AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0494620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0494620 MtrunA17_Chr3g0088301 98.458 389 6 0 1 389 1 389 0.0 786
Msa0494620 MtrunA17_Chr8g0352031 81.538 390 46 4 1 389 1 365 0.0 607
Msa0494620 MtrunA17_Chr4g0040341 74.615 390 77 3 1 388 1 370 0.0 543
Msa0494620 MtrunA17_Chr2g0328891 74.444 360 83 4 35 389 7 362 4.18e-178 499
Msa0494620 MtrunA17_Chr8g0393031 59.649 285 115 0 92 376 17 301 2.98e-128 371
Msa0494620 MtrunA17_Chr3g0135801 39.189 296 169 6 87 376 28 318 2.15e-67 216
Msa0494620 MtrunA17_Chr3g0135861 31.715 309 177 9 89 376 31 326 9.81e-36 134
Msa0494620 MtrunA17_Chr6g0488081 28.763 299 192 6 89 376 15 303 6.03e-33 125
Msa0494620 MtrunA17_Chr4g0006851 30.029 343 207 10 40 379 46 358 1.13e-28 115
Msa0494620 MtrunA17_Chr5g0396801 29.022 317 172 10 81 363 24 321 2.05e-28 114
Msa0494620 MtrunA17_Chr8g0347451 29.195 298 174 9 92 363 50 336 1.44e-27 112
Msa0494620 MtrunA17_Chr8g0347451 26.108 203 131 5 96 285 157 353 5.82e-11 63.5
Msa0494620 MtrunA17_Chr7g0240341 28.904 301 195 8 90 379 22 314 4.88e-27 109
Msa0494620 MtrunA17_Chr1g0149111 30.314 287 162 11 93 368 139 398 1.29e-26 110
Msa0494620 MtrunA17_Chr1g0149111 27.143 210 132 6 81 286 219 411 3.34e-14 73.6
Msa0494620 MtrunA17_Chr3g0137941 28.881 277 173 8 96 368 125 381 5.65e-26 108
Msa0494620 MtrunA17_Chr3g0137941 28.095 210 130 6 81 286 202 394 1.21e-15 77.8
Msa0494620 MtrunA17_Chr8g0385571 29.617 287 168 9 93 368 227 490 1.23e-25 108
Msa0494620 MtrunA17_Chr7g0252401 30.345 290 159 8 93 368 207 467 2.91e-25 107
Msa0494620 MtrunA17_Chr7g0242311 29.682 283 168 8 104 379 98 356 4.39e-25 105
Msa0494620 MtrunA17_Chr4g0067231 26.444 329 195 8 83 375 4 321 1.24e-24 103
Msa0494620 MtrunA17_Chr4g0063561 27.465 284 174 7 93 368 123 382 6.17e-24 102
Msa0494620 MtrunA17_Chr4g0063561 27.751 209 127 6 83 286 206 395 2.02e-14 74.3
Msa0494620 MtrunA17_Chr5g0436041 26.144 306 209 7 83 379 24 321 7.81e-24 100
Msa0494620 MtrunA17_Chr3g0131501 28.383 303 180 11 77 368 192 468 2.02e-23 101
Msa0494620 MtrunA17_Chr2g0296061 27.673 318 185 9 90 373 12 318 5.42e-23 98.6
Msa0494620 MtrunA17_Chr3g0115801 27.946 297 166 12 96 368 119 391 8.04e-18 84.3
Msa0494620 MtrunA17_Chr4g0000261 24.000 300 186 7 90 368 63 341 2.34e-17 82.8
Msa0494620 MtrunA17_Chr1g0196921 22.180 266 195 4 93 354 14 271 4.70e-17 81.3
Msa0494620 MtrunA17_Chr1g0196921 28.571 168 112 4 94 260 112 272 4.14e-13 69.3
Msa0494620 MtrunA17_Chr2g0331771 25.874 286 195 6 92 373 155 427 2.69e-16 80.1
Msa0494620 MtrunA17_Chr3g0133111 26.174 298 179 10 93 361 6 291 3.33e-16 79.0
Msa0494620 MtrunA17_Chr4g0004571 24.342 304 201 8 77 373 5 286 4.20e-16 78.2
Msa0494620 MtrunA17_Chr5g0411411 26.502 283 187 9 92 363 8 280 7.91e-16 77.4
Msa0494620 MtrunA17_Chr8g0366791 26.316 285 185 9 92 363 8 280 1.82e-15 76.3
Msa0494620 MtrunA17_Chr6g0488341 26.639 244 150 8 141 376 6 228 9.75e-15 73.2
Msa0494620 MtrunA17_Chr7g0241521 25.000 272 187 7 89 354 13 273 1.59e-14 73.6
Msa0494620 MtrunA17_Chr6g0463041 26.244 221 127 5 191 376 18 237 5.96e-14 71.2
Msa0494620 MtrunA17_Chr4g0008061 25.461 271 181 9 99 361 21 278 1.13e-13 71.2
Msa0494620 MtrunA17_Chr8g0382201 23.649 296 180 10 96 368 198 470 5.91e-13 70.1
Msa0494620 MtrunA17_Chr4g0028531 24.742 194 130 4 94 281 116 299 2.57e-11 64.3
Msa0494620 MtrunA17_Chr5g0412401 21.070 299 202 6 93 361 6 300 4.44e-11 63.5
Msa0494620 MtrunA17_Chr7g0269701 22.887 284 194 9 96 368 25 294 5.94e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0494620 AT5G13490.1 81.170 393 62 5 1 389 1 385 0.0 607
Msa0494620 AT5G13490.2 81.170 393 62 5 1 389 1 385 0.0 607
Msa0494620 AT3G08580.2 82.188 393 54 6 1 389 1 381 0.0 591
Msa0494620 AT3G08580.1 82.188 393 54 6 1 389 1 381 0.0 591
Msa0494620 AT4G28390.1 74.936 391 81 4 2 388 1 378 0.0 548
Msa0494620 AT4G28390.2 74.936 391 81 4 2 388 1 378 0.0 548
Msa0494620 AT5G17400.1 58.804 301 124 0 80 380 1 301 6.59e-130 375
Msa0494620 AT5G56450.1 38.835 309 170 7 80 376 21 322 5.53e-66 213
Msa0494620 AT3G55640.1 28.037 321 213 7 58 368 1 313 2.58e-33 127
Msa0494620 AT3G55640.4 28.037 321 213 7 58 368 1 313 2.58e-33 127
Msa0494620 AT2G37890.1 30.640 297 189 7 92 379 44 332 8.25e-33 126
Msa0494620 AT1G14560.1 30.249 281 170 6 104 368 38 308 1.17e-32 125
Msa0494620 AT1G14560.2 30.545 275 165 6 104 362 38 302 6.79e-32 122
Msa0494620 AT4G26180.1 31.270 307 177 9 89 377 17 307 9.91e-32 123
Msa0494620 AT4G26180.2 31.959 291 165 8 89 362 17 291 9.64e-31 119
Msa0494620 AT3G53940.1 29.831 295 187 8 85 368 63 348 3.24e-29 117
Msa0494620 AT3G53940.1 24.365 197 137 3 91 287 178 362 1.12e-11 66.2
Msa0494620 AT3G51870.1 32.624 282 161 9 104 379 102 360 1.44e-28 115
Msa0494620 AT4G01100.1 27.597 308 168 9 96 368 45 332 1.74e-26 108
Msa0494620 AT5G01500.1 30.824 279 170 9 104 379 130 388 2.09e-26 109
Msa0494620 AT4G32400.1 29.893 281 171 9 93 368 114 373 1.34e-25 107
Msa0494620 AT4G01100.2 27.796 313 175 9 96 368 45 346 6.78e-25 104
Msa0494620 AT4G01100.3 26.821 302 166 9 102 368 1 282 2.28e-24 102
Msa0494620 AT5G51050.1 29.825 285 167 7 93 368 223 483 9.83e-24 103
Msa0494620 AT3G21390.1 26.625 323 198 7 83 373 9 324 2.49e-23 100
Msa0494620 AT5G61810.2 27.703 296 183 8 77 368 52 320 2.74e-23 99.8
Msa0494620 AT3G21390.2 26.625 323 198 7 83 373 31 346 3.32e-23 100
Msa0494620 AT5G61810.1 27.703 296 183 8 77 368 195 463 2.18e-22 99.0
Msa0494620 AT3G55640.2 27.481 262 171 6 58 312 1 250 2.66e-22 95.5
Msa0494620 AT3G55640.3 27.481 262 171 6 58 312 1 250 2.66e-22 95.5
Msa0494620 AT1G14560.3 31.383 188 112 4 197 368 24 210 7.81e-22 93.6
Msa0494620 AT3G20240.1 24.917 301 190 6 86 368 45 327 8.12e-22 95.9
Msa0494620 AT5G48970.1 27.160 324 196 10 83 373 12 328 1.52e-21 95.1
Msa0494620 AT5G07320.1 27.778 270 169 7 102 368 218 464 5.68e-21 94.7
Msa0494620 AT3G53940.2 29.167 216 137 5 85 293 63 269 8.27e-19 86.3
Msa0494620 AT5G64970.1 27.458 295 170 11 96 368 138 410 3.95e-15 77.0
Msa0494620 AT5G48970.2 26.641 259 157 8 141 373 6 257 5.43e-15 75.1
Msa0494620 AT1G14140.1 23.507 268 190 6 93 354 16 274 1.36e-14 74.3
Msa0494620 AT1G14140.1 28.061 196 127 5 198 383 18 209 1.20e-11 65.5
Msa0494620 AT5G64970.2 26.780 295 172 11 96 368 14 286 1.63e-14 73.9
Msa0494620 AT5G64970.3 27.778 288 164 11 96 361 138 403 3.60e-14 73.9
Msa0494620 AT5G46800.2 26.573 286 186 8 92 363 7 282 6.68e-14 72.4
Msa0494620 AT5G46800.3 26.573 286 186 8 92 363 7 282 6.68e-14 72.4
Msa0494620 AT5G46800.1 26.573 286 186 8 92 363 7 282 6.68e-14 72.4
Msa0494620 AT5G58970.1 24.742 291 190 8 83 361 6 279 8.23e-14 72.0
Msa0494620 AT2G33820.1 23.404 282 201 5 89 362 14 288 3.61e-13 70.1
Msa0494620 AT5G66380.1 27.317 205 125 6 191 383 5 197 1.24e-12 68.6
Msa0494620 AT3G54110.1 23.134 268 189 8 93 354 15 271 1.39e-12 68.6
Msa0494620 AT1G78180.1 24.415 299 174 8 96 368 131 403 8.83e-12 66.6
Msa0494620 AT1G79900.1 25.726 241 156 6 140 373 53 277 9.07e-12 65.9
Msa0494620 AT2G33820.3 22.759 290 201 6 89 362 14 296 8.43e-11 63.2

Find 165 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGATATGTATGCTATTT+TGG 0.104027 3_4:+22892236 Msa0494620:three_prime_UTR
AATTGAAAACATGATTTATT+TGG 0.123351 3_4:+22891789 Msa0494620:three_prime_UTR
GCTGGTGCTGGTGTGCTTTC+TGG 0.149238 3_4:+22891551 Msa0494620:CDS
TGATCATCTCATCCTGGTTT+TGG 0.179358 3_4:-22890584 None:intergenic
CGGGTCTTTCATCTGGATTT+AGG 0.206368 3_4:+22890298 Msa0494620:CDS
AACCAAAATTTAAAAGAGTT+TGG 0.219808 3_4:-22891756 None:intergenic
GTTTATTGATTGATTGTATA+AGG 0.227083 3_4:-22891970 None:intergenic
TATTTCATGATCATCCTTAT+TGG 0.235542 3_4:+22891913 Msa0494620:three_prime_UTR
AATGGGAGCAGCAGCAGTTT+TGG 0.270825 3_4:-22890541 None:intergenic
GGGTCTTTCATCTGGATTTA+GGG 0.271738 3_4:+22890299 Msa0494620:CDS
ACCAAAATTTAAAAGAGTTT+GGG 0.303902 3_4:-22891755 None:intergenic
AGTTCCCATAGGAGAATCTT+TGG 0.306546 3_4:-22890356 None:intergenic
TCTTCTCACTGGAAAATTGC+AGG 0.310516 3_4:+22891175 Msa0494620:CDS
TAATCACACCATTAAGAAAA+AGG 0.319564 3_4:-22892082 None:intergenic
CTTAGCTGCCTTAGAATCAT+TGG 0.323339 3_4:-22890991 None:intergenic
AGTTAGCATGACATTAGTTT+AGG 0.326894 3_4:-22892132 None:intergenic
CTTGCTACTGATGGAATTGC+TGG 0.330650 3_4:+22891065 Msa0494620:CDS
AGAAGGACAGGGATGGTTAC+TGG 0.330831 3_4:+22890874 Msa0494620:CDS
TTCTAATGATTTAGCCATCT+TGG 0.333491 3_4:-22892324 None:intergenic
CCTCATTCTTGAGGATTTGT+TGG 0.335787 3_4:-22891478 None:intergenic
TCTAGTTAAGAAAACCAAAA+AGG 0.338031 3_4:+22892166 Msa0494620:three_prime_UTR
GTATGAAAATTGTCTAAAAT+TGG 0.343683 3_4:+22892386 Msa0494620:three_prime_UTR
GAGCCATCCAAGCGCAAAAC+TGG 0.344763 3_4:-22891348 None:intergenic
AACATCCTCCGAGCCGTTGC+TGG 0.344926 3_4:+22891533 Msa0494620:CDS
CTCCGAGCCGTTGCTGGTGC+TGG 0.348272 3_4:+22891539 Msa0494620:CDS
CGTCGATCGGGTCTTTCATC+TGG 0.364825 3_4:+22890291 Msa0494620:CDS
GAGCAAGATTCTAACCAATA+AGG 0.365498 3_4:-22891927 None:intergenic
TTTCATATGGAAAATGGAAA+TGG 0.368498 3_4:-22892480 None:intergenic
ACAATCACCAATACCCTTGT+AGG 0.369255 3_4:-22890628 None:intergenic
TAAACTAATGTCATGCTAAC+TGG 0.375168 3_4:+22892134 Msa0494620:three_prime_UTR
CGTTCTACAACAAATATTAC+TGG 0.388157 3_4:-22892276 None:intergenic
GTGACCTTCTCCATGATTGT+TGG 0.389407 3_4:-22890255 None:intergenic
CAAAAGCTTAATACGTTCAA+TGG 0.391915 3_4:-22890559 None:intergenic
TCGTCTTGCCAATGATTCTA+AGG 0.397580 3_4:+22890983 Msa0494620:CDS
ACAGGGATGGTTACTGGAAA+TGG 0.399761 3_4:+22890880 Msa0494620:CDS
TGTGTAGTCAAAGTATAAAA+TGG 0.401063 3_4:-22892209 None:intergenic
TTGCACATTAATTTATTTGA+GGG 0.408466 3_4:+22892349 Msa0494620:three_prime_UTR
CGTAGCCATACACGTTTCAT+AGG 0.408982 3_4:-22892594 None:intergenic
CATGATTTATTTGGAGTGGC+AGG 0.411321 3_4:+22891798 Msa0494620:three_prime_UTR
TTGGCATCCGGAGGTGCTGC+TGG 0.411978 3_4:+22890915 Msa0494620:CDS
TTCTGGTTACGACAAGTTGC+AGG 0.414142 3_4:+22891568 Msa0494620:CDS
GAAAGCAACCATGGTTGATC+AGG 0.420030 3_4:+22890224 Msa0494620:exon
CTTCTCCTAACAGTGTCAAT+GGG 0.420342 3_4:-22891401 None:intergenic
TTAGTCACAAGCACAGATTA+AGG 0.421193 3_4:-22892433 None:intergenic
CCAAAATAGCATACATATCA+TGG 0.423680 3_4:-22892236 None:intergenic
TGAGGCACCAGCAGCACCTC+CGG 0.430152 3_4:-22890922 None:intergenic
TTTCGTTATTGACTTTCTCA+TGG 0.438686 3_4:+22890497 Msa0494620:CDS
CTTGTGTTCGGCAAGAAATA+CGG 0.440747 3_4:+22891593 Msa0494620:CDS
GACCCGATCGACGGTGGAGC+TGG 0.442042 3_4:-22890281 None:intergenic
AAGTTGCAGGTTCTTGTGTT+CGG 0.444616 3_4:+22891581 Msa0494620:CDS
CTTGTACTTGACAGCTTCTC+CGG 0.448393 3_4:-22891438 None:intergenic
GGCCAGCTCCACCGTCGATC+GGG 0.448817 3_4:+22890279 Msa0494620:CDS
AGTGAGAAGAACTGGTTTGA+GGG 0.449368 3_4:-22891162 None:intergenic
CAGTGAGAAGAACTGGTTTG+AGG 0.452118 3_4:-22891163 None:intergenic
GGTTACTGGAAATGGTTTGC+CGG 0.452634 3_4:+22890888 Msa0494620:CDS
TCTTCTCCTAACAGTGTCAA+TGG 0.452687 3_4:-22891402 None:intergenic
GAAATGGTTTGCCGGTAACT+TGG 0.453917 3_4:+22890896 Msa0494620:CDS
ACCTCCGGATGCCAAGTTAC+CGG 0.453929 3_4:-22890907 None:intergenic
AAATACCAAATGAATAAAAC+AGG 0.456337 3_4:-22892108 None:intergenic
TGAGAAAGTCAATAACGAAA+TGG 0.456385 3_4:-22890494 None:intergenic
CTCAAACCAGTTCTTCTCAC+TGG 0.459621 3_4:+22891164 Msa0494620:CDS
ATTGCACATTAATTTATTTG+AGG 0.461124 3_4:+22892348 Msa0494620:three_prime_UTR
GTTCCCATAGGAGAATCTTT+GGG 0.471265 3_4:-22890355 None:intergenic
GTAGAACGATGAACAATTAC+TGG 0.472700 3_4:+22892291 Msa0494620:three_prime_UTR
CATACCCCATTGACACTGTT+AGG 0.473018 3_4:+22891396 Msa0494620:CDS
TGGCCAGCTCCACCGTCGAT+CGG 0.474097 3_4:+22890278 Msa0494620:CDS
GCTGGAGCAGCGACGAAGAC+AGG 0.475793 3_4:-22890462 None:intergenic
TCTGGATTTAGGGGTTATGA+AGG 0.477435 3_4:+22890309 Msa0494620:CDS
ATCATTGGCAAGACGAGTAC+GGG 0.477887 3_4:-22890976 None:intergenic
AGAAAATGGCTAACCTGAGT+AGG 0.478242 3_4:-22890726 None:intergenic
ATGAGGGTATTGTTGCTCTC+TGG 0.479479 3_4:+22890670 Msa0494620:CDS
AACACCTACTATCATCATAC+TGG 0.479860 3_4:-22891997 None:intergenic
ATCAACAGTTGCCCTGCAAG+TGG 0.483410 3_4:-22890412 None:intergenic
TCAATCAACGAAGACAGGCC+AGG 0.487277 3_4:-22892506 None:intergenic
AAAAGCTTAATACGTTCAAT+GGG 0.489695 3_4:-22890558 None:intergenic
TCTGAGCCCTACAAGGGTAT+TGG 0.490496 3_4:+22890621 Msa0494620:CDS
AATACCAGTATGATGATAGT+AGG 0.491772 3_4:+22891993 Msa0494620:three_prime_UTR
ATGTCCCAAAGATTCTCCTA+TGG 0.492338 3_4:+22890351 Msa0494620:CDS
GGTTTCAACATCTCCTGTGT+TGG 0.492793 3_4:+22891098 Msa0494620:CDS
TTCGTTATTGACTTTCTCAT+GGG 0.493555 3_4:+22890498 Msa0494620:CDS
CCAACAAATCCTCAAGAATG+AGG 0.494163 3_4:+22891478 Msa0494620:CDS
CAGGATGAGATGATCAAGTC+TGG 0.496357 3_4:+22890591 Msa0494620:CDS
GGGTGCTAAGTCATTGTTCA+AGG 0.502290 3_4:+22891499 Msa0494620:CDS
AATCATTGGCAAGACGAGTA+CGG 0.503375 3_4:-22890977 None:intergenic
CCATACACGTTTCATAGGCC+AGG 0.503398 3_4:-22892589 None:intergenic
AAGAAGACTCTTGCTACTGA+TGG 0.508472 3_4:+22891056 Msa0494620:CDS
CACCAGCACCAGCAACGGCT+CGG 0.509084 3_4:-22891541 None:intergenic
GGACATGGCAGGATGGTGGT+AGG 0.514010 3_4:-22890334 None:intergenic
ACAATCATGGAGAAGGTCAC+TGG 0.514525 3_4:+22890258 Msa0494620:CDS
AAGCACACCAGCACCAGCAA+CGG 0.516455 3_4:-22891546 None:intergenic
GTTTAATAAATTTACTCGGT+GGG 0.517965 3_4:+22891725 Msa0494620:three_prime_UTR
GTTTACCGTGGTCTGTACTT+CGG 0.519266 3_4:+22891128 Msa0494620:CDS
TTCGGCAAGAAATACGGATC+TGG 0.519522 3_4:+22891599 Msa0494620:CDS
CAGACTTGATCATCTCATCC+TGG 0.520517 3_4:-22890590 None:intergenic
AAGTCATTGTTCAAGGGAGC+TGG 0.521554 3_4:+22891506 Msa0494620:CDS
TTTGCCGGTAACTTGGCATC+CGG 0.521620 3_4:+22890903 Msa0494620:CDS
AGCACAGATTAAGGAGATGA+AGG 0.522430 3_4:-22892424 None:intergenic
GGTTAAGCAACCAACAATCA+TGG 0.524656 3_4:+22890245 Msa0494620:CDS
TGATGCTAGACCTGCACCAT+TGG 0.527310 3_4:-22891375 None:intergenic
AGAAGAATGATGATGACATC+CGG 0.527583 3_4:+22891419 Msa0494620:CDS
CTTTGTCAATCAACGAAGAC+AGG 0.528211 3_4:-22892511 None:intergenic
CAACAGTTGCCCTGCAAGTG+GGG 0.528651 3_4:-22890410 None:intergenic
TGGCTCATTACCAATGGTGC+AGG 0.529441 3_4:+22891365 Msa0494620:CDS
TATCCGTTATTTCCCTACTC+AGG 0.534487 3_4:+22890713 Msa0494620:CDS
TAACCTGAGTAGGGAAATAA+CGG 0.535034 3_4:-22890716 None:intergenic
CATTCAAAGATTACTTCAAG+AGG 0.536246 3_4:+22890838 Msa0494620:CDS
TAGGCTATCTGAGCCCTACA+AGG 0.536940 3_4:+22890614 Msa0494620:CDS
GATTCTAAGGCAGCTAAGAA+AGG 0.539062 3_4:+22890996 Msa0494620:CDS
CAGCTAAGAAAGGTGGAGAA+AGG 0.539801 3_4:+22891006 Msa0494620:CDS
CAGGATAAGTAACTGTCACT+TGG 0.543016 3_4:+22891817 Msa0494620:three_prime_UTR
GAAAATGGCTAACCTGAGTA+GGG 0.543044 3_4:-22890725 None:intergenic
GAGAATCTTTGGGACATGGC+AGG 0.547914 3_4:-22890345 None:intergenic
GTTATTGACTTTCTCATGGG+TGG 0.552168 3_4:+22890501 Msa0494620:CDS
TTGCTACTGATGGAATTGCT+GGG 0.552525 3_4:+22891066 Msa0494620:CDS
TGATCATGAAATAAAACTCT+AGG 0.552839 3_4:-22891903 None:intergenic
ACTTAGCACCCTCATTCTTG+AGG 0.554699 3_4:-22891487 None:intergenic
AGAGAGAGAAATGTGTGTGG+AGG 0.555231 3_4:-22889093 None:intergenic
GCCGGTAACTTGGCATCCGG+AGG 0.555811 3_4:+22890906 Msa0494620:CDS
TCAACAGTTGCCCTGCAAGT+GGG 0.556580 3_4:-22890411 None:intergenic
AAAACATGATTTATTTGGAG+TGG 0.558044 3_4:+22891794 Msa0494620:three_prime_UTR
AAAAGAACAATGGCCGATGA+GGG 0.560661 3_4:+22890654 Msa0494620:CDS
TGTCCCAAAGATTCTCCTAT+GGG 0.564873 3_4:+22890352 Msa0494620:CDS
GATTTGTTGGAATGCATCAA+AGG 0.565548 3_4:-22891465 None:intergenic
GGTGGAGAAAGGCAGTTCAA+TGG 0.572074 3_4:+22891017 Msa0494620:CDS
AAGAGAGAGAGAAATGTGTG+TGG 0.572476 3_4:-22889096 None:intergenic
TAAAAGAACAATGGCCGATG+AGG 0.576304 3_4:+22890653 Msa0494620:CDS
CTTGGATGGCTCATTACCAA+TGG 0.580145 3_4:+22891359 Msa0494620:CDS
GGTCTTTCATCTGGATTTAG+GGG 0.580823 3_4:+22890300 Msa0494620:CDS
CAATCACCAATACCCTTGTA+GGG 0.581476 3_4:-22890627 None:intergenic
GAAGTGTTACCTTTCACGAA+GGG 0.581786 3_4:-22889143 None:intergenic
AACAGTTGCCCTGCAAGTGG+GGG 0.584275 3_4:-22890409 None:intergenic
GAGAGCAACAATACCCTCAT+CGG 0.586821 3_4:-22890667 None:intergenic
TGTTTAATAAATTTACTCGG+TGG 0.590627 3_4:+22891724 Msa0494620:three_prime_UTR
TGAAGTGTTACCTTTCACGA+AGG 0.592018 3_4:-22889144 None:intergenic
ATCTTTGGGACATGGCAGGA+TGG 0.596363 3_4:-22890341 None:intergenic
ATGAGATGATCAAGTCTGGT+AGG 0.599652 3_4:+22890595 Msa0494620:CDS
CAAGAGAGTAAACAAACAAG+AGG 0.602030 3_4:-22890947 None:intergenic
CAGATGAAAGACCCGATCGA+CGG 0.603535 3_4:-22890290 None:intergenic
AACTTCAAGAAGGACAGGGA+TGG 0.606997 3_4:+22890867 Msa0494620:CDS
CGCTGCATTGCAGAATCCAA+TGG 0.607456 3_4:+22890386 Msa0494620:CDS
CAACAAATCCTCAAGAATGA+GGG 0.607876 3_4:+22891479 Msa0494620:CDS
ATGAAAGACCCGATCGACGG+TGG 0.608954 3_4:-22890287 None:intergenic
GGTATTGTTGCTCTCTGGAG+AGG 0.610023 3_4:+22890675 Msa0494620:CDS
AGTATGATGATAGTAGGTGT+TGG 0.622229 3_4:+22891999 Msa0494620:three_prime_UTR
GCAACCAACAATCATGGAGA+AGG 0.628211 3_4:+22890251 Msa0494620:CDS
GGCAAGAAATACGGATCTGG+TGG 0.628657 3_4:+22891602 Msa0494620:CDS
TCTAAGGCAGCTAAGAAAGG+TGG 0.629619 3_4:+22890999 Msa0494620:CDS
GTAAACAAACAAGAGGGATG+AGG 0.631365 3_4:-22890940 None:intergenic
CACAGGAGATGTTGAAACCA+CGG 0.633206 3_4:-22891094 None:intergenic
ATAGGAGAATCTTTGGGACA+TGG 0.635000 3_4:-22890349 None:intergenic
TTTCAACTTCAAGAAGGACA+GGG 0.637221 3_4:+22890863 Msa0494620:CDS
GTAAACAATGATTCCAACAC+AGG 0.637438 3_4:-22891111 None:intergenic
AGGGAAATAACGGATAACAT+TGG 0.640612 3_4:-22890706 None:intergenic
GGTGCTAAGTCATTGTTCAA+GGG 0.642143 3_4:+22891500 Msa0494620:CDS
GCAGCAATTAGTCTAACTCT+CGG 0.646966 3_4:-22891692 None:intergenic
TTTGGGACATGGCAGGATGG+TGG 0.651052 3_4:-22890338 None:intergenic
GGAATTGCTGGGCTTTACCG+TGG 0.654046 3_4:+22891077 Msa0494620:CDS
AAGAGAGTAAACAAACAAGA+GGG 0.655445 3_4:-22890946 None:intergenic
CAGCACCAGCAACGGCTCGG+AGG 0.656828 3_4:-22891538 None:intergenic
AGCGTTTGAGTAGTTCCCAT+AGG 0.663946 3_4:-22890367 None:intergenic
TTGCTTAACCTGATCAACCA+TGG 0.675233 3_4:-22890232 None:intergenic
TTCTCCTAACAGTGTCAATG+GGG 0.682740 3_4:-22891400 None:intergenic
GTTGGAATCATTGTTTACCG+TGG 0.683302 3_4:+22891116 Msa0494620:CDS
AGGCTATCTGAGCCCTACAA+GGG 0.684234 3_4:+22890615 Msa0494620:CDS
ACAATCCGAAGTACAGACCA+CGG 0.718426 3_4:-22891133 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTTGAAGATTATAT+TGG + chr3_4:22890120-22890139 Msa0494620:intron 10.0%
!! ATGATAATATAAATAAACTA+AGG - chr3_4:22890163-22890182 None:intergenic 10.0%
!!! TTTTGTTTAATAAATTTACT+CGG + chr3_4:22891721-22891740 Msa0494620:three_prime_UTR 10.0%
!!! TAATATAAAAATTTCCTTTT+TGG - chr3_4:22892183-22892202 None:intergenic 10.0%
!! TAAAATTGAAAACTAGAAAA+TGG - chr3_4:22890743-22890762 None:intergenic 15.0%
!!! TCAAATTTATTGTTTTTAGA+TGG + chr3_4:22891649-22891668 Msa0494620:three_prime_UTR 15.0%
!! AATTGAAAACATGATTTATT+TGG + chr3_4:22891789-22891808 Msa0494620:three_prime_UTR 15.0%
!!! TGTTATATCTTGATTTTATA+TGG + chr3_4:22892042-22892061 Msa0494620:three_prime_UTR 15.0%
!!! GTTATATCTTGATTTTATAT+GGG + chr3_4:22892043-22892062 Msa0494620:three_prime_UTR 15.0%
!!! TTATATCTTGATTTTATATG+GGG + chr3_4:22892044-22892063 Msa0494620:three_prime_UTR 15.0%
!!! TTGAATTTTTATTCGATTTG+AGG + chr3_4:22889283-22889302 Msa0494620:intron 20.0%
!!! GAGGTTTTTTTTTTAATTTG+TGG + chr3_4:22889302-22889321 Msa0494620:intron 20.0%
!!! AATTTTGTGATCTAGATAAT+TGG + chr3_4:22889779-22889798 Msa0494620:intron 20.0%
!! TTTGAAGATTATATTGGATA+TGG + chr3_4:22890126-22890145 Msa0494620:intron 20.0%
!!! TGATTGTTTTAAAAGAACAA+TGG + chr3_4:22890644-22890663 Msa0494620:CDS 20.0%
!! CATTAAATACACAAAACAAT+TGG - chr3_4:22890773-22890792 None:intergenic 20.0%
!! ATGAATATCATTTGTTCAAA+GGG - chr3_4:22891213-22891232 None:intergenic 20.0%
!! AATGAATATCATTTGTTCAA+AGG - chr3_4:22891214-22891233 None:intergenic 20.0%
!! TGCAACGAAAAATAATAATT+TGG - chr3_4:22891241-22891260 None:intergenic 20.0%
!! ACCAAAATTTAAAAGAGTTT+GGG - chr3_4:22891758-22891777 None:intergenic 20.0%
!! AACCAAAATTTAAAAGAGTT+TGG - chr3_4:22891759-22891778 None:intergenic 20.0%
!!! AAACTCTTTTAAATTTTGGT+TGG + chr3_4:22891758-22891777 Msa0494620:three_prime_UTR 20.0%
!!! GTTTATTGATTGATTGTATA+AGG - chr3_4:22891973-22891992 None:intergenic 20.0%
!! AAATACCAAATGAATAAAAC+AGG - chr3_4:22892111-22892130 None:intergenic 20.0%
!! ATTGCACATTAATTTATTTG+AGG + chr3_4:22892348-22892367 Msa0494620:three_prime_UTR 20.0%
!! TTGCACATTAATTTATTTGA+GGG + chr3_4:22892349-22892368 Msa0494620:three_prime_UTR 20.0%
!!! GTATGAAAATTGTCTAAAAT+TGG + chr3_4:22892386-22892405 Msa0494620:three_prime_UTR 20.0%
!!! TACTGTTGATTTGATTCATA+CGG + chr3_4:22889238-22889257 Msa0494620:intron 25.0%
!! ATTCATACGGTTTTTCATTT+TGG + chr3_4:22889251-22889270 Msa0494620:intron 25.0%
! ACAGAATTGTAACTACTAAT+TGG - chr3_4:22889558-22889577 None:intergenic 25.0%
! TTGTCTGTATATAAAACTTG+TGG + chr3_4:22889608-22889627 Msa0494620:intron 25.0%
! AAGATTTGTAAACTGAATTG+TGG - chr3_4:22889680-22889699 None:intergenic 25.0%
! ACAATTCAGTTTACAAATCT+TGG + chr3_4:22889679-22889698 Msa0494620:intron 25.0%
! ACTAAATTATCTTCCAGAAA+AGG - chr3_4:22889746-22889765 None:intergenic 25.0%
!! TAATTGGTTGATTCTTTAGA+TGG + chr3_4:22889795-22889814 Msa0494620:intron 25.0%
! CATTGAATAATTGATGTATC+TGG - chr3_4:22889881-22889900 None:intergenic 25.0%
! AAATTGATCAAAGACTACAT+CGG - chr3_4:22889937-22889956 None:intergenic 25.0%
! TTAATCTGATTCAGAATTAG+AGG + chr3_4:22889990-22890009 Msa0494620:intron 25.0%
!! TATTGGATATGGATTGATTA+TGG + chr3_4:22890137-22890156 Msa0494620:intron 25.0%
! TAATCGACTAATGTCAAATA+AGG - chr3_4:22890194-22890213 None:intergenic 25.0%
!! ATTAGTCGATTACATTTTTC+AGG + chr3_4:22890202-22890221 Msa0494620:intron 25.0%
! AAAAGCTTAATACGTTCAAT+GGG - chr3_4:22890561-22890580 None:intergenic 25.0%
!! TCTGCTATTTAAATTGTTCT+AGG + chr3_4:22890803-22890822 Msa0494620:intron 25.0%
! TGTTTAATAAATTTACTCGG+TGG + chr3_4:22891724-22891743 Msa0494620:three_prime_UTR 25.0%
! GTTTAATAAATTTACTCGGT+GGG + chr3_4:22891725-22891744 Msa0494620:three_prime_UTR 25.0%
!! TCCCAAACTCTTTTAAATTT+TGG + chr3_4:22891754-22891773 Msa0494620:three_prime_UTR 25.0%
! AAAACATGATTTATTTGGAG+TGG + chr3_4:22891794-22891813 Msa0494620:three_prime_UTR 25.0%
! TGATCATGAAATAAAACTCT+AGG - chr3_4:22891906-22891925 None:intergenic 25.0%
! TATTTCATGATCATCCTTAT+TGG + chr3_4:22891913-22891932 Msa0494620:three_prime_UTR 25.0%
! TAATCACACCATTAAGAAAA+AGG - chr3_4:22892085-22892104 None:intergenic 25.0%
!! TTTTCTTAATGGTGTGATTA+TGG + chr3_4:22892085-22892104 Msa0494620:three_prime_UTR 25.0%
!! TGGTTTTCTTAACTAGAAAA+CGG - chr3_4:22892163-22892182 None:intergenic 25.0%
! TCTAGTTAAGAAAACCAAAA+AGG + chr3_4:22892166-22892185 Msa0494620:three_prime_UTR 25.0%
! TGTGTAGTCAAAGTATAAAA+TGG - chr3_4:22892212-22892231 None:intergenic 25.0%
! TTTCATATGGAAAATGGAAA+TGG - chr3_4:22892483-22892502 None:intergenic 25.0%
!! AGGATTTTTCATATGGAAAA+TGG - chr3_4:22892489-22892508 None:intergenic 25.0%
!! ATTTTCCATATGAAAAATCC+TGG + chr3_4:22892488-22892507 Msa0494620:three_prime_UTR 25.0%
! ACAAAGTAAAGTAAAAATCC+TGG + chr3_4:22892571-22892590 Msa0494620:three_prime_UTR 25.0%
!! TGTGATTGGATCTTAGTTTT+TGG + chr3_4:22889448-22889467 Msa0494620:intron 30.0%
AGTAGTTACAATTCTGTACT+TGG + chr3_4:22889561-22889580 Msa0494620:intron 30.0%
!! TGGTTCTGTTAGTATTTTGT+TGG + chr3_4:22889699-22889718 Msa0494620:intron 30.0%
!! TTTGTTGGCTTTAGAATTTG+TGG + chr3_4:22889714-22889733 Msa0494620:intron 30.0%
!! TTTGTGGATTATACCTTTTC+TGG + chr3_4:22889730-22889749 Msa0494620:intron 30.0%
AATTGATCAAAGACTACATC+GGG - chr3_4:22889936-22889955 None:intergenic 30.0%
TGAGAAAGTCAATAACGAAA+TGG - chr3_4:22890497-22890516 None:intergenic 30.0%
!! TTTCGTTATTGACTTTCTCA+TGG + chr3_4:22890497-22890516 Msa0494620:CDS 30.0%
!! TTCGTTATTGACTTTCTCAT+GGG + chr3_4:22890498-22890517 Msa0494620:CDS 30.0%
CAAAAGCTTAATACGTTCAA+TGG - chr3_4:22890562-22890581 None:intergenic 30.0%
CATTCAAAGATTACTTCAAG+AGG + chr3_4:22890838-22890857 Msa0494620:CDS 30.0%
AAGAGAGTAAACAAACAAGA+GGG - chr3_4:22890949-22890968 None:intergenic 30.0%
! TTTTGAACTCTTGACATTAC+AGG + chr3_4:22891313-22891332 Msa0494620:intron 30.0%
AATACCAGTATGATGATAGT+AGG + chr3_4:22891993-22892012 Msa0494620:three_prime_UTR 30.0%
! ATCTTGATTTTATATGGGGA+TGG + chr3_4:22892048-22892067 Msa0494620:three_prime_UTR 30.0%
!! TATGGCCTGTTTTATTCATT+TGG + chr3_4:22892103-22892122 Msa0494620:three_prime_UTR 30.0%
! AGTTAGCATGACATTAGTTT+AGG - chr3_4:22892135-22892154 None:intergenic 30.0%
TAAACTAATGTCATGCTAAC+TGG + chr3_4:22892134-22892153 Msa0494620:three_prime_UTR 30.0%
! GGTTTTCTTAACTAGAAAAC+GGG - chr3_4:22892162-22892181 None:intergenic 30.0%
CCAAAATAGCATACATATCA+TGG - chr3_4:22892239-22892258 None:intergenic 30.0%
!! CCATGATATGTATGCTATTT+TGG + chr3_4:22892236-22892255 Msa0494620:three_prime_UTR 30.0%
CGTTCTACAACAAATATTAC+TGG - chr3_4:22892279-22892298 None:intergenic 30.0%
TTCTAATGATTTAGCCATCT+TGG - chr3_4:22892327-22892346 None:intergenic 30.0%
ACTGTTTGTGTTTGTCTGTT+CGG + chr3_4:22889389-22889408 Msa0494620:intron 35.0%
! TCAGATTGTTTCTCTGTGAT+TGG + chr3_4:22889434-22889453 Msa0494620:intron 35.0%
CGTTGTGTGATTGATGTATA+TGG + chr3_4:22889842-22889861 Msa0494620:intron 35.0%
!!! ATTTTTCAGGAAAGCAACCA+TGG + chr3_4:22890215-22890234 Msa0494620:intron 35.0%
! TAAGCTTTTGATCCAAAACC+AGG + chr3_4:22890572-22890591 Msa0494620:CDS 35.0%
!! AGGGAAATAACGGATAACAT+TGG - chr3_4:22890709-22890728 None:intergenic 35.0%
TAACCTGAGTAGGGAAATAA+CGG - chr3_4:22890719-22890738 None:intergenic 35.0%
! TTTTCAACTTCAAGAAGGAC+AGG + chr3_4:22890862-22890881 Msa0494620:CDS 35.0%
TTTCAACTTCAAGAAGGACA+GGG + chr3_4:22890863-22890882 Msa0494620:CDS 35.0%
CAAGAGAGTAAACAAACAAG+AGG - chr3_4:22890950-22890969 None:intergenic 35.0%
GTAAACAATGATTCCAACAC+AGG - chr3_4:22891114-22891133 None:intergenic 35.0%
! AATTTTCCAGTGAGAAGAAC+TGG - chr3_4:22891173-22891192 None:intergenic 35.0%
! AGATCAAAAGTATGTTGCAG+TGG - chr3_4:22891270-22891289 None:intergenic 35.0%
! AGAAGAATGATGATGACATC+CGG + chr3_4:22891419-22891438 Msa0494620:CDS 35.0%
!! GATTTGTTGGAATGCATCAA+AGG - chr3_4:22891468-22891487 None:intergenic 35.0%
CAACAAATCCTCAAGAATGA+GGG + chr3_4:22891479-22891498 Msa0494620:CDS 35.0%
GAGCAAGATTCTAACCAATA+AGG - chr3_4:22891930-22891949 None:intergenic 35.0%
AACACCTACTATCATCATAC+TGG - chr3_4:22892000-22892019 None:intergenic 35.0%
AGTATGATGATAGTAGGTGT+TGG + chr3_4:22891999-22892018 Msa0494620:three_prime_UTR 35.0%
! GGTTCATGCCTTTTTCTTAA+TGG + chr3_4:22892074-22892093 Msa0494620:three_prime_UTR 35.0%
GTAGAACGATGAACAATTAC+TGG + chr3_4:22892291-22892310 Msa0494620:three_prime_UTR 35.0%
TTAGTCACAAGCACAGATTA+AGG - chr3_4:22892436-22892455 None:intergenic 35.0%
AAGAGAGAGAGAAATGTGTG+TGG - chr3_4:22889099-22889118 None:intergenic 40.0%
! GAAGTGTTACCTTTCACGAA+GGG - chr3_4:22889146-22889165 None:intergenic 40.0%
! TGAAGTGTTACCTTTCACGA+AGG - chr3_4:22889147-22889166 None:intergenic 40.0%
TTTGTCTGTTCGGATCTGAT+TGG + chr3_4:22889399-22889418 Msa0494620:intron 40.0%
TTGCTTAACCTGATCAACCA+TGG - chr3_4:22890235-22890254 None:intergenic 40.0%
GGTTAAGCAACCAACAATCA+TGG + chr3_4:22890245-22890264 Msa0494620:CDS 40.0%
GGGTCTTTCATCTGGATTTA+GGG + chr3_4:22890299-22890318 Msa0494620:CDS 40.0%
GGTCTTTCATCTGGATTTAG+GGG + chr3_4:22890300-22890319 Msa0494620:CDS 40.0%
TCTGGATTTAGGGGTTATGA+AGG + chr3_4:22890309-22890328 Msa0494620:CDS 40.0%
!! ATAGGAGAATCTTTGGGACA+TGG - chr3_4:22890352-22890371 None:intergenic 40.0%
ATGTCCCAAAGATTCTCCTA+TGG + chr3_4:22890351-22890370 Msa0494620:CDS 40.0%
TGTCCCAAAGATTCTCCTAT+GGG + chr3_4:22890352-22890371 Msa0494620:CDS 40.0%
GTTCCCATAGGAGAATCTTT+GGG - chr3_4:22890358-22890377 None:intergenic 40.0%
AGTTCCCATAGGAGAATCTT+TGG - chr3_4:22890359-22890378 None:intergenic 40.0%
!! GTTATTGACTTTCTCATGGG+TGG + chr3_4:22890501-22890520 Msa0494620:CDS 40.0%
! TGATCATCTCATCCTGGTTT+TGG - chr3_4:22890587-22890606 None:intergenic 40.0%
ATGAGATGATCAAGTCTGGT+AGG + chr3_4:22890595-22890614 Msa0494620:CDS 40.0%
CAATCACCAATACCCTTGTA+GGG - chr3_4:22890630-22890649 None:intergenic 40.0%
ACAATCACCAATACCCTTGT+AGG - chr3_4:22890631-22890650 None:intergenic 40.0%
TAAAAGAACAATGGCCGATG+AGG + chr3_4:22890653-22890672 Msa0494620:CDS 40.0%
AAAAGAACAATGGCCGATGA+GGG + chr3_4:22890654-22890673 Msa0494620:CDS 40.0%
! TATCCGTTATTTCCCTACTC+AGG + chr3_4:22890713-22890732 Msa0494620:CDS 40.0%
GAAAATGGCTAACCTGAGTA+GGG - chr3_4:22890728-22890747 None:intergenic 40.0%
AGAAAATGGCTAACCTGAGT+AGG - chr3_4:22890729-22890748 None:intergenic 40.0%
! GAGGCTTTTCAACTTCAAGA+AGG + chr3_4:22890857-22890876 Msa0494620:CDS 40.0%
GTAAACAAACAAGAGGGATG+AGG - chr3_4:22890943-22890962 None:intergenic 40.0%
AATCATTGGCAAGACGAGTA+CGG - chr3_4:22890980-22890999 None:intergenic 40.0%
TCGTCTTGCCAATGATTCTA+AGG + chr3_4:22890983-22891002 Msa0494620:CDS 40.0%
CTTAGCTGCCTTAGAATCAT+TGG - chr3_4:22890994-22891013 None:intergenic 40.0%
!! GATTCTAAGGCAGCTAAGAA+AGG + chr3_4:22890996-22891015 Msa0494620:CDS 40.0%
AAGAAGACTCTTGCTACTGA+TGG + chr3_4:22891056-22891075 Msa0494620:CDS 40.0%
TTGCTACTGATGGAATTGCT+GGG + chr3_4:22891066-22891085 Msa0494620:CDS 40.0%
GTTGGAATCATTGTTTACCG+TGG + chr3_4:22891116-22891135 Msa0494620:CDS 40.0%
!! AGTGAGAAGAACTGGTTTGA+GGG - chr3_4:22891165-22891184 None:intergenic 40.0%
TCTTCTCACTGGAAAATTGC+AGG + chr3_4:22891175-22891194 Msa0494620:CDS 40.0%
TTCTCCTAACAGTGTCAATG+GGG - chr3_4:22891403-22891422 None:intergenic 40.0%
CTTCTCCTAACAGTGTCAAT+GGG - chr3_4:22891404-22891423 None:intergenic 40.0%
TCTTCTCCTAACAGTGTCAA+TGG - chr3_4:22891405-22891424 None:intergenic 40.0%
CCTCATTCTTGAGGATTTGT+TGG - chr3_4:22891481-22891500 None:intergenic 40.0%
CCAACAAATCCTCAAGAATG+AGG + chr3_4:22891478-22891497 Msa0494620:CDS 40.0%
GGTGCTAAGTCATTGTTCAA+GGG + chr3_4:22891500-22891519 Msa0494620:CDS 40.0%
AAGTTGCAGGTTCTTGTGTT+CGG + chr3_4:22891581-22891600 Msa0494620:CDS 40.0%
CTTGTGTTCGGCAAGAAATA+CGG + chr3_4:22891593-22891612 Msa0494620:CDS 40.0%
! GCAGCAATTAGTCTAACTCT+CGG - chr3_4:22891695-22891714 None:intergenic 40.0%
! CATGATTTATTTGGAGTGGC+AGG + chr3_4:22891798-22891817 Msa0494620:three_prime_UTR 40.0%
CAGGATAAGTAACTGTCACT+TGG + chr3_4:22891817-22891836 Msa0494620:three_prime_UTR 40.0%
! GATTTTATATGGGGATGGTG+TGG + chr3_4:22892053-22892072 Msa0494620:three_prime_UTR 40.0%
! CTGGTGAATTTTCTCCAAGA+TGG + chr3_4:22892310-22892329 Msa0494620:three_prime_UTR 40.0%
AGCACAGATTAAGGAGATGA+AGG - chr3_4:22892427-22892446 None:intergenic 40.0%
! ACAGGCCAGGATTTTTCATA+TGG - chr3_4:22892496-22892515 None:intergenic 40.0%
CTTTGTCAATCAACGAAGAC+AGG - chr3_4:22892514-22892533 None:intergenic 40.0%
AGAGAGAGAAATGTGTGTGG+AGG - chr3_4:22889096-22889115 None:intergenic 45.0%
! TCACGAAGGGTTTTTGCGAA+AGG - chr3_4:22889133-22889152 None:intergenic 45.0%
TCGCAAAAACCCTTCGTGAA+AGG + chr3_4:22889134-22889153 Msa0494620:five_prime_UTR 45.0%
GTAGTACTCTCTCTAGTCGT+TGG + chr3_4:22889817-22889836 Msa0494620:intron 45.0%
GAAAGCAACCATGGTTGATC+AGG + chr3_4:22890224-22890243 Msa0494620:exon 45.0%
GCAACCAACAATCATGGAGA+AGG + chr3_4:22890251-22890270 Msa0494620:CDS 45.0%
GTGACCTTCTCCATGATTGT+TGG - chr3_4:22890258-22890277 None:intergenic 45.0%
ACAATCATGGAGAAGGTCAC+TGG + chr3_4:22890258-22890277 Msa0494620:CDS 45.0%
CGGGTCTTTCATCTGGATTT+AGG + chr3_4:22890298-22890317 Msa0494620:CDS 45.0%
AGCGTTTGAGTAGTTCCCAT+AGG - chr3_4:22890370-22890389 None:intergenic 45.0%
! ACGAAATGGCTCTTTTCTGC+TGG - chr3_4:22890483-22890502 None:intergenic 45.0%
!!! AGCAGCAGCAGTTTTGGAAA+CGG - chr3_4:22890538-22890557 None:intergenic 45.0%
CAGACTTGATCATCTCATCC+TGG - chr3_4:22890593-22890612 None:intergenic 45.0%
CAGGATGAGATGATCAAGTC+TGG + chr3_4:22890591-22890610 Msa0494620:CDS 45.0%
GAGAGCAACAATACCCTCAT+CGG - chr3_4:22890670-22890689 None:intergenic 45.0%
!! ATGAGGGTATTGTTGCTCTC+TGG + chr3_4:22890670-22890689 Msa0494620:CDS 45.0%
AACTTCAAGAAGGACAGGGA+TGG + chr3_4:22890867-22890886 Msa0494620:CDS 45.0%
ACAGGGATGGTTACTGGAAA+TGG + chr3_4:22890880-22890899 Msa0494620:CDS 45.0%
! GGTTACTGGAAATGGTTTGC+CGG + chr3_4:22890888-22890907 Msa0494620:CDS 45.0%
! GAAATGGTTTGCCGGTAACT+TGG + chr3_4:22890896-22890915 Msa0494620:CDS 45.0%
ATCATTGGCAAGACGAGTAC+GGG - chr3_4:22890979-22890998 None:intergenic 45.0%
! TCTAAGGCAGCTAAGAAAGG+TGG + chr3_4:22890999-22891018 Msa0494620:CDS 45.0%
CAGCTAAGAAAGGTGGAGAA+AGG + chr3_4:22891006-22891025 Msa0494620:CDS 45.0%
CTTGCTACTGATGGAATTGC+TGG + chr3_4:22891065-22891084 Msa0494620:CDS 45.0%
CACAGGAGATGTTGAAACCA+CGG - chr3_4:22891097-22891116 None:intergenic 45.0%
GGTTTCAACATCTCCTGTGT+TGG + chr3_4:22891098-22891117 Msa0494620:CDS 45.0%
GTTTACCGTGGTCTGTACTT+CGG + chr3_4:22891128-22891147 Msa0494620:CDS 45.0%
ACAATCCGAAGTACAGACCA+CGG - chr3_4:22891136-22891155 None:intergenic 45.0%
!! CAGTGAGAAGAACTGGTTTG+AGG - chr3_4:22891166-22891185 None:intergenic 45.0%
CTCAAACCAGTTCTTCTCAC+TGG + chr3_4:22891164-22891183 Msa0494620:CDS 45.0%
! TTTTTCGCCAGTTTTGCGCT+TGG + chr3_4:22891341-22891360 Msa0494620:CDS 45.0%
CTTGGATGGCTCATTACCAA+TGG + chr3_4:22891359-22891378 Msa0494620:CDS 45.0%
CATACCCCATTGACACTGTT+AGG + chr3_4:22891396-22891415 Msa0494620:CDS 45.0%
CTTGTACTTGACAGCTTCTC+CGG - chr3_4:22891441-22891460 None:intergenic 45.0%
ACTTAGCACCCTCATTCTTG+AGG - chr3_4:22891490-22891509 None:intergenic 45.0%
GGGTGCTAAGTCATTGTTCA+AGG + chr3_4:22891499-22891518 Msa0494620:CDS 45.0%
AAGTCATTGTTCAAGGGAGC+TGG + chr3_4:22891506-22891525 Msa0494620:CDS 45.0%
! TTCTGGTTACGACAAGTTGC+AGG + chr3_4:22891568-22891587 Msa0494620:CDS 45.0%
TTCGGCAAGAAATACGGATC+TGG + chr3_4:22891599-22891618 Msa0494620:CDS 45.0%
CAGATGAAAGACCCGATCGA+CGG - chr3_4:22890293-22890312 None:intergenic 50.0%
!! ATCTTTGGGACATGGCAGGA+TGG - chr3_4:22890344-22890363 None:intergenic 50.0%
!! GAGAATCTTTGGGACATGGC+AGG - chr3_4:22890348-22890367 None:intergenic 50.0%
CGCTGCATTGCAGAATCCAA+TGG + chr3_4:22890386-22890405 Msa0494620:CDS 50.0%
TCAACAGTTGCCCTGCAAGT+GGG - chr3_4:22890414-22890433 None:intergenic 50.0%
ATCAACAGTTGCCCTGCAAG+TGG - chr3_4:22890415-22890434 None:intergenic 50.0%
! AATGGGAGCAGCAGCAGTTT+TGG - chr3_4:22890544-22890563 None:intergenic 50.0%
TAGGCTATCTGAGCCCTACA+AGG + chr3_4:22890614-22890633 Msa0494620:CDS 50.0%
AGGCTATCTGAGCCCTACAA+GGG + chr3_4:22890615-22890634 Msa0494620:CDS 50.0%
! TCTGAGCCCTACAAGGGTAT+TGG + chr3_4:22890621-22890640 Msa0494620:CDS 50.0%
! GGTATTGTTGCTCTCTGGAG+AGG + chr3_4:22890675-22890694 Msa0494620:CDS 50.0%
AGAAGGACAGGGATGGTTAC+TGG + chr3_4:22890874-22890893 Msa0494620:CDS 50.0%
TTTGCCGGTAACTTGGCATC+CGG + chr3_4:22890903-22890922 Msa0494620:CDS 50.0%
GGTGGAGAAAGGCAGTTCAA+TGG + chr3_4:22891017-22891036 Msa0494620:CDS 50.0%
TGGCTCATTACCAATGGTGC+AGG + chr3_4:22891365-22891384 Msa0494620:CDS 50.0%
TGATGCTAGACCTGCACCAT+TGG - chr3_4:22891378-22891397 None:intergenic 50.0%
GGCAAGAAATACGGATCTGG+TGG + chr3_4:22891602-22891621 Msa0494620:CDS 50.0%
TCAATCAACGAAGACAGGCC+AGG - chr3_4:22892509-22892528 None:intergenic 50.0%
ATGAAAGACCCGATCGACGG+TGG - chr3_4:22890290-22890309 None:intergenic 55.0%
CGTCGATCGGGTCTTTCATC+TGG + chr3_4:22890291-22890310 Msa0494620:CDS 55.0%
! TTTGGGACATGGCAGGATGG+TGG - chr3_4:22890341-22890360 None:intergenic 55.0%
AACAGTTGCCCTGCAAGTGG+GGG - chr3_4:22890412-22890431 None:intergenic 55.0%
CAACAGTTGCCCTGCAAGTG+GGG - chr3_4:22890413-22890432 None:intergenic 55.0%
ACCTCCGGATGCCAAGTTAC+CGG - chr3_4:22890910-22890929 None:intergenic 55.0%
GGAATTGCTGGGCTTTACCG+TGG + chr3_4:22891077-22891096 Msa0494620:CDS 55.0%
! TCGCCAGTTTTGCGCTTGGA+TGG + chr3_4:22891345-22891364 Msa0494620:CDS 55.0%
GAGCCATCCAAGCGCAAAAC+TGG - chr3_4:22891351-22891370 None:intergenic 55.0%
AAGCACACCAGCACCAGCAA+CGG - chr3_4:22891549-22891568 None:intergenic 55.0%
! GGACATGGCAGGATGGTGGT+AGG - chr3_4:22890337-22890356 None:intergenic 60.0%
ATCCAATGGCCCCCACTTGC+AGG + chr3_4:22890400-22890419 Msa0494620:CDS 60.0%
TCCAATGGCCCCCACTTGCA+GGG + chr3_4:22890401-22890420 Msa0494620:CDS 60.0%
AACATCCTCCGAGCCGTTGC+TGG + chr3_4:22891533-22891552 Msa0494620:CDS 60.0%
!! GCTGGTGCTGGTGTGCTTTC+TGG + chr3_4:22891551-22891570 Msa0494620:CDS 60.0%
TGGCCAGCTCCACCGTCGAT+CGG + chr3_4:22890278-22890297 Msa0494620:CDS 65.0%
GCTGGAGCAGCGACGAAGAC+AGG - chr3_4:22890465-22890484 None:intergenic 65.0%
GCCGGTAACTTGGCATCCGG+AGG + chr3_4:22890906-22890925 Msa0494620:CDS 65.0%
!! TTGGCATCCGGAGGTGCTGC+TGG + chr3_4:22890915-22890934 Msa0494620:CDS 65.0%
! TGAGGCACCAGCAGCACCTC+CGG - chr3_4:22890925-22890944 None:intergenic 65.0%
CACCAGCACCAGCAACGGCT+CGG - chr3_4:22891544-22891563 None:intergenic 65.0%
GGCCAGCTCCACCGTCGATC+GGG + chr3_4:22890279-22890298 Msa0494620:CDS 70.0%
GACCCGATCGACGGTGGAGC+TGG - chr3_4:22890284-22890303 None:intergenic 70.0%
GCCCTGCAAGTGGGGGCCAT+TGG - chr3_4:22890405-22890424 None:intergenic 70.0%
! CAGCACCAGCAACGGCTCGG+AGG - chr3_4:22891541-22891560 None:intergenic 70.0%
CTCCGAGCCGTTGCTGGTGC+TGG + chr3_4:22891539-22891558 Msa0494620:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 22889078 22892607 22889078 ID=Msa0494620;Name=Msa0494620
chr3_4 mRNA 22889078 22892607 22889078 ID=Msa0494620-mRNA-1;Parent=Msa0494620;Name=Msa0494620-mRNA-1;_AED=0.05;_eAED=0.05;_QI=88|1|1|1|0.66|0.5|4|976|389
chr3_4 exon 22889078 22889155 22889078 ID=Msa0494620-mRNA-1:exon:5682;Parent=Msa0494620-mRNA-1
chr3_4 exon 22890224 22890734 22890224 ID=Msa0494620-mRNA-1:exon:5683;Parent=Msa0494620-mRNA-1
chr3_4 exon 22890825 22891196 22890825 ID=Msa0494620-mRNA-1:exon:5684;Parent=Msa0494620-mRNA-1
chr3_4 exon 22891335 22892607 22891335 ID=Msa0494620-mRNA-1:exon:5685;Parent=Msa0494620-mRNA-1
chr3_4 five_prime_UTR 22889078 22889155 22889078 ID=Msa0494620-mRNA-1:five_prime_utr;Parent=Msa0494620-mRNA-1
chr3_4 five_prime_UTR 22890224 22890233 22890224 ID=Msa0494620-mRNA-1:five_prime_utr;Parent=Msa0494620-mRNA-1
chr3_4 CDS 22890234 22890734 22890234 ID=Msa0494620-mRNA-1:cds;Parent=Msa0494620-mRNA-1
chr3_4 CDS 22890825 22891196 22890825 ID=Msa0494620-mRNA-1:cds;Parent=Msa0494620-mRNA-1
chr3_4 CDS 22891335 22891631 22891335 ID=Msa0494620-mRNA-1:cds;Parent=Msa0494620-mRNA-1
chr3_4 three_prime_UTR 22891632 22892607 22891632 ID=Msa0494620-mRNA-1:three_prime_utr;Parent=Msa0494620-mRNA-1
Gene Sequence

>Msa0494620

ATGGTTGATCAGGTTAAGCAACCAACAATCATGGAGAAGGTCACTGGCCAGCTCCACCGTCGATCGGGTCTTTCATCTGGATTTAGGGGTTATGAAGGAACCTACCACCATCCTGCCATGTCCCAAAGATTCTCCTATGGGAACTACTCAAACGCTGCATTGCAGAATCCAATGGCCCCCACTTGCAGGGCAACTGTTGATTTGTCAGCTGTTGCAACAACTGCTTCTCCTGTCTTCGTCGCTGCTCCAGCAGAAAAGAGCCATTTCGTTATTGACTTTCTCATGGGTGGTGTTTCAGCTGCCGTTTCCAAAACTGCTGCTGCTCCCATTGAACGTATTAAGCTTTTGATCCAAAACCAGGATGAGATGATCAAGTCTGGTAGGCTATCTGAGCCCTACAAGGGTATTGGTGATTGTTTTAAAAGAACAATGGCCGATGAGGGTATTGTTGCTCTCTGGAGAGGAAACACTGCCAATGTTATCCGTTATTTCCCTACTCAGGCTTTGAACTTTGCATTCAAAGATTACTTCAAGAGGCTTTTCAACTTCAAGAAGGACAGGGATGGTTACTGGAAATGGTTTGCCGGTAACTTGGCATCCGGAGGTGCTGCTGGTGCCTCATCCCTCTTGTTTGTTTACTCTCTTGATTATGCCCGTACTCGTCTTGCCAATGATTCTAAGGCAGCTAAGAAAGGTGGAGAAAGGCAGTTCAATGGTCTTGTTGATGTCTACAAGAAGACTCTTGCTACTGATGGAATTGCTGGGCTTTACCGTGGTTTCAACATCTCCTGTGTTGGAATCATTGTTTACCGTGGTCTGTACTTCGGATTGTATGATTCCCTCAAACCAGTTCTTCTCACTGGAAAATTGCAGGATAGCTTTTTCGCCAGTTTTGCGCTTGGATGGCTCATTACCAATGGTGCAGGTCTAGCATCATACCCCATTGACACTGTTAGGAGAAGAATGATGATGACATCCGGAGAAGCTGTCAAGTACAAGAGTTCCTTTGATGCATTCCAACAAATCCTCAAGAATGAGGGTGCTAAGTCATTGTTCAAGGGAGCTGGTGCTAACATCCTCCGAGCCGTTGCTGGTGCTGGTGTGCTTTCTGGTTACGACAAGTTGCAGGTTCTTGTGTTCGGCAAGAAATACGGATCTGGTGGTGCTTAA
Protein sequence

>Msa0494620

MVDQVKQPTIMEKVTGQLHRRSGLSSGFRGYEGTYHHPAMSQRFSYGNYSNAALQNPMAPTCRATVDLSAVATTASPVFVAAPAEKSHFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKGIGDCFKRTMADEGIVALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLASGGAAGASSLLFVYSLDYARTRLANDSKAAKKGGERQFNGLVDVYKKTLATDGIAGLYRGFNISCVGIIVYRGLYFGLYDSLKPVLLTGKLQDSFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQILKNEGAKSLFKGAGANILRAVAGAGVLSGYDKLQVLVFGKKYGSGGA*