AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0490720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0490720 MtrunA17_Chr3g0081631 98.901 91 1 0 1 91 1 91 1.80e-64 189
Msa0490720 MtrunA17_Chr7g0255841 70.000 80 23 1 12 91 14 92 2.71e-36 118
Msa0490720 MtrunA17_Chr3g0141601 58.242 91 33 2 4 90 1 90 6.43e-32 107
Msa0490720 MtrunA17_Chr1g0155181 58.621 87 29 3 4 84 1 86 1.16e-30 103
Msa0490720 MtrunA17_Chr6g0449111 62.667 75 27 1 1 75 1 74 1.37e-30 103
Msa0490720 MtrunA17_Chr3g0144631 64.198 81 22 2 1 75 1 80 5.22e-30 102
Msa0490720 MtrunA17_Chr8g0373111 56.522 69 28 1 7 75 2 68 1.07e-23 85.5
Msa0490720 MtrunA17_Chr8g0373121 56.522 69 28 1 7 75 2 68 1.07e-23 85.5
Msa0490720 MtrunA17_Chr8g0373131 57.353 68 27 1 7 74 2 67 1.16e-23 85.5
Msa0490720 MtrunA17_Chr5g0404301 59.375 64 24 1 8 71 2 63 3.86e-23 84.0
Msa0490720 MtrunA17_Chr8g0373101 59.375 64 24 1 8 71 3 64 5.18e-23 84.0
Msa0490720 MtrunA17_Chr5g0404101 48.649 74 37 1 1 74 10 82 1.58e-21 80.9
Msa0490720 MtrunA17_Chr5g0436501 49.315 73 36 1 1 73 18 89 1.72e-17 74.3
Msa0490720 MtrunA17_Chr3g0141641 38.462 91 46 2 9 90 2 91 1.89e-16 67.8
Msa0490720 MtrunA17_Chr8g0364741 44.737 76 41 1 1 76 1 75 3.68e-16 68.2
Msa0490720 MtrunA17_Chr5g0429521 46.429 84 33 2 9 81 28 110 6.62e-16 70.5
Msa0490720 MtrunA17_Chr7g0241761 44.706 85 37 2 10 85 35 118 1.63e-15 69.3
Msa0490720 MtrunA17_Chr3g0104071 50.704 71 34 1 3 73 36 105 3.28e-15 68.6
Msa0490720 MtrunA17_Chr1g0194071 52.632 57 27 0 10 66 11 67 5.68e-15 63.9
Msa0490720 MtrunA17_Chr7g0270711 54.237 59 27 0 10 68 11 69 6.85e-15 63.9
Msa0490720 MtrunA17_Chr8g0365011 48.529 68 34 1 6 73 4 70 1.63e-14 66.6
Msa0490720 MtrunA17_Chr1g0162081 42.667 75 42 1 1 75 3 76 1.48e-13 63.9
Msa0490720 MtrunA17_Chr4g0062741 44.737 76 37 3 4 75 1 75 8.10e-13 62.0
Msa0490720 MtrunA17_Chr1g0210471 40.909 66 38 1 10 75 24 88 3.05e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0490720 AT4G39250.1 60.227 88 28 2 10 91 14 100 2.09e-34 114
Msa0490720 AT2G21650.1 61.798 89 26 2 10 91 14 101 2.43e-34 114
Msa0490720 AT1G75250.2 53.684 95 35 3 3 90 5 97 1.14e-27 96.7
Msa0490720 AT1G75250.1 60.811 74 28 1 3 76 5 77 2.32e-26 94.4
Msa0490720 AT1G19510.1 51.724 87 33 3 13 91 15 100 2.41e-22 83.6
Msa0490720 AT2G18328.1 63.492 63 22 1 13 75 14 75 4.87e-22 82.0
Msa0490720 AT4G36570.1 68.889 45 13 1 10 54 12 55 8.54e-17 68.2
Msa0490720 AT5G58900.1 52.000 75 32 2 2 73 23 96 1.28e-16 72.8
Msa0490720 AT5G05790.2 49.351 77 36 2 7 81 28 103 1.30e-16 72.4
Msa0490720 AT5G05790.1 49.351 77 36 2 7 81 28 103 1.30e-16 72.4
Msa0490720 AT2G38090.1 50.649 77 31 2 10 80 29 104 1.79e-16 72.4
Msa0490720 AT3G11280.2 48.438 64 32 1 10 73 33 95 8.48e-14 64.7
Msa0490720 AT3G11280.1 48.438 64 32 1 10 73 33 95 8.48e-14 64.7
Msa0490720 AT5G23650.1 47.222 72 37 1 4 75 7 77 1.56e-12 61.6
Msa0490720 AT5G08520.1 48.438 64 32 1 10 73 12 74 1.02e-11 59.3
Msa0490720 AT5G04760.1 39.706 68 39 1 6 73 2 67 2.71e-11 57.4

Find 57 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAATGTTCTTGTAATT+TGG 0.053146 3_4:-12388567 None:intergenic
CTCTAATTCTTCTTATAAAA+AGG 0.168485 3_4:+12388138 Msa0490720:five_prime_UTR
TGTCCCACTTTCTTGTCTTT+TGG 0.177759 3_4:+12389521 Msa0490720:three_prime_UTR
CTCCAATGTTCTTGTAATTT+GGG 0.244052 3_4:-12388566 None:intergenic
GACACTATGAACACCTTCTA+AGG 0.262540 3_4:+12388508 Msa0490720:CDS
TGTTGACACCATTTGGTTAC+TGG 0.305364 3_4:-12389345 None:intergenic
CAAGTTGGAATGAAAATCTT+TGG 0.319427 3_4:-12388090 None:intergenic
CATTTGGTTACTGGAGCTTC+AGG 0.328788 3_4:-12389336 None:intergenic
GGTACACATGTGGTAATAAA+TGG 0.334394 3_4:-12389601 None:intergenic
TGAACCTTGGTTTCAAGAAA+TGG 0.338544 3_4:+12388338 Msa0490720:exon
ATGAGCCCTTAAGACTTTGA+TGG 0.353444 3_4:-12388268 None:intergenic
TATTTGTTGTTGACACCATT+TGG 0.373580 3_4:-12389352 None:intergenic
CCACAACCATCAAAGTCTTA+AGG 0.373927 3_4:+12388262 Msa0490720:five_prime_UTR
CACAACCATCAAAGTCTTAA+GGG 0.386131 3_4:+12388263 Msa0490720:five_prime_UTR
TGGAAGTTAACAAAACTTGT+AGG 0.402986 3_4:-12388242 None:intergenic
TATGCCTAACATCCCTTAGA+AGG 0.404703 3_4:-12388521 None:intergenic
CAATGTCAGCATCTGGTTCA+TGG 0.413232 3_4:+12388364 Msa0490720:CDS
GATGTTAGGCATATTGAATC+AGG 0.424945 3_4:+12388531 Msa0490720:CDS
AAGAATAAGGCTTAAGGAAG+AGG 0.429086 3_4:-12388191 None:intergenic
TAGGCTATTCAAAATGTTGA+AGG 0.431834 3_4:-12388223 None:intergenic
TGTGTAAAGAATAAGGCTTA+AGG 0.455726 3_4:-12388197 None:intergenic
TAGAGGGCGTGTGGAAGGTG+TGG 0.460077 3_4:-12388120 None:intergenic
AGAGGGCGTGTGGAAGGTGT+GGG 0.464590 3_4:-12388119 None:intergenic
AACACCTTCTAAGGGATGTT+AGG 0.467379 3_4:+12388517 Msa0490720:CDS
GAAAGTGGGACATGGAATCA+TGG 0.467665 3_4:-12389510 None:intergenic
CCTTAAGACTTTGATGGTTG+TGG 0.470555 3_4:-12388262 None:intergenic
TAGTGTCTCTTAACATCCTC+AGG 0.475467 3_4:-12388492 None:intergenic
CCAAATTACAAGAACATTGG+AGG 0.482774 3_4:+12388567 Msa0490720:CDS
AGAATTAGAGGGCGTGTGGA+AGG 0.485502 3_4:-12388125 None:intergenic
ATGGCATCAATGTCAGCATC+TGG 0.486683 3_4:+12388357 Msa0490720:CDS
ACCAACAGCTTGAGCAACAT+TGG 0.499603 3_4:-12388457 None:intergenic
TTCCCAAATTACAAGAACAT+TGG 0.502964 3_4:+12388564 Msa0490720:CDS
AGGACAATAAAGCATTTGAA+AGG 0.506069 3_4:+12388394 Msa0490720:CDS
TGAAGAGAGGAAGCAATGCT+TGG 0.515866 3_4:-12388162 None:intergenic
TCCAATGTTGCTCAAGCTGT+TGG 0.520440 3_4:+12388456 Msa0490720:CDS
CTGAAGCTCCAGTAACCAAA+TGG 0.524122 3_4:+12389337 Msa0490720:exon
GGACAATAAAGCATTTGAAA+GGG 0.535274 3_4:+12388395 Msa0490720:CDS
GCAACATTGGACCAACGGTC+AGG 0.535547 3_4:-12388444 None:intergenic
GATGAAAAGAGATTATAGTA+AGG 0.535884 3_4:-12389622 None:intergenic
AGGTGTGGGGTAGAGCAAGT+TGG 0.539815 3_4:-12388105 None:intergenic
AAAGTGGGACATGGAATCAT+GGG 0.540922 3_4:-12389509 None:intergenic
AATGTTGCTCAAGCTGTTGG+TGG 0.546053 3_4:+12388459 Msa0490720:CDS
GTTGCTCAAGCTGTTGGTGG+TGG 0.554528 3_4:+12388462 Msa0490720:CDS
TTTCACTAATGCTACATCAC+TGG 0.559072 3_4:-12389462 None:intergenic
AAGAAGAATTAGAGGGCGTG+TGG 0.560374 3_4:-12388129 None:intergenic
GATGCCATTTCTTGAAACCA+AGG 0.568023 3_4:-12388342 None:intergenic
ATCTGGTTCATGGAGTGCTA+AGG 0.583163 3_4:+12388374 Msa0490720:CDS
GAGGGCGTGTGGAAGGTGTG+GGG 0.588905 3_4:-12388118 None:intergenic
ATAAAGACACACCTGACCGT+TGG 0.596639 3_4:+12388433 Msa0490720:CDS
TATTCCAAAAGACAAGAAAG+TGG 0.599303 3_4:-12389525 None:intergenic
ACACTATGAACACCTTCTAA+GGG 0.610400 3_4:+12388509 Msa0490720:CDS
AAAGACAAGAAAGTGGGACA+TGG 0.610999 3_4:-12389518 None:intergenic
ATTCCAAAAGACAAGAAAGT+GGG 0.631056 3_4:-12389524 None:intergenic
CTTGAGCAACATTGGACCAA+CGG 0.640722 3_4:-12388449 None:intergenic
GAACATTGGAGGCTATGATG+AGG 0.660409 3_4:+12388578 Msa0490720:CDS
TGGTGGTGGAAAGACTCCTG+AGG 0.678384 3_4:+12388476 Msa0490720:CDS
ATTATAGTAAGGTACACATG+TGG 0.707926 3_4:-12389611 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTATAAACATATATT+AGG + chr3_4:12388629-12388648 Msa0490720:intron 10.0%
!!! TATTTATATGTACTAATTTT+TGG + chr3_4:12389025-12389044 Msa0490720:intron 10.0%
!!! ATTTATATGTACTAATTTTT+GGG + chr3_4:12389026-12389045 Msa0490720:intron 10.0%
!!! ATAAAGCTATAATTTTATTA+AGG - chr3_4:12389576-12389595 None:intergenic 10.0%
!!! TTTTCTTATAAACATATATT+AGG + chr3_4:12388629-12388648 Msa0490720:intron 10.0%
!!! TATTTATATGTACTAATTTT+TGG + chr3_4:12389025-12389044 Msa0490720:intron 10.0%
!!! ATTTATATGTACTAATTTTT+GGG + chr3_4:12389026-12389045 Msa0490720:intron 10.0%
!!! ATAAAGCTATAATTTTATTA+AGG - chr3_4:12389576-12389595 None:intergenic 10.0%
!!! TTTTTATAAGAAGAATTAGA+GGG - chr3_4:12388139-12388158 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_4:12389405-12389424 Msa0490720:three_prime_UTR 15.0%
!!! TTTTTATAAGAAGAATTAGA+GGG - chr3_4:12388139-12388158 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_4:12389405-12389424 Msa0490720:three_prime_UTR 15.0%
!!! CTTTTTATAAGAAGAATTAG+AGG - chr3_4:12388140-12388159 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+AGG + chr3_4:12388138-12388157 Msa0490720:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_4:12388306-12388325 Msa0490720:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_4:12388632-12388651 Msa0490720:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_4:12388963-12388982 None:intergenic 20.0%
!!! ACATGTCAAATTTTGAATAA+AGG - chr3_4:12389083-12389102 None:intergenic 20.0%
!!! AAGTTATAAGGTGTTTTAAA+AGG + chr3_4:12389170-12389189 Msa0490720:intron 20.0%
!!! ATTTTTAGAATCATTGGATT+AGG + chr3_4:12389411-12389430 Msa0490720:three_prime_UTR 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_4:12389571-12389590 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_4:12389572-12389591 None:intergenic 20.0%
!!! AACACATTATCTTCTTTTTT+TGG - chr3_4:12389684-12389703 None:intergenic 20.0%
!!! CTTTTTATAAGAAGAATTAG+AGG - chr3_4:12388140-12388159 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+AGG + chr3_4:12388138-12388157 Msa0490720:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_4:12388306-12388325 Msa0490720:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_4:12388632-12388651 Msa0490720:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_4:12388963-12388982 None:intergenic 20.0%
!!! ACATGTCAAATTTTGAATAA+AGG - chr3_4:12389083-12389102 None:intergenic 20.0%
!!! AAGTTATAAGGTGTTTTAAA+AGG + chr3_4:12389170-12389189 Msa0490720:intron 20.0%
!!! ATTTTTAGAATCATTGGATT+AGG + chr3_4:12389411-12389430 Msa0490720:three_prime_UTR 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_4:12389571-12389590 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_4:12389572-12389591 None:intergenic 20.0%
!!! AACACATTATCTTCTTTTTT+TGG - chr3_4:12389684-12389703 None:intergenic 20.0%
!!! AAGGTTTTGTGTAAAGAATA+AGG - chr3_4:12388207-12388226 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_4:12388924-12388943 Msa0490720:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_4:12388962-12388981 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_4:12389625-12389644 None:intergenic 25.0%
!!! AAGGTTTTGTGTAAAGAATA+AGG - chr3_4:12388207-12388226 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_4:12388924-12388943 Msa0490720:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_4:12388962-12388981 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_4:12389625-12389644 None:intergenic 25.0%
! CAAGTTGGAATGAAAATCTT+TGG - chr3_4:12388093-12388112 None:intergenic 30.0%
TGTGTAAAGAATAAGGCTTA+AGG - chr3_4:12388200-12388219 None:intergenic 30.0%
TAGGCTATTCAAAATGTTGA+AGG - chr3_4:12388226-12388245 None:intergenic 30.0%
TGGAAGTTAACAAAACTTGT+AGG - chr3_4:12388245-12388264 None:intergenic 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_4:12388394-12388413 Msa0490720:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_4:12388395-12388414 Msa0490720:CDS 30.0%
TTCCCAAATTACAAGAACAT+TGG + chr3_4:12388564-12388583 Msa0490720:CDS 30.0%
CTCCAATGTTCTTGTAATTT+GGG - chr3_4:12388569-12388588 None:intergenic 30.0%
ACGCTGAATAAGTATTATGA+AGG - chr3_4:12388678-12388697 None:intergenic 30.0%
AACTCATATCGATGATTTAC+TGG + chr3_4:12388701-12388720 Msa0490720:intron 30.0%
TCAAGCATAATTCATAAGAG+TGG + chr3_4:12388997-12389016 Msa0490720:intron 30.0%
!!! TACTAATTTTTGGGTCAAGT+GGG + chr3_4:12389035-12389054 Msa0490720:intron 30.0%
! GGAGTAAAAAAGTGTAAGTT+GGG - chr3_4:12389141-12389160 None:intergenic 30.0%
! TGGAGTAAAAAAGTGTAAGT+TGG - chr3_4:12389142-12389161 None:intergenic 30.0%
TCCATTCGTCTTAAGTTATA+AGG + chr3_4:12389158-12389177 Msa0490720:intron 30.0%
ACCTTATAACTTAAGACGAA+TGG - chr3_4:12389162-12389181 None:intergenic 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_4:12389355-12389374 None:intergenic 30.0%
ATTCCAAAAGACAAGAAAGT+GGG - chr3_4:12389527-12389546 None:intergenic 30.0%
TATTCCAAAAGACAAGAAAG+TGG - chr3_4:12389528-12389547 None:intergenic 30.0%
!!! CTTGTCTTTTGGAATATCAA+CGG + chr3_4:12389532-12389551 Msa0490720:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_4:12389614-12389633 None:intergenic 30.0%
! CAAGTTGGAATGAAAATCTT+TGG - chr3_4:12388093-12388112 None:intergenic 30.0%
TGTGTAAAGAATAAGGCTTA+AGG - chr3_4:12388200-12388219 None:intergenic 30.0%
TAGGCTATTCAAAATGTTGA+AGG - chr3_4:12388226-12388245 None:intergenic 30.0%
TGGAAGTTAACAAAACTTGT+AGG - chr3_4:12388245-12388264 None:intergenic 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_4:12388394-12388413 Msa0490720:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_4:12388395-12388414 Msa0490720:CDS 30.0%
TTCCCAAATTACAAGAACAT+TGG + chr3_4:12388564-12388583 Msa0490720:CDS 30.0%
CTCCAATGTTCTTGTAATTT+GGG - chr3_4:12388569-12388588 None:intergenic 30.0%
ACGCTGAATAAGTATTATGA+AGG - chr3_4:12388678-12388697 None:intergenic 30.0%
AACTCATATCGATGATTTAC+TGG + chr3_4:12388701-12388720 Msa0490720:intron 30.0%
TCAAGCATAATTCATAAGAG+TGG + chr3_4:12388997-12389016 Msa0490720:intron 30.0%
!!! TACTAATTTTTGGGTCAAGT+GGG + chr3_4:12389035-12389054 Msa0490720:intron 30.0%
! GGAGTAAAAAAGTGTAAGTT+GGG - chr3_4:12389141-12389160 None:intergenic 30.0%
! TGGAGTAAAAAAGTGTAAGT+TGG - chr3_4:12389142-12389161 None:intergenic 30.0%
TCCATTCGTCTTAAGTTATA+AGG + chr3_4:12389158-12389177 Msa0490720:intron 30.0%
ACCTTATAACTTAAGACGAA+TGG - chr3_4:12389162-12389181 None:intergenic 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_4:12389355-12389374 None:intergenic 30.0%
ATTCCAAAAGACAAGAAAGT+GGG - chr3_4:12389527-12389546 None:intergenic 30.0%
TATTCCAAAAGACAAGAAAG+TGG - chr3_4:12389528-12389547 None:intergenic 30.0%
!!! CTTGTCTTTTGGAATATCAA+CGG + chr3_4:12389532-12389551 Msa0490720:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_4:12389614-12389633 None:intergenic 30.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_4:12388194-12388213 None:intergenic 35.0%
CACAACCATCAAAGTCTTAA+GGG + chr3_4:12388263-12388282 Msa0490720:five_prime_UTR 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_4:12388325-12388344 Msa0490720:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_4:12388338-12388357 Msa0490720:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_4:12388509-12388528 Msa0490720:CDS 35.0%
GATGTTAGGCATATTGAATC+AGG + chr3_4:12388531-12388550 Msa0490720:CDS 35.0%
CCTCCAATGTTCTTGTAATT+TGG - chr3_4:12388570-12388589 None:intergenic 35.0%
CCAAATTACAAGAACATTGG+AGG + chr3_4:12388567-12388586 Msa0490720:CDS 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_4:12388953-12388972 None:intergenic 35.0%
!!! GTACTAATTTTTGGGTCAAG+TGG + chr3_4:12389034-12389053 Msa0490720:intron 35.0%
!!! ACTAATTTTTGGGTCAAGTG+GGG + chr3_4:12389036-12389055 Msa0490720:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_4:12389465-12389484 None:intergenic 35.0%
GGTACACATGTGGTAATAAA+TGG - chr3_4:12389604-12389623 None:intergenic 35.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_4:12388194-12388213 None:intergenic 35.0%
CACAACCATCAAAGTCTTAA+GGG + chr3_4:12388263-12388282 Msa0490720:five_prime_UTR 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_4:12388325-12388344 Msa0490720:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_4:12388338-12388357 Msa0490720:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_4:12388509-12388528 Msa0490720:CDS 35.0%
GATGTTAGGCATATTGAATC+AGG + chr3_4:12388531-12388550 Msa0490720:CDS 35.0%
CCTCCAATGTTCTTGTAATT+TGG - chr3_4:12388570-12388589 None:intergenic 35.0%
CCAAATTACAAGAACATTGG+AGG + chr3_4:12388567-12388586 Msa0490720:CDS 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_4:12388953-12388972 None:intergenic 35.0%
!!! GTACTAATTTTTGGGTCAAG+TGG + chr3_4:12389034-12389053 Msa0490720:intron 35.0%
!!! ACTAATTTTTGGGTCAAGTG+GGG + chr3_4:12389036-12389055 Msa0490720:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_4:12389465-12389484 None:intergenic 35.0%
GGTACACATGTGGTAATAAA+TGG - chr3_4:12389604-12389623 None:intergenic 35.0%
!! GAAGAGGTGATTTTGAAGAG+AGG - chr3_4:12388178-12388197 None:intergenic 40.0%
! CCTTAAGACTTTGATGGTTG+TGG - chr3_4:12388265-12388284 None:intergenic 40.0%
CCACAACCATCAAAGTCTTA+AGG + chr3_4:12388262-12388281 Msa0490720:five_prime_UTR 40.0%
ATGAGCCCTTAAGACTTTGA+TGG - chr3_4:12388271-12388290 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_4:12388345-12388364 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_4:12388495-12388514 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_4:12388508-12388527 Msa0490720:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_4:12388517-12388536 Msa0490720:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_4:12388524-12388543 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_4:12388586-12388605 Msa0490720:CDS 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_4:12389348-12389367 None:intergenic 40.0%
AAAGTGGGACATGGAATCAT+GGG - chr3_4:12389512-12389531 None:intergenic 40.0%
AAAGACAAGAAAGTGGGACA+TGG - chr3_4:12389521-12389540 None:intergenic 40.0%
! TGTCCCACTTTCTTGTCTTT+TGG + chr3_4:12389521-12389540 Msa0490720:three_prime_UTR 40.0%
!! GAAGAGGTGATTTTGAAGAG+AGG - chr3_4:12388178-12388197 None:intergenic 40.0%
! CCTTAAGACTTTGATGGTTG+TGG - chr3_4:12388265-12388284 None:intergenic 40.0%
CCACAACCATCAAAGTCTTA+AGG + chr3_4:12388262-12388281 Msa0490720:five_prime_UTR 40.0%
ATGAGCCCTTAAGACTTTGA+TGG - chr3_4:12388271-12388290 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_4:12388345-12388364 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_4:12388495-12388514 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_4:12388508-12388527 Msa0490720:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_4:12388517-12388536 Msa0490720:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_4:12388524-12388543 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_4:12388586-12388605 Msa0490720:CDS 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_4:12389348-12389367 None:intergenic 40.0%
AAAGTGGGACATGGAATCAT+GGG - chr3_4:12389512-12389531 None:intergenic 40.0%
AAAGACAAGAAAGTGGGACA+TGG - chr3_4:12389521-12389540 None:intergenic 40.0%
! TGTCCCACTTTCTTGTCTTT+TGG + chr3_4:12389521-12389540 Msa0490720:three_prime_UTR 40.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_4:12388132-12388151 None:intergenic 45.0%
TGAAGAGAGGAAGCAATGCT+TGG - chr3_4:12388165-12388184 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_4:12388357-12388376 Msa0490720:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_4:12388364-12388383 Msa0490720:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_4:12388374-12388393 Msa0490720:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_4:12388433-12388452 Msa0490720:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_4:12388452-12388471 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_4:12388456-12388475 Msa0490720:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_4:12388460-12388479 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_4:12388459-12388478 Msa0490720:CDS 45.0%
GAACATTGGAGGCTATGATG+AGG + chr3_4:12388578-12388597 Msa0490720:CDS 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_4:12389339-12389358 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_4:12389337-12389356 Msa0490720:exon 45.0%
GAAAGTGGGACATGGAATCA+TGG - chr3_4:12389513-12389532 None:intergenic 45.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_4:12388132-12388151 None:intergenic 45.0%
TGAAGAGAGGAAGCAATGCT+TGG - chr3_4:12388165-12388184 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_4:12388357-12388376 Msa0490720:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_4:12388364-12388383 Msa0490720:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_4:12388374-12388393 Msa0490720:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_4:12388433-12388452 Msa0490720:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_4:12388452-12388471 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_4:12388456-12388475 Msa0490720:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_4:12388460-12388479 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_4:12388459-12388478 Msa0490720:CDS 45.0%
GAACATTGGAGGCTATGATG+AGG + chr3_4:12388578-12388597 Msa0490720:CDS 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_4:12389339-12389358 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_4:12389337-12389356 Msa0490720:exon 45.0%
GAAAGTGGGACATGGAATCA+TGG - chr3_4:12389513-12389532 None:intergenic 45.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_4:12388128-12388147 None:intergenic 50.0%
AACCTGCAGGTGAAAGCAAG+AGG - chr3_4:12389310-12389329 None:intergenic 50.0%
! CTTCAGGTTTCTCAACCTGC+AGG - chr3_4:12389323-12389342 None:intergenic 50.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_4:12388128-12388147 None:intergenic 50.0%
AACCTGCAGGTGAAAGCAAG+AGG - chr3_4:12389310-12389329 None:intergenic 50.0%
! CTTCAGGTTTCTCAACCTGC+AGG - chr3_4:12389323-12389342 None:intergenic 50.0%
AGGTGTGGGGTAGAGCAAGT+TGG - chr3_4:12388108-12388127 None:intergenic 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_4:12388447-12388466 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_4:12388462-12388481 Msa0490720:CDS 55.0%
!! TGGTGGTGGAAAGACTCCTG+AGG + chr3_4:12388476-12388495 Msa0490720:CDS 55.0%
TGCCTCTTGCTTTCACCTGC+AGG + chr3_4:12389305-12389324 Msa0490720:intron 55.0%
AGGTGTGGGGTAGAGCAAGT+TGG - chr3_4:12388108-12388127 None:intergenic 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_4:12388447-12388466 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_4:12388462-12388481 Msa0490720:CDS 55.0%
!! TGGTGGTGGAAAGACTCCTG+AGG + chr3_4:12388476-12388495 Msa0490720:CDS 55.0%
TGCCTCTTGCTTTCACCTGC+AGG + chr3_4:12389305-12389324 Msa0490720:intron 55.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_4:12388122-12388141 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_4:12388123-12388142 None:intergenic 60.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_4:12388122-12388141 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_4:12388123-12388142 None:intergenic 60.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_4:12388121-12388140 None:intergenic 65.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_4:12388121-12388140 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 12388088 12389740 12388088 ID=Msa0490720;Name=Msa0490720
chr3_4 mRNA 12388088 12389740 12388088 ID=Msa0490720-mRNA-1;Parent=Msa0490720;Name=Msa0490720-mRNA-1;_AED=0.01;_eAED=0.01;_QI=269|1|1|1|1|1|2|389|91
chr3_4 exon 12388088 12388607 12388088 ID=Msa0490720-mRNA-1:exon:3828;Parent=Msa0490720-mRNA-1
chr3_4 exon 12389327 12389740 12389327 ID=Msa0490720-mRNA-1:exon:3829;Parent=Msa0490720-mRNA-1
chr3_4 five_prime_UTR 12388088 12388356 12388088 ID=Msa0490720-mRNA-1:five_prime_utr;Parent=Msa0490720-mRNA-1
chr3_4 CDS 12388357 12388607 12388357 ID=Msa0490720-mRNA-1:cds;Parent=Msa0490720-mRNA-1
chr3_4 CDS 12389327 12389351 12389327 ID=Msa0490720-mRNA-1:cds;Parent=Msa0490720-mRNA-1
chr3_4 three_prime_UTR 12389352 12389740 12389352 ID=Msa0490720-mRNA-1:three_prime_utr;Parent=Msa0490720-mRNA-1
Gene Sequence

>Msa0490720

ATGGCATCAATGTCAGCATCTGGTTCATGGAGTGCTAAGGACAATAAAGCATTTGAAAGGGCACTAGCTGTTTTTGATAAAGACACACCTGACCGTTGGTCCAATGTTGCTCAAGCTGTTGGTGGTGGAAAGACTCCTGAGGATGTTAAGAGACACTATGAACACCTTCTAAGGGATGTTAGGCATATTGAATCAGGTCATGTTGCATTCCCAAATTACAAGAACATTGGAGGCTATGATGAGGAAAAAAGGTTGAGAAACCTGAAGCTCCAGTAA
Protein sequence

>Msa0490720

MASMSASGSWSAKDNKAFERALAVFDKDTPDRWSNVAQAVGGGKTPEDVKRHYEHLLRDVRHIESGHVAFPNYKNIGGYDEEKRLRNLKLQ*