Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0445210 | Msa1314280 | 0.800668 | 1.293505e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACAGTCTACGTGATAAAT+TGG | 0.265078 | 3_3:+19161494 | Msa0445210:CDS |
| CACATAGCAGTGAACATGTT+TGG | 0.368728 | 3_3:-19161532 | None:intergenic |
| ATGGGAGCTTCCATGGTGTT+TGG | 0.430762 | 3_3:+19161470 | Msa0445210:CDS |
| ATCACTGTCCCCATGGTGAA+TGG | 0.442675 | 3_3:+19161451 | Msa0445210:CDS |
| GTCTACGTGATAAATTGGTT+TGG | 0.445163 | 3_3:+19161499 | Msa0445210:CDS |
| GATAAATTGGTTTGGAACTT+CGG | 0.454366 | 3_3:+19161507 | Msa0445210:CDS |
| TCTTAGTCTTATAGTTAAGC+TGG | 0.479859 | 3_3:-19161580 | None:intergenic |
| AACAGTTCTTCATTGTATCA+AGG | 0.483535 | 3_3:+19161556 | Msa0445210:CDS |
| TCACTGTCCCCATGGTGAAT+GGG | 0.500391 | 3_3:+19161452 | Msa0445210:CDS |
| ATGGTGAATGGGAGCTTCCA+TGG | 0.506530 | 3_3:+19161463 | Msa0445210:CDS |
| TGGAAGCTCCCATTCACCAT+GGG | 0.557821 | 3_3:-19161460 | None:intergenic |
| ATGGAAGCTCCCATTCACCA+TGG | 0.613360 | 3_3:-19161461 | None:intergenic |
| AGACTGTGTTCCAAACACCA+TGG | 0.705889 | 3_3:-19161480 | None:intergenic |
| GGAAGCTCCCATTCACCATG+GGG | 0.707799 | 3_3:-19161459 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAAATTGGTTTGGAACTT+CGG | + | chr3_3:19161507-19161526 | Msa0445210:CDS | 30.0% |
| AACAGTTCTTCATTGTATCA+AGG | + | chr3_3:19161556-19161575 | Msa0445210:CDS | 30.0% | |
| ACACAGTCTACGTGATAAAT+TGG | + | chr3_3:19161494-19161513 | Msa0445210:CDS | 35.0% | |
| GTCTACGTGATAAATTGGTT+TGG | + | chr3_3:19161499-19161518 | Msa0445210:CDS | 35.0% | |
| ! | GACAGTGATTTTTCTCGAGT+TGG | - | chr3_3:19161440-19161459 | None:intergenic | 40.0% |
| CACATAGCAGTGAACATGTT+TGG | - | chr3_3:19161535-19161554 | None:intergenic | 40.0% | |
| GAGAAAAATCACTGTCCCCA+TGG | + | chr3_3:19161444-19161463 | Msa0445210:CDS | 45.0% | |
| AGACTGTGTTCCAAACACCA+TGG | - | chr3_3:19161483-19161502 | None:intergenic | 45.0% | |
| ATCACTGTCCCCATGGTGAA+TGG | + | chr3_3:19161451-19161470 | Msa0445210:CDS | 50.0% | |
| TCACTGTCCCCATGGTGAAT+GGG | + | chr3_3:19161452-19161471 | Msa0445210:CDS | 50.0% | |
| TGGAAGCTCCCATTCACCAT+GGG | - | chr3_3:19161463-19161482 | None:intergenic | 50.0% | |
| ATGGAAGCTCCCATTCACCA+TGG | - | chr3_3:19161464-19161483 | None:intergenic | 50.0% | |
| ATGGTGAATGGGAGCTTCCA+TGG | + | chr3_3:19161463-19161482 | Msa0445210:CDS | 50.0% | |
| ATGGGAGCTTCCATGGTGTT+TGG | + | chr3_3:19161470-19161489 | Msa0445210:CDS | 50.0% | |
| GGAAGCTCCCATTCACCATG+GGG | - | chr3_3:19161462-19161481 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 19161430 | 19161588 | 19161430 | ID=Msa0445210;Name=Msa0445210 |
| chr3_3 | mRNA | 19161430 | 19161588 | 19161430 | ID=Msa0445210-mRNA-1;Parent=Msa0445210;Name=Msa0445210-mRNA-1;_AED=0.49;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|52 |
| chr3_3 | exon | 19161430 | 19161588 | 19161430 | ID=Msa0445210-mRNA-1:exon:4064;Parent=Msa0445210-mRNA-1 |
| chr3_3 | CDS | 19161430 | 19161588 | 19161430 | ID=Msa0445210-mRNA-1:cds;Parent=Msa0445210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
