AlfalfaGEDB | Msa0466430

M. sativa cultivar zhongmu-4 / Msa0466430

KEGG: K09338
GO: -
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0466430	XP_003601381.1	97.255	255	7	0	1	255	1	255	1.99e-180	505

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0466430	sp\|P46667\|ATHB5_ARATH	53.571	168	65	4	1	155	1	168	3.20e-48	164

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0466430	G7J6Q0	97.255	255	7	0	1	255	1	255	0.0	505

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TFs/TRs:

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Gene ID	Type	Classification
Msa0466430	TF	HB-HD-ZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0466430	Msa1145160	0.890022	1.433502e-73	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0466430	MtrunA17_Chr3g0119291	97.255	255	7	0	1	255	1	255	0.0	505
Msa0466430	MtrunA17_Chr8g0381741	38.312	308	131	9	1	255	1	302	6.99e-55	179
Msa0466430	MtrunA17_Chr3g0123691	40.940	298	128	11	1	255	1	293	5.06e-46	155
Msa0466430	MtrunA17_Chr6g0453021	45.960	198	92	4	42	238	82	265	3.86e-40	139
Msa0466430	MtrunA17_Chr8g0362151	57.724	123	51	1	46	168	64	185	6.24e-38	134
Msa0466430	MtrunA17_Chr5g0416391	57.600	125	52	1	44	168	62	185	1.27e-37	133
Msa0466430	MtrunA17_Chr5g0415941	56.667	120	52	0	47	166	75	194	1.29e-37	134
Msa0466430	MtrunA17_Chr4g0060031	63.810	105	38	0	58	162	94	198	9.47e-36	130
Msa0466430	MtrunA17_Chr8g0362751	61.538	104	40	0	59	162	89	192	1.55e-35	128
Msa0466430	MtrunA17_Chr6g0450991	61.682	107	41	0	58	164	66	172	1.83e-34	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0466430	AT5G65310.1	53.571	168	65	4	1	155	1	168	3.25e-49	164
Msa0466430	AT5G65310.2	59.441	143	47	2	24	155	8	150	2.02e-48	162
Msa0466430	AT4G40060.1	34.740	308	124	9	1	255	1	284	4.73e-44	150
Msa0466430	AT2G22430.1	49.057	159	75	2	1	155	2	158	1.84e-42	147
Msa0466430	AT1G69780.1	60.163	123	48	1	46	168	72	193	1.68e-39	139
Msa0466430	AT5G15150.1	58.594	128	49	3	42	166	95	221	1.03e-37	134
Msa0466430	AT1G26960.1	43.500	200	98	5	41	235	53	242	2.25e-36	129
Msa0466430	AT3G01220.1	43.548	186	95	4	44	224	70	250	2.56e-35	127
Msa0466430	AT3G01470.1	60.000	100	40	0	56	155	65	164	7.53e-33	121
Msa0466430	AT3G61890.1	39.161	143	82	2	62	202	33	172	3.83e-27	105

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Find 57 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACCATTGAAACATAT+AGG	0.475600	3_3:+67054141	None:intergenic
AAATTAGTAGTGTTCTACAA+GGG	0.702473	3_3:-67054964	Msa0466430:five_prime_UTR
AACCAAATTGAAACTTGTCT+AGG	0.545141	3_3:+67054545	None:intergenic
AACCTAGACAAGTTTCAATT+TGG	0.262579	3_3:-67054547	Msa0466430:CDS
AACCTTAAGAACATCAAAAT+TGG	0.244715	3_3:+67054460	None:intergenic
AATAGTAGTAATTGTGTGTG+TGG	0.626966	3_3:-67055080	Msa0466430:five_prime_UTR
AATGAAGAGGTTCAATAACT+CGG	0.609369	3_3:-67054921	Msa0466430:CDS
ACCAAATTCATCATTGAACT+TGG	0.453246	3_3:+67054117	None:intergenic
ACGAACGAAAACCAAACAAT+TGG	0.484787	3_3:-67054507	Msa0466430:CDS
AGAGGATAGGACAGGACATA+TGG	0.489411	3_3:+67055212	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAGTAGTAATTGTGTGTG+TGG	-	chr3_3:67054106-67054125	Msa0466430:CDS	30.0%
CATCTATATTATCCCAACTT+TGG	-	chr3_3:67054188-67054207	Msa0466430:CDS	30.0%
AATGAAGAGGTTCAATAACT+CGG	-	chr3_3:67054265-67054284	Msa0466430:CDS	30.0%
AACCTAGACAAGTTTCAATT+TGG	-	chr3_3:67054639-67054658	Msa0466430:CDS	30.0%
AACCAAATTGAAACTTGTCT+AGG	+	chr3_3:67054644-67054663	None:intergenic	30.0%
CTTTCTATACCTCTTGAAAT+TGG	+	chr3_3:67054967-67054986	None:intergenic	30.0%
GATTCTCCTATATGTTTCAA+TGG	-	chr3_3:67055039-67055058	Msa0466430:five_prime_UTR	30.0%
ACCAAATTCATCATTGAACT+TGG	+	chr3_3:67055072-67055091	None:intergenic	30.0%
GAATCAATTTATGAGAATGG+AGG	-	chr3_3:67055134-67055153	Msa0466430:five_prime_UTR	30.0%
TCACTTGGAATTACAAAGTA+GGG	+	chr3_3:67055215-67055234	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	67053874	67055334	67053874	ID=Msa0466430;Name=Msa0466430
chr3_3	mRNA	67053874	67055334	67053874	ID=Msa0466430-mRNA-1;Parent=Msa0466430;Name=Msa0466430-mRNA-1;_AED=0.04;_eAED=0.04;_QI=392\|1\|1\|1\|1\|1\|3\|110\|255
chr3_3	exon	67053874	67054310	67053874	ID=Msa0466430-mRNA-1:exon:15406;Parent=Msa0466430-mRNA-1
chr3_3	exon	67054415	67054794	67054415	ID=Msa0466430-mRNA-1:exon:15405;Parent=Msa0466430-mRNA-1
chr3_3	exon	67054882	67055334	67054882	ID=Msa0466430-mRNA-1:exon:15404;Parent=Msa0466430-mRNA-1
chr3_3	five_prime_UTR	67054943	67055334	67054943	ID=Msa0466430-mRNA-1:five_prime_utr;Parent=Msa0466430-mRNA-1
chr3_3	CDS	67054882	67054942	67054882	ID=Msa0466430-mRNA-1:cds;Parent=Msa0466430-mRNA-1
chr3_3	CDS	67054415	67054794	67054415	ID=Msa0466430-mRNA-1:cds;Parent=Msa0466430-mRNA-1
chr3_3	CDS	67053984	67054310	67053984	ID=Msa0466430-mRNA-1:cds;Parent=Msa0466430-mRNA-1
chr3_3	three_prime_UTR	67053874	67053983	67053874	ID=Msa0466430-mRNA-1:three_prime_utr;Parent=Msa0466430-mRNA-1

Gene Sequence

>Msa0466430

ATGAAGAGGTTCAATAACTCGGAAACTATGGCTTCTTTACTTTCCATGTACCAATCCAAAGAGGTGGATGTGGATCAGAGGAATGAGACAAAAGGTTACAGTGAAGAATTTCAAGCAATGTTAGATAGATTAGAGCAAGAAGATAGCTATGAAGATGGTAGTTCAATGTTGGAGAAGAAAAGAAGACTTGGTTATGATCAAGTAAAAGCTTTAGAAAAGAGCTTTGAATTGGACAACAAGCTAGAGCCAGAGAGAAAAGTGAAGTTAGCTGAAGATTTAGGCTTGCAACCTAGACAAGTTTCAATTTGGTTCCAAAATCGTCGAGCACGAACGAAAACCAAACAATTGGAAAGAGATTATAGTGTTCTTAAGTCCAATTTTGATGTTCTTAAGGTTGAGTATAGTAATCTTCAACAAGAGAATGAGACTCTTACTAGAAAGTTAAGAGAGTTGAAAGCGAAATTGTTAAGAGTTGAAAGCGATGAGTTAGAGAAAGAAGTAGAATGTCCAATTTCAAGAGGTATAGAAAGAGGAGGTTCAGAAAGTAATTCAAGTGGTGTAATTAAGGAAGAAAGTAATGTTGATTCTCCTATATGTTTCAATGGTTCTTTGCCAAGTTCAATGATGAATTTGGTTCATTATTATGATTCAAGAGGTTCTCAAGAAAATGTGTTTCAGAATCAATTTATGAGAATGGAGGAGCAGAATTTCTTAAGTAATAGTGAATTATTCAGTTTCTTTTCAGTTGATCAATCCCCTACTTTGTAA

Protein sequence

>Msa0466430

MKRFNNSETMASLLSMYQSKEVDVDQRNETKGYSEEFQAMLDRLEQEDSYEDGSSMLEKKRRLGYDQVKALEKSFELDNKLEPERKVKLAEDLGLQPRQVSIWFQNRRARTKTKQLERDYSVLKSNFDVLKVEYSNLQQENETLTRKLRELKAKLLRVESDELEKEVECPISRGIERGGSESNSSGVIKEESNVDSPICFNGSLPSSMMNLVHYYDSRGSQENVFQNQFMRMEEQNFLSNSELFSFFSVDQSPTL*