AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0466860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0466860 MtrunA17_Chr3g0121041 83.168 202 9 2 3 179 2 203 1.30e-117 331
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0466860 AT2G04845.1 57.286 199 60 2 6 179 3 201 3.76e-74 222
Msa0466860 AT2G04845.5 58.621 174 47 2 6 154 3 176 1.72e-69 210
Msa0466860 AT2G04845.4 55.376 186 71 3 6 179 3 188 1.36e-68 207
Msa0466860 AT2G04845.3 55.376 186 71 3 6 179 3 188 1.84e-68 207
Msa0466860 AT2G04845.2 58.788 165 57 2 7 160 4 168 4.94e-68 206

Find 61 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAGCATCTCCTCATTCT+TGG 0.266496 3_3:+67980823 None:intergenic
TTGGATAAAGACTTGCTTCT+TGG 0.270404 3_3:-67984563 Msa0466860:CDS
CCATCTTAACAAGGATAAAA+TGG 0.271503 3_3:+67980579 None:intergenic
TGCATCCATTCATGATATTT+CGG 0.285663 3_3:+67984695 None:intergenic
AAAGGAAATGTGAGTTTAGA+AGG 0.318086 3_3:-67984758 Msa0466860:CDS
CAAGGAAACTTTCATTCTCT+TGG 0.318845 3_3:-67984582 Msa0466860:CDS
GAGTTCGATGGCGGCGGCGG+CGG 0.327667 3_3:-67984789 Msa0466860:exon
GCCTTTGCTATTGAGAAGTT+GGG 0.345000 3_3:-67983425 Msa0466860:CDS
CATAGCCGTGGCAAAGGACT+TGG 0.395075 3_3:-67983473 Msa0466860:CDS
GCGGAGTTCGATGGCGGCGG+CGG 0.403087 3_3:-67984792 Msa0466860:exon
TGAGGAGATGCTTGGTTTGA+TGG 0.406506 3_3:-67980816 Msa0466860:CDS
CTTCTTGGAACCTTCTCTCA+TGG 0.417969 3_3:-67984548 Msa0466860:CDS
GGAACGTGAGGTTCCATGTA+AGG 0.421077 3_3:+67984716 None:intergenic
GCGGCGGCGGCGAGGAAGAA+AGG 0.437378 3_3:-67984776 Msa0466860:CDS
GGCCTTTGCTATTGAGAAGT+TGG 0.437922 3_3:-67983426 Msa0466860:CDS
ATACTCTTGTTCGAGAGAAA+GGG 0.455235 3_3:+67984637 None:intergenic
TTTCTGCAGCTTATGCGTTA+CGG 0.455651 3_3:+67984858 None:intergenic
GATACTCTTGTTCGAGAGAA+AGG 0.456194 3_3:+67984636 None:intergenic
CTTGTTGAGATCACGTGACC+AGG 0.456827 3_3:+67984601 None:intergenic
ATTTCAACCTCTGCTATTCG+AGG 0.459197 3_3:+67984377 None:intergenic
GCTGCGCTTTGCTCTTGCCA+CGG 0.465838 3_3:+67984816 None:intergenic
GCGGCGGAGTTCGATGGCGG+CGG 0.470509 3_3:-67984795 Msa0466860:exon
ATTTGTTTGACAGGTGACTT+TGG 0.470985 3_3:-67980864 Msa0466860:intron
GTCAAATTCTTACACTTGAA+AGG 0.486297 3_3:+67980637 None:intergenic
ACGTTCCGAAATATCATGAA+TGG 0.490153 3_3:-67984700 Msa0466860:CDS
CATGGTCAACCCCACGTTGA+AGG 0.491566 3_3:-67984530 Msa0466860:intron
GGTAATACTGGTCCCTTACA+TGG 0.495614 3_3:-67984729 Msa0466860:CDS
AGCGCAGCGGCGGAGTTCGA+TGG 0.496615 3_3:-67984801 Msa0466860:exon
ACATTATTGACCTTCAACGT+GGG 0.501922 3_3:+67984520 None:intergenic
CTAATGTCACAATCTTGTAT+TGG 0.503254 3_3:+67980677 None:intergenic
TTGTTCGAGAGAAAGGGGTT+CGG 0.511970 3_3:+67984643 None:intergenic
TGTGAGTTTAGAAGGAAAGA+AGG 0.512492 3_3:-67984750 Msa0466860:CDS
AGAAGGAAAGAAGGTAATAC+TGG 0.516666 3_3:-67984741 Msa0466860:CDS
GCCAAGAATGAGGAGATGCT+TGG 0.528942 3_3:-67980824 Msa0466860:CDS
GCAGTGGCTTGAAGAAGAGA+AGG 0.540091 3_3:+67984668 None:intergenic
TCACAATCTTGTATTGGTAA+TGG 0.544490 3_3:+67980683 None:intergenic
GCAAGAGCAAAGCGCAGCGG+CGG 0.545002 3_3:-67984811 Msa0466860:five_prime_UTR
AGTATCAGATGCAACTCTCC+TGG 0.549318 3_3:-67984619 Msa0466860:CDS
TGTTAGCATAGCCGTGGCAA+AGG 0.550626 3_3:-67983479 Msa0466860:intron
CCGCTATTTGCAGCTATGGT+TGG 0.553290 3_3:-67984434 Msa0466860:intron
GCAGCGGCGGAGTTCGATGG+CGG 0.564183 3_3:-67984798 Msa0466860:exon
TCATGATATTTCGGAACGTG+AGG 0.572117 3_3:+67984704 None:intergenic
GAGGAGATGCTTGGTTTGAT+GGG 0.572308 3_3:-67980815 Msa0466860:CDS
CCTTTGCTATTGAGAAGTTG+GGG 0.572445 3_3:-67983424 Msa0466860:CDS
AATAACACAAGCCAAGAATG+AGG 0.587170 3_3:-67980834 Msa0466860:CDS
AGAAAGGGGTTCGGAAGCAG+TGG 0.587837 3_3:+67984652 None:intergenic
CCTTTCCAAGTCCTTTGCCA+CGG 0.588992 3_3:+67983468 None:intergenic
AACATTATTGACCTTCAACG+TGG 0.591929 3_3:+67984519 None:intergenic
TACTCTTGTTCGAGAGAAAG+GGG 0.600981 3_3:+67984638 None:intergenic
CCGTGGCAAAGGACTTGGAA+AGG 0.603951 3_3:-67983468 Msa0466860:CDS
CGTGTGAACCCATCTTAACA+AGG 0.606372 3_3:+67980570 None:intergenic
CCCCAACTTCTCAATAGCAA+AGG 0.607006 3_3:+67983424 None:intergenic
CTGGTCACGTGATCTCAACA+AGG 0.618601 3_3:-67984600 Msa0466860:CDS
GAACGTGAGGTTCCATGTAA+GGG 0.624781 3_3:+67984717 None:intergenic
AAAATCAATTGGTCAGTCCG+TGG 0.631608 3_3:-67984833 Msa0466860:five_prime_UTR
CATTATTGACCTTCAACGTG+GGG 0.634827 3_3:+67984521 None:intergenic
CAATGATCCTCGAATAGCAG+AGG 0.644727 3_3:-67984384 Msa0466860:intron
CAGTGGCTTGAAGAAGAGAA+GGG 0.656481 3_3:+67984669 None:intergenic
GTGGGGTTGACCATGAGAGA+AGG 0.659157 3_3:+67984538 None:intergenic
AAATACGCACATCATGAACA+AGG 0.665884 3_3:+67980718 None:intergenic
GTGGCAAGAGCAAAGCGCAG+CGG 0.684523 3_3:-67984814 Msa0466860:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTAAATTATCTATTTA+GGG + chr3_3:67982174-67982193 None:intergenic 10.0%
!! ATATTCTAAATTATCTATTT+AGG + chr3_3:67982175-67982194 None:intergenic 10.0%
!! TTTGAATAAAAATGACATAA+TGG + chr3_3:67981189-67981208 None:intergenic 15.0%
!!! TTGAATATTTAAAATTGTGA+CGG + chr3_3:67981679-67981698 None:intergenic 15.0%
!!! TTTTAAATTTAGAACATAGA+TGG + chr3_3:67981798-67981817 None:intergenic 15.0%
!!! TGTTATTGTAATTTTAACTA+AGG - chr3_3:67984019-67984038 Msa0466860:intron 15.0%
!!! CTTTTTAAAATCAAACTTTT+CGG - chr3_3:67984165-67984184 Msa0466860:intron 15.0%
!! AAATGCTCAAAATTTGTATT+TGG - chr3_3:67981580-67981599 Msa0466860:CDS 20.0%
!!! AAAACTTTTCAGGTTAAAAT+TGG - chr3_3:67982037-67982056 Msa0466860:intron 20.0%
!! ATATTCTCGATTATCTATTT+AGG + chr3_3:67982337-67982356 None:intergenic 20.0%
!!! TTTCTATTTTTCAGATAAAC+AGG + chr3_3:67982555-67982574 None:intergenic 20.0%
!! TCTGAAAAATAGAAATTGAA+TGG - chr3_3:67982560-67982579 Msa0466860:intron 20.0%
!! CTGAAAAATAGAAATTGAAT+GGG - chr3_3:67982561-67982580 Msa0466860:intron 20.0%
!! TGAAAAATAGAAATTGAATG+GGG - chr3_3:67982562-67982581 Msa0466860:intron 20.0%
!! TGTTTCATAAACTACAAAAA+GGG + chr3_3:67983084-67983103 None:intergenic 20.0%
!! ATGTTTCATAAACTACAAAA+AGG + chr3_3:67983085-67983104 None:intergenic 20.0%
!!! ACACTATTTAAGATATATGA+TGG - chr3_3:67983227-67983246 Msa0466860:intron 20.0%
!!! TTTAAGATATATGATGGAAT+AGG - chr3_3:67983233-67983252 Msa0466860:intron 20.0%
!!! TGAAGTTACAGTTTTTTTAT+TGG - chr3_3:67983628-67983647 Msa0466860:intron 20.0%
!!! AAAATATTCTTTTAGAACCA+TGG - chr3_3:67983708-67983727 Msa0466860:intron 20.0%
!!! TAATTTCAAATTTTCAACAG+AGG - chr3_3:67983902-67983921 Msa0466860:intron 20.0%
!!! CGAAAAGTTTGATTTTAAAA+AGG + chr3_3:67984167-67984186 None:intergenic 20.0%
!! ATGGTTAAAACAAAAACTTA+AGG + chr3_3:67984395-67984414 None:intergenic 20.0%
! CTGCAGAAAAAAAAATCAAT+TGG - chr3_3:67980591-67980610 Msa0466860:three_prime_UTR 25.0%
! ATATCTCAGATAAAAGTTAG+AGG - chr3_3:67981413-67981432 Msa0466860:intron 25.0%
!!! AAGTTAGAGGTGTTAAAAAT+AGG - chr3_3:67981426-67981445 Msa0466860:intron 25.0%
! GATGAAAATTACTTTAGTAC+AGG - chr3_3:67981457-67981476 Msa0466860:intron 25.0%
! ATGCTCTACTTGATTTATAA+AGG + chr3_3:67981483-67981502 None:intergenic 25.0%
!!! TGTCATCTCTAAAACATAAA+AGG - chr3_3:67981557-67981576 Msa0466860:intron 25.0%
! AATTTGTATTTGGTCTCAAT+TGG - chr3_3:67981590-67981609 Msa0466860:intron 25.0%
!!! GCATATGTATACTTTTTAAC+CGG - chr3_3:67981638-67981657 Msa0466860:intron 25.0%
!!! ATCTAGAAGTCTTATGTTTT+GGG - chr3_3:67981867-67981886 Msa0466860:intron 25.0%
! ATTGTCTTATGTATTAAGAC+AGG + chr3_3:67982264-67982283 None:intergenic 25.0%
! TGTCTTAATACATAAGACAA+TGG - chr3_3:67982263-67982282 Msa0466860:intron 25.0%
! GAATATAGAATCGAAATACA+AGG - chr3_3:67982391-67982410 Msa0466860:intron 25.0%
! TTTGTCTTATGTATTAAGAC+AGG + chr3_3:67982428-67982447 None:intergenic 25.0%
! TGTCTTAATACATAAGACAA+AGG - chr3_3:67982263-67982282 Msa0466860:intron 25.0%
! GTTAAGGTTATTATAGAACT+TGG + chr3_3:67982745-67982764 None:intergenic 25.0%
! ACCTTAACACACATTATAAA+TGG - chr3_3:67982757-67982776 Msa0466860:intron 25.0%
! ACCATTTATAATGTGTGTTA+AGG + chr3_3:67982761-67982780 None:intergenic 25.0%
! TTACTTCTTAATTTAATCGC+TGG - chr3_3:67982816-67982835 Msa0466860:intron 25.0%
!! TGAAAATTTTGTCCATCAAA+TGG + chr3_3:67982897-67982916 None:intergenic 25.0%
! ATTGATCAATTCAATGACTT+TGG + chr3_3:67983013-67983032 None:intergenic 25.0%
! TATAAATAGGAGAACACAAA+GGG + chr3_3:67983340-67983359 None:intergenic 25.0%
!!! ACAGTGCTATTTGTATAAAT+AGG + chr3_3:67983353-67983372 None:intergenic 25.0%
!! GAACTTAGGATTTAGAATAA+TGG + chr3_3:67983431-67983450 None:intergenic 25.0%
!! TCTACTTGTTAAATTCTCTA+TGG - chr3_3:67983482-67983501 Msa0466860:intron 25.0%
!!! AAAGCCTTTAAAATGAAAGA+TGG + chr3_3:67983583-67983602 None:intergenic 25.0%
!!! ATCTTTCATTTTAAAGGCTT+TGG - chr3_3:67983582-67983601 Msa0466860:intron 25.0%
! AATCAATTCTATAAAAGCCA+TGG + chr3_3:67983728-67983747 None:intergenic 25.0%
! GATTAAGGTAAATTGAAATC+TGG - chr3_3:67984129-67984148 Msa0466860:intron 25.0%
!! GTTTATTATGTTCTGAATGA+TGG + chr3_3:67984414-67984433 None:intergenic 25.0%
!! CATTTTATCCTTGTTAAGAT+GGG - chr3_3:67984857-67984876 Msa0466860:five_prime_UTR 25.0%
AAAGGAAATGTGAGTTTAGA+AGG - chr3_3:67980677-67980696 Msa0466860:three_prime_UTR 30.0%
TGCATCCATTCATGATATTT+CGG + chr3_3:67980743-67980762 None:intergenic 30.0%
! TCAGCAACTAGTTTATAAGT+AGG + chr3_3:67981108-67981127 None:intergenic 30.0%
GTCATGATTGTAACTTATTG+AGG - chr3_3:67981270-67981289 Msa0466860:intron 30.0%
!! AAGTTCTGGAAAACTTGTAT+TGG - chr3_3:67981753-67981772 Msa0466860:intron 30.0%
!! CATCTAGAAGTCTTATGTTT+TGG - chr3_3:67981866-67981885 Msa0466860:intron 30.0%
!!! CAGTTTTTTAAACAGGTTGA+TGG + chr3_3:67982080-67982099 None:intergenic 30.0%
CATCAACCTGTTTAAAAAAC+TGG - chr3_3:67982078-67982097 Msa0466860:intron 30.0%
AAAAAAAGATAGAGAAGCAG+CGG + chr3_3:67982109-67982128 None:intergenic 30.0%
CTCTTCATATTAAATGCATG+AGG - chr3_3:67982130-67982149 Msa0466860:intron 30.0%
GAATATAGAGTCGAAATACA+AGG - chr3_3:67982229-67982248 Msa0466860:intron 30.0%
TAGAATATCACTTCTGATAC+TGG - chr3_3:67982361-67982380 Msa0466860:intron 30.0%
! AAGACAAAGGTACATTATAC+AGG - chr3_3:67982440-67982459 Msa0466860:intron 30.0%
! AGACAAAGGTACATTATACA+GGG - chr3_3:67982441-67982460 Msa0466860:intron 30.0%
TTTCAGATAAACAGGTAGAA+AGG + chr3_3:67982547-67982566 None:intergenic 30.0%
AGCGGTTATGATTATGATTA+TGG + chr3_3:67982622-67982641 None:intergenic 30.0%
TTGTAAGTTGTTGTCAAACA+AGG - chr3_3:67982867-67982886 Msa0466860:intron 30.0%
AAACAAGGTTATCCATTTGA+TGG - chr3_3:67982882-67982901 Msa0466860:intron 30.0%
GAAGAATATTCTTTGAACCA+TGG - chr3_3:67983113-67983132 Msa0466860:intron 30.0%
AATCAATTCATATGAAGCCA+TGG + chr3_3:67983133-67983152 None:intergenic 30.0%
! AGAGTATTCTCTATTGTGAA+GGG + chr3_3:67983284-67983303 None:intergenic 30.0%
! TAGAGTATTCTCTATTGTGA+AGG + chr3_3:67983285-67983304 None:intergenic 30.0%
GTATAAATAGGAGAACACAA+AGG + chr3_3:67983341-67983360 None:intergenic 30.0%
TTAAAATGAAAGATGGACAG+AGG + chr3_3:67983576-67983595 None:intergenic 30.0%
! CTGTCCATCTTTCATTTTAA+AGG - chr3_3:67983576-67983595 Msa0466860:intron 30.0%
! ACATAGCAACATTTTCAAAG+AGG - chr3_3:67983869-67983888 Msa0466860:intron 30.0%
TAGTCAAAACAATTGAGACT+GGG + chr3_3:67984068-67984087 None:intergenic 30.0%
ATAGTCAAAACAATTGAGAC+TGG + chr3_3:67984069-67984088 None:intergenic 30.0%
AGTTACAAGCTAAGTGATTA+AGG - chr3_3:67984114-67984133 Msa0466860:intron 30.0%
TAACAGTTTGTGAAATCAAC+AGG + chr3_3:67984236-67984255 None:intergenic 30.0%
AAGAAGAAAACCGCATATAA+CGG + chr3_3:67984512-67984531 None:intergenic 30.0%
!! TTGTTCCTAATTTGTTTGAC+AGG - chr3_3:67984562-67984581 Msa0466860:CDS 30.0%
! TCACAATCTTGTATTGGTAA+TGG + chr3_3:67984755-67984774 None:intergenic 30.0%
CTAATGTCACAATCTTGTAT+TGG + chr3_3:67984761-67984780 None:intergenic 30.0%
GTCAAATTCTTACACTTGAA+AGG + chr3_3:67984801-67984820 None:intergenic 30.0%
CCATCTTAACAAGGATAAAA+TGG + chr3_3:67984859-67984878 None:intergenic 30.0%
! CCATTTTATCCTTGTTAAGA+TGG - chr3_3:67984856-67984875 Msa0466860:five_prime_UTR 30.0%
TGTGAGTTTAGAAGGAAAGA+AGG - chr3_3:67980685-67980704 Msa0466860:three_prime_UTR 35.0%
AGAAGGAAAGAAGGTAATAC+TGG - chr3_3:67980694-67980713 Msa0466860:three_prime_UTR 35.0%
ACGTTCCGAAATATCATGAA+TGG - chr3_3:67980735-67980754 Msa0466860:three_prime_UTR 35.0%
ATACTCTTGTTCGAGAGAAA+GGG + chr3_3:67980801-67980820 None:intergenic 35.0%
CAAGGAAACTTTCATTCTCT+TGG - chr3_3:67980853-67980872 Msa0466860:CDS 35.0%
! TTGGATAAAGACTTGCTTCT+TGG - chr3_3:67980872-67980891 Msa0466860:intron 35.0%
ACATTATTGACCTTCAACGT+GGG + chr3_3:67980918-67980937 None:intergenic 35.0%
AACATTATTGACCTTCAACG+TGG + chr3_3:67980919-67980938 None:intergenic 35.0%
TTATGATAGCTGAACAAACC+AGG - chr3_3:67981080-67981099 Msa0466860:intron 35.0%
CTAGTTTATAAGTAGGTACC+TGG + chr3_3:67981101-67981120 None:intergenic 35.0%
GCTCCAACATTTCTTATTGA+AGG - chr3_3:67981132-67981151 Msa0466860:intron 35.0%
ACGCCTTCAATAAGAAATGT+TGG + chr3_3:67981138-67981157 None:intergenic 35.0%
GTAAATGTAATCACATGCTC+CGG + chr3_3:67981163-67981182 None:intergenic 35.0%
TTATTGAGGATGCATGTGTT+GGG - chr3_3:67981284-67981303 Msa0466860:intron 35.0%
! CAACTTGCTTTACTTGGATA+TGG + chr3_3:67981330-67981349 None:intergenic 35.0%
CATATCCAAGTAAAGCAAGT+TGG - chr3_3:67981328-67981347 Msa0466860:intron 35.0%
TATGCAAGCATATGAGACTA+AGG + chr3_3:67981623-67981642 None:intergenic 35.0%
!! TTTTCCAGAACTTGTTCCAT+GGG + chr3_3:67981746-67981765 None:intergenic 35.0%
CTAGATATAAAAGCTTTCCC+CGG - chr3_3:67981837-67981856 Msa0466860:intron 35.0%
TAGATATAAAAGCTTTCCCC+GGG - chr3_3:67981838-67981857 Msa0466860:intron 35.0%
AGACTTCTAGATGAAAAACC+CGG + chr3_3:67981859-67981878 None:intergenic 35.0%
AGTGATCAAAAGTGAGATCA+AGG + chr3_3:67981927-67981946 None:intergenic 35.0%
!!! TTTTTTTGTTAGCATAGCCG+TGG - chr3_3:67981950-67981969 Msa0466860:intron 35.0%
!!! GGAGTCTGTTTTGATAATGA+TGG - chr3_3:67981988-67982007 Msa0466860:intron 35.0%
!!! GACAGACCAGTTTTTTAAAC+AGG + chr3_3:67982087-67982106 None:intergenic 35.0%
TAGAACATCACTTCTGATAC+TGG - chr3_3:67982199-67982218 Msa0466860:intron 35.0%
AAGACAATGGTACATTACAC+AGG - chr3_3:67982276-67982295 Msa0466860:intron 35.0%
AGACAATGGTACATTACACA+GGG - chr3_3:67982277-67982296 Msa0466860:intron 35.0%
ATACAGGGAAATGCATGAAT+TGG - chr3_3:67982456-67982475 Msa0466860:intron 35.0%
ACAAGGAAACATGTGCATAA+CGG + chr3_3:67982648-67982667 None:intergenic 35.0%
GGTAGCATACACATTAAACA+AGG + chr3_3:67982665-67982684 None:intergenic 35.0%
TTAATGTGTATGCTACCCTA+AGG - chr3_3:67982668-67982687 Msa0466860:intron 35.0%
! TAAGTGCAAGTTGAACCTTA+GGG + chr3_3:67982686-67982705 None:intergenic 35.0%
GGATAAAGTGAGAAATTGTC+AGG + chr3_3:67982709-67982728 None:intergenic 35.0%
!! GAACTTGGCTTTTACAACTA+AGG + chr3_3:67982730-67982749 None:intergenic 35.0%
ATGGTAGAATTAATCTCGAG+AGG - chr3_3:67982776-67982795 Msa0466860:intron 35.0%
GAAGTAATGCTCATTTACCA+TGG + chr3_3:67982803-67982822 None:intergenic 35.0%
! ACAGTGTTCCTTCATTTTGA+AGG - chr3_3:67982980-67982999 Msa0466860:intron 35.0%
!! TTCCTTCATTTTGAAGGATG+TGG - chr3_3:67982986-67983005 Msa0466860:intron 35.0%
TGTCTTTCTTTGTCCTTGTT+AGG - chr3_3:67983176-67983195 Msa0466860:intron 35.0%
TTCTGTTCCATTCCATTCTT+GGG + chr3_3:67983204-67983223 None:intergenic 35.0%
TTTCTGTTCCATTCCATTCT+TGG + chr3_3:67983205-67983224 None:intergenic 35.0%
GAGTATTCTCTATTGTGAAG+GGG + chr3_3:67983283-67983302 None:intergenic 35.0%
GAGTTGTTAATTACTTGACG+TGG + chr3_3:67983382-67983401 None:intergenic 35.0%
!!! TGTGTTTTAGCCGTTATATG+CGG - chr3_3:67984499-67984518 Msa0466860:intron 35.0%
AGTCACCTGTCAAACAAATT+AGG + chr3_3:67984570-67984589 None:intergenic 35.0%
ATTTGTTTGACAGGTGACTT+TGG - chr3_3:67984571-67984590 Msa0466860:CDS 35.0%
AATAACACAAGCCAAGAATG+AGG - chr3_3:67984601-67984620 Msa0466860:CDS 35.0%
AAATACGCACATCATGAACA+AGG + chr3_3:67984720-67984739 None:intergenic 35.0%
TTTCTGCAGCTTATGCGTTA+CGG + chr3_3:67980580-67980599 None:intergenic 40.0%
AAAATCAATTGGTCAGTCCG+TGG - chr3_3:67980602-67980621 Msa0466860:three_prime_UTR 40.0%
TCATGATATTTCGGAACGTG+AGG + chr3_3:67980734-67980753 None:intergenic 40.0%
TACTCTTGTTCGAGAGAAAG+GGG + chr3_3:67980800-67980819 None:intergenic 40.0%
GATACTCTTGTTCGAGAGAA+AGG + chr3_3:67980802-67980821 None:intergenic 40.0%
CATTATTGACCTTCAACGTG+GGG + chr3_3:67980917-67980936 None:intergenic 40.0%
ATTTCAACCTCTGCTATTCG+AGG + chr3_3:67981061-67981080 None:intergenic 40.0%
! TTTCTTATTGAAGGCGTGTC+CGG - chr3_3:67981141-67981160 Msa0466860:intron 40.0%
! TTGAAGCACTAACACAGAGA+CGG + chr3_3:67981244-67981263 None:intergenic 40.0%
CTTATTGAGGATGCATGTGT+TGG - chr3_3:67981283-67981302 Msa0466860:intron 40.0%
! CAACACCAACTTGCTTTACT+TGG + chr3_3:67981336-67981355 None:intergenic 40.0%
!! GTTTTCCAGAACTTGTTCCA+TGG + chr3_3:67981747-67981766 None:intergenic 40.0%
ACTTCTAGATGAAAAACCCG+GGG + chr3_3:67981857-67981876 None:intergenic 40.0%
GACTTCTAGATGAAAAACCC+GGG + chr3_3:67981858-67981877 None:intergenic 40.0%
!! GCCTTTGCTATTGAGAAGTT+GGG - chr3_3:67982010-67982029 Msa0466860:intron 40.0%
!! CCTTTGCTATTGAGAAGTTG+GGG - chr3_3:67982011-67982030 Msa0466860:intron 40.0%
! GTTGGGGATCAAAACTTTTC+AGG - chr3_3:67982027-67982046 Msa0466860:intron 40.0%
CACTTCTGATACTGGATAAC+TGG - chr3_3:67982207-67982226 Msa0466860:intron 40.0%
ACACAGGGAAATACATGAAC+TGG - chr3_3:67982292-67982311 Msa0466860:intron 40.0%
CACAGGGAAATACATGAACT+GGG - chr3_3:67982293-67982312 Msa0466860:intron 40.0%
CTGGGTCAAAATGTAACACT+CGG - chr3_3:67982311-67982330 Msa0466860:intron 40.0%
CACTTCTGATACTGGATAAC+TGG - chr3_3:67982207-67982226 Msa0466860:intron 40.0%
ACATGTGCATAACGGTTTAG+CGG + chr3_3:67982640-67982659 None:intergenic 40.0%
! GTAAGTGCAAGTTGAACCTT+AGG + chr3_3:67982687-67982706 None:intergenic 40.0%
AATTAATCTCGAGAGGACCA+TGG - chr3_3:67982783-67982802 Msa0466860:intron 40.0%
TCAGCATTGCATGTAGTTCA+AGG - chr3_3:67982914-67982933 Msa0466860:intron 40.0%
GGCACATATGCATTGTATCT+AGG - chr3_3:67982935-67982954 Msa0466860:intron 40.0%
GACCACATCCTTCAAAATGA+AGG + chr3_3:67982991-67983010 None:intergenic 40.0%
TTAGGAACCCAAGAATGGAA+TGG - chr3_3:67983194-67983213 Msa0466860:intron 40.0%
TATTGTGAAGGGGAAACTCT+TGG + chr3_3:67983273-67983292 None:intergenic 40.0%
CAAGAGACTGTTAGGAACTT+AGG + chr3_3:67983445-67983464 None:intergenic 40.0%
ACTTGAAGCAAGAGACTGTT+AGG + chr3_3:67983453-67983472 None:intergenic 40.0%
TAACAGTCTCTTGCTTCAAG+TGG - chr3_3:67983452-67983471 Msa0466860:CDS 40.0%
! TAGCTTGTCGATTGATGTAG+AGG - chr3_3:67983511-67983530 Msa0466860:intron 40.0%
AAACCACTTGAGAAGAGAGA+GGG + chr3_3:67983783-67983802 None:intergenic 40.0%
AATCCACAAGCTATGTCAAC+AGG + chr3_3:67983824-67983843 None:intergenic 40.0%
! ATAGCTTGTGGATTTGATGC+AGG - chr3_3:67983830-67983849 Msa0466860:intron 40.0%
! TAGCTTGTGGATTTGATGCA+GGG - chr3_3:67983831-67983850 Msa0466860:intron 40.0%
GGTAATACTGGTCCCTTACA+TGG - chr3_3:67980706-67980725 Msa0466860:three_prime_UTR 45.0%
GAACGTGAGGTTCCATGTAA+GGG + chr3_3:67980721-67980740 None:intergenic 45.0%
CAGTGGCTTGAAGAAGAGAA+GGG + chr3_3:67980769-67980788 None:intergenic 45.0%
TTGTTCGAGAGAAAGGGGTT+CGG + chr3_3:67980795-67980814 None:intergenic 45.0%
AGTATCAGATGCAACTCTCC+TGG - chr3_3:67980816-67980835 Msa0466860:CDS 45.0%
CTTCTTGGAACCTTCTCTCA+TGG - chr3_3:67980887-67980906 Msa0466860:intron 45.0%
CCAACCATAGCTGCAAATAG+CGG + chr3_3:67981004-67981023 None:intergenic 45.0%
CAATGATCCTCGAATAGCAG+AGG - chr3_3:67981051-67981070 Msa0466860:intron 45.0%
ACGGAAACACGCAAATACAC+CGG + chr3_3:67981660-67981679 None:intergenic 45.0%
TAAACCATTCCACAGACCCA+TGG - chr3_3:67981727-67981746 Msa0466860:intron 45.0%
!! GGCCTTTGCTATTGAGAAGT+TGG - chr3_3:67982009-67982028 Msa0466860:intron 45.0%
CCCCAACTTCTCAATAGCAA+AGG + chr3_3:67982014-67982033 None:intergenic 45.0%
CCATTCTTGGGTTCCTAACA+AGG + chr3_3:67983192-67983211 None:intergenic 45.0%
CCTTGTTAGGAACCCAAGAA+TGG - chr3_3:67983189-67983208 Msa0466860:intron 45.0%
GAAACCACTTGAGAAGAGAG+AGG + chr3_3:67983784-67983803 None:intergenic 45.0%
TCTCCTGTTGACATAGCTTG+TGG - chr3_3:67983818-67983837 Msa0466860:intron 45.0%
AGCTTGTGGATTTGATGCAG+GGG - chr3_3:67983832-67983851 Msa0466860:intron 45.0%
ACCAAGCATCTCCTCATTCT+TGG + chr3_3:67984615-67984634 None:intergenic 45.0%
! TGAGGAGATGCTTGGTTTGA+TGG - chr3_3:67984619-67984638 Msa0466860:CDS 45.0%
! GAGGAGATGCTTGGTTTGAT+GGG - chr3_3:67984620-67984639 Msa0466860:CDS 45.0%
CGTGTGAACCCATCTTAACA+AGG + chr3_3:67984868-67984887 None:intergenic 45.0%
GGAACGTGAGGTTCCATGTA+AGG + chr3_3:67980722-67980741 None:intergenic 50.0%
GCAGTGGCTTGAAGAAGAGA+AGG + chr3_3:67980770-67980789 None:intergenic 50.0%
CTTGTTGAGATCACGTGACC+AGG + chr3_3:67980837-67980856 None:intergenic 50.0%
CTGGTCACGTGATCTCAACA+AGG - chr3_3:67980835-67980854 Msa0466860:CDS 50.0%
! TGTGCCGCTATTTGCAGCTA+TGG - chr3_3:67980997-67981016 Msa0466860:intron 50.0%
CCGCTATTTGCAGCTATGGT+TGG - chr3_3:67981001-67981020 Msa0466860:intron 50.0%
TGTTCCATGGGTCTGTGGAA+TGG + chr3_3:67981734-67981753 None:intergenic 50.0%
! GAACTTGTTCCATGGGTCTG+TGG + chr3_3:67981739-67981758 None:intergenic 50.0%
CAGACCCATGGAACAAGTTC+TGG - chr3_3:67981739-67981758 Msa0466860:intron 50.0%
TGTTAGCATAGCCGTGGCAA+AGG - chr3_3:67981956-67981975 Msa0466860:intron 50.0%
CCTTTCCAAGTCCTTTGCCA+CGG + chr3_3:67981970-67981989 None:intergenic 50.0%
TGTGAAGGGGAAACTCTTGG+AGG + chr3_3:67983270-67983289 None:intergenic 50.0%
TCTCCCTCTCTCTTCTCAAG+TGG - chr3_3:67983777-67983796 Msa0466860:intron 50.0%
GCCAAGAATGAGGAGATGCT+TGG - chr3_3:67984611-67984630 Msa0466860:CDS 50.0%
AGAAAGGGGTTCGGAAGCAG+TGG + chr3_3:67980786-67980805 None:intergenic 55.0%
GTGGGGTTGACCATGAGAGA+AGG + chr3_3:67980900-67980919 None:intergenic 55.0%
CATGGTCAACCCCACGTTGA+AGG - chr3_3:67980905-67980924 Msa0466860:intron 55.0%
CATAGCCGTGGCAAAGGACT+TGG - chr3_3:67981962-67981981 Msa0466860:intron 55.0%
CCGTGGCAAAGGACTTGGAA+AGG - chr3_3:67981967-67981986 Msa0466860:intron 55.0%
GCTGCGCTTTGCTCTTGCCA+CGG + chr3_3:67980622-67980641 None:intergenic 60.0%
GTGGCAAGAGCAAAGCGCAG+CGG - chr3_3:67980621-67980640 Msa0466860:three_prime_UTR 60.0%
GCAAGAGCAAAGCGCAGCGG+CGG - chr3_3:67980624-67980643 Msa0466860:three_prime_UTR 65.0%
AGCGCAGCGGCGGAGTTCGA+TGG - chr3_3:67980634-67980653 Msa0466860:three_prime_UTR 70.0%
GCAGCGGCGGAGTTCGATGG+CGG - chr3_3:67980637-67980656 Msa0466860:three_prime_UTR 70.0%
! GCGGCGGAGTTCGATGGCGG+CGG - chr3_3:67980640-67980659 Msa0466860:three_prime_UTR 75.0%
! GCGGAGTTCGATGGCGGCGG+CGG - chr3_3:67980643-67980662 Msa0466860:three_prime_UTR 75.0%
! GAGTTCGATGGCGGCGGCGG+CGG - chr3_3:67980646-67980665 Msa0466860:three_prime_UTR 75.0%
GCGGCGGCGGCGAGGAAGAA+AGG - chr3_3:67980659-67980678 Msa0466860:three_prime_UTR 75.0%
!! CGATGGCGGCGGCGGCGGCG+AGG - chr3_3:67980651-67980670 Msa0466860:three_prime_UTR 90.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 67980563 67984894 67980563 ID=Msa0466860;Name=Msa0466860
chr3_3 mRNA 67980563 67984894 67980563 ID=Msa0466860-mRNA-1;Parent=Msa0466860;Name=Msa0466860-mRNA-1;_AED=0.10;_eAED=0.10;_QI=90|0.5|0.6|1|1|1|5|230|179
chr3_3 exon 67980563 67980873 67980563 ID=Msa0466860-mRNA-1:exon:15670;Parent=Msa0466860-mRNA-1
chr3_3 exon 67981567 67981605 67981567 ID=Msa0466860-mRNA-1:exon:15669;Parent=Msa0466860-mRNA-1
chr3_3 exon 67983409 67983495 67983409 ID=Msa0466860-mRNA-1:exon:15668;Parent=Msa0466860-mRNA-1
chr3_3 exon 67984385 67984443 67984385 ID=Msa0466860-mRNA-1:exon:15667;Parent=Msa0466860-mRNA-1
chr3_3 exon 67984531 67984894 67984531 ID=Msa0466860-mRNA-1:exon:15666;Parent=Msa0466860-mRNA-1
chr3_3 five_prime_UTR 67984805 67984894 67984805 ID=Msa0466860-mRNA-1:five_prime_utr;Parent=Msa0466860-mRNA-1
chr3_3 CDS 67984531 67984804 67984531 ID=Msa0466860-mRNA-1:cds;Parent=Msa0466860-mRNA-1
chr3_3 CDS 67984385 67984443 67984385 ID=Msa0466860-mRNA-1:cds;Parent=Msa0466860-mRNA-1
chr3_3 CDS 67983409 67983495 67983409 ID=Msa0466860-mRNA-1:cds;Parent=Msa0466860-mRNA-1
chr3_3 CDS 67981567 67981605 67981567 ID=Msa0466860-mRNA-1:cds;Parent=Msa0466860-mRNA-1
chr3_3 CDS 67980793 67980873 67980793 ID=Msa0466860-mRNA-1:cds;Parent=Msa0466860-mRNA-1
chr3_3 three_prime_UTR 67980563 67980792 67980563 ID=Msa0466860-mRNA-1:three_prime_utr;Parent=Msa0466860-mRNA-1
Gene Sequence

>Msa0466860

ATGGCGGCGGCGGCGGCGAGGAAGAAAGGAAATGTGAGTTTAGAAGGAAAGAAGGTAATACTGGTCCCTTACATGGAACCTCACGTTCCGAAATATCATGAATGGATGCAAGACCCTTCTCTTCTTCAAGCCACTGCTTCCGAACCCCTTTCTCTCGAACAAGAGTATCAGATGCAACTCTCCTGGTCACGTGATCTCAACAAGGAAACTTTCATTCTCTTGGATAAAGACTTGCTTCTTGGAACCTTCTCTCATGGTCAACCCCACGTTGAAGCTATGGTTGGTGATGTAAATATTTTCATGAATGATATCAATGATCCTCGAATAGCAGAGCATAGCCGTGGCAAAGGACTTGGAAAGGAGTCTGTTTTGATAATGATGGCCTTTGCTATTGAGAAGTTGGGGATCAAAACTTTTCAGGGGTTTGTGCAAACTTCACATAGCAACATTTTCAAAGAGGTGACTTTGGAGTTGCAAATAACACAAGCCAAGAATGAGGAGATGCTTGGTTTGATGGGTACTGTGATTAAACACACATAA
Protein sequence

>Msa0466860

MAAAAARKKGNVSLEGKKVILVPYMEPHVPKYHEWMQDPSLLQATASEPLSLEQEYQMQLSWSRDLNKETFILLDKDLLLGTFSHGQPHVEAMVGDVNIFMNDINDPRIAEHSRGKGLGKESVLIMMAFAIEKLGIKTFQGFVQTSHSNIFKEVTLELQITQAKNEEMLGLMGTVIKHT*