AlfalfaGEDB | Msa0444320

M. sativa cultivar zhongmu-4 / Msa0444320

KEGG: -
GO: -
PFAM: PF00954.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444320	XP_013459009.2	81.379	580	78	4	96	668	1081	1637	0.0	965
Msa0444320	XP_013459009.2	86.014	572	51	3	96	660	242	791	0.0	984

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444320	sp\|O81832\|Y4729_ARATH	47.772	561	226	8	108	660	255	756	4.67e-172	513

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444320	G7IYX6	86.014	572	51	3	96	660	242	791	0.0	983

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0088550	Msa0444320	0.800050	1.728804e-48	-8.615850e-47
Msa0444320	Msa0448550	-0.809590	1.755231e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0444320	MtrunA17_Chr3g0084421	86.014	572	51	3	96	660	204	753	982
Msa0444320	MtrunA17_Chr3g0084411	81.379	580	78	4	96	668	242	798	967
Msa0444320	MtrunA17_Chr3g0084441	77.138	573	101	5	96	660	248	798	900
Msa0444320	MtrunA17_Chr3g0084451	75.862	580	104	6	96	661	242	799	890
Msa0444320	MtrunA17_Chr3g0084691	74.329	596	100	6	96	661	243	815	872
Msa0444320	MtrunA17_Chr3g0084491	74.697	577	108	7	96	664	256	802	864
Msa0444320	MtrunA17_Chr3g0084431	75.000	572	104	4	96	661	248	786	860
Msa0444320	MtrunA17_Chr3g0084681	72.518	564	86	5	109	664	258	760	816
Msa0444320	MtrunA17_Chr2g0318811	52.196	592	242	9	82	656	221	788	592
Msa0444320	MtrunA17_Chr8g0361251	51.973	583	240	8	96	661	239	798	571

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444320	AT4G27290.2	48.873	577	242	10	108	660	255	802	5.12e-179	530
Msa0444320	AT4G27290.1	47.772	561	226	8	108	660	255	756	4.74e-173	513
Msa0444320	AT4G27300.1	49.204	565	243	10	109	660	258	791	6.34e-167	499
Msa0444320	AT1G65790.1	43.428	601	271	8	108	667	254	826	1.91e-158	478
Msa0444320	AT4G21380.1	43.939	594	247	9	109	655	261	815	2.11e-158	478
Msa0444320	AT1G65800.1	44.863	584	265	10	108	659	259	817	1.07e-155	471
Msa0444320	AT1G11340.1	43.174	586	270	13	108	659	248	804	2.81e-144	441
Msa0444320	AT1G11410.2	42.929	594	273	13	108	663	136	701	1.21e-141	431
Msa0444320	AT1G11410.3	42.929	594	273	13	108	663	227	792	3.24e-140	430
Msa0444320	AT1G11410.4	42.929	594	273	13	108	663	258	823	3.33e-140	431

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Find 137 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACAGATGACATATT+AGG	0.408318	3_3:+17244646	None:intergenic
AAAAGGCATAAGCATCACAC+TGG	0.680768	3_3:+17247365	None:intergenic
AAACCAAAATCGGATATCTT+TGG	0.277143	3_3:+17245680	None:intergenic
AAACGTTGTGATAATCCATT+TGG	0.328464	3_3:+17247442	None:intergenic
AAACTATGACTAGGGCGTAC+TGG	0.518726	3_3:-17246155	Msa0444320:intron
AAATTATTGACGGTATTGCT+CGG	0.505258	3_3:-17245793	Msa0444320:CDS
AACATCGGGACAAGGAACTG+AGG	0.697949	3_3:-17246095	Msa0444320:CDS
AACCAAAATCGGATATCTTT+GGG	0.357407	3_3:+17245681	None:intergenic
AACCACGATGCTGACGTATC+TGG	0.421235	3_3:+17247163	None:intergenic
AACCCGGGTTCACTTGGTTT+TGG	0.236113	3_3:+17244598	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAGAAGAAAGTGGAG+TGG	-	chr3_3:17244313-17244332	Msa0444320:three_prime_UTR	30.0%
ATAAAGACAACATGGAAAAG+AGG	-	chr3_3:17245072-17245091	Msa0444320:CDS	30.0%
TAAAGACAACATGGAAAAGA+GGG	-	chr3_3:17245073-17245092	Msa0444320:CDS	30.0%
TCACTCAATTCATTCAAGAT+TGG	+	chr3_3:17245221-17245240	None:intergenic	30.0%
CAGTTCAATTATTACTAGAG+TGG	-	chr3_3:17245598-17245617	Msa0444320:intron	30.0%
AACGTTGTGATAATCCATTT+GGG	+	chr3_3:17245631-17245650	None:intergenic	30.0%
AAACGTTGTGATAATCCATT+TGG	+	chr3_3:17245632-17245651	None:intergenic	30.0%
GATTATCACAACGTTTACAA+TGG	-	chr3_3:17245636-17245655	Msa0444320:CDS	30.0%
GTTAGAATTGATACCACAAA+AGG	+	chr3_3:17245726-17245745	None:intergenic	30.0%
CTTTCCAATATGTGAATGTT+TGG	-	chr3_3:17245760-17245779	Msa0444320:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	17244283	17248810	17244283	ID=Msa0444320;Name=Msa0444320
chr3_3	mRNA	17244283	17248810	17244283	ID=Msa0444320-mRNA-1;Parent=Msa0444320;Name=Msa0444320-mRNA-1;_AED=0.35;_eAED=0.36;_QI=154\|0.5\|0.44\|1\|0.87\|0.77\|9\|266\|670
chr3_3	exon	17244283	17244803	17244283	ID=Msa0444320-mRNA-1:exon:3670;Parent=Msa0444320-mRNA-1
chr3_3	exon	17245035	17245265	17245035	ID=Msa0444320-mRNA-1:exon:3669;Parent=Msa0444320-mRNA-1
chr3_3	exon	17245634	17245892	17245634	ID=Msa0444320-mRNA-1:exon:3668;Parent=Msa0444320-mRNA-1
chr3_3	exon	17246007	17246164	17246007	ID=Msa0444320-mRNA-1:exon:3667;Parent=Msa0444320-mRNA-1
chr3_3	exon	17246255	17246406	17246255	ID=Msa0444320-mRNA-1:exon:3666;Parent=Msa0444320-mRNA-1
chr3_3	exon	17246567	17246683	17246567	ID=Msa0444320-mRNA-1:exon:3665;Parent=Msa0444320-mRNA-1
chr3_3	exon	17246972	17247492	17246972	ID=Msa0444320-mRNA-1:exon:3664;Parent=Msa0444320-mRNA-1
chr3_3	exon	17247706	17248030	17247706	ID=Msa0444320-mRNA-1:exon:3663;Parent=Msa0444320-mRNA-1
chr3_3	exon	17248662	17248810	17248662	ID=Msa0444320-mRNA-1:exon:3662;Parent=Msa0444320-mRNA-1
chr3_3	five_prime_UTR	17248662	17248810	17248662	ID=Msa0444320-mRNA-1:five_prime_utr;Parent=Msa0444320-mRNA-1
chr3_3	five_prime_UTR	17248026	17248030	17248026	ID=Msa0444320-mRNA-1:five_prime_utr;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17247706	17248025	17247706	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17246972	17247492	17246972	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17246567	17246683	17246567	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17246255	17246406	17246255	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17246007	17246164	17246007	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17245634	17245892	17245634	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17245035	17245265	17245035	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	CDS	17244549	17244803	17244549	ID=Msa0444320-mRNA-1:cds;Parent=Msa0444320-mRNA-1
chr3_3	three_prime_UTR	17244283	17244548	17244283	ID=Msa0444320-mRNA-1:three_prime_utr;Parent=Msa0444320-mRNA-1

Gene Sequence

>Msa0444320

ATGAATAAAGACAACATGGAAAAGAGGGAATTTTCGGATTTGCTTTCAACGCTGAAAGTTTTGGCTGATGAAGTTGTTTCGTTTGCTAAGAAATCTGAAATTGAGGTAGATGCCTTTTCTGAGTTTTCCATGTTGGTTGAGAAATTACCACCAATCTTGAATGAATTGAGTGATAACAGCGTTGTATTGGACAAGCCATCAATTCGAAAATCGTTGGAATCTCTTGAGAATGAGCTACGGCGTGCTAAAGCTTTGACGAAAAGCTCAAATTTGAGACACCCTATTAAGCAAATTGAGGACATGACGCATGATATCGGCCGTTCAATTATTACTAGAGTGGTGCTAGACCCAAATGGATTATCACAACGTTTACAATGGACAGATAGGACACAGAATTGGGCAGCTATAGCTAATCGTCCTGCAGACCAGTGTGATGCTTATGCCTTTTGTGGTATCAATTCTAACTGCAATAGTAATGACTTTCCAATATGTGAATGTTTGGAAGGTTTCATGCCAAAATTTCAACCAAAATGGGAGTCATCAAATTGGTCTGGTGGGTGTGTAAGGAAAACACATTTAACTTGTCTCCATGGAGATGGATTTTTACCATACACAAACATGAAGTTGCCAGATACGTCAGCATCGTGGTTTGACAAAACCTTGAGCCTCGAGGAATGTAAGACAATGTGTTTGAAAAACTGTTCTTGCACTGCATATGCAACTTTAGATATCAGAGATGGTGGTAGTGGCTGCATACTCTGGTTTAACAACATTGTGGACATGAGAATACATCAAGACCAAGGACAAGACATTTACATATGGATGGCATCTTCAGAACTTGATCATAAAAAGAATAAGCAGAAGTTGAAGCTTGCTGGGACTCTTGCAGGAGTTATTGCATTCATTATAGGACTAATAGTTCTTGTATTAGTCACATCAGCGTATAAAAAGAAGCTTGGGTATATAAAAAAGTTATTTCTCTGGAAGCACAAGGAGAAGGAAGATGGTGACTTGGCTACAATATTTGATTTTTCAACCATCACTAATGCAACAAATAACTTCTCTGTCAGAAACAAGTTGAGAGAAGGTGGTCTTGGACCAGTGTACAAGGGCGTACTGGTAGACGGCCAAGAGATTGCTGTTAAGAGGCTATCAAAAACATCGGGACAAGGAACTGAGGAGTTCAAAAATGAAGTAAAGTTGATGGCAACACTTCAACACCGTAATCTTGTAAAACTTCTTGGTTGTTCTATTCAACACAATGAAAATTCTCATGCTAACTTAAGAATGTGTCATTTGTTAGATACTGCACGAAGTAAATTACTAGATTGGACTAAGCGTTTGGAAATTATTGACGGTATTGCTCGGGGGCTGTTGTATCTCCATCAGGATTCTACATTAAGAATCATACATAGAGATCTCAAGACAAGCAATATTCTTCTTGATATTGATATGATCCCAAAGATATCCGATTTTGGTTTAGCCAGATCATTTATGGGAGACCAAGCTGAGGCTAATACAAATAGAGTACTGAAAACCTTCATTTCTGCTATGTCACAACCCCTTTTAATATTATATTTTTCCAACTTCAATGTTCTTTCTTGCAGTGGATATATGCCTCCAGAATATGCGGTGCATTGGTCTTTCTCAATAAAATCTGATGTTTTTAGTTTTGGAGTCGTTGTACTTGAGATCATTAGCGGTAGGAAGAATCGTGGATTTTGTGACCCTCTGCATCATCTAAACCTTCTTGGTCATGCATGGAGACTGTGGATTGAAGGCAGGCCAGAGGAGTTAATAGCTGACATATTATATGATGAGGCCATATGTTCAGAAATAATAAGATTTATTCATGTTGGTCTATTGTGTGTGCAACAGAAACCAGCAAATAGACCTAATATGTCATCTGTGGTTTTTATGTTGAAGGGTGAAAAGGTACTCCCAAAACCAAGTGAACCCGGGTTCTACGCAGGAAGAGATAATAATAGTAATCAGCACAGGATCTTCATCTAA

Protein sequence

>Msa0444320

MNKDNMEKREFSDLLSTLKVLADEVVSFAKKSEIEVDAFSEFSMLVEKLPPILNELSDNSVVLDKPSIRKSLESLENELRRAKALTKSSNLRHPIKQIEDMTHDIGRSIITRVVLDPNGLSQRLQWTDRTQNWAAIANRPADQCDAYAFCGINSNCNSNDFPICECLEGFMPKFQPKWESSNWSGGCVRKTHLTCLHGDGFLPYTNMKLPDTSASWFDKTLSLEECKTMCLKNCSCTAYATLDIRDGGSGCILWFNNIVDMRIHQDQGQDIYIWMASSELDHKKNKQKLKLAGTLAGVIAFIIGLIVLVLVTSAYKKKLGYIKKLFLWKHKEKEDGDLATIFDFSTITNATNNFSVRNKLREGGLGPVYKGVLVDGQEIAVKRLSKTSGQGTEEFKNEVKLMATLQHRNLVKLLGCSIQHNENSHANLRMCHLLDTARSKLLDWTKRLEIIDGIARGLLYLHQDSTLRIIHRDLKTSNILLDIDMIPKISDFGLARSFMGDQAEANTNRVLKTFISAMSQPLLILYFSNFNVLSCSGYMPPEYAVHWSFSIKSDVFSFGVVVLEIISGRKNRGFCDPLHHLNLLGHAWRLWIEGRPEELIADILYDEAICSEIIRFIHVGLLCVQQKPANRPNMSSVVFMLKGEKVLPKPSEPGFYAGRDNNSNQHRIFI*