Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0444140 | KEH33596.1 | 90.164 | 61 | 6 | 0 | 1 | 61 | 53 | 113 | 4.82e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0444140 | G7ZWU3 | 90.164 | 61 | 6 | 0 | 1 | 61 | 53 | 113 | 2.30e-32 | 117 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0444140 | Msa1015450 | 0.851894 | 6.592338e-61 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0444140 | MtrunA17_Chr8g0356411 | 91.803 | 61 | 5 | 0 | 1 | 61 | 1 | 61 | 6.29e-36 | 115 |
| Msa0444140 | MtrunA17_Chr5g0419101 | 89.655 | 58 | 6 | 0 | 1 | 58 | 45 | 102 | 3.85e-33 | 109 |
| Msa0444140 | MtrunA17_Chr2g0309021 | 87.931 | 58 | 7 | 0 | 1 | 58 | 469 | 526 | 4.80e-29 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTGTATCATTGGGAAATA+AGG | 0.351637 | 3_3:+16979605 | None:intergenic |
| TTATACCTGTTGTGTATCAT+TGG | 0.404371 | 3_3:+16979596 | None:intergenic |
| GATGATCTAGATATGTCATT+AGG | 0.423580 | 3_3:+16978925 | None:intergenic |
| TGCAACTATGGAGGCTCAAA+TGG | 0.437214 | 3_3:-16979691 | None:intergenic |
| GCTCAAATGGCAGCACTTGT+TGG | 0.439947 | 3_3:-16979678 | Msa0444140:CDS |
| ATTTCCCAATGATACACAAC+AGG | 0.483592 | 3_3:-16979601 | Msa0444140:intron |
| AATAAGGCAGCAGCTAACTC+TGG | 0.498255 | 3_3:+16979621 | None:intergenic |
| TATACCTGTTGTGTATCATT+GGG | 0.541600 | 3_3:+16979597 | None:intergenic |
| CTAGATATGTCATTAGGTGT+TGG | 0.547570 | 3_3:+16978931 | None:intergenic |
| TACATTAAAGCGAAGGAAGG+TGG | 0.589028 | 3_3:-16979654 | Msa0444140:CDS |
| TGTTGGCTACATTAAAGCGA+AGG | 0.611559 | 3_3:-16979661 | Msa0444140:CDS |
| TCAGATGAAAGCAACGCACA+TGG | 0.615460 | 3_3:-16978904 | Msa0444140:CDS |
| GGCTACATTAAAGCGAAGGA+AGG | 0.617737 | 3_3:-16979657 | Msa0444140:CDS |
| AAGGCAGCAGCTAACTCTGG+AGG | 0.673734 | 3_3:+16979624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATAAATGAAAGTTTTAA+GGG | - | chr3_3:16979235-16979254 | Msa0444140:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | - | chr3_3:16979355-16979374 | Msa0444140:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | - | chr3_3:16979479-16979498 | Msa0444140:intron | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | - | chr3_3:16979595-16979614 | Msa0444140:intron | 15.0% |
| !!! | AGAATAAATGAAAGTTTTAA+GGG | - | chr3_3:16979235-16979254 | Msa0444140:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | - | chr3_3:16979355-16979374 | Msa0444140:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | - | chr3_3:16979479-16979498 | Msa0444140:intron | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | - | chr3_3:16979595-16979614 | Msa0444140:intron | 15.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | + | chr3_3:16979118-16979137 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | + | chr3_3:16979119-16979138 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | - | chr3_3:16979234-16979253 | Msa0444140:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | + | chr3_3:16979268-16979287 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | - | chr3_3:16979532-16979551 | Msa0444140:intron | 20.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | + | chr3_3:16979118-16979137 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | + | chr3_3:16979119-16979138 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | - | chr3_3:16979234-16979253 | Msa0444140:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | + | chr3_3:16979268-16979287 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | - | chr3_3:16979532-16979551 | Msa0444140:intron | 20.0% |
| ! | TATATATTTAGCAATGAGGA+AGG | + | chr3_3:16979061-16979080 | None:intergenic | 25.0% |
| ! | TCTCTATATATTTAGCAATG+AGG | + | chr3_3:16979065-16979084 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | - | chr3_3:16979241-16979260 | Msa0444140:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | - | chr3_3:16979371-16979390 | Msa0444140:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | - | chr3_3:16979372-16979391 | Msa0444140:intron | 25.0% |
| ! | TATATATTTAGCAATGAGGA+AGG | + | chr3_3:16979061-16979080 | None:intergenic | 25.0% |
| ! | TCTCTATATATTTAGCAATG+AGG | + | chr3_3:16979065-16979084 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | - | chr3_3:16979241-16979260 | Msa0444140:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | - | chr3_3:16979371-16979390 | Msa0444140:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | - | chr3_3:16979372-16979391 | Msa0444140:intron | 25.0% |
| TTGTGTATCATTGGGAAATA+AGG | + | chr3_3:16978970-16978989 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | + | chr3_3:16978978-16978997 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | + | chr3_3:16978979-16978998 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | + | chr3_3:16979110-16979129 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | + | chr3_3:16979145-16979164 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | - | chr3_3:16979182-16979201 | Msa0444140:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | + | chr3_3:16979650-16979669 | None:intergenic | 30.0% | |
| TTGTGTATCATTGGGAAATA+AGG | + | chr3_3:16978970-16978989 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | + | chr3_3:16978978-16978997 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | + | chr3_3:16978979-16978998 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | + | chr3_3:16979110-16979129 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | + | chr3_3:16979145-16979164 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | - | chr3_3:16979182-16979201 | Msa0444140:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | + | chr3_3:16979650-16979669 | None:intergenic | 30.0% | |
| ATTTCCCAATGATACACAAC+AGG | - | chr3_3:16978971-16978990 | Msa0444140:intron | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | - | chr3_3:16979020-16979039 | Msa0444140:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | - | chr3_3:16979300-16979319 | Msa0444140:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | - | chr3_3:16979544-16979563 | Msa0444140:intron | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | + | chr3_3:16979644-16979663 | None:intergenic | 35.0% | |
| ATTTCCCAATGATACACAAC+AGG | - | chr3_3:16978971-16978990 | Msa0444140:intron | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | - | chr3_3:16979020-16979039 | Msa0444140:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | - | chr3_3:16979300-16979319 | Msa0444140:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | - | chr3_3:16979544-16979563 | Msa0444140:intron | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | + | chr3_3:16979644-16979663 | None:intergenic | 35.0% | |
| ! | TGTTGGCTACATTAAAGCGA+AGG | - | chr3_3:16978911-16978930 | Msa0444140:CDS | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | - | chr3_3:16978918-16978937 | Msa0444140:CDS | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | + | chr3_3:16979054-16979073 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | - | chr3_3:16979092-16979111 | Msa0444140:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | - | chr3_3:16979608-16979627 | Msa0444140:CDS | 40.0% |
| ! | TGTTGGCTACATTAAAGCGA+AGG | - | chr3_3:16978911-16978930 | Msa0444140:CDS | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | - | chr3_3:16978918-16978937 | Msa0444140:CDS | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | + | chr3_3:16979054-16979073 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | - | chr3_3:16979092-16979111 | Msa0444140:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | - | chr3_3:16979608-16979627 | Msa0444140:CDS | 40.0% |
| GGCTACATTAAAGCGAAGGA+AGG | - | chr3_3:16978915-16978934 | Msa0444140:CDS | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | + | chr3_3:16978954-16978973 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | + | chr3_3:16979167-16979186 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | + | chr3_3:16979313-16979332 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | + | chr3_3:16979322-16979341 | None:intergenic | 45.0% | |
| TCAGATGAAAGCAACGCACA+TGG | - | chr3_3:16979668-16979687 | Msa0444140:CDS | 45.0% | |
| GGCTACATTAAAGCGAAGGA+AGG | - | chr3_3:16978915-16978934 | Msa0444140:CDS | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | + | chr3_3:16978954-16978973 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | + | chr3_3:16979167-16979186 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | + | chr3_3:16979313-16979332 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | + | chr3_3:16979322-16979341 | None:intergenic | 45.0% | |
| TCAGATGAAAGCAACGCACA+TGG | - | chr3_3:16979668-16979687 | Msa0444140:CDS | 45.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | - | chr3_3:16978894-16978913 | Msa0444140:CDS | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | - | chr3_3:16979318-16979337 | Msa0444140:intron | 50.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | - | chr3_3:16978894-16978913 | Msa0444140:CDS | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | - | chr3_3:16979318-16979337 | Msa0444140:intron | 50.0% | |
| AAGGCAGCAGCTAACTCTGG+AGG | + | chr3_3:16978951-16978970 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | + | chr3_3:16979307-16979326 | None:intergenic | 55.0% |
| AAGGCAGCAGCTAACTCTGG+AGG | + | chr3_3:16978951-16978970 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | + | chr3_3:16979307-16979326 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 16978888 | 16979706 | 16978888 | ID=Msa0444140;Name=Msa0444140 |
| chr3_3 | mRNA | 16978888 | 16979706 | 16978888 | ID=Msa0444140-mRNA-1;Parent=Msa0444140;Name=Msa0444140-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|1|2|0|64 |
| chr3_3 | exon | 16979602 | 16979706 | 16979602 | ID=Msa0444140-mRNA-1:exon:3526;Parent=Msa0444140-mRNA-1 |
| chr3_3 | exon | 16978888 | 16978977 | 16978888 | ID=Msa0444140-mRNA-1:exon:3525;Parent=Msa0444140-mRNA-1 |
| chr3_3 | CDS | 16979602 | 16979706 | 16979602 | ID=Msa0444140-mRNA-1:cds;Parent=Msa0444140-mRNA-1 |
| chr3_3 | CDS | 16978888 | 16978977 | 16978888 | ID=Msa0444140-mRNA-1:cds;Parent=Msa0444140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
