AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0457910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0457910 MtrunA17_Chr8g0360271 88.462 104 12 0 57 160 1 104 6.34e-63 195
Msa0457910 MtrunA17_Chr3g0110301 80.851 47 9 0 114 160 26 72 1.70e-21 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCGTTGGAAAAGTTTA+AGG 0.170316 3_3:+53085760 Msa0457910:three_prime_UTR
CTGAAGCATAACACCGGATT+TGG 0.286208 3_3:+53084870 Msa0457910:CDS
GATATAACCTGTATTTGTTT+AGG 0.291138 3_3:+53085855 Msa0457910:three_prime_UTR
AGAACACTGAGAAGCGAAAA+TGG 0.300690 3_3:+53083216 Msa0457910:CDS
AAAACTTTCGTTACAAAATC+AGG 0.317317 3_3:-53084669 None:intergenic
CTCGATAAGAGGAAAATAAA+AGG 0.319977 3_3:-53086031 None:intergenic
ATCTTTGCCTTTAGTTGATC+AGG 0.321053 3_3:-53084810 None:intergenic
CATCTGTGTTTGGTGGGTCT+TGG 0.324547 3_3:-53085532 None:intergenic
AAGAAGAAGCAAGGTGTAGA+TGG 0.344753 3_3:-53083285 None:intergenic
ATCTGTGTTTGGTGGGTCTT+GGG 0.406768 3_3:-53085531 None:intergenic
CTAGTATGAAACACACCATT+TGG 0.419336 3_3:-53084732 None:intergenic
GTTACTGCAACTGAAGAATC+TGG 0.419929 3_3:+53084915 Msa0457910:CDS
GTGTGACCATTACAAGTTGC+TGG 0.423848 3_3:+53085457 Msa0457910:three_prime_UTR
TACTTATCTAGTATTTGTAT+AGG 0.429679 3_3:+53085920 Msa0457910:three_prime_UTR
GGATGGGTACTTAGGCTCTA+AGG 0.431894 3_3:+53085833 Msa0457910:three_prime_UTR
AAAAGTTTAAGGATTCTGAT+TGG 0.433232 3_3:+53085771 Msa0457910:three_prime_UTR
CTTCTTAACCTATCATCATA+TGG 0.434087 3_3:-53085632 None:intergenic
TTATTTCCAGCAACTTGTAA+TGG 0.434209 3_3:-53085463 None:intergenic
GACATTTCAGATGATTCCTA+TGG 0.446146 3_3:+53085737 Msa0457910:three_prime_UTR
TTGATACGGTCGATTAGTTA+TGG 0.456557 3_3:-53084787 None:intergenic
TTCAGCATTCCAGCCAAATC+CGG 0.456604 3_3:-53084883 None:intergenic
AGGATAGTCCATATGATGAT+AGG 0.461215 3_3:+53085624 Msa0457910:three_prime_UTR
CAATACATCAACAATGGGAA+TGG 0.467040 3_3:+53085575 Msa0457910:three_prime_UTR
AAGTGTGTGGCTGCTCTCGA+TGG 0.472530 3_3:+53085707 Msa0457910:three_prime_UTR
ACTAGAACCTAAACAAATAC+AGG 0.475962 3_3:-53085862 None:intergenic
CATCCACGCTTCCTCTCATT+AGG 0.478275 3_3:-53085815 None:intergenic
TTTCGTCATCTGTGTTTGGT+GGG 0.480692 3_3:-53085538 None:intergenic
AGCATAACACCGGATTTGGC+TGG 0.484895 3_3:+53084874 Msa0457910:CDS
TAGAGAAGTAAACAATCATA+AGG 0.485275 3_3:-53085945 None:intergenic
GCTGCACAGTTTCAAAAGAA+AGG 0.486109 3_3:+53085434 Msa0457910:three_prime_UTR
ATGATCAATACATCAACAAT+GGG 0.488653 3_3:+53085570 Msa0457910:three_prime_UTR
ATGGTTGATGAAGTTACAAA+AGG 0.489652 3_3:+53084702 Msa0457910:CDS
TAATCATGTGCATGTTGATA+AGG 0.492675 3_3:+53085604 Msa0457910:three_prime_UTR
GTTTAAGGATTCTGATTGGA+GGG 0.496068 3_3:+53085775 Msa0457910:three_prime_UTR
ACAAAAGGAAATATGCCAAA+TGG 0.496204 3_3:+53084717 Msa0457910:CDS
ACATTAATGAGACAAAGCTT+TGG 0.497947 3_3:+53084646 Msa0457910:CDS
TATACAAATACTAGATAAGT+AGG 0.501973 3_3:-53085918 None:intergenic
CATATGATGATAGGTTAAGA+AGG 0.510751 3_3:+53085633 Msa0457910:three_prime_UTR
TTTAGTTGATCAGGTTGATA+CGG 0.511505 3_3:-53084801 None:intergenic
AATACATCAACAATGGGAAT+GGG 0.512612 3_3:+53085576 Msa0457910:three_prime_UTR
CTGCACAGTTTCAAAAGAAA+GGG 0.519886 3_3:+53085435 Msa0457910:three_prime_UTR
TGAATAGAACTCACGATACA+TGG 0.530708 3_3:-53084847 None:intergenic
GGAAGCGTGGATGGGTACTT+AGG 0.537157 3_3:+53085825 Msa0457910:three_prime_UTR
CTAATGAGAGGAAGCGTGGA+TGG 0.541911 3_3:+53085816 Msa0457910:three_prime_UTR
GATGATCAATACATCAACAA+TGG 0.551531 3_3:+53085569 Msa0457910:three_prime_UTR
ATGATAGGTTAAGAAGGGAT+AGG 0.551804 3_3:+53085639 Msa0457910:three_prime_UTR
GTATCAACCTGATCAACTAA+AGG 0.566076 3_3:+53084803 Msa0457910:CDS
AGTTTAAGGATTCTGATTGG+AGG 0.570217 3_3:+53085774 Msa0457910:three_prime_UTR
ATCGAGAGCAGCCACACACT+TGG 0.570899 3_3:-53085705 None:intergenic
AGTGTGTGGCTGCTCTCGAT+GGG 0.579630 3_3:+53085708 Msa0457910:three_prime_UTR
AACTAAAGGCAAAGATGCAT+AGG 0.582306 3_3:+53084817 Msa0457910:CDS
TGATTCCTATGGAAAAGCGT+TGG 0.584496 3_3:+53085748 Msa0457910:three_prime_UTR
AGCGAAAATGGCGATTTCGA+CGG 0.590473 3_3:+53083228 Msa0457910:CDS
TAATGAGAGGAAGCGTGGAT+GGG 0.591816 3_3:+53085817 Msa0457910:three_prime_UTR
TTGTTCTCTTACCAAGTGTG+TGG 0.604130 3_3:+53085694 Msa0457910:three_prime_UTR
ACGCTTCCTCTCATTAGGCA+TGG 0.615135 3_3:-53085810 None:intergenic
ACCGTGCTTCTTCTTCTCTG+CGG 0.616187 3_3:+53083328 Msa0457910:CDS
ACCGCAGAGAAGAAGAAGCA+CGG 0.624330 3_3:-53083329 None:intergenic
ACTCAACTTATACATCATTG+AGG 0.624896 3_3:-53084763 None:intergenic
ATATGATGATAGGTTAAGAA+GGG 0.631753 3_3:+53085634 Msa0457910:three_prime_UTR
ATGCCTAATGAGAGGAAGCG+TGG 0.638032 3_3:+53085812 Msa0457910:three_prime_UTR
AAGAAGGGATAGGAGTGCAG+AGG 0.647414 3_3:+53085649 Msa0457910:three_prime_UTR
GTGTGTTTCATACTAGAACA+TGG 0.658064 3_3:+53084739 Msa0457910:CDS
GCGGTGGAGAAGAAGAAGCA+AGG 0.660951 3_3:-53083294 None:intergenic
ACGGTAGAGAAGAAGCGCGG+TGG 0.673948 3_3:-53083310 None:intergenic
TTATCGCCATGCCTAATGAG+AGG 0.675722 3_3:+53085804 Msa0457910:three_prime_UTR
TATTCACTGAAGCATAACAC+CGG 0.679933 3_3:+53084864 Msa0457910:CDS
AGCACGGTAGAGAAGAAGCG+CGG 0.713678 3_3:-53083313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTATATTTTAAAAAT+AGG - chr3_3:53082944-53082963 None:intergenic 0.0%
!! TATATATATATATACCATAT+TGG - chr3_3:53085092-53085111 None:intergenic 10.0%
!!! AGAATTTTTCTTTATTTCTA+AGG + chr3_3:53082671-53082690 Msa0457910:intron 15.0%
!!! AGTTTCCAATTTTTATTTAT+TGG - chr3_3:53082749-53082768 None:intergenic 15.0%
!!! ATAATTTTTGAAAAGTGTTT+TGG + chr3_3:53082955-53082974 Msa0457910:intron 15.0%
!!! TAATTTTTGAAAAGTGTTTT+GGG + chr3_3:53082956-53082975 Msa0457910:intron 15.0%
!!! TAAGTTTTTTTTATCTAAAC+AGG - chr3_3:53083659-53083678 None:intergenic 15.0%
!! TTAAATGCTTATTCAATTAA+TGG - chr3_3:53084207-53084226 None:intergenic 15.0%
!!! TTTCTAAGGTAACATTTTTT+TGG + chr3_3:53082685-53082704 Msa0457910:intron 20.0%
!! GTCTTCCAATAAATAAAAAT+TGG + chr3_3:53082741-53082760 Msa0457910:intron 20.0%
!! AATAACCAAATTATTGATGT+TGG + chr3_3:53082780-53082799 Msa0457910:intron 20.0%
!! AGTTCACTAATTAAACAATA+AGG + chr3_3:53082845-53082864 Msa0457910:intron 20.0%
!! GTTCACTAATTAAACAATAA+GGG + chr3_3:53082846-53082865 Msa0457910:intron 20.0%
!!! TTTTAAAAAGTGTTTCAAAC+AGG - chr3_3:53082872-53082891 None:intergenic 20.0%
!! AAATAAGAAAAGTGAAAACA+CGG + chr3_3:53083068-53083087 Msa0457910:intron 20.0%
!! AGTAAAAAAATCGCTATATT+AGG - chr3_3:53083431-53083450 None:intergenic 20.0%
!! AAAAACACAAAATCATAGAA+CGG - chr3_3:53083483-53083502 None:intergenic 20.0%
!!! TTGAATTTTACCACTTTTAA+TGG + chr3_3:53084964-53084983 Msa0457910:intron 20.0%
!!! TGAATTTTACCACTTTTAAT+GGG + chr3_3:53084965-53084984 Msa0457910:intron 20.0%
!! ATTTATCATAATTCCAACAA+GGG - chr3_3:53085069-53085088 None:intergenic 20.0%
!! GAATTATGATAAATCCAATA+TGG + chr3_3:53085075-53085094 Msa0457910:intron 20.0%
!! TATAATCATTTGGTACATAT+TGG + chr3_3:53085172-53085191 Msa0457910:intron 20.0%
!! AAATACAAACAAACACAATA+TGG - chr3_3:53085286-53085305 None:intergenic 20.0%
!! TATACAAATACTAGATAAGT+AGG - chr3_3:53085921-53085940 None:intergenic 20.0%
!! TACTTATCTAGTATTTGTAT+AGG + chr3_3:53085920-53085939 Msa0457910:three_prime_UTR 20.0%
! ATAAAAATTGGAAACTTGAG+TGG + chr3_3:53082753-53082772 Msa0457910:intron 25.0%
! TATGAAAAACCAGATAACTA+TGG - chr3_3:53082809-53082828 None:intergenic 25.0%
!!! TGTTTTGGGTTTTTTAGTTT+TGG + chr3_3:53082970-53082989 Msa0457910:intron 25.0%
! AAAAAGTGAAAGAGCTAAAA+TGG + chr3_3:53082999-53083018 Msa0457910:intron 25.0%
! AAAAGTGAAAGAGCTAAAAT+GGG + chr3_3:53083000-53083019 Msa0457910:intron 25.0%
! TAAAAAAATGATAATACCCC+TGG - chr3_3:53083143-53083162 None:intergenic 25.0%
!! TCTATGTAAATTTGAGATTG+CGG + chr3_3:53083507-53083526 Msa0457910:intron 25.0%
! TGTATCTTTCAATATTCACA+CGG + chr3_3:53083916-53083935 Msa0457910:intron 25.0%
! AAATATGTAGCCATCTATTA+GGG - chr3_3:53084106-53084125 None:intergenic 25.0%
!! CAAAAATATTCGTGTTTTCT+CGG + chr3_3:53084396-53084415 Msa0457910:intron 25.0%
! GACTAAACTGTAAAAATAGA+AGG - chr3_3:53084452-53084471 None:intergenic 25.0%
!!! ATGCGTATTTTTATGATAGA+TGG + chr3_3:53084611-53084630 Msa0457910:intron 25.0%
! AAAACTTTCGTTACAAAATC+AGG - chr3_3:53084672-53084691 None:intergenic 25.0%
!! GAAAGTTTTCATTGACATAA+TGG + chr3_3:53084683-53084702 Msa0457910:CDS 25.0%
! AATCTGGAAAAATTATCTGA+AGG + chr3_3:53084931-53084950 Msa0457910:CDS 25.0%
! GATTTATCATAATTCCAACA+AGG - chr3_3:53085070-53085089 None:intergenic 25.0%
!!! TTTCATTGATTCTCTTACTT+AGG + chr3_3:53085349-53085368 Msa0457910:intron 25.0%
! ATACAATGAGAATAACAGAA+AGG - chr3_3:53085377-53085396 None:intergenic 25.0%
! TGATATGCATATATCATTTC+AGG + chr3_3:53085400-53085419 Msa0457910:intron 25.0%
! ATGATCAATACATCAACAAT+GGG + chr3_3:53085570-53085589 Msa0457910:three_prime_UTR 25.0%
! ATATGATGATAGGTTAAGAA+GGG + chr3_3:53085634-53085653 Msa0457910:three_prime_UTR 25.0%
! AAAAGTTTAAGGATTCTGAT+TGG + chr3_3:53085771-53085790 Msa0457910:three_prime_UTR 25.0%
! GATATAACCTGTATTTGTTT+AGG + chr3_3:53085855-53085874 Msa0457910:three_prime_UTR 25.0%
! TAGAGAAGTAAACAATCATA+AGG - chr3_3:53085948-53085967 None:intergenic 25.0%
GGTAACCAACATCAATAATT+TGG - chr3_3:53082788-53082807 None:intergenic 30.0%
!!! AAAAGTGTCACTTTTCTCAA+TGG + chr3_3:53083093-53083112 Msa0457910:intron 30.0%
! TGTAATTATGTTTGAAGTCC+AGG + chr3_3:53083122-53083141 Msa0457910:intron 30.0%
! GTAATTATGTTTGAAGTCCA+GGG + chr3_3:53083123-53083142 Msa0457910:intron 30.0%
! TAATTATGTTTGAAGTCCAG+GGG + chr3_3:53083124-53083143 Msa0457910:intron 30.0%
!! TTAGTTTTTCTGCAGAATGA+GGG - chr3_3:53083192-53083211 None:intergenic 30.0%
TGTAGATGGAAGAAAAATCA+CGG - chr3_3:53083274-53083293 None:intergenic 30.0%
GATTGATATTTGCAGCTTAT+AGG + chr3_3:53083620-53083639 Msa0457910:intron 30.0%
CAAATATGTAGCCATCTATT+AGG - chr3_3:53084107-53084126 None:intergenic 30.0%
TAGATGGCTACATATTTGTT+TGG + chr3_3:53084109-53084128 Msa0457910:intron 30.0%
TTTACTAACAAGACAAGAAG+TGG - chr3_3:53084317-53084336 None:intergenic 30.0%
AGAGAGAGCAAATAAAATCA+AGG - chr3_3:53084506-53084525 None:intergenic 30.0%
!! TTGATTTTATTTGCTCTCTC+TGG + chr3_3:53084505-53084524 Msa0457910:intron 30.0%
ACATTAATGAGACAAAGCTT+TGG + chr3_3:53084646-53084665 Msa0457910:CDS 30.0%
!! ATGGTTGATGAAGTTACAAA+AGG + chr3_3:53084702-53084721 Msa0457910:CDS 30.0%
ACAAAAGGAAATATGCCAAA+TGG + chr3_3:53084717-53084736 Msa0457910:CDS 30.0%
ACTCAACTTATACATCATTG+AGG - chr3_3:53084766-53084785 None:intergenic 30.0%
!! TTTAGTTGATCAGGTTGATA+CGG - chr3_3:53084804-53084823 None:intergenic 30.0%
GGAAAAATTATCTGAAGGTA+TGG + chr3_3:53084936-53084955 Msa0457910:intron 30.0%
ATCATATCACCCATTAAAAG+TGG - chr3_3:53084977-53084996 None:intergenic 30.0%
CAAGATTCATGATTCTAGAT+AGG - chr3_3:53085311-53085330 None:intergenic 30.0%
TTATTTCCAGCAACTTGTAA+TGG - chr3_3:53085466-53085485 None:intergenic 30.0%
TTCAACAAAAACAATGCAAC+TGG + chr3_3:53085488-53085507 Msa0457910:three_prime_UTR 30.0%
GATGATCAATACATCAACAA+TGG + chr3_3:53085569-53085588 Msa0457910:three_prime_UTR 30.0%
AATACATCAACAATGGGAAT+GGG + chr3_3:53085576-53085595 Msa0457910:three_prime_UTR 30.0%
TAATCATGTGCATGTTGATA+AGG + chr3_3:53085604-53085623 Msa0457910:three_prime_UTR 30.0%
CTTCTTAACCTATCATCATA+TGG - chr3_3:53085635-53085654 None:intergenic 30.0%
CATATGATGATAGGTTAAGA+AGG + chr3_3:53085633-53085652 Msa0457910:three_prime_UTR 30.0%
!! AAAAGCGTTGGAAAAGTTTA+AGG + chr3_3:53085760-53085779 Msa0457910:three_prime_UTR 30.0%
ACTAGAACCTAAACAAATAC+AGG - chr3_3:53085865-53085884 None:intergenic 30.0%
! TAGTGTCATTATTGTCACGA+TGG + chr3_3:53082715-53082734 Msa0457910:intron 35.0%
! TGTTGGTTACCATAGTTATC+TGG + chr3_3:53082797-53082816 Msa0457910:intron 35.0%
!!! GTTAGTTTTTCTGCAGAATG+AGG - chr3_3:53083193-53083212 None:intergenic 35.0%
ATATTTGCAGCTTATAGGTC+TGG + chr3_3:53083625-53083644 Msa0457910:intron 35.0%
TTCAATATTCACACGGATAC+CGG + chr3_3:53083923-53083942 Msa0457910:intron 35.0%
TGCACACAAGAACTAGAAAA+AGG - chr3_3:53084069-53084088 None:intergenic 35.0%
TATGCTTGTTCCCTAATAGA+TGG + chr3_3:53084093-53084112 Msa0457910:intron 35.0%
TTATCTGTTAGTCACCTTTG+AGG + chr3_3:53084235-53084254 Msa0457910:intron 35.0%
! TCTTGTCTTGTTAGTAAAGC+TGG + chr3_3:53084319-53084338 Msa0457910:intron 35.0%
CAAGACTTGGCACAAAAATA+AGG - chr3_3:53084360-53084379 None:intergenic 35.0%
TATCTGTCAGATACAAGACT+TGG - chr3_3:53084373-53084392 None:intergenic 35.0%
CTAGTATGAAACACACCATT+TGG - chr3_3:53084735-53084754 None:intergenic 35.0%
GTGTGTTTCATACTAGAACA+TGG + chr3_3:53084739-53084758 Msa0457910:CDS 35.0%
! TTGATACGGTCGATTAGTTA+TGG - chr3_3:53084790-53084809 None:intergenic 35.0%
GTATCAACCTGATCAACTAA+AGG + chr3_3:53084803-53084822 Msa0457910:CDS 35.0%
ATCTTTGCCTTTAGTTGATC+AGG - chr3_3:53084813-53084832 None:intergenic 35.0%
AACTAAAGGCAAAGATGCAT+AGG + chr3_3:53084817-53084836 Msa0457910:CDS 35.0%
TGAATAGAACTCACGATACA+TGG - chr3_3:53084850-53084869 None:intergenic 35.0%
TATTCACTGAAGCATAACAC+CGG + chr3_3:53084864-53084883 Msa0457910:CDS 35.0%
TCATGCTTCTATTAGTGACA+TGG + chr3_3:53085022-53085041 Msa0457910:intron 35.0%
TCACATTTCAATACCCTTGT+TGG + chr3_3:53085053-53085072 Msa0457910:intron 35.0%
TTAGCGCAATGAACAAAACT+GGG - chr3_3:53085217-53085236 None:intergenic 35.0%
TTTAGCGCAATGAACAAAAC+TGG - chr3_3:53085218-53085237 None:intergenic 35.0%
!!! TTTTGAAACTGTGCAGCTTT+GGG - chr3_3:53085431-53085450 None:intergenic 35.0%
CTGCACAGTTTCAAAAGAAA+GGG + chr3_3:53085435-53085454 Msa0457910:three_prime_UTR 35.0%
CAATACATCAACAATGGGAA+TGG + chr3_3:53085575-53085594 Msa0457910:three_prime_UTR 35.0%
AGGATAGTCCATATGATGAT+AGG + chr3_3:53085624-53085643 Msa0457910:three_prime_UTR 35.0%
ATGATAGGTTAAGAAGGGAT+AGG + chr3_3:53085639-53085658 Msa0457910:three_prime_UTR 35.0%
GACATTTCAGATGATTCCTA+TGG + chr3_3:53085737-53085756 Msa0457910:three_prime_UTR 35.0%
AGTTTAAGGATTCTGATTGG+AGG + chr3_3:53085774-53085793 Msa0457910:three_prime_UTR 35.0%
GTTTAAGGATTCTGATTGGA+GGG + chr3_3:53085775-53085794 Msa0457910:three_prime_UTR 35.0%
AGAACACTGAGAAGCGAAAA+TGG + chr3_3:53083216-53083235 Msa0457910:CDS 40.0%
AGATGGAAGAAAAATCACGG+CGG - chr3_3:53083271-53083290 None:intergenic 40.0%
AAGAAGAAGCAAGGTGTAGA+TGG - chr3_3:53083288-53083307 None:intergenic 40.0%
!!! TTCTTCTCTGCGGTTTTTTC+AGG + chr3_3:53083338-53083357 Msa0457910:CDS 40.0%
GTTTGAGCTCAATACCTCAA+AGG - chr3_3:53084252-53084271 None:intergenic 40.0%
TCAAACAGCTTAGCCAAACT+GGG + chr3_3:53084266-53084285 Msa0457910:intron 40.0%
TTTACCTGTACAACCCAGTT+TGG - chr3_3:53084282-53084301 None:intergenic 40.0%
GGTTGTACAGGTAAAGAACA+GGG + chr3_3:53084287-53084306 Msa0457910:intron 40.0%
GTTGTACAGGTAAAGAACAG+GGG + chr3_3:53084288-53084307 Msa0457910:intron 40.0%
GCACAAAAATAAGGCTACCT+AGG - chr3_3:53084351-53084370 None:intergenic 40.0%
!!! CTCTCTGGTGCTACTTTTAT+AGG + chr3_3:53084520-53084539 Msa0457910:intron 40.0%
GTTACTGCAACTGAAGAATC+TGG + chr3_3:53084915-53084934 Msa0457910:CDS 40.0%
!!! CTTTTGAAACTGTGCAGCTT+TGG - chr3_3:53085432-53085451 None:intergenic 40.0%
GCTGCACAGTTTCAAAAGAA+AGG + chr3_3:53085434-53085453 Msa0457910:three_prime_UTR 40.0%
TTTCGTCATCTGTGTTTGGT+GGG - chr3_3:53085541-53085560 None:intergenic 40.0%
! TTTTCGTCATCTGTGTTTGG+TGG - chr3_3:53085542-53085561 None:intergenic 40.0%
! TCGTTTTCGTCATCTGTGTT+TGG - chr3_3:53085545-53085564 None:intergenic 40.0%
TTGTTCTCTTACCAAGTGTG+TGG + chr3_3:53085694-53085713 Msa0457910:three_prime_UTR 40.0%
! TGATTCCTATGGAAAAGCGT+TGG + chr3_3:53085748-53085767 Msa0457910:three_prime_UTR 40.0%
! CTTTTCCAACGCTTTTCCAT+AGG - chr3_3:53085756-53085775 None:intergenic 40.0%
AGCGAAAATGGCGATTTCGA+CGG + chr3_3:53083228-53083247 Msa0457910:CDS 45.0%
AGAAAAATCACGGCGGAGAT+CGG - chr3_3:53083264-53083283 None:intergenic 45.0%
!! CCGTTGACGAGTTTTGTATC+CGG - chr3_3:53083945-53083964 None:intergenic 45.0%
CCGGATACAAAACTCGTCAA+CGG + chr3_3:53083942-53083961 Msa0457910:intron 45.0%
CTCAAACAGCTTAGCCAAAC+TGG + chr3_3:53084265-53084284 Msa0457910:intron 45.0%
TTAGCCAAACTGGGTTGTAC+AGG + chr3_3:53084275-53084294 Msa0457910:intron 45.0%
GGGTTGTACAGGTAAAGAAC+AGG + chr3_3:53084286-53084305 Msa0457910:intron 45.0%
AGTAAAGCTGGACTGAACCT+AGG + chr3_3:53084331-53084350 Msa0457910:intron 45.0%
CTGAAGCATAACACCGGATT+TGG + chr3_3:53084870-53084889 Msa0457910:CDS 45.0%
TTCAGCATTCCAGCCAAATC+CGG - chr3_3:53084886-53084905 None:intergenic 45.0%
GTGTGACCATTACAAGTTGC+TGG + chr3_3:53085457-53085476 Msa0457910:three_prime_UTR 45.0%
! ATCTGTGTTTGGTGGGTCTT+GGG - chr3_3:53085534-53085553 None:intergenic 45.0%
TTATCGCCATGCCTAATGAG+AGG + chr3_3:53085804-53085823 Msa0457910:three_prime_UTR 45.0%
! TAATGAGAGGAAGCGTGGAT+GGG + chr3_3:53085817-53085836 Msa0457910:three_prime_UTR 45.0%
!!! ATTTTTTTATCAAAAAAATT+TGG + chr3_3:53083154-53083173 Msa0457910:intron 5.0%
!! TATATATATATATAATCATT+TGG + chr3_3:53085162-53085181 Msa0457910:intron 5.0%
ACCGTGCTTCTTCTTCTCTG+CGG + chr3_3:53083328-53083347 Msa0457910:CDS 50.0%
ACCGCAGAGAAGAAGAAGCA+CGG - chr3_3:53083332-53083351 None:intergenic 50.0%
AGCATAACACCGGATTTGGC+TGG + chr3_3:53084874-53084893 Msa0457910:CDS 50.0%
! CATCTGTGTTTGGTGGGTCT+TGG - chr3_3:53085535-53085554 None:intergenic 50.0%
! AAGAAGGGATAGGAGTGCAG+AGG + chr3_3:53085649-53085668 Msa0457910:three_prime_UTR 50.0%
ACGCTTCCTCTCATTAGGCA+TGG - chr3_3:53085813-53085832 None:intergenic 50.0%
ATGCCTAATGAGAGGAAGCG+TGG + chr3_3:53085812-53085831 Msa0457910:three_prime_UTR 50.0%
CATCCACGCTTCCTCTCATT+AGG - chr3_3:53085818-53085837 None:intergenic 50.0%
! CTAATGAGAGGAAGCGTGGA+TGG + chr3_3:53085816-53085835 Msa0457910:three_prime_UTR 50.0%
GGATGGGTACTTAGGCTCTA+AGG + chr3_3:53085833-53085852 Msa0457910:three_prime_UTR 50.0%
GCGGTGGAGAAGAAGAAGCA+AGG - chr3_3:53083297-53083316 None:intergenic 55.0%
AGCACGGTAGAGAAGAAGCG+CGG - chr3_3:53083316-53083335 None:intergenic 55.0%
ATCGAGAGCAGCCACACACT+TGG - chr3_3:53085708-53085727 None:intergenic 55.0%
AAGTGTGTGGCTGCTCTCGA+TGG + chr3_3:53085707-53085726 Msa0457910:three_prime_UTR 55.0%
AGTGTGTGGCTGCTCTCGAT+GGG + chr3_3:53085708-53085727 Msa0457910:three_prime_UTR 55.0%
! GGAAGCGTGGATGGGTACTT+AGG + chr3_3:53085825-53085844 Msa0457910:three_prime_UTR 55.0%
! ACGGTAGAGAAGAAGCGCGG+TGG - chr3_3:53083313-53083332 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 53082654 53086043 53082654 ID=Msa0457910;Name=Msa0457910
chr3_3 mRNA 53082654 53086043 53082654 ID=Msa0457910-mRNA-1;Parent=Msa0457910;Name=Msa0457910-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0.25|0.4|0.6|0.75|0.8|5|624|162
chr3_3 exon 53082654 53082658 53082654 ID=Msa0457910-mRNA-1:exon:10308;Parent=Msa0457910-mRNA-1
chr3_3 exon 53083203 53083359 53083203 ID=Msa0457910-mRNA-1:exon:10309;Parent=Msa0457910-mRNA-1
chr3_3 exon 53083506 53083511 53083506 ID=Msa0457910-mRNA-1:exon:10310;Parent=Msa0457910-mRNA-1
chr3_3 exon 53084630 53084952 53084630 ID=Msa0457910-mRNA-1:exon:10311;Parent=Msa0457910-mRNA-1
chr3_3 exon 53085422 53086043 53085422 ID=Msa0457910-mRNA-1:exon:10312;Parent=Msa0457910-mRNA-1
chr3_3 CDS 53082654 53082658 53082654 ID=Msa0457910-mRNA-1:cds;Parent=Msa0457910-mRNA-1
chr3_3 CDS 53083203 53083359 53083203 ID=Msa0457910-mRNA-1:cds;Parent=Msa0457910-mRNA-1
chr3_3 CDS 53083506 53083511 53083506 ID=Msa0457910-mRNA-1:cds;Parent=Msa0457910-mRNA-1
chr3_3 CDS 53084630 53084950 53084630 ID=Msa0457910-mRNA-1:cds;Parent=Msa0457910-mRNA-1
chr3_3 three_prime_UTR 53084951 53084952 53084951 ID=Msa0457910-mRNA-1:three_prime_utr;Parent=Msa0457910-mRNA-1
chr3_3 three_prime_UTR 53085422 53086043 53085422 ID=Msa0457910-mRNA-1:three_prime_utr;Parent=Msa0457910-mRNA-1
Gene Sequence

>Msa0457910

ATGAAAAAAACTAACGTGAGAACACTGAGAAGCGAAAATGGCGATTTCGACGGCGATTCTTCTCCGATCTCCGCCGTGATTTTTCTTCCATCTACACCTTGCTTCTTCTTCTCCACCGCGCTTCTTCTCTACCGTGCTTCTTCTTCTCTGCGGTTTTTTCAGATCTATATGGCAACTAATGTTGACATTAATGAGACAAAGCTTTGGCCTGATTTTGTAACGAAAGTTTTCATTGACATAATGGTTGATGAAGTTACAAAAGGAAATATGCCAAATGGTGTGTTTCATACTAGAACATGGTCCTCAATGATGTATAAGTTGAGTACCATAACTAATCGACCGTATCAACCTGATCAACTAAAGGCAAAGATGCATAGGTTGCGAGCCATGTATCGTGAGTTCTATTCACTGAAGCATAACACCGGATTTGGCTGGAATGCTGAAACAAACACTGTTACTGCAACTGAAGAATCTGGAAAAATTATCTGA
Protein sequence

>Msa0457910

MKKTNVRTLRSENGDFDGDSSPISAVIFLPSTPCFFFSTALLLYRASSSLRFFQIYMATNVDINETKLWPDFVTKVFIDIMVDEVTKGNMPNGVFHTRTWSSMMYKLSTITNRPYQPDQLKAKMHRLRAMYREFYSLKHNTGFGWNAETNTVTATEESGKII*