AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0448390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0448390 MtrunA17_Chr3g0090511 92.565 269 19 1 1 268 68 336 1.56e-167 466
Msa0448390 MtrunA17_Chr1g0185421 49.446 271 124 5 1 268 1 261 6.11e-69 214
Msa0448390 MtrunA17_Chr1g0205331 47.566 267 126 5 1 266 32 285 1.69e-66 208
Msa0448390 MtrunA17_Chr1g0191151 41.455 275 116 5 1 267 1 238 1.58e-41 142
Msa0448390 MtrunA17_Chr2g0315671 39.416 137 76 3 1 137 71 200 1.67e-22 94.0
Msa0448390 MtrunA17_Chr2g0315661 40.336 119 65 2 1 119 1 113 3.43e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0448390 AT3G22500.1 45.489 266 131 5 1 266 1 252 2.46e-56 181
Msa0448390 AT3G22490.1 43.173 271 127 5 5 266 6 258 1.15e-55 180
Msa0448390 AT1G03120.1 50.382 131 60 1 137 267 56 181 1.14e-29 110
Msa0448390 AT5G27980.1 53.465 101 47 0 139 239 68 168 5.51e-25 98.6
Msa0448390 AT5G53260.1 41.406 128 70 1 140 267 50 172 1.13e-17 79.0
Msa0448390 AT5G53270.1 42.063 126 68 1 140 265 31 151 1.83e-16 75.1
Msa0448390 AT1G03120.2 53.333 75 35 0 137 211 56 130 1.37e-14 69.3

Find 118 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTTGTGCCTATGAATA+TGG 0.166083 3_3:-28138588 Msa0448390:CDS
CCATGGTTCTTCATGTTTCC+TGG 0.238117 3_3:+28136904 None:intergenic
CACCGTATTTGATGGGTTCT+TGG 0.267760 3_3:+28138912 None:intergenic
GTGTGAGGCCTGGCGGTGTC+GGG 0.275992 3_3:-28138424 Msa0448390:CDS
TGTGTGAGGCCTGGCGGTGT+CGG 0.283706 3_3:-28138425 Msa0448390:CDS
ATGGCTGCAGCAGGACTTCC+TGG 0.305505 3_3:+28138788 None:intergenic
GCCCTATGAAGCTTTGGTTT+TGG 0.307458 3_3:+28136459 None:intergenic
GGCCCATGAAGCTTTGGTTT+TGG 0.323674 3_3:+28136690 None:intergenic
CTCTCAACAGCCACGCTAAT+CGG 0.330105 3_3:-28138379 Msa0448390:CDS
CCATCAGTGCTGCTGAGATT+AGG 0.336376 3_3:-28138463 Msa0448390:CDS
TGCCAAAACCAAAGCTTCAT+AGG 0.346487 3_3:-28136461 Msa0448390:three_prime_UTR
GCCATGTTAGCGGCCACTTG+TGG 0.347945 3_3:+28138845 None:intergenic
TTGGTTGAACGGTAGTTAGC+TGG 0.354791 3_3:-28136494 Msa0448390:three_prime_UTR
TTGGTTGAACGGTAGTTAGC+TGG 0.354791 3_3:-28136730 Msa0448390:three_prime_UTR
CATCAGTGCTGCTGAGATTA+GGG 0.357619 3_3:-28138462 Msa0448390:CDS
CCATGTTAGCGGCCACTTGT+GGG 0.359786 3_3:+28138846 None:intergenic
AAAACTTTCTGATGTGTTAA+CGG 0.360481 3_3:-28138342 Msa0448390:intron
GCCGCTTGCATGGCTGCAGC+AGG 0.364075 3_3:+28138779 None:intergenic
TATGTTGGCCCATGAAGCTT+TGG 0.373622 3_3:+28136684 None:intergenic
CACTTTGTGCCTATGAATAT+GGG 0.375801 3_3:-28138587 Msa0448390:CDS
GCCTGGCGGTGTCGGGGCGA+CGG 0.386365 3_3:-28138417 Msa0448390:CDS
ATGCCAAAACCAAAGCTTCA+TGG 0.386758 3_3:-28136693 Msa0448390:three_prime_UTR
GCCGTCGCCCCGACACCGCC+AGG 0.387638 3_3:+28138416 None:intergenic
GAAAACATCACCGTATTTGA+TGG 0.395353 3_3:+28138904 None:intergenic
ATGTTGGCCCTATGAAGCTT+TGG 0.400785 3_3:+28136453 None:intergenic
CGGATAAGCTGGTGGAGGAC+AGG 0.403743 3_3:-28137058 Msa0448390:CDS
GTTAATCTCTTCTTGAAGCC+TGG 0.405950 3_3:+28138934 None:intergenic
GCGGTTGATGGTCAGATTAT+AGG 0.409172 3_3:-28138632 Msa0448390:CDS
CTTCTTGAAGCCTGGTTGGT+TGG 0.415684 3_3:+28138942 None:intergenic
GTTGTGACGGAGGCGGTTGA+TGG 0.427340 3_3:-28138644 Msa0448390:CDS
GAAGCCTGGTTGGTTGGCCA+TGG 0.430327 3_3:+28138948 None:intergenic
GCCTATGAATATGGGCTTTG+CGG 0.430604 3_3:-28138579 Msa0448390:CDS
GTTAGAAGTGGCGGGGATAT+CGG 0.431798 3_3:-28138522 Msa0448390:CDS
TAGAACAGCAATTTCAAACT+AGG 0.434039 3_3:+28136570 None:intergenic
TAGAACAGCAATTTCAAACT+AGG 0.434039 3_3:+28136806 None:intergenic
ATAGGCACAAAGTGCGCGTC+AGG 0.451108 3_3:+28138596 None:intergenic
ATCTCTTCTTGAAGCCTGGT+TGG 0.457290 3_3:+28138938 None:intergenic
GATATCGGTTGGTGATAAAC+CGG 0.458559 3_3:-28138507 Msa0448390:CDS
GATCATTGCGGCCGCTTGCA+TGG 0.469213 3_3:+28138769 None:intergenic
TCGTCATGGTATAGCAGAAA+TGG 0.469856 3_3:-28138717 Msa0448390:CDS
TCTTTGGTTGAAGAAGGGTT+TGG 0.477120 3_3:+28138990 None:intergenic
GGAGAGAGGTGTGTGAGGCC+TGG 0.480169 3_3:-28138434 Msa0448390:CDS
ATCACATTAATTCTATATGT+TGG 0.484111 3_3:+28136669 None:intergenic
CCCACAAGTGGCCGCTAACA+TGG 0.487849 3_3:-28138846 Msa0448390:CDS
CCTAATCTCAGCAGCACTGA+TGG 0.492034 3_3:+28138463 None:intergenic
AACCAAGAACCCATCAAATA+CGG 0.492643 3_3:-28138914 Msa0448390:CDS
ATAGGATGCGGCGGATAAGC+TGG 0.493945 3_3:-28137069 Msa0448390:intron
CGTTCAACCAAGAAAATCAA+TGG 0.496664 3_3:+28136506 None:intergenic
CGTTCAACCAAGAAAATCAA+TGG 0.496664 3_3:+28136742 None:intergenic
TTCTGCTGCAGCCATGTTAG+CGG 0.499845 3_3:+28138835 None:intergenic
TCCTGCTGCAGCCATGCAAG+CGG 0.501385 3_3:-28138780 Msa0448390:CDS
TGGTTGAACGGTAGTTAGCT+GGG 0.502681 3_3:-28136493 Msa0448390:three_prime_UTR
TGGTTGAACGGTAGTTAGCT+GGG 0.502681 3_3:-28136729 Msa0448390:three_prime_UTR
TGCCAAAACCAAAGCTTCAT+GGG 0.502813 3_3:-28136692 Msa0448390:three_prime_UTR
GCCGCAAAGCCCATATTCAT+AGG 0.505709 3_3:+28138578 None:intergenic
GAGCTTAGCTGTTGTGGCCA+TGG 0.506595 3_3:+28136929 None:intergenic
CAATGGCTCGTTGGTCGTCA+TGG 0.509926 3_3:-28138731 Msa0448390:CDS
GGATAAGCTGGTGGAGGACA+GGG 0.510419 3_3:-28137057 Msa0448390:CDS
CTGGTTGAGCTTAGCTGTTG+TGG 0.511900 3_3:+28136923 None:intergenic
TGTGAGGCCTGGCGGTGTCG+GGG 0.521665 3_3:-28138423 Msa0448390:CDS
AAAACATCACCGTATTTGAT+GGG 0.527182 3_3:+28138905 None:intergenic
GCCAAAACCAAAGCTTCATA+GGG 0.528808 3_3:-28136460 Msa0448390:three_prime_UTR
TGGTGGAATAGCTGCATCCA+TGG 0.532996 3_3:-28136946 Msa0448390:CDS
GGAGAGGCGTTAGAAGTGGC+GGG 0.533131 3_3:-28138530 Msa0448390:CDS
AGAACAGCAATTTCAAACTA+GGG 0.533994 3_3:+28136571 None:intergenic
AGAACAGCAATTTCAAACTA+GGG 0.533994 3_3:+28136807 None:intergenic
AGGCCTCACACACCTCTCTC+CGG 0.534996 3_3:+28138436 None:intergenic
TGCCGACCGTCGTGTTGTGA+CGG 0.537709 3_3:-28138657 Msa0448390:CDS
GAAGGGTTTGGAAATTTGAG+TGG 0.550972 3_3:+28139002 None:intergenic
TCAGCATCGTTCTGATCCAC+CGG 0.561215 3_3:+28138488 None:intergenic
GTTTGGAAATTTGAGTGGAG+TGG 0.563850 3_3:+28139007 None:intergenic
GCTGCTGAGATTAGGGCTAC+CGG 0.568023 3_3:-28138455 Msa0448390:CDS
CATGTTAGCGGCCACTTGTG+GGG 0.571385 3_3:+28138847 None:intergenic
CTCCGTCACAACACGACGGT+CGG 0.572167 3_3:+28138655 None:intergenic
CCGTCGTGTTGTGACGGAGG+CGG 0.578384 3_3:-28138651 Msa0448390:CDS
GCATCTGCACAATGGCTCGT+TGG 0.578751 3_3:-28138740 Msa0448390:CDS
GATGACGATCCGATCACTAT+CGG 0.581623 3_3:-28138551 Msa0448390:CDS
AATATTCATCATTTGTTGTG+TGG 0.590823 3_3:+28136977 None:intergenic
CGGAGAGGCGTTAGAAGTGG+CGG 0.590961 3_3:-28138531 Msa0448390:CDS
ACCACATTGACTCTATATGT+TGG 0.591209 3_3:+28136437 None:intergenic
AACGCCTCTCCGATAGTGAT+CGG 0.591968 3_3:+28138542 None:intergenic
TGCGGCGGATAAGCTGGTGG+AGG 0.592783 3_3:-28137063 Msa0448390:CDS
TGAGCCATGGCCAACCAACC+AGG 0.605511 3_3:-28138952 Msa0448390:CDS
TGAGACAATCTCCCCACAAG+TGG 0.610093 3_3:-28138858 Msa0448390:CDS
GAAGTGGCGGGGATATCGGT+TGG 0.611879 3_3:-28138518 Msa0448390:CDS
CACAACAGCTAAGCTCAACC+AGG 0.614854 3_3:-28136922 Msa0448390:CDS
TATCGGAGAGGCGTTAGAAG+TGG 0.617007 3_3:-28138534 Msa0448390:CDS
AGATTAGGGCTACCGGAGAG+AGG 0.625834 3_3:-28138448 Msa0448390:CDS
TAATTGCAAATAAACTCACT+AGG 0.625907 3_3:+28136536 None:intergenic
TAATTGCAAATAAACTCACT+AGG 0.625907 3_3:+28136772 None:intergenic
ATGATGAATATTGCTGAACC+TGG 0.626149 3_3:-28136966 Msa0448390:CDS
ATGGATGCAGCTATTCCACC+AGG 0.630413 3_3:+28136948 None:intergenic
ATGAATATTGCTGAACCTGG+TGG 0.635922 3_3:-28136963 Msa0448390:CDS
TAAGCTGGTGGAGGACAGGG+CGG 0.636080 3_3:-28137054 Msa0448390:CDS
GGTTGAACGGTAGTTAGCTG+GGG 0.636122 3_3:-28136492 Msa0448390:three_prime_UTR
GGTTGAACGGTAGTTAGCTG+GGG 0.636122 3_3:-28136728 Msa0448390:three_prime_UTR
ATCGGTTGGTGATAAACCGG+TGG 0.639207 3_3:-28138504 Msa0448390:CDS
AAACACAACACAATGAGCCA+TGG 0.639243 3_3:-28138965 Msa0448390:exon
CTACCGGAGAGAGGTGTGTG+AGG 0.640214 3_3:-28138439 Msa0448390:CDS
TGTATATATATAGGATGCGG+CGG 0.642444 3_3:-28137078 Msa0448390:intron
ATTAGCGTGGCTGTTGAGAG+CGG 0.645143 3_3:+28138382 None:intergenic
CGACCGTCGTGTTGTGACGG+AGG 0.646821 3_3:-28138654 Msa0448390:CDS
TCAGATTATAGGACGGCGCG+TGG 0.654489 3_3:-28138621 Msa0448390:CDS
AGGAAATTAGTAAAAGACCA+TGG 0.665507 3_3:+28136887 None:intergenic
TCAACGAGGCATCTGCACAA+TGG 0.666804 3_3:-28138748 Msa0448390:CDS
TCTCTTCAATCCGATTAGCG+TGG 0.674286 3_3:+28138369 None:intergenic
AGAAGCTGTGTATGATGCAG+AGG 0.680635 3_3:-28137015 Msa0448390:CDS
GAGAGGCGTTAGAAGTGGCG+GGG 0.680725 3_3:-28138529 Msa0448390:CDS
CCAGGAAACATGAAGAACCA+TGG 0.680740 3_3:-28136904 Msa0448390:exon
AGATGCCTCGTTGATCATTG+CGG 0.682722 3_3:+28138757 None:intergenic
TTGATGGTCAGATTATAGGA+CGG 0.688715 3_3:-28138628 Msa0448390:CDS
GAGAGGTGTGTGAGGCCTGG+CGG 0.694862 3_3:-28138431 Msa0448390:CDS
GCCAACATATAGAGTCAATG+TGG 0.705397 3_3:-28136438 Msa0448390:three_prime_UTR
GGATGCGGCGGATAAGCTGG+TGG 0.713312 3_3:-28137066 Msa0448390:CDS
CGATCCGATCACTATCGGAG+AGG 0.731692 3_3:-28138546 Msa0448390:CDS
AGCGGCCGCAATGATCAACG+AGG 0.739216 3_3:-28138762 Msa0448390:CDS
CCGCCTCCGTCACAACACGA+CGG 0.744880 3_3:+28138651 None:intergenic
TGTTCGTGTGACAGAAACAA+CGG 0.764152 3_3:-28138681 Msa0448390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAAGATTATAATTGATT+AGG - chr3_3:28137167-28137186 Msa0448390:intron 15.0%
!! TTTAACTAACTAATCAAAAA+AGG + chr3_3:28137194-28137213 None:intergenic 15.0%
!!! CATGTTATTATTATGTTTTT+TGG - chr3_3:28137400-28137419 Msa0448390:intron 15.0%
!! AATTTAGTGAAATTGTAATA+TGG - chr3_3:28138041-28138060 Msa0448390:intron 15.0%
!! TTAACTAAAATATATGTTGA+AGG - chr3_3:28138065-28138084 Msa0448390:intron 15.0%
!! TAACTAAAATATATGTTGAA+GGG - chr3_3:28138066-28138085 Msa0448390:intron 15.0%
!!! CTTATTTTAACAAAACAAAA+AGG + chr3_3:28138567-28138586 None:intergenic 15.0%
!!! CTTATTTTAACAAAACAAAA+AGG + chr3_3:28138567-28138586 None:intergenic 15.0%
!!! TGATTAGTTAGTTAAATACT+TGG - chr3_3:28137198-28137217 Msa0448390:intron 20.0%
!!! TTTATTTTTTAGTGTGTGTT+TGG - chr3_3:28137554-28137573 Msa0448390:intron 20.0%
!! TGTAGAATTGAAATTTCATT+TGG - chr3_3:28138179-28138198 Msa0448390:intron 20.0%
!!! AAATATTACAAAGTACTCAT+TGG + chr3_3:28138323-28138342 None:intergenic 20.0%
!!! GAGTACTTTGTAATATTTTT+TGG - chr3_3:28138325-28138344 Msa0448390:intron 20.0%
!!! TACTTTGTAATATTTTTTGG+TGG - chr3_3:28138328-28138347 Msa0448390:intron 20.0%
!! ATCACATTAATTCTATATGT+TGG + chr3_3:28138765-28138784 None:intergenic 20.0%
!!! TTTTTTCTTTGGTTGAAGAA+GGG + chr3_3:28136449-28136468 None:intergenic 25.0%
!!! ATTGTGTTGTGTTTTTTCTT+TGG + chr3_3:28136460-28136479 None:intergenic 25.0%
! AAAACTTTCTGATGTGTTAA+CGG - chr3_3:28137089-28137108 Msa0448390:intron 25.0%
! TTAGTTAGTTAAATACTTGG+AGG - chr3_3:28137201-28137220 Msa0448390:intron 25.0%
!!! CCAATTTGTAGCTTTATATA+TGG + chr3_3:28137468-28137487 None:intergenic 25.0%
! CCATATATAAAGCTACAAAT+TGG - chr3_3:28137465-28137484 Msa0448390:intron 25.0%
! TGTTTGGTATTATGGATTTA+CGG - chr3_3:28137570-28137589 Msa0448390:intron 25.0%
! TTGATGAGCAAAATTGATAT+AGG - chr3_3:28138009-28138028 Msa0448390:intron 25.0%
!!! AATATTCATCATTTGTTGTG+TGG + chr3_3:28138457-28138476 None:intergenic 25.0%
! TAATTGCAAATAAACTCACT+AGG + chr3_3:28138662-28138681 None:intergenic 25.0%
! TAATTGCAAATAAACTCACT+AGG + chr3_3:28138662-28138681 None:intergenic 25.0%
!!! GTTTTTTCTTTGGTTGAAGA+AGG + chr3_3:28136450-28136469 None:intergenic 30.0%
AAAACATCACCGTATTTGAT+GGG + chr3_3:28136529-28136548 None:intergenic 30.0%
AAGCACTTATAGTGAATGTA+AGG - chr3_3:28137234-28137253 Msa0448390:intron 30.0%
GATATATAATAACGTGCGTT+TGG + chr3_3:28137500-28137519 None:intergenic 30.0%
TTAGTGTGTGTTTGGTATTA+TGG - chr3_3:28137562-28137581 Msa0448390:intron 30.0%
TCTTAGTGTGTTTGGTATTA+TGG - chr3_3:28137692-28137711 Msa0448390:intron 30.0%
GAAACCATATATAAGCAACT+CGG - chr3_3:28137778-28137797 Msa0448390:intron 30.0%
GTAGTGTTTGTTTGGTATTA+CGG - chr3_3:28137838-28137857 Msa0448390:intron 30.0%
!! GTGTAGCTTTTGAAAAATCA+CGG - chr3_3:28137912-28137931 Msa0448390:intron 30.0%
GTATCAATTTGTCAGAATCT+CGG + chr3_3:28137945-28137964 None:intergenic 30.0%
ATGAGCAAAATTGATATAGG+TGG - chr3_3:28138012-28138031 Msa0448390:intron 30.0%
!! TTGGTGGTGTGTATATATAT+AGG - chr3_3:28138344-28138363 Msa0448390:CDS 30.0%
GTGTGTATATATATAGGATG+CGG - chr3_3:28138350-28138369 Msa0448390:CDS 30.0%
AGGAAATTAGTAAAAGACCA+TGG + chr3_3:28138547-28138566 None:intergenic 30.0%
AGAACAGCAATTTCAAACTA+GGG + chr3_3:28138627-28138646 None:intergenic 30.0%
TAGAACAGCAATTTCAAACT+AGG + chr3_3:28138628-28138647 None:intergenic 30.0%
!!! CATTGATTTTCTTGGTTGAA+CGG - chr3_3:28138690-28138709 Msa0448390:CDS 30.0%
AGAACAGCAATTTCAAACTA+GGG + chr3_3:28138627-28138646 None:intergenic 30.0%
TAGAACAGCAATTTCAAACT+AGG + chr3_3:28138628-28138647 None:intergenic 30.0%
!!! CATTGATTTTCTTGGTTGAA+CGG - chr3_3:28138690-28138709 Msa0448390:CDS 30.0%
AACCAAGAACCCATCAAATA+CGG - chr3_3:28136517-28136536 Msa0448390:three_prime_UTR 35.0%
GAAAACATCACCGTATTTGA+TGG + chr3_3:28136530-28136549 None:intergenic 35.0%
! CAGCAGAATTCAACGTTTTA+GGG - chr3_3:28136611-28136630 Msa0448390:three_prime_UTR 35.0%
! TTGATGGTCAGATTATAGGA+CGG - chr3_3:28136803-28136822 Msa0448390:three_prime_UTR 35.0%
CACTTTGTGCCTATGAATAT+GGG - chr3_3:28136844-28136863 Msa0448390:three_prime_UTR 35.0%
! TAAGTGTCTGTTCGTTTTAG+TGG + chr3_3:28137526-28137545 None:intergenic 35.0%
TTGGTATTATGGATTTACGG+TGG - chr3_3:28137573-28137592 Msa0448390:intron 35.0%
TGGTATTATGGATTTACGGT+GGG - chr3_3:28137574-28137593 Msa0448390:intron 35.0%
GTGTAGCTTCTACAATATCA+CGG + chr3_3:28137623-28137642 None:intergenic 35.0%
TAATCAACTTAGTGCGTGTT+TGG - chr3_3:28137667-28137686 Msa0448390:intron 35.0%
GTTTGGTATCTTAGTGTGTT+TGG - chr3_3:28137684-28137703 Msa0448390:intron 35.0%
AGTGTGTTTGGTATTATGGT+AGG - chr3_3:28137696-28137715 Msa0448390:intron 35.0%
!! ATATATAAGCAACTCGGTGT+CGG - chr3_3:28137784-28137803 Msa0448390:intron 35.0%
TGTTTGGTACTCAGTGTATT+TGG - chr3_3:28137811-28137830 Msa0448390:intron 35.0%
TTGGTATCGTAGTGTTTGTT+TGG - chr3_3:28137830-28137849 Msa0448390:intron 35.0%
AAGCAAAGCTAGAATCAAAG+TGG + chr3_3:28137967-28137986 None:intergenic 35.0%
TCTCTGCTGGAAAATGTAAT+TGG + chr3_3:28138241-28138260 None:intergenic 35.0%
!! TCCGTAAAAGAGTACTAACT+AGG - chr3_3:28138274-28138293 Msa0448390:intron 35.0%
! GCCTAGTTAGTACTCTTTTA+CGG + chr3_3:28138278-28138297 None:intergenic 35.0%
TGTATATATATAGGATGCGG+CGG - chr3_3:28138353-28138372 Msa0448390:CDS 35.0%
ATGATGAATATTGCTGAACC+TGG - chr3_3:28138465-28138484 Msa0448390:CDS 35.0%
!!! GTTGTAGCCATTGATTTTCT+TGG - chr3_3:28138682-28138701 Msa0448390:CDS 35.0%
CGTTCAACCAAGAAAATCAA+TGG + chr3_3:28138692-28138711 None:intergenic 35.0%
!!! GTTGTAGCCATTGATTTTCT+TGG - chr3_3:28138682-28138701 Msa0448390:CDS 35.0%
CGTTCAACCAAGAAAATCAA+TGG + chr3_3:28138692-28138711 None:intergenic 35.0%
!! ACCACATTGACTCTATATGT+TGG + chr3_3:28138997-28139016 None:intergenic 35.0%
GTTTGGAAATTTGAGTGGAG+TGG + chr3_3:28136427-28136446 None:intergenic 40.0%
GAAGGGTTTGGAAATTTGAG+TGG + chr3_3:28136432-28136451 None:intergenic 40.0%
TCTTTGGTTGAAGAAGGGTT+TGG + chr3_3:28136444-28136463 None:intergenic 40.0%
AAACACAACACAATGAGCCA+TGG - chr3_3:28136466-28136485 Msa0448390:three_prime_UTR 40.0%
GTTAATCTCTTCTTGAAGCC+TGG + chr3_3:28136500-28136519 None:intergenic 40.0%
! GCAGCAGAATTCAACGTTTT+AGG - chr3_3:28136610-28136629 Msa0448390:three_prime_UTR 40.0%
TCGTCATGGTATAGCAGAAA+TGG - chr3_3:28136714-28136733 Msa0448390:three_prime_UTR 40.0%
TGTTCGTGTGACAGAAACAA+CGG - chr3_3:28136750-28136769 Msa0448390:three_prime_UTR 40.0%
GCACTTTGTGCCTATGAATA+TGG - chr3_3:28136843-28136862 Msa0448390:three_prime_UTR 40.0%
!! GATATCGGTTGGTGATAAAC+CGG - chr3_3:28136924-28136943 Msa0448390:CDS 40.0%
!! TGTCTGTTCGTTTTAGTGGT+AGG + chr3_3:28137522-28137541 None:intergenic 40.0%
CGGTGGGTATACCATAATTA+CGG - chr3_3:28137590-28137609 Msa0448390:intron 40.0%
ACGGTAGATGACCGTAATTA+TGG + chr3_3:28137604-28137623 None:intergenic 40.0%
GACACCGAGTTGCTTATATA+TGG + chr3_3:28137785-28137804 None:intergenic 40.0%
ACGACAGATAACTGTGATTC+TGG + chr3_3:28137872-28137891 None:intergenic 40.0%
CTTTCATGACTAATCTCTGC+TGG + chr3_3:28138254-28138273 None:intergenic 40.0%
ATGAATATTGCTGAACCTGG+TGG - chr3_3:28138468-28138487 Msa0448390:CDS 40.0%
ATGCCAAAACCAAAGCTTCA+TGG - chr3_3:28138738-28138757 Msa0448390:CDS 40.0%
TGCCAAAACCAAAGCTTCAT+GGG - chr3_3:28138739-28138758 Msa0448390:CDS 40.0%
TGCCAAAACCAAAGCTTCAT+AGG - chr3_3:28138739-28138758 Msa0448390:CDS 40.0%
GCCAAAACCAAAGCTTCATA+GGG - chr3_3:28138971-28138990 Msa0448390:exon 40.0%
GCCAACATATAGAGTCAATG+TGG - chr3_3:28138993-28139012 Msa0448390:five_prime_UTR 40.0%
ATCTCTTCTTGAAGCCTGGT+TGG + chr3_3:28136496-28136515 None:intergenic 45.0%
!! CACCGTATTTGATGGGTTCT+TGG + chr3_3:28136522-28136541 None:intergenic 45.0%
! GGTGATGTTTTCAACGTCTC+TGG - chr3_3:28136538-28136557 Msa0448390:three_prime_UTR 45.0%
AGATGCCTCGTTGATCATTG+CGG + chr3_3:28136677-28136696 None:intergenic 45.0%
!! GCGGTTGATGGTCAGATTAT+AGG - chr3_3:28136799-28136818 Msa0448390:three_prime_UTR 45.0%
GCCTATGAATATGGGCTTTG+CGG - chr3_3:28136852-28136871 Msa0448390:three_prime_UTR 45.0%
GATGACGATCCGATCACTAT+CGG - chr3_3:28136880-28136899 Msa0448390:three_prime_UTR 45.0%
! CATCAGTGCTGCTGAGATTA+GGG - chr3_3:28136969-28136988 Msa0448390:CDS 45.0%
TCTCTTCAATCCGATTAGCG+TGG + chr3_3:28137065-28137084 None:intergenic 45.0%
TGGTAGGTATGCCAGAATCA+CGG - chr3_3:28137712-28137731 Msa0448390:intron 45.0%
GAGTACTAACTAGGCTGTCT+AGG - chr3_3:28138283-28138302 Msa0448390:intron 45.0%
AGAAGCTGTGTATGATGCAG+AGG - chr3_3:28138416-28138435 Msa0448390:CDS 45.0%
CCATGGTTCTTCATGTTTCC+TGG + chr3_3:28138530-28138549 None:intergenic 45.0%
CCAGGAAACATGAAGAACCA+TGG - chr3_3:28138527-28138546 Msa0448390:CDS 45.0%
TTGGTTGAACGGTAGTTAGC+TGG - chr3_3:28138701-28138720 Msa0448390:CDS 45.0%
TGGTTGAACGGTAGTTAGCT+GGG - chr3_3:28138702-28138721 Msa0448390:CDS 45.0%
TATGTTGGCCCATGAAGCTT+TGG + chr3_3:28138750-28138769 None:intergenic 45.0%
TTGGTTGAACGGTAGTTAGC+TGG - chr3_3:28138701-28138720 Msa0448390:CDS 45.0%
TGGTTGAACGGTAGTTAGCT+GGG - chr3_3:28138702-28138721 Msa0448390:CDS 45.0%
!! GCCCTATGAAGCTTTGGTTT+TGG + chr3_3:28138975-28138994 None:intergenic 45.0%
ATGTTGGCCCTATGAAGCTT+TGG + chr3_3:28138981-28139000 None:intergenic 45.0%
CTTCTTGAAGCCTGGTTGGT+TGG + chr3_3:28136492-28136511 None:intergenic 50.0%
TGAGACAATCTCCCCACAAG+TGG - chr3_3:28136573-28136592 Msa0448390:three_prime_UTR 50.0%
TTCTGCTGCAGCCATGTTAG+CGG + chr3_3:28136599-28136618 None:intergenic 50.0%
!!! GTTTTAGGGCATGCACAACC+AGG - chr3_3:28136625-28136644 Msa0448390:three_prime_UTR 50.0%
! TCAACGAGGCATCTGCACAA+TGG - chr3_3:28136683-28136702 Msa0448390:three_prime_UTR 50.0%
GCCGCAAAGCCCATATTCAT+AGG + chr3_3:28136856-28136875 None:intergenic 50.0%
AACGCCTCTCCGATAGTGAT+CGG + chr3_3:28136892-28136911 None:intergenic 50.0%
!! TATCGGAGAGGCGTTAGAAG+TGG - chr3_3:28136897-28136916 Msa0448390:exon 50.0%
! GTTAGAAGTGGCGGGGATAT+CGG - chr3_3:28136909-28136928 Msa0448390:exon 50.0%
!! ATCGGTTGGTGATAAACCGG+TGG - chr3_3:28136927-28136946 Msa0448390:CDS 50.0%
TCAGCATCGTTCTGATCCAC+CGG + chr3_3:28136946-28136965 None:intergenic 50.0%
! CCTAATCTCAGCAGCACTGA+TGG + chr3_3:28136971-28136990 None:intergenic 50.0%
! CCATCAGTGCTGCTGAGATT+AGG - chr3_3:28136968-28136987 Msa0448390:CDS 50.0%
ATTAGCGTGGCTGTTGAGAG+CGG + chr3_3:28137052-28137071 None:intergenic 50.0%
CTCTCAACAGCCACGCTAAT+CGG - chr3_3:28137052-28137071 Msa0448390:CDS 50.0%
ACGACAGATGACCGTGATTC+TGG + chr3_3:28137726-28137745 None:intergenic 50.0%
ATGGATGCAGCTATTCCACC+AGG + chr3_3:28138486-28138505 None:intergenic 50.0%
TGGTGGAATAGCTGCATCCA+TGG - chr3_3:28138485-28138504 Msa0448390:CDS 50.0%
CTGGTTGAGCTTAGCTGTTG+TGG + chr3_3:28138511-28138530 None:intergenic 50.0%
CACAACAGCTAAGCTCAACC+AGG - chr3_3:28138509-28138528 Msa0448390:CDS 50.0%
GGTTGAACGGTAGTTAGCTG+GGG - chr3_3:28138703-28138722 Msa0448390:CDS 50.0%
!! GGCCCATGAAGCTTTGGTTT+TGG + chr3_3:28138744-28138763 None:intergenic 50.0%
GGTTGAACGGTAGTTAGCTG+GGG - chr3_3:28138703-28138722 Msa0448390:CDS 50.0%
CATGTTAGCGGCCACTTGTG+GGG + chr3_3:28136587-28136606 None:intergenic 55.0%
CCATGTTAGCGGCCACTTGT+GGG + chr3_3:28136588-28136607 None:intergenic 55.0%
GCATCTGCACAATGGCTCGT+TGG - chr3_3:28136691-28136710 Msa0448390:three_prime_UTR 55.0%
CAATGGCTCGTTGGTCGTCA+TGG - chr3_3:28136700-28136719 Msa0448390:three_prime_UTR 55.0%
TCAGATTATAGGACGGCGCG+TGG - chr3_3:28136810-28136829 Msa0448390:three_prime_UTR 55.0%
ATAGGCACAAAGTGCGCGTC+AGG + chr3_3:28136838-28136857 None:intergenic 55.0%
CGATCCGATCACTATCGGAG+AGG - chr3_3:28136885-28136904 Msa0448390:three_prime_UTR 55.0%
GCTGCTGAGATTAGGGCTAC+CGG - chr3_3:28136976-28136995 Msa0448390:CDS 55.0%
AGATTAGGGCTACCGGAGAG+AGG - chr3_3:28136983-28137002 Msa0448390:CDS 55.0%
!! ACTCGGTGTCGGTGTGTGTT+TGG - chr3_3:28137795-28137814 Msa0448390:intron 55.0%
ATAGGATGCGGCGGATAAGC+TGG - chr3_3:28138362-28138381 Msa0448390:CDS 55.0%
GGATAAGCTGGTGGAGGACA+GGG - chr3_3:28138374-28138393 Msa0448390:CDS 55.0%
GAGCTTAGCTGTTGTGGCCA+TGG + chr3_3:28138505-28138524 None:intergenic 55.0%
TGAGCCATGGCCAACCAACC+AGG - chr3_3:28136479-28136498 Msa0448390:three_prime_UTR 60.0%
! GAAGCCTGGTTGGTTGGCCA+TGG + chr3_3:28136486-28136505 None:intergenic 60.0%
CCCACAAGTGGCCGCTAACA+TGG - chr3_3:28136585-28136604 Msa0448390:three_prime_UTR 60.0%
GCCATGTTAGCGGCCACTTG+TGG + chr3_3:28136589-28136608 None:intergenic 60.0%
ATGGCTGCAGCAGGACTTCC+TGG + chr3_3:28136646-28136665 None:intergenic 60.0%
TCCTGCTGCAGCCATGCAAG+CGG - chr3_3:28136651-28136670 Msa0448390:three_prime_UTR 60.0%
GATCATTGCGGCCGCTTGCA+TGG + chr3_3:28136665-28136684 None:intergenic 60.0%
AGCGGCCGCAATGATCAACG+AGG - chr3_3:28136669-28136688 Msa0448390:three_prime_UTR 60.0%
TGCCGACCGTCGTGTTGTGA+CGG - chr3_3:28136774-28136793 Msa0448390:three_prime_UTR 60.0%
CTCCGTCACAACACGACGGT+CGG + chr3_3:28136779-28136798 None:intergenic 60.0%
! GTTGTGACGGAGGCGGTTGA+TGG - chr3_3:28136787-28136806 Msa0448390:three_prime_UTR 60.0%
!! CGGAGAGGCGTTAGAAGTGG+CGG - chr3_3:28136900-28136919 Msa0448390:exon 60.0%
!! GGAGAGGCGTTAGAAGTGGC+GGG - chr3_3:28136901-28136920 Msa0448390:exon 60.0%
!! GAGAGGCGTTAGAAGTGGCG+GGG - chr3_3:28136902-28136921 Msa0448390:exon 60.0%
! GAAGTGGCGGGGATATCGGT+TGG - chr3_3:28136913-28136932 Msa0448390:exon 60.0%
! CTACCGGAGAGAGGTGTGTG+AGG - chr3_3:28136992-28137011 Msa0448390:CDS 60.0%
AGGCCTCACACACCTCTCTC+CGG + chr3_3:28136998-28137017 None:intergenic 60.0%
CGGATAAGCTGGTGGAGGAC+AGG - chr3_3:28138373-28138392 Msa0448390:CDS 60.0%
TAAGCTGGTGGAGGACAGGG+CGG - chr3_3:28138377-28138396 Msa0448390:CDS 60.0%
CGACCGTCGTGTTGTGACGG+AGG - chr3_3:28136777-28136796 Msa0448390:three_prime_UTR 65.0%
CCGCCTCCGTCACAACACGA+CGG + chr3_3:28136783-28136802 None:intergenic 65.0%
! CCGTCGTGTTGTGACGGAGG+CGG - chr3_3:28136780-28136799 Msa0448390:three_prime_UTR 65.0%
! GGAGAGAGGTGTGTGAGGCC+TGG - chr3_3:28136997-28137016 Msa0448390:CDS 65.0%
! GAGAGGTGTGTGAGGCCTGG+CGG - chr3_3:28137000-28137019 Msa0448390:CDS 65.0%
TGTGTGAGGCCTGGCGGTGT+CGG - chr3_3:28137006-28137025 Msa0448390:CDS 65.0%
GGATGCGGCGGATAAGCTGG+TGG - chr3_3:28138365-28138384 Msa0448390:CDS 65.0%
TGCGGCGGATAAGCTGGTGG+AGG - chr3_3:28138368-28138387 Msa0448390:CDS 65.0%
GCCGCTTGCATGGCTGCAGC+AGG + chr3_3:28136655-28136674 None:intergenic 70.0%
GTGTGAGGCCTGGCGGTGTC+GGG - chr3_3:28137007-28137026 Msa0448390:CDS 70.0%
! TGTGAGGCCTGGCGGTGTCG+GGG - chr3_3:28137008-28137027 Msa0448390:CDS 70.0%
!! GCCTGGCGGTGTCGGGGCGA+CGG - chr3_3:28137014-28137033 Msa0448390:CDS 80.0%
! GCCGTCGCCCCGACACCGCC+AGG + chr3_3:28137018-28137037 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 28136423 28139030 28136423 ID=Msa0448390;Name=Msa0448390
chr3_3 mRNA 28136423 28139030 28136423 ID=Msa0448390-mRNA-1;Parent=Msa0448390;Name=Msa0448390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=55|1|1|1|1|1|2|491|268
chr3_3 exon 28136423 28137087 28136423 ID=Msa0448390-mRNA-1:exon:5454;Parent=Msa0448390-mRNA-1
chr3_3 exon 28138343 28139030 28138343 ID=Msa0448390-mRNA-1:exon:5453;Parent=Msa0448390-mRNA-1
chr3_3 five_prime_UTR 28138976 28139030 28138976 ID=Msa0448390-mRNA-1:five_prime_utr;Parent=Msa0448390-mRNA-1
chr3_3 CDS 28138343 28138975 28138343 ID=Msa0448390-mRNA-1:cds;Parent=Msa0448390-mRNA-1
chr3_3 CDS 28136914 28137087 28136914 ID=Msa0448390-mRNA-1:cds;Parent=Msa0448390-mRNA-1
chr3_3 three_prime_UTR 28136423 28136913 28136423 ID=Msa0448390-mRNA-1:three_prime_utr;Parent=Msa0448390-mRNA-1
Gene Sequence

>Msa0448390

ATGAGCCATGGCCAACCAACCAGGCTTCAAGAAGAGATTAACCAAGAACCCATCAAATACGGTGATGTTTTCAACGTCTCTGGTGATTTAGCATCTGAGACAATCTCCCCACAAGTGGCCGCTAACATGGCTGCAGCAGAATTCAACGTTTTAGGGCATGCACAACCAGGAAGTCCTGCTGCAGCCATGCAAGCGGCCGCAATGATCAACGAGGCATCTGCACAATGGCTCGTTGGTCGTCATGGTATAGCAGAAATGGTCAAAGATCATGCTGTTCGTGTGACAGAAACAACGGCTGCCGACCGTCGTGTTGTGACGGAGGCGGTTGATGGTCAGATTATAGGACGGCGCGTGGAGCCTGACGCGCACTTTGTGCCTATGAATATGGGCTTTGCGGCTATAGATGACGATCCGATCACTATCGGAGAGGCGTTAGAAGTGGCGGGGATATCGGTTGGTGATAAACCGGTGGATCAGAACGATGCTGATGCCATCAGTGCTGCTGAGATTAGGGCTACCGGAGAGAGGTGTGTGAGGCCTGGCGGTGTCGGGGCGACGGCGCAATCTGCAGCCGCTCTCAACAGCCACGCTAATCGGATTGAAGAGATGACAAAACTTTCTGATGTGTTAACGGATGCGGCGGATAAGCTGGTGGAGGACAGGGCGGTGACGAAAGAAGATGCAGAAGCTGTGTATGATGCAGAGGTGCATAAGAATTCATCCACACAACAAATGATGAATATTGCTGAACCTGGTGGAATAGCTGCATCCATGGCCACAACAGCTAAGCTCAACCAGGAAACATGA
Protein sequence

>Msa0448390

MSHGQPTRLQEEINQEPIKYGDVFNVSGDLASETISPQVAANMAAAEFNVLGHAQPGSPAAAMQAAAMINEASAQWLVGRHGIAEMVKDHAVRVTETTAADRRVVTEAVDGQIIGRRVEPDAHFVPMNMGFAAIDDDPITIGEALEVAGISVGDKPVDQNDADAISAAEIRATGERCVRPGGVGATAQSAAALNSHANRIEEMTKLSDVLTDAADKLVEDRAVTKEDAEAVYDAEVHKNSSTQQMMNIAEPGGIAASMATTAKLNQET*