AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0443230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0443230 MtrunA17_Chr3g0081631 98.901 91 1 0 1 91 1 91 1.80e-64 189
Msa0443230 MtrunA17_Chr7g0255841 70.000 80 23 1 12 91 14 92 2.71e-36 118
Msa0443230 MtrunA17_Chr3g0141601 58.242 91 33 2 4 90 1 90 6.43e-32 107
Msa0443230 MtrunA17_Chr1g0155181 58.621 87 29 3 4 84 1 86 1.16e-30 103
Msa0443230 MtrunA17_Chr6g0449111 62.667 75 27 1 1 75 1 74 1.37e-30 103
Msa0443230 MtrunA17_Chr3g0144631 64.198 81 22 2 1 75 1 80 5.22e-30 102
Msa0443230 MtrunA17_Chr8g0373111 56.522 69 28 1 7 75 2 68 1.07e-23 85.5
Msa0443230 MtrunA17_Chr8g0373121 56.522 69 28 1 7 75 2 68 1.07e-23 85.5
Msa0443230 MtrunA17_Chr8g0373131 57.353 68 27 1 7 74 2 67 1.16e-23 85.5
Msa0443230 MtrunA17_Chr5g0404301 59.375 64 24 1 8 71 2 63 3.86e-23 84.0
Msa0443230 MtrunA17_Chr8g0373101 59.375 64 24 1 8 71 3 64 5.18e-23 84.0
Msa0443230 MtrunA17_Chr5g0404101 48.649 74 37 1 1 74 10 82 1.58e-21 80.9
Msa0443230 MtrunA17_Chr5g0436501 49.315 73 36 1 1 73 18 89 1.72e-17 74.3
Msa0443230 MtrunA17_Chr3g0141641 38.462 91 46 2 9 90 2 91 1.89e-16 67.8
Msa0443230 MtrunA17_Chr8g0364741 44.737 76 41 1 1 76 1 75 3.68e-16 68.2
Msa0443230 MtrunA17_Chr5g0429521 46.429 84 33 2 9 81 28 110 6.62e-16 70.5
Msa0443230 MtrunA17_Chr7g0241761 44.706 85 37 2 10 85 35 118 1.63e-15 69.3
Msa0443230 MtrunA17_Chr3g0104071 50.704 71 34 1 3 73 36 105 3.28e-15 68.6
Msa0443230 MtrunA17_Chr1g0194071 52.632 57 27 0 10 66 11 67 5.68e-15 63.9
Msa0443230 MtrunA17_Chr7g0270711 54.237 59 27 0 10 68 11 69 6.85e-15 63.9
Msa0443230 MtrunA17_Chr8g0365011 48.529 68 34 1 6 73 4 70 1.63e-14 66.6
Msa0443230 MtrunA17_Chr1g0162081 42.667 75 42 1 1 75 3 76 1.48e-13 63.9
Msa0443230 MtrunA17_Chr4g0062741 44.737 76 37 3 4 75 1 75 8.10e-13 62.0
Msa0443230 MtrunA17_Chr1g0210471 40.909 66 38 1 10 75 24 88 3.05e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0443230 AT4G39250.1 60.227 88 28 2 10 91 14 100 2.09e-34 114
Msa0443230 AT2G21650.1 61.798 89 26 2 10 91 14 101 2.43e-34 114
Msa0443230 AT1G75250.2 53.684 95 35 3 3 90 5 97 1.14e-27 96.7
Msa0443230 AT1G75250.1 60.811 74 28 1 3 76 5 77 2.32e-26 94.4
Msa0443230 AT1G19510.1 51.724 87 33 3 13 91 15 100 2.41e-22 83.6
Msa0443230 AT2G18328.1 63.492 63 22 1 13 75 14 75 4.87e-22 82.0
Msa0443230 AT4G36570.1 68.889 45 13 1 10 54 12 55 8.54e-17 68.2
Msa0443230 AT5G58900.1 52.000 75 32 2 2 73 23 96 1.28e-16 72.8
Msa0443230 AT5G05790.2 49.351 77 36 2 7 81 28 103 1.30e-16 72.4
Msa0443230 AT5G05790.1 49.351 77 36 2 7 81 28 103 1.30e-16 72.4
Msa0443230 AT2G38090.1 50.649 77 31 2 10 80 29 104 1.79e-16 72.4
Msa0443230 AT3G11280.2 48.438 64 32 1 10 73 33 95 8.48e-14 64.7
Msa0443230 AT3G11280.1 48.438 64 32 1 10 73 33 95 8.48e-14 64.7
Msa0443230 AT5G23650.1 47.222 72 37 1 4 75 7 77 1.56e-12 61.6
Msa0443230 AT5G08520.1 48.438 64 32 1 10 73 12 74 1.02e-11 59.3
Msa0443230 AT5G04760.1 39.706 68 39 1 6 73 2 67 2.71e-11 57.4

Find 57 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAATGTTCTTGTAATT+TGG 0.053146 3_3:-13874667 None:intergenic
CTCTAATTCTTCTTATAAAA+AGG 0.168485 3_3:+13874238 Msa0443230:five_prime_UTR
TGTCCCACTTTCTTGTCTTT+TGG 0.177759 3_3:+13875621 Msa0443230:three_prime_UTR
CTCCAATGTTCTTGTAATTT+GGG 0.244052 3_3:-13874666 None:intergenic
GACACTATGAACACCTTCTA+AGG 0.262540 3_3:+13874608 Msa0443230:CDS
TGTTGACACCATTTGGTTAC+TGG 0.305364 3_3:-13875445 None:intergenic
CAAGTTGGAATGAAAATCTT+TGG 0.319427 3_3:-13874190 None:intergenic
CATTTGGTTACTGGAGCTTC+AGG 0.328788 3_3:-13875436 None:intergenic
GGTACACATGTGGTAATAAA+TGG 0.334394 3_3:-13875701 None:intergenic
TGAACCTTGGTTTCAAGAAA+TGG 0.338544 3_3:+13874438 Msa0443230:exon
ATGAGCCCTTAAGACTTTGA+TGG 0.353444 3_3:-13874368 None:intergenic
TATTTGTTGTTGACACCATT+TGG 0.373580 3_3:-13875452 None:intergenic
CCACAACCATCAAAGTCTTA+AGG 0.373927 3_3:+13874362 Msa0443230:five_prime_UTR
CACAACCATCAAAGTCTTAA+GGG 0.386131 3_3:+13874363 Msa0443230:five_prime_UTR
TGGAAGTTAACAAAACTTGT+AGG 0.402986 3_3:-13874342 None:intergenic
TATGCCTAACATCCCTTAGA+AGG 0.404703 3_3:-13874621 None:intergenic
CAATGTCAGCATCTGGTTCA+TGG 0.413232 3_3:+13874464 Msa0443230:CDS
GATGTTAGGCATATTGAATC+AGG 0.424945 3_3:+13874631 Msa0443230:CDS
AAGAATAAGGCTTAAGGAAG+AGG 0.429086 3_3:-13874291 None:intergenic
TAGGCTATTCAAAATGTTGA+AGG 0.431834 3_3:-13874323 None:intergenic
TGTGTAAAGAATAAGGCTTA+AGG 0.455726 3_3:-13874297 None:intergenic
TAGAGGGCGTGTGGAAGGTG+TGG 0.460077 3_3:-13874220 None:intergenic
AGAGGGCGTGTGGAAGGTGT+GGG 0.464590 3_3:-13874219 None:intergenic
AACACCTTCTAAGGGATGTT+AGG 0.467379 3_3:+13874617 Msa0443230:CDS
GAAAGTGGGACATGGAATCA+TGG 0.467665 3_3:-13875610 None:intergenic
CCTTAAGACTTTGATGGTTG+TGG 0.470555 3_3:-13874362 None:intergenic
TAGTGTCTCTTAACATCCTC+AGG 0.475467 3_3:-13874592 None:intergenic
CCAAATTACAAGAACATTGG+AGG 0.482774 3_3:+13874667 Msa0443230:CDS
AGAATTAGAGGGCGTGTGGA+AGG 0.485502 3_3:-13874225 None:intergenic
ATGGCATCAATGTCAGCATC+TGG 0.486683 3_3:+13874457 Msa0443230:CDS
ACCAACAGCTTGAGCAACAT+TGG 0.499603 3_3:-13874557 None:intergenic
TTCCCAAATTACAAGAACAT+TGG 0.502964 3_3:+13874664 Msa0443230:CDS
AGGACAATAAAGCATTTGAA+AGG 0.506069 3_3:+13874494 Msa0443230:CDS
TGAAGAGAGGAAGCAATGCT+TGG 0.515866 3_3:-13874262 None:intergenic
TCCAATGTTGCTCAAGCTGT+TGG 0.520440 3_3:+13874556 Msa0443230:CDS
CTGAAGCTCCAGTAACCAAA+TGG 0.524122 3_3:+13875437 Msa0443230:exon
GGACAATAAAGCATTTGAAA+GGG 0.535274 3_3:+13874495 Msa0443230:CDS
GCAACATTGGACCAACGGTC+AGG 0.535547 3_3:-13874544 None:intergenic
GATGAAAAGAGATTATAGTA+AGG 0.535884 3_3:-13875722 None:intergenic
AGGTGTGGGGTAGAGCAAGT+TGG 0.539815 3_3:-13874205 None:intergenic
AAAGTGGGACATGGAATCAT+GGG 0.540922 3_3:-13875609 None:intergenic
AATGTTGCTCAAGCTGTTGG+TGG 0.546053 3_3:+13874559 Msa0443230:CDS
GTTGCTCAAGCTGTTGGTGG+TGG 0.554528 3_3:+13874562 Msa0443230:CDS
TTTCACTAATGCTACATCAC+TGG 0.559072 3_3:-13875562 None:intergenic
AAGAAGAATTAGAGGGCGTG+TGG 0.560374 3_3:-13874229 None:intergenic
GATGCCATTTCTTGAAACCA+AGG 0.568023 3_3:-13874442 None:intergenic
ATCTGGTTCATGGAGTGCTA+AGG 0.583163 3_3:+13874474 Msa0443230:CDS
GAGGGCGTGTGGAAGGTGTG+GGG 0.588905 3_3:-13874218 None:intergenic
ATAAAGACACACCTGACCGT+TGG 0.596639 3_3:+13874533 Msa0443230:CDS
TATTCCAAAAGACAAGAAAG+TGG 0.599303 3_3:-13875625 None:intergenic
ACACTATGAACACCTTCTAA+GGG 0.610400 3_3:+13874609 Msa0443230:CDS
AAAGACAAGAAAGTGGGACA+TGG 0.610999 3_3:-13875618 None:intergenic
ATTCCAAAAGACAAGAAAGT+GGG 0.631056 3_3:-13875624 None:intergenic
CTTGAGCAACATTGGACCAA+CGG 0.640722 3_3:-13874549 None:intergenic
GAACATTGGAGGCTATGATG+AGG 0.660409 3_3:+13874678 Msa0443230:CDS
TGGTGGTGGAAAGACTCCTG+AGG 0.678384 3_3:+13874576 Msa0443230:CDS
ATTATAGTAAGGTACACATG+TGG 0.707926 3_3:-13875711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTATAAACATATATT+AGG + chr3_3:13874729-13874748 Msa0443230:intron 10.0%
!!! TATTTATATGTACTAATTTT+TGG + chr3_3:13875125-13875144 Msa0443230:intron 10.0%
!!! ATTTATATGTACTAATTTTT+GGG + chr3_3:13875126-13875145 Msa0443230:intron 10.0%
!!! ATAAAGCTATAATTTTATTA+AGG - chr3_3:13875676-13875695 None:intergenic 10.0%
!!! TTTTCTTATAAACATATATT+AGG + chr3_3:13874729-13874748 Msa0443230:intron 10.0%
!!! TATTTATATGTACTAATTTT+TGG + chr3_3:13875125-13875144 Msa0443230:intron 10.0%
!!! ATTTATATGTACTAATTTTT+GGG + chr3_3:13875126-13875145 Msa0443230:intron 10.0%
!!! ATAAAGCTATAATTTTATTA+AGG - chr3_3:13875676-13875695 None:intergenic 10.0%
!!! TTTTTATAAGAAGAATTAGA+GGG - chr3_3:13874239-13874258 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_3:13875505-13875524 Msa0443230:three_prime_UTR 15.0%
!!! TTTTTATAAGAAGAATTAGA+GGG - chr3_3:13874239-13874258 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_3:13875505-13875524 Msa0443230:three_prime_UTR 15.0%
!!! CTTTTTATAAGAAGAATTAG+AGG - chr3_3:13874240-13874259 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+AGG + chr3_3:13874238-13874257 Msa0443230:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_3:13874406-13874425 Msa0443230:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_3:13874732-13874751 Msa0443230:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_3:13875063-13875082 None:intergenic 20.0%
!!! ACATGTCAAATTTTGAATAA+AGG - chr3_3:13875183-13875202 None:intergenic 20.0%
!!! AAGTTATAAGGTGTTTTAAA+AGG + chr3_3:13875271-13875290 Msa0443230:intron 20.0%
!!! ATTTTTAGAATCATTGGATT+AGG + chr3_3:13875511-13875530 Msa0443230:three_prime_UTR 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_3:13875671-13875690 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_3:13875672-13875691 None:intergenic 20.0%
!!! AACACATTATCTTCTTTTTT+TGG - chr3_3:13875784-13875803 None:intergenic 20.0%
!!! CTTTTTATAAGAAGAATTAG+AGG - chr3_3:13874240-13874259 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+AGG + chr3_3:13874238-13874257 Msa0443230:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_3:13874406-13874425 Msa0443230:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_3:13874732-13874751 Msa0443230:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_3:13875063-13875082 None:intergenic 20.0%
!!! ACATGTCAAATTTTGAATAA+AGG - chr3_3:13875183-13875202 None:intergenic 20.0%
!!! AAGTTATAAGGTGTTTTAAA+AGG + chr3_3:13875271-13875290 Msa0443230:intron 20.0%
!!! ATTTTTAGAATCATTGGATT+AGG + chr3_3:13875511-13875530 Msa0443230:three_prime_UTR 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_3:13875671-13875690 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_3:13875672-13875691 None:intergenic 20.0%
!!! AACACATTATCTTCTTTTTT+TGG - chr3_3:13875784-13875803 None:intergenic 20.0%
!!! AAGGTTTTGTGTAAAGAATA+AGG - chr3_3:13874307-13874326 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_3:13875024-13875043 Msa0443230:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_3:13875062-13875081 None:intergenic 25.0%
!! GAGTAAAAAAAGTGTAAGTT+GGG - chr3_3:13875241-13875260 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_3:13875725-13875744 None:intergenic 25.0%
!!! AAGGTTTTGTGTAAAGAATA+AGG - chr3_3:13874307-13874326 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_3:13875024-13875043 Msa0443230:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_3:13875062-13875081 None:intergenic 25.0%
!! GAGTAAAAAAAGTGTAAGTT+GGG - chr3_3:13875241-13875260 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_3:13875725-13875744 None:intergenic 25.0%
! CAAGTTGGAATGAAAATCTT+TGG - chr3_3:13874193-13874212 None:intergenic 30.0%
TGTGTAAAGAATAAGGCTTA+AGG - chr3_3:13874300-13874319 None:intergenic 30.0%
TAGGCTATTCAAAATGTTGA+AGG - chr3_3:13874326-13874345 None:intergenic 30.0%
TGGAAGTTAACAAAACTTGT+AGG - chr3_3:13874345-13874364 None:intergenic 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_3:13874494-13874513 Msa0443230:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_3:13874495-13874514 Msa0443230:CDS 30.0%
TTCCCAAATTACAAGAACAT+TGG + chr3_3:13874664-13874683 Msa0443230:CDS 30.0%
CTCCAATGTTCTTGTAATTT+GGG - chr3_3:13874669-13874688 None:intergenic 30.0%
ACGCTGAATAAGTATTATGA+AGG - chr3_3:13874778-13874797 None:intergenic 30.0%
AACTCATATCGATGATTTAC+TGG + chr3_3:13874801-13874820 Msa0443230:intron 30.0%
TCAAGCATAATTCATAAGAG+TGG + chr3_3:13875097-13875116 Msa0443230:intron 30.0%
!!! TACTAATTTTTGGGTCAAGT+GGG + chr3_3:13875135-13875154 Msa0443230:intron 30.0%
! GGAGTAAAAAAAGTGTAAGT+TGG - chr3_3:13875242-13875261 None:intergenic 30.0%
TCCATTCGTCTTAAGTTATA+AGG + chr3_3:13875259-13875278 Msa0443230:intron 30.0%
ACCTTATAACTTAAGACGAA+TGG - chr3_3:13875263-13875282 None:intergenic 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_3:13875455-13875474 None:intergenic 30.0%
ATTCCAAAAGACAAGAAAGT+GGG - chr3_3:13875627-13875646 None:intergenic 30.0%
TATTCCAAAAGACAAGAAAG+TGG - chr3_3:13875628-13875647 None:intergenic 30.0%
!!! CTTGTCTTTTGGAATATCAA+CGG + chr3_3:13875632-13875651 Msa0443230:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_3:13875714-13875733 None:intergenic 30.0%
! CAAGTTGGAATGAAAATCTT+TGG - chr3_3:13874193-13874212 None:intergenic 30.0%
TGTGTAAAGAATAAGGCTTA+AGG - chr3_3:13874300-13874319 None:intergenic 30.0%
TAGGCTATTCAAAATGTTGA+AGG - chr3_3:13874326-13874345 None:intergenic 30.0%
TGGAAGTTAACAAAACTTGT+AGG - chr3_3:13874345-13874364 None:intergenic 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_3:13874494-13874513 Msa0443230:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_3:13874495-13874514 Msa0443230:CDS 30.0%
TTCCCAAATTACAAGAACAT+TGG + chr3_3:13874664-13874683 Msa0443230:CDS 30.0%
CTCCAATGTTCTTGTAATTT+GGG - chr3_3:13874669-13874688 None:intergenic 30.0%
ACGCTGAATAAGTATTATGA+AGG - chr3_3:13874778-13874797 None:intergenic 30.0%
AACTCATATCGATGATTTAC+TGG + chr3_3:13874801-13874820 Msa0443230:intron 30.0%
TCAAGCATAATTCATAAGAG+TGG + chr3_3:13875097-13875116 Msa0443230:intron 30.0%
!!! TACTAATTTTTGGGTCAAGT+GGG + chr3_3:13875135-13875154 Msa0443230:intron 30.0%
! GGAGTAAAAAAAGTGTAAGT+TGG - chr3_3:13875242-13875261 None:intergenic 30.0%
TCCATTCGTCTTAAGTTATA+AGG + chr3_3:13875259-13875278 Msa0443230:intron 30.0%
ACCTTATAACTTAAGACGAA+TGG - chr3_3:13875263-13875282 None:intergenic 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_3:13875455-13875474 None:intergenic 30.0%
ATTCCAAAAGACAAGAAAGT+GGG - chr3_3:13875627-13875646 None:intergenic 30.0%
TATTCCAAAAGACAAGAAAG+TGG - chr3_3:13875628-13875647 None:intergenic 30.0%
!!! CTTGTCTTTTGGAATATCAA+CGG + chr3_3:13875632-13875651 Msa0443230:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_3:13875714-13875733 None:intergenic 30.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_3:13874294-13874313 None:intergenic 35.0%
CACAACCATCAAAGTCTTAA+GGG + chr3_3:13874363-13874382 Msa0443230:five_prime_UTR 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_3:13874425-13874444 Msa0443230:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_3:13874438-13874457 Msa0443230:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_3:13874609-13874628 Msa0443230:CDS 35.0%
GATGTTAGGCATATTGAATC+AGG + chr3_3:13874631-13874650 Msa0443230:CDS 35.0%
CCTCCAATGTTCTTGTAATT+TGG - chr3_3:13874670-13874689 None:intergenic 35.0%
CCAAATTACAAGAACATTGG+AGG + chr3_3:13874667-13874686 Msa0443230:CDS 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_3:13875053-13875072 None:intergenic 35.0%
!!! GTACTAATTTTTGGGTCAAG+TGG + chr3_3:13875134-13875153 Msa0443230:intron 35.0%
!!! ACTAATTTTTGGGTCAAGTG+GGG + chr3_3:13875136-13875155 Msa0443230:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_3:13875565-13875584 None:intergenic 35.0%
GGTACACATGTGGTAATAAA+TGG - chr3_3:13875704-13875723 None:intergenic 35.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_3:13874294-13874313 None:intergenic 35.0%
CACAACCATCAAAGTCTTAA+GGG + chr3_3:13874363-13874382 Msa0443230:five_prime_UTR 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_3:13874425-13874444 Msa0443230:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_3:13874438-13874457 Msa0443230:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_3:13874609-13874628 Msa0443230:CDS 35.0%
GATGTTAGGCATATTGAATC+AGG + chr3_3:13874631-13874650 Msa0443230:CDS 35.0%
CCTCCAATGTTCTTGTAATT+TGG - chr3_3:13874670-13874689 None:intergenic 35.0%
CCAAATTACAAGAACATTGG+AGG + chr3_3:13874667-13874686 Msa0443230:CDS 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_3:13875053-13875072 None:intergenic 35.0%
!!! GTACTAATTTTTGGGTCAAG+TGG + chr3_3:13875134-13875153 Msa0443230:intron 35.0%
!!! ACTAATTTTTGGGTCAAGTG+GGG + chr3_3:13875136-13875155 Msa0443230:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_3:13875565-13875584 None:intergenic 35.0%
GGTACACATGTGGTAATAAA+TGG - chr3_3:13875704-13875723 None:intergenic 35.0%
!! GAAGAGGTGATTTTGAAGAG+AGG - chr3_3:13874278-13874297 None:intergenic 40.0%
! CCTTAAGACTTTGATGGTTG+TGG - chr3_3:13874365-13874384 None:intergenic 40.0%
CCACAACCATCAAAGTCTTA+AGG + chr3_3:13874362-13874381 Msa0443230:five_prime_UTR 40.0%
ATGAGCCCTTAAGACTTTGA+TGG - chr3_3:13874371-13874390 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_3:13874445-13874464 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_3:13874595-13874614 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_3:13874608-13874627 Msa0443230:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_3:13874617-13874636 Msa0443230:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_3:13874624-13874643 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_3:13874686-13874705 Msa0443230:CDS 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_3:13875448-13875467 None:intergenic 40.0%
AAAGTGGGACATGGAATCAT+GGG - chr3_3:13875612-13875631 None:intergenic 40.0%
AAAGACAAGAAAGTGGGACA+TGG - chr3_3:13875621-13875640 None:intergenic 40.0%
! TGTCCCACTTTCTTGTCTTT+TGG + chr3_3:13875621-13875640 Msa0443230:three_prime_UTR 40.0%
!! GAAGAGGTGATTTTGAAGAG+AGG - chr3_3:13874278-13874297 None:intergenic 40.0%
! CCTTAAGACTTTGATGGTTG+TGG - chr3_3:13874365-13874384 None:intergenic 40.0%
CCACAACCATCAAAGTCTTA+AGG + chr3_3:13874362-13874381 Msa0443230:five_prime_UTR 40.0%
ATGAGCCCTTAAGACTTTGA+TGG - chr3_3:13874371-13874390 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_3:13874445-13874464 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_3:13874595-13874614 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_3:13874608-13874627 Msa0443230:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_3:13874617-13874636 Msa0443230:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_3:13874624-13874643 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_3:13874686-13874705 Msa0443230:CDS 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_3:13875448-13875467 None:intergenic 40.0%
AAAGTGGGACATGGAATCAT+GGG - chr3_3:13875612-13875631 None:intergenic 40.0%
AAAGACAAGAAAGTGGGACA+TGG - chr3_3:13875621-13875640 None:intergenic 40.0%
! TGTCCCACTTTCTTGTCTTT+TGG + chr3_3:13875621-13875640 Msa0443230:three_prime_UTR 40.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_3:13874232-13874251 None:intergenic 45.0%
TGAAGAGAGGAAGCAATGCT+TGG - chr3_3:13874265-13874284 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_3:13874457-13874476 Msa0443230:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_3:13874464-13874483 Msa0443230:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_3:13874474-13874493 Msa0443230:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_3:13874533-13874552 Msa0443230:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_3:13874552-13874571 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_3:13874556-13874575 Msa0443230:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_3:13874560-13874579 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_3:13874559-13874578 Msa0443230:CDS 45.0%
GAACATTGGAGGCTATGATG+AGG + chr3_3:13874678-13874697 Msa0443230:CDS 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_3:13875439-13875458 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_3:13875437-13875456 Msa0443230:exon 45.0%
GAAAGTGGGACATGGAATCA+TGG - chr3_3:13875613-13875632 None:intergenic 45.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_3:13874232-13874251 None:intergenic 45.0%
TGAAGAGAGGAAGCAATGCT+TGG - chr3_3:13874265-13874284 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_3:13874457-13874476 Msa0443230:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_3:13874464-13874483 Msa0443230:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_3:13874474-13874493 Msa0443230:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_3:13874533-13874552 Msa0443230:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_3:13874552-13874571 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_3:13874556-13874575 Msa0443230:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_3:13874560-13874579 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_3:13874559-13874578 Msa0443230:CDS 45.0%
GAACATTGGAGGCTATGATG+AGG + chr3_3:13874678-13874697 Msa0443230:CDS 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_3:13875439-13875458 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_3:13875437-13875456 Msa0443230:exon 45.0%
GAAAGTGGGACATGGAATCA+TGG - chr3_3:13875613-13875632 None:intergenic 45.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_3:13874228-13874247 None:intergenic 50.0%
AACCTGCAGGTGAAAGCAAG+AGG - chr3_3:13875410-13875429 None:intergenic 50.0%
! CTTCAGGTTTCTCAACCTGC+AGG - chr3_3:13875423-13875442 None:intergenic 50.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_3:13874228-13874247 None:intergenic 50.0%
AACCTGCAGGTGAAAGCAAG+AGG - chr3_3:13875410-13875429 None:intergenic 50.0%
! CTTCAGGTTTCTCAACCTGC+AGG - chr3_3:13875423-13875442 None:intergenic 50.0%
AGGTGTGGGGTAGAGCAAGT+TGG - chr3_3:13874208-13874227 None:intergenic 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_3:13874547-13874566 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_3:13874562-13874581 Msa0443230:CDS 55.0%
!! TGGTGGTGGAAAGACTCCTG+AGG + chr3_3:13874576-13874595 Msa0443230:CDS 55.0%
TGCCTCTTGCTTTCACCTGC+AGG + chr3_3:13875405-13875424 Msa0443230:intron 55.0%
AGGTGTGGGGTAGAGCAAGT+TGG - chr3_3:13874208-13874227 None:intergenic 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_3:13874547-13874566 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_3:13874562-13874581 Msa0443230:CDS 55.0%
!! TGGTGGTGGAAAGACTCCTG+AGG + chr3_3:13874576-13874595 Msa0443230:CDS 55.0%
TGCCTCTTGCTTTCACCTGC+AGG + chr3_3:13875405-13875424 Msa0443230:intron 55.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_3:13874222-13874241 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_3:13874223-13874242 None:intergenic 60.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_3:13874222-13874241 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_3:13874223-13874242 None:intergenic 60.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_3:13874221-13874240 None:intergenic 65.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_3:13874221-13874240 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 13874188 13875840 13874188 ID=Msa0443230;Name=Msa0443230
chr3_3 mRNA 13874188 13875840 13874188 ID=Msa0443230-mRNA-1;Parent=Msa0443230;Name=Msa0443230-mRNA-1;_AED=0.01;_eAED=0.01;_QI=269|1|1|1|1|1|2|389|91
chr3_3 exon 13874188 13874707 13874188 ID=Msa0443230-mRNA-1:exon:3007;Parent=Msa0443230-mRNA-1
chr3_3 exon 13875427 13875840 13875427 ID=Msa0443230-mRNA-1:exon:3008;Parent=Msa0443230-mRNA-1
chr3_3 five_prime_UTR 13874188 13874456 13874188 ID=Msa0443230-mRNA-1:five_prime_utr;Parent=Msa0443230-mRNA-1
chr3_3 CDS 13874457 13874707 13874457 ID=Msa0443230-mRNA-1:cds;Parent=Msa0443230-mRNA-1
chr3_3 CDS 13875427 13875451 13875427 ID=Msa0443230-mRNA-1:cds;Parent=Msa0443230-mRNA-1
chr3_3 three_prime_UTR 13875452 13875840 13875452 ID=Msa0443230-mRNA-1:three_prime_utr;Parent=Msa0443230-mRNA-1
Gene Sequence

>Msa0443230

ATGGCATCAATGTCAGCATCTGGTTCATGGAGTGCTAAGGACAATAAAGCATTTGAAAGGGCACTAGCTGTTTTTGATAAAGACACACCTGACCGTTGGTCCAATGTTGCTCAAGCTGTTGGTGGTGGAAAGACTCCTGAGGATGTTAAGAGACACTATGAACACCTTCTAAGGGATGTTAGGCATATTGAATCAGGTCATGTTGCATTCCCAAATTACAAGAACATTGGAGGCTATGATGAGGAAAAAAGGTTGAGAAACCTGAAGCTCCAGTAA
Protein sequence

>Msa0443230

MASMSASGSWSAKDNKAFERALAVFDKDTPDRWSNVAQAVGGGKTPEDVKRHYEHLLRDVRHIESGHVAFPNYKNIGGYDEEKRLRNLKLQ*