Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1015450 | XP_039682656.1 | 94.495 | 109 | 6 | 0 | 4 | 112 | 56 | 164 | 1.04e-66 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1015450 | G7ZWU3 | 87.500 | 112 | 14 | 0 | 1 | 112 | 2 | 113 | 2.01e-64 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0739350 | Msa1015450 | 0.846190 | 2.537106e-59 | -8.615850e-47 |
| Msa0777190 | Msa1015450 | 0.878666 | 2.335349e-69 | -8.615850e-47 |
| Msa0444140 | Msa1015450 | 0.851894 | 6.592338e-61 | -8.615850e-47 |
| Msa1015450 | Msa1133620 | 0.801995 | 6.921110e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1015450 | MtrunA17_Chr5g0419101 | 92.157 | 102 | 8 | 0 | 8 | 109 | 1 | 102 | 1.21e-65 | 193 |
| Msa1015450 | MtrunA17_Chr2g0309021 | 92.453 | 106 | 8 | 0 | 4 | 109 | 421 | 526 | 4.87e-54 | 176 |
| Msa1015450 | MtrunA17_Chr8g0356411 | 93.443 | 61 | 4 | 0 | 52 | 112 | 1 | 61 | 3.06e-36 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACTTAGCGGTATGGAGTT+TGG | 0.349302 | 7_1:-27923298 | Msa1015450:CDS |
| TTGTGTATCATTGGGAAATA+AGG | 0.351637 | 7_1:+27923135 | None:intergenic |
| TTATACCTGTTGTGTATCAT+TGG | 0.404371 | 7_1:+27923126 | None:intergenic |
| GATGATCTAGATATGTCATT+AGG | 0.423580 | 7_1:+27922454 | None:intergenic |
| TGCAACTATGGAGGCTCAAA+TGG | 0.437214 | 7_1:-27923221 | Msa1015450:CDS |
| GCTCAAATGGCAGCACTTGT+TGG | 0.439947 | 7_1:-27923208 | Msa1015450:CDS |
| GGTCACCGTGTTCGAGGAAT+GGG | 0.468678 | 7_1:-27923364 | Msa1015450:CDS |
| ATTTCCCAATGATACACAAC+AGG | 0.483592 | 7_1:-27923131 | Msa1015450:intron |
| AATAAGGCAGCAGCTAACTC+TGG | 0.498255 | 7_1:+27923151 | None:intergenic |
| GGGTCACCGTGTTCGAGGAA+TGG | 0.501193 | 7_1:-27923365 | Msa1015450:CDS |
| GTATGCTTAAAAGCAATAGT+AGG | 0.508467 | 7_1:+27923328 | None:intergenic |
| CGTGTTCGAGGAATGGGAAT+GGG | 0.509239 | 7_1:-27923358 | Msa1015450:CDS |
| TTTATGGGTCACCGTGTTCG+AGG | 0.514731 | 7_1:-27923370 | Msa1015450:CDS |
| TTCAAGCGGCAGTTCTGCAT+TGG | 0.518225 | 7_1:-27923257 | Msa1015450:CDS |
| TATACCTGTTGTGTATCATT+GGG | 0.541600 | 7_1:+27923127 | None:intergenic |
| CTAGATATGTCATTAGGTGT+TGG | 0.547570 | 7_1:+27922460 | None:intergenic |
| CATACTACCACACGACTTAG+CGG | 0.549024 | 7_1:-27923310 | Msa1015450:CDS |
| CCGTGTTCGAGGAATGGGAA+TGG | 0.549061 | 7_1:-27923359 | Msa1015450:CDS |
| TCGAGGAATGGGAATGGGAG+TGG | 0.575002 | 7_1:-27923353 | Msa1015450:CDS |
| TACATTAAAGCGAAGGAAGG+TGG | 0.589028 | 7_1:-27923184 | Msa1015450:CDS |
| TACCACACGACTTAGCGGTA+TGG | 0.593195 | 7_1:-27923305 | Msa1015450:CDS |
| TGTTGGCTACATTAAAGCGA+AGG | 0.611559 | 7_1:-27923191 | Msa1015450:CDS |
| GGCTACATTAAAGCGAAGGA+AGG | 0.617737 | 7_1:-27923187 | Msa1015450:CDS |
| CTCCATACCGCTAAGTCGTG+TGG | 0.620607 | 7_1:+27923303 | None:intergenic |
| CCATTCCCATTCCTCGAACA+CGG | 0.623378 | 7_1:+27923359 | None:intergenic |
| TTTCGTGGTAGTACTTCAAG+CGG | 0.647511 | 7_1:-27923271 | Msa1015450:CDS |
| TCAGATGAAAGCAACGCACG+TGG | 0.667433 | 7_1:-27922433 | Msa1015450:CDS |
| AAGGCAGCAGCTAACTCTGG+AGG | 0.673734 | 7_1:+27923154 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATAAATGAAAGTTTTAA+GGG | - | chr7_1:27922917-27922936 | Msa1015450:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | - | chr7_1:27923037-27923056 | Msa1015450:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | - | chr7_1:27923162-27923181 | Msa1015450:CDS | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | - | chr7_1:27923278-27923297 | Msa1015450:CDS | 15.0% |
| !!! | AGAATAAATGAAAGTTTTAA+GGG | - | chr7_1:27922917-27922936 | Msa1015450:intron | 15.0% |
| !!! | TTTGATGATATTTTACAAAA+TGG | - | chr7_1:27923037-27923056 | Msa1015450:intron | 15.0% |
| !!! | CAATTATGAAAAATTGATTT+TGG | - | chr7_1:27923162-27923181 | Msa1015450:CDS | 15.0% |
| !!! | CAATTATAATATTTTTTTGC+AGG | - | chr7_1:27923278-27923297 | Msa1015450:CDS | 15.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | + | chr7_1:27922800-27922819 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | + | chr7_1:27922801-27922820 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | - | chr7_1:27922916-27922935 | Msa1015450:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | + | chr7_1:27922950-27922969 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | - | chr7_1:27923215-27923234 | Msa1015450:CDS | 20.0% |
| !!! | CTTCACTTATTTTATAACTT+GGG | + | chr7_1:27922800-27922819 | None:intergenic | 20.0% |
| !!! | TCTTCACTTATTTTATAACT+TGG | + | chr7_1:27922801-27922820 | None:intergenic | 20.0% |
| !!! | GAGAATAAATGAAAGTTTTA+AGG | - | chr7_1:27922916-27922935 | Msa1015450:intron | 20.0% |
| !! | TCATATTACATAACAACATT+TGG | + | chr7_1:27922950-27922969 | None:intergenic | 20.0% |
| !!! | TAATAACTTTTACTTTGCTT+AGG | - | chr7_1:27923215-27923234 | Msa1015450:CDS | 20.0% |
| ! | TATATATTTAGCAATGAGGA+AGG | + | chr7_1:27922743-27922762 | None:intergenic | 25.0% |
| ! | TCTCTATATATTTAGCAATG+AGG | + | chr7_1:27922747-27922766 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | - | chr7_1:27922923-27922942 | Msa1015450:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | - | chr7_1:27923053-27923072 | Msa1015450:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | - | chr7_1:27923054-27923073 | Msa1015450:intron | 25.0% |
| ! | TATATATTTAGCAATGAGGA+AGG | + | chr7_1:27922743-27922762 | None:intergenic | 25.0% |
| ! | TCTCTATATATTTAGCAATG+AGG | + | chr7_1:27922747-27922766 | None:intergenic | 25.0% |
| !!! | AATGAAAGTTTTAAGGGTTA+CGG | - | chr7_1:27922923-27922942 | Msa1015450:intron | 25.0% |
| ! | AAAATGGATCTTAATGCTAT+TGG | - | chr7_1:27923053-27923072 | Msa1015450:intron | 25.0% |
| ! | AAATGGATCTTAATGCTATT+GGG | - | chr7_1:27923054-27923073 | Msa1015450:intron | 25.0% |
| GTATGCTTAAAAGCAATAGT+AGG | + | chr7_1:27922459-27922478 | None:intergenic | 30.0% | |
| TTGTGTATCATTGGGAAATA+AGG | + | chr7_1:27922652-27922671 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | + | chr7_1:27922660-27922679 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | + | chr7_1:27922661-27922680 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | + | chr7_1:27922792-27922811 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | + | chr7_1:27922827-27922846 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | - | chr7_1:27922864-27922883 | Msa1015450:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | + | chr7_1:27923333-27923352 | None:intergenic | 30.0% | |
| GTATGCTTAAAAGCAATAGT+AGG | + | chr7_1:27922459-27922478 | None:intergenic | 30.0% | |
| TTGTGTATCATTGGGAAATA+AGG | + | chr7_1:27922652-27922671 | None:intergenic | 30.0% | |
| TATACCTGTTGTGTATCATT+GGG | + | chr7_1:27922660-27922679 | None:intergenic | 30.0% | |
| TTATACCTGTTGTGTATCAT+TGG | + | chr7_1:27922661-27922680 | None:intergenic | 30.0% | |
| ! | ATTTTATAACTTGGGCCTTT+TGG | + | chr7_1:27922792-27922811 | None:intergenic | 30.0% |
| ! | GATTAGTCATTATCAACTCT+TGG | + | chr7_1:27922827-27922846 | None:intergenic | 30.0% |
| ! | ATCAGTAAAACGCAATTTTG+TGG | - | chr7_1:27922864-27922883 | Msa1015450:intron | 30.0% |
| GATGATCTAGATATGTCATT+AGG | + | chr7_1:27923333-27923352 | None:intergenic | 30.0% | |
| GAACCAAAAACTTGCAACTA+TGG | - | chr7_1:27922551-27922570 | Msa1015450:intron | 35.0% | |
| ATTTCCCAATGATACACAAC+AGG | - | chr7_1:27922653-27922672 | Msa1015450:intron | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | - | chr7_1:27922702-27922721 | Msa1015450:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | - | chr7_1:27922982-27923001 | Msa1015450:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | - | chr7_1:27923227-27923246 | Msa1015450:CDS | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | + | chr7_1:27923327-27923346 | None:intergenic | 35.0% | |
| GAACCAAAAACTTGCAACTA+TGG | - | chr7_1:27922551-27922570 | Msa1015450:intron | 35.0% | |
| ATTTCCCAATGATACACAAC+AGG | - | chr7_1:27922653-27922672 | Msa1015450:intron | 35.0% | |
| ! | ATTTTGTGCATGTGTCTTTC+TGG | - | chr7_1:27922702-27922721 | Msa1015450:intron | 35.0% |
| TATACCACTACCACTATTAC+CGG | - | chr7_1:27922982-27923001 | Msa1015450:intron | 35.0% | |
| ! | CTTTGCTTAGGTTGTTGAAT+TGG | - | chr7_1:27923227-27923246 | Msa1015450:CDS | 35.0% |
| CTAGATATGTCATTAGGTGT+TGG | + | chr7_1:27923327-27923346 | None:intergenic | 35.0% | |
| TTTCGTGGTAGTACTTCAAG+CGG | - | chr7_1:27922513-27922532 | Msa1015450:intron | 40.0% | |
| ! | CCTCCATAGTTGCAAGTTTT+TGG | + | chr7_1:27922557-27922576 | None:intergenic | 40.0% |
| CCAAAAACTTGCAACTATGG+AGG | - | chr7_1:27922554-27922573 | Msa1015450:intron | 40.0% | |
| ! | TGTTGGCTACATTAAAGCGA+AGG | - | chr7_1:27922593-27922612 | Msa1015450:intron | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | - | chr7_1:27922600-27922619 | Msa1015450:intron | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | + | chr7_1:27922736-27922755 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | - | chr7_1:27922774-27922793 | Msa1015450:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | - | chr7_1:27923291-27923310 | Msa1015450:CDS | 40.0% |
| TTTCGTGGTAGTACTTCAAG+CGG | - | chr7_1:27922513-27922532 | Msa1015450:intron | 40.0% | |
| ! | CCTCCATAGTTGCAAGTTTT+TGG | + | chr7_1:27922557-27922576 | None:intergenic | 40.0% |
| CCAAAAACTTGCAACTATGG+AGG | - | chr7_1:27922554-27922573 | Msa1015450:intron | 40.0% | |
| ! | TGTTGGCTACATTAAAGCGA+AGG | - | chr7_1:27922593-27922612 | Msa1015450:intron | 40.0% |
| TACATTAAAGCGAAGGAAGG+TGG | - | chr7_1:27922600-27922619 | Msa1015450:intron | 40.0% | |
| TTAGCAATGAGGAAGGTTGT+AGG | + | chr7_1:27922736-27922755 | None:intergenic | 40.0% | |
| CTAGTTGCGTAGAATCCAAA+AGG | - | chr7_1:27922774-27922793 | Msa1015450:intron | 40.0% | |
| ! | TTTTTGCAGGCATCAAACGT+AGG | - | chr7_1:27923291-27923310 | Msa1015450:CDS | 40.0% |
| CATACTACCACACGACTTAG+CGG | - | chr7_1:27922474-27922493 | Msa1015450:CDS | 45.0% | |
| !!! | ATGGAGTTTGGCTCTTTTCG+TGG | - | chr7_1:27922498-27922517 | Msa1015450:intron | 45.0% |
| TGCAACTATGGAGGCTCAAA+TGG | - | chr7_1:27922563-27922582 | Msa1015450:intron | 45.0% | |
| GGCTACATTAAAGCGAAGGA+AGG | - | chr7_1:27922597-27922616 | Msa1015450:intron | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | + | chr7_1:27922636-27922655 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | + | chr7_1:27922849-27922868 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | + | chr7_1:27922995-27923014 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | + | chr7_1:27923004-27923023 | None:intergenic | 45.0% | |
| CATACTACCACACGACTTAG+CGG | - | chr7_1:27922474-27922493 | Msa1015450:CDS | 45.0% | |
| !!! | ATGGAGTTTGGCTCTTTTCG+TGG | - | chr7_1:27922498-27922517 | Msa1015450:intron | 45.0% |
| TGCAACTATGGAGGCTCAAA+TGG | - | chr7_1:27922563-27922582 | Msa1015450:intron | 45.0% | |
| GGCTACATTAAAGCGAAGGA+AGG | - | chr7_1:27922597-27922616 | Msa1015450:intron | 45.0% | |
| AATAAGGCAGCAGCTAACTC+TGG | + | chr7_1:27922636-27922655 | None:intergenic | 45.0% | |
| CTGATCATGTCTGAACGTGA+TGG | + | chr7_1:27922849-27922868 | None:intergenic | 45.0% | |
| !! | TAATGTAGCGCCGGTAATAG+TGG | + | chr7_1:27922995-27923014 | None:intergenic | 45.0% |
| TCCAAAGCTTAATGTAGCGC+CGG | + | chr7_1:27923004-27923023 | None:intergenic | 45.0% | |
| CCATTCCCATTCCTCGAACA+CGG | + | chr7_1:27922428-27922447 | None:intergenic | 50.0% | |
| CGTGTTCGAGGAATGGGAAT+GGG | - | chr7_1:27922426-27922445 | Msa1015450:CDS | 50.0% | |
| TACCACACGACTTAGCGGTA+TGG | - | chr7_1:27922479-27922498 | Msa1015450:CDS | 50.0% | |
| CGACTTAGCGGTATGGAGTT+TGG | - | chr7_1:27922486-27922505 | Msa1015450:CDS | 50.0% | |
| TTCAAGCGGCAGTTCTGCAT+TGG | - | chr7_1:27922527-27922546 | Msa1015450:intron | 50.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | - | chr7_1:27922576-27922595 | Msa1015450:intron | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | - | chr7_1:27923000-27923019 | Msa1015450:intron | 50.0% | |
| TCAGATGAAAGCAACGCACG+TGG | - | chr7_1:27923351-27923370 | Msa1015450:CDS | 50.0% | |
| CCATTCCCATTCCTCGAACA+CGG | + | chr7_1:27922428-27922447 | None:intergenic | 50.0% | |
| CGTGTTCGAGGAATGGGAAT+GGG | - | chr7_1:27922426-27922445 | Msa1015450:CDS | 50.0% | |
| TACCACACGACTTAGCGGTA+TGG | - | chr7_1:27922479-27922498 | Msa1015450:CDS | 50.0% | |
| CGACTTAGCGGTATGGAGTT+TGG | - | chr7_1:27922486-27922505 | Msa1015450:CDS | 50.0% | |
| TTCAAGCGGCAGTTCTGCAT+TGG | - | chr7_1:27922527-27922546 | Msa1015450:intron | 50.0% | |
| GCTCAAATGGCAGCACTTGT+TGG | - | chr7_1:27922576-27922595 | Msa1015450:intron | 50.0% | |
| ACCGGCGCTACATTAAGCTT+TGG | - | chr7_1:27923000-27923019 | Msa1015450:intron | 50.0% | |
| TCAGATGAAAGCAACGCACG+TGG | - | chr7_1:27923351-27923370 | Msa1015450:CDS | 50.0% | |
| GGTCACCGTGTTCGAGGAAT+GGG | - | chr7_1:27922420-27922439 | Msa1015450:CDS | 55.0% | |
| CCGTGTTCGAGGAATGGGAA+TGG | - | chr7_1:27922425-27922444 | Msa1015450:CDS | 55.0% | |
| TCGAGGAATGGGAATGGGAG+TGG | - | chr7_1:27922431-27922450 | Msa1015450:CDS | 55.0% | |
| CTCCATACCGCTAAGTCGTG+TGG | + | chr7_1:27922484-27922503 | None:intergenic | 55.0% | |
| AAGGCAGCAGCTAACTCTGG+AGG | + | chr7_1:27922633-27922652 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | + | chr7_1:27922989-27923008 | None:intergenic | 55.0% |
| GGTCACCGTGTTCGAGGAAT+GGG | - | chr7_1:27922420-27922439 | Msa1015450:CDS | 55.0% | |
| CCGTGTTCGAGGAATGGGAA+TGG | - | chr7_1:27922425-27922444 | Msa1015450:CDS | 55.0% | |
| TCGAGGAATGGGAATGGGAG+TGG | - | chr7_1:27922431-27922450 | Msa1015450:CDS | 55.0% | |
| CTCCATACCGCTAAGTCGTG+TGG | + | chr7_1:27922484-27922503 | None:intergenic | 55.0% | |
| AAGGCAGCAGCTAACTCTGG+AGG | + | chr7_1:27922633-27922652 | None:intergenic | 55.0% | |
| !! | AGCGCCGGTAATAGTGGTAG+TGG | + | chr7_1:27922989-27923008 | None:intergenic | 55.0% |
| GGGTCACCGTGTTCGAGGAA+TGG | - | chr7_1:27922419-27922438 | Msa1015450:CDS | 60.0% | |
| GGGTCACCGTGTTCGAGGAA+TGG | - | chr7_1:27922419-27922438 | Msa1015450:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 27922417 | 27923389 | 27922417 | ID=Msa1015450;Name=Msa1015450 |
| chr7_1 | mRNA | 27922417 | 27923389 | 27922417 | ID=Msa1015450-mRNA-1;Parent=Msa1015450;Name=Msa1015450-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|1|2|0|115 |
| chr7_1 | exon | 27923132 | 27923389 | 27923132 | ID=Msa1015450-mRNA-1:exon:5959;Parent=Msa1015450-mRNA-1 |
| chr7_1 | exon | 27922417 | 27922506 | 27922417 | ID=Msa1015450-mRNA-1:exon:5958;Parent=Msa1015450-mRNA-1 |
| chr7_1 | CDS | 27923132 | 27923389 | 27923132 | ID=Msa1015450-mRNA-1:cds;Parent=Msa1015450-mRNA-1 |
| chr7_1 | CDS | 27922417 | 27922506 | 27922417 | ID=Msa1015450-mRNA-1:cds;Parent=Msa1015450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
