AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0465750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0465750 MtrunA17_Chr3g0116271 99.492 197 1 0 1 197 366 562 4.08e-143 410
Msa0465750 MtrunA17_Chr3g0116251 97.970 197 4 0 1 197 364 560 2.73e-140 402
Msa0465750 MtrunA17_Chr1g0186071 63.636 187 67 1 1 187 365 550 4.52e-88 269
Msa0465750 MtrunA17_Chr1g0157401 61.290 186 72 0 4 189 371 556 3.03e-84 259
Msa0465750 MtrunA17_Chr5g0409771 56.757 185 78 1 3 185 363 547 2.98e-71 225
Msa0465750 MtrunA17_Chr4g0039791 55.676 185 80 1 3 185 369 553 7.67e-70 222
Msa0465750 MtrunA17_Chr2g0327341 54.945 182 81 1 9 189 354 535 2.09e-69 220
Msa0465750 MtrunA17_Chr4g0028411 55.866 179 78 1 9 186 357 535 2.06e-68 218
Msa0465750 MtrunA17_Chr5g0409791 55.208 192 83 2 3 192 376 566 2.50e-68 218
Msa0465750 MtrunA17_Chr1g0197391 58.242 182 75 1 6 186 378 559 9.61e-68 216
Msa0465750 MtrunA17_Chr2g0334131 56.180 178 77 1 9 185 99 276 1.13e-65 203
Msa0465750 MtrunA17_Chr8g0353341 62.092 153 58 0 4 156 374 526 1.67e-65 210
Msa0465750 MtrunA17_Chr1g0180551 52.604 192 85 1 1 186 423 614 1.75e-65 212
Msa0465750 MtrunA17_Chr1g0180541 53.371 178 83 0 11 188 366 543 4.99e-64 206
Msa0465750 MtrunA17_Chr5g0395431 55.429 175 78 0 11 185 405 579 7.08e-64 207
Msa0465750 MtrunA17_Chr4g0072561 55.429 175 78 0 11 185 366 540 2.89e-56 186
Msa0465750 MtrunA17_Chr4g0000461 55.618 178 78 1 9 185 357 534 9.71e-56 184
Msa0465750 MtrunA17_Chr3g0102411 50.289 173 86 0 11 183 359 531 4.66e-54 180
Msa0465750 MtrunA17_Chr6g0471921 35.196 179 113 3 5 183 330 505 6.70e-29 112
Msa0465750 MtrunA17_Chr4g0028451 45.794 107 49 1 51 157 10 107 1.30e-27 100
Msa0465750 MtrunA17_Chr5g0396311 35.751 193 119 4 5 194 351 541 9.04e-27 106
Msa0465750 MtrunA17_Chr8g0370701 36.364 176 108 4 21 194 412 585 1.34e-25 103
Msa0465750 MtrunA17_Chr8g0370731 34.659 176 111 3 21 194 323 496 9.15e-24 97.8
Msa0465750 MtrunA17_Chr6g0471311 31.694 183 118 3 21 197 374 555 1.99e-23 97.1
Msa0465750 MtrunA17_Chr5g0396331 32.955 176 114 3 21 194 366 539 2.92e-22 93.6
Msa0465750 MtrunA17_Chr3g0109731 32.571 175 102 4 18 185 381 546 7.76e-22 92.4
Msa0465750 MtrunA17_Chr5g0433231 32.222 180 115 3 21 194 372 550 7.84e-21 89.4
Msa0465750 MtrunA17_Chr2g0301671 34.756 164 100 4 39 197 402 563 9.35e-21 89.4
Msa0465750 MtrunA17_Chr4g0068151 34.211 152 93 3 39 185 402 551 2.40e-20 88.2
Msa0465750 MtrunA17_Chr5g0433221 31.148 183 119 4 21 197 372 553 3.45e-20 87.4
Msa0465750 MtrunA17_Chr5g0433241 34.839 155 96 2 39 189 391 544 7.58e-20 86.7
Msa0465750 MtrunA17_Chr4g0004101 26.486 185 128 3 8 185 140 323 6.05e-19 82.8
Msa0465750 MtrunA17_Chr4g0004841 27.179 195 124 5 8 188 145 335 1.89e-17 79.0
Msa0465750 MtrunA17_Chr8g0377991 42.056 107 51 2 11 106 502 608 1.54e-15 74.3
Msa0465750 MtrunA17_Chr3g0121931 41.121 107 52 2 11 106 499 605 1.01e-14 72.0
Msa0465750 MtrunA17_Chr3g0121951 39.252 107 54 2 11 106 495 601 4.09e-14 70.1
Msa0465750 MtrunA17_Chr7g0252691 32.540 126 82 1 67 189 598 723 3.57e-12 64.3
Msa0465750 MtrunA17_Chr1g0205711 36.275 102 61 2 11 108 465 566 1.10e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0465750 AT5G63380.1 74.490 196 50 0 2 197 367 562 5.18e-109 323
Msa0465750 AT1G20510.1 62.176 193 71 1 5 197 356 546 6.84e-90 273
Msa0465750 AT1G20500.1 62.162 185 70 0 5 189 362 546 6.38e-84 258
Msa0465750 AT1G20480.1 62.766 188 70 0 1 188 373 560 2.12e-83 257
Msa0465750 AT5G38120.1 59.893 187 72 1 5 191 365 548 2.69e-81 251
Msa0465750 AT4G05160.1 58.101 179 75 0 8 186 359 537 4.56e-73 230
Msa0465750 AT4G19010.1 54.315 197 88 1 3 197 370 566 4.39e-72 228
Msa0465750 AT5G38120.3 60.131 153 58 1 5 157 365 514 6.23e-64 206
Msa0465750 AT3G21240.1 54.237 177 81 0 9 185 370 546 4.22e-61 199
Msa0465750 AT1G65060.1 55.367 177 79 0 9 185 380 556 1.92e-59 195
Msa0465750 AT3G21230.1 51.977 177 85 0 9 185 384 560 1.11e-55 185
Msa0465750 AT1G20510.2 66.102 118 40 0 5 122 356 473 6.30e-55 181
Msa0465750 AT1G51680.1 53.672 177 82 0 9 185 377 553 4.82e-54 181
Msa0465750 AT1G62940.1 51.892 185 88 1 7 191 355 538 6.39e-51 172
Msa0465750 AT5G38120.4 61.864 118 42 1 5 122 365 479 9.42e-47 160
Msa0465750 AT5G38120.2 61.864 118 42 1 5 122 365 479 9.42e-47 160
Msa0465750 AT3G21230.3 51.678 149 72 0 9 157 384 532 1.44e-44 155
Msa0465750 AT1G20510.3 65.625 96 33 0 5 100 356 451 1.65e-43 151
Msa0465750 AT1G51680.3 53.020 149 70 0 9 157 377 525 1.21e-41 147
Msa0465750 AT3G21240.2 55.652 115 51 0 9 123 370 484 3.08e-36 132
Msa0465750 AT3G21240.3 55.652 115 51 0 9 123 370 484 3.08e-36 132
Msa0465750 AT3G21230.2 53.097 113 53 0 9 121 384 496 1.63e-33 125
Msa0465750 AT1G51680.2 57.522 113 48 0 9 121 377 489 4.85e-32 120
Msa0465750 AT3G48990.1 34.682 173 110 3 11 183 335 504 9.38e-29 112
Msa0465750 AT5G16370.1 37.017 181 109 3 21 197 370 549 1.70e-28 111
Msa0465750 AT1G75960.1 38.728 173 101 3 21 189 370 541 7.99e-28 109
Msa0465750 AT5G16340.1 38.728 173 101 3 21 189 370 541 6.39e-27 107
Msa0465750 AT1G77240.1 32.804 189 123 3 5 189 353 541 3.93e-26 105
Msa0465750 AT1G21540.1 34.286 175 109 2 21 189 372 546 8.33e-25 101
Msa0465750 AT1G21530.2 33.520 179 111 2 21 191 369 547 4.15e-24 99.4
Msa0465750 AT1G21530.1 33.520 179 111 2 21 191 367 545 4.46e-24 99.0
Msa0465750 AT1G65060.2 50.000 94 47 0 9 102 380 473 3.14e-23 96.7
Msa0465750 AT1G66120.1 35.714 154 95 2 39 189 393 545 7.46e-23 95.5
Msa0465750 AT1G65880.1 32.787 183 106 4 18 189 377 553 4.71e-22 93.6
Msa0465750 AT1G68270.1 33.333 165 97 3 39 193 363 524 8.00e-22 92.8
Msa0465750 AT1G65890.1 33.540 161 96 3 39 189 393 552 1.36e-21 92.0
Msa0465750 AT1G20560.2 34.756 164 103 3 21 182 296 457 1.46e-21 91.7
Msa0465750 AT1G20560.1 35.185 162 101 3 21 180 374 533 1.80e-21 91.7
Msa0465750 AT2G17650.1 32.184 174 113 4 24 194 431 602 4.31e-18 82.0
Msa0465750 AT1G20490.2 53.030 66 31 0 5 70 364 429 9.81e-18 80.9
Msa0465750 AT1G20490.1 53.030 66 31 0 5 70 364 429 9.81e-18 80.9
Msa0465750 AT1G76290.1 32.022 178 116 3 24 197 366 542 1.91e-17 80.1
Msa0465750 AT3G16910.1 30.319 188 121 5 6 185 369 554 6.30e-17 78.6
Msa0465750 AT3G16170.1 27.368 190 123 4 7 185 415 600 1.41e-16 77.8
Msa0465750 AT5G36880.6 32.540 126 82 1 67 189 540 665 5.09e-12 64.3
Msa0465750 AT5G36880.3 32.540 126 82 1 67 189 540 665 5.09e-12 64.3
Msa0465750 AT5G36880.5 32.540 126 82 1 67 189 540 665 5.09e-12 64.3
Msa0465750 AT5G36880.1 32.540 126 82 1 67 189 540 665 5.09e-12 64.3
Msa0465750 AT5G36880.2 32.540 126 82 1 67 189 590 715 5.41e-12 64.3
Msa0465750 AT3G23790.1 48.438 64 33 0 11 74 500 563 1.04e-11 63.5
Msa0465750 AT5G27600.1 37.255 102 60 2 11 108 468 569 2.61e-11 62.4
Msa0465750 AT4G14070.1 46.032 63 34 0 11 73 516 578 6.28e-11 61.2

Find 62 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACCAAAGCTCCCCTTTC+TGG 0.150099 3_3:-66202450 None:intergenic
ACTGCAGCATCAGCAATTTC+AGG 0.286327 3_3:-66203140 None:intergenic
ACCTTTATCAGCTATTCTTT+TGG 0.288642 3_3:+66204031 Msa0465750:three_prime_UTR
GCTGCAGTGATGTTGCTTCC+TGG 0.300397 3_3:-66203382 None:intergenic
GATCAAATACAAAGCTTATC+AGG 0.313622 3_3:+66202997 Msa0465750:CDS
AAGATAGTTGACCCTGTTAC+TGG 0.325199 3_3:+66202409 Msa0465750:CDS
GATAATGACTCCCCAGTAAC+AGG 0.332219 3_3:-66202421 None:intergenic
TAATTACCTTTCATGATTGT+TGG 0.332934 3_3:-66202478 None:intergenic
CATTTAATCATTTATTCATA+AGG 0.346497 3_3:+66203967 Msa0465750:three_prime_UTR
CTGGCCAGAAAGGGGAGCTT+TGG 0.355601 3_3:+66202446 Msa0465750:CDS
TTGTGCTTGTTAGTAATTAG+TGG 0.359654 3_3:+66203994 Msa0465750:three_prime_UTR
ATTGTTTACTTGTAGGGATA+TGG 0.368434 3_3:+66202289 None:intergenic
GCCAAAAGAATAGCTGATAA+AGG 0.385076 3_3:-66204032 None:intergenic
GGGTCGACTAGCAGAAAATA+TGG 0.400111 3_3:+66202381 Msa0465750:CDS
CATATAGGAGTATAACAATA+TGG 0.410278 3_3:-66203930 None:intergenic
GGAGGGGCAGCAAGAATGAT+AGG 0.430991 3_3:+66202328 Msa0465750:exon
ATTTGCCCTGCATCTTCATC+AGG 0.445963 3_3:-66203334 None:intergenic
CATTATCTCCTGGCCAGAAA+GGG 0.448093 3_3:+66202437 Msa0465750:CDS
CAAAGCTCCCCTTTCTGGCC+AGG 0.450934 3_3:-66202445 None:intergenic
CATGGACTATGTTGCGAAGC+AGG 0.450950 3_3:+66203411 Msa0465750:CDS
GAATAAATGATTAAATGTAT+TGG 0.462580 3_3:-66203961 None:intergenic
AAGCAACATCACTGCAGCTC+AGG 0.475913 3_3:+66203387 Msa0465750:CDS
AGTATGTGCTCTAATTCAGC+TGG 0.482576 3_3:-66203107 None:intergenic
AGCATCAGCAATTTCAGGAT+TGG 0.493174 3_3:-66203135 None:intergenic
ACTGGGGAGTCATTATCTCC+TGG 0.514409 3_3:+66202427 Msa0465750:CDS
GGATATGGTCTGACTGAAAG+TGG 0.522524 3_3:+66202304 Msa0465750:five_prime_UTR
GTTGATATTGCTCTAACTAG+TGG 0.525743 3_3:+66203839 Msa0465750:CDS
AGATAGTTGACCCTGTTACT+GGG 0.529008 3_3:+66202410 Msa0465750:CDS
CAAGGCGACTGTTGAGACAT+TGG 0.529632 3_3:+66202889 Msa0465750:CDS
GGTGCCAGTGCAGGTTATGT+AGG 0.531010 3_3:+66202860 Msa0465750:intron
AGCTAAGCGCCACGGTTCTG+TGG 0.533413 3_3:+66202360 Msa0465750:CDS
AGGTATCCTGATGAAGATGC+AGG 0.534795 3_3:+66203328 Msa0465750:intron
TCATTATCTCCTGGCCAGAA+AGG 0.535246 3_3:+66202436 Msa0465750:CDS
GGTCTGACTGAAAGTGGAGG+AGG 0.537107 3_3:+66202310 Msa0465750:five_prime_UTR
TTTCTTACCACAAAAGCCAT+TGG 0.538521 3_3:-66203358 None:intergenic
CATCACTGCAGCTCAGGTCA+TGG 0.542708 3_3:+66203393 Msa0465750:CDS
GCTAAGCGCCACGGTTCTGT+GGG 0.543305 3_3:+66202361 Msa0465750:CDS
GTCTGACTGAAAGTGGAGGA+GGG 0.551229 3_3:+66202311 Msa0465750:five_prime_UTR
AAAGGGGAGCTTTGGTTGCG+AGG 0.553296 3_3:+66202454 Msa0465750:CDS
TCAGAGGGATGGCTGAAGAC+CGG 0.555043 3_3:+66202914 Msa0465750:CDS
TGTTGAGACATTGGATTCAG+AGG 0.562613 3_3:+66202898 Msa0465750:CDS
GTATTGGTGAAGTATCATAT+AGG 0.569161 3_3:-66203945 None:intergenic
TCTATTCCAAAATCTCCAGC+TGG 0.577274 3_3:+66203794 Msa0465750:CDS
GTTGAGACATTGGATTCAGA+GGG 0.583163 3_3:+66202899 Msa0465750:CDS
GTATGTGCTCTAATTCAGCT+GGG 0.588117 3_3:-66203106 None:intergenic
GTCAAAATAACAAAGATCAC+CGG 0.592946 3_3:-66202933 None:intergenic
GATAGTTGACCCTGTTACTG+GGG 0.593991 3_3:+66202411 Msa0465750:CDS
AGACATTGGATTCAGAGGGA+TGG 0.601070 3_3:+66202903 Msa0465750:CDS
ACACGTCGTATCTTCTTGTA+CGG 0.603152 3_3:-66203758 None:intergenic
GTTCATTGTAGATAGACTGA+AGG 0.608097 3_3:+66202970 Msa0465750:CDS
ATTATCTCCTGGCCAGAAAG+GGG 0.612166 3_3:+66202438 Msa0465750:CDS
TTTGATGAAGCTAAGCGCCA+CGG 0.614262 3_3:+66202352 Msa0465750:CDS
TCTGACTGAAAGTGGAGGAG+GGG 0.617119 3_3:+66202312 Msa0465750:five_prime_UTR
CGAGGACCAACAATCATGAA+AGG 0.618566 3_3:+66202472 Msa0465750:CDS
ATGTGCTCTAATTCAGCTGG+GGG 0.639820 3_3:-66203104 None:intergenic
AGATGCAGGGCAAATTCCAA+TGG 0.641785 3_3:+66203342 Msa0465750:CDS
GGTATCCTGATGAAGATGCA+GGG 0.646637 3_3:+66203329 Msa0465750:intron
TATGTGCTCTAATTCAGCTG+GGG 0.654273 3_3:-66203105 None:intergenic
ATAATGACTCCCCAGTAACA+GGG 0.657918 3_3:-66202420 None:intergenic
AGGTTATGTAGGAGATGACA+AGG 0.664375 3_3:+66202871 Msa0465750:intron
TATGGTCTGACTGAAAGTGG+AGG 0.699989 3_3:+66202307 Msa0465750:five_prime_UTR
CTAGTCGACCCACAGAACCG+TGG 0.717085 3_3:-66202369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATACTAGTTTTTTTTTT+AGG + chr3_3:66204107-66204126 Msa0465750:three_prime_UTR 10.0%
!! TTAAGTAAAGATAATATTAG+TGG + chr3_3:66202651-66202670 Msa0465750:intron 15.0%
!!! TAAATCTAATACTAATCTTT+TGG + chr3_3:66203300-66203319 Msa0465750:intron 15.0%
!!! AAATCTAATACTAATCTTTT+GGG + chr3_3:66203301-66203320 Msa0465750:intron 15.0%
!! GAATAAATGATTAAATGTAT+TGG - chr3_3:66203964-66203983 None:intergenic 15.0%
!! CATTTAATCATTTATTCATA+AGG + chr3_3:66203967-66203986 Msa0465750:three_prime_UTR 15.0%
!!! AAAAAAAACTAGTATTTTAC+AGG - chr3_3:66204105-66204124 None:intergenic 15.0%
!! TTAAGTAAAGATAATATTAG+TGG + chr3_3:66202651-66202670 Msa0465750:intron 15.0%
!!! TAAATCTAATACTAATCTTT+TGG + chr3_3:66203300-66203319 Msa0465750:intron 15.0%
!!! AAATCTAATACTAATCTTTT+GGG + chr3_3:66203301-66203320 Msa0465750:intron 15.0%
!! GAATAAATGATTAAATGTAT+TGG - chr3_3:66203964-66203983 None:intergenic 15.0%
!! CATTTAATCATTTATTCATA+AGG + chr3_3:66203967-66203986 Msa0465750:three_prime_UTR 15.0%
!!! AAAAAAAACTAGTATTTTAC+AGG - chr3_3:66204105-66204124 None:intergenic 15.0%
!! TAATAAGGAAATGTAAAGAT+AGG - chr3_3:66203073-66203092 None:intergenic 20.0%
!!! ACATTTCCTTATTATTTTTC+AGG + chr3_3:66203079-66203098 Msa0465750:intron 20.0%
!!! AATCTAATACTAATCTTTTG+GGG + chr3_3:66203302-66203321 Msa0465750:intron 20.0%
!! TTAAAACTTCAACAAAATCA+TGG - chr3_3:66203546-66203565 None:intergenic 20.0%
!! GTAATTTATCCAAAATATAG+CGG + chr3_3:66203569-66203588 Msa0465750:intron 20.0%
!! TAATAAGGAAATGTAAAGAT+AGG - chr3_3:66203073-66203092 None:intergenic 20.0%
!!! ACATTTCCTTATTATTTTTC+AGG + chr3_3:66203079-66203098 Msa0465750:intron 20.0%
!!! AATCTAATACTAATCTTTTG+GGG + chr3_3:66203302-66203321 Msa0465750:intron 20.0%
!! TTAAAACTTCAACAAAATCA+TGG - chr3_3:66203546-66203565 None:intergenic 20.0%
!! GTAATTTATCCAAAATATAG+CGG + chr3_3:66203569-66203588 Msa0465750:intron 20.0%
! TAATTACCTTTCATGATTGT+TGG - chr3_3:66202481-66202500 None:intergenic 25.0%
! TTAATAAACCTCTCCATTTA+TGG + chr3_3:66202498-66202517 Msa0465750:intron 25.0%
! TAATAAACCTCTCCATTTAT+GGG + chr3_3:66202499-66202518 Msa0465750:intron 25.0%
! AGCAATCATATGTAATAGAT+GGG - chr3_3:66202754-66202773 None:intergenic 25.0%
! TAGCAATCATATGTAATAGA+TGG - chr3_3:66202755-66202774 None:intergenic 25.0%
! GTACAGCTTAACAAAAAAAT+TGG - chr3_3:66202788-66202807 None:intergenic 25.0%
! AAGATATCTCAAGCTATATT+TGG - chr3_3:66202835-66202854 None:intergenic 25.0%
! TAGCTTGAGATATCTTATTA+TGG + chr3_3:66202839-66202858 Msa0465750:intron 25.0%
! ATTAAAGCAGTAGATACATA+CGG - chr3_3:66203170-66203189 None:intergenic 25.0%
!! CATATAGGAGTATAACAATA+TGG - chr3_3:66203933-66203952 None:intergenic 25.0%
! TAATTACCTTTCATGATTGT+TGG - chr3_3:66202481-66202500 None:intergenic 25.0%
! TTAATAAACCTCTCCATTTA+TGG + chr3_3:66202498-66202517 Msa0465750:intron 25.0%
! TAATAAACCTCTCCATTTAT+GGG + chr3_3:66202499-66202518 Msa0465750:intron 25.0%
! AGCAATCATATGTAATAGAT+GGG - chr3_3:66202754-66202773 None:intergenic 25.0%
! TAGCAATCATATGTAATAGA+TGG - chr3_3:66202755-66202774 None:intergenic 25.0%
! GTACAGCTTAACAAAAAAAT+TGG - chr3_3:66202788-66202807 None:intergenic 25.0%
! AAGATATCTCAAGCTATATT+TGG - chr3_3:66202835-66202854 None:intergenic 25.0%
! TAGCTTGAGATATCTTATTA+TGG + chr3_3:66202839-66202858 Msa0465750:intron 25.0%
! ATTAAAGCAGTAGATACATA+CGG - chr3_3:66203170-66203189 None:intergenic 25.0%
!! CATATAGGAGTATAACAATA+TGG - chr3_3:66203933-66203952 None:intergenic 25.0%
CTAACATTATGCAAATGCAA+TGG - chr3_3:66202527-66202546 None:intergenic 30.0%
CATTGCATTTGCATAATGTT+AGG + chr3_3:66202525-66202544 Msa0465750:intron 30.0%
TCATGAACATAGCAAATCTT+TGG - chr3_3:66202573-66202592 None:intergenic 30.0%
! TCTCACTTTAGTTCATTGAT+TGG + chr3_3:66202702-66202721 Msa0465750:intron 30.0%
GTCAAAATAACAAAGATCAC+CGG - chr3_3:66202936-66202955 None:intergenic 30.0%
!!! CTTTGTTATTTTGACTCAGA+TGG + chr3_3:66202941-66202960 Msa0465750:CDS 30.0%
GATCAAATACAAAGCTTATC+AGG + chr3_3:66202997-66203016 Msa0465750:CDS 30.0%
TTAAAGCAGTAGATACATAC+GGG - chr3_3:66203169-66203188 None:intergenic 30.0%
TACATGACTGTCTAATAAGA+AGG + chr3_3:66203272-66203291 Msa0465750:intron 30.0%
!! TGTTCTAAAATGCAGATATG+TGG + chr3_3:66203436-66203455 Msa0465750:intron 30.0%
CAAGAGTTAAGAAAATCCAA+CGG - chr3_3:66203476-66203495 None:intergenic 30.0%
ATCATGTTCATTATTGTTGC+AGG + chr3_3:66203730-66203749 Msa0465750:intron 30.0%
! GTATTGGTGAAGTATCATAT+AGG - chr3_3:66203948-66203967 None:intergenic 30.0%
! TTGTGCTTGTTAGTAATTAG+TGG + chr3_3:66203994-66204013 Msa0465750:three_prime_UTR 30.0%
! ACCTTTATCAGCTATTCTTT+TGG + chr3_3:66204031-66204050 Msa0465750:three_prime_UTR 30.0%
CTAACATTATGCAAATGCAA+TGG - chr3_3:66202527-66202546 None:intergenic 30.0%
CATTGCATTTGCATAATGTT+AGG + chr3_3:66202525-66202544 Msa0465750:intron 30.0%
TCATGAACATAGCAAATCTT+TGG - chr3_3:66202573-66202592 None:intergenic 30.0%
! TCTCACTTTAGTTCATTGAT+TGG + chr3_3:66202702-66202721 Msa0465750:intron 30.0%
GTCAAAATAACAAAGATCAC+CGG - chr3_3:66202936-66202955 None:intergenic 30.0%
!!! CTTTGTTATTTTGACTCAGA+TGG + chr3_3:66202941-66202960 Msa0465750:CDS 30.0%
GATCAAATACAAAGCTTATC+AGG + chr3_3:66202997-66203016 Msa0465750:CDS 30.0%
TTAAAGCAGTAGATACATAC+GGG - chr3_3:66203169-66203188 None:intergenic 30.0%
TACATGACTGTCTAATAAGA+AGG + chr3_3:66203272-66203291 Msa0465750:intron 30.0%
!! TGTTCTAAAATGCAGATATG+TGG + chr3_3:66203436-66203455 Msa0465750:intron 30.0%
CAAGAGTTAAGAAAATCCAA+CGG - chr3_3:66203476-66203495 None:intergenic 30.0%
ATCATGTTCATTATTGTTGC+AGG + chr3_3:66203730-66203749 Msa0465750:intron 30.0%
! GTATTGGTGAAGTATCATAT+AGG - chr3_3:66203948-66203967 None:intergenic 30.0%
! TTGTGCTTGTTAGTAATTAG+TGG + chr3_3:66203994-66204013 Msa0465750:three_prime_UTR 30.0%
! ACCTTTATCAGCTATTCTTT+TGG + chr3_3:66204031-66204050 Msa0465750:three_prime_UTR 30.0%
AATGCAATGGTTCCCATAAA+TGG - chr3_3:66202514-66202533 None:intergenic 35.0%
GTTCATTGTAGATAGACTGA+AGG + chr3_3:66202970-66202989 Msa0465750:CDS 35.0%
TAAAGATAGGCGATAGCAAA+TGG - chr3_3:66203060-66203079 None:intergenic 35.0%
GGGGAACCTGAAAAATAATA+AGG - chr3_3:66203088-66203107 None:intergenic 35.0%
TTTCTTACCACAAAAGCCAT+TGG - chr3_3:66203361-66203380 None:intergenic 35.0%
GGTTGAAATGAATTATCCGT+TGG + chr3_3:66203457-66203476 Msa0465750:intron 35.0%
ATTGCTTCTTAGTGAATGTG+AGG + chr3_3:66203498-66203517 Msa0465750:intron 35.0%
TACAAATTGTGAGTCATGGA+TGG - chr3_3:66203653-66203672 None:intergenic 35.0%
! CTTCTTAAAATTTTGCCAGC+TGG - chr3_3:66203812-66203831 None:intergenic 35.0%
GTTGATATTGCTCTAACTAG+TGG + chr3_3:66203839-66203858 Msa0465750:CDS 35.0%
GCCAAAAGAATAGCTGATAA+AGG - chr3_3:66204035-66204054 None:intergenic 35.0%
AATGCAATGGTTCCCATAAA+TGG - chr3_3:66202514-66202533 None:intergenic 35.0%
GTTCATTGTAGATAGACTGA+AGG + chr3_3:66202970-66202989 Msa0465750:CDS 35.0%
TAAAGATAGGCGATAGCAAA+TGG - chr3_3:66203060-66203079 None:intergenic 35.0%
GGGGAACCTGAAAAATAATA+AGG - chr3_3:66203088-66203107 None:intergenic 35.0%
TTTCTTACCACAAAAGCCAT+TGG - chr3_3:66203361-66203380 None:intergenic 35.0%
GGTTGAAATGAATTATCCGT+TGG + chr3_3:66203457-66203476 Msa0465750:intron 35.0%
ATTGCTTCTTAGTGAATGTG+AGG + chr3_3:66203498-66203517 Msa0465750:intron 35.0%
TACAAATTGTGAGTCATGGA+TGG - chr3_3:66203653-66203672 None:intergenic 35.0%
! CTTCTTAAAATTTTGCCAGC+TGG - chr3_3:66203812-66203831 None:intergenic 35.0%
GTTGATATTGCTCTAACTAG+TGG + chr3_3:66203839-66203858 Msa0465750:CDS 35.0%
GCCAAAAGAATAGCTGATAA+AGG - chr3_3:66204035-66204054 None:intergenic 35.0%
AAGATAGTTGACCCTGTTAC+TGG + chr3_3:66202409-66202428 Msa0465750:CDS 40.0%
AGATAGTTGACCCTGTTACT+GGG + chr3_3:66202410-66202429 Msa0465750:CDS 40.0%
ATAATGACTCCCCAGTAACA+GGG - chr3_3:66202423-66202442 None:intergenic 40.0%
AATGGTTCCCATAAATGGAG+AGG - chr3_3:66202509-66202528 None:intergenic 40.0%
TATGTAATAGATGGGCGACA+AGG - chr3_3:66202746-66202765 None:intergenic 40.0%
AGGTTATGTAGGAGATGACA+AGG + chr3_3:66202871-66202890 Msa0465750:intron 40.0%
TGTTGAGACATTGGATTCAG+AGG + chr3_3:66202898-66202917 Msa0465750:CDS 40.0%
GTTGAGACATTGGATTCAGA+GGG + chr3_3:66202899-66202918 Msa0465750:CDS 40.0%
TATGTGCTCTAATTCAGCTG+GGG - chr3_3:66203108-66203127 None:intergenic 40.0%
GTATGTGCTCTAATTCAGCT+GGG - chr3_3:66203109-66203128 None:intergenic 40.0%
AGTATGTGCTCTAATTCAGC+TGG - chr3_3:66203110-66203129 None:intergenic 40.0%
AGCATCAGCAATTTCAGGAT+TGG - chr3_3:66203138-66203157 None:intergenic 40.0%
!! ATACTAATCTTTTGGGGACC+AGG + chr3_3:66203308-66203327 Msa0465750:intron 40.0%
! GCAAATTCCAATGGCTTTTG+TGG + chr3_3:66203351-66203370 Msa0465750:CDS 40.0%
! ATCGATGCACCGCTATATTT+TGG - chr3_3:66203581-66203600 None:intergenic 40.0%
GCTGTACAAATTGTGAGTCA+TGG - chr3_3:66203657-66203676 None:intergenic 40.0%
ACACGTCGTATCTTCTTGTA+CGG - chr3_3:66203761-66203780 None:intergenic 40.0%
TCTATTCCAAAATCTCCAGC+TGG + chr3_3:66203794-66203813 Msa0465750:CDS 40.0%
! ATTTTGCCAGCTGGAGATTT+TGG - chr3_3:66203803-66203822 None:intergenic 40.0%
AAGATAGTTGACCCTGTTAC+TGG + chr3_3:66202409-66202428 Msa0465750:CDS 40.0%
AGATAGTTGACCCTGTTACT+GGG + chr3_3:66202410-66202429 Msa0465750:CDS 40.0%
ATAATGACTCCCCAGTAACA+GGG - chr3_3:66202423-66202442 None:intergenic 40.0%
AATGGTTCCCATAAATGGAG+AGG - chr3_3:66202509-66202528 None:intergenic 40.0%
TATGTAATAGATGGGCGACA+AGG - chr3_3:66202746-66202765 None:intergenic 40.0%
AGGTTATGTAGGAGATGACA+AGG + chr3_3:66202871-66202890 Msa0465750:intron 40.0%
TGTTGAGACATTGGATTCAG+AGG + chr3_3:66202898-66202917 Msa0465750:CDS 40.0%
GTTGAGACATTGGATTCAGA+GGG + chr3_3:66202899-66202918 Msa0465750:CDS 40.0%
TATGTGCTCTAATTCAGCTG+GGG - chr3_3:66203108-66203127 None:intergenic 40.0%
GTATGTGCTCTAATTCAGCT+GGG - chr3_3:66203109-66203128 None:intergenic 40.0%
AGTATGTGCTCTAATTCAGC+TGG - chr3_3:66203110-66203129 None:intergenic 40.0%
AGCATCAGCAATTTCAGGAT+TGG - chr3_3:66203138-66203157 None:intergenic 40.0%
!! ATACTAATCTTTTGGGGACC+AGG + chr3_3:66203308-66203327 Msa0465750:intron 40.0%
! GCAAATTCCAATGGCTTTTG+TGG + chr3_3:66203351-66203370 Msa0465750:CDS 40.0%
! ATCGATGCACCGCTATATTT+TGG - chr3_3:66203581-66203600 None:intergenic 40.0%
GCTGTACAAATTGTGAGTCA+TGG - chr3_3:66203657-66203676 None:intergenic 40.0%
ACACGTCGTATCTTCTTGTA+CGG - chr3_3:66203761-66203780 None:intergenic 40.0%
TCTATTCCAAAATCTCCAGC+TGG + chr3_3:66203794-66203813 Msa0465750:CDS 40.0%
! ATTTTGCCAGCTGGAGATTT+TGG - chr3_3:66203803-66203822 None:intergenic 40.0%
GGATATGGTCTGACTGAAAG+TGG + chr3_3:66202304-66202323 Msa0465750:five_prime_UTR 45.0%
TATGGTCTGACTGAAAGTGG+AGG + chr3_3:66202307-66202326 Msa0465750:five_prime_UTR 45.0%
TTTGATGAAGCTAAGCGCCA+CGG + chr3_3:66202352-66202371 Msa0465750:CDS 45.0%
GGGTCGACTAGCAGAAAATA+TGG + chr3_3:66202381-66202400 Msa0465750:CDS 45.0%
GATAGTTGACCCTGTTACTG+GGG + chr3_3:66202411-66202430 Msa0465750:CDS 45.0%
GATAATGACTCCCCAGTAAC+AGG - chr3_3:66202424-66202443 None:intergenic 45.0%
TCATTATCTCCTGGCCAGAA+AGG + chr3_3:66202436-66202455 Msa0465750:CDS 45.0%
CATTATCTCCTGGCCAGAAA+GGG + chr3_3:66202437-66202456 Msa0465750:CDS 45.0%
ATTATCTCCTGGCCAGAAAG+GGG + chr3_3:66202438-66202457 Msa0465750:CDS 45.0%
CGAGGACCAACAATCATGAA+AGG + chr3_3:66202472-66202491 Msa0465750:CDS 45.0%
! TCTTATTATGGTGCCAGTGC+AGG + chr3_3:66202851-66202870 Msa0465750:intron 45.0%
ATCTCCTACATAACCTGCAC+TGG - chr3_3:66202867-66202886 None:intergenic 45.0%
AGACATTGGATTCAGAGGGA+TGG + chr3_3:66202903-66202922 Msa0465750:CDS 45.0%
TCAGGTGATGCATTGTGAAG+TGG + chr3_3:66203015-66203034 Msa0465750:intron 45.0%
ATGTGCTCTAATTCAGCTGG+GGG - chr3_3:66203107-66203126 None:intergenic 45.0%
ACTGCAGCATCAGCAATTTC+AGG - chr3_3:66203143-66203162 None:intergenic 45.0%
TGCATCTTCATCAGGATACC+TGG - chr3_3:66203329-66203348 None:intergenic 45.0%
AGGTATCCTGATGAAGATGC+AGG + chr3_3:66203328-66203347 Msa0465750:intron 45.0%
GGTATCCTGATGAAGATGCA+GGG + chr3_3:66203329-66203348 Msa0465750:intron 45.0%
ATTTGCCCTGCATCTTCATC+AGG - chr3_3:66203337-66203356 None:intergenic 45.0%
AGATGCAGGGCAAATTCCAA+TGG + chr3_3:66203342-66203361 Msa0465750:CDS 45.0%
! GCTTTTGTGGTAAGAAAGCC+AGG + chr3_3:66203364-66203383 Msa0465750:CDS 45.0%
GTGAGGATTGATTGTGAGCT+TGG + chr3_3:66203515-66203534 Msa0465750:intron 45.0%
GGATATGGTCTGACTGAAAG+TGG + chr3_3:66202304-66202323 Msa0465750:five_prime_UTR 45.0%
TATGGTCTGACTGAAAGTGG+AGG + chr3_3:66202307-66202326 Msa0465750:five_prime_UTR 45.0%
TTTGATGAAGCTAAGCGCCA+CGG + chr3_3:66202352-66202371 Msa0465750:CDS 45.0%
GGGTCGACTAGCAGAAAATA+TGG + chr3_3:66202381-66202400 Msa0465750:CDS 45.0%
GATAGTTGACCCTGTTACTG+GGG + chr3_3:66202411-66202430 Msa0465750:CDS 45.0%
GATAATGACTCCCCAGTAAC+AGG - chr3_3:66202424-66202443 None:intergenic 45.0%
TCATTATCTCCTGGCCAGAA+AGG + chr3_3:66202436-66202455 Msa0465750:CDS 45.0%
CATTATCTCCTGGCCAGAAA+GGG + chr3_3:66202437-66202456 Msa0465750:CDS 45.0%
ATTATCTCCTGGCCAGAAAG+GGG + chr3_3:66202438-66202457 Msa0465750:CDS 45.0%
CGAGGACCAACAATCATGAA+AGG + chr3_3:66202472-66202491 Msa0465750:CDS 45.0%
! TCTTATTATGGTGCCAGTGC+AGG + chr3_3:66202851-66202870 Msa0465750:intron 45.0%
ATCTCCTACATAACCTGCAC+TGG - chr3_3:66202867-66202886 None:intergenic 45.0%
AGACATTGGATTCAGAGGGA+TGG + chr3_3:66202903-66202922 Msa0465750:CDS 45.0%
TCAGGTGATGCATTGTGAAG+TGG + chr3_3:66203015-66203034 Msa0465750:intron 45.0%
ATGTGCTCTAATTCAGCTGG+GGG - chr3_3:66203107-66203126 None:intergenic 45.0%
ACTGCAGCATCAGCAATTTC+AGG - chr3_3:66203143-66203162 None:intergenic 45.0%
TGCATCTTCATCAGGATACC+TGG - chr3_3:66203329-66203348 None:intergenic 45.0%
AGGTATCCTGATGAAGATGC+AGG + chr3_3:66203328-66203347 Msa0465750:intron 45.0%
GGTATCCTGATGAAGATGCA+GGG + chr3_3:66203329-66203348 Msa0465750:intron 45.0%
ATTTGCCCTGCATCTTCATC+AGG - chr3_3:66203337-66203356 None:intergenic 45.0%
AGATGCAGGGCAAATTCCAA+TGG + chr3_3:66203342-66203361 Msa0465750:CDS 45.0%
! GCTTTTGTGGTAAGAAAGCC+AGG + chr3_3:66203364-66203383 Msa0465750:CDS 45.0%
GTGAGGATTGATTGTGAGCT+TGG + chr3_3:66203515-66203534 Msa0465750:intron 45.0%
GTCTGACTGAAAGTGGAGGA+GGG + chr3_3:66202311-66202330 Msa0465750:five_prime_UTR 50.0%
TCTGACTGAAAGTGGAGGAG+GGG + chr3_3:66202312-66202331 Msa0465750:five_prime_UTR 50.0%
ACTGGGGAGTCATTATCTCC+TGG + chr3_3:66202427-66202446 Msa0465750:CDS 50.0%
CAAGGCGACTGTTGAGACAT+TGG + chr3_3:66202889-66202908 Msa0465750:CDS 50.0%
AAGCAACATCACTGCAGCTC+AGG + chr3_3:66203387-66203406 Msa0465750:CDS 50.0%
! CATGGACTATGTTGCGAAGC+AGG + chr3_3:66203411-66203430 Msa0465750:CDS 50.0%
GTCTGACTGAAAGTGGAGGA+GGG + chr3_3:66202311-66202330 Msa0465750:five_prime_UTR 50.0%
TCTGACTGAAAGTGGAGGAG+GGG + chr3_3:66202312-66202331 Msa0465750:five_prime_UTR 50.0%
ACTGGGGAGTCATTATCTCC+TGG + chr3_3:66202427-66202446 Msa0465750:CDS 50.0%
CAAGGCGACTGTTGAGACAT+TGG + chr3_3:66202889-66202908 Msa0465750:CDS 50.0%
AAGCAACATCACTGCAGCTC+AGG + chr3_3:66203387-66203406 Msa0465750:CDS 50.0%
! CATGGACTATGTTGCGAAGC+AGG + chr3_3:66203411-66203430 Msa0465750:CDS 50.0%
GGTCTGACTGAAAGTGGAGG+AGG + chr3_3:66202310-66202329 Msa0465750:five_prime_UTR 55.0%
GGAGGGGCAGCAAGAATGAT+AGG + chr3_3:66202328-66202347 Msa0465750:exon 55.0%
GCAACCAAAGCTCCCCTTTC+TGG - chr3_3:66202453-66202472 None:intergenic 55.0%
! AAAGGGGAGCTTTGGTTGCG+AGG + chr3_3:66202454-66202473 Msa0465750:CDS 55.0%
GGTGCCAGTGCAGGTTATGT+AGG + chr3_3:66202860-66202879 Msa0465750:intron 55.0%
TCAGAGGGATGGCTGAAGAC+CGG + chr3_3:66202914-66202933 Msa0465750:CDS 55.0%
! GCTGCAGTGATGTTGCTTCC+TGG - chr3_3:66203385-66203404 None:intergenic 55.0%
CATCACTGCAGCTCAGGTCA+TGG + chr3_3:66203393-66203412 Msa0465750:CDS 55.0%
GGTCTGACTGAAAGTGGAGG+AGG + chr3_3:66202310-66202329 Msa0465750:five_prime_UTR 55.0%
GGAGGGGCAGCAAGAATGAT+AGG + chr3_3:66202328-66202347 Msa0465750:exon 55.0%
GCAACCAAAGCTCCCCTTTC+TGG - chr3_3:66202453-66202472 None:intergenic 55.0%
! AAAGGGGAGCTTTGGTTGCG+AGG + chr3_3:66202454-66202473 Msa0465750:CDS 55.0%
GGTGCCAGTGCAGGTTATGT+AGG + chr3_3:66202860-66202879 Msa0465750:intron 55.0%
TCAGAGGGATGGCTGAAGAC+CGG + chr3_3:66202914-66202933 Msa0465750:CDS 55.0%
! GCTGCAGTGATGTTGCTTCC+TGG - chr3_3:66203385-66203404 None:intergenic 55.0%
CATCACTGCAGCTCAGGTCA+TGG + chr3_3:66203393-66203412 Msa0465750:CDS 55.0%
AGCTAAGCGCCACGGTTCTG+TGG + chr3_3:66202360-66202379 Msa0465750:CDS 60.0%
GCTAAGCGCCACGGTTCTGT+GGG + chr3_3:66202361-66202380 Msa0465750:CDS 60.0%
CTAGTCGACCCACAGAACCG+TGG - chr3_3:66202372-66202391 None:intergenic 60.0%
! CAAAGCTCCCCTTTCTGGCC+AGG - chr3_3:66202448-66202467 None:intergenic 60.0%
CTGGCCAGAAAGGGGAGCTT+TGG + chr3_3:66202446-66202465 Msa0465750:CDS 60.0%
AGCTAAGCGCCACGGTTCTG+TGG + chr3_3:66202360-66202379 Msa0465750:CDS 60.0%
GCTAAGCGCCACGGTTCTGT+GGG + chr3_3:66202361-66202380 Msa0465750:CDS 60.0%
CTAGTCGACCCACAGAACCG+TGG - chr3_3:66202372-66202391 None:intergenic 60.0%
! CAAAGCTCCCCTTTCTGGCC+AGG - chr3_3:66202448-66202467 None:intergenic 60.0%
CTGGCCAGAAAGGGGAGCTT+TGG + chr3_3:66202446-66202465 Msa0465750:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 66202302 66204126 66202302 ID=Msa0465750;Name=Msa0465750
chr3_3 mRNA 66202302 66204126 66202302 ID=Msa0465750-mRNA-1;Parent=Msa0465750;Name=Msa0465750-mRNA-1;_AED=0.03;_eAED=0.03;_QI=41|1|1|1|1|1|5|249|197
chr3_3 exon 66202302 66202493 66202302 ID=Msa0465750-mRNA-1:exon:14939;Parent=Msa0465750-mRNA-1
chr3_3 exon 66202873 66203018 66202873 ID=Msa0465750-mRNA-1:exon:14940;Parent=Msa0465750-mRNA-1
chr3_3 exon 66203101 66203168 66203101 ID=Msa0465750-mRNA-1:exon:14941;Parent=Msa0465750-mRNA-1
chr3_3 exon 66203330 66203432 66203330 ID=Msa0465750-mRNA-1:exon:14942;Parent=Msa0465750-mRNA-1
chr3_3 exon 66203752 66204126 66203752 ID=Msa0465750-mRNA-1:exon:14943;Parent=Msa0465750-mRNA-1
chr3_3 five_prime_UTR 66202302 66202342 66202302 ID=Msa0465750-mRNA-1:five_prime_utr;Parent=Msa0465750-mRNA-1
chr3_3 CDS 66202343 66202493 66202343 ID=Msa0465750-mRNA-1:cds;Parent=Msa0465750-mRNA-1
chr3_3 CDS 66202873 66203018 66202873 ID=Msa0465750-mRNA-1:cds;Parent=Msa0465750-mRNA-1
chr3_3 CDS 66203101 66203168 66203101 ID=Msa0465750-mRNA-1:cds;Parent=Msa0465750-mRNA-1
chr3_3 CDS 66203330 66203432 66203330 ID=Msa0465750-mRNA-1:cds;Parent=Msa0465750-mRNA-1
chr3_3 CDS 66203752 66203877 66203752 ID=Msa0465750-mRNA-1:cds;Parent=Msa0465750-mRNA-1
chr3_3 three_prime_UTR 66203878 66204126 66203878 ID=Msa0465750-mRNA-1:three_prime_utr;Parent=Msa0465750-mRNA-1
Gene Sequence

>Msa0465750

ATGATAGGTTTTGATGAAGCTAAGCGCCACGGTTCTGTGGGTCGACTAGCAGAAAATATGGAAGCTAAGATAGTTGACCCTGTTACTGGGGAGTCATTATCTCCTGGCCAGAAAGGGGAGCTTTGGTTGCGAGGACCAACAATCATGAAAGGTTATGTAGGAGATGACAAGGCGACTGTTGAGACATTGGATTCAGAGGGATGGCTGAAGACCGGTGATCTTTGTTATTTTGACTCAGATGGTTATCTGTTCATTGTAGATAGACTGAAGGAATTGATCAAATACAAAGCTTATCAGGTTCCCCCAGCTGAATTAGAGCACATACTTCATACCAATCCTGAAATTGCTGATGCTGCAGTAGTCCCGTATCCTGATGAAGATGCAGGGCAAATTCCAATGGCTTTTGTGGTAAGAAAGCCAGGAAGCAACATCACTGCAGCTCAGGTCATGGACTATGTTGCGAAGCAGGTGACACCGTACAAGAAGATACGACGTGTTTCTTTTATCAACTCTATTCCAAAATCTCCAGCTGGCAAAATTTTAAGAAGAGAATTAGTTGATATTGCTCTAACTAGTGGTTCATCTAAATTGTGA
Protein sequence

>Msa0465750

MIGFDEAKRHGSVGRLAENMEAKIVDPVTGESLSPGQKGELWLRGPTIMKGYVGDDKATVETLDSEGWLKTGDLCYFDSDGYLFIVDRLKELIKYKAYQVPPAELEHILHTNPEIADAAVVPYPDEDAGQIPMAFVVRKPGSNITAAQVMDYVAKQVTPYKKIRRVSFINSIPKSPAGKILRRELVDIALTSGSSKL*