Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0449850 | XP_013461791.1 | 72.249 | 209 | 28 | 2 | 1 | 179 | 1 | 209 | 1.50e-87 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0449850 | A0A072V2E5 | 72.249 | 209 | 28 | 2 | 1 | 179 | 1 | 209 | 7.15e-88 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0357220 | Msa0449850 | 0.943851 | 5.700982e-103 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0449850 | MtrunA17_Chr3g0134881 | 72.249 | 209 | 28 | 2 | 1 | 179 | 1 | 209 | 1.38e-91 | 266 |
| Msa0449850 | MtrunA17_Chr1g0152461 | 44.279 | 201 | 86 | 8 | 1 | 179 | 1 | 197 | 8.23e-44 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0449850 | AT1G59590.1 | 35.135 | 185 | 91 | 6 | 19 | 179 | 8 | 187 | 8.17e-25 | 96.3 |
| Msa0449850 | AT1G10220.1 | 33.708 | 178 | 89 | 6 | 19 | 169 | 4 | 179 | 6.97e-16 | 72.4 |
| Msa0449850 | AT1G10220.2 | 31.746 | 189 | 89 | 6 | 19 | 169 | 4 | 190 | 3.48e-14 | 68.2 |
Find 25 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGAACTATCTTTCAAAAT+AGG | 0.247880 | 3_3:-34750696 | None:intergenic |
| GGAAATTGCCACAAACTAAA+TGG | 0.257314 | 3_3:-34750157 | None:intergenic |
| AGAGAAATTGATGAAGAAAA+AGG | 0.259298 | 3_3:+34750722 | Msa0449850:CDS |
| TTTGATCAAGTTCAGATAGA+AGG | 0.288309 | 3_3:-34750295 | None:intergenic |
| CCAAAAGAAAAGACAACAAT+AGG | 0.309829 | 3_3:+34750228 | Msa0449850:CDS |
| ATGGTATATCATCTTCTAAT+TGG | 0.359629 | 3_3:-34750184 | None:intergenic |
| ATGCTTAACCATTTAGTTTG+TGG | 0.373237 | 3_3:+34750149 | Msa0449850:CDS |
| TCAAAGCCTCTTGAAGCATA+AGG | 0.385538 | 3_3:-34750260 | None:intergenic |
| TTGTTGTTGTTGACTGTGTT+TGG | 0.422356 | 3_3:+34750627 | Msa0449850:CDS |
| TCAACATGTTTATGATCCAT+TGG | 0.448150 | 3_3:-34750462 | None:intergenic |
| ATGGTGAGATATTTCCAACT+TGG | 0.480861 | 3_3:-34750499 | None:intergenic |
| TATCATCTTCTAATTGGTGT+TGG | 0.486872 | 3_3:-34750178 | None:intergenic |
| CAAGAGTGAAGAACATGTTA+AGG | 0.497546 | 3_3:+34750413 | Msa0449850:CDS |
| TATCTGAACTTGATCAAAGA+AGG | 0.501411 | 3_3:+34750300 | Msa0449850:CDS |
| AAAATCAATATCACAACAGC+AGG | 0.519128 | 3_3:-34750606 | None:intergenic |
| CAAAAGAAAAGACAACAATA+GGG | 0.523809 | 3_3:+34750229 | Msa0449850:CDS |
| TGGTGAGATATTTCCAACTT+GGG | 0.526102 | 3_3:-34750498 | None:intergenic |
| TTGATCAAGTTCAGATAGAA+GGG | 0.545038 | 3_3:-34750294 | None:intergenic |
| AGAAACAAAACAACCCAAGT+TGG | 0.556433 | 3_3:+34750485 | Msa0449850:CDS |
| GATAATCCTTATGCTTCAAG+AGG | 0.584005 | 3_3:+34750254 | Msa0449850:CDS |
| CGAACAAAAGATATGTCATG+TGG | 0.588570 | 3_3:-34750347 | None:intergenic |
| CGCTTTGCACATGTATTGTG+TGG | 0.598253 | 3_3:+34750664 | Msa0449850:CDS |
| ACAGCAGGCACATAGAAAGA+AGG | 0.600782 | 3_3:-34750591 | None:intergenic |
| GAACAAAAGATATGTCATGT+GGG | 0.637973 | 3_3:-34750346 | None:intergenic |
| ATATCTCACCATGTTGACGA+TGG | 0.679358 | 3_3:+34750510 | Msa0449850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGGTATATCATCTTCTAAT+TGG | - | chr3_3:34750187-34750206 | None:intergenic | 25.0% |
| !! | TTCTTTTGGATTTGGATTTT+GGG | - | chr3_3:34750217-34750236 | None:intergenic | 25.0% |
| !! | TTTCTTTTGGATTTGGATTT+TGG | - | chr3_3:34750218-34750237 | None:intergenic | 25.0% |
| ! | CAAAAGAAAAGACAACAATA+GGG | + | chr3_3:34750229-34750248 | Msa0449850:CDS | 25.0% |
| !! | AACATCTTTTTATGAACCAA+TGG | + | chr3_3:34750446-34750465 | Msa0449850:CDS | 25.0% |
| ! | TTCGAACTATCTTTCAAAAT+AGG | - | chr3_3:34750699-34750718 | None:intergenic | 25.0% |
| ! | AGAGAAATTGATGAAGAAAA+AGG | + | chr3_3:34750722-34750741 | Msa0449850:CDS | 25.0% |
| !! | ATGCTTAACCATTTAGTTTG+TGG | + | chr3_3:34750149-34750168 | Msa0449850:CDS | 30.0% |
| TATCATCTTCTAATTGGTGT+TGG | - | chr3_3:34750181-34750200 | None:intergenic | 30.0% | |
| ! | GTTGTCTTTTCTTTTGGATT+TGG | - | chr3_3:34750225-34750244 | None:intergenic | 30.0% |
| ! | CCTATTGTTGTCTTTTCTTT+TGG | - | chr3_3:34750231-34750250 | None:intergenic | 30.0% |
| CCAAAAGAAAAGACAACAAT+AGG | + | chr3_3:34750228-34750247 | Msa0449850:CDS | 30.0% | |
| TTGATCAAGTTCAGATAGAA+GGG | - | chr3_3:34750297-34750316 | None:intergenic | 30.0% | |
| TTTGATCAAGTTCAGATAGA+AGG | - | chr3_3:34750298-34750317 | None:intergenic | 30.0% | |
| TATCTGAACTTGATCAAAGA+AGG | + | chr3_3:34750300-34750319 | Msa0449850:CDS | 30.0% | |
| GAACAAAAGATATGTCATGT+GGG | - | chr3_3:34750349-34750368 | None:intergenic | 30.0% | |
| TCAACATGTTTATGATCCAT+TGG | - | chr3_3:34750465-34750484 | None:intergenic | 30.0% | |
| TCAAACAAGATTGAAACCTT+TGG | + | chr3_3:34750543-34750562 | Msa0449850:intron | 30.0% | |
| CATATTCAAAGAGAAACCAA+AGG | - | chr3_3:34750562-34750581 | None:intergenic | 30.0% | |
| TCTCTTTGAATATGTTGAAG+AGG | + | chr3_3:34750568-34750587 | Msa0449850:intron | 30.0% | |
| AAAATCAATATCACAACAGC+AGG | - | chr3_3:34750609-34750628 | None:intergenic | 30.0% | |
| GGAAATTGCCACAAACTAAA+TGG | - | chr3_3:34750160-34750179 | None:intergenic | 35.0% | |
| GATAATCCTTATGCTTCAAG+AGG | + | chr3_3:34750254-34750273 | Msa0449850:CDS | 35.0% | |
| CGAACAAAAGATATGTCATG+TGG | - | chr3_3:34750350-34750369 | None:intergenic | 35.0% | |
| CAAGAGTGAAGAACATGTTA+AGG | + | chr3_3:34750413-34750432 | Msa0449850:CDS | 35.0% | |
| AGAAACAAAACAACCCAAGT+TGG | + | chr3_3:34750485-34750504 | Msa0449850:CDS | 35.0% | |
| TGGTGAGATATTTCCAACTT+GGG | - | chr3_3:34750501-34750520 | None:intergenic | 35.0% | |
| ATGGTGAGATATTTCCAACT+TGG | - | chr3_3:34750502-34750521 | None:intergenic | 35.0% | |
| ! | CATTTTTACCATCGTCAACA+TGG | - | chr3_3:34750521-34750540 | None:intergenic | 35.0% |
| ! | TTGTTGTTGTTGACTGTGTT+TGG | + | chr3_3:34750627-34750646 | Msa0449850:CDS | 35.0% |
| ! | TTGGATTTTGGGCTTGAACA+TGG | - | chr3_3:34750206-34750225 | None:intergenic | 40.0% |
| TCAAAGCCTCTTGAAGCATA+AGG | - | chr3_3:34750263-34750282 | None:intergenic | 40.0% | |
| ATATCTCACCATGTTGACGA+TGG | + | chr3_3:34750510-34750529 | Msa0449850:CDS | 40.0% | |
| ACAGCAGGCACATAGAAAGA+AGG | - | chr3_3:34750594-34750613 | None:intergenic | 45.0% | |
| CGCTTTGCACATGTATTGTG+TGG | + | chr3_3:34750664-34750683 | Msa0449850:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 34750149 | 34750749 | 34750149 | ID=Msa0449850;Name=Msa0449850 |
| chr3_3 | mRNA | 34750149 | 34750749 | 34750149 | ID=Msa0449850-mRNA-1;Parent=Msa0449850;Name=Msa0449850-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|0|0|3|0|179 |
| chr3_3 | exon | 34750149 | 34750368 | 34750149 | ID=Msa0449850-mRNA-1:exon:6183;Parent=Msa0449850-mRNA-1 |
| chr3_3 | exon | 34750385 | 34750539 | 34750385 | ID=Msa0449850-mRNA-1:exon:6184;Parent=Msa0449850-mRNA-1 |
| chr3_3 | exon | 34750588 | 34750749 | 34750588 | ID=Msa0449850-mRNA-1:exon:6185;Parent=Msa0449850-mRNA-1 |
| chr3_3 | CDS | 34750149 | 34750368 | 34750149 | ID=Msa0449850-mRNA-1:cds;Parent=Msa0449850-mRNA-1 |
| chr3_3 | CDS | 34750385 | 34750539 | 34750385 | ID=Msa0449850-mRNA-1:cds;Parent=Msa0449850-mRNA-1 |
| chr3_3 | CDS | 34750588 | 34750749 | 34750588 | ID=Msa0449850-mRNA-1:cds;Parent=Msa0449850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
