AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0450130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0450130 MtrunA17_Chr3g0118331 93.995 383 23 0 4 386 190 572 0.0 726
Msa0450130 MtrunA17_Chr3g0118321 80.637 377 73 0 3 379 190 566 0.0 643
Msa0450130 MtrunA17_Chr3g0118351 72.174 345 72 2 65 386 1 344 0.0 510
Msa0450130 MtrunA17_Chr8g0382691 48.062 387 185 5 4 383 197 574 6.59e-121 362
Msa0450130 MtrunA17_Chr3g0118931 77.320 97 22 0 290 386 10 106 1.05e-50 166
Msa0450130 MtrunA17_Chr5g0404361 33.923 339 201 9 37 358 257 589 1.46e-43 160
Msa0450130 MtrunA17_Chr5g0429511 33.819 343 202 10 35 359 202 537 2.16e-41 153
Msa0450130 MtrunA17_Chr5g0404341 32.229 332 206 8 42 359 219 545 4.25e-40 149
Msa0450130 MtrunA17_Chr5g0436461 33.939 330 200 8 42 358 208 532 1.21e-38 145
Msa0450130 MtrunA17_Chr5g0437681 29.917 361 212 11 26 358 198 545 2.59e-38 144
Msa0450130 MtrunA17_Chr3g0104061 33.526 346 202 11 29 359 203 535 2.63e-38 144
Msa0450130 MtrunA17_Chr4g0031901 34.203 345 182 15 42 354 228 559 3.15e-38 144
Msa0450130 MtrunA17_Chr2g0278811 33.531 337 190 12 48 358 257 585 3.30e-38 145
Msa0450130 MtrunA17_Chr7g0238941 31.700 347 190 10 42 354 219 552 4.95e-37 141
Msa0450130 MtrunA17_Chr3g0114211 29.348 368 221 12 26 368 207 560 8.16e-37 140
Msa0450130 MtrunA17_Chr7g0237491 31.579 342 204 8 42 356 218 556 1.01e-36 140
Msa0450130 MtrunA17_Chr3g0133861 33.438 320 183 11 63 358 5 318 1.62e-36 135
Msa0450130 MtrunA17_Chr7g0238981 32.022 356 211 9 42 369 232 584 2.74e-36 139
Msa0450130 MtrunA17_Chr7g0237441 31.594 345 196 11 42 354 218 554 7.41e-36 138
Msa0450130 MtrunA17_Chr5g0431581 32.335 334 198 11 42 355 210 535 1.02e-35 137
Msa0450130 MtrunA17_Chr7g0238351 31.378 341 203 8 42 354 218 555 6.59e-35 135
Msa0450130 MtrunA17_Chr4g0005921 30.838 334 207 8 42 358 209 535 1.33e-34 134
Msa0450130 MtrunA17_Chr8g0346581 30.347 346 197 11 42 358 219 549 4.14e-33 130
Msa0450130 MtrunA17_Chr8g0374271 29.143 350 215 12 48 383 211 541 6.98e-31 123
Msa0450130 MtrunA17_Chr5g0402831 27.697 343 212 8 48 376 211 531 2.60e-30 122
Msa0450130 MtrunA17_Chr7g0240991 28.219 365 222 12 42 376 208 562 2.71e-29 119
Msa0450130 MtrunA17_Chr4g0038671 26.648 364 234 8 26 376 190 533 7.22e-29 117
Msa0450130 MtrunA17_Chr7g0240971 26.271 354 210 12 42 358 209 548 1.37e-27 114
Msa0450130 MtrunA17_Chr8g0374261 25.071 351 223 10 48 381 210 537 3.49e-27 112
Msa0450130 MtrunA17_Chr3g0079321 27.136 398 226 12 5 376 193 552 6.74e-27 112
Msa0450130 MtrunA17_Chr1g0197301 26.586 331 185 9 48 353 5 302 1.07e-26 108
Msa0450130 MtrunA17_Chr3g0121171 25.613 367 238 11 30 381 196 542 8.00e-26 110
Msa0450130 MtrunA17_Chr3g0121171 25.341 367 239 11 30 381 771 1117 2.36e-25 108
Msa0450130 MtrunA17_Chr3g0121171 25.341 367 239 11 30 381 1912 2258 9.36e-25 107
Msa0450130 MtrunA17_Chr3g0121171 25.068 367 240 11 30 381 1346 1692 2.25e-24 105
Msa0450130 MtrunA17_Chr3g0121151 25.341 367 239 11 30 381 236 582 8.59e-26 109
Msa0450130 MtrunA17_Chr4g0024861 27.548 363 217 11 34 376 195 531 9.42e-26 108
Msa0450130 MtrunA17_Chr4g0008521 26.801 347 217 11 42 358 211 550 1.55e-25 108
Msa0450130 MtrunA17_Chr3g0121161 25.341 367 239 11 30 381 236 582 3.41e-25 107
Msa0450130 MtrunA17_Chr3g0143671 25.496 353 227 10 42 379 206 537 6.51e-25 106
Msa0450130 MtrunA17_Chr4g0056511 26.329 395 241 12 1 376 187 550 7.40e-25 106
Msa0450130 MtrunA17_Chr4g0043251 25.068 367 240 11 30 381 197 543 1.01e-24 105
Msa0450130 MtrunA17_Chr4g0043261 25.815 368 231 12 33 381 193 537 1.29e-24 105
Msa0450130 MtrunA17_Chr4g0075071 25.424 354 222 9 44 379 209 538 1.58e-24 105
Msa0450130 MtrunA17_Chr4g0024641 27.089 347 211 10 48 376 132 454 2.38e-24 105
Msa0450130 MtrunA17_Chr2g0322441 27.346 373 221 12 26 376 190 534 6.75e-24 103
Msa0450130 MtrunA17_Chr1g0157341 26.099 364 230 11 34 380 197 538 1.73e-23 102
Msa0450130 MtrunA17_Chr7g0234061 26.575 365 232 14 40 387 215 560 2.85e-22 98.6
Msa0450130 MtrunA17_Chr5g0436431 37.589 141 84 2 199 338 91 228 3.14e-20 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0450130 AT5G21105.1 64.249 386 137 1 4 388 203 588 0.0 535
Msa0450130 AT5G21105.2 64.249 386 137 1 4 388 12 397 0.0 535
Msa0450130 AT5G21100.1 58.290 386 156 4 4 385 186 570 4.52e-166 477
Msa0450130 AT5G21105.3 64.688 337 118 1 3 338 202 538 1.25e-160 462
Msa0450130 AT4G39830.4 47.583 393 181 8 3 380 92 474 1.71e-121 360
Msa0450130 AT4G39830.3 47.583 393 181 8 3 380 103 485 2.33e-121 360
Msa0450130 AT4G39830.2 47.583 393 181 8 3 380 105 487 2.99e-121 360
Msa0450130 AT4G39830.1 47.704 392 180 8 4 380 201 582 4.94e-120 360
Msa0450130 AT5G03260.1 33.824 340 200 10 37 358 203 535 1.03e-40 151
Msa0450130 AT5G05390.1 30.199 351 205 10 37 358 204 543 4.69e-39 147
Msa0450130 AT5G58910.1 32.249 338 201 10 42 358 171 501 1.45e-37 142
Msa0450130 AT5G58910.3 32.249 338 201 10 42 358 214 544 1.70e-37 142
Msa0450130 AT5G48100.1 31.195 343 202 10 42 358 207 541 1.81e-37 142
Msa0450130 AT5G58910.2 32.249 338 201 10 42 358 215 545 2.01e-37 142
Msa0450130 AT5G09360.1 33.828 337 192 11 42 358 222 547 1.79e-36 140
Msa0450130 AT5G01050.1 30.000 350 202 12 42 358 207 546 4.75e-36 139
Msa0450130 AT2G30210.1 31.014 345 197 11 44 358 215 548 6.99e-36 138
Msa0450130 AT5G01040.1 29.598 348 204 9 42 358 207 544 1.02e-35 138
Msa0450130 AT2G29130.1 34.211 342 187 13 42 354 215 547 1.59e-35 137
Msa0450130 AT2G40370.1 28.609 381 221 12 36 373 205 577 1.65e-34 134
Msa0450130 AT5G07130.2 32.132 333 194 10 52 358 136 462 2.64e-34 132
Msa0450130 AT5G07130.3 32.132 333 194 10 52 358 136 462 2.64e-34 132
Msa0450130 AT5G07130.1 32.432 333 193 10 52 358 221 547 5.86e-34 133
Msa0450130 AT3G09220.2 30.294 340 209 11 42 358 114 448 7.08e-34 131
Msa0450130 AT3G09220.1 30.294 340 209 11 42 358 211 545 2.12e-33 131
Msa0450130 AT5G01190.2 30.337 356 213 11 21 358 184 522 1.15e-31 126
Msa0450130 AT5G01190.1 30.337 356 213 11 21 358 198 536 1.60e-31 125
Msa0450130 AT5G60020.3 31.143 350 196 14 42 354 178 519 2.31e-31 125
Msa0450130 AT5G60020.1 30.618 356 190 13 42 354 210 551 2.46e-31 125
Msa0450130 AT2G38080.1 30.631 333 208 10 42 358 211 536 6.89e-31 124
Msa0450130 AT1G21860.1 28.169 355 214 11 42 379 204 534 2.01e-29 119
Msa0450130 AT1G18140.1 28.883 367 213 12 44 373 216 571 2.81e-29 119
Msa0450130 AT2G46570.2 27.434 339 216 11 42 358 132 462 3.07e-29 119
Msa0450130 AT1G75790.1 27.224 371 224 13 30 376 194 542 4.01e-29 119
Msa0450130 AT2G46570.1 27.647 340 214 12 42 358 217 547 5.42e-29 119
Msa0450130 AT4G22010.1 27.545 334 206 9 34 354 194 504 1.55e-28 117
Msa0450130 AT1G55570.1 27.119 354 219 11 41 376 209 541 5.81e-28 115
Msa0450130 AT5G66920.1 26.667 315 202 7 48 353 217 511 4.83e-27 113
Msa0450130 AT1G21850.1 26.857 350 219 11 42 376 204 531 6.28e-27 112
Msa0450130 AT3G13390.1 28.099 363 221 13 32 376 200 540 1.99e-26 111
Msa0450130 AT1G76160.1 25.989 354 224 10 42 379 203 534 3.74e-26 110
Msa0450130 AT5G60020.2 29.706 340 182 13 42 338 210 535 2.20e-24 105
Msa0450130 AT1G41830.1 25.155 322 212 6 42 354 204 505 5.32e-24 103
Msa0450130 AT4G28090.1 26.611 357 219 10 42 379 206 538 5.36e-23 101
Msa0450130 AT2G23630.1 25.648 347 216 13 48 376 211 533 1.12e-22 100
Msa0450130 AT2G23630.2 25.648 347 216 13 48 376 216 538 1.28e-22 100
Msa0450130 AT4G37160.1 22.636 349 232 9 48 380 213 539 1.40e-22 99.8
Msa0450130 AT4G12420.1 24.627 402 249 13 1 381 185 553 2.68e-22 99.4
Msa0450130 AT4G12420.2 24.627 402 249 13 1 381 185 553 2.68e-22 99.4
Msa0450130 AT5G51480.1 26.368 402 242 17 1 381 188 556 3.20e-22 99.0
Msa0450130 AT4G25240.2 25.980 408 249 15 1 388 189 563 3.66e-22 99.0
Msa0450130 AT4G25240.1 25.980 408 249 15 1 388 189 563 3.66e-22 99.0
Msa0450130 AT4G38420.1 24.462 372 245 12 26 379 190 543 5.80e-21 95.1
Msa0450130 AT5G48450.2 23.315 356 239 7 42 381 211 548 6.96e-21 95.1
Msa0450130 AT5G48450.1 23.647 351 234 7 42 376 211 543 6.98e-21 95.1
Msa0450130 AT3G13400.2 23.677 359 241 10 32 376 199 538 2.52e-18 87.0
Msa0450130 AT3G13400.1 23.677 359 241 10 32 376 199 538 2.52e-18 87.0
Msa0450130 AT1G55560.1 22.443 352 237 11 41 376 206 537 1.72e-17 84.7
Msa0450130 AT4G38420.3 24.194 310 204 10 26 322 190 481 1.92e-13 72.0
Msa0450130 AT4G38420.2 24.468 282 183 9 26 294 190 454 3.20e-12 68.2
Msa0450130 AT4G37160.2 21.705 258 170 7 48 294 213 449 9.96e-12 66.6

Find 110 sgRNAs with CRISPR-Local

Find 320 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGAGGGTGTTCATAAAAT+TGG 0.125745 3_3:-35451298 Msa0450130:CDS
AAATCGGTAATTTCACTAAA+TGG 0.144709 3_3:-35451801 Msa0450130:CDS
AAAACACTCTTAGGAGATTA+AGG 0.220574 3_3:+35451180 None:intergenic
TTGGACTGCATTAAGGTTTA+AGG 0.221108 3_3:-35451395 Msa0450130:CDS
CTCAGTTGTGGGTGTTGTTT+AGG 0.244247 3_3:+35451227 None:intergenic
TGTCTGTGGTGTTCCCATTC+TGG 0.244260 3_3:+35451870 None:intergenic
GCCTTCATGAATAATATATA+GGG 0.245236 3_3:-35451037 Msa0450130:three_prime_UTR
TATTATAGTTAAGGATTGTT+AGG 0.255819 3_3:+35451989 None:intergenic
GATTAATTTAATCATGGATA+TGG 0.285778 3_3:-35453065 None:intergenic
TTTAAGTTATCATCACATTA+TGG 0.289067 3_3:-35451136 Msa0450130:three_prime_UTR
CTTATGGTTGGACTGCATTA+AGG 0.292432 3_3:-35451402 Msa0450130:CDS
GCTCCTAAGTAAGGTGTTAT+TGG 0.293518 3_3:+35451751 None:intergenic
GTGATTGGTGGTGGTAATAT+AGG 0.293720 3_3:+35451946 None:intergenic
TATCATCACATTATGGCCTT+TGG 0.294342 3_3:-35451129 Msa0450130:three_prime_UTR
ACTGCAGTTATATTTCCTTA+TGG 0.308341 3_3:-35451418 Msa0450130:CDS
CGCCTTCATGAATAATATAT+AGG 0.309688 3_3:-35451038 Msa0450130:three_prime_UTR
TGGCGCTACTTCGAGAATCT+TGG 0.314451 3_3:+35452839 None:intergenic
AGGCAGACAACCCTGGTGTT+TGG 0.318817 3_3:-35451375 Msa0450130:CDS
CGATTGAGAGCCAGTAGTTT+TGG 0.320504 3_3:+35452046 None:intergenic
GTTGTTGTACTTGGATTAAC+TGG 0.324579 3_3:+35451646 None:intergenic
GGCAGACAACCCTGGTGTTT+GGG 0.333727 3_3:-35451374 Msa0450130:CDS
CCGTTACCCGTTGTTGTACT+TGG 0.339838 3_3:+35451637 None:intergenic
CAGGGTTTGTATTATAGTTA+AGG 0.342158 3_3:+35451980 None:intergenic
CTGAGGGTGTTCATAAAATT+GGG 0.343862 3_3:-35451297 Msa0450130:CDS
ATCATCACATTATGGCCTTT+GGG 0.352012 3_3:-35451128 Msa0450130:three_prime_UTR
CACCACCAATCACTCCCTTA+TGG 0.366354 3_3:-35451936 Msa0450130:CDS
CATTGAACCACATTTGCATC+TGG 0.368806 3_3:-35451338 Msa0450130:CDS
TTAAATATGTCGAAATCAAA+TGG 0.374923 3_3:+35451673 None:intergenic
TGATTGGTGGTGGTAATATA+GGG 0.386257 3_3:+35451947 None:intergenic
GGCGCTACTTCGAGAATCTT+GGG 0.392401 3_3:+35452840 None:intergenic
CTCAACACACAAAGCAAAAT+CGG 0.395873 3_3:-35451817 Msa0450130:CDS
AATCGGTAATTTCACTAAAT+GGG 0.402093 3_3:-35451800 Msa0450130:CDS
AGGTGTTATTGGCAATGTTA+AGG 0.403860 3_3:+35451762 None:intergenic
TATTTGATTGCTCCTAAGTA+AGG 0.405813 3_3:+35451742 None:intergenic
CTTTGAAATTGCATTGAGGC+AGG 0.417474 3_3:+35452880 None:intergenic
AAATCAAATGGGAATCTATC+TGG 0.430172 3_3:+35451685 None:intergenic
ATTTGACACGTGCACCTTTG+AGG 0.440703 3_3:-35451444 Msa0450130:CDS
GATCTTGGTCCGTGTGTAGA+AGG 0.444511 3_3:+35452073 None:intergenic
CAGTTATATTTCCTTATGGT+TGG 0.450961 3_3:-35451414 Msa0450130:CDS
AAACAAATCATTTCCAGAAT+GGG 0.453424 3_3:-35451883 Msa0450130:CDS
ATTATAGTTAAGGATTGTTA+GGG 0.461154 3_3:+35451990 None:intergenic
GGTGTTGTTTAGGAACATCT+TGG 0.466856 3_3:+35451237 None:intergenic
TTAAGGATTGTTAGGGCTTG+TGG 0.473400 3_3:+35451997 None:intergenic
AAAACAAATCATTTCCAGAA+TGG 0.478795 3_3:-35451884 Msa0450130:CDS
CAAAACTACTGGCTCTCAAT+CGG 0.478813 3_3:-35452045 Msa0450130:CDS
GTTAATCCAAGTACAACAAC+GGG 0.479161 3_3:-35451643 Msa0450130:CDS
CTTAATGCAGTCCAACCATA+AGG 0.485250 3_3:+35451403 None:intergenic
ACCACATTTGCATCTGGGAA+TGG 0.487721 3_3:-35451332 Msa0450130:CDS
TAAATATGTCGAAATCAAAT+GGG 0.488708 3_3:+35451674 None:intergenic
TCTATGCTATCAACCGTGAA+TGG 0.489752 3_3:+35452123 None:intergenic
CGCTCGAAATCGTTCCATAA+GGG 0.490554 3_3:+35451922 None:intergenic
AATATAGGGAAAGTTGAGGC+AGG 0.496705 3_3:+35451961 None:intergenic
ACTCTTAGGAGATTAAGGTT+TGG 0.497652 3_3:+35451185 None:intergenic
GCGCTCGAAATCGTTCCATA+AGG 0.499345 3_3:+35451921 None:intergenic
AGCTTAGCTAGTCTCAGTTG+TGG 0.500207 3_3:+35451215 None:intergenic
TCGTTCCATAAGGGAGTGAT+TGG 0.500888 3_3:+35451931 None:intergenic
AAACTACGTGCACCCATTCA+CGG 0.502338 3_3:-35452136 Msa0450130:CDS
CAATGAAATGCCCAAACACC+AGG 0.503898 3_3:+35451364 None:intergenic
GTCCTTAGCTTCTCTCAACT+TGG 0.508173 3_3:-35452781 Msa0450130:CDS
TTGCCAATAACACCTTACTT+AGG 0.510406 3_3:-35451754 Msa0450130:CDS
GCTTAGCTAGTCTCAGTTGT+GGG 0.510640 3_3:+35451216 None:intergenic
ATGAAGGCGAGTTGGACAAA+TGG 0.513487 3_3:+35451052 None:intergenic
ACTTGCAATCCTAATTCTGT+AGG 0.513702 3_3:+35452809 None:intergenic
CCCCTATATATTATTCATGA+AGG 0.517133 3_3:+35451036 None:intergenic
TGTTCACGAAATTAGCTGCT+AGG 0.529978 3_3:+35452907 None:intergenic
AAGATCCCAACCAAAACTAC+TGG 0.534040 3_3:-35452056 Msa0450130:CDS
CCACATTTGCATCTGGGAAT+GGG 0.538253 3_3:-35451331 Msa0450130:CDS
ATTGAACCACATTTGCATCT+GGG 0.538898 3_3:-35451337 Msa0450130:CDS
CCCATTCCCAGATGCAAATG+TGG 0.540042 3_3:+35451331 None:intergenic
TTCCATAAGGGAGTGATTGG+TGG 0.540746 3_3:+35451934 None:intergenic
TATTATTCATGAAGGCGAGT+TGG 0.544595 3_3:+35451044 None:intergenic
AGCTTGCTCATCAATGGAAG+AGG 0.544734 3_3:-35452945 Msa0450130:CDS
GGCCAAGTTGAGAGAAGCTA+AGG 0.545213 3_3:+35452779 None:intergenic
GTTCATCCATGGCATTTGCA+TGG 0.550858 3_3:-35451535 Msa0450130:CDS
AGGTTTAAGGCAGACAACCC+TGG 0.556431 3_3:-35451382 Msa0450130:CDS
GTTCACGAAATTAGCTGCTA+GGG 0.560510 3_3:+35452908 None:intergenic
ATCACATTATGGCCTTTGGG+TGG 0.566948 3_3:-35451125 Msa0450130:three_prime_UTR
AATGGGAATCTATCTGGTGG+TGG 0.568086 3_3:+35451691 None:intergenic
CTATGCTATCAACCGTGAAT+GGG 0.572088 3_3:+35452124 None:intergenic
CTGCCTCAATGCAATTTCAA+AGG 0.573833 3_3:-35452879 Msa0450130:CDS
CATAAGGGAGTGATTGGTGG+TGG 0.576952 3_3:+35451937 None:intergenic
CTTGTTGTGGTGGAAGCAGA+TGG 0.578406 3_3:-35452159 Msa0450130:CDS
TTACACGTTTCACTTAAATG+AGG 0.579316 3_3:-35451611 Msa0450130:CDS
CATCTGCTTCCACCACAACA+AGG 0.580093 3_3:+35452160 None:intergenic
CCAAGTACAACAACGGGTAA+CGG 0.582426 3_3:-35451637 Msa0450130:CDS
ATATAGGGAAAGTTGAGGCA+GGG 0.586056 3_3:+35451962 None:intergenic
ATAACTGCAGTGTTCCTCAA+AGG 0.587942 3_3:+35451430 None:intergenic
CCACCTTTGAAATTGCATTG+AGG 0.597277 3_3:+35452876 None:intergenic
TTTGAAATTGCATTGAGGCA+GGG 0.606053 3_3:+35452881 None:intergenic
CCTCAATGCAATTTCAAAGG+TGG 0.612230 3_3:-35452876 Msa0450130:CDS
TCATGTCCATGCAAATGCCA+TGG 0.613062 3_3:+35451529 None:intergenic
CCTACACATGCTCTTACTTG+TGG 0.613192 3_3:-35451268 Msa0450130:CDS
GAATCACACCCTTGTTGTGG+TGG 0.615258 3_3:-35452169 Msa0450130:CDS
CCTTCATGAATAATATATAG+GGG 0.621956 3_3:-35451036 Msa0450130:three_prime_UTR
ATACTAGTGAAGTTCATCCA+TGG 0.627356 3_3:-35451546 Msa0450130:CDS
CTACTCAGTCCTTCTACACA+CGG 0.628943 3_3:-35452082 Msa0450130:CDS
AGTTAATCCAAGTACAACAA+CGG 0.640190 3_3:-35451644 Msa0450130:CDS
GATAGCATAGACATCTACTC+AGG 0.642343 3_3:-35452111 Msa0450130:CDS
GCTTGCTCATCAATGGAAGA+GGG 0.643075 3_3:-35452944 Msa0450130:CDS
ATCTGCTTCCACCACAACAA+GGG 0.647563 3_3:+35452161 None:intergenic
GTAGAATCACACCCTTGTTG+TGG 0.648768 3_3:-35452172 Msa0450130:intron
CGTGTGTAGAAGGACTGAGT+AGG 0.651575 3_3:+35452083 None:intergenic
TCAAATGGGAATCTATCTGG+TGG 0.659562 3_3:+35451688 None:intergenic
AACAAAGCAAAACCACCCAA+AGG 0.672305 3_3:+35451113 None:intergenic
TGGTAATATAGGGAAAGTTG+AGG 0.684103 3_3:+35451957 None:intergenic
CCACAAGTAAGAGCATGTGT+AGG 0.697143 3_3:+35451268 None:intergenic
AATGAAATGCCCAAACACCA+GGG 0.701200 3_3:+35451365 None:intergenic
GAAATTGCATTGAGGCAGGG+TGG 0.703546 3_3:+35452884 None:intergenic
TGTTGAGGAGGTAAATTGTG+CGG 0.723525 3_3:+35451833 None:intergenic
GGTGAAGGGAAATTTCAACC+AGG 0.727102 3_3:-35451487 Msa0450130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGTGTATAAAAAAATGTT+TGG - chr3_3:35451547-35451566 Msa0450130:CDS 15.0%
!! CATTACAAAATAATCTTAAA+CGG + chr3_3:35451615-35451634 None:intergenic 15.0%
!!! GTTTAAGATTATTTTGTAAT+GGG - chr3_3:35451614-35451633 Msa0450130:CDS 15.0%
!!! TTTAAGATTATTTTGTAATG+GGG - chr3_3:35451615-35451634 Msa0450130:CDS 15.0%
!! ATTGTGTATAAAAAAATGTT+TGG - chr3_3:35451547-35451566 Msa0450130:CDS 15.0%
!! CATTACAAAATAATCTTAAA+CGG + chr3_3:35451615-35451634 None:intergenic 15.0%
!!! GTTTAAGATTATTTTGTAAT+GGG - chr3_3:35451614-35451633 Msa0450130:CDS 15.0%
!!! TTTAAGATTATTTTGTAATG+GGG - chr3_3:35451615-35451634 Msa0450130:CDS 15.0%
!! AAAAAAGTCATCATAATGTA+TGG + chr3_3:35451120-35451139 None:intergenic 20.0%
!! AATGCAAATATATATCTAGT+AGG + chr3_3:35451369-35451388 None:intergenic 20.0%
!!! CGTTTAAGATTATTTTGTAA+TGG - chr3_3:35451613-35451632 Msa0450130:CDS 20.0%
!! CCAAATAAAGTATATATTTG+AGG + chr3_3:35451678-35451697 None:intergenic 20.0%
!! CCTCAAATATATACTTTATT+TGG - chr3_3:35451675-35451694 Msa0450130:CDS 20.0%
!! ATCATTCATTCATCAATTAA+CGG - chr3_3:35451870-35451889 Msa0450130:CDS 20.0%
!! ATTATAGTTAAGGATTGTTA+GGG + chr3_3:35452100-35452119 None:intergenic 20.0%
!! TATTATAGTTAAGGATTGTT+AGG + chr3_3:35452101-35452120 None:intergenic 20.0%
!! TAAATATGTCGAAATCAAAT+GGG + chr3_3:35452416-35452435 None:intergenic 20.0%
!! TTAAATATGTCGAAATCAAA+TGG + chr3_3:35452417-35452436 None:intergenic 20.0%
!!! TTTAAGTTATCATCACATTA+TGG - chr3_3:35452951-35452970 Msa0450130:intron 20.0%
!! AAAAAAGTCATCATAATGTA+TGG + chr3_3:35451120-35451139 None:intergenic 20.0%
!! AATGCAAATATATATCTAGT+AGG + chr3_3:35451369-35451388 None:intergenic 20.0%
!!! CGTTTAAGATTATTTTGTAA+TGG - chr3_3:35451613-35451632 Msa0450130:CDS 20.0%
!! CCAAATAAAGTATATATTTG+AGG + chr3_3:35451678-35451697 None:intergenic 20.0%
!! CCTCAAATATATACTTTATT+TGG - chr3_3:35451675-35451694 Msa0450130:CDS 20.0%
!! ATCATTCATTCATCAATTAA+CGG - chr3_3:35451870-35451889 Msa0450130:CDS 20.0%
!! ATTATAGTTAAGGATTGTTA+GGG + chr3_3:35452100-35452119 None:intergenic 20.0%
!! TATTATAGTTAAGGATTGTT+AGG + chr3_3:35452101-35452120 None:intergenic 20.0%
!! TAAATATGTCGAAATCAAAT+GGG + chr3_3:35452416-35452435 None:intergenic 20.0%
!! TTAAATATGTCGAAATCAAA+TGG + chr3_3:35452417-35452436 None:intergenic 20.0%
!!! TTTAAGTTATCATCACATTA+TGG - chr3_3:35452951-35452970 Msa0450130:intron 20.0%
!! GAAAAAAGACAATGTTGTTT+AGG + chr3_3:35451050-35451069 None:intergenic 25.0%
! GGAAAAAAATGTCATTCATA+AGG + chr3_3:35451090-35451109 None:intergenic 25.0%
!!! CTAATTCTGTAGGTTTTTTT+TGG + chr3_3:35451271-35451290 None:intergenic 25.0%
! CAAAAAAAACCTACAGAATT+AGG - chr3_3:35451269-35451288 Msa0450130:CDS 25.0%
! AGATCAAAAACTTACTGAAA+TGG + chr3_3:35451332-35451351 None:intergenic 25.0%
!!! CATTTTTTTATACACAATCG+AGG + chr3_3:35451545-35451564 None:intergenic 25.0%
! TTCATCGATAAAATCAGTTT+AGG + chr3_3:35451822-35451841 None:intergenic 25.0%
! TCTACATGACACAAAAATTA+TGG + chr3_3:35451900-35451919 None:intergenic 25.0%
! AAAACAAATCATTTCCAGAA+TGG - chr3_3:35452203-35452222 Msa0450130:intron 25.0%
! AAACAAATCATTTCCAGAAT+GGG - chr3_3:35452204-35452223 Msa0450130:intron 25.0%
! AAATCGGTAATTTCACTAAA+TGG - chr3_3:35452286-35452305 Msa0450130:intron 25.0%
! AATCGGTAATTTCACTAAAT+GGG - chr3_3:35452287-35452306 Msa0450130:intron 25.0%
! AAAGAAAGAAAAACACTCTT+AGG + chr3_3:35452919-35452938 None:intergenic 25.0%
!!! ATTGTTTCTCTTTTATCATC+AGG - chr3_3:35453012-35453031 Msa0450130:intron 25.0%
! GCCTTCATGAATAATATATA+GGG - chr3_3:35453050-35453069 Msa0450130:intron 25.0%
! CCTTCATGAATAATATATAG+GGG - chr3_3:35453051-35453070 Msa0450130:intron 25.0%
!! GAAAAAAGACAATGTTGTTT+AGG + chr3_3:35451050-35451069 None:intergenic 25.0%
! GGAAAAAAATGTCATTCATA+AGG + chr3_3:35451090-35451109 None:intergenic 25.0%
!!! CTAATTCTGTAGGTTTTTTT+TGG + chr3_3:35451271-35451290 None:intergenic 25.0%
! CAAAAAAAACCTACAGAATT+AGG - chr3_3:35451269-35451288 Msa0450130:CDS 25.0%
! AGATCAAAAACTTACTGAAA+TGG + chr3_3:35451332-35451351 None:intergenic 25.0%
!!! CATTTTTTTATACACAATCG+AGG + chr3_3:35451545-35451564 None:intergenic 25.0%
! TTCATCGATAAAATCAGTTT+AGG + chr3_3:35451822-35451841 None:intergenic 25.0%
! TCTACATGACACAAAAATTA+TGG + chr3_3:35451900-35451919 None:intergenic 25.0%
! AAAACAAATCATTTCCAGAA+TGG - chr3_3:35452203-35452222 Msa0450130:intron 25.0%
! AAACAAATCATTTCCAGAAT+GGG - chr3_3:35452204-35452223 Msa0450130:intron 25.0%
! AAATCGGTAATTTCACTAAA+TGG - chr3_3:35452286-35452305 Msa0450130:intron 25.0%
! AATCGGTAATTTCACTAAAT+GGG - chr3_3:35452287-35452306 Msa0450130:intron 25.0%
! AAAGAAAGAAAAACACTCTT+AGG + chr3_3:35452919-35452938 None:intergenic 25.0%
!!! ATTGTTTCTCTTTTATCATC+AGG - chr3_3:35453012-35453031 Msa0450130:intron 25.0%
! GCCTTCATGAATAATATATA+GGG - chr3_3:35453050-35453069 Msa0450130:intron 25.0%
! CCTTCATGAATAATATATAG+GGG - chr3_3:35453051-35453070 Msa0450130:intron 25.0%
AAGTCATCATAATGTATGGT+GGG + chr3_3:35451116-35451135 None:intergenic 30.0%
AAAGTCATCATAATGTATGG+TGG + chr3_3:35451117-35451136 None:intergenic 30.0%
!! AAGATTATTTTGTAATGGGG+AGG - chr3_3:35451618-35451637 Msa0450130:CDS 30.0%
CAGGGTTTGTATTATAGTTA+AGG + chr3_3:35452110-35452129 None:intergenic 30.0%
TATTTGATTGCTCCTAAGTA+AGG + chr3_3:35452348-35452367 None:intergenic 30.0%
AAATCAAATGGGAATCTATC+TGG + chr3_3:35452405-35452424 None:intergenic 30.0%
AGTTAATCCAAGTACAACAA+CGG - chr3_3:35452443-35452462 Msa0450130:intron 30.0%
TTACACGTTTCACTTAAATG+AGG - chr3_3:35452476-35452495 Msa0450130:intron 30.0%
! ATTTGCATGGACATGATTTT+TGG - chr3_3:35452565-35452584 Msa0450130:intron 30.0%
! TTTGCATGGACATGATTTTT+GGG - chr3_3:35452566-35452585 Msa0450130:intron 30.0%
ACTGCAGTTATATTTCCTTA+TGG - chr3_3:35452669-35452688 Msa0450130:intron 30.0%
CAGTTATATTTCCTTATGGT+TGG - chr3_3:35452673-35452692 Msa0450130:intron 30.0%
AAAACACTCTTAGGAGATTA+AGG + chr3_3:35452910-35452929 None:intergenic 30.0%
CGCCTTCATGAATAATATAT+AGG - chr3_3:35453049-35453068 Msa0450130:intron 30.0%
CCCCTATATATTATTCATGA+AGG + chr3_3:35453054-35453073 None:intergenic 30.0%
AAGTCATCATAATGTATGGT+GGG + chr3_3:35451116-35451135 None:intergenic 30.0%
AAAGTCATCATAATGTATGG+TGG + chr3_3:35451117-35451136 None:intergenic 30.0%
!! AAGATTATTTTGTAATGGGG+AGG - chr3_3:35451618-35451637 Msa0450130:CDS 30.0%
CAGGGTTTGTATTATAGTTA+AGG + chr3_3:35452110-35452129 None:intergenic 30.0%
TATTTGATTGCTCCTAAGTA+AGG + chr3_3:35452348-35452367 None:intergenic 30.0%
AAATCAAATGGGAATCTATC+TGG + chr3_3:35452405-35452424 None:intergenic 30.0%
AGTTAATCCAAGTACAACAA+CGG - chr3_3:35452443-35452462 Msa0450130:intron 30.0%
TTACACGTTTCACTTAAATG+AGG - chr3_3:35452476-35452495 Msa0450130:intron 30.0%
! ATTTGCATGGACATGATTTT+TGG - chr3_3:35452565-35452584 Msa0450130:intron 30.0%
! TTTGCATGGACATGATTTTT+GGG - chr3_3:35452566-35452585 Msa0450130:intron 30.0%
ACTGCAGTTATATTTCCTTA+TGG - chr3_3:35452669-35452688 Msa0450130:intron 30.0%
CAGTTATATTTCCTTATGGT+TGG - chr3_3:35452673-35452692 Msa0450130:intron 30.0%
AAAACACTCTTAGGAGATTA+AGG + chr3_3:35452910-35452929 None:intergenic 30.0%
CGCCTTCATGAATAATATAT+AGG - chr3_3:35453049-35453068 Msa0450130:intron 30.0%
CCCCTATATATTATTCATGA+AGG + chr3_3:35453054-35453073 None:intergenic 30.0%
!! ATCATAATGTATGGTGGGTT+AGG + chr3_3:35451111-35451130 None:intergenic 35.0%
!!! TTTTAGAGCTTGCTCATCAA+TGG - chr3_3:35451136-35451155 Msa0450130:three_prime_UTR 35.0%
! TTTGAAATTGCATTGAGGCA+GGG + chr3_3:35451209-35451228 None:intergenic 35.0%
ACTTGCAATCCTAATTCTGT+AGG + chr3_3:35451281-35451300 None:intergenic 35.0%
ATCTCTCTTTCTTGTGTAGT+AGG - chr3_3:35451397-35451416 Msa0450130:CDS 35.0%
TCTTGTGTAGTAGGTATGAA+AGG - chr3_3:35451406-35451425 Msa0450130:CDS 35.0%
GCAAGGAAAACATGACTAAT+AGG - chr3_3:35451475-35451494 Msa0450130:CDS 35.0%
TTGGTAGATGTGCAAATCTA+TGG - chr3_3:35451566-35451585 Msa0450130:CDS 35.0%
TGGTAATATAGGGAAAGTTG+AGG + chr3_3:35452133-35452152 None:intergenic 35.0%
!! TGATTGGTGGTGGTAATATA+GGG + chr3_3:35452143-35452162 None:intergenic 35.0%
CTCAACACACAAAGCAAAAT+CGG - chr3_3:35452270-35452289 Msa0450130:intron 35.0%
!! AGGTGTTATTGGCAATGTTA+AGG + chr3_3:35452328-35452347 None:intergenic 35.0%
TTGCCAATAACACCTTACTT+AGG - chr3_3:35452333-35452352 Msa0450130:intron 35.0%
GTTGTTGTACTTGGATTAAC+TGG + chr3_3:35452444-35452463 None:intergenic 35.0%
GTTAATCCAAGTACAACAAC+GGG - chr3_3:35452444-35452463 Msa0450130:intron 35.0%
ATACTAGTGAAGTTCATCCA+TGG - chr3_3:35452541-35452560 Msa0450130:intron 35.0%
!!! TGGACATGATTTTTGGGTTT+TGG - chr3_3:35452572-35452591 Msa0450130:intron 35.0%
!!! GGACATGATTTTTGGGTTTT+GGG - chr3_3:35452573-35452592 Msa0450130:intron 35.0%
!!! GATTTTTGGGTTTTGGGATA+TGG - chr3_3:35452579-35452598 Msa0450130:intron 35.0%
! AAACTTTTCTGATGAACACC+TGG + chr3_3:35452621-35452640 None:intergenic 35.0%
TTGGACTGCATTAAGGTTTA+AGG - chr3_3:35452692-35452711 Msa0450130:intron 35.0%
ATTGAACCACATTTGCATCT+GGG - chr3_3:35452750-35452769 Msa0450130:intron 35.0%
!!! AATGGGTGTTATTTTTGCTG+AGG - chr3_3:35452773-35452792 Msa0450130:CDS 35.0%
!!! ATGGGTGTTATTTTTGCTGA+GGG - chr3_3:35452774-35452793 Msa0450130:CDS 35.0%
!! CTGAGGGTGTTCATAAAATT+GGG - chr3_3:35452790-35452809 Msa0450130:CDS 35.0%
ACTCTTAGGAGATTAAGGTT+TGG + chr3_3:35452905-35452924 None:intergenic 35.0%
TATCATCACATTATGGCCTT+TGG - chr3_3:35452958-35452977 Msa0450130:intron 35.0%
ATCATCACATTATGGCCTTT+GGG - chr3_3:35452959-35452978 Msa0450130:intron 35.0%
TATTATTCATGAAGGCGAGT+TGG + chr3_3:35453046-35453065 None:intergenic 35.0%
!! ATCATAATGTATGGTGGGTT+AGG + chr3_3:35451111-35451130 None:intergenic 35.0%
!!! TTTTAGAGCTTGCTCATCAA+TGG - chr3_3:35451136-35451155 Msa0450130:three_prime_UTR 35.0%
! TTTGAAATTGCATTGAGGCA+GGG + chr3_3:35451209-35451228 None:intergenic 35.0%
ACTTGCAATCCTAATTCTGT+AGG + chr3_3:35451281-35451300 None:intergenic 35.0%
ATCTCTCTTTCTTGTGTAGT+AGG - chr3_3:35451397-35451416 Msa0450130:CDS 35.0%
TCTTGTGTAGTAGGTATGAA+AGG - chr3_3:35451406-35451425 Msa0450130:CDS 35.0%
GCAAGGAAAACATGACTAAT+AGG - chr3_3:35451475-35451494 Msa0450130:CDS 35.0%
TTGGTAGATGTGCAAATCTA+TGG - chr3_3:35451566-35451585 Msa0450130:CDS 35.0%
TGGTAATATAGGGAAAGTTG+AGG + chr3_3:35452133-35452152 None:intergenic 35.0%
!! TGATTGGTGGTGGTAATATA+GGG + chr3_3:35452143-35452162 None:intergenic 35.0%
CTCAACACACAAAGCAAAAT+CGG - chr3_3:35452270-35452289 Msa0450130:intron 35.0%
!! AGGTGTTATTGGCAATGTTA+AGG + chr3_3:35452328-35452347 None:intergenic 35.0%
TTGCCAATAACACCTTACTT+AGG - chr3_3:35452333-35452352 Msa0450130:intron 35.0%
GTTGTTGTACTTGGATTAAC+TGG + chr3_3:35452444-35452463 None:intergenic 35.0%
GTTAATCCAAGTACAACAAC+GGG - chr3_3:35452444-35452463 Msa0450130:intron 35.0%
ATACTAGTGAAGTTCATCCA+TGG - chr3_3:35452541-35452560 Msa0450130:intron 35.0%
!!! TGGACATGATTTTTGGGTTT+TGG - chr3_3:35452572-35452591 Msa0450130:intron 35.0%
!!! GGACATGATTTTTGGGTTTT+GGG - chr3_3:35452573-35452592 Msa0450130:intron 35.0%
!!! GATTTTTGGGTTTTGGGATA+TGG - chr3_3:35452579-35452598 Msa0450130:intron 35.0%
! AAACTTTTCTGATGAACACC+TGG + chr3_3:35452621-35452640 None:intergenic 35.0%
TTGGACTGCATTAAGGTTTA+AGG - chr3_3:35452692-35452711 Msa0450130:intron 35.0%
ATTGAACCACATTTGCATCT+GGG - chr3_3:35452750-35452769 Msa0450130:intron 35.0%
!!! AATGGGTGTTATTTTTGCTG+AGG - chr3_3:35452773-35452792 Msa0450130:CDS 35.0%
!!! ATGGGTGTTATTTTTGCTGA+GGG - chr3_3:35452774-35452793 Msa0450130:CDS 35.0%
!! CTGAGGGTGTTCATAAAATT+GGG - chr3_3:35452790-35452809 Msa0450130:CDS 35.0%
ACTCTTAGGAGATTAAGGTT+TGG + chr3_3:35452905-35452924 None:intergenic 35.0%
TATCATCACATTATGGCCTT+TGG - chr3_3:35452958-35452977 Msa0450130:intron 35.0%
ATCATCACATTATGGCCTTT+GGG - chr3_3:35452959-35452978 Msa0450130:intron 35.0%
TATTATTCATGAAGGCGAGT+TGG + chr3_3:35453046-35453065 None:intergenic 35.0%
GTTCACGAAATTAGCTGCTA+GGG + chr3_3:35451182-35451201 None:intergenic 40.0%
TGTTCACGAAATTAGCTGCT+AGG + chr3_3:35451183-35451202 None:intergenic 40.0%
!! CTTTGAAATTGCATTGAGGC+AGG + chr3_3:35451210-35451229 None:intergenic 40.0%
CTGCCTCAATGCAATTTCAA+AGG - chr3_3:35451208-35451227 Msa0450130:three_prime_UTR 40.0%
!! CCACCTTTGAAATTGCATTG+AGG + chr3_3:35451214-35451233 None:intergenic 40.0%
CCTCAATGCAATTTCAAAGG+TGG - chr3_3:35451211-35451230 Msa0450130:exon 40.0%
ATTATTCCATTCGCATCGCA+AGG - chr3_3:35451458-35451477 Msa0450130:CDS 40.0%
!! TATTTTGTAATGGGGAGGCT+TGG - chr3_3:35451623-35451642 Msa0450130:CDS 40.0%
CTATGCTATCAACCGTGAAT+GGG + chr3_3:35451966-35451985 None:intergenic 40.0%
TCTATGCTATCAACCGTGAA+TGG + chr3_3:35451967-35451986 None:intergenic 40.0%
GATAGCATAGACATCTACTC+AGG - chr3_3:35451976-35451995 Msa0450130:CDS 40.0%
!!! AGTAGTTTTGGTTGGGATCT+TGG + chr3_3:35452032-35452051 None:intergenic 40.0%
AAGATCCCAACCAAAACTAC+TGG - chr3_3:35452031-35452050 Msa0450130:CDS 40.0%
!! CAAAACTACTGGCTCTCAAT+CGG - chr3_3:35452042-35452061 Msa0450130:CDS 40.0%
TTAAGGATTGTTAGGGCTTG+TGG + chr3_3:35452093-35452112 None:intergenic 40.0%
ATATAGGGAAAGTTGAGGCA+GGG + chr3_3:35452128-35452147 None:intergenic 40.0%
AATATAGGGAAAGTTGAGGC+AGG + chr3_3:35452129-35452148 None:intergenic 40.0%
!! GTGATTGGTGGTGGTAATAT+AGG + chr3_3:35452144-35452163 None:intergenic 40.0%
TGTTGAGGAGGTAAATTGTG+CGG + chr3_3:35452257-35452276 None:intergenic 40.0%
! TTTTGCTTTGTGTGTTGAGG+AGG + chr3_3:35452269-35452288 None:intergenic 40.0%
! CGATTTTGCTTTGTGTGTTG+AGG + chr3_3:35452272-35452291 None:intergenic 40.0%
! GCTCCTAAGTAAGGTGTTAT+TGG + chr3_3:35452339-35452358 None:intergenic 40.0%
TCAAATGGGAATCTATCTGG+TGG + chr3_3:35452402-35452421 None:intergenic 40.0%
ATAACTGCAGTGTTCCTCAA+AGG + chr3_3:35452660-35452679 None:intergenic 40.0%
CTTAATGCAGTCCAACCATA+AGG + chr3_3:35452687-35452706 None:intergenic 40.0%
CTTATGGTTGGACTGCATTA+AGG - chr3_3:35452685-35452704 Msa0450130:intron 40.0%
AATGAAATGCCCAAACACCA+GGG + chr3_3:35452725-35452744 None:intergenic 40.0%
CATTGAACCACATTTGCATC+TGG - chr3_3:35452749-35452768 Msa0450130:intron 40.0%
!! GCTGAGGGTGTTCATAAAAT+TGG - chr3_3:35452789-35452808 Msa0450130:CDS 40.0%
!! GGTGTTGTTTAGGAACATCT+TGG + chr3_3:35452853-35452872 None:intergenic 40.0%
AACAAAGCAAAACCACCCAA+AGG + chr3_3:35452977-35452996 None:intergenic 40.0%
GTTCACGAAATTAGCTGCTA+GGG + chr3_3:35451182-35451201 None:intergenic 40.0%
TGTTCACGAAATTAGCTGCT+AGG + chr3_3:35451183-35451202 None:intergenic 40.0%
!! CTTTGAAATTGCATTGAGGC+AGG + chr3_3:35451210-35451229 None:intergenic 40.0%
CTGCCTCAATGCAATTTCAA+AGG - chr3_3:35451208-35451227 Msa0450130:three_prime_UTR 40.0%
!! CCACCTTTGAAATTGCATTG+AGG + chr3_3:35451214-35451233 None:intergenic 40.0%
CCTCAATGCAATTTCAAAGG+TGG - chr3_3:35451211-35451230 Msa0450130:exon 40.0%
ATTATTCCATTCGCATCGCA+AGG - chr3_3:35451458-35451477 Msa0450130:CDS 40.0%
!! TATTTTGTAATGGGGAGGCT+TGG - chr3_3:35451623-35451642 Msa0450130:CDS 40.0%
CTATGCTATCAACCGTGAAT+GGG + chr3_3:35451966-35451985 None:intergenic 40.0%
TCTATGCTATCAACCGTGAA+TGG + chr3_3:35451967-35451986 None:intergenic 40.0%
GATAGCATAGACATCTACTC+AGG - chr3_3:35451976-35451995 Msa0450130:CDS 40.0%
!!! AGTAGTTTTGGTTGGGATCT+TGG + chr3_3:35452032-35452051 None:intergenic 40.0%
AAGATCCCAACCAAAACTAC+TGG - chr3_3:35452031-35452050 Msa0450130:CDS 40.0%
!! CAAAACTACTGGCTCTCAAT+CGG - chr3_3:35452042-35452061 Msa0450130:CDS 40.0%
TTAAGGATTGTTAGGGCTTG+TGG + chr3_3:35452093-35452112 None:intergenic 40.0%
ATATAGGGAAAGTTGAGGCA+GGG + chr3_3:35452128-35452147 None:intergenic 40.0%
AATATAGGGAAAGTTGAGGC+AGG + chr3_3:35452129-35452148 None:intergenic 40.0%
!! GTGATTGGTGGTGGTAATAT+AGG + chr3_3:35452144-35452163 None:intergenic 40.0%
TGTTGAGGAGGTAAATTGTG+CGG + chr3_3:35452257-35452276 None:intergenic 40.0%
! TTTTGCTTTGTGTGTTGAGG+AGG + chr3_3:35452269-35452288 None:intergenic 40.0%
! CGATTTTGCTTTGTGTGTTG+AGG + chr3_3:35452272-35452291 None:intergenic 40.0%
! GCTCCTAAGTAAGGTGTTAT+TGG + chr3_3:35452339-35452358 None:intergenic 40.0%
TCAAATGGGAATCTATCTGG+TGG + chr3_3:35452402-35452421 None:intergenic 40.0%
ATAACTGCAGTGTTCCTCAA+AGG + chr3_3:35452660-35452679 None:intergenic 40.0%
CTTAATGCAGTCCAACCATA+AGG + chr3_3:35452687-35452706 None:intergenic 40.0%
CTTATGGTTGGACTGCATTA+AGG - chr3_3:35452685-35452704 Msa0450130:intron 40.0%
AATGAAATGCCCAAACACCA+GGG + chr3_3:35452725-35452744 None:intergenic 40.0%
CATTGAACCACATTTGCATC+TGG - chr3_3:35452749-35452768 Msa0450130:intron 40.0%
!! GCTGAGGGTGTTCATAAAAT+TGG - chr3_3:35452789-35452808 Msa0450130:CDS 40.0%
!! GGTGTTGTTTAGGAACATCT+TGG + chr3_3:35452853-35452872 None:intergenic 40.0%
AACAAAGCAAAACCACCCAA+AGG + chr3_3:35452977-35452996 None:intergenic 40.0%
! AGCTTGCTCATCAATGGAAG+AGG - chr3_3:35451142-35451161 Msa0450130:three_prime_UTR 45.0%
GCTTGCTCATCAATGGAAGA+GGG - chr3_3:35451143-35451162 Msa0450130:three_prime_UTR 45.0%
GTCCTTAGCTTCTCTCAACT+TGG - chr3_3:35451306-35451325 Msa0450130:CDS 45.0%
! TGTTTTCCTTGCGATGCGAA+TGG + chr3_3:35451467-35451486 None:intergenic 45.0%
! TCTTAAACGGCTAGTTGAGC+TGG + chr3_3:35451602-35451621 None:intergenic 45.0%
GTAGAATCACACCCTTGTTG+TGG - chr3_3:35451915-35451934 Msa0450130:CDS 45.0%
ATCTGCTTCCACCACAACAA+GGG + chr3_3:35451929-35451948 None:intergenic 45.0%
AAACTACGTGCACCCATTCA+CGG - chr3_3:35451951-35451970 Msa0450130:CDS 45.0%
CTACTCAGTCCTTCTACACA+CGG - chr3_3:35452005-35452024 Msa0450130:CDS 45.0%
!!! GAGAGCCAGTAGTTTTGGTT+GGG + chr3_3:35452039-35452058 None:intergenic 45.0%
!!! TGAGAGCCAGTAGTTTTGGT+TGG + chr3_3:35452040-35452059 None:intergenic 45.0%
! CGATTGAGAGCCAGTAGTTT+TGG + chr3_3:35452044-35452063 None:intergenic 45.0%
TTCCATAAGGGAGTGATTGG+TGG + chr3_3:35452156-35452175 None:intergenic 45.0%
TCGTTCCATAAGGGAGTGAT+TGG + chr3_3:35452159-35452178 None:intergenic 45.0%
CGCTCGAAATCGTTCCATAA+GGG + chr3_3:35452168-35452187 None:intergenic 45.0%
!!! TCTGCGATTTTTGGTGTCTG+TGG + chr3_3:35452234-35452253 None:intergenic 45.0%
! ATTGTGCGGTCTGCGATTTT+TGG + chr3_3:35452243-35452262 None:intergenic 45.0%
AATGGGAATCTATCTGGTGG+TGG + chr3_3:35452399-35452418 None:intergenic 45.0%
CCAAGTACAACAACGGGTAA+CGG - chr3_3:35452450-35452469 Msa0450130:intron 45.0%
GTTCATCCATGGCATTTGCA+TGG - chr3_3:35452552-35452571 Msa0450130:intron 45.0%
TCATGTCCATGCAAATGCCA+TGG + chr3_3:35452561-35452580 None:intergenic 45.0%
!!! TGGGTTTTGGGATATGGTGA+AGG - chr3_3:35452585-35452604 Msa0450130:intron 45.0%
!!! GGGTTTTGGGATATGGTGAA+GGG - chr3_3:35452586-35452605 Msa0450130:intron 45.0%
GGTGAAGGGAAATTTCAACC+AGG - chr3_3:35452600-35452619 Msa0450130:intron 45.0%
ATTTGACACGTGCACCTTTG+AGG - chr3_3:35452643-35452662 Msa0450130:intron 45.0%
CAATGAAATGCCCAAACACC+AGG + chr3_3:35452726-35452745 None:intergenic 45.0%
ACCACATTTGCATCTGGGAA+TGG - chr3_3:35452755-35452774 Msa0450130:intron 45.0%
CCACATTTGCATCTGGGAAT+GGG - chr3_3:35452756-35452775 Msa0450130:intron 45.0%
! CCACAAGTAAGAGCATGTGT+AGG + chr3_3:35452822-35452841 None:intergenic 45.0%
CCTACACATGCTCTTACTTG+TGG - chr3_3:35452819-35452838 Msa0450130:CDS 45.0%
!! CTCAGTTGTGGGTGTTGTTT+AGG + chr3_3:35452863-35452882 None:intergenic 45.0%
! GCTTAGCTAGTCTCAGTTGT+GGG + chr3_3:35452874-35452893 None:intergenic 45.0%
! AGCTTAGCTAGTCTCAGTTG+TGG + chr3_3:35452875-35452894 None:intergenic 45.0%
ATCACATTATGGCCTTTGGG+TGG - chr3_3:35452962-35452981 Msa0450130:intron 45.0%
! ATGAAGGCGAGTTGGACAAA+TGG + chr3_3:35453038-35453057 None:intergenic 45.0%
! AGCTTGCTCATCAATGGAAG+AGG - chr3_3:35451142-35451161 Msa0450130:three_prime_UTR 45.0%
GCTTGCTCATCAATGGAAGA+GGG - chr3_3:35451143-35451162 Msa0450130:three_prime_UTR 45.0%
GTCCTTAGCTTCTCTCAACT+TGG - chr3_3:35451306-35451325 Msa0450130:CDS 45.0%
! TGTTTTCCTTGCGATGCGAA+TGG + chr3_3:35451467-35451486 None:intergenic 45.0%
! TCTTAAACGGCTAGTTGAGC+TGG + chr3_3:35451602-35451621 None:intergenic 45.0%
GTAGAATCACACCCTTGTTG+TGG - chr3_3:35451915-35451934 Msa0450130:CDS 45.0%
ATCTGCTTCCACCACAACAA+GGG + chr3_3:35451929-35451948 None:intergenic 45.0%
AAACTACGTGCACCCATTCA+CGG - chr3_3:35451951-35451970 Msa0450130:CDS 45.0%
CTACTCAGTCCTTCTACACA+CGG - chr3_3:35452005-35452024 Msa0450130:CDS 45.0%
!!! GAGAGCCAGTAGTTTTGGTT+GGG + chr3_3:35452039-35452058 None:intergenic 45.0%
!!! TGAGAGCCAGTAGTTTTGGT+TGG + chr3_3:35452040-35452059 None:intergenic 45.0%
! CGATTGAGAGCCAGTAGTTT+TGG + chr3_3:35452044-35452063 None:intergenic 45.0%
TTCCATAAGGGAGTGATTGG+TGG + chr3_3:35452156-35452175 None:intergenic 45.0%
TCGTTCCATAAGGGAGTGAT+TGG + chr3_3:35452159-35452178 None:intergenic 45.0%
CGCTCGAAATCGTTCCATAA+GGG + chr3_3:35452168-35452187 None:intergenic 45.0%
!!! TCTGCGATTTTTGGTGTCTG+TGG + chr3_3:35452234-35452253 None:intergenic 45.0%
! ATTGTGCGGTCTGCGATTTT+TGG + chr3_3:35452243-35452262 None:intergenic 45.0%
AATGGGAATCTATCTGGTGG+TGG + chr3_3:35452399-35452418 None:intergenic 45.0%
CCAAGTACAACAACGGGTAA+CGG - chr3_3:35452450-35452469 Msa0450130:intron 45.0%
GTTCATCCATGGCATTTGCA+TGG - chr3_3:35452552-35452571 Msa0450130:intron 45.0%
TCATGTCCATGCAAATGCCA+TGG + chr3_3:35452561-35452580 None:intergenic 45.0%
!!! TGGGTTTTGGGATATGGTGA+AGG - chr3_3:35452585-35452604 Msa0450130:intron 45.0%
!!! GGGTTTTGGGATATGGTGAA+GGG - chr3_3:35452586-35452605 Msa0450130:intron 45.0%
GGTGAAGGGAAATTTCAACC+AGG - chr3_3:35452600-35452619 Msa0450130:intron 45.0%
ATTTGACACGTGCACCTTTG+AGG - chr3_3:35452643-35452662 Msa0450130:intron 45.0%
CAATGAAATGCCCAAACACC+AGG + chr3_3:35452726-35452745 None:intergenic 45.0%
ACCACATTTGCATCTGGGAA+TGG - chr3_3:35452755-35452774 Msa0450130:intron 45.0%
CCACATTTGCATCTGGGAAT+GGG - chr3_3:35452756-35452775 Msa0450130:intron 45.0%
! CCACAAGTAAGAGCATGTGT+AGG + chr3_3:35452822-35452841 None:intergenic 45.0%
CCTACACATGCTCTTACTTG+TGG - chr3_3:35452819-35452838 Msa0450130:CDS 45.0%
!! CTCAGTTGTGGGTGTTGTTT+AGG + chr3_3:35452863-35452882 None:intergenic 45.0%
! GCTTAGCTAGTCTCAGTTGT+GGG + chr3_3:35452874-35452893 None:intergenic 45.0%
! AGCTTAGCTAGTCTCAGTTG+TGG + chr3_3:35452875-35452894 None:intergenic 45.0%
ATCACATTATGGCCTTTGGG+TGG - chr3_3:35452962-35452981 Msa0450130:intron 45.0%
! ATGAAGGCGAGTTGGACAAA+TGG + chr3_3:35453038-35453057 None:intergenic 45.0%
GAAATTGCATTGAGGCAGGG+TGG + chr3_3:35451206-35451225 None:intergenic 50.0%
GGCGCTACTTCGAGAATCTT+GGG + chr3_3:35451250-35451269 None:intergenic 50.0%
TGGCGCTACTTCGAGAATCT+TGG + chr3_3:35451251-35451270 None:intergenic 50.0%
! GGCCAAGTTGAGAGAAGCTA+AGG + chr3_3:35451311-35451330 None:intergenic 50.0%
TGCAAATCTATGGCGTCGCA+TGG - chr3_3:35451576-35451595 Msa0450130:CDS 50.0%
! GAATCACACCCTTGTTGTGG+TGG - chr3_3:35451918-35451937 Msa0450130:CDS 50.0%
CATCTGCTTCCACCACAACA+AGG + chr3_3:35451930-35451949 None:intergenic 50.0%
! CTTGTTGTGGTGGAAGCAGA+TGG - chr3_3:35451928-35451947 Msa0450130:CDS 50.0%
CGTGTGTAGAAGGACTGAGT+AGG + chr3_3:35452007-35452026 None:intergenic 50.0%
GATCTTGGTCCGTGTGTAGA+AGG + chr3_3:35452017-35452036 None:intergenic 50.0%
CATAAGGGAGTGATTGGTGG+TGG + chr3_3:35452153-35452172 None:intergenic 50.0%
CACCACCAATCACTCCCTTA+TGG - chr3_3:35452151-35452170 Msa0450130:CDS 50.0%
GCGCTCGAAATCGTTCCATA+AGG + chr3_3:35452169-35452188 None:intergenic 50.0%
TGTCTGTGGTGTTCCCATTC+TGG + chr3_3:35452220-35452239 None:intergenic 50.0%
CCGTTACCCGTTGTTGTACT+TGG + chr3_3:35452453-35452472 None:intergenic 50.0%
! AGGTTTAAGGCAGACAACCC+TGG - chr3_3:35452705-35452724 Msa0450130:intron 50.0%
CCCATTCCCAGATGCAAATG+TGG + chr3_3:35452759-35452778 None:intergenic 50.0%
GAAATTGCATTGAGGCAGGG+TGG + chr3_3:35451206-35451225 None:intergenic 50.0%
GGCGCTACTTCGAGAATCTT+GGG + chr3_3:35451250-35451269 None:intergenic 50.0%
TGGCGCTACTTCGAGAATCT+TGG + chr3_3:35451251-35451270 None:intergenic 50.0%
! GGCCAAGTTGAGAGAAGCTA+AGG + chr3_3:35451311-35451330 None:intergenic 50.0%
TGCAAATCTATGGCGTCGCA+TGG - chr3_3:35451576-35451595 Msa0450130:CDS 50.0%
! GAATCACACCCTTGTTGTGG+TGG - chr3_3:35451918-35451937 Msa0450130:CDS 50.0%
CATCTGCTTCCACCACAACA+AGG + chr3_3:35451930-35451949 None:intergenic 50.0%
! CTTGTTGTGGTGGAAGCAGA+TGG - chr3_3:35451928-35451947 Msa0450130:CDS 50.0%
CGTGTGTAGAAGGACTGAGT+AGG + chr3_3:35452007-35452026 None:intergenic 50.0%
GATCTTGGTCCGTGTGTAGA+AGG + chr3_3:35452017-35452036 None:intergenic 50.0%
CATAAGGGAGTGATTGGTGG+TGG + chr3_3:35452153-35452172 None:intergenic 50.0%
CACCACCAATCACTCCCTTA+TGG - chr3_3:35452151-35452170 Msa0450130:CDS 50.0%
GCGCTCGAAATCGTTCCATA+AGG + chr3_3:35452169-35452188 None:intergenic 50.0%
TGTCTGTGGTGTTCCCATTC+TGG + chr3_3:35452220-35452239 None:intergenic 50.0%
CCGTTACCCGTTGTTGTACT+TGG + chr3_3:35452453-35452472 None:intergenic 50.0%
! AGGTTTAAGGCAGACAACCC+TGG - chr3_3:35452705-35452724 Msa0450130:intron 50.0%
CCCATTCCCAGATGCAAATG+TGG + chr3_3:35452759-35452778 None:intergenic 50.0%
AGGCAGACAACCCTGGTGTT+TGG - chr3_3:35452712-35452731 Msa0450130:intron 55.0%
GGCAGACAACCCTGGTGTTT+GGG - chr3_3:35452713-35452732 Msa0450130:intron 55.0%
AGGCAGACAACCCTGGTGTT+TGG - chr3_3:35452712-35452731 Msa0450130:intron 55.0%
GGCAGACAACCCTGGTGTTT+GGG - chr3_3:35452713-35452732 Msa0450130:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 35451035 35453074 35451035 ID=Msa0450130;Name=Msa0450130
chr3_3 mRNA 35451035 35453074 35451035 ID=Msa0450130-mRNA-1;Parent=Msa0450130;Name=Msa0450130-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0.5|0.33|0.66|1|1|3|193|388
chr3_3 exon 35451035 35452190 35451035 ID=Msa0450130-mRNA-1:exon:6286;Parent=Msa0450130-mRNA-1
chr3_3 exon 35452773 35452967 35452773 ID=Msa0450130-mRNA-1:exon:6285;Parent=Msa0450130-mRNA-1
chr3_3 exon 35453066 35453074 35453066 ID=Msa0450130-mRNA-1:exon:6284;Parent=Msa0450130-mRNA-1
chr3_3 CDS 35453066 35453074 35453066 ID=Msa0450130-mRNA-1:cds;Parent=Msa0450130-mRNA-1
chr3_3 CDS 35452773 35452967 35452773 ID=Msa0450130-mRNA-1:cds;Parent=Msa0450130-mRNA-1
chr3_3 CDS 35451228 35452190 35451228 ID=Msa0450130-mRNA-1:cds;Parent=Msa0450130-mRNA-1
chr3_3 three_prime_UTR 35451035 35451227 35451035 ID=Msa0450130-mRNA-1:three_prime_utr;Parent=Msa0450130-mRNA-1
Gene Sequence

>Msa0450130

ATGGATATGAGCTTGCTCATCAATGGAAGAGGGCAGTTCAATTGTTCCCTAGCAGCTAATTTCGTGAACACCACCCTGCCTCAATGCAATTTCAAAGGTGGTGAAGAATGCGCACCCAAGATTCTCGAAGTAGCGCCAAAAAAAACCTACAGAATTAGGATTGCAAGTACAACGTCCTTAGCTTCTCTCAACTTGGCCATTTCAAATCACACCCTTGTTGTGGTGGAAGCAGATGGAAACTACGTGCACCCATTCACGGTTGATAGCATAGACATCTACTCAGGCGAAACCTACTCAGTCCTTCTACACACGGACCAAGATCCCAACCAAAACTACTGGCTCTCAATCGGCATTATAAGCAGAAAATCAGACACACCACAAGCCCTAACAATCCTTAACTATAATACAAACCCTGCCTCAACTTTCCCTATATTACCACCACCAATCACTCCCTTATGGAACGATTTCGAGCGCAGCAAAGCATTCACAAAACAAATCATTTCCAGAATGGGAACACCACAGACACCAAAAATCGCAGACCGCACAATTTACCTCCTCAACACACAAAGCAAAATCGGTAATTTCACTAAATGGGCTATTAATAATGTTTCCTTAACATTGCCAATAACACCTTACTTAGGAGCAATCAAATATAAACTAAACAATACATTTGATAGAAATCCACCACCAGATAGATTCCCATTTGATTTCGACATATTTAAAACTCCAGTTAATCCAAGTACAACAACGGGTAACGGTGTTTACACGTTTCACTTAAATGAGGTTGTTGATGTGATACTACAAAATGCAAACATGTTAAAAGATAATACTAGTGAAGTTCATCCATGGCATTTGCATGGACATGATTTTTGGGTTTTGGGATATGGTGAAGGGAAATTTCAACCAGGTGTTCATCAGAAAAGTTTGAATTTGACACGTGCACCTTTGAGGAACACTGCAGTTATATTTCCTTATGGTTGGACTGCATTAAGGTTTAAGGCAGACAACCCTGGTGTTTGGGCATTTCATTGTCACATTGAACCACATTTGCATCTGGGAATGGGTGTTATTTTTGCTGAGGGTGTTCATAAAATTGGGAACATACCTACACATGCTCTTACTTGTGGTGCTACAGCCAAGATGTTCCTAAACAACACCCACAACTGA
Protein sequence

>Msa0450130

MDMSLLINGRGQFNCSLAANFVNTTLPQCNFKGGEECAPKILEVAPKKTYRIRIASTTSLASLNLAISNHTLVVVEADGNYVHPFTVDSIDIYSGETYSVLLHTDQDPNQNYWLSIGIISRKSDTPQALTILNYNTNPASTFPILPPPITPLWNDFERSKAFTKQIISRMGTPQTPKIADRTIYLLNTQSKIGNFTKWAINNVSLTLPITPYLGAIKYKLNNTFDRNPPPDRFPFDFDIFKTPVNPSTTTGNGVYTFHLNEVVDVILQNANMLKDNTSEVHPWHLHGHDFWVLGYGEGKFQPGVHQKSLNLTRAPLRNTAVIFPYGWTALRFKADNPGVWAFHCHIEPHLHLGMGVIFAEGVHKIGNIPTHALTCGATAKMFLNNTHN*