AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0460850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0460850 MtrunA17_Chr3g0078891 61.458 384 131 8 1 379 1 372 3.26e-144 415
Msa0460850 MtrunA17_Chr3g0078731 61.257 382 131 9 8 379 2 376 2.30e-142 409
Msa0460850 MtrunA17_Chr8g0364381 59.481 385 138 9 1 376 49 424 3.17e-135 393
Msa0460850 MtrunA17_Chr3g0078681 64.537 313 96 7 10 312 18 325 6.63e-126 365
Msa0460850 MtrunA17_Chr3g0077781 55.467 375 146 9 8 378 9 366 5.48e-121 354
Msa0460850 MtrunA17_Chr2g0325081 49.046 367 170 7 15 378 13 365 2.35e-101 304
Msa0460850 MtrunA17_Chr2g0331741 48.806 377 173 8 5 377 69 429 3.74e-100 303
Msa0460850 MtrunA17_Chr3g0078191 47.520 383 171 10 5 378 37 398 9.06e-95 288
Msa0460850 MtrunA17_Chr3g0077791 54.683 331 128 6 5 325 6 324 1.67e-94 285
Msa0460850 MtrunA17_Chr3g0085901 46.113 373 168 7 7 377 3 344 4.87e-91 277
Msa0460850 MtrunA17_Chr5g0427711 45.232 367 178 7 15 378 3 349 1.28e-80 251
Msa0460850 MtrunA17_Chr7g0249591 41.818 385 182 9 5 377 3 357 3.56e-74 236
Msa0460850 MtrunA17_Chr3g0082431 41.918 365 186 8 15 377 10 350 1.13e-72 231
Msa0460850 MtrunA17_Chr3g0082451 41.644 365 186 8 15 377 10 349 3.51e-72 229
Msa0460850 MtrunA17_Chr6g0457681 39.851 404 176 12 3 379 42 405 1.51e-70 227
Msa0460850 MtrunA17_Chr7g0221741 39.898 391 184 11 1 377 1 354 4.32e-67 216
Msa0460850 MtrunA17_Chr3g0085941 40.921 369 184 11 15 375 14 356 8.41e-67 215
Msa0460850 MtrunA17_Chr7g0221701 41.261 349 158 10 38 377 4 314 2.41e-66 213
Msa0460850 MtrunA17_Chr5g0424761 41.918 365 186 9 15 377 17 357 5.23e-66 213
Msa0460850 MtrunA17_Chr7g0252201 45.230 283 134 6 10 289 11 275 3.66e-65 209
Msa0460850 MtrunA17_Chr8g0366411 38.554 415 188 11 1 377 65 450 2.72e-64 211
Msa0460850 MtrunA17_Chr4g0070811 46.043 278 133 7 103 377 5 268 1.20e-63 204
Msa0460850 MtrunA17_Chr3g0091201 40.476 336 166 7 1 325 11 323 2.79e-62 202
Msa0460850 MtrunA17_Chr4g0049031 38.619 391 186 11 15 377 28 392 2.34e-61 205
Msa0460850 MtrunA17_Chr3g0085961 35.222 406 210 9 2 377 1 383 1.47e-60 200
Msa0460850 MtrunA17_Chr6g0457641 40.705 312 163 7 15 325 51 341 1.66e-58 194
Msa0460850 MtrunA17_Chr3g0084261 37.783 397 192 11 11 377 21 392 9.68e-58 193
Msa0460850 MtrunA17_Chr3g0132661 35.096 416 188 14 10 377 20 401 4.08e-56 189
Msa0460850 MtrunA17_Chr3g0084241 39.175 388 182 13 15 375 7 367 9.77e-56 187
Msa0460850 MtrunA17_Chr3g0085951 36.816 402 192 11 14 378 1 377 8.19e-55 186
Msa0460850 MtrunA17_Chr6g0460081 36.667 390 199 12 3 378 22 377 9.85e-54 182
Msa0460850 MtrunA17_Chr6g0486911 34.588 425 200 12 1 374 4 401 4.24e-52 178
Msa0460850 MtrunA17_Chr5g0419301 34.398 407 206 10 7 374 21 405 4.33e-52 179
Msa0460850 MtrunA17_Chr4g0048151 32.938 422 208 12 3 377 17 410 2.38e-51 178
Msa0460850 MtrunA17_Chr6g0457621 34.634 410 180 13 3 378 22 377 3.90e-51 175
Msa0460850 MtrunA17_Chr4g0005311 34.975 406 192 10 15 378 25 400 4.39e-51 176
Msa0460850 MtrunA17_Chr4g0049111 35.165 364 200 10 5 358 32 369 1.40e-50 176
Msa0460850 MtrunA17_Chr6g0460041 39.194 273 144 7 16 287 41 292 3.20e-50 170
Msa0460850 MtrunA17_Chr4g0049041 34.118 425 193 15 8 376 17 410 7.59e-50 173
Msa0460850 MtrunA17_Chr5g0440681 32.812 384 220 8 4 377 14 369 1.15e-49 171
Msa0460850 MtrunA17_Chr6g0486921 32.494 437 207 12 5 376 8 421 2.48e-49 171
Msa0460850 MtrunA17_Chr4g0049121 35.000 420 214 14 1 374 17 423 2.89e-49 174
Msa0460850 MtrunA17_Chr4g0023631 34.826 402 211 10 4 378 7 384 8.64e-49 171
Msa0460850 MtrunA17_Chr5g0419311 35.671 328 178 8 7 326 21 323 3.29e-48 166
Msa0460850 MtrunA17_Chr1g0199541 38.267 277 128 8 3 265 13 260 5.29e-48 164
Msa0460850 MtrunA17_Chr3g0099441 31.757 444 199 13 15 377 27 447 1.08e-47 168
Msa0460850 MtrunA17_Chr3g0091161 38.938 339 175 11 15 349 14 324 3.91e-46 162
Msa0460850 MtrunA17_Chr5g0424701 42.727 220 116 5 15 232 16 227 5.13e-46 158
Msa0460850 MtrunA17_Chr4g0049071 32.010 403 203 12 16 374 50 425 6.17e-45 160
Msa0460850 MtrunA17_Chr3g0104721 39.259 270 124 9 11 265 24 268 3.80e-44 154
Msa0460850 MtrunA17_Chr3g0104731 38.077 260 130 5 15 265 19 256 4.78e-42 149
Msa0460850 MtrunA17_Chr1g0170171 36.503 326 157 8 1 325 10 286 5.82e-42 149
Msa0460850 MtrunA17_Chr3g0091211 34.416 308 133 5 7 309 2 245 4.65e-41 145
Msa0460850 MtrunA17_Chr3g0099461 30.788 419 200 12 15 377 27 411 7.73e-40 146
Msa0460850 MtrunA17_Chr3g0084251 43.534 232 123 6 2 231 12 237 1.69e-39 147
Msa0460850 MtrunA17_Chr3g0084251 32.767 412 205 16 8 377 189 570 2.86e-32 127
Msa0460850 MtrunA17_Chr8g0361351 31.105 389 230 13 7 379 2 368 8.24e-39 142
Msa0460850 MtrunA17_Chr5g0434961 63.717 113 39 1 78 190 2 112 2.25e-38 133
Msa0460850 MtrunA17_Chr8g0362531 32.134 389 221 14 10 379 36 400 3.46e-38 141
Msa0460850 MtrunA17_Chr1g0181881 48.649 148 74 2 6 151 9 156 5.64e-37 131
Msa0460850 MtrunA17_Chr6g0488401 32.447 376 221 13 8 370 2 357 3.61e-36 135
Msa0460850 MtrunA17_Chr4g0011401 30.280 393 223 13 5 374 135 499 2.72e-33 130
Msa0460850 MtrunA17_Chr4g0058061 31.940 335 189 9 3 332 150 450 2.48e-32 126
Msa0460850 MtrunA17_Chr6g0488371 36.036 222 116 7 8 213 3 214 3.64e-32 120
Msa0460850 MtrunA17_Chr4g0049531 31.063 367 172 11 60 375 2 338 1.08e-30 120
Msa0460850 MtrunA17_Chr4g0011331 39.565 230 115 8 5 227 146 358 2.34e-30 120
Msa0460850 MtrunA17_Chr7g0220351 29.949 394 227 11 10 378 142 511 3.78e-30 121
Msa0460850 MtrunA17_Chr6g0452031 34.783 276 131 10 5 243 2 265 2.59e-29 115
Msa0460850 MtrunA17_Chr2g0331731 41.304 184 94 5 197 378 2 173 2.67e-29 111
Msa0460850 MtrunA17_Chr4g0039521 36.653 251 130 6 1 249 1 224 8.32e-28 109
Msa0460850 MtrunA17_Chr1g0188701 32.821 195 126 2 15 208 3 193 6.06e-27 108
Msa0460850 MtrunA17_Chr4g0011321 36.681 229 108 8 3 227 19 214 1.55e-26 107
Msa0460850 MtrunA17_Chr3g0100081 28.986 345 189 12 16 325 25 348 9.65e-26 106
Msa0460850 MtrunA17_Chr3g0085981 31.818 308 148 9 106 377 8 289 6.01e-25 103
Msa0460850 MtrunA17_Chr6g0460051 48.571 105 52 1 15 117 51 155 7.77e-25 99.0
Msa0460850 MtrunA17_Chr4g0049131 47.059 102 54 0 14 115 5 106 1.36e-22 92.4
Msa0460850 MtrunA17_Chr5g0404531 35.747 221 103 8 133 349 1 186 1.76e-22 94.0
Msa0460850 MtrunA17_Chr8g0342451 28.621 290 164 9 15 263 35 322 3.18e-22 97.4
Msa0460850 MtrunA17_Chr3g0087471 43.697 119 61 2 5 117 10 128 4.90e-22 90.9
Msa0460850 MtrunA17_Chr4g0023731 36.364 132 83 1 1 131 5 136 9.40e-22 92.4
Msa0460850 MtrunA17_Chr2g0330161 28.216 241 135 8 1 204 20 259 3.70e-20 91.7
Msa0460850 MtrunA17_Chr8g0361241 39.855 138 74 5 16 145 12 148 2.32e-19 87.4
Msa0460850 MtrunA17_Chr3g0099431 32.749 171 95 5 156 325 6 157 4.43e-19 83.6
Msa0460850 MtrunA17_Chr4g0049061 40.517 116 66 2 3 115 21 136 3.93e-18 80.9
Msa0460850 MtrunA17_Chr7g0226481 27.042 355 208 14 16 360 11 324 4.74e-18 84.3
Msa0460850 MtrunA17_Chr3g0084231 48.039 102 48 1 15 111 24 125 1.82e-17 82.8
Msa0460850 MtrunA17_Chr3g0136291 31.579 171 101 4 16 180 14 174 3.47e-16 79.0
Msa0460850 MtrunA17_Chr2g0278801 31.718 227 125 8 16 225 2 215 7.15e-16 76.6
Msa0460850 MtrunA17_Chr3g0139601 33.526 173 102 4 15 184 12 174 1.65e-15 77.8
Msa0460850 MtrunA17_Chr4g0049091 27.596 337 158 14 94 374 7 313 4.80e-15 75.9
Msa0460850 MtrunA17_Chr7g0269551 35.714 126 62 3 163 288 4 110 3.60e-14 68.9
Msa0460850 MtrunA17_Chr2g0278121 33.333 189 95 7 16 180 16 197 1.31e-13 71.6
Msa0460850 MtrunA17_Chr7g0236101 31.852 135 90 1 15 147 8 142 8.23e-13 66.2
Msa0460850 MtrunA17_Chr1g0166251 30.769 169 100 5 16 178 11 168 1.14e-12 68.9
Msa0460850 MtrunA17_Chr7g0273871 29.480 173 103 6 16 180 13 174 1.32e-12 68.6
Msa0460850 MtrunA17_Chr7g0255101 27.803 223 134 7 15 227 109 314 1.44e-12 68.9
Msa0460850 MtrunA17_Chr7g0236211 30.481 187 113 6 6 184 2 179 1.84e-12 68.2
Msa0460850 MtrunA17_Chr5g0404351 37.288 118 71 3 31 145 7 124 2.56e-12 63.5
Msa0460850 MtrunA17_Chr2g0278131 44.595 74 39 1 1 74 1 72 5.11e-12 61.2
Msa0460850 MtrunA17_Chr7g0236221 41.772 79 46 0 15 93 8 86 5.18e-12 62.8
Msa0460850 MtrunA17_Chr2g0278791 32.164 171 96 6 16 178 2 160 6.82e-12 66.2
Msa0460850 MtrunA17_Chr7g0236151 31.852 135 90 1 15 147 8 142 9.80e-12 65.5
Msa0460850 MtrunA17_Chr7g0236191 31.852 135 90 1 15 147 8 142 9.80e-12 65.5
Msa0460850 MtrunA17_Chr4g0049051 32.168 143 78 4 180 322 2 125 1.08e-11 62.4
Msa0460850 MtrunA17_Chr7g0227621 43.284 67 38 0 16 82 11 77 2.25e-11 60.5
Msa0460850 MtrunA17_Chr3g0091171 29.143 175 111 5 16 184 5 172 2.51e-11 64.7
Msa0460850 MtrunA17_Chr7g0246351 30.208 192 101 7 15 180 5 189 4.95e-11 63.9
Msa0460850 MtrunA17_Chr7g0236171 46.377 69 37 0 15 83 8 76 5.07e-11 60.8
Msa0460850 MtrunA17_Chr7g0236131 46.377 69 37 0 15 83 8 76 5.07e-11 60.8
Msa0460850 MtrunA17_Chr7g0236081 46.377 69 37 0 15 83 8 76 5.07e-11 60.8
Msa0460850 MtrunA17_Chr3g0093361 26.286 175 116 4 16 184 43 210 5.73e-11 63.5
Msa0460850 MtrunA17_Chr3g0138571 28.824 170 105 4 15 180 12 169 6.12e-11 63.5
Msa0460850 MtrunA17_Chr7g0227851 28.649 185 121 4 16 197 11 187 6.31e-11 63.5
Msa0460850 MtrunA17_Chr2g0278091 32.000 200 90 8 16 210 12 170 9.49e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0460850 AT4G10400.2 32.558 172 99 6 16 180 2 163 3.53e-12 67.8
Msa0460850 AT4G10400.3 32.558 172 99 6 16 180 2 163 3.53e-12 67.8
Msa0460850 AT4G10400.1 32.558 172 99 6 16 180 2 163 3.53e-12 67.8
Msa0460850 AT1G66310.1 24.796 367 219 15 4 353 9 335 3.26e-11 65.1
Msa0460850 AT5G44950.1 29.787 141 93 2 15 149 3 143 5.16e-11 64.3
Msa0460850 AT5G53840.1 31.148 183 113 5 1 173 1 180 5.72e-11 64.3
Msa0460850 AT1G69630.1 25.778 225 133 7 16 226 18 222 6.88e-11 63.9
Msa0460850 AT5G44940.1 31.206 141 89 2 16 148 5 145 8.15e-11 63.5

Find 68 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCATTATAATTCATTTA+AGG 0.131018 3_3:-58495579 Msa0460850:CDS
TATTGCAGCTTCTATGATTT+TGG 0.167771 3_3:-58495810 Msa0460850:CDS
CAAGTTTGTTTCTCTGAATA+AGG 0.185183 3_3:+58495354 None:intergenic
GATTATATTCCAAAACATTT+TGG 0.187905 3_3:-58495875 Msa0460850:CDS
CCAGAGATTGATCCATCTTT+TGG 0.247931 3_3:+58496540 None:intergenic
AAAGATTTGGAAATGGGTTT+TGG 0.265247 3_3:+58495952 None:intergenic
TTGACTTCTCTCAGTCTTGC+TGG 0.274112 3_3:-58496259 Msa0460850:CDS
GTCTTGCTTCCAAAATGTTT+TGG 0.296893 3_3:+58495866 None:intergenic
GCAAGTTTGAACAGCCTCTT+TGG 0.311685 3_3:+58496570 None:intergenic
AGCCTCTTTGGCGTTCAAAA+AGG 0.331850 3_3:+58496582 None:intergenic
CTCTATTCAACTGGCTGGCT+TGG 0.337888 3_3:-58495772 Msa0460850:CDS
CTTGAAGTTGTTGAAATTAG+TGG 0.343944 3_3:-58495534 Msa0460850:CDS
CAACTTGTTAAATGCGGAAA+AGG 0.344718 3_3:+58496105 None:intergenic
ATGTTAGATATAGATTCTTT+TGG 0.393316 3_3:-58495399 Msa0460850:three_prime_UTR
AACTTCAAGTTATGCAAGTA+AGG 0.394364 3_3:+58495548 None:intergenic
ATTCTATAATCTTTACATAT+AGG 0.399025 3_3:+58496368 None:intergenic
ACCATCATCATTACAACAAA+AGG 0.417640 3_3:+58496138 None:intergenic
AAGTTTGTTTCTCTGAATAA+GGG 0.417966 3_3:+58495355 None:intergenic
ACCAATCAACTCTATTCAAC+TGG 0.421253 3_3:-58495781 Msa0460850:CDS
AACTTGTTAAATGCGGAAAA+GGG 0.422883 3_3:+58496106 None:intergenic
TCAAGACAGGCTCAGTGAAT+TGG 0.423260 3_3:-58496632 Msa0460850:CDS
CCAAAAGATGGATCAATCTC+TGG 0.439047 3_3:-58496540 Msa0460850:CDS
GCCAGTTGAATAGAGTTGAT+TGG 0.444802 3_3:+58495780 None:intergenic
TTTCATGTACCAATCTTGTC+AGG 0.451651 3_3:-58496024 Msa0460850:CDS
AGGCAAGGTGCTTCAGAGAC+AGG 0.453076 3_3:+58496158 None:intergenic
AGAGACAGGGTGGTTAATGC+TGG 0.459591 3_3:+58496172 None:intergenic
AAAACGTAGACATGAATTGT+TGG 0.465572 3_3:+58496461 None:intergenic
CACTAAGAGCAGCTAAATTG+AGG 0.485826 3_3:+58495726 None:intergenic
CGTAATTGATTCGAAAGTGT+TGG 0.488037 3_3:+58496326 None:intergenic
AATAGTTAACCTGACAAGAT+TGG 0.490172 3_3:+58496015 None:intergenic
GTAAATATTTGTTGCCAGCA+AGG 0.508223 3_3:+58496217 None:intergenic
CATCGGTAGGTACCGCAAAA+CGG 0.510977 3_3:+58495504 None:intergenic
TGAATTGTTGGAAACTTTCG+AGG 0.514975 3_3:+58496473 None:intergenic
AACTCACCCTCGGCAAAGGT+TGG 0.517086 3_3:-58495461 Msa0460850:CDS
TCAAAACTCACCCTCGGCAA+AGG 0.527468 3_3:-58495465 Msa0460850:CDS
ACTAAGAGCAGCTAAATTGA+GGG 0.544847 3_3:+58495727 None:intergenic
ATGAATTATAATGAATGCGG+TGG 0.555694 3_3:+58495585 None:intergenic
TTTGCTTCAAAACTCACCCT+CGG 0.556358 3_3:-58495471 Msa0460850:CDS
ATCAACTCTATTCAACTGGC+TGG 0.560237 3_3:-58495777 Msa0460850:CDS
AAGAGCATCTGTACAACGCA+TGG 0.564515 3_3:+58495979 None:intergenic
ACGACAGACAAATTCAAGAC+AGG 0.566363 3_3:-58496645 Msa0460850:CDS
TTTGTCTGTCGTCCTTACGT+TGG 0.566651 3_3:+58496656 None:intergenic
AAAACAAGGGAATGAAGTGT+TGG 0.566708 3_3:+58495908 None:intergenic
TGAGATGAGAAGAAGCATGA+TGG 0.568862 3_3:+58496286 None:intergenic
CATCGACCAATGTGTACTCA+TGG 0.575088 3_3:-58496071 Msa0460850:CDS
CATAGTACTTAATGTAAGAG+TGG 0.576240 3_3:+58496507 None:intergenic
GTGTTGGACATTGTGTGAAA+AGG 0.582799 3_3:+58496342 None:intergenic
AATCACGACCAGTCAAAACA+AGG 0.584573 3_3:+58495894 None:intergenic
CAAAGGTTGGCTTCATCAAG+AGG 0.585363 3_3:-58495448 Msa0460850:CDS
GAGACAGGGTGGTTAATGCT+GGG 0.588135 3_3:+58496173 None:intergenic
AGTATTCAACTTGTTAAATG+CGG 0.588783 3_3:+58496099 None:intergenic
GGCAAGGTGCTTCAGAGACA+GGG 0.593931 3_3:+58496159 None:intergenic
GGAGACACGAAGCCAACGTA+AGG 0.594374 3_3:-58496668 Msa0460850:CDS
TGCGGTACCTACCGATGAGG+TGG 0.594918 3_3:-58495498 Msa0460850:CDS
ATGAAGCCAACCTTTGCCGA+GGG 0.595854 3_3:+58495455 None:intergenic
GATGAAGCCAACCTTTGCCG+AGG 0.606925 3_3:+58495454 None:intergenic
ACTTGTTAAATGCGGAAAAG+GGG 0.607680 3_3:+58496107 None:intergenic
ATCGACCAATGTGTACTCAT+GGG 0.612154 3_3:-58496070 Msa0460850:CDS
ACTTCAAGTTATGCAAGTAA+GGG 0.617231 3_3:+58495549 None:intergenic
TTCTGTGCAAAGAGAGAGTG+TGG 0.622136 3_3:+58496410 None:intergenic
CATCATTACAACAAAAGGCA+AGG 0.625820 3_3:+58496143 None:intergenic
TAAATGAATTATAATGAATG+CGG 0.647864 3_3:+58495582 None:intergenic
TATGTGAATGAGAAGACAGT+CGG 0.648020 3_3:+58496609 None:intergenic
GGATATCAGTTGAGTGATCG+CGG 0.649463 3_3:+58496431 None:intergenic
ATCACGACCAGTCAAAACAA+GGG 0.650578 3_3:+58495895 None:intergenic
GAATTGTTGGAAACTTTCGA+GGG 0.664107 3_3:+58496474 None:intergenic
TATCACCCATGAGTACACAT+TGG 0.703280 3_3:+58496065 None:intergenic
AAGGTGCTTCAGAGACAGGG+TGG 0.729573 3_3:+58496162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCTATAATCTTTACATAT+AGG + chr3_3:58495612-58495631 None:intergenic 15.0%
!! TAAATGAATTATAATGAATG+CGG + chr3_3:58496398-58496417 None:intergenic 15.0%
!! CATTCATTATAATTCATTTA+AGG - chr3_3:58496398-58496417 Msa0460850:CDS 15.0%
!! ATTCTATAATCTTTACATAT+AGG + chr3_3:58495612-58495631 None:intergenic 15.0%
!! TAAATGAATTATAATGAATG+CGG + chr3_3:58496398-58496417 None:intergenic 15.0%
!! CATTCATTATAATTCATTTA+AGG - chr3_3:58496398-58496417 Msa0460850:CDS 15.0%
!!! GATTATATTCCAAAACATTT+TGG - chr3_3:58496102-58496121 Msa0460850:CDS 20.0%
!! ATCTAACATTAAAAAGTAGT+TGG + chr3_3:58496567-58496586 None:intergenic 20.0%
!!! ATGTTAGATATAGATTCTTT+TGG - chr3_3:58496578-58496597 Msa0460850:CDS 20.0%
!!! GATTATATTCCAAAACATTT+TGG - chr3_3:58496102-58496121 Msa0460850:CDS 20.0%
!! ATCTAACATTAAAAAGTAGT+TGG + chr3_3:58496567-58496586 None:intergenic 20.0%
!!! ATGTTAGATATAGATTCTTT+TGG - chr3_3:58496578-58496597 Msa0460850:CDS 20.0%
!! ATTTTATGATTTTTCCTTGC+TGG - chr3_3:58495746-58495765 Msa0460850:CDS 25.0%
!!! AGTATTCAACTTGTTAAATG+CGG + chr3_3:58495881-58495900 None:intergenic 25.0%
! TCCAAAAAAGATTTGGAAAT+GGG + chr3_3:58496034-58496053 None:intergenic 25.0%
! TTCCAAAAAAGATTTGGAAA+TGG + chr3_3:58496035-58496054 None:intergenic 25.0%
!! AAGTTTGTTTCTCTGAATAA+GGG + chr3_3:58496625-58496644 None:intergenic 25.0%
!! ATTTTATGATTTTTCCTTGC+TGG - chr3_3:58495746-58495765 Msa0460850:CDS 25.0%
!!! AGTATTCAACTTGTTAAATG+CGG + chr3_3:58495881-58495900 None:intergenic 25.0%
! TCCAAAAAAGATTTGGAAAT+GGG + chr3_3:58496034-58496053 None:intergenic 25.0%
! TTCCAAAAAAGATTTGGAAA+TGG + chr3_3:58496035-58496054 None:intergenic 25.0%
!! AAGTTTGTTTCTCTGAATAA+GGG + chr3_3:58496625-58496644 None:intergenic 25.0%
! CTTGCATTTTATCCAAAAGA+TGG - chr3_3:58495425-58495444 Msa0460850:three_prime_UTR 30.0%
CATAGTACTTAATGTAAGAG+TGG + chr3_3:58495473-58495492 None:intergenic 30.0%
AAAACGTAGACATGAATTGT+TGG + chr3_3:58495519-58495538 None:intergenic 30.0%
ACCATCATCATTACAACAAA+AGG + chr3_3:58495842-58495861 None:intergenic 30.0%
!! AACTTGTTAAATGCGGAAAA+GGG + chr3_3:58495874-58495893 None:intergenic 30.0%
AATAGTTAACCTGACAAGAT+TGG + chr3_3:58495965-58495984 None:intergenic 30.0%
!! AAAGATTTGGAAATGGGTTT+TGG + chr3_3:58496028-58496047 None:intergenic 30.0%
!!! ACCCATTTCCAAATCTTTTT+TGG - chr3_3:58496030-58496049 Msa0460850:CDS 30.0%
ACTCGATTCCAAAAAAGATT+TGG + chr3_3:58496041-58496060 None:intergenic 30.0%
! TATTGCAGCTTCTATGATTT+TGG - chr3_3:58496167-58496186 Msa0460850:CDS 30.0%
TTGCAAGACATACATATTCT+TGG + chr3_3:58496336-58496355 None:intergenic 30.0%
ATGAATTATAATGAATGCGG+TGG + chr3_3:58496395-58496414 None:intergenic 30.0%
! ACTTCAAGTTATGCAAGTAA+GGG + chr3_3:58496431-58496450 None:intergenic 30.0%
! AACTTCAAGTTATGCAAGTA+AGG + chr3_3:58496432-58496451 None:intergenic 30.0%
CTTGAAGTTGTTGAAATTAG+TGG - chr3_3:58496443-58496462 Msa0460850:CDS 30.0%
! CAAGTTTGTTTCTCTGAATA+AGG + chr3_3:58496626-58496645 None:intergenic 30.0%
! CTTGCATTTTATCCAAAAGA+TGG - chr3_3:58495425-58495444 Msa0460850:three_prime_UTR 30.0%
CATAGTACTTAATGTAAGAG+TGG + chr3_3:58495473-58495492 None:intergenic 30.0%
AAAACGTAGACATGAATTGT+TGG + chr3_3:58495519-58495538 None:intergenic 30.0%
ACCATCATCATTACAACAAA+AGG + chr3_3:58495842-58495861 None:intergenic 30.0%
!! AACTTGTTAAATGCGGAAAA+GGG + chr3_3:58495874-58495893 None:intergenic 30.0%
AATAGTTAACCTGACAAGAT+TGG + chr3_3:58495965-58495984 None:intergenic 30.0%
!! AAAGATTTGGAAATGGGTTT+TGG + chr3_3:58496028-58496047 None:intergenic 30.0%
!!! ACCCATTTCCAAATCTTTTT+TGG - chr3_3:58496030-58496049 Msa0460850:CDS 30.0%
ACTCGATTCCAAAAAAGATT+TGG + chr3_3:58496041-58496060 None:intergenic 30.0%
! TATTGCAGCTTCTATGATTT+TGG - chr3_3:58496167-58496186 Msa0460850:CDS 30.0%
TTGCAAGACATACATATTCT+TGG + chr3_3:58496336-58496355 None:intergenic 30.0%
ATGAATTATAATGAATGCGG+TGG + chr3_3:58496395-58496414 None:intergenic 30.0%
! ACTTCAAGTTATGCAAGTAA+GGG + chr3_3:58496431-58496450 None:intergenic 30.0%
! AACTTCAAGTTATGCAAGTA+AGG + chr3_3:58496432-58496451 None:intergenic 30.0%
CTTGAAGTTGTTGAAATTAG+TGG - chr3_3:58496443-58496462 Msa0460850:CDS 30.0%
! CAAGTTTGTTTCTCTGAATA+AGG + chr3_3:58496626-58496645 None:intergenic 30.0%
TATGTGAATGAGAAGACAGT+CGG + chr3_3:58495371-58495390 None:intergenic 35.0%
GAATTGTTGGAAACTTTCGA+GGG + chr3_3:58495506-58495525 None:intergenic 35.0%
TGAATTGTTGGAAACTTTCG+AGG + chr3_3:58495507-58495526 None:intergenic 35.0%
!! CGTAATTGATTCGAAAGTGT+TGG + chr3_3:58495654-58495673 None:intergenic 35.0%
! GTAAATATTTGTTGCCAGCA+AGG + chr3_3:58495763-58495782 None:intergenic 35.0%
CATCATTACAACAAAAGGCA+AGG + chr3_3:58495837-58495856 None:intergenic 35.0%
!! GCCTTTTGTTGTAATGATGA+TGG - chr3_3:58495838-58495857 Msa0460850:CDS 35.0%
! ACTTGTTAAATGCGGAAAAG+GGG + chr3_3:58495873-58495892 None:intergenic 35.0%
!! CAACTTGTTAAATGCGGAAA+AGG + chr3_3:58495875-58495894 None:intergenic 35.0%
TTTCATGTACCAATCTTGTC+AGG - chr3_3:58495953-58495972 Msa0460850:CDS 35.0%
AAAACAAGGGAATGAAGTGT+TGG + chr3_3:58496072-58496091 None:intergenic 35.0%
! GTCTTGCTTCCAAAATGTTT+TGG + chr3_3:58496114-58496133 None:intergenic 35.0%
ACCAATCAACTCTATTCAAC+TGG - chr3_3:58496196-58496215 Msa0460850:CDS 35.0%
ACTAAGAGCAGCTAAATTGA+GGG + chr3_3:58496253-58496272 None:intergenic 35.0%
!! CTAAGCAATCAGGAAGTATT+AGG + chr3_3:58496364-58496383 None:intergenic 35.0%
CTAATACTTCCTGATTGCTT+AGG - chr3_3:58496362-58496381 Msa0460850:CDS 35.0%
TATGTGAATGAGAAGACAGT+CGG + chr3_3:58495371-58495390 None:intergenic 35.0%
GAATTGTTGGAAACTTTCGA+GGG + chr3_3:58495506-58495525 None:intergenic 35.0%
TGAATTGTTGGAAACTTTCG+AGG + chr3_3:58495507-58495526 None:intergenic 35.0%
!! CGTAATTGATTCGAAAGTGT+TGG + chr3_3:58495654-58495673 None:intergenic 35.0%
! GTAAATATTTGTTGCCAGCA+AGG + chr3_3:58495763-58495782 None:intergenic 35.0%
CATCATTACAACAAAAGGCA+AGG + chr3_3:58495837-58495856 None:intergenic 35.0%
!! GCCTTTTGTTGTAATGATGA+TGG - chr3_3:58495838-58495857 Msa0460850:CDS 35.0%
! ACTTGTTAAATGCGGAAAAG+GGG + chr3_3:58495873-58495892 None:intergenic 35.0%
!! CAACTTGTTAAATGCGGAAA+AGG + chr3_3:58495875-58495894 None:intergenic 35.0%
TTTCATGTACCAATCTTGTC+AGG - chr3_3:58495953-58495972 Msa0460850:CDS 35.0%
AAAACAAGGGAATGAAGTGT+TGG + chr3_3:58496072-58496091 None:intergenic 35.0%
! GTCTTGCTTCCAAAATGTTT+TGG + chr3_3:58496114-58496133 None:intergenic 35.0%
ACCAATCAACTCTATTCAAC+TGG - chr3_3:58496196-58496215 Msa0460850:CDS 35.0%
ACTAAGAGCAGCTAAATTGA+GGG + chr3_3:58496253-58496272 None:intergenic 35.0%
!! CTAAGCAATCAGGAAGTATT+AGG + chr3_3:58496364-58496383 None:intergenic 35.0%
CTAATACTTCCTGATTGCTT+AGG - chr3_3:58496362-58496381 Msa0460850:CDS 35.0%
ACGACAGACAAATTCAAGAC+AGG - chr3_3:58495332-58495351 Msa0460850:three_prime_UTR 40.0%
! CCAGAGATTGATCCATCTTT+TGG + chr3_3:58495440-58495459 None:intergenic 40.0%
CCAAAAGATGGATCAATCTC+TGG - chr3_3:58495437-58495456 Msa0460850:exon 40.0%
GTGTTGGACATTGTGTGAAA+AGG + chr3_3:58495638-58495657 None:intergenic 40.0%
! TGAGATGAGAAGAAGCATGA+TGG + chr3_3:58495694-58495713 None:intergenic 40.0%
ATCGACCAATGTGTACTCAT+GGG - chr3_3:58495907-58495926 Msa0460850:CDS 40.0%
TATCACCCATGAGTACACAT+TGG + chr3_3:58495915-58495934 None:intergenic 40.0%
!! GATTTGGAAATGGGTTTTGG+TGG + chr3_3:58496025-58496044 None:intergenic 40.0%
!!! CTTCATTCCCTTGTTTTGAC+TGG - chr3_3:58496075-58496094 Msa0460850:CDS 40.0%
ATCACGACCAGTCAAAACAA+GGG + chr3_3:58496085-58496104 None:intergenic 40.0%
AATCACGACCAGTCAAAACA+AGG + chr3_3:58496086-58496105 None:intergenic 40.0%
! TGCAGCTTCTATGATTTTGG+TGG - chr3_3:58496170-58496189 Msa0460850:CDS 40.0%
! GCCAGTTGAATAGAGTTGAT+TGG + chr3_3:58496200-58496219 None:intergenic 40.0%
ATCAACTCTATTCAACTGGC+TGG - chr3_3:58496200-58496219 Msa0460850:CDS 40.0%
CACTAAGAGCAGCTAAATTG+AGG + chr3_3:58496254-58496273 None:intergenic 40.0%
AAGCAAAAAGTCCACCTCAT+CGG + chr3_3:58496493-58496512 None:intergenic 40.0%
TTTGCTTCAAAACTCACCCT+CGG - chr3_3:58496506-58496525 Msa0460850:CDS 40.0%
ACGACAGACAAATTCAAGAC+AGG - chr3_3:58495332-58495351 Msa0460850:three_prime_UTR 40.0%
! CCAGAGATTGATCCATCTTT+TGG + chr3_3:58495440-58495459 None:intergenic 40.0%
CCAAAAGATGGATCAATCTC+TGG - chr3_3:58495437-58495456 Msa0460850:exon 40.0%
GTGTTGGACATTGTGTGAAA+AGG + chr3_3:58495638-58495657 None:intergenic 40.0%
! TGAGATGAGAAGAAGCATGA+TGG + chr3_3:58495694-58495713 None:intergenic 40.0%
ATCGACCAATGTGTACTCAT+GGG - chr3_3:58495907-58495926 Msa0460850:CDS 40.0%
TATCACCCATGAGTACACAT+TGG + chr3_3:58495915-58495934 None:intergenic 40.0%
!! GATTTGGAAATGGGTTTTGG+TGG + chr3_3:58496025-58496044 None:intergenic 40.0%
!!! CTTCATTCCCTTGTTTTGAC+TGG - chr3_3:58496075-58496094 Msa0460850:CDS 40.0%
ATCACGACCAGTCAAAACAA+GGG + chr3_3:58496085-58496104 None:intergenic 40.0%
AATCACGACCAGTCAAAACA+AGG + chr3_3:58496086-58496105 None:intergenic 40.0%
! TGCAGCTTCTATGATTTTGG+TGG - chr3_3:58496170-58496189 Msa0460850:CDS 40.0%
! GCCAGTTGAATAGAGTTGAT+TGG + chr3_3:58496200-58496219 None:intergenic 40.0%
ATCAACTCTATTCAACTGGC+TGG - chr3_3:58496200-58496219 Msa0460850:CDS 40.0%
CACTAAGAGCAGCTAAATTG+AGG + chr3_3:58496254-58496273 None:intergenic 40.0%
AAGCAAAAAGTCCACCTCAT+CGG + chr3_3:58496493-58496512 None:intergenic 40.0%
TTTGCTTCAAAACTCACCCT+CGG - chr3_3:58496506-58496525 Msa0460850:CDS 40.0%
TTTGTCTGTCGTCCTTACGT+TGG + chr3_3:58495324-58495343 None:intergenic 45.0%
TCAAGACAGGCTCAGTGAAT+TGG - chr3_3:58495345-58495364 Msa0460850:three_prime_UTR 45.0%
! CTCCTTTTTGAACGCCAAAG+AGG - chr3_3:58495393-58495412 Msa0460850:three_prime_UTR 45.0%
AGCCTCTTTGGCGTTCAAAA+AGG + chr3_3:58495398-58495417 None:intergenic 45.0%
GCAAGTTTGAACAGCCTCTT+TGG + chr3_3:58495410-58495429 None:intergenic 45.0%
GGATATCAGTTGAGTGATCG+CGG + chr3_3:58495549-58495568 None:intergenic 45.0%
TTCTGTGCAAAGAGAGAGTG+TGG + chr3_3:58495570-58495589 None:intergenic 45.0%
TTGACTTCTCTCAGTCTTGC+TGG - chr3_3:58495718-58495737 Msa0460850:intron 45.0%
CATCGACCAATGTGTACTCA+TGG - chr3_3:58495906-58495925 Msa0460850:CDS 45.0%
!! AAGAGCATCTGTACAACGCA+TGG + chr3_3:58496001-58496020 None:intergenic 45.0%
GGACAAGAACCTAAGCAATC+AGG + chr3_3:58496374-58496393 None:intergenic 45.0%
! AGTGGTGTACAACCGTTTTG+CGG - chr3_3:58496461-58496480 Msa0460850:CDS 45.0%
AAAAAGTCCACCTCATCGGT+AGG + chr3_3:58496489-58496508 None:intergenic 45.0%
!! CAAAGGTTGGCTTCATCAAG+AGG - chr3_3:58496529-58496548 Msa0460850:CDS 45.0%
TTTGTCTGTCGTCCTTACGT+TGG + chr3_3:58495324-58495343 None:intergenic 45.0%
TCAAGACAGGCTCAGTGAAT+TGG - chr3_3:58495345-58495364 Msa0460850:three_prime_UTR 45.0%
! CTCCTTTTTGAACGCCAAAG+AGG - chr3_3:58495393-58495412 Msa0460850:three_prime_UTR 45.0%
AGCCTCTTTGGCGTTCAAAA+AGG + chr3_3:58495398-58495417 None:intergenic 45.0%
GCAAGTTTGAACAGCCTCTT+TGG + chr3_3:58495410-58495429 None:intergenic 45.0%
GGATATCAGTTGAGTGATCG+CGG + chr3_3:58495549-58495568 None:intergenic 45.0%
TTCTGTGCAAAGAGAGAGTG+TGG + chr3_3:58495570-58495589 None:intergenic 45.0%
TTGACTTCTCTCAGTCTTGC+TGG - chr3_3:58495718-58495737 Msa0460850:intron 45.0%
CATCGACCAATGTGTACTCA+TGG - chr3_3:58495906-58495925 Msa0460850:CDS 45.0%
!! AAGAGCATCTGTACAACGCA+TGG + chr3_3:58496001-58496020 None:intergenic 45.0%
GGACAAGAACCTAAGCAATC+AGG + chr3_3:58496374-58496393 None:intergenic 45.0%
! AGTGGTGTACAACCGTTTTG+CGG - chr3_3:58496461-58496480 Msa0460850:CDS 45.0%
AAAAAGTCCACCTCATCGGT+AGG + chr3_3:58496489-58496508 None:intergenic 45.0%
!! CAAAGGTTGGCTTCATCAAG+AGG - chr3_3:58496529-58496548 Msa0460850:CDS 45.0%
GAGACAGGGTGGTTAATGCT+GGG + chr3_3:58495807-58495826 None:intergenic 50.0%
AGAGACAGGGTGGTTAATGC+TGG + chr3_3:58495808-58495827 None:intergenic 50.0%
! CTCTATTCAACTGGCTGGCT+TGG - chr3_3:58496205-58496224 Msa0460850:CDS 50.0%
! CATCGGTAGGTACCGCAAAA+CGG + chr3_3:58496476-58496495 None:intergenic 50.0%
! TTTTGCGGTACCTACCGATG+AGG - chr3_3:58496476-58496495 Msa0460850:CDS 50.0%
TCAAAACTCACCCTCGGCAA+AGG - chr3_3:58496512-58496531 Msa0460850:CDS 50.0%
ATGAAGCCAACCTTTGCCGA+GGG + chr3_3:58496525-58496544 None:intergenic 50.0%
GAGACAGGGTGGTTAATGCT+GGG + chr3_3:58495807-58495826 None:intergenic 50.0%
AGAGACAGGGTGGTTAATGC+TGG + chr3_3:58495808-58495827 None:intergenic 50.0%
! CTCTATTCAACTGGCTGGCT+TGG - chr3_3:58496205-58496224 Msa0460850:CDS 50.0%
! CATCGGTAGGTACCGCAAAA+CGG + chr3_3:58496476-58496495 None:intergenic 50.0%
! TTTTGCGGTACCTACCGATG+AGG - chr3_3:58496476-58496495 Msa0460850:CDS 50.0%
TCAAAACTCACCCTCGGCAA+AGG - chr3_3:58496512-58496531 Msa0460850:CDS 50.0%
ATGAAGCCAACCTTTGCCGA+GGG + chr3_3:58496525-58496544 None:intergenic 50.0%
GGAGACACGAAGCCAACGTA+AGG - chr3_3:58495309-58495328 Msa0460850:three_prime_UTR 55.0%
! AAGGTGCTTCAGAGACAGGG+TGG + chr3_3:58495818-58495837 None:intergenic 55.0%
! GGCAAGGTGCTTCAGAGACA+GGG + chr3_3:58495821-58495840 None:intergenic 55.0%
! AGGCAAGGTGCTTCAGAGAC+AGG + chr3_3:58495822-58495841 None:intergenic 55.0%
AACTCACCCTCGGCAAAGGT+TGG - chr3_3:58496516-58496535 Msa0460850:CDS 55.0%
GATGAAGCCAACCTTTGCCG+AGG + chr3_3:58496526-58496545 None:intergenic 55.0%
GGAGACACGAAGCCAACGTA+AGG - chr3_3:58495309-58495328 Msa0460850:three_prime_UTR 55.0%
! AAGGTGCTTCAGAGACAGGG+TGG + chr3_3:58495818-58495837 None:intergenic 55.0%
! GGCAAGGTGCTTCAGAGACA+GGG + chr3_3:58495821-58495840 None:intergenic 55.0%
! AGGCAAGGTGCTTCAGAGAC+AGG + chr3_3:58495822-58495841 None:intergenic 55.0%
AACTCACCCTCGGCAAAGGT+TGG - chr3_3:58496516-58496535 Msa0460850:CDS 55.0%
GATGAAGCCAACCTTTGCCG+AGG + chr3_3:58496526-58496545 None:intergenic 55.0%
TGCGGTACCTACCGATGAGG+TGG - chr3_3:58496479-58496498 Msa0460850:CDS 60.0%
TGCGGTACCTACCGATGAGG+TGG - chr3_3:58496479-58496498 Msa0460850:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 58495304 58496695 58495304 ID=Msa0460850;Name=Msa0460850
chr3_3 mRNA 58495304 58496695 58495304 ID=Msa0460850-mRNA-1;Parent=Msa0460850;Name=Msa0460850-mRNA-1;_AED=0.19;_eAED=0.20;_QI=0|1|0.5|1|1|1|2|141|379
chr3_3 exon 58495304 58495615 58495304 ID=Msa0460850-mRNA-1:exon:12164;Parent=Msa0460850-mRNA-1
chr3_3 exon 58495727 58496695 58495727 ID=Msa0460850-mRNA-1:exon:12163;Parent=Msa0460850-mRNA-1
chr3_3 CDS 58495727 58496695 58495727 ID=Msa0460850-mRNA-1:cds;Parent=Msa0460850-mRNA-1
chr3_3 CDS 58495445 58495615 58495445 ID=Msa0460850-mRNA-1:cds;Parent=Msa0460850-mRNA-1
chr3_3 three_prime_UTR 58495304 58495444 58495304 ID=Msa0460850-mRNA-1:three_prime_utr;Parent=Msa0460850-mRNA-1
Gene Sequence

>Msa0460850

ATGGTGGAGACACGAAGCCAACGTAAGGACGACAGACAAATTCAAGACAGGCTCAGTGAATTGGCCGACTGTCTTCTCATTCACATACTCTCCTTTTTGAACGCCAAAGAGGCTGTTCAAACTTGCATTTTATCCAAAAGATGGATCAATCTCTGGAAGACTCTTTCCACTCTTACATTAAGTACTATGCAATTTAGTACCCTCGAAAGTTTCCAACAATTCATGTCTACGTTTTTGTCTCTCCGCGATCACTCAACTGATATCCACACTCTCTCTTTGCACAGAAGTCATTTTGTCAGTCATGACCTATATGTAAAGATTATAGAATACGCCTTTTCACACAATGTCCAACACTTTCGAATCAATTACGCTACTATTCAACACTTTCCATCATGCTTCTTCTCATCTCACACTTTGACTTCTCTCAGTCTTGCTGGTGTCAATTTTATGATTTTTCCTTGCTGGCAACAAATATTTACAGATTCTCGTTCTTTCAATTTCCCAGCATTAACCACCCTGTCTCTGAAGCACCTTGCCTTTTGTTGTAATGATGATGGTTGTGTCGACCCCTTTTCCGCATTTAACAAGTTGAATACTTTGATCATCGACCAATGTGTACTCATGGGTGATAATGCAAAAAAACTTCGCATTTCATGTACCAATCTTGTCAGGTTAACTATTCATATGTATTATTCCATGCGTTGTACAGATGCTCTTACCACCAAAACCCATTTCCAAATCTTTTTTGGAATCGAGTTAAATGCTCCAACACTTCATTCCCTTGTTTTGACTGGTCGTGATTATATTCCAAAACATTTTGGAAGCAAGACTCTTCTCTCTTCTATCAAACATCTAAATATTCATATTGCAGCTTCTATGATTTTGGTGGAGAACCAATCAACTCTATTCAACTGGCTGGCTTGGCTCTCTAATATCGAATCATTCACCCTCAATTTAGCTGCTCTTAGTGTTCTTGTCCACCGCATTCATTATAATTCATTTAAGGATAAGTTCCCTTACTTGCATAACTTGAAGTTGTTGAAATTAGTGGTGTACAACCGTTTTGCGGTACCTACCGATGAGGTGGACTTTTTGCTTCAAAACTCACCCTCGGCAAAGGTTGGCTTCATCAAGAGGTAA
Protein sequence

>Msa0460850

MVETRSQRKDDRQIQDRLSELADCLLIHILSFLNAKEAVQTCILSKRWINLWKTLSTLTLSTMQFSTLESFQQFMSTFLSLRDHSTDIHTLSLHRSHFVSHDLYVKIIEYAFSHNVQHFRINYATIQHFPSCFFSSHTLTSLSLAGVNFMIFPCWQQIFTDSRSFNFPALTTLSLKHLAFCCNDDGCVDPFSAFNKLNTLIIDQCVLMGDNAKKLRISCTNLVRLTIHMYYSMRCTDALTTKTHFQIFFGIELNAPTLHSLVLTGRDYIPKHFGSKTLLSSIKHLNIHIAASMILVENQSTLFNWLAWLSNIESFTLNLAALSVLVHRIHYNSFKDKFPYLHNLKLLKLVVYNRFAVPTDEVDFLLQNSPSAKVGFIKR*