AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0440050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTACATTGCCCGTTTAA+AGG 0.179011 3_3:-6561513 None:intergenic
TTAAATTTGCAGCTGAATTT+TGG 0.183575 3_3:+6561469 Msa0440050:three_prime_UTR
ATGAGAAGTCTTTCTCAATT+TGG 0.210563 3_3:+6561382 Msa0440050:three_prime_UTR
TTGAGCTCTCTTTGGGATTC+CGG 0.235911 3_3:+6560956 Msa0440050:three_prime_UTR
TATTGGACTTGAGCTCTCTT+TGG 0.236692 3_3:+6560948 Msa0440050:three_prime_UTR
TTACCAGAACAGTCTTAATT+TGG 0.241556 3_3:-6561220 None:intergenic
GAGGTTGAAATTTGAATATT+CGG 0.267470 3_3:-6561180 None:intergenic
ATCTACAACCTCGAATATTC+AGG 0.269236 3_3:-6561347 None:intergenic
GGCAAGAGAAGAAGGCTTAT+TGG 0.295513 3_3:+6560931 Msa0440050:three_prime_UTR
AGCTTCATCAACCTTTGATA+TGG 0.306932 3_3:+6560997 Msa0440050:three_prime_UTR
TGAGCTCTCTTTGGGATTCC+GGG 0.318903 3_3:+6560957 Msa0440050:three_prime_UTR
AAACCAAATTAAGACTGTTC+TGG 0.322742 3_3:+6561217 Msa0440050:three_prime_UTR
CTTTAAACGGGCAATGTAAT+GGG 0.345412 3_3:+6561514 Msa0440050:three_prime_UTR
ATTGGACTTGAGCTCTCTTT+GGG 0.350049 3_3:+6560949 Msa0440050:three_prime_UTR
CTTTCTCAATTTGGTTACTT+TGG 0.380167 3_3:+6561391 Msa0440050:three_prime_UTR
AGCACATCTTCTTCCTCTTC+AGG 0.394518 3_3:+6558740 Msa0440050:CDS
TTTGTTGGAAAACCTTTAAA+CGG 0.402330 3_3:+6561501 Msa0440050:three_prime_UTR
CCTTTAAACGGGCAATGTAA+TGG 0.403434 3_3:+6561513 Msa0440050:three_prime_UTR
TTCCTCGATCGATGATCATT+AGG 0.414996 3_3:-6561555 None:intergenic
TTGTTGGAAAACCTTTAAAC+GGG 0.455903 3_3:+6561502 Msa0440050:three_prime_UTR
TGCAGTGCCCTGAATATTCG+AGG 0.457234 3_3:+6561339 Msa0440050:intron
ATCTCAAAACAGAGAGCTCC+AGG 0.461402 3_3:-6561612 None:intergenic
TTGATATAAAGCTGTCTCAA+TGG 0.466832 3_3:-6560177 None:intergenic
TCAGCTTCTCATGTCATGAT+AGG 0.467204 3_3:+6560849 Msa0440050:CDS
ATCAAGGACTGAAGGAAGCC+AGG 0.467987 3_3:+6560195 Msa0440050:CDS
GCTCTTTACAGATTGAATGA+TGG 0.469171 3_3:+6560798 Msa0440050:intron
TGAAGAGGAAGAAGATGTGC+TGG 0.471374 3_3:-6558738 None:intergenic
ATTGAGACAGCTTTATATCA+AGG 0.473665 3_3:+6560179 Msa0440050:CDS
TATCAGTTGGCAAGAGAAGA+AGG 0.478820 3_3:+6560923 Msa0440050:three_prime_UTR
CAAAATGTTAACTTTCAGCC+TGG 0.483662 3_3:+6561594 Msa0440050:three_prime_UTR
ACATGAGAAGCTGAATGGTT+AGG 0.490175 3_3:-6560840 None:intergenic
AAATTGAGAAAGACTTCTCA+TGG 0.496464 3_3:-6561380 None:intergenic
GATCATCGATCGAGGAAACT+AGG 0.505088 3_3:+6561561 Msa0440050:three_prime_UTR
AGTTGATCTTGCACATTGAA+AGG 0.507248 3_3:+6560219 Msa0440050:CDS
AATGTAATGGGGCGTTATGA+TGG 0.509551 3_3:+6561526 Msa0440050:three_prime_UTR
AAAGGTTGATGAAGCTCAGA+TGG 0.510421 3_3:-6560990 None:intergenic
AATGTGCAAGATCAACTCCC+TGG 0.525919 3_3:-6560213 None:intergenic
TCTCACTTTCACCATATCAA+AGG 0.544762 3_3:-6561008 None:intergenic
TCATGACATGAGAAGCTGAA+TGG 0.547992 3_3:-6560845 None:intergenic
TCTACAACCTCGAATATTCA+GGG 0.550853 3_3:-6561346 None:intergenic
TGCTCAGAGCTTTATACCAA+TGG 0.558253 3_3:+6560260 Msa0440050:CDS
TCAGATGGGATTTGAACTCC+CGG 0.564088 3_3:-6560975 None:intergenic
AATTGAGAAAGACTTCTCAT+GGG 0.567965 3_3:-6561379 None:intergenic
GATTGAATGATGGCTCCTGC+TGG 0.574590 3_3:+6560808 Msa0440050:intron
GTTAGGTAGCTCTAACCAGC+AGG 0.574645 3_3:-6560823 None:intergenic
TCAAGGACTGAAGGAAGCCA+GGG 0.576293 3_3:+6560196 Msa0440050:CDS
AGCTTTATATCAAGGACTGA+AGG 0.592568 3_3:+6560187 Msa0440050:CDS
TGTGAGCAAGTACTTACCAT+TGG 0.601534 3_3:-6560276 None:intergenic
GAAGAGGAAGAAGATGTGCT+GGG 0.614172 3_3:-6558737 None:intergenic
AAGGTTGATGAAGCTCAGAT+GGG 0.621898 3_3:-6560989 None:intergenic
TTGTAGTTTCAGCATCAGCA+TGG 0.627355 3_3:-6561416 None:intergenic
CTTGAGAAAAGAATGTGATG+AGG 0.631451 3_3:+6559993 Msa0440050:CDS
TTTAAACGGGCAATGTAATG+GGG 0.632286 3_3:+6561515 Msa0440050:three_prime_UTR
GTGTGCCTGTGCAGAAAACA+CGG 0.644924 3_3:+6559968 Msa0440050:intron
TGCCTAATGATCATCGATCG+AGG 0.655886 3_3:+6561553 Msa0440050:three_prime_UTR
ATTGAGAAAGACTTCTCATG+GGG 0.661803 3_3:-6561378 None:intergenic
TGTGCCTGTGCAGAAAACAC+GGG 0.744854 3_3:+6559969 Msa0440050:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATCACAGTTTTTAATA+TGG - chr3_3:6558855-6558874 None:intergenic 15.0%
!! AAATTAGAAAATCTACATTA+GGG - chr3_3:6558979-6558998 None:intergenic 15.0%
!!! AATTAGTATGAATTTTTTCA+AGG + chr3_3:6559065-6559084 Msa0440050:intron 15.0%
!!! TGAAATACATTTTAATTTGA+AGG + chr3_3:6560033-6560052 Msa0440050:intron 15.0%
!!! TTTTTATATGTTTTTTTGAC+AGG - chr3_3:6561693-6561712 None:intergenic 15.0%
!! TGTCAAAAAAACATATAAAA+AGG + chr3_3:6561692-6561711 Msa0440050:three_prime_UTR 15.0%
!! GAAATTAGAAAATCTACATT+AGG - chr3_3:6558980-6558999 None:intergenic 20.0%
!!! TACTAATTGTTAGTATTTTC+TGG - chr3_3:6559053-6559072 None:intergenic 20.0%
!!! GTTTTCTGTAACAAAAAATA+TGG - chr3_3:6559110-6559129 None:intergenic 20.0%
!!! TTTTCATGGAGTATATTAAA+CGG + chr3_3:6559535-6559554 Msa0440050:intron 20.0%
!!! GATGAACAAATCAATTTTTT+GGG + chr3_3:6560480-6560499 Msa0440050:intron 20.0%
!!! TACTTTTACATCTGATATAT+AGG - chr3_3:6560550-6560569 None:intergenic 20.0%
!! AAACCAAATTATGATAAGTA+GGG - chr3_3:6561127-6561146 None:intergenic 20.0%
!! TAGAATTCATTCTATATTGT+AGG + chr3_3:6561154-6561173 Msa0440050:intron 20.0%
!!! AAAAACATATAAAAAGGTGT+TGG + chr3_3:6561698-6561717 Msa0440050:three_prime_UTR 20.0%
! TAGAGAATCTATAAATTCCA+TGG - chr3_3:6559015-6559034 None:intergenic 25.0%
! ATATCCAAATACATCTGATT+AGG - chr3_3:6559265-6559284 None:intergenic 25.0%
! ACATTCAGAAGAATAACAAA+GGG - chr3_3:6559732-6559751 None:intergenic 25.0%
! GATGTGACTAATTGTAAAAA+GGG - chr3_3:6559757-6559776 None:intergenic 25.0%
! TTAATTGAGTAGATGAAAAC+AGG + chr3_3:6560316-6560335 Msa0440050:intron 25.0%
! TCCATTCATTATTTGATGTA+TGG + chr3_3:6560406-6560425 Msa0440050:intron 25.0%
! ACCATACATCAAATAATGAA+TGG - chr3_3:6560410-6560429 None:intergenic 25.0%
!!! GGATGAACAAATCAATTTTT+TGG + chr3_3:6560479-6560498 Msa0440050:intron 25.0%
! TTGTACTTTGGTCAATAAAA+AGG + chr3_3:6561096-6561115 Msa0440050:intron 25.0%
! TGTACTTTGGTCAATAAAAA+GGG + chr3_3:6561097-6561116 Msa0440050:intron 25.0%
! CAAACCAAATTATGATAAGT+AGG - chr3_3:6561128-6561147 None:intergenic 25.0%
! GAGGTTGAAATTTGAATATT+CGG - chr3_3:6561183-6561202 None:intergenic 25.0%
!!! ACAGTCTTAATTTGGTTTTA+AGG - chr3_3:6561215-6561234 None:intergenic 25.0%
!! GAATAAGAAGTATGTTAGAA+AGG - chr3_3:6561319-6561338 None:intergenic 25.0%
!! TTAAATTTGCAGCTGAATTT+TGG + chr3_3:6561469-6561488 Msa0440050:three_prime_UTR 25.0%
! TTTGTTGGAAAACCTTTAAA+CGG + chr3_3:6561501-6561520 Msa0440050:three_prime_UTR 25.0%
!!! ATATAAAAAGGTGTTGGAAA+TGG + chr3_3:6561704-6561723 Msa0440050:three_prime_UTR 25.0%
AAAAATACCTTTGACACAGA+GGG - chr3_3:6558810-6558829 None:intergenic 30.0%
TAAAAATACCTTTGACACAG+AGG - chr3_3:6558811-6558830 None:intergenic 30.0%
!!! TCTGTGTCAAAGGTATTTTT+AGG + chr3_3:6558810-6558829 Msa0440050:intron 30.0%
TGAGAAAATTGTCAATCAAG+TGG - chr3_3:6558936-6558955 None:intergenic 30.0%
! ATTTCATATTTTGCATGCCA+TGG + chr3_3:6558995-6559014 Msa0440050:intron 30.0%
! ATATGGCAGTTAGTATCTTT+TGG - chr3_3:6559093-6559112 None:intergenic 30.0%
GACAACTTTGTAAAACAGTT+TGG - chr3_3:6559506-6559525 None:intergenic 30.0%
! TTGTCTGCAACAAATTTTCA+TGG + chr3_3:6559521-6559540 Msa0440050:intron 30.0%
!!! CAACATTTTTAGTAAAGCCA+GGG - chr3_3:6559686-6559705 None:intergenic 30.0%
!!! ACAACATTTTTAGTAAAGCC+AGG - chr3_3:6559687-6559706 None:intergenic 30.0%
!!! GGGGAAATTTGCTTTTTATT+TGG - chr3_3:6559712-6559731 None:intergenic 30.0%
CATTCAGAAGAATAACAAAG+GGG - chr3_3:6559731-6559750 None:intergenic 30.0%
CACATTCAGAAGAATAACAA+AGG - chr3_3:6559733-6559752 None:intergenic 30.0%
GGATGTGACTAATTGTAAAA+AGG - chr3_3:6559758-6559777 None:intergenic 30.0%
! TAGTCAAAGTATCCATATGT+TGG - chr3_3:6559855-6559874 None:intergenic 30.0%
CATATGGATACTTTGACTAT+TGG + chr3_3:6559856-6559875 Msa0440050:intron 30.0%
ACATTGTAGGATCCTTTATT+GGG + chr3_3:6559915-6559934 Msa0440050:intron 30.0%
! TTTACTTTGAAAAATGCAGC+TGG + chr3_3:6560136-6560155 Msa0440050:intron 30.0%
TTGATATAAAGCTGTCTCAA+TGG - chr3_3:6560180-6560199 None:intergenic 30.0%
! ATTGAGACAGCTTTATATCA+AGG + chr3_3:6560179-6560198 Msa0440050:CDS 30.0%
TTCATCTACTCAATTAAGTG+TGG - chr3_3:6560313-6560332 None:intergenic 30.0%
AAAATGCATGTAACATACAG+AGG + chr3_3:6560347-6560366 Msa0440050:intron 30.0%
AAATGCATGTAACATACAGA+GGG + chr3_3:6560348-6560367 Msa0440050:intron 30.0%
!!! TGTTTTAGTGTCCAAATTTG+AGG + chr3_3:6560458-6560477 Msa0440050:intron 30.0%
! GATTTGTTCATCCTCAAATT+TGG - chr3_3:6560472-6560491 None:intergenic 30.0%
TTACATCTGATATATAGGCT+CGG - chr3_3:6560545-6560564 None:intergenic 30.0%
! TCATTTTCCATATGATTAGC+TGG + chr3_3:6560599-6560618 Msa0440050:intron 30.0%
!! CATTTTCCATATGATTAGCT+GGG + chr3_3:6560600-6560619 Msa0440050:intron 30.0%
!! TCTGTATTCACAACTAGTTT+TGG + chr3_3:6560685-6560704 Msa0440050:intron 30.0%
!!! TATTCACAACTAGTTTTGGT+TGG + chr3_3:6560689-6560708 Msa0440050:intron 30.0%
CATTGATCAGATATATGCAT+CGG + chr3_3:6560725-6560744 Msa0440050:intron 30.0%
!! TCTTCACAAAGATGTTTTTG+AGG + chr3_3:6560872-6560891 Msa0440050:CDS 30.0%
!! CTTTTTAAGAGTCTATCAGT+TGG + chr3_3:6560910-6560929 Msa0440050:three_prime_UTR 30.0%
GTAGGTCATTAGAAATAGAA+TGG - chr3_3:6561035-6561054 None:intergenic 30.0%
AAAATGCTCACTTTGTACTT+TGG + chr3_3:6561084-6561103 Msa0440050:intron 30.0%
AGTCCCTACTTATCATAATT+TGG + chr3_3:6561121-6561140 Msa0440050:intron 30.0%
AAACCAAATTAAGACTGTTC+TGG + chr3_3:6561217-6561236 Msa0440050:three_prime_UTR 30.0%
TTACCAGAACAGTCTTAATT+TGG - chr3_3:6561223-6561242 None:intergenic 30.0%
GTATGTTAGAAAGGATATGT+TGG - chr3_3:6561310-6561329 None:intergenic 30.0%
AATTGAGAAAGACTTCTCAT+GGG - chr3_3:6561382-6561401 None:intergenic 30.0%
AAATTGAGAAAGACTTCTCA+TGG - chr3_3:6561383-6561402 None:intergenic 30.0%
ATGAGAAGTCTTTCTCAATT+TGG + chr3_3:6561382-6561401 Msa0440050:three_prime_UTR 30.0%
CTTTCTCAATTTGGTTACTT+TGG + chr3_3:6561391-6561410 Msa0440050:three_prime_UTR 30.0%
! TAAAGGTTTTCCAACAAAAC+CGG - chr3_3:6561499-6561518 None:intergenic 30.0%
TTGTTGGAAAACCTTTAAAC+GGG + chr3_3:6561502-6561521 Msa0440050:three_prime_UTR 30.0%
! ACTTTTCAGTTTACCTGAAG+AGG - chr3_3:6558756-6558775 None:intergenic 35.0%
AAAATACCTTTGACACAGAG+GGG - chr3_3:6558809-6558828 None:intergenic 35.0%
! AACCACATTTTCTCATTCCA+TGG + chr3_3:6559195-6559214 Msa0440050:intron 35.0%
AGTTTCTTTACACTGTAACC+TGG - chr3_3:6559238-6559257 None:intergenic 35.0%
CTTGCCTAATCAGATGTATT+TGG + chr3_3:6559258-6559277 Msa0440050:intron 35.0%
GTTGTAGTAAATACCAGCTT+TGG - chr3_3:6559313-6559332 None:intergenic 35.0%
TTCACATGCAGTAAGTGAAA+AGG - chr3_3:6559429-6559448 None:intergenic 35.0%
GCATGTGAATTATATCCATG+TGG + chr3_3:6559440-6559459 Msa0440050:intron 35.0%
GAATACAAATCGTAACCACA+TGG - chr3_3:6559458-6559477 None:intergenic 35.0%
ATAAGTAAGAGATCAATCCC+TGG + chr3_3:6559666-6559685 Msa0440050:intron 35.0%
AATTAGTCACATCCCTTATG+TGG + chr3_3:6559764-6559783 Msa0440050:intron 35.0%
ATTCGTCAATCTCCACATAA+GGG - chr3_3:6559779-6559798 None:intergenic 35.0%
AATTCGTCAATCTCCACATA+AGG - chr3_3:6559780-6559799 None:intergenic 35.0%
CATATGTTGGATCCATAACT+TGG - chr3_3:6559842-6559861 None:intergenic 35.0%
CAAGTTATGGATCCAACATA+TGG + chr3_3:6559840-6559859 Msa0440050:intron 35.0%
GACAATCTGAATCACATTGT+AGG + chr3_3:6559902-6559921 Msa0440050:intron 35.0%
CACATTGTAGGATCCTTTAT+TGG + chr3_3:6559914-6559933 Msa0440050:intron 35.0%
AACGTTAAAGAGCCCAATAA+AGG - chr3_3:6559930-6559949 None:intergenic 35.0%
CTTGAGAAAAGAATGTGATG+AGG + chr3_3:6559993-6560012 Msa0440050:CDS 35.0%
TTGAAGGTTGCGACTTAAAA+AGG + chr3_3:6560049-6560068 Msa0440050:intron 35.0%
! AGCTTTATATCAAGGACTGA+AGG + chr3_3:6560187-6560206 Msa0440050:CDS 35.0%
AGTTGATCTTGCACATTGAA+AGG + chr3_3:6560219-6560238 Msa0440050:CDS 35.0%
AATGCATGTAACATACAGAG+GGG + chr3_3:6560349-6560368 Msa0440050:intron 35.0%
TAAGTGGGATAGAGAAGTTT+CGG + chr3_3:6560381-6560400 Msa0440050:intron 35.0%
GAACAGGAATCAAGTCTAAA+TGG - chr3_3:6560667-6560686 None:intergenic 35.0%
ACTAGTTGTGAATACAGAAC+AGG - chr3_3:6560683-6560702 None:intergenic 35.0%
CGATGCATATATCTGATCAA+TGG - chr3_3:6560727-6560746 None:intergenic 35.0%
GCTCTTTACAGATTGAATGA+TGG + chr3_3:6560798-6560817 Msa0440050:intron 35.0%
AGCTTCATCAACCTTTGATA+TGG + chr3_3:6560997-6561016 Msa0440050:three_prime_UTR 35.0%
TCTCACTTTCACCATATCAA+AGG - chr3_3:6561011-6561030 None:intergenic 35.0%
TGAAGTCTTAATTGCAGAGT+AGG - chr3_3:6561053-6561072 None:intergenic 35.0%
TCTACAACCTCGAATATTCA+GGG - chr3_3:6561349-6561368 None:intergenic 35.0%
ATCTACAACCTCGAATATTC+AGG - chr3_3:6561350-6561369 None:intergenic 35.0%
ATTGAGAAAGACTTCTCATG+GGG - chr3_3:6561381-6561400 None:intergenic 35.0%
! AAAGGTTTTCCAACAAAACC+GGG - chr3_3:6561498-6561517 None:intergenic 35.0%
CTTTAAACGGGCAATGTAAT+GGG + chr3_3:6561514-6561533 Msa0440050:three_prime_UTR 35.0%
TTTAAACGGGCAATGTAATG+GGG + chr3_3:6561515-6561534 Msa0440050:three_prime_UTR 35.0%
CAAAATGTTAACTTTCAGCC+TGG + chr3_3:6561594-6561613 Msa0440050:three_prime_UTR 35.0%
TTCCGAACCATTAGTCCTTA+TGG + chr3_3:6558898-6558917 Msa0440050:intron 40.0%
AGCCATGGAATGAGAAAATG+TGG - chr3_3:6559200-6559219 None:intergenic 40.0%
AGCAAATCTTGTTGAAGCCA+TGG - chr3_3:6559215-6559234 None:intergenic 40.0%
TTGATTTCTTTCGCCAAAGC+TGG + chr3_3:6559297-6559316 Msa0440050:intron 40.0%
ACTTTGTTCCTTTGCTCCTA+AGG + chr3_3:6559374-6559393 Msa0440050:intron 40.0%
CATAGAATCCAAACCACCTT+AGG - chr3_3:6559393-6559412 None:intergenic 40.0%
! CTTTGAAAAATGCAGCTGGA+TGG + chr3_3:6560140-6560159 Msa0440050:intron 40.0%
! TGCTCAGAGCTTTATACCAA+TGG + chr3_3:6560260-6560279 Msa0440050:CDS 40.0%
TGTGAGCAAGTACTTACCAT+TGG - chr3_3:6560279-6560298 None:intergenic 40.0%
TACTTGCTCACAGCATTCTT+CGG + chr3_3:6560287-6560306 Msa0440050:intron 40.0%
AGAGGGGAGTAAGAATTAAG+TGG + chr3_3:6560365-6560384 Msa0440050:intron 40.0%
GAGGGGAGTAAGAATTAAGT+GGG + chr3_3:6560366-6560385 Msa0440050:intron 40.0%
!! GGTATGTTGACTTCTCATGT+AGG - chr3_3:6560524-6560543 None:intergenic 40.0%
AGATGACCCAGCTAATCATA+TGG - chr3_3:6560609-6560628 None:intergenic 40.0%
! GCTGGGTCATCTTATTTCTT+TGG + chr3_3:6560617-6560636 Msa0440050:intron 40.0%
CTGTAAAGAGCAATGCACAT+GGG - chr3_3:6560789-6560808 None:intergenic 40.0%
TCTGTAAAGAGCAATGCACA+TGG - chr3_3:6560790-6560809 None:intergenic 40.0%
ACATGAGAAGCTGAATGGTT+AGG - chr3_3:6560843-6560862 None:intergenic 40.0%
TCATGACATGAGAAGCTGAA+TGG - chr3_3:6560848-6560867 None:intergenic 40.0%
TCAGCTTCTCATGTCATGAT+AGG + chr3_3:6560849-6560868 Msa0440050:CDS 40.0%
TATCAGTTGGCAAGAGAAGA+AGG + chr3_3:6560923-6560942 Msa0440050:three_prime_UTR 40.0%
TATTGGACTTGAGCTCTCTT+TGG + chr3_3:6560948-6560967 Msa0440050:three_prime_UTR 40.0%
ATTGGACTTGAGCTCTCTTT+GGG + chr3_3:6560949-6560968 Msa0440050:three_prime_UTR 40.0%
!! AAGGTTGATGAAGCTCAGAT+GGG - chr3_3:6560992-6561011 None:intergenic 40.0%
!! AAAGGTTGATGAAGCTCAGA+TGG - chr3_3:6560993-6561012 None:intergenic 40.0%
!!! GGTTTTAAGGTATTCAGCAG+AGG - chr3_3:6561202-6561221 None:intergenic 40.0%
TTGTAGTTTCAGCATCAGCA+TGG - chr3_3:6561419-6561438 None:intergenic 40.0%
CCATTACATTGCCCGTTTAA+AGG - chr3_3:6561516-6561535 None:intergenic 40.0%
CCTTTAAACGGGCAATGTAA+TGG + chr3_3:6561513-6561532 Msa0440050:three_prime_UTR 40.0%
! AATGTAATGGGGCGTTATGA+TGG + chr3_3:6561526-6561545 Msa0440050:three_prime_UTR 40.0%
TTCCTCGATCGATGATCATT+AGG - chr3_3:6561558-6561577 None:intergenic 40.0%
GAAGAGGAAGAAGATGTGCT+GGG - chr3_3:6558740-6558759 None:intergenic 45.0%
TGAAGAGGAAGAAGATGTGC+TGG - chr3_3:6558741-6558760 None:intergenic 45.0%
AGCACATCTTCTTCCTCTTC+AGG + chr3_3:6558740-6558759 Msa0440050:CDS 45.0%
CGCCATAAGGACTAATGGTT+CGG - chr3_3:6558903-6558922 None:intergenic 45.0%
TGGTTAATGAGCTCGCCATA+AGG - chr3_3:6558916-6558935 None:intergenic 45.0%
! GCTTCAACAAGATTTGCTCC+AGG + chr3_3:6559217-6559236 Msa0440050:intron 45.0%
TTGTTCCTTTGCTCCTAAGG+TGG + chr3_3:6559377-6559396 Msa0440050:intron 45.0%
!! TTGGTCTTGCTAGCACTATG+TGG - chr3_3:6559823-6559842 None:intergenic 45.0%
GTGCTAGCAAGACCAAGTTA+TGG + chr3_3:6559827-6559846 Msa0440050:intron 45.0%
AATGTGCAAGATCAACTCCC+TGG - chr3_3:6560216-6560235 None:intergenic 45.0%
CATGGGTCAGAGAACTTTCA+CGG - chr3_3:6560772-6560791 None:intergenic 45.0%
GGCAAGAGAAGAAGGCTTAT+TGG + chr3_3:6560931-6560950 Msa0440050:three_prime_UTR 45.0%
!! TTGAGCTCTCTTTGGGATTC+CGG + chr3_3:6560956-6560975 Msa0440050:three_prime_UTR 45.0%
TCAGATGGGATTTGAACTCC+CGG - chr3_3:6560978-6560997 None:intergenic 45.0%
!!! TTTTGGCTTCCCGGTTTTGT+TGG + chr3_3:6561486-6561505 Msa0440050:three_prime_UTR 45.0%
TGCCTAATGATCATCGATCG+AGG + chr3_3:6561553-6561572 Msa0440050:three_prime_UTR 45.0%
GATCATCGATCGAGGAAACT+AGG + chr3_3:6561561-6561580 Msa0440050:three_prime_UTR 45.0%
ATCTCAAAACAGAGAGCTCC+AGG - chr3_3:6561615-6561634 None:intergenic 45.0%
!!! CTCTCTGTTTTGAGATGCTC+TGG + chr3_3:6561619-6561638 Msa0440050:three_prime_UTR 45.0%
!!! TTTAAATTTTAATACAAAAA+TGG + chr3_3:6559152-6559171 Msa0440050:intron 5.0%
GTCTTTCCCCTCTGTGTCAA+AGG + chr3_3:6558800-6558819 Msa0440050:intron 50.0%
GAGCTCGCCATAAGGACTAA+TGG - chr3_3:6558908-6558927 None:intergenic 50.0%
CCAAACCACCTTAGGAGCAA+AGG - chr3_3:6559385-6559404 None:intergenic 50.0%
CCTTTGCTCCTAAGGTGGTT+TGG + chr3_3:6559382-6559401 Msa0440050:intron 50.0%
GTCTTGCTAGCACTATGTGG+CGG - chr3_3:6559820-6559839 None:intergenic 50.0%
GTGTGCCTGTGCAGAAAACA+CGG + chr3_3:6559968-6559987 Msa0440050:intron 50.0%
TGTGCCTGTGCAGAAAACAC+GGG + chr3_3:6559969-6559988 Msa0440050:intron 50.0%
! AAGTCCCGTGTTTTCTGCAC+AGG - chr3_3:6559976-6559995 None:intergenic 50.0%
ATCAAGGACTGAAGGAAGCC+AGG + chr3_3:6560195-6560214 Msa0440050:CDS 50.0%
TCAAGGACTGAAGGAAGCCA+GGG + chr3_3:6560196-6560215 Msa0440050:CDS 50.0%
GTCAGAGAACTTTCACGGAG+TGG - chr3_3:6560767-6560786 None:intergenic 50.0%
GATTGAATGATGGCTCCTGC+TGG + chr3_3:6560808-6560827 Msa0440050:intron 50.0%
!! GTTAGGTAGCTCTAACCAGC+AGG - chr3_3:6560826-6560845 None:intergenic 50.0%
!! TGAGCTCTCTTTGGGATTCC+GGG + chr3_3:6560957-6560976 Msa0440050:three_prime_UTR 50.0%
TGCAGTGCCCTGAATATTCG+AGG + chr3_3:6561339-6561358 Msa0440050:intron 50.0%
!!! GCAGCTGAATTTTGGCTTCC+CGG + chr3_3:6561477-6561496 Msa0440050:three_prime_UTR 50.0%
TGCTAGCACTATGTGGCGGT+GGG - chr3_3:6559816-6559835 None:intergenic 55.0%
TTGCTAGCACTATGTGGCGG+TGG - chr3_3:6559817-6559836 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 6558729 6561741 6558729 ID=Msa0440050;Name=Msa0440050
chr3_3 mRNA 6558729 6561741 6558729 ID=Msa0440050-mRNA-1;Parent=Msa0440050;Name=Msa0440050-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0.4|0.5|0.83|0.6|0.5|6|580|97
chr3_3 exon 6558729 6558761 6558729 ID=Msa0440050-mRNA-1:exon:1399;Parent=Msa0440050-mRNA-1
chr3_3 exon 6559982 6560014 6559982 ID=Msa0440050-mRNA-1:exon:1400;Parent=Msa0440050-mRNA-1
chr3_3 exon 6560155 6560285 6560155 ID=Msa0440050-mRNA-1:exon:1401;Parent=Msa0440050-mRNA-1
chr3_3 exon 6560809 6561024 6560809 ID=Msa0440050-mRNA-1:exon:1402;Parent=Msa0440050-mRNA-1
chr3_3 exon 6561176 6561238 6561176 ID=Msa0440050-mRNA-1:exon:1403;Parent=Msa0440050-mRNA-1
chr3_3 exon 6561344 6561741 6561344 ID=Msa0440050-mRNA-1:exon:1404;Parent=Msa0440050-mRNA-1
chr3_3 CDS 6558729 6558761 6558729 ID=Msa0440050-mRNA-1:cds;Parent=Msa0440050-mRNA-1
chr3_3 CDS 6559982 6560014 6559982 ID=Msa0440050-mRNA-1:cds;Parent=Msa0440050-mRNA-1
chr3_3 CDS 6560155 6560285 6560155 ID=Msa0440050-mRNA-1:cds;Parent=Msa0440050-mRNA-1
chr3_3 CDS 6560809 6560905 6560809 ID=Msa0440050-mRNA-1:cds;Parent=Msa0440050-mRNA-1
chr3_3 three_prime_UTR 6560906 6561024 6560906 ID=Msa0440050-mRNA-1:three_prime_utr;Parent=Msa0440050-mRNA-1
chr3_3 three_prime_UTR 6561176 6561238 6561176 ID=Msa0440050-mRNA-1:three_prime_utr;Parent=Msa0440050-mRNA-1
chr3_3 three_prime_UTR 6561344 6561741 6561344 ID=Msa0440050-mRNA-1:three_prime_utr;Parent=Msa0440050-mRNA-1
Gene Sequence

>Msa0440050

ATGGACTTCCCAGCACATCTTCTTCCTCTTCAGAAAACACGGGACTTGAGAAAAGAATGTGATGAGCTGGATGGCATAATTATCAAAACCATTGAGACAGCTTTATATCAAGGACTGAAGGAAGCCAGGGAGTTGATCTTGCACATTGAAAGGCATAATTTGTATCAGACATGCTCAGAGCTTTATACCAATGGTAAATTGAATGATGGCTCCTGCTGGTTAGAGCTACCTAACCATTCAGCTTCTCATGTCATGATAGGTCTTCACAAAGATGTTTTTGAGGAAAATGTTTGA
Protein sequence

>Msa0440050

MDFPAHLLPLQKTRDLRKECDELDGIIIKTIETALYQGLKEARELILHIERHNLYQTCSELYTNGKLNDGSCWLELPNHSASHVMIGLHKDVFEENV*