None:intergenic 30.0%
GAGATATATTTGGGAACATT+AGG + chr3_2:79064137-79064156 None:intergenic 30.0%
!!! TCTAGCCTATGTTTTTTTTC+TGG - chr3_2:79064173-79064192 Msa0428220:five_prime_UTR 30.0%
ATCCACCAGAAAAAAAACAT+AGG + chr3_2:79064181-79064200 None:intergenic 30.0%
!!! TTTTCTGGTGGATTTAAGTT+TGG - chr3_2:79064188-79064207 Msa0428220:five_prime_UTR 30.0%
! AACAATTGGTTTAAGTCACG+TGG - chr3_2:79060712-79060731 Msa0428220:three_prime_UTR 35.0%
! GCAATATGATTGGTTGAAAC+AGG - chr3_2:79060738-79060757 Msa0428220:three_prime_UTR 35.0%
AAACACAGAGACATTAGCAA+TGG - chr3_2:79060890-79060909 Msa0428220:three_prime_UTR 35.0%
AAGAGGAAGCAATGAAGAAA+AGG + chr3_2:79060953-79060972 None:intergenic 35.0%
! CCTTTTCAGATACAATCCAA+CGG + chr3_2:79061126-79061145 None:intergenic 35.0%
TTGGATTGTATCTGAAAAGG+AGG - chr3_2:79061126-79061145 Msa0428220:CDS 35.0%
TCTGAAAAGGAGGAATACAA+TGG - chr3_2:79061136-79061155 Msa0428220:CDS 35.0%
! TGGGTCCCTGTAATTTTAAA+AGG + chr3_2:79061639-79061658 None:intergenic 35.0%
! GATTTTTGTGGCAATGTTGA+AGG - chr3_2:79061700-79061719 Msa0428220:CDS 35.0%
! GAAAGTAAGGATGAAAGCAT+AGG - chr3_2:79061758-79061777 Msa0428220:CDS 35.0%
AATTAATGCGAAACAGATCC+GGG + chr3_2:79062002-79062021 None:intergenic 35.0%
CAATTAATGCGAAACAGATC+CGG + chr3_2:79062003-79062022 None:intergenic 35.0%
TCTCCATATTCTGTCATGTT+TGG - chr3_2:79062325-79062344 Msa0428220:CDS 35.0%
AGGCATAAAAATCCAAAGAC+TGG - chr3_2:79062391-79062410 Msa0428220:CDS 35.0%
TCAACATACTTTCCAGTCTT+TGG + chr3_2:79062406-79062425 None:intergenic 35.0%
! CATGAGATAGTTTGTCAGAT+GGG + chr3_2:79062456-79062475 None:intergenic 35.0%
! ACATGAGATAGTTTGTCAGA+TGG + chr3_2:79062457-79062476 None:intergenic 35.0%
!! CTCATTATCAGGCTTTAGAA+TGG + chr3_2:79062488-79062507 None:intergenic 35.0%
!! CATTCTAAAGCCTGATAATG+AGG - chr3_2:79062486-79062505 Msa0428220:CDS 35.0%
AGAATTCTCACCTCATTATC+AGG + chr3_2:79062499-79062518 None:intergenic 35.0%
!! GTTTGTTATCATCAGGATCA+GGG + chr3_2:79062603-79062622 None:intergenic 35.0%
!! TCTTCTGGTTTGTTATCATC+AGG + chr3_2:79062610-79062629 None:intergenic 35.0%
ATGATAACAAACCAGAAGAC+TGG - chr3_2:79062611-79062630 Msa0428220:CDS 35.0%
TGATAACAAACCAGAAGACT+GGG - chr3_2:79062612-79062631 Msa0428220:CDS 35.0%
AAAAGGAACCCAGCTTATAA+GGG - chr3_2:79062892-79062911 Msa0428220:intron 35.0%
TAAGCTGGGTTATCAATGTA+GGG + chr3_2:79062931-79062950 None:intergenic 35.0%
ATAAGCTGGGTTATCAATGT+AGG + chr3_2:79062932-79062951 None:intergenic 35.0%
! CATTGATAACCCAGCTTATA+AGG - chr3_2:79062933-79062952 Msa0428220:intron 35.0%
TCCATATACCCTTATAAGCT+GGG + chr3_2:79062945-79062964 None:intergenic 35.0%
TTCCATATACCCTTATAAGC+TGG + chr3_2:79062946-79062965 None:intergenic 35.0%
! TCTCAAGTTCAAAGTACTCT+GGG + chr3_2:79062987-79063006 None:intergenic 35.0%
! TTCTCAAGTTCAAAGTACTC+TGG + chr3_2:79062988-79063007 None:intergenic 35.0%
! CTGCCATTGGTATTGAAATT+TGG - chr3_2:79063028-79063047 Msa0428220:intron 35.0%
AATCCTATCGTGAAACTACA+TGG - chr3_2:79063112-79063131 Msa0428220:intron 35.0%
TTTCCATGTAGTTTCACGAT+AGG + chr3_2:79063118-79063137 None:intergenic 35.0%
CATTGAGAAAGAGAAACAGA+AGG - chr3_2:79063149-79063168 Msa0428220:intron 35.0%
GAGAAGATAGAGTAAATGGA+AGG + chr3_2:79063233-79063252 None:intergenic 35.0%
!! ATAATGGATACCAAAAGAGG+AGG + chr3_2:79063428-79063447 None:intergenic 35.0%
TATACTTTATGCTTCTCTGC+AGG - chr3_2:79063475-79063494 Msa0428220:intron 35.0%
TCTGCAGGAAATCATTGAAA+AGG - chr3_2:79063490-79063509 Msa0428220:intron 35.0%
CTGCAGGAAATCATTGAAAA+GGG - chr3_2:79063491-79063510 Msa0428220:intron 35.0%
TGCAGGAAATCATTGAAAAG+GGG - chr3_2:79063492-79063511 Msa0428220:intron 35.0%
AAGCAACCAAATCTGACTAT+TGG - chr3_2:79063518-79063537 Msa0428220:intron 35.0%
CCACTCATGATGATACTTAA+TGG + chr3_2:79063876-79063895 None:intergenic 35.0%
CCATTAAGTATCATCATGAG+TGG - chr3_2:79063873-79063892 Msa0428220:intron 35.0%
! TCATGAGTGGTTAATTTTGG+GGG - chr3_2:79063886-79063905 Msa0428220:intron 35.0%
CTACAATTAGGTCAAACACT+CGG + chr3_2:79064011-79064030 None:intergenic 35.0%
!!! AGCCTATGTTTTTTTTCTGG+TGG - chr3_2:79064176-79064195 Msa0428220:five_prime_UTR 35.0%
! GGTGGATTTAAGTTTGGTAA+TGG - chr3_2:79064194-79064213 Msa0428220:five_prime_UTR 35.0%
GTGATGCAAAAAATCCAACG+TGG - chr3_2:79060684-79060703 Msa0428220:three_prime_UTR 40.0%
! ATTAGCAATGGTGGAACGAA+AGG - chr3_2:79060902-79060921 Msa0428220:three_prime_UTR 40.0%
!! ATGAGGATTTTGATAGCCGT+TGG - chr3_2:79061107-79061126 Msa0428220:CDS 40.0%
CCGTTGGATTGTATCTGAAA+AGG - chr3_2:79061123-79061142 Msa0428220:CDS 40.0%
! AATCGGTTTCTGCTACTACA+TGG - chr3_2:79061281-79061300 Msa0428220:intron 40.0%
ATGTTGAAGGGTTGTTGTGT+TGG - chr3_2:79061713-79061732 Msa0428220:CDS 40.0%
! GTTGTGTTGGCAATATGCTA+CGG - chr3_2:79061726-79061745 Msa0428220:CDS 40.0%
TTGCATAGTGTGATATGCGT+TGG + chr3_2:79061978-79061997 None:intergenic 40.0%
CATATCACACTATGCAAACC+CGG - chr3_2:79061981-79062000 Msa0428220:CDS 40.0%
!! TGGAAGCATTCTAAGAGTGA+GGG - chr3_2:79062094-79062113 Msa0428220:CDS 40.0%
TTCTTGTTAGTGAACCAGCA+AGG - chr3_2:79062134-79062153 Msa0428220:CDS 40.0%
TGACGAACCTGTCATTCTTA+AGG - chr3_2:79062180-79062199 Msa0428220:CDS 40.0%
GAACCTGTCATTCTTAAGGA+TGG - chr3_2:79062184-79062203 Msa0428220:CDS 40.0%
GATCCATCCTTAAGAATGAC+AGG + chr3_2:79062190-79062209 None:intergenic 40.0%
TATATGCACCACCACATTCA+AGG + chr3_2:79062249-79062268 None:intergenic 40.0%
GATTCGTTGTCAAATCCCTT+GGG + chr3_2:79062307-79062326 None:intergenic 40.0%
AGATTCGTTGTCAAATCCCT+TGG + chr3_2:79062308-79062327 None:intergenic 40.0%
GGACCAAACATGACAGAATA+TGG + chr3_2:79062331-79062350 None:intergenic 40.0%
! GAGGTGAGAATTCTAGTTGA+CGG - chr3_2:79062505-79062524 Msa0428220:CDS 40.0%
!! GGTTTGTTATCATCAGGATC+AGG + chr3_2:79062604-79062623 None:intergenic 40.0%
GAAAAGGAACCCAGCTTATA+AGG - chr3_2:79062891-79062910 Msa0428220:intron 40.0%
TCCACTTTCCCTTATAAGCT+GGG + chr3_2:79062903-79062922 None:intergenic 40.0%
AAGCTGGGTTATCAATGTAG+GGG + chr3_2:79062930-79062949 None:intergenic 40.0%
! ACCCAGCTTATAAGGGTATA+TGG - chr3_2:79062941-79062960 Msa0428220:intron 40.0%
! ATAAGGGTATATGGAAGCCT+CGG - chr3_2:79062950-79062969 Msa0428220:intron 40.0%
! AGTTCAAAGTACTCTGGGTT+TGG + chr3_2:79062982-79063001 None:intergenic 40.0%
TCAATACCAATGGCAGCAAT+AGG + chr3_2:79063024-79063043 None:intergenic 40.0%
GAAATTTGGACAATGCAGGA+CGG - chr3_2:79063042-79063061 Msa0428220:intron 40.0%
! ATGGAAGGATTTTCGTTACC+TGG + chr3_2:79063218-79063237 None:intergenic 40.0%
GTCAAATGCCTTCTTCTGTA+AGG + chr3_2:79063311-79063330 None:intergenic 40.0%
! TCTTATCTTTCTGGCCACTT+AGG + chr3_2:79063363-79063382 None:intergenic 40.0%
GCAGGAAATCATTGAAAAGG+GGG - chr3_2:79063493-79063512 Msa0428220:intron 40.0%
TGTCACGAAAACAACCACAA+CGG + chr3_2:79063555-79063574 None:intergenic 40.0%
TTCTTTAGGCTCATATTCGG+CGG - chr3_2:79063578-79063597 Msa0428220:intron 40.0%
! AACCAAGTCTTTTCTTCTGC+AGG - chr3_2:79063685-79063704 Msa0428220:intron 40.0%
TCCTTTCAATTGTCTCGCTT+TGG + chr3_2:79063833-79063852 None:intergenic 40.0%
! CATGAGTGGTTAATTTTGGG+GGG - chr3_2:79063887-79063906 Msa0428220:intron 40.0%
!! TTAATTTTGGGGGGCTTAGT+TGG - chr3_2:79063896-79063915 Msa0428220:intron 40.0%
! AAACACTCGGAGTATCCAAA+AGG + chr3_2:79063998-79064017 None:intergenic 40.0%
!!! CCAATTGTTTTGAGCCACGT+TGG + chr3_2:79060701-79060720 None:intergenic 45.0%
CCAACGTGGCTCAAAACAAT+TGG - chr3_2:79060698-79060717 Msa0428220:three_prime_UTR 45.0%
CACAGAGACATTAGCAATGG+TGG - chr3_2:79060893-79060912 Msa0428220:three_prime_UTR 45.0%
CTTCTACGAATCGTTCGATG+AGG - chr3_2:79061090-79061109 Msa0428220:CDS 45.0%
!! TGTTTTGGGTATCAGGTGTC+TGG - chr3_2:79062074-79062093 Msa0428220:CDS 45.0%
!! CTGGAAGCATTCTAAGAGTG+AGG - chr3_2:79062093-79062112 Msa0428220:CDS 45.0%
!! GGAAGCATTCTAAGAGTGAG+GGG - chr3_2:79062095-79062114 Msa0428220:CDS 45.0%
! TTTGAGACTCGCCTTCAGAA+TGG - chr3_2:79062223-79062242 Msa0428220:CDS 45.0%
CACATTCAAGGCCATTCTGA+AGG + chr3_2:79062237-79062256 None:intergenic 45.0%
! CTTCAGAATGGCCTTGAATG+TGG - chr3_2:79062235-79062254 Msa0428220:CDS 45.0%
ACAAGGTGCACTTCATCTTC+AGG - chr3_2:79062371-79062390 Msa0428220:CDS 45.0%
TTTGTCAGATGGGACAGAAG+GGG + chr3_2:79062446-79062465 None:intergenic 45.0%
GTTTGTCAGATGGGACAGAA+GGG + chr3_2:79062447-79062466 None:intergenic 45.0%
AGTTTGTCAGATGGGACAGA+AGG + chr3_2:79062448-79062467 None:intergenic 45.0%
AGAAGGCTCAAAATCCTCCT+CGG + chr3_2:79062560-79062579 None:intergenic 45.0%
GTCTTGGAAGGGATAAGAGA+AGG + chr3_2:79062577-79062596 None:intergenic 45.0%
GATCAGGGATTGTCTTGGAA+GGG + chr3_2:79062588-79062607 None:intergenic 45.0%
ATCAGGATCAGGGATTGTCT+TGG + chr3_2:79062593-79062612 None:intergenic 45.0%
GAAGAAGCTGAGAAACCTGA+AGG - chr3_2:79062715-79062734 Msa0428220:CDS 45.0%
AAGCTGAGAAACCTGAAGGA+TGG - chr3_2:79062719-79062738 Msa0428220:CDS 45.0%
TGAGAAACCTGAAGGATGGT+TGG - chr3_2:79062723-79062742 Msa0428220:CDS 45.0%
TCTGGGTCATCTATTTCCTC+TGG + chr3_2:79062757-79062776 None:intergenic 45.0%
AGAGGAAATAGATGACCCAG+AGG - chr3_2:79062756-79062775 Msa0428220:CDS 45.0%
AGGCAACAAAACCTGAGGAT+TGG - chr3_2:79062776-79062795 Msa0428220:CDS 45.0%
GGCAACAAAACCTGAGGATT+GGG - chr3_2:79062777-79062796 Msa0428220:CDS 45.0%
ATGATGAGGAGGATGGTGAA+TGG - chr3_2:79062800-79062819 Msa0428220:CDS 45.0%
TGATGAGGAGGATGGTGAAT+GGG - chr3_2:79062801-79062820 Msa0428220:CDS 45.0%
! TAAGCTGGGTTCCTTTTCGT+TGG + chr3_2:79062889-79062908 None:intergenic 45.0%
ACCCAGCTTATAAGGGAAAG+TGG - chr3_2:79062899-79062918 Msa0428220:intron 45.0%
CTCCACTTTCCCTTATAAGC+TGG + chr3_2:79062904-79062923 None:intergenic 45.0%
AGCTGGGTTATCAATGTAGG+GGG + chr3_2:79062929-79062948 None:intergenic 45.0%
! TAAGGGTATATGGAAGCCTC+GGG - chr3_2:79062951-79062970 Msa0428220:intron 45.0%
TTTGAGCCTATTGCTGCCAT+TGG - chr3_2:79063015-79063034 Msa0428220:intron 45.0%
AGAGAAACAGAAGGCTGAAG+AGG - chr3_2:79063158-79063177 Msa0428220:intron 45.0%
TGTCTGCGATCTTGTACAAG+AGG + chr3_2:79063333-79063352 None:intergenic 45.0%
! TCTTTCTGGCCACTTAGGAA+GGG + chr3_2:79063358-79063377 None:intergenic 45.0%
! ATCTTTCTGGCCACTTAGGA+AGG + chr3_2:79063359-79063378 None:intergenic 45.0%
TGGAATTCTTGCATCCGTTG+TGG - chr3_2:79063538-79063557 Msa0428220:intron 45.0%
TCTTTAGGCTCATATTCGGC+GGG - chr3_2:79063579-79063598 Msa0428220:intron 45.0%
AGCCTGCAGAAGAAAAGACT+TGG + chr3_2:79063690-79063709 None:intergenic 45.0%
! GTCTTTTCTTCTGCAGGCTA+AGG - chr3_2:79063691-79063710 Msa0428220:intron 45.0%
! TTTTCTTCTGCAGGCTAAGG+TGG - chr3_2:79063694-79063713 Msa0428220:intron 45.0%
AGTTTCTGTGCGTTCTGTGT+TGG + chr3_2:79063741-79063760 None:intergenic 45.0%
TTCATTCTCTCCACCAGCTT+GGG + chr3_2:79063765-79063784 None:intergenic 45.0%
GCCAAAGCGAGACAATTGAA+AGG - chr3_2:79063829-79063848 Msa0428220:intron 45.0%
AAGAAACAGTGGCTAGAGAC+AGG + chr3_2:79064102-79064121 None:intergenic 45.0%
CACGTGGCACGCAATATGAT+TGG - chr3_2:79060728-79060747 Msa0428220:three_prime_UTR 50.0%
! TTCATCGGAAGCGTGAACGA+AGG + chr3_2:79060976-79060995 None:intergenic 50.0%
TTCCGATGAAGCTGACGATG+CGG - chr3_2:79060986-79061005 Msa0428220:three_prime_UTR 50.0%
TACCGCATCGTCAGCTTCAT+CGG + chr3_2:79060991-79061010 None:intergenic 50.0%
!! AGTGAGGGGCACGATGATTT+TGG - chr3_2:79062109-79062128 Msa0428220:CDS 50.0%
ACTATGGCGTACTTCCTTGC+TGG + chr3_2:79062151-79062170 None:intergenic 50.0%
GAAGGCGAGTCTCAAACTGA+AGG + chr3_2:79062219-79062238 None:intergenic 50.0%
! CAGAATGGCCTTGAATGTGG+TGG - chr3_2:79062238-79062257 Msa0428220:CDS 50.0%
TATCTTCAACCCCAGGAGAG+TGG - chr3_2:79062274-79062293 Msa0428220:CDS 50.0%
GAGAGTGGTTGGAAACCCAA+GGG - chr3_2:79062289-79062308 Msa0428220:CDS 50.0%
CTGTCATGTTTGGTCCAGAC+AGG - chr3_2:79062335-79062354 Msa0428220:CDS 50.0%
ATGTTTGGTCCAGACAGGTG+CGG - chr3_2:79062340-79062359 Msa0428220:CDS 50.0%
TTGTCAGATGGGACAGAAGG+GGG + chr3_2:79062445-79062464 None:intergenic 50.0%
! GAGAATTCTAGTTGACGGCG+AGG - chr3_2:79062510-79062529 Msa0428220:CDS 50.0%
TGACGGCGAGGAGAAGAAAA+AGG - chr3_2:79062522-79062541 Msa0428220:CDS 50.0%
AAAGGCTAACTTCCTGTCCG+AGG - chr3_2:79062540-79062559 Msa0428220:CDS 50.0%
GGATCAGGGATTGTCTTGGA+AGG + chr3_2:79062589-79062608 None:intergenic 50.0%
AGCTTCTGGGTCAGGAATCT+TGG + chr3_2:79062647-79062666 None:intergenic 50.0%
!! CCTCTGGCTTTACAGCTTCT+GGG + chr3_2:79062660-79062679 None:intergenic 50.0%
!! TCCTCTGGCTTTACAGCTTC+TGG + chr3_2:79062661-79062680 None:intergenic 50.0%
AAGCTGTAAAGCCAGAGGAC+TGG - chr3_2:79062662-79062681 Msa0428220:CDS 50.0%
AGCTGTAAAGCCAGAGGACT+GGG - chr3_2:79062663-79062682 Msa0428220:CDS 50.0%
TCGTCATCCAACCATCCTTC+AGG + chr3_2:79062733-79062752 None:intergenic 50.0%
!!! CCTCAGGTTTTGTTGCCTCT+GGG + chr3_2:79062774-79062793 None:intergenic 50.0%
!!! TCCTCAGGTTTTGTTGCCTC+TGG + chr3_2:79062775-79062794 None:intergenic 50.0%
ACCTGAGGATTGGGATGATG+AGG - chr3_2:79062786-79062805 Msa0428220:CDS 50.0%
TCCTCATCATCCCAATCCTC+AGG + chr3_2:79062790-79062809 None:intergenic 50.0%
TGAGGATTGGGATGATGAGG+AGG - chr3_2:79062789-79062808 Msa0428220:CDS 50.0%
GATTGGGATGATGAGGAGGA+TGG - chr3_2:79062793-79062812 Msa0428220:CDS 50.0%
! CAGGGGCTGATTCACACTTT+GGG + chr3_2:79062846-79062865 None:intergenic 50.0%
AGTGGAAGAGGCCAACGAAA+AGG - chr3_2:79062875-79062894 Msa0428220:intron 50.0%
GCAGCAATAGGCTCAAAGTC+AGG + chr3_2:79063012-79063031 None:intergenic 50.0%
GAAACAGAAGGCTGAAGAGG+AGG - chr3_2:79063161-79063180 Msa0428220:intron 50.0%
GCAGACATTCCCTTCCTAAG+TGG - chr3_2:79063346-79063365 Msa0428220:intron 50.0%
ACAGAAACTTCCCAAGCTGG+TGG - chr3_2:79063752-79063771 Msa0428220:intron 50.0%
CTTCATTCTCTCCACCAGCT+TGG + chr3_2:79063766-79063785 None:intergenic 50.0%
AAACAGCCAATGCTCCACGT+AGG - chr3_2:79063804-79063823 Msa0428220:CDS 50.0%
! GGGGGGCTTAGTTGGATTTT+AGG - chr3_2:79063904-79063923 Msa0428220:intron 50.0%
ATAGGCTAGATCGGCCAGAA+AGG + chr3_2:79064163-79064182 None:intergenic 50.0%
GGTTACCCCTATCGCTATCG+CGG - chr3_2:79060923-79060942 Msa0428220:three_prime_UTR 55.0%
! GGAAGCGTGAACGAAGGAAG+AGG + chr3_2:79060970-79060989 None:intergenic 55.0%
TTCAACCCCAGGAGAGTGGT+TGG - chr3_2:79062278-79062297 Msa0428220:CDS 55.0%
GGTTTCCAACCACTCTCCTG+GGG + chr3_2:79062286-79062305 None:intergenic 55.0%
GGGTTTCCAACCACTCTCCT+GGG + chr3_2:79062287-79062306 None:intergenic 55.0%
TGGGTTTCCAACCACTCTCC+TGG + chr3_2:79062288-79062307 None:intergenic 55.0%
GGAGAGTGGTTGGAAACCCA+AGG - chr3_2:79062288-79062307 Msa0428220:CDS 55.0%
TGTTTGGTCCAGACAGGTGC+GGG - chr3_2:79062341-79062360 Msa0428220:CDS 55.0%
CAGAAGGGGGGAACTTGAGA+TGG + chr3_2:79062432-79062451 None:intergenic 55.0%
TGTCAGATGGGACAGAAGGG+GGG + chr3_2:79062444-79062463 None:intergenic 55.0%
GCTCAAAATCCTCCTCGGAC+AGG + chr3_2:79062555-79062574 None:intergenic 55.0%
GGCTTTACAGCTTCTGGGTC+AGG + chr3_2:79062655-79062674 None:intergenic 55.0%
CCCAGAAGCTGTAAAGCCAG+AGG - chr3_2:79062657-79062676 Msa0428220:CDS 55.0%
AAAGCCAGAGGACTGGGATG+AGG - chr3_2:79062669-79062688 Msa0428220:CDS 55.0%
CTGGGATGAGGATGCACCTA+TGG - chr3_2:79062681-79062700 Msa0428220:CDS 55.0%
ATGGTTGGATGACGAGCCAG+AGG - chr3_2:79062738-79062757 Msa0428220:CDS 55.0%
CCCAGAGGCAACAAAACCTG+AGG - chr3_2:79062771-79062790 Msa0428220:CDS 55.0%
! CCAGGGGCTGATTCACACTT+TGG + chr3_2:79062847-79062866 None:intergenic 55.0%
CCAAAGTGTGAATCAGCCCC+TGG - chr3_2:79062844-79062863 Msa0428220:intron 55.0%
TCTTCCACTCACCACAGCCA+GGG + chr3_2:79062864-79062883 None:intergenic 55.0%
! TCTGGGTTTGGAATCTCCCG+AGG + chr3_2:79062970-79062989 None:intergenic 55.0%
CTGGTAGCCAGCAATACCCT+CGG + chr3_2:79063199-79063218 None:intergenic 55.0%
TCTTCTGCAGGCTAAGGTGG+AGG - chr3_2:79063697-79063716 Msa0428220:intron 55.0%
CGCACAGAAACTTCCCAAGC+TGG - chr3_2:79063749-79063768 Msa0428220:intron 55.0%
GCTAGAGACAGGCCCGTATT+TGG + chr3_2:79064091-79064110 None:intergenic 55.0%
! GAGCACCGCGATAGCGATAG+GGG + chr3_2:79060931-79060950 None:intergenic 60.0%
!! GGAGCACCGCGATAGCGATA+GGG + chr3_2:79060932-79060951 None:intergenic 60.0%
!! AGGAGCACCGCGATAGCGAT+AGG + chr3_2:79060933-79060952 None:intergenic 60.0%
GTTTGGTCCAGACAGGTGCG+GGG - chr3_2:79062342-79062361 Msa0428220:CDS 60.0%
TTTGTTGCCCCGCACCTGTC+TGG + chr3_2:79062352-79062371 None:intergenic 60.0%
CAGGTGCGGGGCAACAAACA+AGG - chr3_2:79062354-79062373 Msa0428220:CDS 60.0%
GGCTAACTTCCTGTCCGAGG+AGG - chr3_2:79062543-79062562 Msa0428220:CDS 60.0%
GCTCTCTCGTCCCAGTCTTC+TGG + chr3_2:79062625-79062644 None:intergenic 60.0%
GCATCCTCATCCCAGTCCTC+TGG + chr3_2:79062676-79062695 None:intergenic 60.0%
TGTGAATCAGCCCCTGGCTG+TGG - chr3_2:79062850-79062869 Msa0428220:intron 60.0%
CTTCCACTCACCACAGCCAG+GGG + chr3_2:79062863-79062882 None:intergenic 60.0%
CTCTTCCACTCACCACAGCC+AGG + chr3_2:79062865-79062884 None:intergenic 60.0%
CTGGCTGTGGTGAGTGGAAG+AGG - chr3_2:79062863-79062882 Msa0428220:intron 60.0%
!! GCTGAGTCCGAGGGTATTGC+TGG - chr3_2:79063189-79063208 Msa0428220:intron 60.0%
!! CGAGGGTATTGCTGGCTACC+AGG - chr3_2:79063197-79063216 Msa0428220:intron 60.0%
CAGCCAATGCTCCACGTAGG+AGG - chr3_2:79063807-79063826 Msa0428220:CDS 60.0%
TGGCCTCCTACGTGGAGCAT+TGG + chr3_2:79063813-79063832 None:intergenic 60.0%
TCTCGCTTTGGCCTCCTACG+TGG + chr3_2:79063821-79063840 None:intergenic 60.0%
GAGGCAGCAGCTGAGTCCGA+GGG - chr3_2:79063180-79063199 Msa0428220:intron 65.0%
GCAGGCTAAGGTGGAGGCGA+AGG - chr3_2:79063703-79063722 Msa0428220:intron 65.0%
CAGCCCCTGGCTGTGGTGAG+TGG - chr3_2:79062857-79062876 Msa0428220:intron 70.0%
GGAGGCAGCAGCTGAGTCCG+AGG - chr3_2:79063179-79063198 Msa0428220:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 79060682 79064233 79060682 ID=Msa0428220;Name=Msa0428220
chr3_2 mRNA 79060682 79064233 79060682 ID=Msa0428220-mRNA-1;Parent=Msa0428220;Name=Msa0428220-mRNA-1;_AED=0.05;_eAED=0.05;_QI=227|1|1|1|1|1|6|386|541
chr3_2 exon 79060682 79061208 79060682 ID=Msa0428220-mRNA-1:exon:21205;Parent=Msa0428220-mRNA-1
chr3_2 exon 79061305 79061418 79061305 ID=Msa0428220-mRNA-1:exon:21204;Parent=Msa0428220-mRNA-1
chr3_2 exon 79061528 79061599 79061528 ID=Msa0428220-mRNA-1:exon:21203;Parent=Msa0428220-mRNA-1
chr3_2 exon 79061697 79062826 79061697 ID=Msa0428220-mRNA-1:exon:21202;Parent=Msa0428220-mRNA-1
chr3_2 exon 79063758 79063827 79063758 ID=Msa0428220-mRNA-1:exon:21201;Parent=Msa0428220-mRNA-1
chr3_2 exon 79063908 79064233 79063908 ID=Msa0428220-mRNA-1:exon:21200;Parent=Msa0428220-mRNA-1
chr3_2 five_prime_UTR 79064007 79064233 79064007 ID=Msa0428220-mRNA-1:five_prime_utr;Parent=Msa0428220-mRNA-1
chr3_2 CDS 79063908 79064006 79063908 ID=Msa0428220-mRNA-1:cds;Parent=Msa0428220-mRNA-1
chr3_2 CDS 79063758 79063827 79063758 ID=Msa0428220-mRNA-1:cds;Parent=Msa0428220-mRNA-1
chr3_2 CDS 79061697 79062826 79061697 ID=Msa0428220-mRNA-1:cds;Parent=Msa0428220-mRNA-1
chr3_2 CDS 79061528 79061599 79061528 ID=Msa0428220-mRNA-1:cds;Parent=Msa0428220-mRNA-1
chr3_2 CDS 79061305 79061418 79061305 ID=Msa0428220-mRNA-1:cds;Parent=Msa0428220-mRNA-1
chr3_2 CDS 79061068 79061208 79061068 ID=Msa0428220-mRNA-1:cds;Parent=Msa0428220-mRNA-1
chr3_2 three_prime_UTR 79060682 79061067 79060682 ID=Msa0428220-mRNA-1:three_prime_utr;Parent=Msa0428220-mRNA-1
Gene Sequence

>Msa0428220

ATGGTGGAACGAAAGGTTACCCCTATCGCTATCGCGGTGCTCCTTTTCTTCATTGCTTCCTCTTCCTTCGTTCACGCTTCCGATGAAGCTGACGATGCGATCTTCTACGAATCGTTCGATGAGGATTTTGATAGCCGTTGGATTGTATCTGAAAAGGAGGAATACAATGGTGTCTGGAAGCATTCTAAGAGTGAGGGGCACGATGATTTTGGACTTCTTGTTAGTGAACCAGCAAGGAAGTACGCCATAGTTAAAGAACTTGACGAACCTGTCATTCTTAAGGATGGATCAGTTGTCCTTCAGTTTGAGACTCGCCTTCAGAATGGCCTTGAATGTGGTGGTGCATATATTAAATATCTTCAACCCCAGGAGAGTGGTTGGAAACCCAAGGGATTTGACAACGAATCTCCATATTCTGTCATGTTTGGTCCAGACAGGTGCGGGGCAACAAACAAGGTGCACTTCATCTTCAGGCATAAAAATCCAAAGACTGGAAAGTATGTTGAACACCATCTCAAGTTCCCCCCTTCTGTCCCATCTGACAAACTATCTCATGTGTACACTGCCATTCTAAAGCCTGATAATGAGGTGAGAATTCTAGTTGACGGCGAGGAGAAGAAAAAGGCTAACTTCCTGTCCGAGGAGGATTTTGAGCCTTCTCTTATCCCTTCCAAGACAATCCCTGATCCTGATGATAACAAACCAGAAGACTGGGACGAGAGAGCCAAGATTCCTGACCCAGAAGCTGTAAAGCCAGAGGACTGGGATGAGGATGCACCTATGGAAATTATTGATGAAGAAGCTGAGAAACCTGAAGGATGGTTGGATGACGAGCCAGAGGAAATAGATGACCCAGAGGCAACAAAACCTGAGGATTGGGATGATGAGGAGGATGGTGAATGGGAAGCTCCCAAAATTGAAAACCCAAAGTGTGAATCAGCCCCTGGCTGTGGTGAGTGGAAGAGGCCAACGAAAAGGAACCCAGCTTATAAGGGAAAGTGGAGTGCCCCCTACATTGATAACCCAGCTTATAAGGGTATATGGAAGCCTCGGGAGATTCCAAACCCAGAGTACTTTGAACTTGAGAAACCTGACTTTGAGCCTATTGCTGCCATTGGTATTGAAATTTGGACAATGCAGGACGGCATTCTATTTGATAATATTCTTATAGCGAAGGATGATAAGATTGCAGAATCCTATCGTGAAACTACATGGAAACCCAAGTTTAACATTGAGAAAGAGAAACAGAAGGCTGAAGAGGAGGCAGCAGCTGAGTCCGAGGGTATTGCTGGCTACCAGAAGAAGGCATTTGACCTCTTGTACAAGATCGCAGACATTCCCTTCCTAAGTGGCCAGAAAGATAAGATTATTGAAATCATTGAAAAGGGGGAGAAGCAACCAAATCTGACTATTGGAATTCTTGCATCCGTTGTGGTTGTTTTCGTGACAATTTTCTTTAGGCTCATATTCGGCGGGAAGAAACCTGCTAAGGTGGAGGCGAAGGTAGAGAAGAAGACCAACACAGAACGCACAGAAACTTCCCAAGCTGGTGGAGAGAATGAAGACAACAAAGAAAAGGAAGAAACAGCCAATGCTCCACGTAGGAGGCCAAAGCGAGACAATTGA
Protein sequence

>Msa0428220

MVERKVTPIAIAVLLFFIASSSFVHASDEADDAIFYESFDEDFDSRWIVSEKEEYNGVWKHSKSEGHDDFGLLVSEPARKYAIVKELDEPVILKDGSVVLQFETRLQNGLECGGAYIKYLQPQESGWKPKGFDNESPYSVMFGPDRCGATNKVHFIFRHKNPKTGKYVEHHLKFPPSVPSDKLSHVYTAILKPDNEVRILVDGEEKKKANFLSEEDFEPSLIPSKTIPDPDDNKPEDWDERAKIPDPEAVKPEDWDEDAPMEIIDEEAEKPEGWLDDEPEEIDDPEATKPEDWDDEEDGEWEAPKIENPKCESAPGCGEWKRPTKRNPAYKGKWSAPYIDNPAYKGIWKPREIPNPEYFELEKPDFEPIAAIGIEIWTMQDGILFDNILIAKDDKIAESYRETTWKPKFNIEKEKQKAEEEAAAESEGIAGYQKKAFDLLYKIADIPFLSGQKDKIIEIIEKGEKQPNLTIGILASVVVVFVTIFFRLIFGGKKPAKVEAKVEKKTNTERTETSQAGGENEDNKEKEETANAPRRRPKRDN*