AlfalfaGEDB | Msa0357220

M. sativa cultivar zhongmu-4 / Msa0357220

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0357220	XP_013461791.1	74.163	209	29	2	1	184	1	209	4.17e-91	276

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0357220	A0A072V2E5	74.163	209	29	2	1	184	1	209	1.99e-91	276

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0092410	Msa0357220	0.803139	4.019607e-49	-8.615850e-47
Msa0178510	Msa0357220	0.800355	1.498455e-48	-8.615850e-47
Msa0190530	Msa0357220	0.825462	4.626522e-54	-8.615850e-47
Msa0201570	Msa0357220	0.806705	7.225414e-50	-8.615850e-47
Msa0244140	Msa0357220	0.801586	8.397877e-49	-8.615850e-47
Msa0244190	Msa0357220	0.811890	5.582484e-51	-8.615850e-47
Msa0347750	Msa0357220	0.806984	6.306917e-50	-8.615850e-47
Msa0357220	Msa0389390	0.800241	1.580424e-48	-8.615850e-47
Msa0357220	Msa0398200	0.803855	2.856759e-49	-8.615850e-47
Msa0357220	Msa0449850	0.943851	5.700982e-103	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0357220	MtrunA17_Chr3g0134881	74.163	209	29	2	1	184	1	209	3.83e-95	276
Msa0357220	MtrunA17_Chr1g0152461	43.902	205	86	8	1	184	1	197	3.31e-43	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0357220	AT1G59590.1	34.409	186	96	5	19	184	8	187	6.82e-27	101
Msa0357220	AT1G10220.1	34.270	178	93	6	19	174	4	179	4.41e-17	75.9
Msa0357220	AT1G10220.2	32.275	189	93	6	19	174	4	190	1.86e-15	71.6

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Find 25 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAATATCACAACAGC+AGG	0.519128	3_1:-38487400	None:intergenic
ACAGCAGGCACATAGAAAGA+AGG	0.600782	3_1:-38487385	None:intergenic
AGAAACAAAACAACCCAAGT+TGG	0.556433	3_1:+38487279	Msa0357220:CDS
AGAGAAATTGATGAAGAAAA+AGG	0.259298	3_1:+38487516	Msa0357220:CDS
ATATCTCACCATGTTGACGA+TGG	0.679358	3_1:+38487304	Msa0357220:CDS
ATGCTTAACCATTTAGTTTG+TGG	0.373237	3_1:+38486943	Msa0357220:CDS
ATGGTATATCATCTTCTAAT+TGG	0.359629	3_1:-38486978	None:intergenic
ATGGTGAGATATTTCCAACT+TGG	0.480861	3_1:-38487293	None:intergenic
CAAAAGAAAAGACAACAATA+GGG	0.523809	3_1:+38487023	Msa0357220:CDS
CAAGAGTGAAGAACATGTTA+AGG	0.497546	3_1:+38487207	Msa0357220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATCATCTTCTAATTGGTGT+TGG	-	chr3_1:38486975-38486994	None:intergenic	30.0%
CCAAAAGAAAAGACAACAAT+AGG	+	chr3_1:38487022-38487041	Msa0357220:CDS	30.0%
TTGATCAAGTTCAGATAGAA+GGG	-	chr3_1:38487091-38487110	None:intergenic	30.0%
TTTGATCAAGTTCAGATAGA+AGG	-	chr3_1:38487092-38487111	None:intergenic	30.0%
TATCTGAACTTGATCAAAGA+AGG	+	chr3_1:38487094-38487113	Msa0357220:CDS	30.0%
GAACAAAAGATATGTCATGT+GGG	-	chr3_1:38487143-38487162	None:intergenic	30.0%
TCAACATGTTTATGATCCAT+TGG	-	chr3_1:38487259-38487278	None:intergenic	30.0%
TCAAACAAGATTGTAAGCTT+TGG	+	chr3_1:38487337-38487356	Msa0357220:intron	30.0%
TCTCTTTGAATATGTTGAAG+AGG	+	chr3_1:38487362-38487381	Msa0357220:intron	30.0%
AAAATCAATATCACAACAGC+AGG	-	chr3_1:38487403-38487422	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	38486943	38487543	38486943	ID=Msa0357220;Name=Msa0357220
chr3_1	mRNA	38486943	38487543	38486943	ID=Msa0357220-mRNA-1;Parent=Msa0357220;Name=Msa0357220-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0\|0\|1\|0\|0\|3\|0\|184
chr3_1	exon	38486943	38487162	38486943	ID=Msa0357220-mRNA-1:exon:6950;Parent=Msa0357220-mRNA-1
chr3_1	exon	38487179	38487348	38487179	ID=Msa0357220-mRNA-1:exon:6951;Parent=Msa0357220-mRNA-1
chr3_1	exon	38487382	38487543	38487382	ID=Msa0357220-mRNA-1:exon:6952;Parent=Msa0357220-mRNA-1
chr3_1	CDS	38486943	38487162	38486943	ID=Msa0357220-mRNA-1:cds;Parent=Msa0357220-mRNA-1
chr3_1	CDS	38487179	38487348	38487179	ID=Msa0357220-mRNA-1:cds;Parent=Msa0357220-mRNA-1
chr3_1	CDS	38487382	38487543	38487382	ID=Msa0357220-mRNA-1:cds;Parent=Msa0357220-mRNA-1

Gene Sequence

>Msa0357220

ATGCTTAACCATTTAGTTTGTGGCAATTTCCAACACCAATTAGAAGATGATATACCATGTTCAAGCCCAAAATCCAAATCCAAAAGAAAAGACAACAATAGGGACGATAATCCTTATGCTTCAAGAGGCTTAGATAAATTTTCTACCCTTCTATCTGAACTTGATCAAAGAAGGAAGAAAGTTTACTCACAAATGAACCCACATGACATATCTTTTGTTCTGATGATTAACAATCATCAGAAGAAACACAAGAGTGAAGAACATGTTAAGGTAAGACATGTAACATCTTTTTATGAACCAATGGATCATAAACATGTTGAAGAAACAAAACAACCCAAGTTGGAAATATCTCACCATGTTGACGATGGTAAAAATGTATCAAACAAGATTAGGCCTTCTTTCTATGTGCCTGCTGTTGTGATATTGATTTTGTTGTTGTTGACTGTGTTTGGAAGATCATTTGCATCGCTTTGCACATGTATTGTGTGGTATATAGTTCCTATTTTGAAAGATAGTTCGAAAGAAGAGAAATTGATGAAGAAAAAGGATTAT

Protein sequence

>Msa0357220

MLNHLVCGNFQHQLEDDIPCSSPKSKSKRKDNNRDDNPYASRGLDKFSTLLSELDQRRKKVYSQMNPHDISFVLMINNHQKKHKSEEHVKVRHVTSFYEPMDHKHVEETKQPKLEISHHVDDGKNVSNKIRPSFYVPAVVILILLLLTVFGRSFASLCTCIVWYIVPILKDSSKEEKLMKKKDY