AlfalfaGEDB | Msa0467140

M. sativa cultivar zhongmu-4 / Msa0467140

KEGG: K09422
GO: GO:0000003 GO:0000902 GO:0000904 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010026 GO:0010090 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0048316 GO:0048354 GO:0048359 GO:0048468 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090626 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0467140	AES71860.2	90.588	85	5	2	1	82	219	303	3.73e-43	151

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0467140	G7J9M1	90.588	85	5	2	1	82	219	303	1.78e-43	151

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0207320	Msa0467140	0.818988	1.470203e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0467140	MtrunA17_Chr3g0121241	90.588	85	5	2	1	82	220	304	3.59e-47	151

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATGTGTTTACTTCATTTC+TGG	0.117632	3_3:+68373125	Msa0467140:CDS
AAGCTTTCCCGTCTTGGTTT+TGG	0.183376	3_3:-68373269	None:intergenic
AACAACAACAGAGGGGATTA+TGG	0.374480	3_3:+68373072	Msa0467140:CDS
TGCTGCACCACCAGCTTTGT+GGG	0.386603	3_3:+68373212	Msa0467140:CDS
CTGCTGCACCACCAGCTTTG+TGG	0.390612	3_3:+68373211	Msa0467140:CDS
ATCATGAAGCTTTCCCGTCT+TGG	0.395480	3_3:-68373275	None:intergenic
ATAATCCCCTCTGTTGTTGT+TGG	0.419997	3_3:-68373070	None:intergenic
GACAGTAGTAATAACAATTA+CGG	0.457012	3_3:+68373096	Msa0467140:CDS
ATTTCACCCAAAACCAAGAC+GGG	0.547074	3_3:+68373262	Msa0467140:CDS
AGCGGCGATTCCCACAAAGC+TGG	0.550946	3_3:-68373222	None:intergenic
TGCTTACCAACAACAACAGA+GGG	0.561719	3_3:+68373064	Msa0467140:CDS
GGCGATTCCCACAAAGCTGG+TGG	0.607315	3_3:-68373219	None:intergenic
AATTTCACCCAAAACCAAGA+CGG	0.622349	3_3:+68373261	Msa0467140:CDS
GGTGCAGCAGATGAGATCAA+AGG	0.650426	3_3:-68373198	None:intergenic
GTGCTTACCAACAACAACAG+AGG	0.695079	3_3:+68373063	Msa0467140:CDS
TTATGAGTAGACATGATGAG+CGG	0.724968	3_3:-68373240	None:intergenic
AGTAGTAATAACAATTACGG+CGG	0.747388	3_3:+68373099	Msa0467140:CDS
GCTTACCAACAACAACAGAG+GGG	0.820454	3_3:+68373065	Msa0467140:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GACAGTAGTAATAACAATTA+CGG	+	chr3_3:68373096-68373115	Msa0467140:CDS	25.0%
	AGTAGTAATAACAATTACGG+CGG	+	chr3_3:68373099-68373118	Msa0467140:CDS	30.0%
	CGATGTGTTTACTTCATTTC+TGG	+	chr3_3:68373125-68373144	Msa0467140:CDS	35.0%
	TTATGAGTAGACATGATGAG+CGG	-	chr3_3:68373243-68373262	None:intergenic	35.0%
	AATTTCACCCAAAACCAAGA+CGG	+	chr3_3:68373261-68373280	Msa0467140:CDS	35.0%
	TGCTTACCAACAACAACAGA+GGG	+	chr3_3:68373064-68373083	Msa0467140:CDS	40.0%
	ATAATCCCCTCTGTTGTTGT+TGG	-	chr3_3:68373073-68373092	None:intergenic	40.0%
	AACAACAACAGAGGGGATTA+TGG	+	chr3_3:68373072-68373091	Msa0467140:CDS	40.0%
	ATTTCACCCAAAACCAAGAC+GGG	+	chr3_3:68373262-68373281	Msa0467140:CDS	40.0%
	GTGCTTACCAACAACAACAG+AGG	+	chr3_3:68373063-68373082	Msa0467140:CDS	45.0%
	GCTTACCAACAACAACAGAG+GGG	+	chr3_3:68373065-68373084	Msa0467140:CDS	45.0%
!!	AGCTTTCCCGTCTTGGTTTT+GGG	-	chr3_3:68373271-68373290	None:intergenic	45.0%
!	AAGCTTTCCCGTCTTGGTTT+TGG	-	chr3_3:68373272-68373291	None:intergenic	45.0%
	GGTGCAGCAGATGAGATCAA+AGG	-	chr3_3:68373201-68373220	None:intergenic	50.0%
!	TGCTGCACCACCAGCTTTGT+GGG	+	chr3_3:68373212-68373231	Msa0467140:CDS	55.0%
	CTGCTGCACCACCAGCTTTG+TGG	+	chr3_3:68373211-68373230	Msa0467140:CDS	60.0%
	GGCGATTCCCACAAAGCTGG+TGG	-	chr3_3:68373222-68373241	None:intergenic	60.0%
	AGCGGCGATTCCCACAAAGC+TGG	-	chr3_3:68373225-68373244	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	68373048	68373296	68373048	ID=Msa0467140;Name=Msa0467140
chr3_3	mRNA	68373048	68373296	68373048	ID=Msa0467140-mRNA-1;Parent=Msa0467140;Name=Msa0467140-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|82
chr3_3	exon	68373048	68373296	68373048	ID=Msa0467140-mRNA-1:exon:15797;Parent=Msa0467140-mRNA-1
chr3_3	CDS	68373048	68373296	68373048	ID=Msa0467140-mRNA-1:cds;Parent=Msa0467140-mRNA-1

Gene Sequence	>Msa0467140 ATGGATAATCATCATGTGCTTACCAACAACAACAGAGGGGATTATGGTGACAGTAGTAATAACAATTACGGCGGTGACGATGTGTTTACTTCATTTCTGGATTCATTGATCAACGACGATGCATTTGCTGCACACAGATCAGAGAATGATCCTTTGATCTCATCTGCTGCACCACCAGCTTTGTGGGAATCGCCGCTCATCATGTCTACTCATAATTTCACCCAAAACCAAGACGGGAAAGCTTCATGA
Protein sequence	>Msa0467140 MDNHHVLTNNNRGDYGDSSNNNYGGDDVFTSFLDSLINDDAFAAHRSENDPLISSAAPPALWESPLIMSTHNFTQNQDGKAS*