AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0447120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0447120 MtrunA17_Chr3g0088301 98.715 389 5 0 1 389 1 389 0.0 788
Msa0447120 MtrunA17_Chr8g0352031 81.538 390 46 4 1 389 1 365 0.0 607
Msa0447120 MtrunA17_Chr4g0040341 74.872 390 76 3 1 388 1 370 0.0 546
Msa0447120 MtrunA17_Chr2g0328891 74.444 360 83 4 35 389 7 362 5.15e-178 499
Msa0447120 MtrunA17_Chr8g0393031 59.649 285 115 0 92 376 17 301 2.61e-128 371
Msa0447120 MtrunA17_Chr3g0135801 39.189 296 169 6 87 376 28 318 1.99e-67 216
Msa0447120 MtrunA17_Chr3g0135861 31.715 309 177 9 89 376 31 326 9.61e-36 134
Msa0447120 MtrunA17_Chr6g0488081 28.763 299 192 6 89 376 15 303 6.75e-33 125
Msa0447120 MtrunA17_Chr4g0006851 30.029 343 207 10 40 379 46 358 1.14e-28 115
Msa0447120 MtrunA17_Chr5g0396801 29.022 317 172 10 81 363 24 321 2.18e-28 114
Msa0447120 MtrunA17_Chr8g0347451 29.195 298 174 9 92 363 50 336 1.51e-27 111
Msa0447120 MtrunA17_Chr8g0347451 26.108 203 131 5 96 285 157 353 6.43e-11 63.2
Msa0447120 MtrunA17_Chr7g0240341 28.904 301 195 8 90 379 22 314 4.41e-27 109
Msa0447120 MtrunA17_Chr1g0149111 30.314 287 162 11 93 368 139 398 1.41e-26 110
Msa0447120 MtrunA17_Chr1g0149111 27.143 210 132 6 81 286 219 411 3.43e-14 73.6
Msa0447120 MtrunA17_Chr3g0137941 28.881 277 173 8 96 368 125 381 5.32e-26 108
Msa0447120 MtrunA17_Chr3g0137941 28.095 210 130 6 81 286 202 394 1.22e-15 77.8
Msa0447120 MtrunA17_Chr8g0385571 29.617 287 168 9 93 368 227 490 1.33e-25 108
Msa0447120 MtrunA17_Chr7g0252401 30.345 290 159 8 93 368 207 467 3.14e-25 107
Msa0447120 MtrunA17_Chr7g0242311 29.682 283 168 8 104 379 98 356 4.98e-25 105
Msa0447120 MtrunA17_Chr4g0067231 26.444 329 195 8 83 375 4 321 1.18e-24 103
Msa0447120 MtrunA17_Chr4g0063561 27.465 284 174 7 93 368 123 382 5.71e-24 102
Msa0447120 MtrunA17_Chr4g0063561 27.751 209 127 6 83 286 206 395 2.10e-14 74.3
Msa0447120 MtrunA17_Chr5g0436041 26.144 306 209 7 83 379 24 321 7.73e-24 100
Msa0447120 MtrunA17_Chr3g0131501 28.383 303 180 11 77 368 192 468 2.24e-23 101
Msa0447120 MtrunA17_Chr2g0296061 27.673 318 185 9 90 373 12 318 5.37e-23 98.6
Msa0447120 MtrunA17_Chr3g0115801 27.946 297 166 12 96 368 119 391 1.05e-17 84.3
Msa0447120 MtrunA17_Chr4g0000261 24.000 300 186 7 90 368 63 341 2.05e-17 82.8
Msa0447120 MtrunA17_Chr1g0196921 22.180 266 195 4 93 354 14 271 4.89e-17 80.9
Msa0447120 MtrunA17_Chr1g0196921 28.571 168 112 4 94 260 112 272 4.37e-13 69.3
Msa0447120 MtrunA17_Chr2g0331771 25.874 286 195 6 92 373 155 427 2.74e-16 80.1
Msa0447120 MtrunA17_Chr3g0133111 26.174 298 179 10 93 361 6 291 3.59e-16 78.6
Msa0447120 MtrunA17_Chr4g0004571 26.749 243 164 5 138 373 51 286 4.98e-16 78.2
Msa0447120 MtrunA17_Chr5g0411411 26.502 283 187 9 92 363 8 280 6.93e-16 77.8
Msa0447120 MtrunA17_Chr8g0366791 26.316 285 185 9 92 363 8 280 1.70e-15 76.6
Msa0447120 MtrunA17_Chr6g0488341 26.639 244 150 8 141 376 6 228 8.70e-15 73.6
Msa0447120 MtrunA17_Chr7g0241521 25.000 272 187 7 89 354 13 273 1.77e-14 73.6
Msa0447120 MtrunA17_Chr6g0463041 26.244 221 127 5 191 376 18 237 5.79e-14 71.2
Msa0447120 MtrunA17_Chr4g0008061 25.461 271 181 9 99 361 21 278 1.03e-13 71.2
Msa0447120 MtrunA17_Chr8g0382201 23.649 296 180 10 96 368 198 470 7.80e-13 69.7
Msa0447120 MtrunA17_Chr4g0028531 24.742 194 130 4 94 281 116 299 2.32e-11 64.3
Msa0447120 MtrunA17_Chr5g0412401 21.070 299 202 6 93 361 6 300 4.24e-11 63.5
Msa0447120 MtrunA17_Chr7g0269701 22.887 284 194 9 96 368 25 294 7.13e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0447120 AT5G13490.1 81.170 393 62 5 1 389 1 385 0.0 608
Msa0447120 AT5G13490.2 81.170 393 62 5 1 389 1 385 0.0 608
Msa0447120 AT3G08580.2 82.188 393 54 6 1 389 1 381 0.0 590
Msa0447120 AT3G08580.1 82.188 393 54 6 1 389 1 381 0.0 590
Msa0447120 AT4G28390.1 74.936 391 81 4 2 388 1 378 0.0 548
Msa0447120 AT4G28390.2 74.936 391 81 4 2 388 1 378 0.0 548
Msa0447120 AT5G17400.1 58.804 301 124 0 80 380 1 301 6.24e-130 375
Msa0447120 AT5G56450.1 38.835 309 170 7 80 376 21 322 5.24e-66 213
Msa0447120 AT3G55640.1 28.037 321 213 7 58 368 1 313 2.40e-33 127
Msa0447120 AT3G55640.4 28.037 321 213 7 58 368 1 313 2.40e-33 127
Msa0447120 AT1G14560.1 30.249 281 170 6 104 368 38 308 8.46e-33 125
Msa0447120 AT2G37890.1 30.640 297 189 7 92 379 44 332 9.51e-33 125
Msa0447120 AT1G14560.2 30.545 275 165 6 104 362 38 302 6.32e-32 123
Msa0447120 AT4G26180.1 31.270 307 177 9 89 377 17 307 9.91e-32 123
Msa0447120 AT4G26180.2 31.959 291 165 8 89 362 17 291 9.64e-31 119
Msa0447120 AT3G53940.1 29.831 295 187 8 85 368 63 348 3.27e-29 117
Msa0447120 AT3G53940.1 24.365 197 137 3 91 287 178 362 1.21e-11 65.9
Msa0447120 AT3G51870.1 32.624 282 161 9 104 379 102 360 1.50e-28 115
Msa0447120 AT4G01100.1 27.597 308 168 9 96 368 45 332 1.31e-26 109
Msa0447120 AT5G01500.1 30.824 279 170 9 104 379 130 388 1.84e-26 110
Msa0447120 AT4G32400.1 29.893 281 171 9 93 368 114 373 1.31e-25 107
Msa0447120 AT4G01100.2 27.796 313 175 9 96 368 45 346 6.85e-25 104
Msa0447120 AT4G01100.3 26.821 302 166 9 102 368 1 282 2.23e-24 102
Msa0447120 AT5G51050.1 29.825 285 167 7 93 368 223 483 1.23e-23 102
Msa0447120 AT3G21390.1 26.625 323 198 7 83 373 9 324 2.54e-23 100
Msa0447120 AT3G21390.2 26.625 323 198 7 83 373 31 346 3.22e-23 100
Msa0447120 AT5G61810.2 27.703 296 183 8 77 368 52 320 3.47e-23 99.8
Msa0447120 AT5G61810.1 27.703 296 183 8 77 368 195 463 2.73e-22 98.6
Msa0447120 AT3G55640.2 27.481 262 171 6 58 312 1 250 2.97e-22 95.5
Msa0447120 AT3G55640.3 27.481 262 171 6 58 312 1 250 2.97e-22 95.5
Msa0447120 AT1G14560.3 31.383 188 112 4 197 368 24 210 6.53e-22 94.0
Msa0447120 AT3G20240.1 24.917 301 190 6 86 368 45 327 8.86e-22 95.9
Msa0447120 AT5G48970.1 27.160 324 196 10 83 373 12 328 1.61e-21 94.7
Msa0447120 AT5G07320.1 27.778 270 169 7 102 368 218 464 6.60e-21 94.7
Msa0447120 AT3G53940.2 29.167 216 137 5 85 293 63 269 9.03e-19 86.3
Msa0447120 AT5G64970.1 27.119 295 171 11 96 368 138 410 3.85e-15 77.0
Msa0447120 AT5G48970.2 26.641 259 157 8 141 373 6 257 5.58e-15 75.1
Msa0447120 AT1G14140.1 23.507 268 190 6 93 354 16 274 1.24e-14 74.3
Msa0447120 AT1G14140.1 28.061 196 127 5 198 383 18 209 1.06e-11 65.9
Msa0447120 AT5G64970.2 26.780 295 172 11 96 368 14 286 1.72e-14 73.9
Msa0447120 AT5G64970.3 27.778 288 164 11 96 361 138 403 3.63e-14 73.9
Msa0447120 AT5G46800.2 26.573 286 186 8 92 363 7 282 6.15e-14 72.4
Msa0447120 AT5G46800.3 26.573 286 186 8 92 363 7 282 6.15e-14 72.4
Msa0447120 AT5G46800.1 26.573 286 186 8 92 363 7 282 6.15e-14 72.4
Msa0447120 AT5G58970.1 24.742 291 190 8 83 361 6 279 7.92e-14 72.0
Msa0447120 AT2G33820.1 23.404 282 201 5 89 362 14 288 3.61e-13 70.1
Msa0447120 AT5G66380.1 27.317 205 125 6 191 383 5 197 1.13e-12 68.6
Msa0447120 AT3G54110.1 23.134 268 189 8 93 354 15 271 1.35e-12 68.6
Msa0447120 AT1G79900.1 25.726 241 156 6 140 373 53 277 8.90e-12 65.9
Msa0447120 AT1G78180.1 24.415 299 174 8 96 368 131 403 9.83e-12 66.6
Msa0447120 AT2G33820.3 22.759 290 201 6 89 362 14 296 8.05e-11 63.2

Find 167 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGAAAACATGATTTATT+TGG 0.123351 3_3:+24419571 Msa0447120:three_prime_UTR
GCTGGTGCTGGTGTGCTTTC+TGG 0.149238 3_3:+24419333 Msa0447120:CDS
TGATCATCTCATCCTGGTTT+TGG 0.179358 3_3:-24418366 None:intergenic
CGGGTCTTTCATCTGGATTT+AGG 0.206368 3_3:+24418080 Msa0447120:CDS
CCATGATATGTATGCTATAT+TGG 0.212959 3_3:+24420020 Msa0447120:three_prime_UTR
AACCAAAATTTAAAAGAGTT+TGG 0.219808 3_3:-24419538 None:intergenic
GTTTATTGATTGATTGTATA+AGG 0.227083 3_3:-24419752 None:intergenic
TATTTCATGATCATCCTTAT+TGG 0.235542 3_3:+24419695 Msa0447120:three_prime_UTR
TGGCTAAATCATTAGAAAAT+TGG 0.248180 3_3:+24420114 Msa0447120:three_prime_UTR
AATGGGAGCAGCAGCAGTTT+TGG 0.270825 3_3:-24418323 None:intergenic
GGGTCTTTCATCTGGATTTA+GGG 0.271738 3_3:+24418081 Msa0447120:CDS
AACATCCTCCGTGCCGTTGC+TGG 0.301686 3_3:+24419315 Msa0447120:CDS
ACCAAAATTTAAAAGAGTTT+GGG 0.303902 3_3:-24419537 None:intergenic
AGTTCCCATAGGAGAATCTT+TGG 0.306546 3_3:-24418138 None:intergenic
TCTTCTCACTGGAAAATTGC+AGG 0.310516 3_3:+24418957 Msa0447120:CDS
TAATCACACCATTAAGAAAA+AGG 0.319564 3_3:-24419866 None:intergenic
CTTAGCTGCCTTAGAATCAT+TGG 0.323339 3_3:-24418773 None:intergenic
AGTTAGCATGACATTAGTTT+AGG 0.326894 3_3:-24419916 None:intergenic
CTTGCTACTGATGGAATTGC+TGG 0.330650 3_3:+24418847 Msa0447120:CDS
AGAAGGACAGGGATGGTTAC+TGG 0.330831 3_3:+24418656 Msa0447120:CDS
TTCTAATGATTTAGCCATCT+TGG 0.333491 3_3:-24420108 None:intergenic
CCTCATTCTTGAGGATTTGT+TGG 0.335787 3_3:-24419260 None:intergenic
TCTAGTTAAGAAAACCAAAA+AGG 0.338031 3_3:+24419950 Msa0447120:three_prime_UTR
CTCCGTGCCGTTGCTGGTGC+TGG 0.343034 3_3:+24419321 Msa0447120:CDS
GTATGAAAATTGTCTAAAAT+TGG 0.343683 3_3:+24420170 Msa0447120:three_prime_UTR
GAGCCATCCAAGTGCAAAAC+TGG 0.358699 3_3:-24419130 None:intergenic
CGTCGATCGGGTCTTTCATC+TGG 0.364825 3_3:+24418073 Msa0447120:CDS
GAGCAAGATTCTAACCAATA+AGG 0.365498 3_3:-24419709 None:intergenic
TTTCATATGGAAAATGGAAA+TGG 0.368498 3_3:-24420264 None:intergenic
ACAATCACCAATACCCTTGT+AGG 0.369255 3_3:-24418410 None:intergenic
TAAACTAATGTCATGCTAAC+TGG 0.375168 3_3:+24419918 Msa0447120:three_prime_UTR
TTGGACATTAATTTATTTGA+GGG 0.379854 3_3:+24420133 Msa0447120:three_prime_UTR
GTGACCTTCTCCATGATTGT+TGG 0.389407 3_3:-24418037 None:intergenic
CGTTCTACGACAAATATTAC+TGG 0.391240 3_3:-24420060 None:intergenic
CAAAAGCTTAATACGTTCAA+TGG 0.391915 3_3:-24418341 None:intergenic
TCGTCTTGCCAATGATTCTA+AGG 0.397580 3_3:+24418765 Msa0447120:CDS
ACAGGGATGGTTACTGGAAA+TGG 0.399761 3_3:+24418662 Msa0447120:CDS
TGTGTAGTCAAAGTATAAAA+TGG 0.401063 3_3:-24419993 None:intergenic
CATGATTTATTTGGAGTGGC+AGG 0.411321 3_3:+24419580 Msa0447120:three_prime_UTR
TTGGCATCCGGAGGTGCTGC+TGG 0.411978 3_3:+24418697 Msa0447120:CDS
TTCTGGTTACGACAAGTTGC+AGG 0.414142 3_3:+24419350 Msa0447120:CDS
GAAAGCAACCATGGTTGATC+AGG 0.420030 3_3:+24418006 Msa0447120:exon
CTTCTCCTAACAGTGTCAAT+GGG 0.420342 3_3:-24419183 None:intergenic
TGAGGCACCAGCAGCACCTC+CGG 0.430152 3_3:-24418704 None:intergenic
GTAGAACGATGAACAATTAC+TGG 0.430563 3_3:+24420075 Msa0447120:three_prime_UTR
TTTCGTTATTGACTTTCTCA+TGG 0.438686 3_3:+24418279 Msa0447120:CDS
CTTGTGTTCGGCAAGAAATA+CGG 0.440747 3_3:+24419375 Msa0447120:CDS
GACCCGATCGACGGTGGAGC+TGG 0.442042 3_3:-24418063 None:intergenic
AAGTTGCAGGTTCTTGTGTT+CGG 0.444616 3_3:+24419363 Msa0447120:CDS
ATTGGACATTAATTTATTTG+AGG 0.444738 3_3:+24420132 Msa0447120:three_prime_UTR
CTTGTACTTGACAGCTTCTC+CGG 0.448393 3_3:-24419220 None:intergenic
GGCCAGCTCCACCGTCGATC+GGG 0.448817 3_3:+24418061 Msa0447120:CDS
TATTACTGGCATGATAGTTA+AGG 0.449225 3_3:-24420046 None:intergenic
AGTGAGAAGAACTGGTTTGA+GGG 0.449368 3_3:-24418944 None:intergenic
CCAATATAGCATACATATCA+TGG 0.451061 3_3:-24420020 None:intergenic
CAGTGAGAAGAACTGGTTTG+AGG 0.452118 3_3:-24418945 None:intergenic
GGTTACTGGAAATGGTTTGC+CGG 0.452634 3_3:+24418670 Msa0447120:CDS
TCTTCTCCTAACAGTGTCAA+TGG 0.452687 3_3:-24419184 None:intergenic
GAAATGGTTTGCCGGTAACT+TGG 0.453917 3_3:+24418678 Msa0447120:CDS
ACCTCCGGATGCCAAGTTAC+CGG 0.453929 3_3:-24418689 None:intergenic
AAATACCAAATGAATAAAAC+AGG 0.456337 3_3:-24419892 None:intergenic
TGAGAAAGTCAATAACGAAA+TGG 0.456385 3_3:-24418276 None:intergenic
CTCAAACCAGTTCTTCTCAC+TGG 0.459621 3_3:+24418946 Msa0447120:CDS
CACCAGCACCAGCAACGGCA+CGG 0.468876 3_3:-24419323 None:intergenic
GTTCCCATAGGAGAATCTTT+GGG 0.471265 3_3:-24418137 None:intergenic
CATACCCCATTGACACTGTT+AGG 0.473018 3_3:+24419178 Msa0447120:CDS
TGGCCAGCTCCACCGTCGAT+CGG 0.474097 3_3:+24418060 Msa0447120:CDS
GCTGGAGCAGCGACGAAGAC+AGG 0.475793 3_3:-24418244 None:intergenic
TCTGGATTTAGGGGTTATGA+AGG 0.477435 3_3:+24418091 Msa0447120:CDS
ATCATTGGCAAGACGAGTAC+GGG 0.477887 3_3:-24418758 None:intergenic
AGAAAATGGCTAACCTGAGT+AGG 0.478242 3_3:-24418508 None:intergenic
ATGAGGGTATTGTTGCTCTC+TGG 0.479479 3_3:+24418452 Msa0447120:CDS
AACACCTACTATCATCATAC+TGG 0.479860 3_3:-24419779 None:intergenic
ATCAACAGTTGCCCTGCAAG+TGG 0.483410 3_3:-24418194 None:intergenic
TCAATCAACGAAGACAGGCC+AGG 0.487277 3_3:-24420290 None:intergenic
AAAAGCTTAATACGTTCAAT+GGG 0.489695 3_3:-24418340 None:intergenic
TCTGAGCCCTACAAGGGTAT+TGG 0.490496 3_3:+24418403 Msa0447120:CDS
AATACCAGTATGATGATAGT+AGG 0.491772 3_3:+24419775 Msa0447120:three_prime_UTR
ATGTCCCAAAGATTCTCCTA+TGG 0.492338 3_3:+24418133 Msa0447120:CDS
GGTTTCAACATCTCCTGTGT+TGG 0.492793 3_3:+24418880 Msa0447120:CDS
TTCGTTATTGACTTTCTCAT+GGG 0.493555 3_3:+24418280 Msa0447120:CDS
CCAACAAATCCTCAAGAATG+AGG 0.494163 3_3:+24419260 Msa0447120:CDS
CAGGATGAGATGATCAAGTC+TGG 0.496357 3_3:+24418373 Msa0447120:CDS
CCATACACGTTTCAGAGGCC+AGG 0.498210 3_3:-24420373 None:intergenic
AGAAAATTAGTCACAAGCAC+AGG 0.500935 3_3:-24420223 None:intergenic
AAGCACACCAGCACCAGCAA+CGG 0.501311 3_3:-24419328 None:intergenic
GGGTGCTAAGTCATTGTTCA+AGG 0.502290 3_3:+24419281 Msa0447120:CDS
AATCATTGGCAAGACGAGTA+CGG 0.503375 3_3:-24418759 None:intergenic
AAGAAGACTCTTGCTACTGA+TGG 0.508472 3_3:+24418838 Msa0447120:CDS
TTAGTCACAAGCACAGGTTA+AGG 0.513970 3_3:-24420217 None:intergenic
ACAATCATGGAGAAGGTCAC+TGG 0.514525 3_3:+24418040 Msa0447120:CDS
GTTTAATAAATTTACTCGGT+GGG 0.517965 3_3:+24419507 Msa0447120:three_prime_UTR
GTTTACCGTGGTCTGTACTT+CGG 0.519266 3_3:+24418910 Msa0447120:CDS
TTCGGCAAGAAATACGGATC+TGG 0.519522 3_3:+24419381 Msa0447120:CDS
CAGACTTGATCATCTCATCC+TGG 0.520517 3_3:-24418372 None:intergenic
AAGTCATTGTTCAAGGGAGC+TGG 0.521554 3_3:+24419288 Msa0447120:CDS
TTTGCCGGTAACTTGGCATC+CGG 0.521620 3_3:+24418685 Msa0447120:CDS
GGTTAAGCAACCAACAATCA+TGG 0.524656 3_3:+24418027 Msa0447120:CDS
TGATGCTAGACCTGCACCAT+TGG 0.527310 3_3:-24419157 None:intergenic
AGAAGAATGATGATGACATC+CGG 0.527583 3_3:+24419201 Msa0447120:CDS
CTTTGTCAATCAACGAAGAC+AGG 0.528211 3_3:-24420295 None:intergenic
CAACAGTTGCCCTGCAAGTG+GGG 0.528651 3_3:-24418192 None:intergenic
TGGCTCATTACCAATGGTGC+AGG 0.529441 3_3:+24419147 Msa0447120:CDS
TATCCGTTATTTCCCTACTC+AGG 0.534487 3_3:+24418495 Msa0447120:CDS
TAACCTGAGTAGGGAAATAA+CGG 0.535034 3_3:-24418498 None:intergenic
CATTCAAAGATTACTTCAAG+AGG 0.536246 3_3:+24418620 Msa0447120:CDS
TAGGCTATCTGAGCCCTACA+AGG 0.536940 3_3:+24418396 Msa0447120:CDS
GATTCTAAGGCAGCTAAGAA+AGG 0.539062 3_3:+24418778 Msa0447120:CDS
CAGCTAAGAAAGGTGGAGAA+AGG 0.539801 3_3:+24418788 Msa0447120:CDS
AGCACAGGTTAAGGAGATGA+AGG 0.542530 3_3:-24420208 None:intergenic
CAGGATAAGTAACTGTCACT+TGG 0.543016 3_3:+24419599 Msa0447120:three_prime_UTR
GAAAATGGCTAACCTGAGTA+GGG 0.543044 3_3:-24418507 None:intergenic
GAGAATCTTTGGGACATGGC+AGG 0.547914 3_3:-24418127 None:intergenic
GTTATTGACTTTCTCATGGG+TGG 0.552168 3_3:+24418283 Msa0447120:CDS
TTGCTACTGATGGAATTGCT+GGG 0.552525 3_3:+24418848 Msa0447120:CDS
TGATCATGAAATAAAACTCT+AGG 0.552839 3_3:-24419685 None:intergenic
ACTTAGCACCCTCATTCTTG+AGG 0.554699 3_3:-24419269 None:intergenic
GCCGGTAACTTGGCATCCGG+AGG 0.555811 3_3:+24418688 Msa0447120:CDS
TCAACAGTTGCCCTGCAAGT+GGG 0.556580 3_3:-24418193 None:intergenic
AAAACATGATTTATTTGGAG+TGG 0.558044 3_3:+24419576 Msa0447120:three_prime_UTR
AAAAGAACAATGGCCGATGA+GGG 0.560661 3_3:+24418436 Msa0447120:CDS
GGACATGGCAGGATGGCGGT+AGG 0.563819 3_3:-24418116 None:intergenic
TGTCCCAAAGATTCTCCTAT+GGG 0.564873 3_3:+24418134 Msa0447120:CDS
GATTTGTTGGAATGCATCAA+AGG 0.565548 3_3:-24419247 None:intergenic
GGTGGAGAAAGGCAGTTCAA+TGG 0.572074 3_3:+24418799 Msa0447120:CDS
TAAAAGAACAATGGCCGATG+AGG 0.576304 3_3:+24418435 Msa0447120:CDS
GGTCTTTCATCTGGATTTAG+GGG 0.580823 3_3:+24418082 Msa0447120:CDS
CAATCACCAATACCCTTGTA+GGG 0.581476 3_3:-24418409 None:intergenic
GAAGTGTTACCTTTCACGAA+GGG 0.581786 3_3:-24416868 None:intergenic
AACAGTTGCCCTGCAAGTGG+GGG 0.584275 3_3:-24418191 None:intergenic
GAGAGCAACAATACCCTCAT+CGG 0.586821 3_3:-24418449 None:intergenic
AGAGAGAGAGATGAGTGTGG+AGG 0.588109 3_3:-24416808 None:intergenic
TGTTTAATAAATTTACTCGG+TGG 0.590627 3_3:+24419506 Msa0447120:three_prime_UTR
TGAAGTGTTACCTTTCACGA+AGG 0.592018 3_3:-24416869 None:intergenic
ATGAGATGATCAAGTCTGGT+AGG 0.599652 3_3:+24418377 Msa0447120:CDS
CGTAGCCATACACGTTTCAG+AGG 0.599767 3_3:-24420378 None:intergenic
CAAGAGAGTAAACAAACAAG+AGG 0.602030 3_3:-24418729 None:intergenic
CAGATGAAAGACCCGATCGA+CGG 0.603535 3_3:-24418072 None:intergenic
ATCTTTGGGACATGGCAGGA+TGG 0.604045 3_3:-24418123 None:intergenic
AACTTCAAGAAGGACAGGGA+TGG 0.606997 3_3:+24418649 Msa0447120:CDS
CGCTGCATTGCAGAATCCAA+TGG 0.607456 3_3:+24418168 Msa0447120:CDS
CAACAAATCCTCAAGAATGA+GGG 0.607876 3_3:+24419261 Msa0447120:CDS
ATGAAAGACCCGATCGACGG+TGG 0.608954 3_3:-24418069 None:intergenic
GGTATTGTTGCTCTCTGGAG+AGG 0.610023 3_3:+24418457 Msa0447120:CDS
AGTATGATGATAGTAGGTGT+TGG 0.622229 3_3:+24419781 Msa0447120:three_prime_UTR
GCAACCAACAATCATGGAGA+AGG 0.628211 3_3:+24418033 Msa0447120:CDS
GGCAAGAAATACGGATCTGG+TGG 0.628657 3_3:+24419384 Msa0447120:CDS
TCTAAGGCAGCTAAGAAAGG+TGG 0.629619 3_3:+24418781 Msa0447120:CDS
GTAAACAAACAAGAGGGATG+AGG 0.631365 3_3:-24418722 None:intergenic
CACAGGAGATGTTGAAACCA+CGG 0.633206 3_3:-24418876 None:intergenic
ATAGGAGAATCTTTGGGACA+TGG 0.635000 3_3:-24418131 None:intergenic
TTTCAACTTCAAGAAGGACA+GGG 0.637221 3_3:+24418645 Msa0447120:CDS
GTAAACAATGATTCCAACAC+AGG 0.637438 3_3:-24418893 None:intergenic
CTTGGATGGCTCATTACCAA+TGG 0.637848 3_3:+24419141 Msa0447120:CDS
AGGGAAATAACGGATAACAT+TGG 0.640612 3_3:-24418488 None:intergenic
GGTGCTAAGTCATTGTTCAA+GGG 0.642143 3_3:+24419282 Msa0447120:CDS
GCAGCAATTAGTCTAACTCT+CGG 0.646966 3_3:-24419474 None:intergenic
GGAATTGCTGGGCTTTACCG+TGG 0.654046 3_3:+24418859 Msa0447120:CDS
AAGAGAGTAAACAAACAAGA+GGG 0.655445 3_3:-24418728 None:intergenic
AGCGTTTGAGTAGTTCCCAT+AGG 0.663946 3_3:-24418149 None:intergenic
CAGCACCAGCAACGGCACGG+AGG 0.668727 3_3:-24419320 None:intergenic
TTGCTTAACCTGATCAACCA+TGG 0.675233 3_3:-24418014 None:intergenic
TTTGGGACATGGCAGGATGG+CGG 0.679753 3_3:-24418120 None:intergenic
TTCTCCTAACAGTGTCAATG+GGG 0.682740 3_3:-24419182 None:intergenic
GTTGGAATCATTGTTTACCG+TGG 0.683302 3_3:+24418898 Msa0447120:CDS
AGGCTATCTGAGCCCTACAA+GGG 0.684234 3_3:+24418397 Msa0447120:CDS
ACAATCCGAAGTACAGACCA+CGG 0.718426 3_3:-24418915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTTGAAGATTATAT+TGG + chr3_3:24417899-24417918 Msa0447120:intron 10.0%
!! ATGATAATATAAATAAACTA+AGG - chr3_3:24417942-24417961 None:intergenic 10.0%
!!! TTTTGTTTAATAAATTTACT+CGG + chr3_3:24419503-24419522 Msa0447120:three_prime_UTR 10.0%
!!! TAATATAAAAATTTCCTTTT+TGG - chr3_3:24419967-24419986 None:intergenic 10.0%
!! TAAAATTGAAAACTAGAAAA+TGG - chr3_3:24418525-24418544 None:intergenic 15.0%
!!! TCAAATTTATTGTTTTTAGA+TGG + chr3_3:24419431-24419450 Msa0447120:three_prime_UTR 15.0%
!! AATTGAAAACATGATTTATT+TGG + chr3_3:24419571-24419590 Msa0447120:three_prime_UTR 15.0%
!!! TGTTATATCTTGATTTTATA+TGG + chr3_3:24419826-24419845 Msa0447120:three_prime_UTR 15.0%
!!! GTTATATCTTGATTTTATAT+GGG + chr3_3:24419827-24419846 Msa0447120:three_prime_UTR 15.0%
!!! TTATATCTTGATTTTATATG+GGG + chr3_3:24419828-24419847 Msa0447120:three_prime_UTR 15.0%
!!! TTGAATTTTTATTCGATTTG+AGG + chr3_3:24417008-24417027 Msa0447120:intron 20.0%
!!! GAGGTTTTTTTTTTAATTTG+TGG + chr3_3:24417027-24417046 Msa0447120:intron 20.0%
!! TTTGAAGATTATATTGGATA+TGG + chr3_3:24417905-24417924 Msa0447120:intron 20.0%
!!! TGATTGTTTTAAAAGAACAA+TGG + chr3_3:24418426-24418445 Msa0447120:CDS 20.0%
!! CATTAAATACACAAAACAAT+TGG - chr3_3:24418555-24418574 None:intergenic 20.0%
!! ATGAATATCATTTGTTCAAA+GGG - chr3_3:24418995-24419014 None:intergenic 20.0%
!! AATGAATATCATTTGTTCAA+AGG - chr3_3:24418996-24419015 None:intergenic 20.0%
!! TGCAACGAAAAATAATAATT+TGG - chr3_3:24419023-24419042 None:intergenic 20.0%
!! ACCAAAATTTAAAAGAGTTT+GGG - chr3_3:24419540-24419559 None:intergenic 20.0%
!! AACCAAAATTTAAAAGAGTT+TGG - chr3_3:24419541-24419560 None:intergenic 20.0%
!!! AAACTCTTTTAAATTTTGGT+TGG + chr3_3:24419540-24419559 Msa0447120:three_prime_UTR 20.0%
!!! GTTTATTGATTGATTGTATA+AGG - chr3_3:24419755-24419774 None:intergenic 20.0%
!! AAATACCAAATGAATAAAAC+AGG - chr3_3:24419895-24419914 None:intergenic 20.0%
!! ATTGGACATTAATTTATTTG+AGG + chr3_3:24420132-24420151 Msa0447120:three_prime_UTR 20.0%
!! TTGGACATTAATTTATTTGA+GGG + chr3_3:24420133-24420152 Msa0447120:three_prime_UTR 20.0%
!!! GTATGAAAATTGTCTAAAAT+TGG + chr3_3:24420170-24420189 Msa0447120:three_prime_UTR 20.0%
!!! TACTGTTGATTTGATTCATA+CGG + chr3_3:24416963-24416982 Msa0447120:intron 25.0%
!! ATTCATACGGTTTTTCATTT+TGG + chr3_3:24416976-24416995 Msa0447120:intron 25.0%
! ACAGAATTGTAACTACTAAT+TGG - chr3_3:24417284-24417303 None:intergenic 25.0%
! TTGTCTGTATATAAAACTTG+TGG + chr3_3:24417334-24417353 Msa0447120:intron 25.0%
! AAGATTTGTAAACTGAATTG+TGG - chr3_3:24417407-24417426 None:intergenic 25.0%
! ACAATTCAGTTTACAAATCT+TGG + chr3_3:24417406-24417425 Msa0447120:intron 25.0%
! ACTAAATTATCTTCCAGAAA+AGG - chr3_3:24417472-24417491 None:intergenic 25.0%
!! ATTTTGTGATCTAGATAAGT+AGG + chr3_3:24417506-24417525 Msa0447120:intron 25.0%
!! TTTTGTGATCTAGATAAGTA+GGG + chr3_3:24417507-24417526 Msa0447120:intron 25.0%
! CATTGAATAATTGATGTATC+TGG - chr3_3:24417628-24417647 None:intergenic 25.0%
! AAATTGATCAAAGACTACAT+CGG - chr3_3:24417684-24417703 None:intergenic 25.0%
! CTTAATACTTCAGATTATGA+AGG + chr3_3:24417769-24417788 Msa0447120:intron 25.0%
!! TATTGGATATGGATTGATTA+TGG + chr3_3:24417916-24417935 Msa0447120:intron 25.0%
! TAATCGACTAATGTCAAATA+AGG - chr3_3:24417973-24417992 None:intergenic 25.0%
!!! AGTCGATTACATTTTTTTTC+AGG + chr3_3:24417984-24418003 Msa0447120:intron 25.0%
! AAAAGCTTAATACGTTCAAT+GGG - chr3_3:24418343-24418362 None:intergenic 25.0%
!! TCTGCTATTTAAATTGTTCT+AGG + chr3_3:24418585-24418604 Msa0447120:intron 25.0%
!! TTTTGAATTCTTGACATTAC+AGG + chr3_3:24419095-24419114 Msa0447120:intron 25.0%
! TGTTTAATAAATTTACTCGG+TGG + chr3_3:24419506-24419525 Msa0447120:three_prime_UTR 25.0%
! GTTTAATAAATTTACTCGGT+GGG + chr3_3:24419507-24419526 Msa0447120:three_prime_UTR 25.0%
!! TCCCAAACTCTTTTAAATTT+TGG + chr3_3:24419536-24419555 Msa0447120:three_prime_UTR 25.0%
! AAAACATGATTTATTTGGAG+TGG + chr3_3:24419576-24419595 Msa0447120:three_prime_UTR 25.0%
! TGATCATGAAATAAAACTCT+AGG - chr3_3:24419688-24419707 None:intergenic 25.0%
! TATTTCATGATCATCCTTAT+TGG + chr3_3:24419695-24419714 Msa0447120:three_prime_UTR 25.0%
! TAATCACACCATTAAGAAAA+AGG - chr3_3:24419869-24419888 None:intergenic 25.0%
!! TTTTCTTAATGGTGTGATTA+TGG + chr3_3:24419869-24419888 Msa0447120:three_prime_UTR 25.0%
!! TGGTTTTCTTAACTAGAAAA+CGG - chr3_3:24419947-24419966 None:intergenic 25.0%
! TCTAGTTAAGAAAACCAAAA+AGG + chr3_3:24419950-24419969 Msa0447120:three_prime_UTR 25.0%
! TGTGTAGTCAAAGTATAAAA+TGG - chr3_3:24419996-24420015 None:intergenic 25.0%
! TGGCTAAATCATTAGAAAAT+TGG + chr3_3:24420114-24420133 Msa0447120:three_prime_UTR 25.0%
! TTTCATATGGAAAATGGAAA+TGG - chr3_3:24420267-24420286 None:intergenic 25.0%
!! AGGATTTTTCATATGGAAAA+TGG - chr3_3:24420273-24420292 None:intergenic 25.0%
!! ATTTTCCATATGAAAAATCC+TGG + chr3_3:24420272-24420291 Msa0447120:three_prime_UTR 25.0%
! ACAAAGTAAAGTAAAAATCC+TGG + chr3_3:24420355-24420374 Msa0447120:three_prime_UTR 25.0%
!! TGTGATTGGATCTTAGTTTT+TGG + chr3_3:24417173-24417192 Msa0447120:intron 30.0%
AGTAGTTACAATTCTGTACT+TGG + chr3_3:24417287-24417306 Msa0447120:intron 30.0%
TAAAACTTGTGGTTCATGTT+TGG + chr3_3:24417345-24417364 Msa0447120:intron 30.0%
!!! TTGGTTCTGTTAGTATTTTG+TGG + chr3_3:24417425-24417444 Msa0447120:intron 30.0%
!!! TTTTGTGGCTTTAGAATTTG+TGG + chr3_3:24417440-24417459 Msa0447120:intron 30.0%
!! TTTGTGGATTATACCTTTTC+TGG + chr3_3:24417456-24417475 Msa0447120:intron 30.0%
AATTGATCAAAGACTACATC+GGG - chr3_3:24417683-24417702 None:intergenic 30.0%
TGAGAAAGTCAATAACGAAA+TGG - chr3_3:24418279-24418298 None:intergenic 30.0%
!! TTTCGTTATTGACTTTCTCA+TGG + chr3_3:24418279-24418298 Msa0447120:CDS 30.0%
!! TTCGTTATTGACTTTCTCAT+GGG + chr3_3:24418280-24418299 Msa0447120:CDS 30.0%
CAAAAGCTTAATACGTTCAA+TGG - chr3_3:24418344-24418363 None:intergenic 30.0%
CATTCAAAGATTACTTCAAG+AGG + chr3_3:24418620-24418639 Msa0447120:CDS 30.0%
AAGAGAGTAAACAAACAAGA+GGG - chr3_3:24418731-24418750 None:intergenic 30.0%
AATACCAGTATGATGATAGT+AGG + chr3_3:24419775-24419794 Msa0447120:three_prime_UTR 30.0%
! ATCTTGATTTTATATGGGGA+TGG + chr3_3:24419832-24419851 Msa0447120:three_prime_UTR 30.0%
!! TATGGCCTGTTTTATTCATT+TGG + chr3_3:24419887-24419906 Msa0447120:three_prime_UTR 30.0%
! AGTTAGCATGACATTAGTTT+AGG - chr3_3:24419919-24419938 None:intergenic 30.0%
TAAACTAATGTCATGCTAAC+TGG + chr3_3:24419918-24419937 Msa0447120:three_prime_UTR 30.0%
! GGTTTTCTTAACTAGAAAAC+GGG - chr3_3:24419946-24419965 None:intergenic 30.0%
CCAATATAGCATACATATCA+TGG - chr3_3:24420023-24420042 None:intergenic 30.0%
CCATGATATGTATGCTATAT+TGG + chr3_3:24420020-24420039 Msa0447120:three_prime_UTR 30.0%
TATTACTGGCATGATAGTTA+AGG - chr3_3:24420049-24420068 None:intergenic 30.0%
TTCTAATGATTTAGCCATCT+TGG - chr3_3:24420111-24420130 None:intergenic 30.0%
ACTGTTTGTGTTTGTCTGTT+CGG + chr3_3:24417114-24417133 Msa0447120:intron 35.0%
! TCAGATTGTTTCTCTGTGAT+TGG + chr3_3:24417159-24417178 Msa0447120:intron 35.0%
! CTGTTGGTTGATTCTTTAGA+TGG + chr3_3:24417542-24417561 Msa0447120:intron 35.0%
CGTTGTGTGATTGATGTATA+TGG + chr3_3:24417589-24417608 Msa0447120:intron 35.0%
!!! TTTTTTCAGGAAAGCAACCA+TGG + chr3_3:24417997-24418016 Msa0447120:intron 35.0%
! TAAGCTTTTGATCCAAAACC+AGG + chr3_3:24418354-24418373 Msa0447120:CDS 35.0%
!! AGGGAAATAACGGATAACAT+TGG - chr3_3:24418491-24418510 None:intergenic 35.0%
TAACCTGAGTAGGGAAATAA+CGG - chr3_3:24418501-24418520 None:intergenic 35.0%
! TTTTCAACTTCAAGAAGGAC+AGG + chr3_3:24418644-24418663 Msa0447120:CDS 35.0%
TTTCAACTTCAAGAAGGACA+GGG + chr3_3:24418645-24418664 Msa0447120:CDS 35.0%
CAAGAGAGTAAACAAACAAG+AGG - chr3_3:24418732-24418751 None:intergenic 35.0%
GTAAACAATGATTCCAACAC+AGG - chr3_3:24418896-24418915 None:intergenic 35.0%
! AATTTTCCAGTGAGAAGAAC+TGG - chr3_3:24418955-24418974 None:intergenic 35.0%
! AGATCAAAAGTATGTTGCAG+TGG - chr3_3:24419052-24419071 None:intergenic 35.0%
! AGAAGAATGATGATGACATC+CGG + chr3_3:24419201-24419220 Msa0447120:CDS 35.0%
!! GATTTGTTGGAATGCATCAA+AGG - chr3_3:24419250-24419269 None:intergenic 35.0%
CAACAAATCCTCAAGAATGA+GGG + chr3_3:24419261-24419280 Msa0447120:CDS 35.0%
GAGCAAGATTCTAACCAATA+AGG - chr3_3:24419712-24419731 None:intergenic 35.0%
AACACCTACTATCATCATAC+TGG - chr3_3:24419782-24419801 None:intergenic 35.0%
AGTATGATGATAGTAGGTGT+TGG + chr3_3:24419781-24419800 Msa0447120:three_prime_UTR 35.0%
! GGTTCATGCCTTTTTCTTAA+TGG + chr3_3:24419858-24419877 Msa0447120:three_prime_UTR 35.0%
CGTTCTACGACAAATATTAC+TGG - chr3_3:24420063-24420082 None:intergenic 35.0%
GTAGAACGATGAACAATTAC+TGG + chr3_3:24420075-24420094 Msa0447120:three_prime_UTR 35.0%
AGAAAATTAGTCACAAGCAC+AGG - chr3_3:24420226-24420245 None:intergenic 35.0%
! GAAGTGTTACCTTTCACGAA+GGG - chr3_3:24416871-24416890 None:intergenic 40.0%
! TGAAGTGTTACCTTTCACGA+AGG - chr3_3:24416872-24416891 None:intergenic 40.0%
TTTGTCTGTTCGGATCTGAT+TGG + chr3_3:24417124-24417143 Msa0447120:intron 40.0%
AGGGTTATTCATGTAGCTGT+TGG + chr3_3:24417526-24417545 Msa0447120:intron 40.0%
TTGCTTAACCTGATCAACCA+TGG - chr3_3:24418017-24418036 None:intergenic 40.0%
GGTTAAGCAACCAACAATCA+TGG + chr3_3:24418027-24418046 Msa0447120:CDS 40.0%
GGGTCTTTCATCTGGATTTA+GGG + chr3_3:24418081-24418100 Msa0447120:CDS 40.0%
GGTCTTTCATCTGGATTTAG+GGG + chr3_3:24418082-24418101 Msa0447120:CDS 40.0%
TCTGGATTTAGGGGTTATGA+AGG + chr3_3:24418091-24418110 Msa0447120:CDS 40.0%
!! ATAGGAGAATCTTTGGGACA+TGG - chr3_3:24418134-24418153 None:intergenic 40.0%
ATGTCCCAAAGATTCTCCTA+TGG + chr3_3:24418133-24418152 Msa0447120:CDS 40.0%
TGTCCCAAAGATTCTCCTAT+GGG + chr3_3:24418134-24418153 Msa0447120:CDS 40.0%
GTTCCCATAGGAGAATCTTT+GGG - chr3_3:24418140-24418159 None:intergenic 40.0%
AGTTCCCATAGGAGAATCTT+TGG - chr3_3:24418141-24418160 None:intergenic 40.0%
!! GTTATTGACTTTCTCATGGG+TGG + chr3_3:24418283-24418302 Msa0447120:CDS 40.0%
! TGATCATCTCATCCTGGTTT+TGG - chr3_3:24418369-24418388 None:intergenic 40.0%
ATGAGATGATCAAGTCTGGT+AGG + chr3_3:24418377-24418396 Msa0447120:CDS 40.0%
CAATCACCAATACCCTTGTA+GGG - chr3_3:24418412-24418431 None:intergenic 40.0%
ACAATCACCAATACCCTTGT+AGG - chr3_3:24418413-24418432 None:intergenic 40.0%
TAAAAGAACAATGGCCGATG+AGG + chr3_3:24418435-24418454 Msa0447120:CDS 40.0%
AAAAGAACAATGGCCGATGA+GGG + chr3_3:24418436-24418455 Msa0447120:CDS 40.0%
! TATCCGTTATTTCCCTACTC+AGG + chr3_3:24418495-24418514 Msa0447120:CDS 40.0%
GAAAATGGCTAACCTGAGTA+GGG - chr3_3:24418510-24418529 None:intergenic 40.0%
AGAAAATGGCTAACCTGAGT+AGG - chr3_3:24418511-24418530 None:intergenic 40.0%
! GAGGCTTTTCAACTTCAAGA+AGG + chr3_3:24418639-24418658 Msa0447120:CDS 40.0%
GTAAACAAACAAGAGGGATG+AGG - chr3_3:24418725-24418744 None:intergenic 40.0%
AATCATTGGCAAGACGAGTA+CGG - chr3_3:24418762-24418781 None:intergenic 40.0%
TCGTCTTGCCAATGATTCTA+AGG + chr3_3:24418765-24418784 Msa0447120:CDS 40.0%
CTTAGCTGCCTTAGAATCAT+TGG - chr3_3:24418776-24418795 None:intergenic 40.0%
!! GATTCTAAGGCAGCTAAGAA+AGG + chr3_3:24418778-24418797 Msa0447120:CDS 40.0%
AAGAAGACTCTTGCTACTGA+TGG + chr3_3:24418838-24418857 Msa0447120:CDS 40.0%
TTGCTACTGATGGAATTGCT+GGG + chr3_3:24418848-24418867 Msa0447120:CDS 40.0%
GTTGGAATCATTGTTTACCG+TGG + chr3_3:24418898-24418917 Msa0447120:CDS 40.0%
!! AGTGAGAAGAACTGGTTTGA+GGG - chr3_3:24418947-24418966 None:intergenic 40.0%
TCTTCTCACTGGAAAATTGC+AGG + chr3_3:24418957-24418976 Msa0447120:CDS 40.0%
! TTTTTCGCCAGTTTTGCACT+TGG + chr3_3:24419123-24419142 Msa0447120:CDS 40.0%
TTCTCCTAACAGTGTCAATG+GGG - chr3_3:24419185-24419204 None:intergenic 40.0%
CTTCTCCTAACAGTGTCAAT+GGG - chr3_3:24419186-24419205 None:intergenic 40.0%
TCTTCTCCTAACAGTGTCAA+TGG - chr3_3:24419187-24419206 None:intergenic 40.0%
CCTCATTCTTGAGGATTTGT+TGG - chr3_3:24419263-24419282 None:intergenic 40.0%
CCAACAAATCCTCAAGAATG+AGG + chr3_3:24419260-24419279 Msa0447120:CDS 40.0%
GGTGCTAAGTCATTGTTCAA+GGG + chr3_3:24419282-24419301 Msa0447120:CDS 40.0%
AAGTTGCAGGTTCTTGTGTT+CGG + chr3_3:24419363-24419382 Msa0447120:CDS 40.0%
CTTGTGTTCGGCAAGAAATA+CGG + chr3_3:24419375-24419394 Msa0447120:CDS 40.0%
! GCAGCAATTAGTCTAACTCT+CGG - chr3_3:24419477-24419496 None:intergenic 40.0%
! CATGATTTATTTGGAGTGGC+AGG + chr3_3:24419580-24419599 Msa0447120:three_prime_UTR 40.0%
CAGGATAAGTAACTGTCACT+TGG + chr3_3:24419599-24419618 Msa0447120:three_prime_UTR 40.0%
! GATTTTATATGGGGATGGTG+TGG + chr3_3:24419837-24419856 Msa0447120:three_prime_UTR 40.0%
! CTGGTGAATTTTCTCCAAGA+TGG + chr3_3:24420094-24420113 Msa0447120:three_prime_UTR 40.0%
TTAGTCACAAGCACAGGTTA+AGG - chr3_3:24420220-24420239 None:intergenic 40.0%
! ACAGGCCAGGATTTTTCATA+TGG - chr3_3:24420280-24420299 None:intergenic 40.0%
CTTTGTCAATCAACGAAGAC+AGG - chr3_3:24420298-24420317 None:intergenic 40.0%
! TCACGAAGGGTTTTTGCGAA+AGG - chr3_3:24416858-24416877 None:intergenic 45.0%
TCGCAAAAACCCTTCGTGAA+AGG + chr3_3:24416859-24416878 Msa0447120:five_prime_UTR 45.0%
GTAGTACTCTCTCTAGTCGT+TGG + chr3_3:24417564-24417583 Msa0447120:intron 45.0%
GAAAGCAACCATGGTTGATC+AGG + chr3_3:24418006-24418025 Msa0447120:exon 45.0%
GCAACCAACAATCATGGAGA+AGG + chr3_3:24418033-24418052 Msa0447120:CDS 45.0%
GTGACCTTCTCCATGATTGT+TGG - chr3_3:24418040-24418059 None:intergenic 45.0%
ACAATCATGGAGAAGGTCAC+TGG + chr3_3:24418040-24418059 Msa0447120:CDS 45.0%
CGGGTCTTTCATCTGGATTT+AGG + chr3_3:24418080-24418099 Msa0447120:CDS 45.0%
AGCGTTTGAGTAGTTCCCAT+AGG - chr3_3:24418152-24418171 None:intergenic 45.0%
! ACGAAATGGCTCTTTTCTGC+TGG - chr3_3:24418265-24418284 None:intergenic 45.0%
!!! AGCAGCAGCAGTTTTGGAAA+CGG - chr3_3:24418320-24418339 None:intergenic 45.0%
CAGACTTGATCATCTCATCC+TGG - chr3_3:24418375-24418394 None:intergenic 45.0%
CAGGATGAGATGATCAAGTC+TGG + chr3_3:24418373-24418392 Msa0447120:CDS 45.0%
GAGAGCAACAATACCCTCAT+CGG - chr3_3:24418452-24418471 None:intergenic 45.0%
!! ATGAGGGTATTGTTGCTCTC+TGG + chr3_3:24418452-24418471 Msa0447120:CDS 45.0%
AACTTCAAGAAGGACAGGGA+TGG + chr3_3:24418649-24418668 Msa0447120:CDS 45.0%
ACAGGGATGGTTACTGGAAA+TGG + chr3_3:24418662-24418681 Msa0447120:CDS 45.0%
! GGTTACTGGAAATGGTTTGC+CGG + chr3_3:24418670-24418689 Msa0447120:CDS 45.0%
! GAAATGGTTTGCCGGTAACT+TGG + chr3_3:24418678-24418697 Msa0447120:CDS 45.0%
ATCATTGGCAAGACGAGTAC+GGG - chr3_3:24418761-24418780 None:intergenic 45.0%
! TCTAAGGCAGCTAAGAAAGG+TGG + chr3_3:24418781-24418800 Msa0447120:CDS 45.0%
CAGCTAAGAAAGGTGGAGAA+AGG + chr3_3:24418788-24418807 Msa0447120:CDS 45.0%
CTTGCTACTGATGGAATTGC+TGG + chr3_3:24418847-24418866 Msa0447120:CDS 45.0%
CACAGGAGATGTTGAAACCA+CGG - chr3_3:24418879-24418898 None:intergenic 45.0%
GGTTTCAACATCTCCTGTGT+TGG + chr3_3:24418880-24418899 Msa0447120:CDS 45.0%
GTTTACCGTGGTCTGTACTT+CGG + chr3_3:24418910-24418929 Msa0447120:CDS 45.0%
ACAATCCGAAGTACAGACCA+CGG - chr3_3:24418918-24418937 None:intergenic 45.0%
!! CAGTGAGAAGAACTGGTTTG+AGG - chr3_3:24418948-24418967 None:intergenic 45.0%
CTCAAACCAGTTCTTCTCAC+TGG + chr3_3:24418946-24418965 Msa0447120:CDS 45.0%
CTTGGATGGCTCATTACCAA+TGG + chr3_3:24419141-24419160 Msa0447120:CDS 45.0%
CATACCCCATTGACACTGTT+AGG + chr3_3:24419178-24419197 Msa0447120:CDS 45.0%
CTTGTACTTGACAGCTTCTC+CGG - chr3_3:24419223-24419242 None:intergenic 45.0%
ACTTAGCACCCTCATTCTTG+AGG - chr3_3:24419272-24419291 None:intergenic 45.0%
GGGTGCTAAGTCATTGTTCA+AGG + chr3_3:24419281-24419300 Msa0447120:CDS 45.0%
AAGTCATTGTTCAAGGGAGC+TGG + chr3_3:24419288-24419307 Msa0447120:CDS 45.0%
! TTCTGGTTACGACAAGTTGC+AGG + chr3_3:24419350-24419369 Msa0447120:CDS 45.0%
TTCGGCAAGAAATACGGATC+TGG + chr3_3:24419381-24419400 Msa0447120:CDS 45.0%
AGCACAGGTTAAGGAGATGA+AGG - chr3_3:24420211-24420230 None:intergenic 45.0%
AGAGAGAGAGATGAGTGTGG+AGG - chr3_3:24416811-24416830 None:intergenic 50.0%
GAGAGAGAGAGAGATGAGTG+TGG - chr3_3:24416814-24416833 None:intergenic 50.0%
CAGATGAAAGACCCGATCGA+CGG - chr3_3:24418075-24418094 None:intergenic 50.0%
!! ATCTTTGGGACATGGCAGGA+TGG - chr3_3:24418126-24418145 None:intergenic 50.0%
!! GAGAATCTTTGGGACATGGC+AGG - chr3_3:24418130-24418149 None:intergenic 50.0%
CGCTGCATTGCAGAATCCAA+TGG + chr3_3:24418168-24418187 Msa0447120:CDS 50.0%
TCAACAGTTGCCCTGCAAGT+GGG - chr3_3:24418196-24418215 None:intergenic 50.0%
ATCAACAGTTGCCCTGCAAG+TGG - chr3_3:24418197-24418216 None:intergenic 50.0%
! AATGGGAGCAGCAGCAGTTT+TGG - chr3_3:24418326-24418345 None:intergenic 50.0%
TAGGCTATCTGAGCCCTACA+AGG + chr3_3:24418396-24418415 Msa0447120:CDS 50.0%
AGGCTATCTGAGCCCTACAA+GGG + chr3_3:24418397-24418416 Msa0447120:CDS 50.0%
! TCTGAGCCCTACAAGGGTAT+TGG + chr3_3:24418403-24418422 Msa0447120:CDS 50.0%
! GGTATTGTTGCTCTCTGGAG+AGG + chr3_3:24418457-24418476 Msa0447120:CDS 50.0%
AGAAGGACAGGGATGGTTAC+TGG + chr3_3:24418656-24418675 Msa0447120:CDS 50.0%
TTTGCCGGTAACTTGGCATC+CGG + chr3_3:24418685-24418704 Msa0447120:CDS 50.0%
GGTGGAGAAAGGCAGTTCAA+TGG + chr3_3:24418799-24418818 Msa0447120:CDS 50.0%
! TCGCCAGTTTTGCACTTGGA+TGG + chr3_3:24419127-24419146 Msa0447120:CDS 50.0%
GAGCCATCCAAGTGCAAAAC+TGG - chr3_3:24419133-24419152 None:intergenic 50.0%
TGGCTCATTACCAATGGTGC+AGG + chr3_3:24419147-24419166 Msa0447120:CDS 50.0%
TGATGCTAGACCTGCACCAT+TGG - chr3_3:24419160-24419179 None:intergenic 50.0%
GGCAAGAAATACGGATCTGG+TGG + chr3_3:24419384-24419403 Msa0447120:CDS 50.0%
TCAATCAACGAAGACAGGCC+AGG - chr3_3:24420293-24420312 None:intergenic 50.0%
ATGAAAGACCCGATCGACGG+TGG - chr3_3:24418072-24418091 None:intergenic 55.0%
CGTCGATCGGGTCTTTCATC+TGG + chr3_3:24418073-24418092 Msa0447120:CDS 55.0%
! TTTGGGACATGGCAGGATGG+CGG - chr3_3:24418123-24418142 None:intergenic 55.0%
AACAGTTGCCCTGCAAGTGG+GGG - chr3_3:24418194-24418213 None:intergenic 55.0%
CAACAGTTGCCCTGCAAGTG+GGG - chr3_3:24418195-24418214 None:intergenic 55.0%
ACCTCCGGATGCCAAGTTAC+CGG - chr3_3:24418692-24418711 None:intergenic 55.0%
GGAATTGCTGGGCTTTACCG+TGG + chr3_3:24418859-24418878 Msa0447120:CDS 55.0%
AAGCACACCAGCACCAGCAA+CGG - chr3_3:24419331-24419350 None:intergenic 55.0%
ATCCAATGGCCCCCACTTGC+AGG + chr3_3:24418182-24418201 Msa0447120:CDS 60.0%
TCCAATGGCCCCCACTTGCA+GGG + chr3_3:24418183-24418202 Msa0447120:CDS 60.0%
! AACATCCTCCGTGCCGTTGC+TGG + chr3_3:24419315-24419334 Msa0447120:CDS 60.0%
!! GCTGGTGCTGGTGTGCTTTC+TGG + chr3_3:24419333-24419352 Msa0447120:CDS 60.0%
TGGCCAGCTCCACCGTCGAT+CGG + chr3_3:24418060-24418079 Msa0447120:CDS 65.0%
! GGACATGGCAGGATGGCGGT+AGG - chr3_3:24418119-24418138 None:intergenic 65.0%
GCTGGAGCAGCGACGAAGAC+AGG - chr3_3:24418247-24418266 None:intergenic 65.0%
GCCGGTAACTTGGCATCCGG+AGG + chr3_3:24418688-24418707 Msa0447120:CDS 65.0%
!! TTGGCATCCGGAGGTGCTGC+TGG + chr3_3:24418697-24418716 Msa0447120:CDS 65.0%
! TGAGGCACCAGCAGCACCTC+CGG - chr3_3:24418707-24418726 None:intergenic 65.0%
CACCAGCACCAGCAACGGCA+CGG - chr3_3:24419326-24419345 None:intergenic 65.0%
GGCCAGCTCCACCGTCGATC+GGG + chr3_3:24418061-24418080 Msa0447120:CDS 70.0%
GACCCGATCGACGGTGGAGC+TGG - chr3_3:24418066-24418085 None:intergenic 70.0%
GCCCTGCAAGTGGGGGCCAT+TGG - chr3_3:24418187-24418206 None:intergenic 70.0%
CAGCACCAGCAACGGCACGG+AGG - chr3_3:24419323-24419342 None:intergenic 70.0%
! CTCCGTGCCGTTGCTGGTGC+TGG + chr3_3:24419321-24419340 Msa0447120:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 24416793 24420391 24416793 ID=Msa0447120;Name=Msa0447120
chr3_3 mRNA 24416793 24420391 24416793 ID=Msa0447120-mRNA-1;Parent=Msa0447120;Name=Msa0447120-mRNA-1;_AED=0.05;_eAED=0.05;_QI=98|1|1|1|0.66|0.5|4|978|389
chr3_3 exon 24416793 24416880 24416793 ID=Msa0447120-mRNA-1:exon:4848;Parent=Msa0447120-mRNA-1
chr3_3 exon 24418006 24418516 24418006 ID=Msa0447120-mRNA-1:exon:4849;Parent=Msa0447120-mRNA-1
chr3_3 exon 24418607 24418978 24418607 ID=Msa0447120-mRNA-1:exon:4850;Parent=Msa0447120-mRNA-1
chr3_3 exon 24419117 24420391 24419117 ID=Msa0447120-mRNA-1:exon:4851;Parent=Msa0447120-mRNA-1
chr3_3 five_prime_UTR 24416793 24416880 24416793 ID=Msa0447120-mRNA-1:five_prime_utr;Parent=Msa0447120-mRNA-1
chr3_3 five_prime_UTR 24418006 24418015 24418006 ID=Msa0447120-mRNA-1:five_prime_utr;Parent=Msa0447120-mRNA-1
chr3_3 CDS 24418016 24418516 24418016 ID=Msa0447120-mRNA-1:cds;Parent=Msa0447120-mRNA-1
chr3_3 CDS 24418607 24418978 24418607 ID=Msa0447120-mRNA-1:cds;Parent=Msa0447120-mRNA-1
chr3_3 CDS 24419117 24419413 24419117 ID=Msa0447120-mRNA-1:cds;Parent=Msa0447120-mRNA-1
chr3_3 three_prime_UTR 24419414 24420391 24419414 ID=Msa0447120-mRNA-1:three_prime_utr;Parent=Msa0447120-mRNA-1
Gene Sequence

>Msa0447120

ATGGTTGATCAGGTTAAGCAACCAACAATCATGGAGAAGGTCACTGGCCAGCTCCACCGTCGATCGGGTCTTTCATCTGGATTTAGGGGTTATGAAGGAACCTACCGCCATCCTGCCATGTCCCAAAGATTCTCCTATGGGAACTACTCAAACGCTGCATTGCAGAATCCAATGGCCCCCACTTGCAGGGCAACTGTTGATTTGTCAGCTGTTGCAACAACTGCTTCTCCTGTCTTCGTCGCTGCTCCAGCAGAAAAGAGCCATTTCGTTATTGACTTTCTCATGGGTGGTGTTTCAGCTGCCGTTTCCAAAACTGCTGCTGCTCCCATTGAACGTATTAAGCTTTTGATCCAAAACCAGGATGAGATGATCAAGTCTGGTAGGCTATCTGAGCCCTACAAGGGTATTGGTGATTGTTTTAAAAGAACAATGGCCGATGAGGGTATTGTTGCTCTCTGGAGAGGAAACACTGCCAATGTTATCCGTTATTTCCCTACTCAGGCTTTGAACTTTGCATTCAAAGATTACTTCAAGAGGCTTTTCAACTTCAAGAAGGACAGGGATGGTTACTGGAAATGGTTTGCCGGTAACTTGGCATCCGGAGGTGCTGCTGGTGCCTCATCCCTCTTGTTTGTTTACTCTCTTGATTATGCCCGTACTCGTCTTGCCAATGATTCTAAGGCAGCTAAGAAAGGTGGAGAAAGGCAGTTCAATGGTCTTGTTGATGTCTACAAGAAGACTCTTGCTACTGATGGAATTGCTGGGCTTTACCGTGGTTTCAACATCTCCTGTGTTGGAATCATTGTTTACCGTGGTCTGTACTTCGGATTGTATGATTCCCTCAAACCAGTTCTTCTCACTGGAAAATTGCAGGATAGCTTTTTCGCCAGTTTTGCACTTGGATGGCTCATTACCAATGGTGCAGGTCTAGCATCATACCCCATTGACACTGTTAGGAGAAGAATGATGATGACATCCGGAGAAGCTGTCAAGTACAAGAGTTCCTTTGATGCATTCCAACAAATCCTCAAGAATGAGGGTGCTAAGTCATTGTTCAAGGGAGCTGGTGCTAACATCCTCCGTGCCGTTGCTGGTGCTGGTGTGCTTTCTGGTTACGACAAGTTGCAGGTTCTTGTGTTCGGCAAGAAATACGGATCTGGTGGTGCTTAA
Protein sequence

>Msa0447120

MVDQVKQPTIMEKVTGQLHRRSGLSSGFRGYEGTYRHPAMSQRFSYGNYSNAALQNPMAPTCRATVDLSAVATTASPVFVAAPAEKSHFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKGIGDCFKRTMADEGIVALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLASGGAAGASSLLFVYSLDYARTRLANDSKAAKKGGERQFNGLVDVYKKTLATDGIAGLYRGFNISCVGIIVYRGLYFGLYDSLKPVLLTGKLQDSFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQILKNEGAKSLFKGAGANILRAVAGAGVLSGYDKLQVLVFGKKYGSGGA*