AlfalfaGEDB | Msa1314280

M. sativa cultivar zhongmu-4 / Msa1314280

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314280	A0A396GDW0	54.286	70	14	2	6	75	1	52	4.74e-11	66.6

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0102070	Msa1314280	0.806856	6.712621e-50	-8.615850e-47
Msa0142220	Msa1314280	0.801805	7.572977e-49	-8.615850e-47
Msa0145750	Msa1314280	0.827789	1.288315e-54	-8.615850e-47
Msa0157810	Msa1314280	0.813028	3.148981e-51	-8.615850e-47
Msa0227480	Msa1314280	0.815584	8.579642e-52	-8.615850e-47
Msa0267000	Msa1314280	0.821043	4.979781e-53	-8.615850e-47
Msa0267010	Msa1314280	0.816190	6.284177e-52	-8.615850e-47
Msa0360510	Msa1314280	0.831511	1.602003e-55	-8.615850e-47
Msa0363300	Msa1314280	0.804566	2.030992e-49	-8.615850e-47
Msa0365500	Msa1314280	0.828189	1.032529e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314280	MtrunA17_Chr8g0343301	54.286	70	14	2	6	75	1	52	9.12e-15	66.6
Msa1314280	MtrunA17_Chr8g0343261	44.928	69	20	2	6	74	1	51	3.15e-11	57.4
Msa1314280	MtrunA17_Chr8g0343361	52.941	51	23	1	24	74	2	51	4.64e-11	57.0
Msa1314280	MtrunA17_Chr8g0343221	44.595	74	23	2	6	79	1	56	8.33e-11	56.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 24 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAAGGTTGGAGATCAA+AGG	0.540531	8_4:+13081366	Msa1314280:CDS
AACAATCAAGTTGATATTTA+TGG	0.139857	8_4:+13081259	Msa1314280:CDS
AACTCCAACCAATTGCAACG+TGG	0.713283	8_4:+13081294	Msa1314280:CDS
AAGAGGAAGCAAAGAAAGGT+TGG	0.488307	8_4:+13081356	Msa1314280:CDS
AATCAAGTTGATATTTATGG+TGG	0.565785	8_4:+13081262	Msa1314280:CDS
ACCAAAGAGGAAGCAAAGAA+AGG	0.547786	8_4:+13081352	Msa1314280:CDS
ACCTTTCTTTGCTTCCTCTT+TGG	0.208827	8_4:-13081353	None:intergenic
AGTGGAAACCCCAACCAAAG+AGG	0.641455	8_4:+13081339	Msa1314280:CDS
ATGAAGTCAATATTTATGAT+AGG	0.361327	8_4:+13080918	None:intergenic
CACTTGATTGTTCATTGTCT+GGG	0.318928	8_4:-13081320	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATAAAAACAAGGATCGA+TGG	+	chr8_4:13081416-13081435	Msa1314280:CDS	30.0%
ATAAAAATATGCAAGGTCCA+GGG	-	chr8_4:13081520-13081539	None:intergenic	30.0%
TGGTACCAATAATAACAGAT+TGG	+	chr8_4:13082042-13082061	Msa1314280:intron	30.0%
CTGAACCAATCTGTTATTAT+TGG	-	chr8_4:13082050-13082069	None:intergenic	30.0%
AGCTTGAAATGCAAATAGTT+CGG	+	chr8_4:13082108-13082127	Msa1314280:intron	30.0%
ATGAGTTACATCAATTGTTG+TGG	-	chr8_4:13082612-13082631	None:intergenic	30.0%
TTCATGGAATAACATGTACA+TGG	+	chr8_4:13083215-13083234	Msa1314280:intron	30.0%
TCATGGAATAACATGTACAT+GGG	+	chr8_4:13083216-13083235	Msa1314280:intron	30.0%
TTCATGACAACATCAAATCA+AGG	-	chr8_4:13083257-13083276	None:intergenic	30.0%
TTGATGTTGTCATGAATTTG+CGG	+	chr8_4:13083261-13083280	Msa1314280:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	13080933	13083841	13080933	ID=Msa1314280;Name=Msa1314280
chr8_4	mRNA	13080933	13083841	13080933	ID=Msa1314280-mRNA-1;Parent=Msa1314280;Name=Msa1314280-mRNA-1;_AED=0.53;_eAED=0.54;_QI=0\|0\|0\|0.33\|0\|0\|3\|0\|94
chr8_4	exon	13080933	13080939	13080933	ID=Msa1314280-mRNA-1:exon:4929;Parent=Msa1314280-mRNA-1
chr8_4	exon	13081248	13081463	13081248	ID=Msa1314280-mRNA-1:exon:4930;Parent=Msa1314280-mRNA-1
chr8_4	exon	13083780	13083841	13083780	ID=Msa1314280-mRNA-1:exon:4931;Parent=Msa1314280-mRNA-1
chr8_4	CDS	13080933	13080939	13080933	ID=Msa1314280-mRNA-1:cds;Parent=Msa1314280-mRNA-1
chr8_4	CDS	13081248	13081463	13081248	ID=Msa1314280-mRNA-1:cds;Parent=Msa1314280-mRNA-1
chr8_4	CDS	13083780	13083841	13083780	ID=Msa1314280-mRNA-1:cds;Parent=Msa1314280-mRNA-1

Gene Sequence	>Msa1314280 ATGATAGGTAAGACAATGAACAATCAAGTTGATATTTATGGTGGTTCAACAGAAACTCCAACCAATTGCAACGTGGAAACCCAGACAATGAACAATCAAGTGGAAACCCCAACCAAAGAGGAAGCAAAGAAAGGTTGGAGATCAAAGGTTTTAGAACAAGCAGTGGATTGGTTGAGATATAAAAACAAGGATCGATGGTTGGATAATATGAGAGGGACCATGGAGAAAGAGGAGATTACTTGTGATGCAAGTAAGCTCACAAATGAGGAAGAAGAAGTTACTTGA
Protein sequence	>Msa1314280 MIGKTMNNQVDIYGGSTETPTNCNVETQTMNNQVETPTKEEAKKGWRSKVLEQAVDWLRYKNKDRWLDNMRGTMEKEEITCDASKLTNEEEEVT*